Food Bioscience
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2025
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IF 5.9
Yushun Chen,Songyu Wang,Kefan Ouyang,Qin Zhang,Xiaojuan Dai,Xing Hu,Qiang Zhao
Potato protein isolate (PPI) is a major byproduct of potato starch processing, and its effective utilization is of great significance in reducing waste, conserving resources, and promoting environmental sustainability. However, the functional characteristics of PPI, such as solubility and emulsifying properties, are insufficient. This study explores a novel enzymatic strategy that utilizes laccase catalysis to covalently couple PPI with polyphenols (resveratrol, luteolin), aiming to simultaneously enhance the functional properties and antioxidant capacity of PPI. This modification increases the solubility of PPI in the critical acidic pH range (3–5); At low polyphenol levels, the emulsifying activity index increased by 12.4–35.4 %. Importantly, covalent binding of polyphenols provides synergistic antioxidant protection, effectively reducing the levels of protein oxidation markers induced by hydroxyl radicals. This study successfully demonstrated that laccase mediated covalent coupling of polyphenols is an effective means of converting PPI into components with enhanced functionality and strong antioxidant activity, providing an important foundation and promising solution for its application as antioxidants in protein based health food systems.
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高影响因子
JOURNAL OF FOOD ENGINEERING
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2018
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IF 3.197
Zhaoliang Wu,Na Li,Xin Zhang,Yanyan Xu,Ting Shu,Wei Liu,Bin Hu
Resveratrol (2,5,4′-trihydroxystilbene) is a naturally occurring phytoalexin produced by some spermatogphytes and it has recently attracted the increasing attention of agronomists and pharmacologists owing to its satisfactory bioactivities. The objective of this work was to develop a cost-effective technology of foam fractionation to recover trans -resveratrol ( trans -Res) from the leaching solution of muscat grape pomace on the assistance of molecular imprinting modified SiO 2 nanoparticles . First, trans -Res was leached from muscat grape pomace through the method of alkaline extraction. Under the suitable leaching conditions, the leaching yield of trans -Res reached 84.34%. Adsorption experiments were performed for describing the adsorption behavior of trans -Res on the molecular imprinting modified SiO 2 nanoparticles . Results indicated that their adsorption could be fairly described by Freundlich model and fitted well with the pseudo-second-order kinetics model. Moreover, the molecular imprinting modified SiO 2 nanoparticles could effectively maintain the stability of trans -Res through avoiding the cis-trans photo-isomerization and oxidative degradation. Finally, foam fractionation was carried out for recovering trans -Res from the leaching solution, in which the concentration of trans -Res was as high as 29.09 mg/L. The molecular imprinting modified SiO 2 nanoparticles were used as the collector. Under the suitable conditions of loading liquid volume 250 mL, particle size of molecular imprinting modified SiO 2 nanoparticles 436.0 nm, sapindus saponin concentration 0.075 g/L, volumetric gas flow rate 100 mL/min and pore diameter of gas distributor 0.180 mm, the enrichment ratio and the recovery percentage of trans -Res reached 13.68 ± 1.03 and 89.73 ± 3.57%, respectively. This work is expected to provide a new technology for selectively recovering non-surface-active materials from the solution with their isomers or analogues, and to facilitate the industrialization of foam fractionation.
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Asian Journal of Pharmaceutical Sciences
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2015
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IF 0
Jian Li,Xiaoqing Miao,Tongkai Chen,Defang Ouyang,Ying Zheng
With hydrophilic surface and high surface area, porous silica has been applied to load insoluble drugs. Compared to solvent equilibrium method, resveratrol (RES)–mesoporous silica microparticles (MSM) solid dispersion prepared by fluid bed demonstrated higher drug loading and more complete dissolution. Pore volume and diameter have more remarkable effects than surface area to the drug loading and in vitro dissolution profiles. RES–polyethylene glycol solid dispersion with high drug loading showed fast but incomplete dissolution due to the recrystallization. The combination of fluid bed and MSM was an effective strategy to improve drug loading as well as dissolution for poorly water-soluble drugs.
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Food Packaging and Shelf Life
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2025
·
IF 10.6
Yanyan Wang,Ruoran Qin,Jiayi Xue,Hui Li,Longwei Jiang
Succinate cyclodextrin/chitosan nanoparticles (SCC NPs) loaded with resveratrol (Res) were successfully synthesized by self-assembly. Nanocomposite films were prepared by adding R-SCC NPs to sodium alginate (SA). The structure, physical and chemical properties, and antioxidant, antibacterial and biodegradability properties of the nanocomposite films were characterized. Scanning electron microscopy (SEM) results revealed that R-SCC NPs in 1–6 % were uniformly distributed in the SA film matrix. FTIR analysis revealed that the R-SCC NPs formed intermolecular hydrogen bonds with SA. The maximum tensile strength of the nanocomposite film was 44.24 ± 0.82 MPa, which was 153.50 % of that of the SA film. The addition of R-SCC NPs markedly enhanced the UV absorption capacity, antioxidant stability, and antibacterial activity of the nanocomposite films. In addition, because Res was encapsulated in the SCC NPs, control over the release from the composite film was achieved. This film has excellent biodegradability in soil and can regulate the soil microbial community. Furthermore, the developed film package can effectively prolong the expiry date of blueberries, so the SA film containing R-SCC NPs as active packaging has significant potential for maintaining food quality.
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International Journal of Nanomedicine
·
2017
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IF 4.3
Jifu Hao,Tiantian Tong,Kai Jin,Qiannan Zhuang,Te Han,Yanping Bi,Jianzhu Wang,Xiaodan Wang
A folic acid (FA)-functionalized drug vehicle platform based on Pluronic 127 (P127)/D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) mixed micelles was orchestrated for an effective delivery of the model drug resveratrol in order to address the problem of poor water solubility and rapid metabolism of resveratrol and improve its targeted accumulation at tumor site. The FA-decorated mixed micelles were prepared using thin-film hydration method and optimized by central composite design approach. The micelles were also characterized in terms of size and morphology, drug entrapment efficiency and in vitro release profile. In addition, the cytotoxicity and cell uptake of the micelles were evaluated in folate receptor-overexpressing MCF-7 cell line. In vivo pharmacokinetic and biodistribution studies were also performed. The average size of the micelles was ~20 nm with a spherical shape and high encapsulation efficiency (99.67%). The results of fluorescence microscopy confirmed the targeting capability of FA-conjugated micelles in MCF-7 cells. FA-modified micelles exhibited superior pharmacokinetics in comparison with that of solution. Further, the low accumulation of resveratrol-loaded FA micelles formulation in the heart and kidney avoided toxicity of these vital organs. It could be concluded that folate-modified P127/TPGS mixed micelles might serve as a potential delivery platform for resveratrol.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
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IF 8
Yiting Zhang,Bing Li,Qianying Ding,Xiaoli Liu,Jiaxuan Ma,Xuan Yao,Zhijun Xia,Yuanyuan Liu,Yongguo Jin
Compared with natural proteins, protein microgels better enhance the performance and stability of high internal phase Pickering emulsions (HIPPEs), with promising food industry applications. In this study, ovalbumin-resveratrol (OVA-RES) composite microgel particles were synthesized via a top-down approach to fabricate HIPPEs, which showed significant improvements in viscoelasticity, storage, centrifugal, and thermal stability. To clarify the stabilization mechanism, the morphology, structure, and physicochemical properties of composite microgels under different RES concentrations were characterized. The results revealed that the optimal performance was attained at a RES concentration of 0.12 mM: at this concentration, RES induced a conformational transition of OVA primarily through hydrophobic interactions. Specifically, the α-helix content decreased from 23.65 % to 12.60 %, the β-sheet content increased from 36.55 % to 49.20 %, and the free sulfhydryl group content rose from 19.12 μmol/g to 21.57 μmol/g. Furthermore, the surface activity of the composite microgels was significantly enhanced, as evidenced by increased hydrophobicity, reduced surface tension, and an elevation in contact angle from 53.6° to 72.85°. In the present study, the emulsifying properties of OVA microgel particles were successfully enhanced through the incorporation of RES, providing new insights into natural protein-polyphenol composite microgels as efficient emulsifiers. Meanwhile, this work develops a feasible food-grade emulsion stabilization and delivery platform for the incorporation of RES into emulsion-based food systems. Future research will further focus on the bioactive functions of OVA-RES composite microgels in food systems, as well as their specific applications and industrialization potential in the food sector.
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Biomedicines
·
2021
·Vol. 9
·期 3
·271
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IF 6.081
Qingjun Kong,Qingzhi Zeng,Jia Yu,Hongxi Xiao,Jun Lu,Xueyan Ren
Resveratrol dimers have been extensively reported on due to their antioxidative activity. Previous studies revealed that resveratrol dimer has been shown to selectively quench singlet oxygen (1O2), and could protect DNA from oxidative damage. The mechanism of resveratrol dimers protecting DNA against oxidative damage is still not clear. Therefore, in this project, the reactants and products of resveratrol dimers protecting guanine from oxidative damage were qualitatively monitored and quantitatively analyzed by UHPLC-QTOF-MS2and UHPLC-QQQ-MS2. Results showed that when guanine and resveratrol dimers were attacked by1O2, mostly resveratrol dimers were oxidized, which protected guanine from oxidation. Resveratrol dimers’ oxidation products were identified and quantified atm/z467.1134 [M-H]−and 467.1118 [M-H]−, respectively. The resorcinol of resveratrol dimers reacted with singlet oxygen to produce p-benzoquinone, protecting guanine from1O2damage. Therefore, it is hereby reported for the first time that the resorcinol ring is the characteristic structure in stilbenes inhibiting1O2induced-DNA damage, which provides a theoretical basis for preventing and treating DNA damage-mediated diseases.Keywords:resveratrol dimers;antioxidative activity;singlet oxygen quenching;DNA damage
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Wang Lihang,Liu Botao,Lu Tingsheng,Luo Chunshan,Li Zhongzhe,Lin Yunfeng
Ankylosing spondylitis (AS) is a chronic systemic inflammatory disease affecting the spine, sacroiliac and peripheral joints, with TNF-α as a core pro-inflammatory cytokine. Macrophages, as major TNF-α producers, form a positive feedback loop that amplifies local inflammatory responses. The use of clinical TNF inhibitors such as infliximab is limited by their high cost and high recurrence rate. This study constructs a targeted nano-delivery system named RSV@tFNAs-TNFαapt, by conjugating TNF-α aptamer to tetrahedral DNA nanostructures and loading resveratrol, a natural polyphenol with potent anti-inflammatory, antioxidant and immunomodulatory activities. The nanosystem showed uniform morphology, good stability and high cell uptake efficiency. In vitro, it promotes M2 macrophage polarization, inhibits pro-inflammatory and tissue-destruction-related factors, and reduced ROS production in synovial fibroblasts. In an AS rat model, it improves motor function, relieves joint swelling, alleviates spinal inflammation and structural damage, and reduces the production of peripheral inflammatory factors. Histopathology results confirms less inflammatory cell infiltration and protected cartilage and bone structure. In summary, RSV@tFNAs-TNFαapt exerts significant targeted anti-inflammatory and joint-protective effects, providing a complementary therapeutic strategy for AS, especially for peripheral joint lesions. Graphical abstract
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Frontiers in Nutrition
·
2022
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IF 6.59
Zhao Yi,Huang Yujian,Gao Kaiguo,Wen Xiaolu,Hu Shenglan,Wang Li,Jiang Zongyong,Xiao Hao
Background: Resveratrol has numerous beneficial properties, including antioxidant, anti-inflammatory, and immunomodulatory properties. High summer temperatures in Southern China affect the reproductive performance of sows. The present study aimed to investigate the effects of dietary resveratrol supplementation in different thermal environments on the reproductive performance, antioxidant capacity, immune function, and intestinal microbes of sows and piglets during late gestation and lactation, as well as their relationship with colostrum immunoglobulin.Methods: A two-phase experiment was conducted with 40 healthy multiparous sows. In the first phase of the experiment, 20 sows were used in a moderate temperature environment, and in the second phase of the experiment, the remaining 20 sows were used in a high-temperature environment. In both phases, sows were fed either a control diet or a diet consists of control diet and 300 mg/kg resveratrol starting on day 75 of gestation. Plasma, milk, and fecal samples were collected to obtain the indices of antioxidant capacity, immune function, and intestinal microbes.Results: The results showed that resveratrol supplementation increased the number of live births by 13.24 and 26.79% in the first and second phases, respectively, compared with the control group. In the second phase, resveratrol supplementation increased litter weight at weaning and in the concentrations of growth hormone (GH), insulin (INS), progesterone (PROG), triglycerides, and uric acid (UA). The plasma superoxide dismutase (SOD) level on day 110 of gestation and day 14 of lactation, as well as glutathione peroxidase (GSH-Px) on day 14 of lactation in the first phase, showed an increasing trend (p = 0.0728, p = 0.0932, and p = 0.067, respectively) in the resveratrol group, compared with the control group. On day 14 of lactation, the plasma total antioxidant capability (T-AOC) level was higher in the second phase, while the plasma malondialdehyde (MDA) level was lower in both phases in the resveratrol group. Resveratrol supplementation increased the abundance of immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) in colostrum and the relative abundance of Lactobacillus and Alloprevotella but decreased the relative abundance of Escherichia-shigella in piglet feces in the second phase. In addition, Spearman's correlation analysis indicated that the weight gain of weaned piglets was positively (p < 0.05) associated with IgM content in colostrum and the abundance of Lactobacillus in the fecal microbiota of piglets in the second phase. Moreover, the abundance of Alloprevotella was positively correlated with the contents of IgA and IgG in colostrum, while the abundance of Lactobacillus was positively correlated with IgM content.Conclusion: These findings indicated that maternal resveratrol supplementation could enhance the growth performance, antioxidant capacity, and intestinal health of piglets in a high temperature environment, which might be associated with increased immunoglobin secretion from colostrum.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·Vol. 24
·期 1
·382
·
IF 15
Jiang Zhou,Li Yuning
Lung cancer (LC) remains a leading cause of cancer-related mortality worldwide, and the limited efficacy of immunotherapy due to treatment resistance underscores the urgent need for new therapeutic strategies. In the present study, CD93-targeted poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating resveratrol (CD93-NPs@RSV) were developed to remodel the metabolic fitness of CD8 + tumor-infiltrating lymphocytes. The nanoparticles were precisely engineered and characterized using dynamic light scattering, transmission electron microscopy, and in vivo imaging, which confirmed their stability and tumor-targeting capability. Mechanistic studies revealed that CD93-NPs@RSV suppressed CD93 expression, facilitated apoptosis-inducing factor (AIF) mitochondrial translocation, and activated oxidative phosphorylation (OXPHOS), thereby enhancing T cell function in the tumor microenvironment. Transcriptomic and proteomic analyses further confirmed regulation of the CD93-AKT-PAK5-AIF signaling axis. In a Lewis LC model, CD93-NPs@RSV significantly inhibited tumor progression and displayed strong synergy with anti-PD-1 therapy, resulting in improved survival outcomes. Collectively, our study demonstrates that CD93-NPs@RSV provide a powerful nanotechnology-driven approach to reverse immunotherapy resistance by reprogramming T cell metabolism. These findings establish a promising paradigm for precision cancer immunotherapy and underscore the translational potential of targeted nanomedicine in overcoming therapeutic bottlenecks in LC. Graphical abstract The alternative text for this image may have been generated using AI.
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Animal Cells and Systems
·
2023
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IF 2.9
Weiming Yan,Yan Sun,Yutong Wang,Wangjiao Liang,Yuxin Xia,Weihua Yan,Meizhu Chen,Tao Chen,Dongliang Li
Upregulation of Sirtuin Type 1 (SIRT1), a nicotinamide adeninedinucleotide (NAD+)-dependent deacetylase, has been proved to protect against ample ocular diseases, while its effect on retinitis pigmentosa (RP) has not been illustrated. The study was aimed to explore the impacts of resveratrol (RSV), a SIRT1 activator, on the photoreceptor degeneration in a rat model of RP induced by N-methyl-N-nitrosourea (MNU), an alkylation. The rats were induced RP phenotypes via the intraperitoneal injection of MNU. The electroretinogram was conducted and revealed that RSV could not prevent the decline of retinal function in the RP rats. The optical coherence tomography (OCT) and the retinal histological examination were performed and showed that the reduced thickness of the outer nuclear layer (ONL) was not preserved by RSV intervention. The immunostaining technique was applied. Afther the MNU administration, the number of the apoptotic photoreceptors in the ONL throughout the retinasand the number of microglia cells present among the outer part throughout the retinas were not significantly reduced by RSV. Western blotting was also performed. The data showed that the level of SIRT1 protein was decreased after MNU administration, while RSV was not able to obviously alleviate the downregulation. Our data together indicated that RSV was not able to rescue the photoreceptor degeneration in the MNU-induced RP rats, which might be due to the MNU-induced consumption of the NAD+.
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MOLECULAR NEUROBIOLOGY
·
2025
·Vol. 63
·期 1
·1-14
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IF 4.3
Cai Minghui,Guo Qi,Li Jiaxin,Dai Xiaoqi,Su Huiyu,Zhang Wenwen,Qiang Na,Li Geng,Jin Zihan,Zhu Hui
The factors influencing hippocampal aging are complex, and it remains challenging to identify effective anti-aging drugs and reveal pharmacological mechanisms. This study investigates the effects of autophagy mediated by the SIRT1/FoxO1 signaling pathway on resveratrol resistance to H 2 O 2 -induced aging and apoptosis in mouse hippocampal neurons. HT22 cells were treated with resveratrol and H 2 O 2 to evaluate the effects of resveratrol on H 2 O 2 -induced aging and apoptosis. The levels of P21, P53, BAX, Cleaved Caspase-3, and Bcl-2 were measured to estimate aging and apoptosis. Western blot, immunofluorescence assays, and transmission electron microscopy observation were used to detect the regulation of resveratrol on autophagy. 3-Methyladenine was used to block autophagy, and EX-527 was used to inhibit SIRT1. The changes of autophagy factors and the levels of SIRT1/FoxO1 signaling pathway were assayed. H 2 O 2 treatment induced aging in HT22 cells, manifesting increased levels of P21 and P53 and decreased cell viability. H 2 O 2 treatment aggravated apoptosis, showing increased levels of BAX and Cleaved Caspase-3, decreased levels of Bcl-2, and increased apoptotic cells. Resveratrol alleviated aging and apoptosis in H 2 O 2 -induced HT22 cells. Mechanically, resveratrol activated autophagy by up-regulating the levels of LC3B Ⅱ/Ⅰ and Beclin1 and down-regulating P62 against aging and apoptosis, which was abolished by 3-Methyladenine. Western blot results showed resveratrol activated SIRT1/FoxO1 signaling pathway which was related to regulation of autophagy. EX-527 treatment suggested resveratrol could not activate autophagy when the SIRT1/FoxO1 signaling pathway was blocked. These findings indicated that resveratrol activated autophagy via SIRT1/FoxO1 signaling pathway to protect against H 2 O 2 -induced aging and apoptosis in HT22 cells.
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