Journal of Functional Foods
·
2025
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IF 4
Yan Ma,Zhengnan Jiang,Hai Xie,Jian Wang,Minni Li,Huifang Ge,Wenbin Li,Zhongwen Xie,Daxiang Li,Xueling Gao,Hongyan Wang
Fermented oxidation fresh tea (FOFT) is a unique dark tea produced by a single strain of Eurotium cristatum , and its impact on obesity has not been extensively studied. Here, we investigated the anti-obesity and metabolic benefits of FOFT using a HFD-induced obesity mouse model. The HFD-fed mice or ND-fed mice were orally administered with FOFT for 12 weeks. The results showed that FOFT invention significantly suppressed body weight gain, ameliorated liver and adipocyte morphology, and improved glucolipid metabolism and intestinal homeostasis imbalance in HFD-fed mice. Notably, FOFT administration caused no adverse effects in ND mice, indicating its safety at tested doses. Moreover, FOFT restored intestinal microbiome dysbiosis and elevated the short-chain fatty acid levels. This gut microbiome shift was associated with reduced systemic inflammation and attenuated hepatic lipid accumulation. These findings suggest that FOFT, as a novel functional food, holds great promise for resistance to obesity and its comorbidities.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jialu Zhang,Yu Fu,Peng Chen,Bowen Liu,Cuiling Ren
Salicylaldehyde is a significant fine chemical product utilized in the flavoring of various foods, excessive intake of salicylaldehyde is detrimental to human health. Its accurate quantitative detection holds considerable importance. Compared with “turn-off” probes, the “turn-on”one allow for more sensitive detection. New silicon nanoparticles (D-SiNPs) which can be used to detect salicylaldehyde by “turn-on” mode were prepared in this work. This D-SiNPs demonstrates highly stability, selectivity and sensitivity. Within the concentration range of 5–50 μM, salicylaldehyde concentration exhibits a strong linear relationship with fluorescence intensity, the limit of detection is calculated to be 0.97 μM. It can be applied to detect salicylaldehyde in baked goods and beverages, achieving good recoveries. These results are highly satisfactory. The detect mechanism was investigated systemetically, the results illustrates this detection is based on Schiff reaction between the amino groups of silicon nanoparticles and aldehyde group of salicylaldehyde, which satisfies the ESIPT mechanism.
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Journal of Environmental Sciences
·
2025
·
IF 6.3
Qingmuke Su,Yaguang Du,Dongyun Du,Tian C. Zhang
Ciprofloxacin (CIP), as a quinolone antibiotic, has broad-spectrum antibacterial properties and can affect methanogenic performance in anaerobic digestion (AD). While previous studies focused on synthetic wastewater, the fate of CIP in real distillery wastewater (RDW) and its impact on microbial adaptation mechanisms remain unclear (such as biotransformation pathways, population dynamics, and the enzymes involved) in RDW is largely unclear. In this study, we investigated AD performance, metabolic pathways, and antibiotic resistance gene (ARG) dynamics using real wastewater spiked with CIP (0.3–2 mg/L). Results indicate that 0.5 mg/L CIP (631.83 mL CH 4 /g·VS) enhanced the methane yield by 6.67 % (592.34 mL CH 4 /g·VS in control), correlating with upregulated enzyme in glycosis, TCA cycle, and methanogenesis (F 420 increased). With full use of short-chain acids, transient volatile fatty acid (VFA) inhibition (≤ 50 mg/L on Day 3) was overcome by Day 10. Metagenomics revealed CIP promoted the production of stress proteins (e.g., cysteine synthase activity doubled). Furthermore, CIP (0.3–1 mg/L) suppressed mobile genetic elements (MGEs) encoding horizontal gene transfer, including isfinder (15.15 %) and integrases (6.25 %), while ARG and virulence factor abundances remained unchanged versus control. This study firstly shows that low-dose CIP in RDW increases methanogenesis via metabolic adaptation without exacerbating ARG risks. MGE suppression implies that CIP may lessen the possibility of ARG diffusion in AD systems. These results offer vital information for improving AD performance in the treatment of wastewater contaminated by antibiotics and developing methods to strike a balance between antibiotic removal and ARG control.
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CARBOHYDRATE POLYMERS
·
2025
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IF 12.5
Yi Zhang,Lingjin Li,Yahui Gao,Li Cheng,Yan Hong,Zhengbiao Gu
Butyrylated normal maize starch (BNMS) is a butyrate-carrying and resistant starch (RS). BNMS is fermented by the gut microbiota to produce large amounts of short-chain fatty acids (SCFAs), particularly butyrate, which are beneficial for intestinal health. However, the effects of BNMS on gut microbiota and metabolites, and its advantages over exogenous butyrate and RS, are unclear. The specific effects of BNMS were investigated via in vitro fermentation using sodium butyrate (SB) and high-amylose maize starch (HAMS) as controls. Compared to SB and HAMS, BNMS enhanced gas production, lowered pH, and produced numerous SCFAs. Additionally, BNMS effectively stimulated beneficial bacteria proliferation, including Coprococcus , Faecalibacterium , Megamonas , Collinsella , Blautia , and Bifidobacterium , and suppressed harmful bacteria proliferation, including Escherichia-shigella and Desulfovibrio , resulting in a healthier colony structure. Similarly, BNMS modulated many amino acids, neurotransmitters, vitamins, sphingolipids, carbohydrates, hormones levels, and multiple metabolic pathways, including arginine and proline metabolism, tryptophan metabolism, synaptic vesicle cycle, sphingolipid metabolism, riboflavin metabolism, amino sugar and nucleotide sugar metabolism, and steroid hormone biosynthesis. Overall, BNMS positively affected gut microbiota and metabolites, exhibiting advantages over exogenous butyrate and RS. These results provide some theoretical basis for the BNMS development and application as a functional food.
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JOURNAL OF ETHNOPHARMACOLOGY
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2025
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IF 5.4
Xinglishang He,Wanfeng Xu,Linzi Li,Xiaofeng Jiang,Yingjie Dong,Kun Liu,Xiaojie Feng,Jie Su,Bo Li,Guiyuan Lv,Suhong Chen
Ethnopharmacological relevance Polygonatum Rhizoma Ginseng Formula (HJRS) is a traditional Chinese medicine preparation commonly used to enhance immunity. Traditionally, it is believed to “moisten dryness and nourish yin, tonify the spleen, and benefit qi.” However, a systematic evaluation of its efficacy and mechanism of action is still lacking. Aim of the study To explore the pharmacodynamic effects and underlying mechanisms of HJRS in cyclophosphamide (CTX)-induced immunocompromised model mice. Method To explore the immunomodulatory effects of HJRS, we used cyberpharmacology combined with in vivo experiments. An immunocompromised mouse model was established using CTX, and different doses of HJRS were administered by gavage during the modeling period. The efficacy of HJRS was evaluated by assessing immune-related parameters, including body weight, heat pain threshold, and behavioral activity. Biochemical markers (acid phosphatase, total nitric oxide synthase, and lactate dehydrogenase), immune function indicators (C3, C4, secretory immunoglobulin A [sIgA]), and immune organ indices (spleen and thymus coefficients) were measured. Additionally, intestinal mucosal immunity (Peyer’s patches, sIgA, and IgM), cellular immunity (delayed-type hypersensitivity [DTH]), and humoral immunity (serum hemolysin, hemolysin, and IgM) were analyzed in the model mice. To further investigate the underlying mechanisms, we used 16S RNA sequencing, western blotting, gas chromatography-mass spectrometry, and other techniques to examine the intestinal microbiota, the short-chain fatty acids–G protein-coupled receptor 41 (SCFAs-GPR41) pathway, and the downstream mitogen-activated protein kinase (MAPK) signaling pathway. Result Network pharmacology analysis identified 34 active compounds and predicted 154 potential targets in HJRS that may regulate immune function by interacting with multiple targets (tumor-necrosis factor [TNF], PIK3CG, JUN, AKT1, and MAPK) and immune-related signaling pathways (MAPK and TNF-signaling pathway). Experimental validation demonstrated that HJRS alleviated symptoms of weakness and reduced behavioral activity in the model mice. It significantly enhanced the DTH response, elevated serum hemolysin levels, and restored the balance of white blood cells, lymphocytes, red blood cells, and hemoglobin percentages. HJRS also rebalanced the serum CD3 + CD4 + /CD3 + CD8 + ratio and increased C3 and C4 levels. Furthermore, it improved immune organ function by raising the thymus and spleen coefficients and increasing serum IgA, IgA, and IgM levels. Histopathological examination revealed that HJRS ameliorated CTX-induced pathological damage in the thymus, spleen, and intestines. HJRS regulated gut microbiota composition by enhancing the richness of Bacteroidetes and increasing the Firmicutes/Bacteroidetes ratio, while reducing the abundance of Firmicutes . It decreased the relative abundance of harmful genera, such as Bacteroides and Enterorhabdus , and promoted the growth of beneficial genera, including Muribaculaceae , Clostridia , and Alloprevotella . Additionally, HJRS increased the fecal acetic acid, propionic acid, hexanoic acid, and total SCFA levels in the model mice. It activated the SCFAs-GPR41 pathway and downstream MAPK signaling, thereby enhancing intestinal mucosal immune function. Conclusion HJRS effectively improved immune function in CTX-induced immunocompromised mice. Its mechanism of action may be associated with the regulation of intestinal microbiota dysbiosis, activation of the intestinal “SCFA-GPR41” pathway, and modulation of the downstream MAPK signaling pathway.
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PHYTOTHERAPY RESEARCH
·
2025
·
IF 6.3
Minghao Fan,Yudi Jiang,Chao Cai,Zhe Wang,Lu Chen,Shumin Hu,Xin Zhang,Hua Yin,Zhonghua Qian,Shuli Huang,Jiachen Yang
Depression often develops in young individuals and is linked to complications like cognitive impairment. Conventional antidepressants show limited efficacy in restoring cognitive function and may cause adverse effects. Green tea, a safe and health-promoting beverage, offers various health benefits. This study investigated the effects of long-term green tea consumption on stress-induced depression-like behavior and mild cognitive impairment in animal models. We established a rodent model of mild depression and studied the effects of green tea on depression-like behavior and cognitive impairment through comprehensive evaluation, including behavioral assessments, neurotransmitter quantification, gene and protein expression analysis, blood metabolite profiling, and gut microbiota characterization. Results demonstrated significant improvements in mood, long-term memory, and sterol and glycerophospholipid metabolism. Green tea repaired the intestinal barrier and upregulated genes vital for tight junctions and mucin production. It also enhanced gut microbiota composition, reducing the Firmicutes-to-Bacteroidetes ratio and promoting beneficial bacteria such as NK4A136 , Muribaculum , and Gordonibacter . These microbiota changes improved liver lipid metabolism and alleviated depressive symptoms. Green tea effectively mitigates depression-like behavior and cognitive deficits by modulating the gut-liver-brain axis.
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Food & Function
·
2025
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IF 5.4
Mengzhen Jia,Xiaorui Wang,Fangjie Ning,Wenxiu Wang,Xinyu Hu,Kexin Geng,Junqing Wen,Shan Wu,Bin Wang,Zhigang Liu
Alzheimer's disease (AD) is mainly manifested by cognitive dysfunction, accompanied by excessive β-amyloid (Aβ) deposition and neuroinflammation. The regulation of vagus nerve (VN) signal transmission is crucial for influencing the pathological progression of AD and exploring new therapeutic approaches. Two doses of 2'-FL (500 &1000 mg/kg) were administered orally to AD model mice. The 2'-FL rescued spatial and recognition memory deficits in AD mice. Moreover, 2'-FL reduced Aβ deposition and neuroinflammation. The 2'-FL enhances c-Fos expression in the NTS. This suggested that 2'-FL alleviated AD related cognition by enhancing VN afferent activity. Vagotomy demonstrated that the alleviation of AD related cognition by 2'-FL depended on the presence of VN. Moreover, 2'-FL enhanced gut barrier function, alleviated gut inflammatory. Notably, 2'-FL also reshaped the gut microbiota composition, increasing the relative abundance of Intestinimonas, Muribaculum, and others. Moreover, 2'-FL promoted the production of SCFAs. Correlation analysis showed that propionate was highly correlated with other related indicators. After vagotomy, although 2'-FL promoted the SCFAs production, it didn't alleviate cognitive impairment. This suggested that the neuroprotective effect of SCFAs could also be partially dependent on the VN. 2'-FL rescued the cognitive deficits in AD mice, which was partly explained by changes in gut microbial composition, production of SCFAs, and the presence of VN.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Yu Zhang,Songyuan Luo,Kai Huang,Jie Liu,Sen Li,Hongdong Song,Hongwei Cao,Xiao Guan
This study systematically evaluated the gastrointestinal fate of pea 7S, 11S globulin, and pea protein isolate (PPI) and their impact on gut using INFOGEST digestion, fecal fermentation, and multiomics approaches. During the gastrointestinal stage, pea proteins were largely digested, while pea 7S globulins and PPI exhibited partial resistance to gastric digestion. Intestinal digestion significantly enhanced Lys bioavailability, although Met remained as the first limiting amino acid across all pea proteins. During in vitro fermentation inoculated with microbiota from hyperuricemic individuals, pea proteins significantly reduced the production of trimethylamine N-oxide (TMAO) and guanosine relative to beef protein. Concurrently, pea protein supplementation promoted the generation of short-chain fatty acids and selectively enriched beneficial populations of gut microbiota such as Phascolarctobacterium and Akkermansia. Spearman correlation revealed that amino acid composition of pea proteins significantly affected microbial composition and gut-produced metabolites. Our results elucidate the structural-digestive-microbial interactions of pea proteins.
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ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Zhiye Yan,Weiwei Tang,Bin Li
Metabolomics is a potent tool used for discovering disease biomarkers and investigating intricate mechanisms of diseases by analyzing metabolite changes in biological systems. However, challenges in metabolite detection have not been fully addressed due to their vast physicochemical diversity and broad concentration range, impacting comprehensive profiling of the metabolome, particularly for certain metabolites with limited detection sensitivity. Therefore, a novel chemical derivatization method based on hydralazine (HZN) was developed for highly sensitive detection of various poorly ionized small metabolites in biological samples using liquid chromatography–tandem mass spectrometry (LC–MS/MS). HZN-based multifunctional derivatization mainly targeted metabolites with carbonyl, carboxyl, and phosphoryl groups, such as free fatty acids, keto-acids, hydroxy acids, bile acids, fatty aldehydes, and phosphoryl compounds. Main parameters affecting the derivatization efficiency were investigated, such as the condensation reagent, and optimal conditions were obtained. The labeling of HZN enabled the generation of characteristic fragment ions through collision-induced dissociation, avoiding the laboriousness of deducing MS/MS fragmentation and facilitating large-scale quantitative analysis. The sensitivities of HZN-derivatized metabolites improved significantly, ranging from 10 to 5000-fold, and the limits of quantitation for derivatized metabolites varied from 5 pM to 100 nM, depending on their structures. Furthermore, alterations of various gut microbiota-derived metabolites in mice fed with a high-fat diet were unraveled using the HZN-based derivatization method, indicating its promising applications in the investigation of disease-related changes in the metabolome.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Xiaojiao Zheng,Yulin Wang,Hongli Zhang,Qingrong Peng,Xianzhao Zhang,Xu Jing,Fanrong Zhao,Jiajun Han
A green, rapid, and efficient homogeneous liquid–liquid microextraction technique involving the use of bio-based solvent 2-methyltetrahydrofuran was developed to extract deltamethrin, beta-cypermethrin, and bifenthrin from medicine–food homology tea samples, including mint-, ginger-, cinnamon- and chrysanthemum-based tea. Various solvents were screened via a conductor-like screening model, and three superior solvents were optimised and validated. 2-Methyltetrahydrofuran was selected as the extractant and added to the sample, and phase separation was induced with ammonium sulfate, achieving extraction in under 1 min without centrifugation. Mechanistic studies using density functional theory calculations and molecular docking revealed that van der Waals forces and hydrogen bonds dominate the interactions. The method exhibited excellent linearity within the range of 0.005–0.5 μg mL −1 and recoveries between 80.6 % and 109.8 %. The environmental sustainability of the entire process was verified. These results emphasise the methold's potential for applications in the trace analysis of pesticides in diverse food matrices .
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Foods
·
2025
·Vol. 14
·期 24
·4272
·
IF 5.1
Mengqi Wang,Yi Zhang,Yunqi Gao,Huanyu Zheng,Mingming Zheng
Ethyl butyrate is a typical flavor ester with pineapple-banana scents, but the poor yield from natural fruits limits its feasibility in food and fragrance industries. In this study, dendritic fibrous nano-silica (DFNS) was hydrophobically modified with octyl groups (DFNS-C 8 ) to immobilize Candida antarctica lipase B (CALB) for solvent-free esterification of ethyl butyrate. The immobilized lipase CALB@DFNS-C 8 , with the enzyme loading of 354.6 mg/g and the enzyme activity of 0.064 U/mg protein, achieved 96.0% ethyl butyrate conversion under the optimum reaction conditions where the molar ratio of butyric acid to ethanol was 1:3, with a reaction temperature and time of 40 °C and 4 h. Under the solvent-free catalytic reactions, CALB@DFNS-C 8 presented the maximum catalytic efficiency of 35.1 mmol/g/h and retained 89% initial activity after ten reuse cycles. In addition, the immobilized lipase can efficiently catalyze the synthesis of various flavor esters (such as butyl acetate, hexyl acetate, butyl butyrate, etc.) and exhibits excellent thermostability and solvent tolerance. A molecular docking simulation reveals that the hydrophobic cavity around the catalytic triad stabilizes the acyl intermediate and ensures the precise orientation of both acid and alcohol substrates. This work provides new insights into the sustainable production of flavor esters using highly active and recyclable immobilized lipases through rational carrier hydrophobization and structural confinement design.
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LANGMUIR
·
2025
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IF 3.9
Song Wei,Xueyong Huang,Xuexiao Chen,Lei Zhang
With their excellent optical properties, perovskite thin films demonstrate vast potential for practical applications, ranging from solid-state lighting to advanced display technique. In this work, environmentally benign ionic liquids were employed to synthesize high-quality CsPbBr3 perovskite films. Several carboxylate amine ionic liquids were introduced to prepare CsPbBr3 perovskite thin films. The optimal preparation conditions of the perovskite thin films were screened and predicted by machine learning algorithms. The predicted results are highly consistent with the subsequent verification experiments. The employment of machine learning algorithms has been demonstrated to result in enhanced PL intensity and improved film quality in perovskite thin films. This work provided a new strategy for the utilization of machine learning algorithms in perovskite luminescent materials.
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危险品化学品经营许可证（带存储）