NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 17
·8083-8089
·
IF 3.3
Zhaofei Liu,Lili Zhang,Yuhang Wei,Zhiming Chen
Rapid and sensitive detection of hazardous compounds is of great importance for sensors. This work presents the immobilization of laccase on Fe3O4@MF-CNTs nanocomposites (Fe3O4@MF-CNTs-lac) for the fast and sensitive detection of catechol. Electrochemical impedance spectroscopy (EIS) results revealed that the combined effect of Fe3O4@MF and carbon nanotubes (CNTs) facilitates electron transfer between laccase and the electrode. Amperometric curves indicated that the Fe3O4@MF-CNTs-Lac biosensor can respond rapidly to a substrate and detect catechol within 0.5 s. The Fe3O4@MF-CNTs-Lac biosensor possesses a low detection limit of 0.84 μM (S/N = 3) with a linear range of 1–3000 μM, as well as high sensitivity, remarkable organic compound tolerance, and good selectivity, reproducibility and stability. The catechol biosensing assays revealed that Fe3O4@MF-CNTs-Lac can be operated in acidic water environments. Most importantly, the Fe3O4@MF-CNTs-Lac biosensor exhibited reliable results in real water sample analysis. The outstanding biosensing performances reveal that the Fe3O4@MF-CNTs-Lac biosensor has a technical application in the treatment of catechol to avoid catechol pollution.
研究领域:
环境科学
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生物技术
·
电化学
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DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Wang Jianle,Zeng Gongchang,Deng Hong,Liu Xueming,Zhao Dongye
Remediation of soil contaminated by mixed heavy metals and metalloids has been a major challenge in the global environmental field. To address this critical issue, we tested a new technology for simultaneous immobilization of lead (Pb) and arsenic (As) in a field contaminated soil using a microwave-assisted FeSO 4 ·7H 2 O treatment process. The process was able to rapidly reduce the TCLP-based leachability of Pb from 12.74 to 0.1 mg L −1 and As from 2.704 to 0.002 mg L −1 (MW power = 800 W, Irradiation time = 20 min, and FeSO 4 ·7H 2 O = 4 wt%). The effects of FeSO 4 ·7H 2 O dosage, microwave power, and irradiation time were determined and optimized. After 365 days of curing under atmospheric conditions, the TCLP-leached concentration of Pb and As in the treated soil remained below the regulatory limits of 0.1 and 0.002 mg L −1 , respectively. The microwave irradiation promoted the formation of insoluble PbSO 4 (s) and Fe 3 (AsO 4 ) 2 ·8H 2 O(s), resulting in the long-term stability of Pb and As in the soil. The technology offers an effective alternative for remediation of Pb- and/or As-contaminated soil and groundwater.
研究领域:
环境科学
·
污染控制技术
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2024
·Vol. 108
·期 1
·1-12
·
IF 3.9
Chen Qianlin,Wang Bin,Pan Li
Abstract In this study, we successfully applied the strategy of combining tandem promoters and tandem signal peptides with overexpressing signal peptidase to efficiently express and produce γ-glutamyl peptidase (GGT) enzymes (BsGGT, BaGGT, and BlGGT) from Bacillus subtilis , Bacillus amyloliquefaciens , and Bacillus licheniformis in Bacillus subtilis ATCC6051Δ5. In order to avoid the problem of instability caused by duplicated strong promoters, we assembled tandem promoters of different homologous genes from different species. To achieve resistance marker-free enzyme in the food industry, we first removed the replication origin and corresponding resistance marker of Escherichia coli from the expression vector. The plasmid was then transformed into the B. subtilis host, and the Kan resistance gene in the expression plasmid was directly edited and silenced using the CRISPR/Cas9n-AID base editing system. As a result, a recombinant protein expression carrier without resistance markers was constructed, and the enzyme activity of the BlGGT strain during shake flask fermentation can reach 53.65 U/mL. The recombinant BlGGT was immobilized with epoxy resin and maintained 82.8% enzyme activity after repeated use for 10 times and 87.36% enzyme activity after storage at 4 °C for 2 months. The immobilized BlGGT enzyme was used for the continuous synthesis of theanine with a conversion rate of 65.38%. These results indicated that our approach was a promising solution for improving enzyme production efficiency and achieving safe production of enzyme preparations in the food industry. Key points
• Efficient expression of recombinant proteins by a combination of dual promoter and dual signal peptide.
• Construction of small vectors without resistance markers in B. subtilis using CRISPR/Cas9n-AID editing system.
• The process of immobilizing BlGGT with epoxy resin was optimized.
研究领域:
微生物学
·
食品科学
·
分子生物学
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DOI
PubMed
高影响因子
Pigment & Resin Technology
·
2021
·Vol. 51
·期 5
·499-507
·
IF 1.263
Hongyi Tu,Donglei Liu,Zhenbin Chen,Chunli Liu
PurposeUsing a reversible addition fragmentation chain transfer reaction, a series of resins were prepared by using N, N-diethyl acrylamide (DEA), poly (ß-hydroxyethyl methacrylate) (PHEMA) as hydrophilic blocks and poly (glycidyl methacrylate) (PGMA) as hydrophobic blocks (and as a target for immobilizing penicillin G acylase [PGA]) and the low critical solution temperature (LCST) of which could be adjusted by changing the segment length of blocks.Design/methodology/approachTo make the catalytic conversion temperature of immobilized PGA fallen into the temperature range of the sol state of thermosensitive block resin, a type of thermosensitive block resin, i.e. PDEA-b-PHEMA-b-PGMA (DHGs) was synthesized to immobilize PGA, and the effect of segment order of block resin was investigated on the performance of PGA.FindingsCarrier prepared with monomers molar ratio of n(DEA) : n(HEMA): n(GMA) = 100: 49: 36 presented loading capacity (L) and enzyme activity recovery ratio (Ar) of 110 mg/g and 90%, respectively, and a block resin with LCST value of 33 °C was essential for keeping higher Ar of PGA.Originality/valuePGA has become an important biocatalyst in modern chemistry industry. However, disadvantages include difficulty in separation, poor repeatability and high cost, which limits the scope of PGA applications. The effective method is to immobilize the enzyme to the carrier, which could overcome the disadvantage of free enzyme.
研究领域:
生物化学
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材料科学
·
酶工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Jianxing Liu,Xiaotong Shen,Ziyan Zheng,Mingjie Li,Xinshu Zhu,Hui Cao,Caixia Cui
Due to the increasing quantities of phenolic compounds present in wastewater, the use of enzymatic degradation with the laccase has attracted much attention as a green option for their removal. In this work, we developed a novel immobilization technology using 3D bioprinting for laccase immobilization. The hydrogel mechanism properties were optimized by experimenting with different component ratios of sodium alginate (SA), acrylamide (AM), and hydroxyapatite (HA). The improved mechanism properties were validated by morphology pictures and rheology characteristics. The optimal AM:HA:SA ratio was determined to be 4:1.2:1. We then employed an extrusion-based bioprinting technique to prepare the immobilized laccase. The substrate conversion was increased with the addition of HA, which improved the permeability of the matrix, and proved to be suitable for immobilization. The resulting immobilized laccase was used for the biodegradation of p-chlorophenol. The effects of the initial substrate concentration, pH, and temperature were evaluated. The immobilized laccase exhibited good storage stability and reusability, retaining over 80% of its initial activity after 72 h of storage, and was able to be reused for seven batches. These results highlight that the immobilized laccase prepared by 3D bioprinting has great potential for use in the biodegradation of phenolic compounds.
研究领域:
环境科学
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生物技术
·
化学工程
·
材料科学
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DOI
PubMed
高影响因子
Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 6
·784-794
·
IF 4.5
Xu Junyang,Liu Guanhua,He Ying,Zhou Liya,Ma Li,Liu Yunting,Zheng Xiaobing,Gao Jing,Jiang Yanjun
In situ encapsulation is an effective way to synthesize enzyme@metal-organic framework biocatalysts; however, it is limited by the conditions of metal-organic framework synthesis and its acid-base stability. Herein, a biocatalytic platform with improved acid-base stability was constructed via a one-pot method using bismuth-ellagic acid as the carrier. Bismuth-ellagic acid is a green phenol-based metal-organic framework whose organic precursor is extracted from natural plants. After encapsulation, the stability, especially the acid-base stability, of amyloglucosidases@bismuth-ellagic acid was enhanced, which remained stable over a wide pH range (2–12) and achieved multiple recycling. By selecting a suitable buffer, bismuth-ellagic acid can encapsulate different types of enzymes and enable interactions between the encapsulated enzymes and cofactors, as well as between multiple enzymes. The green precursor, simple and convenient preparation process provided a versatile strategy for enzymes encapsulation.
研究领域:
生物技术
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催化
·
材料科学
·
酶学
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DOI
高影响因子
LANGMUIR
·
2026
·
IF 4.4
Yuebin Ye,Yundong Lai,Shibei Liu,Lei Ping,Yufei Zhang,Mingming Zheng
This study constructs an immobilized phospholipase system based on mesoporous silicon-based Janus microspheres and validates its exceptional performance in efficient interfacial biocatalysis. This system employs a Janus nanocarrier (HMSS-NH2-C8-Janus). The carrier was functionalized via a Pickering emulsion strategy, which grafted hydrophilic amino groups and hydrophobic octyl groups onto opposite hemispheres. This well-designed amphiphilicity not only facilitates effective immobilization of phospholipase B (PLB) but, more importantly, enables the resulting PLB@HMSS-NH2-C8-Janus to act as an excellent stabilizer for Pickering emulsions. Within this emulsion system, the biocatalyst spontaneously anchors at the oil–water interface, forming a dense, high activity monolayer that optimally orients the enzyme for substrate interaction. When applied to the model reaction of enzymatic degumming of rapeseed oil, the system achieved a degumming efficiency of 96.6% and a 99% oil recovery. At the same time, the residual phosphorus content after degumming in this system was 1.8–2.3 times lower than that of free PLB and homogeneous carrier immobilized enzyme. Furthermore, it exhibited excellent reusability and achieved a 96% degumming efficiency after five consecutive cycles. This work elucidates the rational design of amphiphilic Janus materials for efficient interfacial biocatalysis in oil-processing applications.
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2026
·
IF 3.5
Long Neng-Bing,Zhu Hong-Lin,Li Tian-Hua,Zhang Rui-Feng
A large-sized 3D microflow support was prepared by chemical liquid deposition of an epoxy-based porous polymer within a 3D melamine sponge. This novel support demonstrated advantages over traditional microfluidic reactors in terms of easier scalability, simplified enzyme loading, and superior multiphase dispersion. The resultant composite sponge was utilized as a microflow support for the immobilization of phospholipase D (PLD) through an adsorption and cross-linking method. Under optimal immobilization conditions, the loading capacity and specific activity of the immobilized PLD were 50.2 mg/g support and 14.5 U/mg protein, respectively. The PLD-immobilized microflow supports were positioned in a columnar reactor, where a biphasic transesterification reaction was conducted in a continuous circulation mode powered by two pumps. The PLD-catalyzed reaction yielded phosphatidylserine (PS) with high yields and reaction ratios. Under optimized conditions, the PS yield reached as high as 93.1% within 4 h at 45 °C, by using free PLD the PS yield reached only 61.5% within 12 h. After 10 cycles, the immobilized PLD retained 76% of its original activity.
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xin-Yu You,Wei-Ming Yin,Yan Wang,Chen Wang,Wen-Xiu Zheng,Yuan-Ru Guo,Shujun Li,Qing-Jiang Pan
Heavy metal ions (HMIs) have been widely applied in various industries because of their excellent physicochemical properties. However, their discharging without appropriate treatment brought about serious pollution problems. So it is desirable but challenging to rapidly and completely clean up these toxic pollutants from water, especially utilizing environmentally friendly and naturally rich biomass materials. In this work, we prepared nanocellulose/carbon dots/magnesium hydroxide (CCMg) ternary composite using cotton via a simple hydrothermal method . The removal mechanism towards Cd 2+ and Cu 2+ was investigated using a combination of experimental techniques and density functional theory calculations. CCMg shows a good ability to remove HMIs. It is realized that the interaction between each component of CCMg and cadmium nitrate is mainly of hydrogen/dative bonds. Cadmium nitrate is preferentially enriched by the Mg(OH) 2 moiety, proved by calculated thermodynamics, interfacial interactions and charges. After transformation, the cadmium carbonate precipitate is fixed on the surface by nanocellulose (NC) via chemical coupling; and of interest is that copper ion precipitates in the form of basic sulfate. Due to its high adsorption effect and simple recovery operation, CCMg is having a wide range of application prospects as a water treatment agent.
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DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Guilin Wei,Shuaipeng Chen,Fen Luo,Xiaoyan Shu,Mingfen Wen,Yuexiang Lu,Wenhong Han,Jing Chen,Haijiao Xie,Faqin Dong,Xirui Lu
Nuclear waste immobilization is one of the ways to alleviate the harm of radioactive waste produced in the nuclear field to human beings and the environment. In the current study, granite was utilized as a substrate material for the immobilization of simulated radioactive waste. Based on the obtained results, the relationship between the waste chemical species and the physicochemical properties , such as solid-solubility of waste, infrared, density and leaching data, was systematically studied as well. The findings indicate that the granite substrate used for the immobilization of the simulated waste (Nd 2 O 3 ) exhibits a solid-solubility limit of 27 wt%. When the added content of waste is below 27 wt%, all Nd elements are immobilized into the glassy phase . However, when the waste content exceeds 27 wt%, in addition to being immobilized in the glassy phase, the presence of Nd 2 O 3 reacted with Ca 3 Si 3 O 9 to form Ca 2 Nd 8 (SiO 4 ) 6 O 2 . As the Nd 2 O 3 content increased from 20 wt% to 25 wt%, the microstructures related to Si-, Al-, and Fe-are more involved in the immobilization of waste. However, the appearance of Ca 2 Nd 8 (SiO 4 ) 6 O 2 and Nd 2 O 3 makes the density and Vickers hardness of the matrix not increase further. The maximum density and hardness values are 3.17 g cm −3 and 7.88 GPa, respectively. The leaching results show that the cured body has good anti-leaching performance ( L R Nd = ∼10 −6 g cm −2 d −1 ). Finally, the comparison of vacancy formation energy of Nd in Nd 2 O 3 and Ca 2 Nd 8 (SiO 4 ) 6 O 2 proved that the formation of Ca 2 Nd 8 (SiO 4 ) 6 O 2 is beneficial to the immobilization of simulated waste. This study provides a certain reference for the selection of nuclear waste solidification substrates. This kind of granite material from nature has the potential advantages of low cost and better immobilization effect on waste, and may be more suitable for the treatment of radioactive waste in the future.
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 3.9
Chang Li,Zixuan Li,Yan Sun
Laccase , a green biocatalyst , has been widely used in the field of biodegradation with the assistance of electron mediators. Herein, a novel strategy, polyethyleneimine (PEI)-induced biomimetic mineralization , was explored and applied to co-immobilize laccase and 2,2′-azino bis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS). With the PEI doping, calcium phosphate (CaP) could facilely mineralize within 20 min to form PEI@CaP; ABTS could be co-mineralized with PEI to form (PEI + ABTS)@CaP. Laccase, a negatively charged protein, was then immobilized via electrostatic adsorption onto the embedded PEI in the carriers. Both of the immobilized laccase preparations, denoted as Lac-PEI@CaP and Lac-(PEI + ABTS)@CaP, showed high activity recoveries (96.4 % and 88.0 %, respectively), and increased pH tolerance and storage stability as compared to free laccase. ABTS and laccase loading densities of Lac-(PEI + ABTS)@CaP reached 0.83 mmol g −1 and 100 mg g −1 , respectively. Lac-(PEI + ABTS)@CaP presented higher malachite green (MG) degradation capability than free laccase and Lac-PEI@CaP with free ABTS, possibly due to the proximity effect of the co-immobilized laccase and ABTS. Moreover, Lac-(PEI + ABTS)@CaP exhibited reusability and kept 83.2 % of MG degradation activity after three recycles and 37.9 % in the sixth. This work provided a new strategy for the co-immobilization of laccase and electron mediator, and demonstrated the potential of the biocatalyst for organic pollutant removal.
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高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2023
·
IF 8.7
Shuangchi Li,Ning Wang,Shuiqing Chen,Yuqing Sun,Puwang Li,Jinfang Tan,Xiaoqian Jiang
Phosphate fertilizers have been excessively applied in agricultural production, bringing the risk of phosphorus (P) loss to nearby river systems and low utilization efficiency. In this study, eggshell-modified biochars prepared by pyrolysis of eggshell and corn straw or pomelo peel were applied to soil for enhancing P immobilization and utilization. The structure and properties of modified biochars before and after P adsorption were analyzed using the Brunauer-Emmett-Teller (BET) nitrogen adsorption method, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and scanning electron microscope (SEM). The eggshell-modified biochar performed an excellent adsorption performance for P (up to 200 mg/g), which was well described by the Langmuir model (R 2 > 0.969), showing monolayer chemical adsorption with homogenous surface. The Ca(OH) 2 appeared on the surface of eggshell modified biochars and changed to Ca 5 (PO 4 ) 3 (OH) and CaHPO 4 (H 2 O) 2 after P adsorption. The release of immobilized P by modified biochar increased with decreased pH. In addition, pot experiments of soybean indicated that the combined application of modified biochar and P fertilizer significantly increased the content of microbial biomass P in soil, raising from 4.18 mg/kg (control group) to 51.6–61.8 mg/kg (treatment group), and plants height increased by 13.8–26.7%. Column leaching experiments showed that P concentration in the leachate decreased by 97.9% with the modified biochar application. This research provides a new perspective that the eggshell-modified biochar could serve as a potential soil amendment for enhancing P immobilization and utilization.
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PubMed
高影响因子
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