INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2016
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IF 3.994
Jinli Li,Xudong Cheng,Yan Chen,Weiming He,Li Ni,Peihua Xiong,Minggang Wei
Luteolin (LUT) is an active agent in cancer prevention and a potential candidate for clinical chemotherapy. However, poor water solubility and extensive excretion have limited the clinical use of luteolin. Herein, we attempted to develop vitamin E d-α-tocopherol acid polyethylene glycol 1000 succinate (TPGS) coated liposomes to improve the accumulation of luteolin in lung cancer. Luteolin or coumarin-6 loaded liposomes were prepared using film-dispersion methods and characterized according to their particle size, polydispersity, zeta potential , and drug encapsulation efficiency. Cellular uptake properties and cytotoxicities of luteolin or coumarin-6 loaded liposomes were determined in A549 cells. Optical in vivo imaging was employed as a method of assessing tumor targeting. The antitumor effect was evaluated in mice xenotransplanted with A549 cancer cells. The results showed that TPGS coated liposomes are spherical, and are ∼176.2 nm in size. TPGS coated liposomes enhanced cellular uptake and apoptosis by upregulating the Bax/Bcl-2 ratio, resulting in improved cytotoxicity in A549 cells. TPGS coated liposomes significantly accumulated in tumor tissue and enhanced tumor inhibition without altering the structures of other organs. Thereby, TPGS coated liposomes provide an effective strategy in cancer chemotherapy for model drugs with poor water solubility such as luteolin.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2015
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IF 3.313
Huan-Na Lin,Bin-Bin Hu,Ming-Jun Zhu
Clostridium thermocellum can degrade lignocellulosic materials to produce hydrogen , acetate, lactate and ethanol, and surfactants have been proven to have the ability of increasing the enzymatic hydrolysis of cellulosic substrates. Here, the effects of surfactants on the fermentation of spent mushroom compost by C. thermocellum were investigated and their underlying mechanisms were preliminarily studied. PEG8000 and JFC-E had different effects on the fermentation performance of spent mushroom compost. Under optimum conditions, PEG8000 increased the hydrogen production by 59.78%, compared to the control without surfactant, while JFC-E improved the accumulation of glucose and xylose rather than hydrogen yield. PEG8000 also had different impacts on the bioconversion of non-lignin substrates (Avicel, carboxyl methyl cellulose and filter paper). With added PEG8000, the hydrogen production was improved when using Avicel as the substrate, and with PEG8000, the yields of products and sugar accumulation were different from those without PEG8000, indicating that PEG8000 may increase the hydrogen yield by shifting the microbial metabolism.
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CHEMOSPHERE
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2024
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IF 8.1
Qiqi Zhang,Leping Chu,Qin Yang,Wenqing Wo,Anlin Xu,Yide He,Yongjun Zhang
E-peroxone process is an emerging electrochemical oxidation process , based on ozone and the in-situ cathodic generation of H 2 O 2 , but the stability of cathode is one of the key restraining factors. In this study, we designed a multilayer gas diffusion electrode (GDE) decorated with a commercial hydrophobic membrane for the degradation of pyridine. It was found that a proper control of membrane pore sizes and hot-pressing temperature can significantly promote the GDE stability. Subsequently, key operational parameters of the constructed E-peroxone system were investigated, including the ozone concentration, current density, pH value, electrolyte type and initial concentration of pyridine. The degradation pathways were proposed according to six identified transformation products. The toxicity variation along the degradation progress was evaluated with microbial respiration tests and Toxicity Estimation Software Tool (T.E.S.T.) calculation and an efficient detoxification capacity of E-peroxone was observed. This research provides a theoretical basis and technical support for the development of highly efficient and stable E-peroxone system for the elimination of toxic organic contaminants.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Lin Wang,Yu Pang,Meihua Xin,Mingchun Li,Lulu Shi,Yangfan Mao
Chitosan exhibits good biocompatibility and some antibacterial activity , making it a popular choice in biomedicine, personal care products, and food packaging. Despite its advantages, the limited antibacterial effectiveness of chitosan hinders its widespread use. Introducing a six-membered heterocyclic structure through chemical modification can significantly enhance its antimicrobial properties and broaden its potential applications. In order to explore the effect of six-membered heterocyclic structure on the antibacterial and antibiofilm activities of chitosan. In this study, seven chitosan derivatives containing six-membered heterocyclics were prepared. They were characterized using Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and elemental analysis . Cell viability assay showed that they were non-toxic. The antibacterial and antibiofilm activities against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) were evaluated. Our research findings demonstrate that increasing the hydrophobicity , alkalinity and charge density of the substitute groups improved the antibacterial and antibiofilm activities of chitosan. This study also offers valuable insights into the quantitative structure-activity relationships of chitosan derivatives in terms of antibacterial and antibiofilm activities.
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Foods
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2024
·Vol. 13
·期 12
·1865
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IF 4.7
Jiayu Lu,Zheng Jiang,Jingjie Dang,Dishuai Li,Daixin Yu,Cheng Qu,Qinan Wu
Chamomile has become one of the world’s most popular herbal teas due to its unique properties. Chamomile is widely used in dietary supplements, cosmetics, and herbal products. This study aimed to investigate the volatile aromatic components in chamomile. Two analytical techniques, gas chromatography–mass spectrometry (GC-MS) and an ultra-fast gas chromatography electronic nose, were employed to examine samples from Xinjiang (XJ), Shandong (SD), and Hebei (HB) in China, and imported samples from Germany (GER). The results revealed that all chamomile samples contained specific sesquiterpene compounds, including α-bisabolol, bisabolol oxide, bisabolone oxide, and chamazulene. Additionally, forty potential aroma components were identified by the electronic nose. The primary odor components of chamomile were characterized by fruity and spicy notes. The primary differences in the components of chamomile oil were identified as (E)-β-farnesene, chamazulene,α-bisabolol oxide B, spathulenol andα-bisabolone oxide A. Significant differences in aroma compounds included geosmin, butanoic acid, 2-butene, norfuraneol,γ-terpinene. This study demonstrates that GC–MS and the ultra-fast gas chromatography electronic nose can preliminarily distinguish chamomile from different areas, providing a method and guidance for the selection of origin and sensory evaluation of chamomile. The current study is limited by the sample size and it provides preliminary conclusions. Future studies with a larger sample size are warranted to further improve these findings.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Mohamed Eid,Jingsong Zhu,Muhammad Asif Ismail,Bin Li
Chemically cross-linked hydrogel nanoparticles (HGNPs) offer enhanced properties over their physical counterparts, particularly in drug delivery and cell encapsulation. This study applied pH-thermal dual responsive bio-adhesive HGNPs for dual complexation and enhanced the controlled release and bioavailability of cisplatin (CDDP) and Vitamin E (VE) drugs. The CDDP was loaded into the HGNPs via chemical conjugation with the carboxyl groups in the HGNPs surface by soy polysaccharides (SSPS). At the same time, the host-guest interaction complexed the VE through the β-cyclodextrin (β-CD). The HGNPs showed a uniform HGNPs size distribution of 90.77 ± 14.77 nm and 81.425 ± 13.21 nm before and after complexation, respectively. The FTIR, XRD , XPS , and zeta potential confirmed the conjugation. The cumulative release percent of CDDP reached 98 % at pH 1.2, while <45 % was released at pH 7.4. Our HGNPs enhance the incorporation of CDDP by substituting its chlorides with carboxyl groups of the SSPS; the loading of CDDP and VE was 15 ± 0.33 and 11.32 ± 0.25 wt%, respectively. Moreover, the CDDP and VE also released slower from the HGNPs at 25 °C than at 37 °C and 42 °C. The (VE/CDDP)-loaded HGNPs exhibited longer circulation time in vivo than free CDDP and free VE suspension.
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SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Mengyuan Chen,Chong Wang,Jing Tan,Jin Wang,Chen Wang
Nanofluidic membranes hold great prospects in sensitive and rapid detection of bioanalysts, while remaining lacking robust and general approaches for specificity. In this work, we introduce a cell membrane-inspired nanofluidic membrane with excellent ion current rectification properties as a simple and robust platform for label-free and ultrasensitive biosensing. The asymmetric nanofluidic membrane is prepared by coupling a two-dimensional covalent organic framework (COF) membrane to an anodic aluminum oxide (AAO) nanochannel via hydrogen bonding. The prepared COF/AAO membrane presents selective ion transport due to the asymmetric structure and anion selectivity of COF. With the large surface area and abundant functional modification sites of COF, further grafting of the recognition unit can be easily achieved to mimic the recognition process of receptors and ligands on cell membranes. As a proof of concept, the application in bacterial detection is demonstrated by covalently bonded Concanavalin A as recognition units. The highly amplified ionic current based on ion current rectification properties of the COF/AAO membrane allows sensitive and selective bioanalysis. Specifically, the proposed asymmetric nanofluidic membrane presents a linear detection range from 10 to 10 6 CFU/mL with an ultralow detection limit of 7 CFU/mL for E. coli . This work reveals the great potential of COF/AAO hybrid nanofluidic membrane as a sensor for rapid and precise bioanalysis.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Chenlu Zhu,Renjiao Han,Bixuan Gu,Caiyun Wang,Han Liu,Xiaomei Lyu,Jian He,Ruijin Yang
The limited expression of cellobiose 2-epimerase poses a significant constraint on the industrial enzymatic production of lactulose. Extensive modifications to the expression cassette offer a means to enhance the yield of recombinant proteins. In this study, an integrated strategy, combining individual and collaborative approaches, is proposed to fine-tune each stage of the CsCE overexpression program. This strategy involves the multidimensional integration of standardized genetic elements at various levels, including transcription, translation, folding, and three-dimensional structure. The volumetric activity of the final recombinant strain was markedly increased by 12-fold compared to the wild-type strain, reaching 2260.62 U/L. The protein expression in the newly developed high-yield recombinant strain exhibited a significant enhancement, with a higher proportion of soluble protein compared to that of inclusion bodies. Our findings offer insights into the multifaceted synergistic regulation of protein expression processes, holding promising implications for the production of heterologous recombinant proteins.
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Nano Energy
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2024
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IF 16.8
Ya Cheng,Junjie Ning,Ce Wang,Wendong Zhu,Linxi Hou
As development of Internet of Things, the E-skin sensor urgently needs to break through the constraints of sensing reliability, miniaturization, and portability. Occurrence of smart textile of integrated triboelectric nanogenerator, bring the endless possibility for exploring advanced sensor, which possess inimitable lightweight, permeability, flexibility, and washability. Herein, an all-nanofiber Janus textile with multi-layer structure was fabricated by continuously electrospinning and electrospray technologies, acting as a self-powered E-skin sensor. After triboelectric layer was modified by fluorinated polyurethane, the optimal triboelectric output reach 356 V, 2.88 μA, 80.12 nC, and 2.49 W m −2 , which could power small electronics via rectifier circuit. The great sensitivity (6.79 kPa −1 ), operational stability, and air permeability (1010.55 g m −2 •24 h −1 ) was realized. As a proof-of-concept, an intelligent Janus textile-based system was fabricated, the precise control of robotic hand and accurate identification of material were realized with assistance of electric circuit module and deep learning (one-dimensional convolutional neural networks), which would present tactile identification for further intelligent robotics and human-machine interaction (HMI).
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Wenbin Li,Gaocan Qi,Haihui Ding,Weibin Yan,Xijun Liu,Zhihao Yuan
The safe use of fuel gases in industrial production and daily life requires prompt and accurate detection of their concentrations in the environment. However, due to the strong reducing properties of the flammable gases used as fuel, traditional chemoresistive gas sensors face challenges in simultaneously detecting multiple flammable gases. To address this issue, we have developed a metal oxide-based electronic nose capable of detecting common fuel gases, including CH 4 , H 2 , and CO. By controlling the composition and structure of metal oxides, as well as introducing noble metal modifications, we optimized the response differences of these gas sensitive materials to CH 4 , H 2 , and CO. Four sensitive materials were selected from 8 candidates using principal component analysis to construct gas sensor array on a compact four-channel circuit board. The response sensitivities from the sensor array to various concentrations of CH 4 , H 2 , CO, and their mixtures were utilized to train a backpropagation artificial neural network. A new component-identification outlet layer was integrated with the traditional concentration-recognition to improve the training speed and enable simultaneous identification of gas components and concentrations. Through combined material optimization and dual-output training, the backpropagation artificial neural network achieved 90% training accuracy within just 50 iterations, with both sparse cross-entropy loss and mean squared error reduced below 0.005 after 300 training epochs. The resulting E -nose can accurately identify gas components and determine their concentrations in single-, binary-, and ternary-component mixtures of CH 4 , H 2 , and CO, with detection accuracies exceeding 91%. This study provides a practical approach for E -nose construction, enabling reliable monitoring of flammable gases.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Yibin Yang,Rui Chen,Zhiqiang Zhang,Yuke Qin,Attiqa Parveen,He Bai,Xiaoqiang Jia
Phenanthrene, a pervasive polycyclic aromatic hydrocarbon (PAH), poses significant environmental and health risks due to its persistence and bioaccumulation. This study presents the development of a synthetic bacterial consortium comprising three engineered Escherichia coli BL21(DE3) strains, each harboring distinct catabolic modules encoded by pET-28(a) plasmids for the complete mineralization of phenanthrene. The consortium was engineered by integrating 17 genes critical for phenanthrene degradation, organized into three distinct modules that harness the collective metabolic potential of separate strains to enhance the degradation process. Each module was tailored to a specific phase of phenanthrene catabolism, ensuring the coordinated and efficient breakdown of the pollutant. Following the identification of rate-limiting enzymes within the up- and downstream degradation modules, the eight corresponding genes were subjected to laboratory evolution using the SCRaMbLE method to bolster the degradation capabilities of the strains. The consortium was then tested in a minimal salt medium (MSM) containing 100 mg/L phenanthrene. Following their evolution, the two SCRaMbLE strains E. coli SCRPHE033 and E. coli SCRKA065 exhibited enhanced degradation rates of 11.5 and 25.18 % for their respective substrates. Finally, the comprehensive phenanthrene degradation system, which includes the two SCRaMbLE strains and E. coli CRSA, was subjected to comprehensive optimization, after which it achieved a significant degradation efficiency of 84.33 % for 100 mg/L phenanthrene within a 120-hour timeframe. This research demonstrates the potential of synthetic biology in constructing microbial consortia for environmental bioremediation based on the SCRaMbLE method, offering a promising strategy for mitigating PAH pollution.
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FOOD RESEARCH INTERNATIONAL
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2025
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IF 8
Jiaying Jin,Xiaogang Wang,Yu Zhang,Liangjie Ba,Su Xu,Lingshuai Meng,Xiaohong Kou,Sen Cao
Blueberry aroma is a key determinant of fruit quality and market value. However, the mechanisms underlying its formation during ripening remain poorly understood. This study utilized the highbush blueberry cultivar ‘ Legacy ’ and employed an integrated approach combining E -nose, GC–MS, GC-IMS, and transcriptomics to systematically analyze the formation and changes in VOCs as well as the underlying molecular mechanisms throughout fruit ripening. The results revealed that during the entire fruit ripening process, VOCs were predominantly composed of alcohols, aldehydes, and terpenoids. Key compounds, including hexanol, linalool and geraniol, progressively accumulated. Furthermore, the combination of GC–MS and GC-IMS provided a more comprehensive profile of the volatile composition in blueberry fruit. Transcriptomic analysis further elucidated its metabolic regulatory mechanisms: 1) Terpenoid biosynthesis is co-regulated by the MAV and MEP pathways, with the MEP pathway playing a dominant role in monoterpene biosynthesis; 2) Fatty acid metabolism generates C6 aldehydes via LOX2S, while upregulation of ADH gene expression significantly enhances the conversion of aldehydes to alcohols. This study provides valuable theoretical foundations for flavor development during blueberry ripening.
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