Algal Research-Biomass Biofuels and Bioproducts
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2025
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IF 4.5
Juan Liu,Shiqing Sun,Kangyu Chen,Shumei Gao,Dixing Chen,Yuejin Zhang,Changwei Hu
Fungi-microalgae consortia have great potential in microalgae harvesting and wastewater treatment. However, the symbiotic mechanisms underlying diffusible signaling metabolite production by fungi and microalgae remain unclear. Here, the positive impacts of Clonostachys rosea on Chlorella sp. were investigated using metabolomics and transcriptomics during a 5-day non-contact co-culture experiment. Co-cultured Chlorella sp. had a significantly higher growth rate and better photosynthetic performance than its mono-culture equivalent. The metabolites played important roles in the up-regulation of energy and amino acid metabolism processes in algal cells, whilst transcriptomics showed the up-regulation of genes involved in photosynthesis and signaling molecules. The biosynthesis of tryptophan and indole-3-acetic acid (IAA) was up-regulated in both algal cells and fungi, indicating that IAA is an important diffusible signaling molecule for algal growth regulation. This work provides novel insights into the signaling molecule interactions between fungi and microalgae in fungi-microalgae consortia.
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高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2021
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IF 3.493
Yingying Zhou,Yu Wang,Xi Chen,Jinhui Feng,Min Wang,Qiaqing Wu,Dunming Zhu
Enzymatic stereospecific reduction of 17-oxosteroids offers an attractive approach to access 17β-hydroxysteroids of pharmaceutical importance. In this study, by adjusting the flexibility of α6-helix at the substrate entrance of the alcohol dehydrogenase from Ralstonia sp. (RasADH), the catalytic activity toward the stereospecific 17β-reduction of androstenedione was improved without sacrifice of the enantioselectivity. Among the mutants, F205I and F205A exhibited up to 623- and 523-fold improvement in catalytic efficiency , respectively, towards a range of different 17-oxosteroids compared to the wild-type enzyme. The corresponding 17β-hydroxysteroids were prepared in optically pure form with high space-time productivity and isolated yields using F205I as the biocatalyst, indicating that these mutants are promising biocatalysts for this useful transformation. These results suggest that modulating the flexibility of the active site lid offers an effective approach to engineer alcohol dehydrogenase for accommodating bulky steroidal substrates.
研究领域:
生物化学
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药物合成
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BIORESOURCE TECHNOLOGY
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2021
·
IF 9.642
Xinyue Zhao,Yuting Zhang,Hanyi Jiang,Hailian Zang,Yue Wang,Shanshan Sun,Chunyan Li
Vanillin is a natural flavoring agent that is widely used in the bioengineering industry. To enable sustainable development, joint consideration of bacterial performance and negative environmental impacts are critical to vanillin biosynthesis. In this study, a cold shock protein (csp) gene was upregulated for maintaining stable growth in Arthrobacter sp. C2 responding to vanillin and cold stress. Furthermore, the recombinant strain C2 was constructed by simultaneously deleting the xylC gene encoding benzaldehyde dehydrase and overexpressing the pchF gene encoding vanillyl alcohol oxidase and achieved a maximum vanillin productivity of 0.85 mg/g DCW/h with alkaline lignin as the substrate. Finally, this process generated an environmental impact value of 25.05, which was the lowest environmental impact achieved according to life cycle assessment (LCA). Improvement strategies included reducing electricity consumption and replacing chemicals. This study achieved the development of an effective strategy, and future studies should focus on precise vanillin biosynthesis methods for large-scale application.
研究领域:
生物技术
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环境科学
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化学工程
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高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2016
·Vol. 97
·期 4
·1342-1348
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IF 2.076
Cuiqiong Wang,Zhongyuan Li,Hui Wang,Haiyan Qiu,Minghui Zhang,Shuang Li,Xuegang Luo,Yajian Song,Hao Zhou,Wenjian Ma,Tongcun Zhang
BACKGROUND Contamination of food and feed by aflatoxin B1 (AFB1) poses serious economic and health problems worldwide, so the development of biological methods for effective AFB1 degradation is strongly required. RESULTS Among three AFB1-degrading microorganisms isolated from moldy peanut, Fusarium sp. WCQ3361 could remove AFB1 extremely effectively, with a degradation ratio of 70.20% after 1 min and 95.38% after 24 h. Its degradation ratio was not much affected by temperature change (0–90 °C) and it also displayed excellent thermostability, maintaining 99.40% residual activity after boiling for 10 min. Since protease K could reduce the AFB1 degradation ratio by 55.15%, it is proposed that the effective component for AFB1 degradation is a protein. The AFB1 degradation ability of Fusarium sp. WCQ3361 was further verified by feed stock detoxification and the MTT test with HepG2 cells. In addition, no degradation products were detected by preliminary liquid chromatography/mass spectrometry, suggesting that AFB1 might be metabolized to products with different chemical characteristics from AFB1. CONCLUSION Fusarium sp. WCQ3361 is the first reported AFB1 degradation fungus belonging to the genus Fusarium with broad working temperature range, excellent thermostability and high activity, which provides a potential highly useful solution for dealing with AFB1 contamination in the human diet and animal feed. © 2016 Society of Chemical Industry
研究领域:
食品科学
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微生物学
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毒理学
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高影响因子
BIORESOURCE TECHNOLOGY
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2020
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IF 7.539
Zhe Dou,Guochao Xu,Ye Ni
A novel carboxylesterase Ac Est1 was identified from Acinetobacter sp. JNU9335 with high efficiency in the biosynthesis of chiral precursor of Edoxaban through kinetic resolution of methyl 3-cyclohexene-1-carboxylate (CHCM). Sequence analysis revealed Ac Est1 belongs to family IV of esterolytic enzymes and exhibits <40% identities with known carboxylesterases. The optimum pH and temperature of recombinant Ac Est1 are 8.0 and 40 °C. Substrate spectrum analysis indicated that Ac Est1 prefers substrates with short acyl and alcohol groups. Ac Est1 was highly active in the hydrolysis of CHCM with k cat of 1153 s −1 and displayed high substrate tolerance. As much as 2.0 M (280 g·L −1 ) CHCM could be enantioselectively hydrolyzed into ( S )-CHCM by merely 0.08 g·L −1 Ac Est1 with ee s of >99% ( S ) and substrate to catalyst ratio (S/C) of 3500 g·g −1 . These results indicate that the novel Ac Est1 is a promising biocatalyst in the synthesis of chiral carboxylic acids.
研究领域:
生物催化
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酶学
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药物化学
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SCIENCE OF THE TOTAL ENVIRONMENT
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2018
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IF 4.61
Hai-Ming Zhao,Rui-Wen Hu,Huan Du,Xiao-Ping Xin,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Jie-Sheng Liu,Dong-Mei Zhou,Ming-Hung Wong,Zhen-Li He
Microbial degradation is considered the most promising method for removing phthalate acid esters (PAEs) from polluted environments; however, a comprehensive genomic understanding of the entire PAE catabolic process is still lacking. In this study, the repertoire of PAE catabolism genes in the metabolically versatile bacterium Rhodococcus sp. 2G was examined using genomic, metabolic, and bioinformatic analyses. A total of 4930 coding genes were identified from the 5.6 Mb genome of the 2G strain, including 337 esterase/hydrolase genes and 48 transferase and decarboxylase genes that were involved in hydrolysing PAEs into phthalate acid (PA) and decarboxylating PA into benzoic acid (BA). One gene cluster ( xyl ) responsible for transforming BA into catechol and two catechol-catabolism gene clusters controlling the ortho ( cat ) and meta ( xyl & mhp ) cleavage pathways were also identified. The proposed PAE catabolism pathway and some key degradation genes were validated by intermediate-utilising tests and real-time quantitative polymerase chain reaction. Our results provide novel insight into the mechanisms of PAE biodegradation at the molecular level and useful information on gene resources for future studies.
研究领域:
微生物学
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环境科学
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基因组学
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Journal of Environmental Chemical Engineering
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2026
·Vol. 14
·期 6
·125520
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IF 7.5
Liping Yang,Nuerbiye Yisimayi,Wanyi Zhang,Mingyang Sun,Xinyue Tang,Lingna Chen,Yongkun Chen,Ai Shanjiang
A highly tolerant Chlorella sp. achieved efficient lambda-cyhalothrin transformation. • Multi-omics revealed coordinated detoxification and metabolic remodeling. • Intracellular–extracellular remodeling drove adaptive LC responses. • XDA-R55 treatment reduced lambda-cyhalothrin-induced oxidative stress and toxicity. • Mechanisms underlying microalgal pesticide adaptation were elucidated. Lambda-cyhalothrin (LC), a widely used pyrethroid insecticide, poses persistent ecological risks due to its toxicity and environmental persistence. In this study, a microalga strain, Chlorella sp. XDA-R55, was investigated as a potential biological platform for LC-contaminated water remediation. XDA-R55 maintained active growth under 20 mg/L LC exposure and promoted 96.6% LC removal within 6 days without external carbon supplementation. Physiological analyses revealed that XDA-R55 alleviated LC-induced oxidative stress by enhancing antioxidant defense systems and maintaining cellular redox homeostasis. Integrated transcriptomic and metabolomic analyses indicated that XDA-R55 facilitates the detoxification and transformation of LC by upregulating genes associated with glutathione metabolism, biosynthesis of secondary metabolites, and ABC transporters. Several putative transformation products, such as 4-hydroxybenzaldehyde, were identified and exhibited transient accumulation followed by decline. Ecological safety assessments demonstrated that water treated with XDA-R55 significantly alleviated oxidative stress, metabolic disturbances, and immune suppression in zebrafish, with most biomarkers recovering to levels comparable to those in the control group. Moreover, as a photoautotrophic organism, XDA-R55 facilitated LC removal and transformation without external carbon supplementation, providing a sustainable and environmentally compatible strategy for pesticide-contaminated water remediation. These findings provide mechanistic insights into microalgal adaptation and LC transformation under pesticide stress, and highlight the potential of Chlorella sp. XDA-R55 for mitigating the ecological risks associated with LC-contaminated water. Download: Download high-res image (189KB) Download: Download full-size image
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ACS Applied Materials & Interfaces
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2025
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IF 8.2
Shuai Wang,Yuqi Chen,Shuyan Zheng,Yanan Yuan,Kexin Du,Min Cui,Yue Wu,Ping Li,Wei Hu,Jingui Wang,Yingshu Guo,Zexing Wu,Yanli Zhao
The incorporation of sp-hybridized nitrogen arisen from graphdiyne (GDY) into the catalyst structure significantly improves the electrocatalytic efficiency of zinc–air batteries by modulating the electronic properties of the active sites, thereby accelerating reaction kinetics and enhancing conductivity. This work highlights the efficacy of the Co–N-GDY catalyst, where sp-N moieties in GDY serving as anchoring centers for cobalt species, which demonstrates exceptional performance in the oxygen reduction reaction, achieving a half-wave potential of 0.85 V, along with high oxygen evolution reaction performance that surpasses that of commercial Pt/C + IrO2-based batteries. The Co–N-GDY catalyst also exhibits commendable cycling stability with minimal fluctuation in voltage difference, indicating its suitability for practical applications in advanced energy storage systems. These findings underscore the importance of developing cost-effective and durable M–N–C catalysts to enhance the commercial viability and sustainability of zinc–air battery technology.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2025
·
IF 3.7
Jing Wu,Zhenggang Han,Pengrong Li,Jing Li,Yuanyuan Chen,Shangbo Ning,Hong-jun Chao,Xue-wang Gao,Dazhong Yan
Cyclohexylamine oxidase is a member of amine oxidases that catalyzes the conversion of cyclohexylamine to cyclohexanone. In our previous work, the enzymatic activity assay of cyclohexylamine oxidase CHAO YT-02 indicated that its specific activity towards cyclohexylamine of CHAO YT-02 was ten times higher than that of its homolog CHAO IH-35A . In this study, the crystal structure of CHAO YT-02 was determined by the molecular replacement method at a resolution of 1.49 Å. The atomic structure revealed that the amino acid residues Leu302, Trp70, Phe197, Phe349, and Tyr440 constitute the active center pocket of the enzyme. Amino acid residues Ile180, Leu181, and Trp332 separate the active center pocket and an intermediate pocket. Moreover, a molecular dynamics (MD) simulation and the calculation of the binding free energy were performed to predict substrate entry and product release from cyclohexylamine oxidases. Single-amino acid substitution mutants (W70A, I180A, L181A, I208A, F197A, L302A, W332A, F349A, and Y440A) of CHAO YT-02 were constructed to investigate the role of these amino acid residues in enzymatic properties and substrate specificity. The results indicated that both the amino acid residues in the active center pocket and gating the two pockets affected the activity or substrate specificity of CHAO YT-02 . This study on the structure and catalytic mechanism of cyclohexylamine oxidase is beneficial to eliminating toxic amine compounds in the environment.
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MARINE BIOTECHNOLOGY
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2025
·Vol. 27
·期 1
·1-16
·
IF 2.8
Bai Ying,Yang Gan,Liu Tongde,Chen Fuyan,Xia Junhong
During wintering, red tilapia may develop variable black spots on their bodies, significantly reducing their market value. Understanding the mechanisms driving this phenomenon is essential for molecular improvements in body color. In this study, we investigated chromatin accessibility landscapes in the eyes of red tilapia with two distinct phenotypes (normal pure red and black spot) under wintering stress using ATAC-seq and RNA-seq analyses. We observed that approximately 32.7% of chromatin accessibility peaks were located in promoter regions, followed by intergenic regions (32.4%) and intronic regions (26.7%). One thousand two hundred twenty-nine differentially accessible regions (DARs) and 1448 differentially expressed genes (DEGs) were identified between the RS and DS groups. Notably, DEGs associated with melanin synthesis, including tyrp1 , tyr , tyrp1b , pmela , slc24a5 , and mlph , were significantly upregulated in the DS group, which aligns with the observed 1.85-fold increase in melanin content, compared to the RS group. 92 DEGs were associated with significant changes in chromatin accessibility between groups ( R 2 = 0.8059; p < 0.0001), indicating potential regulatory relationships. Interestingly, 23.92% of the DARs were located on the chromosome 3. Specifically, a 2.5-fold difference in average peak height on LG3: 11,215,273–11,217,225 were observed between DS and RS tilapia. In the region, transcription factors including HSF1 and HSF2 were identified as key regulators of chromatin structure and gene expression under wintering stress. Our findings reveal that dynamic chromatin accessibility in the eyes of red tilapia facilitates adaptation to wintering stress by regulating visual signaling, melanin production, and downstream pigmentation.
研究领域:
水产养殖学
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分子生物学
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遗传学
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高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
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2025
·Vol. 136
·期 9
·
IF 3.2
Song Kangjia,Liu Jie,Zhou Wenqi,Zhang Feifei,Fan Lei,Zhu Deyi,Zhang Xuanhui,Wang Zhi,Sun Jiazhen
AimsThis research aims to screen and identify a microbial strain capable of efficiently degrading waterborne polyurethane used in the biomedical field. Furthermore, the research seeks to enhance the strain’s degradation capability through ultraviolet mutagenesis and elucidate its enzymatic degradation mechanism.Methods and resultsWe successfully isolated the target strain from the soil sample and identified it as Bacillus sp. J-11. Under optimized culture conditions (substrate concentration of 0.4%, temperature of 30°C, and pH of 6.0), strain J-11 exhibited remarkable degradation capability towards biomedical-grade polyurethane emulsion (Baymedix® CD104), achieving a degradation efficiency of 78.2% within 4 days. Gel permeation chromatography (GPC) analysis demonstrated a progressive decrease in the molecular weight of the polyurethane film over time. Thermogravimetric analysis (TGA) further revealed a reduction in thermal stability, while extended depth field microscopy (EDFM) uncovered significant microscopic structural changes, including holes and collapse on the film surface. Additional degradation experiments conducted on commercial PU foam under co-carbon conditions showed that strain J-11 achieved a degradation rate of 37.6% within 30 days. Enzyme activity assays indicated that the hydrolytic and oxidative enzymes secreted by strain J-11 played a critical role in the degradation process.ConclusionsStrain J-11 exhibits highly efficient biodegradation potential through the secretion of hydrolases and oxidases, which break down the molecular chains of polyurethane. This study has elucidated its degradation pathway and provided a theoretical foundation for future mechanistic investigations and the management of biomedical material waste.
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BIORESOURCE TECHNOLOGY
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2025
·
IF 9
Shiye Zhu,Jiale Zhang,Anwei Chen,Youzheng Chai,Jianhua Zeng,Gen Lu,Ma Bai,Jihai Shao,Liang Peng,Si Luo
Agricultural expansion has led to the accumulation of neonicotinoid pesticides in water and soil, which are habitats for widespread cyanobacteria. However, the cyanobacteria-neonicotinoids interaction mechanisms remain unclear. This study first explores Thiamethoxam (THX) biodegradation by Nostoc sp. PCC7120, elucidating cytochrome P450 (CYP450)-mediated THX metabolism. Nostoc sp. PCC7120 could remove THX completely within 6 days mainly via biodegradation (75 %). While THX stimulates cell growth, evidence (damaged thylakoid membranes, lowered electron transfer efficiency, reduced photosynthetic pigments, and altered key gene expression) shows it disrupts photosynthesis. Furthermore, THX degradation involved ring opening, nitrate reduction, de-chlorination, and N -dealkylation; molecular dynamics simulations revealed THX stably binding (32.38 kcal/mol) to CYP450′s active site and primary degradation via hydroxylation and de-chlorination. Ecotoxicity assessments show desmethyl and urea metabolites are more toxic to non-target organisms and humans than the parent compound. This study elucidates THX removal mechanisms and environmental fate, highlighting cyanobacteria’s potential in bioremediation for agricultural wastewater.
研究领域:
环境科学
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微生物学
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生态毒理学
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生物技术
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农业化学
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计算生物学
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高影响因子
危险品化学品经营许可证（带存储）