CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Guowen Qian,Peirong Fan,Jiandong Ye
Creating interconnected macropores in calcium phosphate cement (CPC) is an effective strategy to promote its degradation and osteogenesis. However, little attention has been given to the osteogenic effect of the CPC scaffolds with pre-forming and in-situ forming interconnected macropores. Herein, two types of CPC scaffolds were prepared by infiltrating CPC pastes into 3D-printed polycaprolactone (PCL) and poly(lactic-co-glycolic acid) (PLGA) networks. Meanwhile, the sacrificial PCL network within CPC was dissolved to obtain the CPC scaffold with approximately 300 μm macropores, whereas the PLGA network was retained within the CPC to obtain the PLGA/CPC scaffold. The results indicated that the PLGA/CPC scaffold showed higher degradation rate and compressive strength compared to the CPC scaffold with pre-forming interconnected macropores. However, the proliferation rate and alkaline phosphatase activity of mouse bone mesenchymal stem cells cultured with the CPC scaffold were superior to those cultured with the PLGA/CPC scaffold. In addition, greater formation of new bone and material degradation in the CPC scaffold compared to the PLGA/CPC scaffold after implanted in the rabbit femoral defects.
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DOI
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Lukuan Li,Zhe Wang,Tong Wang,Nannan Zhou,Fang Qiao,Zhen-Yu Du,Mei-Ling Zhang
Short-chain fatty acids (SCFAs) can enhance the growth of aquatic animals and improve flesh quality through the gut-muscle axis. However, the effects of valeric acid, one of the SCFAs, on aquatic animals remain unreported. This study sought to explore the effects of valeric acid on Nile tilapia ( Oreochromis niloticus ). A total of 360 Juvenile tilapia (mean weight, 1.820 ± 0.007 g) with 3 tanks for each treatment were randomly assigned to 12 rectangular acrylic tanks and fed with four isolipidic (6 % lipid) and isonitrogenous (37 % protein) diets for 60 days, including a control diet (CK) and three experimental diets supplemented with 500 mg/kg (CK + LV), 1000 mg/kg (CK + MV), and 2000 mg/kg (CK + HV) of sodium valerate. The results discovered that the enhanced flesh hardness ( p < 0.01) of tilapia caused by valerate was attributed to decreased myofiber size ( p < 0.001). Transcriptome analysis revealed significant enrichment of the Forkhead box O (FOXO) signaling pathway in the valerate-treated group ( p < 0.001). Subsequent qPCR, western blot, immunohistochemistry, and immunofluorescence analyses demonstrated that valerate inhibited muscle cell proliferation by activating the FOXO signaling pathway, suppressing FOXO nuclear export, and consequently downregulating cyclins and cyclin-dependent kinases (CDKs) ( p < 0.01). Furthermore, valerate suppressed muscle differentiation by inhibiting the transcription and protein levels expression of myogenic regulatory factors (MRFs) ( p < 0.001). Additional validation in C2C12 myoblasts and myotubes confirmed that valerate indeed inhibited myoblasts proliferation and myotubes differentiation ( p < 0.001). These findings indicated that valerate reduced myofiber size by suppressing myoblasts proliferation and myotubes differentiation. This study elucidated the effects and mechanisms of valerate on skeletal muscle and introduced a novel approach to enhance fish flesh hardness.
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DOI
高影响因子
Friction
·
2023
·Vol. 12
·期 3
·474-489
·
IF 6.8
Xu Wenya,Yang Guangbin,Zhang Shengmao,Xu Jun,Zhang Yujuan,Sun Tianhua,Song Ningning,Yu Laigui,Zhang Pingyu
Nickel (Ni) nanoparticles can be enriched on the surface of iron-based frictional pairs, which provides the possibility to get rid of the competitive adsorption between the polar species of vegetable oil and the surface-active nano-additives thereon. In this paper, nickel acetylacetonate was used as a precursor to in-situ synthesize nickel nanoparticles with an average diameter of about 12 nm in rapeseed oil (RO) as the reducing agent, surface modifier, and solvent as well. The tribological properties of the as-synthesized Ni nanoparticles were evaluated with a four-ball tribometer, and their tribomechanism was investigated based on the characterizations of the tribofilm on rubbed steel surfaces by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). It was found that the Ni nanoparticles in-situ prepared in the RO with a mass fraction of 0.3% can reduce the wear scar diameter (WSD) of the steel ball by 36%. This is because, on the one hand, the Ni nanoparticles are adsorbed on the rubbed steel surfaces to repair or fill up the micro-pits and grooves thereon. On the other hand, Ni nanoparticles participate in tribochemical reactions with atmospheric O and steel substrate to form the tribochemical reaction film on the rubbed steel surfaces with the assistance of friction-induced heat and applied normal load. In addition, an amorphous carbon film is formed on the rubbed surface via the carbonization of base oil under the catalysis of Ni nanoparticles. The adsorbed Ni layer, the tribochemical reaction film, and the carbon layer comprise a composite tribofilm composed of amorphous carbon, polar fatty acid, metallic nickel, iron oxides, and nickel oxides on the rubbed steel surfaces, which contributes to significantly improving the antiwear ability and load-carrying capacity of the RO for the steel–steel sliding pair.
研究领域:
材料科学
·
摩擦学
·
纳米技术
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DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Yang Wang,Sidi Wang,Zhen Li,Liwei Sun,Xiaoyu Yang,Shaokun Tang
UiO-66 nanocrystals were synthesized by supercritical reaction crystallization with anhydrous zirconium chloride as the metal precursor, terephthalic acid as the ligand, and a small amount of N,N-dimethylformamide (DMF) and formic acid as cosolvents in supercritical CO2. In our work, the product UiO-66 obtained exhibits typical octahedral morphology with a specific surface area of 1092 m2/g and concentrated micropore diameters of 0.77, 1.05, and 1.73 nm. The thermal decomposition temperature of the product is 450 °C. It was found that the adsorption selectivity of UiO-66 for Congo red is much higher than those for methylene blue and methyl orange, which can reach up to over 1300 mg/g. The adsorption process conforms to the second-order adsorption kinetics curve, and the adsorption isotherm fits into the Langmuir model. Meanwhile, the adsorbent UiO-66 possesses good cycle stability and its adsorption performance remains excellent after seven cycles.
研究领域:
材料科学
·
环境科学
·
化学工程
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DOI
高影响因子
PLoS One
·
2024
·Vol. 19
·期 9
·e0309237
·
IF 2.9
Ruchika Kishor Jain,Anshumali,Prasad Sherekar,Amit Nayak,Shraddha Jaiswal,Komal Pimpalghare,Rajani Tumane,Aruna Jawade,Shubhangi Kailas Pingle,Sanvidhan G. Suke,Rajpal Singh Kashyap,Bibhuti Bhusan Mandal
Introduction
Information on bioavailable Iron (BAI) content in respirable coal dust (RCD) is crucial to address occupational health and safety, especially in preventing coal workers’ pneumoconiosis (CWP).
Materials and methods
In the present study, we determined BAI concentrations in seventy-seven coal samples collected from ten coal mining regions of Central India. The cytotoxic potential of BAI-RCD was established invitro by using alveolar epithelial (A549) and macrophage (U937) cell lines. The oxidative/antioxidant status, inflammations, and genotoxicity attributed to BAI-RCD exposure were evaluated and correlated with CWP pathophysiology.
Results
The mean BAI concentrations in the coal samples (n = 77) range from (275 to 9065 mg kg -1 ) and showed wide variability. Both cell lines were exposed to low (275 mg kg -1 ), moderate (4650 mg kg -1 ), and high (9065 mg kg -1 ) BAI-RCD samples showed significant ( p < 0.001) cytotoxicity in a dose-dependent manner (low < moderate < high) compared to the control. After BAI-RCD treatment, both cell lines showed a decrease in antioxidant stress measures (SOD, CAT, and GSH) and a significant ( p < 0.001) increase in oxidative stress parameters (NADPH, MPO, LPO, and PC). Furthermore, these cell line models demonstrated a statistically significant ( p < 0.001) dose-dependent increase in cytokines (TGF-β1, IL-1β, TNF-α, MCP-1, and IL-6 cytokines) and oxidative DNA damage marker (8-OH-dG).
Conclusion
Results indicated that the central India coals (even at low BAI content) may be accountable for inflammatory responses and cytotoxicity. Hence, BAI can be important characteristic to establish safety standards for coal dust exposure before active mining.
研究领域:
职业健康与安全
·
毒理学
·
环境科学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF GEOCHEMICAL EXPLORATION
·
2019
·
IF 3.472
Yunmei Cai,Jiandong Wu,Yanlin Zhang,Zheng Lin,Yisheng Peng
In order to clarify the distribution, sources, and ecological risk of 16 polycyclic aromatic hydrocarbons (PAHs) in sediments of mangrove wetland located in Shantou, twenty eight surface sediments were collected. The concentration of PAHs ranged from 79.74 to 853.17 ng g −1 dry weight. The concentration of PAHs of sediment in the high tides was higher than that in the low tides and higher in mangrove-planting area than that in non-mangrove growth zone. The spatial distribution of PAHs in the sediment of mangrove wetland was mainly influenced by input sources and tidal currents . The result of the principal component analysis-multiple linear regression model indicated that the major source of PAHs in the studied area was coal burning. However, the adverse effect of PAHs was weak to biota. Undeniably, a lot more systematic explorations are demanded in future to investigate the PAHs contamination in the mangrove wetlands in small–medium cities with the development of coastal zone economy.
研究领域:
环境科学
·
海洋生态学
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DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2021
·
IF 4.311
Dongxu Zhang,Qiyu Huang,Rongbin Li,Wei Wang,Xiangrui Zhu,Huiyuan Li,Yijie Wang
In this study, the effects of waxes on carbon dioxide hydrate formation and dissociation in asphaltene-containing emulsions were systematically investigated using a high-pressure stirred autoclave equipped with an on-line viscometer. The results showed that the presence of waxes strengthened the inhibiting effect of asphaltenes on hydrate formation. Specifically, the induction time increased and the amount of formed hydrates decreased with increasing wax content in water-in-oil emulsion containing asphaltenes. The results indicated that waxes increased the viscosity of emulsions. It was observed that wax crystals dispersed in the bulk phase and got adsorbed at water–oil interface through the synergistic effect with asphaltenes. Furthermore, waxes inhibited the dissociation of hydrates in these emulsions. Both the gas emission rate and the average rate of hydrate dissociation decreased with an increase in wax content. The possible mechanisms were proposed to illustrate the effects of waxes on hydrate formation and dissociation in asphaltene-containing water-in-oil emulsions.
研究领域:
化学工程
·
石油工程
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DOI
高影响因子
Forests
·
2022
·Vol. 13
·期 2
·253
·
IF 3.282
Xuan Li,Huijuan Bo,Jialei Zhu,Jiwei Zhang,Jun Hu,Fuyong Mu,Lishui Nie
This study aimed to explore the effects of different additives on tending shreds ofPlatycladus orientalis(L.) Franco. Two different additives (priming 0.2% and common compost 0.2%) combined with C, N, and P adjustment of raw material treatments were tested on the temperature, moisture, EC, pH, lignocellulose degradation rate, nutrient content, and toxicity of compost. Priming made the compost temperature rise rapidly, and the peak temperature of the composting group with priming reached 51 °C. At the end of composting, the moisture in each treatment from high to low was in the order: common compost > priming > C/N, C/P adjustment only > control group. The increase of EC in the treatments with additives was great, and the peak value of EC in the treatment of priming was 1.30 ms·cm−1, which was 3.9 times higher than that of the control group. At the end of composting, the decomposition rate of cellulose in priming compost was 1.7 times higher than that in the control group, and the hemicellulose decomposition rate in the common compost group was 3.2 times higher than that in the control group. By the end of composting, the pH value of the composts in additive treatments was above 7.0, and the pH value of the priming treatment was the highest (7.5). The highest content of organic matter was found in the priming treatment, which was 52%, 1.7 times higher than that in the control group. The total nutrient content (TN + K2O + P2O5) of additive treatments was higher than 5.0%, and the priming treatment was 2.7% higher than that of the control group. By the end of composting, the germination rate and germination index ranged from 88% to 91% and 60% to 81%. Except for the control group, the C/N ratio of other treatments decreased to below 25. Additives can accelerate the decomposition of raw materials, shorten the composting cycle, and improve the quality of composts, and the effect of adding priming is the most significant.Keywords:Platycladus orientalis(L.) Franco;compost;forest stand;Huairou District;nutrient cycling
研究领域:
林业学
·
生态学
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DOI
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Huang Ming-Qing,Zhang Ming,Zhan Shu-Lin,Chen Lin,Xue Zhen-Lin
In situ fragmentation bioleaching is a promising way to perform deep mining safely, economically, and in an environmentally friendly manner, where oxygen plays a critical role in microbial growth and mineral dissolution. However, the lack of oxygen limits the implementation of in-situ fragmentation bioleaching. To overcome this limitation, aeration was proposed, with saturated dissolved oxygen concentration as an important indicator. Orthogonal experiments were conducted to measure saturated dissolved oxygen concentration at various temperature, pH, and electrolyte (ferrous sulfate, ferric sulfate, copper sulfate, and sulfuric acid) concentration conditions. Experimental data were analyzed by Python programming language and least squares method to obtain a saturated dissolved oxygen concentration model. Results showed that temperature had the most significant effect on oxygen solubility, which was concluded by comparing the results of surface fitting based on the least squares method. At 30–40°C, the saturated dissolved oxygen concentration decreased faster as metal ions concentration increased. The conjoint effect of the five variables on oxygen solubility showed that pH was linearly negatively related to oxygen solubility. Additionally, a mathematical model was also proposed to predict the saturated dissolved oxygen concentration in in situ fragmentation bioleaching of copper sulfide ores. This work enables bioleaching processes to be modeled and controlled more effectively.
研究领域:
微生物学
·
采矿工程
·
环境科学
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DOI
PubMed
高影响因子
FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES
·
2025
·
IF 1.8
Lazang Gengga,Zifeng Chu,Bo Meng,Haichao Li
The precursors were formed in a concentrated salt system using the mutual synergy between TTAB (cetyltrimethylammonium bromide) and sodium sulphate at 100 °C, 300 rmp. Carbon nanorods were prepared by in-situ carbonisation of precursors at elevated temperatures. The prepared carbon nanorods were characterised by SEM, Raman, XRD, FT-IR and XPS. SEM results showed that the prepared carbon nanorods had a classical rod-like structure and most of them had lengths between 500 nm and 1.2 μm and diameters around 50 nm. Raman results showed ID/IG=0.839, a low degree of graphitisation and some defects in the structure of carbon nanorods as characterised by XRD. FT-IR and XPS analyses showed a variety of functional groups on the surface of carbon nanorods. Although the structure of the prepared carbon nanorods was not very perfect, it couldn’t be ignored that the method of preparing carbon nanorods using the interaction between Na2SO4 and TTAB was feasible.
研究领域:
材料科学
·
纳米技术
·
物理化学
·
功能材料
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产品关联
DOI
高影响因子
VETERINARY MICROBIOLOGY
·
2025
·
IF 2.7
Lisen Lin,Jiaqi Li,Chao Shang,Dapeng Li,Mingzhe Sun,Gaojie Song,Guangbo Qu,Xiao Li,Ran Zhu,Cuiling Zhang,Qinghao He,Guibin Jiang
Infectious bronchitis (IB) is an acute respiratory disease caused by the infectious bronchitis virus (IBV), spreads rapidly and manifests with diverse clinical signs, posing a major significant to the poultry industry. Metal ions such as copper are known to possess notable antiviral properties. Therefore, we evaluated the potential efficacy of copper-based pharmaceuticals against IBV. Results demonstrated that the copper-based drugs significantly protected DF-1 cells from IBV-M41 infection, markedly increasing cell activity and reducing IBV N-gene level. Furthermore, these drugs enhanced antiviral immunity and exhibited anti-apoptotic activity in vitro . In addition, these drugs increased the survival rates of infected chicken embryos and chicks while reducing developmental disruptions, thereby demonstrating robust antiviral effects. Pathological examinations revealed that lesions in the lungs, trachea, kidneys, spleen and other organs of treated groups improved to varying degrees, with a down-regulation of IBV N-gene level in chicks. Our study showed that the copper-based drugs exhibited anti-IBV-M41 both in vitro and in vivo , which provides a theoretical and experimental basis for exploring the anti-coronaviral effects of metal-based drug candidates.
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2019
·
IF 4.213
Jian Liu,Qilong Wang,Michael Adu-Frimpong,Qiuyu Wei,Yujiao Xie,Kangyi Zhang,Chunmei Wei,Wen Weng,Hao Ji,Elmurat Toreniyazov,Ximing Xu,Jiangnan Yu
Isoliquiritigenin (ISL) has a great variety of pharmacological effects especially liver cancer therapy, but its poor solubility, bioavailability and liver targeting have limited its clinical use. In order to solve the aforementioned shortcomings, the TPGS-modified proliposomes loaded with ISL (ISL-TPGS-PLP) was prepared in this study. ISL-TPGS-PLP was fabricated via thin-film dispersion method and was characterized by the appearance, particle size, zeta potential and morphology. HPLC was used to evaluate entrapment efficiency (EE), in vitro release and stability of ISL-TPGS-PLP single or combined while appropriate physicochemical parameters were measured with DLS . Meanwhile, the pharmacokinetics and tissue distribution were also studied after oral administration. The results demonstrated that ISL-TPGS-PLP had a mean size of 23.8 ± 0.9 nm , high EE of 97.33 ± 0.40%. More importantly, nearly 90% ISL was released from ISL-TPGS-PLP within 24 h while only 50% was released from ISL suspension. In the pharmacokinetics study, the area under the curve (AUC 0-24h) of ISL-TPGS-PLP was 1.53 times higher than that of ISL suspension. The Tissue distribution study showed that the ISL released from ISL-TPGS-PLP was higher in the liver than the free ISL suspension. Altogether, ISL-TPGS-PLP could ameliorate the ISL solubility, bioavailability and liver targeting ability, suggesting that ISL-TPGS-PLP could serve as a promising nanocarrier for liver cancer therapy.
研究领域:
药剂学
·
肿瘤学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）