SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Lixian Huang,Jinzhe Liang,Jiaqi Lu,Min He,Yidong Bin,Yanni Luo,Shulin Zhao,Shengqiang Hu,Liangliang Zhang
The integration of ratiometric fluorescence imaging in the second near-infrared (NIR-II) window with chemodynamic therapy (CDT) and photodynamic therapy (PDT) represents a promising strategy for the accurate diagnosis and efficient treatment of deep tumors. In this study, we developed a smart nanoprobe, D/UCNP-MB@SiO 2 - t AuCuNCs (D/UCMSA), with simultaneously triggering reactive oxygen species (ROS) and NIR-II fluorescence for enhanced chemodynamic/ photodynamic therapy and ratiometric imaging-guided tumor resection. Upon 980 nm laser irradiation, D/UCMSA emit up-converted light at 660 nm, which activates the loaded methylene blue (MB) to produce singlet oxygen ( 1 O 2 ) for PDT. Simultaneously, the down-converted emission at 1525 nm serves as a reference signal for ratiometric imaging. The D/UCMSA surface is decorated with copper-doped gold nanoclusters ( t AuCuNCs) stabilized by triphenylphosphine-3,3′,3′′-trisulfonic acid, which undergo ligand exchange with overexpressed glutathione in the tumor microenvironment. Upon 808 nm laser excitation, this ligand exchange process activates fluorescence emission at 1085 nm. The incorporation of copper endows t AuCuNCs with peroxidase- and catalase-like activities, enabling the catalytic conversion of hydrogen peroxide (H 2 O 2 ) into hydroxyl radicals (·OH) and oxygen (O 2 ) within the tumor microenvironment. This dual enzymatic activity amplifies the CDT efficacy in tumor treatment and reshapes the tumor microenvironment, providing sufficient O 2 for 1 O 2 generation during PDT and enhancing the efficiency of combined CDT/PDT. Following with orthotopic injection of the D/UCMSA, irradiation with 808 nm and 980 nm lasers successfully enabled precise tumor resection guided via ratiometric NIR-II fluorescence imaging, combined with effective CDT/PDT. This study demonstrates a novel approach to developing integrated nanoplatforms for accurate tumor diagnosis and efficient treatment.
研究领域:
生物医学工程
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纳米医学
·
肿瘤学
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DOI
高影响因子
Small
·
2025
·
IF 12.1
Lixian Huang,Fei Lv,Yidong Bin,Jingjin Zhao,Caiying Li,Shulin Zhao,Shengqiang Hu,Liangliang Zhang
Hydrogen sulfide (H 2 S) is a vital gaseous signaling molecule that plays a central role in various physiological and pathological processes. Given the complementary advantages of fluorescence (FL) and photoacoustic (PA) imaging, there is a growing demand for dual-ratiometric probes that enable precise in vivo monitoring of H 2 S levels. In this study, the use of 2-mercapto-1,3,4-thiadiazole (MTD) as a novel recognition group of H 2 S is presented for the first time, following conjugation with cyanine dyes to obtain a new PA probe Cy-MTD. To achieve dual-ratiometric imaging, Cy-MTD is incorporated into down-conversion nanoparticle (DCNP), resulting in the creation of a pioneering NIR-II FL/NIR-I PA dual-ratiometric nanoprobe DCNP@Cy-MTD. Cy-MTD undergoes the blueshift in absorption from 840 to 670 nm after reaction with H 2 S, enabling NIR-I ratiometric PA imaging of H 2 S by measuring the ratio of PA signal at 670 and 840 nm (PA 670 /PA 840 ). In addition, due to the strong absorption of Cy-MTD ≈840 nm and the overlapping between the absorption spectrum of Cy-MTD and 808 nm excitation band of DCNP, the 808 nm-excited FL emission (F 1550 nm,808Ex ) of DCNP in DCNP@Cy-MTD nanoprobe is quenched through the competitive absorption, while it is restored upon the interaction with H 2 S because of the blueshift in absorption of Cy-MTD. Using the stable FL emission of DCNP under 980 nm excitation (F 1550 nm,980Ex ) as the reference signal, NIR-II ratiometric FL imaging (F 1550 nm,808Ex /F 1550 nm,980Ex ) of H 2 S is achieved. The dual-ratiometric response features of the DCNP@Cy-MTD nanoprobe offer a significant advancement over traditional single-signal or single-modality imaging techniques. By providing enhanced accuracy and reliability, this probe allows for the diagnosis of hepatitis by monitoring the H 2 S, surpassing the capabilities of conventional histopathological methods, which provides a new way for more effective diagnostic strategies for liver diseases.
研究领域:
生物医学工程
·
分子影像学
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肝脏疾病研究
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Sheng Zhang,Zhenyang Liu,Linlin Mao,Jian Wu,Di Zhang,Ruiyuan Liu,Li Qi
High quality visualization of live mammalian embryo is an important requirement for studying fetal development. Photoacoustic tomography (PAT) and optical coherence tomography (OCT) are two advanced imaging modalities that have been utilized for embryonic imaging separately. However, high contrast, multiscale, and deep tissue imaging of embryos in vivo remains challenging. Here, we demonstrate multimodal contrast-enhanced imaging of live embryos by gold nanostar-meditated near-infrared I (NIR-I) PAT and near-infrared II (NIR-II) OCT. We investigate intravenous and intravaginal administration of gold nanostars, and significant enhancement of signal, image contrast, and imaging depth are achieved for both PAT and OCT. Our findings may facilitate detailed studies of the morphological and functional changes during embryonic development.
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高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Xingchen Li,Ming Hao,Annan Liu,Lei Li,Maja D. Nešić,Bai Yang,Weiwei Liu,Quan Lin
Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the head and neck region, and its treatment is limited by hypoxia and inadequate oxygen supply. Continuous oxygen delivery combined with photodynamic therapy (PDT) is the key to addressing this issue. Here, a dual-enzyme activity sea urchin-like Au@Pt-Ce6-HN-1 nanoplatform was designed to serve as an "oxygen pump" to alleviate tumor hypoxia for synergistic photodynamic/photothermal therapy (PTT). In this design, the photosensitizer chlorin e6 (Ce6) is covalently linked to the Au@Pt nanozyme for PDT treatment. The Au@Pt nanozyme exhibits catalase-like activity, continuously decomposing H 2 O 2 in the tumor microenvironment to enhance O 2 levels, thereby achieving efficient PDT. Furthermore, Au@Pt can perform PTT and increase oxygen levels under NIR-II light to further promote PDT. The Au@Pt nanozyme also exhibits peroxidase-like activity, generating ·OH for chemodynamic therapy (CDT). Additionally, HN-1 guides the direction of "sniping" OSCC, and its high specificity benefits Au@Pt-Ce6-HN-1 at the tumor site. Au@Pt-Ce6-HN-1 exhibits bright fluorescence (FL), strong CT signal, and photothermal imaging capabilities, laying the foundation for subsequent guided PDT/PTT. This nanoplatform, which combines advantages such as continuous oxygen production, tumor targeting, and multimodal imaging, is expected to provide valuable insights into the treatment of OSCC. Statement of Significance Accurate clinical diagnosis and treatment of OSCC are challenging. We report a dual-enzyme activity sea urchin-like Au@Pt-Ce6-HN-1 nanoplatform, serving as an "oxygen pump" to guide photodynamic therapy (PDT) and photothermal therapy (PTT) for OSCC. This nanoplatform targets OSCC for preoperative CT diagnosis and offers fluorescence visualization for surgical navigation, demonstrating potential in clinical cancer detection and surgery guidance. This innovative approach addresses OSCC hypoxia and enhances treatment efficacy through continuous oxygen production, tumor targeting, and multimodal imaging, significantly improving patient outcomes in OSCC treatment.
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PubMed
高影响因子
JOURNAL OF RARE EARTHS
·
2024
·
IF 5.2
Jiantong Wang,Changshuai Gong,Sihan Yang,Qi Zhu,Xuejiao Wang,Ji-Guang Li
Phosphor-converted near-infrared light-emitting diodes (NIR pc-LEDs) are finding applications in various fields including food quality analysis, biomedical imaging, night vision, and biomedicine. The crucial factor in the development of NIR pc-LEDs devices lies in the advancement of high-performance broadband NIR phosphors. In this work, novel Cr 3+ -activated silicate phosphors NaRESi 3 O 9 (RE = Y, Lu, Sc) are reported. This silicate has a special 3D network structure in which RE has four different sites, forming four octahedrons and providing suitable occupation sites for Cr 3+ . The phosphors demonstrate a wide emission spectrum ranging from 750 to 1450 nm when excited by light at 468 nm. The full width at half maximum (FWHM), which benefits from the presence of Cr 3+ ions occupying multiple sites, is measured to be 203 nm. Notably, the strongest emission peak is observed at a longer wavelength of 984 nm compared to most other systems activated by Cr 3+ . The Na 3 ScSi 3 O 9 lattice provides a weak crystal field ( Dq / B = 1.97) and weak phonon-photon coupling for Cr 3+ , and the integrated emission intensity of Na 3 ScSi 3 O 9 :0.03Cr 3+ is 4.66 times stronger than that of Na 3 YSi 3 O 9 :0.03Cr 3+ .
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POLYMER
·
2026
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IF 5.1
Junqi Zhao,Zicheng Chen,Jiahao Huang,Jingxian Zhang,Langhuan Huang,Shaozao Tan,Yongqiang Xiong
Bacterial infections and excessive oxidative stress severely impede the wound healing process, and the development of multifunctional wound dressings with antibacterial, antioxidant, and biosafety properties is of great clinical significance. In this study, DAHA/CMCS/Ag@PDA (DCA) injectable hydrogels were successfully prepared by dual-dynamic crosslinking of Schiff base and hydrogen bonds. Dopamine graft-modified oxidized hyaluronic acid (DAHA) and carboxymethyl chitosan (CMCS) constructed a dynamic and reversible cross-linking network of the hydrogel, which endowed the hydrogel with excellent injectable, self-healing, shape-adaptive, and high adhesion properties. Meanwhile, DAHA and Ag@PDA, which are rich in catechol groups, provided the hydrogels with highly efficient synergistic antioxidant properties, which could effectively scavenge various free radicals. In addition, the loading of Ag@PDA endowed the hydrogel with excellent Ag + release and NIR-II photothermal conversion properties, and DCA2 could realize efficient killing (99.6%) of S. aureus and E. coli through the synergistic action of Ag + slow release and NIR-II photothermal, which could effectively promote the healing of bacterial-infected wounds. In conclusion, this study provides a new design strategy for hydrogel dressings for the treatment of bacterial-infected wound healing through the multifunctional integration of NIR-II photothermal synergistic antibacterial and antioxidant properties.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yan Du,Chunlei Shan,Yuchan You,Minjiang Chen,Luwen Zhu,Gaofeng Shu,Gang Han,Liming Wu,Jiansong Ji,Hong Yu,Yongzhong Du
Lenvatinib (LEN) combined with immune checkpoint PD-1 blockade is among the most effective treatment strategies for advanced hepatocellular carcinoma (HCC) and has been deemed a breakthrough therapy by the Food and Drug Administration. However, this combined strategy is associated with disadvantages such as non-tumor targeted aggregation and low oral bioavailability after long-term administration of LEN, and some patients show poor responses to immunotherapy as well. To overcome these drawbacks, SP94 peptide-modified thermal-sensitive micelles (SP94-poly(ethylene glycol)-poly(acrylamide- co -acrylonitrile, SP94-PEG-p(AAm- co -AN)) with an upper critical solution temperature (UCST) of 43 °C were designed. The micelles were further co-loaded with LEN and IR-1061-Acridine (IR-1061-AcD) to simultaneously achieve small molecule targeted drug, photothermal and photodynamic therapy for HCC under the guidance of near-infrared second-region (NIR-II) fluorescence imaging. After intravenous injection, the co-loaded micelles (SP94-PEG-p(AAm- co -AN)/LEN/IR-1061-AcD, SPLI) showed greater accumulation at tumor sites because SP94 has a high affinity for glucoregulatory protein 78 (GRP78), which is overexpressed on HCC cells. Given these HCC-targeted effects, SPLI appeared useful for NIR-II fluorescence imaging, enabling real-time tumor monitoring and surgical resection. In addition, SPLI could extensively induce immunogenic cell death in tumors, resulting in beneficial systemic immune responses. When further combined with anti-PD-1 immunotherapy, SPLI displayed a superior potential in suppressing tumor growth and metastasis while avoiding immune inhibition. Taken together, our study suggests that the aforementioned combinational strategy could serve as a desired multifunctional approach for HCC management.
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Applied Materials Today
·
2023
·
IF 8.3
Wei Meng,Lian-Ying Zhang,Xiao-Tong Chen,Feng-Jun Ge,Peng Huang,Yong-Jian Chen,Ting Huang,Nai-Han Huang,Yi-Ming Zou,Wen-Hua Zhang,Qun Zhang,Jin-Xiang Chen
The drug-resistant bacterial infection has seriously threatened human health and thus it is urgent to develop new and effective drugs to overcome the antibiotic resistance crisis. Herein, a near-infrared-II (NIR-II) region light-responsive Pd rough nanoshells on metal-organic framework (MOF) of UiO-66-NH 2 (UiO-66-NH 2 @Pd shell ) has been developed for the first time, which was further loaded with the photosensitizer IR780 to ultimately yield UiO-66-NH 2 @Pd shell -IR780 (UPI) as the core-shell theranostic nanoplatform. The Pd rough nanoshells demonstrate promising photothermal conversion efficiency, superoxide dismutase (SOD)-like activity, and light-enhanced catalase (CAT)-like activity. In the non-healing wound inflammatory microenvironment with overexpression of reactive oxygen species (ROS) caused by subcutaneous bacterial infection, Pd rough nanoshells in UPI transforms ROS, such as O 2 •− , into H 2 O 2 and efficiently catalyzes the decomposition of the formed and endogenous H 2 O 2 to produce O 2 . The O 2 production process could be enhanced by NIR-II light to facilitate photodynamic efficacy of IR780 to prime rapid clearance of methicillin-resistant Staphylococcus aureus (MRSA) through biofilm inhibition, along with down-regulation of hypoxia inducible factor-1α (HIF-1α) and relief of inflammation, thus promote wound healing. Moreover, UPI exhibits negligible biotoxicity in vitro and in vivo . This work provides a method for preparing promising Pd-based nanomaterials toward subcutaneous infection treatment and wound healing.
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高影响因子
Inorganic Chemistry Frontiers
·
2024
·Vol. 11
·期 20
·7098-7109
·
IF 6.1
Fengmei Zhu,Yuan Gao,Jianbei Qiu
Presently, there is a need to expand the near-infrared (NIR) emission range, especially in the bio-transparent window, to improve the internal quantum efficiency and external quantum efficiency, and to minimize the shortcomings of luminescence properties. In this work, an ultra-broadband NIR emission Cr3+-doped Cs2NaLuCl6 all-inorganic metal halide perovskite phosphor was synthesized by a grinding–sintering method. Under 300 nm excitation, Cs2NaLuCl6:Cr3+ phosphors exhibited ultra-broadband NIR emission of 800–1400 nm, which covers nearly the entire NIR-I (700–1000 nm) and NIR-II (1000–1400 nm) regions; the emission peaked at around 960 nm, with a full width at half maximum of ∼182 nm. Noticeably, the internal- and external-quantum efficiencies of the optimized Cs2NaLuCl6:Cr3+ phosphor were as high as ∼92.9% and ∼60.75%, respectively. We showed through analysis and simulation that the weak crystal field strength is conducive to increasing the odd term of the crystal field, and therefore it partially released the restriction imposed by the parity prohibition law and improved the energy absorption efficiency of Cr3+. As the size of the phosphor approaches the wavelength of NIR luminescence, the apparent Mie scattering effect starts to take effect, which enhances the light extraction of NIR luminescence in the radial direction.
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高影响因子
Small
·
2025
·
IF 12.1
Xiaozhao Wang,Jing Li,Zhengtao Luo,Zhimin Gao,Yongxin Huang,Jiamin Luo,Xinyi Wang,Yaru Zhang,Meiling Tan,Zhiyao Hou
Insufficient adenosine triphosphate (ATP) can reduce the synthesis of heat-stress-induced heat shock proteins (HSPs) to promote the efficiency of mild photothermal therapy (mPTT), thus the rational design of ATP-exhausted strategy based on nanotechnology is an effective approach to resuscitate mPTT. Herein, Nd 3+ doped nanocrystals (NaYF 4 :Nd@CaF 2 , Nd-NCs) modified hollow mesoporous manganese oxide (H-MnO x ) nanocomposite (H-MnO x @Nd-NCs, MN) is synthesized, and loaded with glucose transporters (GLUTs) inhibitor KL-11743, noted as MN-KL nanozyme. In tumor microenvironment (TME), MN-KL can react with overexpressed glutathione (GSH) to release KL-11743, which can suppress the synthesis of intracellular ATP at the source by blocking glucose uptake to inhibit HSPs expression, meanwhile, MN-KL catalyzes the production of ·O 2 − / 1 O 2 /·OH and lipid peroxidation (LPO) to cleave existing HSPs. Through a two-pronged strategy with ATP inhibition and oxide accumulation, reducing the level of HSPs can be guaranteed for achieving efficient mPTT in both subcutaneous and in situ tumor models in mice. During this process, Nd-NCs can absorb near-infrared light and convert it into heat, and the quenched fluorescence of Nd-NCs by H-MnO x can be recovered through GSH-triggered biodegradation in tumors, thus the modification of Nd-NCs not only provides photothermal effect but also enables MN to own TME-activated fluorescence imaging.
研究领域:
生物医学工程
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肿瘤学
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纳米医学
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DOI
PubMed
高影响因子
ACS Sensors
·
2022
·
IF 9.618
Zhidong Qiu,Chaobang Zhang,Liangliang Zhang,Shulong Wang,Shengqiang Hu,Shulin Zhao
The interaction between light and biological tissues in the second near-infrared (NIR-II) window is weak, which can effectively reduce the scattering and absorption of incoming light by biological tissues and enhance the resolution and sensing ability of in vivo photoacoustic (PA) imaging. In particular, tissues that carry blood and water produce the lowest PA background in the wavelength range of 1050 to 1150 nm. However, the development of the NIR-II PA probe for the above window faces great challenges. To tackle this challenge, the reduction–reoxidation of an organic dye was used to develop a PA imaging probe (Hydro-1048) as the first NIR-II PA probe of a hydroxy radical (·OH) for molecular imaging in deep tissue. The ·OH oxidized the C–N single bond in Hydro-1048 to double bonds, which formed Et-1065. This conversion extended the conjugate system of the molecule and shifted the absorption peak from 520 to 1065 nm, which resulted in a strong PA signal after irradiation with a 1065 nm laser. At a detection limit of 0.6 nM, a good linear relationship within the range of 5–1000 nM was obtained for the PA signal intensity versus the concentration of ·OH. The developed NIR-II PA probe can be used for the noninvasive high-resolution imaging of ·OH in deep tissue, and the PA imaging of ·OH can also be used to visually monitor in situ pathological processes related to hepatitis.
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MICROCHEMICAL JOURNAL
·
2024
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IF 4.9
Wenfang Huang,Shulong Wang,Min Wu,Xinping Huang,Qifei Mo,Jiayao Xu,Rong Wang,Qingmin Wei
Hydrogen peroxide (H 2 O 2 ) plays a crucial role as a signaling molecule within cells. The concentration of H 2 O 2 differs in inflammation, cancer, cardiovascular and cerebrovascular diseases and neurodegenerative diseases compared to normal tissue. Therefore, the accurate determination of H 2 O 2 is important for the diagnosis and understanding of pathogenesis of disease. However, to accurately determine H 2 O 2 levels in vivo, highly sensitive biosensors and deep tissue penetrating imaging techniques are required. To address this challenge, we developed a biocatalytic porous structure composed of metal–organic frameworks (MOFs) that encapsulates horseradish peroxidase (HRP) and 3, 3′, 5, 5′-Tetramethylbenzidine (TMB) in situ, enabling biomineralization while maintaining the catalytic activity and stability of natural enzymes. This leads to the formation of a hypersensitive biological probe BSA@HRP@TMB@ZIF-8 (BHTZ). BHTZ is an activatable near-infrared probe and its absorption signal in the second near-infrared window (1030 nm) is activated in the presence of H 2 O 2 . The findings indicate that the BHTZ probe has high sensitivity, low toxicity and high stability, and can be utilized to detect H 2 O 2 levels in mice with drug-induced liver injury, such as that caused by lipopolysaccharide (LPS) in vivo. These results not only provide a highly sensitive and deep imaging method for monitoring physiological changes in H 2 O 2 levels in vivo, but also broaden the potential applications of MOFs in biomedical research and diagnostics.
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