Advanced Science
·
2025
·
IF 14.1
Dongjian Song,Qiuliang Liu,Zechen Yan,Qi Wang,Meng Su,Hui Zhang,Longyan Shi,Yingzhong Fan,Qian Zhang,Heying Yang,Da Zhang
This study investigates the molecular mechanisms by which superparamagnetic iron oxide nanoparticles (SPIONs) loaded with the WSGC peptide (WSGC@FA@PEG/PEI-SPIONs)—a 40-amino acid polypeptide derived from apoC-III—modulate chemotherapy resistance in gastric adenocarcinoma (GA). Emphasis is placed on their role in regulating mitophagy and mitochondrial homeostasis via the Notch signaling pathway. The physicochemical properties of WSGC@FA@PEG/PEI-SPIONs are thoroughly characterized, demonstrating favorable biocompatibility, stable size distribution, and efficient peptide loading. In vitro experiments show that these nanoparticles significantly inhibit GA cell proliferation, migration, and invasion by downregulating mitophagy-associated proteins (LC3, PINK1, and Parkin), primarily through modulation of the Notch pathway. In vivo studies, using a GA nude mouse model, confirm the therapeutic potential of WSGC@FA@PEG/PEI-SPIONs, revealing marked tumor growth inhibition and increased apoptotic activity. Collectively, the findings highlight the WSGC peptide as a promising therapeutic agent for overcoming chemotherapy resistance in GA by targeting the Notch signaling pathway and suppressing mitophagy, thereby presenting a novel strategy for polypeptide-based cancer therapy.
研究领域:
纳米医学
·
肿瘤学
·
分子生物学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 36
·29300-29310
·
IF 4.6
Xiao Zhou,Huali Huang,Lifeng Luo,Chenxi Cui,Hongmian Jiang
Pancreatic cancer, a highly aggressive solid tumor, presents multiple challenges in treatment. Although surgical resection is the primary treatment for early-stage non-metastatic cases, the deep abdominal location of the pancreas complicates precise intraoperative localization, adversely affecting surgical outcomes and patient prognosis. To address these clinical challenges, we aim to develop an innovative intraoperative navigation platform for precision tumor-targeted delivery. We systematically evaluated the tumor-targeting specificity and tissue-penetrating capacity of three hydrosoluble polymers, i.e., dextran, PEG, and HA, in pancreatic carcinoma models. The uptake kinetics of these materials in cancer cells and their penetration dynamics across complex tumor matrices were quantitatively evaluated via in vitro assays and three-dimensional tumor spheroid models. Complementary in vivo validation involved the use of PNAC-1 cell subcutaneous xenograft models to map the spatiotemporal distribution and tumoral accumulation of the lead candidates. Results demonstrated that dextran-FITC had a superior tumor-targeting performance, showing a significantly higher fluorescence intensity than PEG-FITC and HA-FITC. These preliminary data reveal significant heterogeneity in the intertumoral localization efficiency among the polymer carriers, establishing a mechanistic foundation for intraoperative navigation. Through rational optimization of carrier selection and delivery modalities, this platform aims to enhance surgical precision, minimize off-target effects, and ultimately improve clinical outcomes.
研究领域:
肿瘤学
·
外科研究
·
生物医学工程
·
分子成像
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Yi Zhang,Xinglin Huang,Yuqing Li,Haiyang Zhang,Yezhong Chen,Jinlin Lu,Jianxin Li,Yansheng Yin
In order to alleviate the depletion of petrochemical resources and the resulting environmental pollution problems, it is becoming more and more important to find bio-based polymer materials that can partially or completely replace petrochemical resources. In this study, an epoxidized soybean oil-based antifouling polyols (ESO-PEG) coupled with brush-like anti-fouling polyethylene glycol on the side chain was prepared by epoxy ring opening reaction between epoxy soybean oil and polyethylene glycol monomethyl ether. Secondly, a kind of gemini quaternary ammonium salt antibacterial monomer containing a hydroxyl group (OHGQAS) was synthesized. A series of bio-based waterborne polyurethane coatings with both antifouling and antibacterial activities were synthesized by introducing prepared ESO-PEG and OHGQAS. Antibacterial and antifouling chains suspended on polyurethane side or end chains with good mobility. The effects of ESO-PEG and OHGQAS monomer on the particle size of waterborne polyurethane emulsion, bulk and surface structure, antibacterial and anti-fouling properties of waterborne polyurethane coatings were discussed. The results showed that the introduction of ESO-PEG improved the antifouling performance of polyurethane coatings (antibacterial ratio 96.68 %). In addition, after the introduction of OHGQAS into the structure of waterborne polyurethane, the antibacterial effect of the coating was further improved, that the antibacterial ratio of polyurethane coating with OHGQAS was as high as 99.99 %. These epoxy soybean oil-based waterborne polyurethane coatings hold great promise for antibacterial and antifouling applications.
研究领域:
材料科学
·
高分子科学
·
环境科学
·
表面工程
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2025
·Vol. 18
·期 17
·4165
·
IF 3.2
Heng Zhang,Yiping Yang,Shengchao Yang,Yuchang Qin,Xuan Lv,Lin Cui,Wei Jia,Zhiyong Liu
The impact of encapsulating gemcitabine (GEM) into nanoparticles on its delivery remains underexplored, with the potential benefits of targeted drug delivery and stimuli-responsive release yet to be fully clarified. Herein, we designed a near-infrared (NIR) light-responsive polymeric nanoparticle, ZnPc@P(PEG-CMA-TKGEM), which integrates reactive oxygen species (ROS) generation and cell imaging capabilities. The self-assembled polymeric micelles exhibit a hydrodynamic size of ~134 nm. Under NIR irradiation, the cumulative drug release rate reaches 51% within 48 h, which is three times higher than that of the non-irradiated control group. In cytotoxicity assays, the cell viability of the NIR-irradiated drug-loaded group is approximately 17%, while that of the NIR-irradiated blank group (without drug loading) remains above 80%. These results confirm that the nanocarriers successfully deliver GEM to target cells and achieve controlled drug release via NIR stimulation.
研究领域:
纳米医学
·
癌症治疗
·
药物递送系统
·
高分子化学
·
光动力治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 39
·18426-18435
·
IF 6.97
Meng Duan,Fangfang Xia,Tianliang Li,Joseph G. Shapter,Sheng Yang,Yongying Li,Guo Gao,Daxiang Cui
This work explored the application of matrix metalloproteinase 2-targeted superparamagnetic nanoprobes for magnetic resonance imaging (MRI), near infrared (NIR) fluorescence imaging and photodynamic therapy of tumors. PEG, PAMAM (G5) and matrix metalloproteinase 2 (MMP2) were attached to the surface of carboxylated Fe3O4 nanoparticles (NPs) using a chemical coupling method and then finally loaded with the photosensitizer chlorin e6 (Ce6). In vitro and in vivo experiments demonstrated that the Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes exhibited excellent stability, precise tumor targeting and biocompatibility. Furthermore, the fluorescence properties of Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes were analogous to Ce6 and could be employed for fluorescence imaging. Meanwhile, the Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes have also been shown to be effective as contrast agents for T2-weighted MRI. The target molecule MMP2 enhanced the tumor targeting ability of Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes. Additionally, the Fe3O4-PEG-G5-MMP2@Ce6 nanoprobes significantly inhibited tumor growth compared with PBS and free Ce6. This work will inspire greater enthusiasm for the construction of multifunctional magnetic nanoplatforms for biomedical applications.
研究领域:
生物医学工程
·
材料科学
·
纳米技术
·
癌症研究
·
医学影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
·
2020
·
IF 2.169
Yutong Wang,Yanfang Ding,Changyuan Wang,Meng Gao,Youwei Xu,Xiaodong Ma,Xinyi Ma,Hongxia Cui,Lei Li
Fenretinide (4-HPR), a synthetic retinoid, has shown its antitumor activity in many tumor types with low cytotoxicity to normal cells and high clinical safety. However, the low water solubility limits its further biological applications. To increase solubility, 4-HPR was conjugated with methoxy polyethylene glycol carboxylic acid (mPEG2K-COOH) by an ester linkage between the phenol hydroxyl of 4-HPR and the carboxyl of mPEG2K-COOH. The 4-HPR-PEG2K conjugate micelles had mean size of 76.70 ± 1.248 nm with a narrow distribution and a low critical micelle concentration. In vitro cytotoxicity studies showed the micelles have higher cytotoxicity to A2780s and MCF-7 cells. Its IC50 was 4.7 and 4.1-fold lower than the free 4-HPR, respectively. Importantly, in vivo pharmacokinetic studies, the AUC of 4-HPR was found to be 2.3-fold higher in 4-HPR-PEG2K micelles compared to free 4-HPR. And the 4-HPR-PEG2K micelles had higher antitumor activity. Meanwhile, the histopathology analysis exhibited that the micellar treatment decreased the viability of A2780s cells and increased the level of induced apoptosis. Therefore, the enhanced activity of 4-HPR by the method of conjugation with mPEG2K-COOH could hopefully provide new insights into the matter of ovarian cancer and breast cancer treatment.
研究领域:
药学
·
肿瘤学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Electronic Materials
·
2026
·
IF 4.7
Xue-Ting Zhang,Meng-Nan Liu,Sai Wang,Gang Zheng,Ru Li,Jun Zhang,Xiuping He,Wen-Peng Han,Yun-Ze Long
To overcome the limitations of flexible sensors relying on external power supply and single-mode detection, this study proposes a self-powered flexible sensor based on PVDF-TrFE-co-PEG Diamine@ionic liquid nanofiber membrane. This sensor integrates capacitive and piezoelectric dual modes within a compact sandwich structure, enabling high-sensitivity static pressure detection and self-powered dynamic signal generation without requiring an external power source or mode switching. This sensor exhibits ultrahigh sensitivity (32.28 kPa–1, pressure < 12.5 kPa) in capacitive mode and can generate an open-circuit voltage as high as 1.51 V in piezoelectric mode, achieving efficient mechanical energy-to-electrical energy conversion. This will demonstrate broad application prospects in the fields of passive wearable electronics and electronic skin, providing a methodology for designing multifunctional integrated sensing systems.
✓ 已验证
产品关联
DOI
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2017
·
IF 4.873
Changzhen Liu,Shibing Ye,Jiachun Feng
For poly (lactic acid) (PLA) nanocomposites , improving the dispersion of nanofillers and promoting the crystallization rates of PLA matrix are crucial to the final properties. Here, we proposed a facile “freezing-dried masterbatch” strategy to fulfill the two goals simultaneously in the preparation of PLA/graphene composites. After graphene oxide (GO) sheets are dispersed in poly (ethylene glycol) (PEG) aqueous solution and hydrazine hydrate is added to transform GO sheets into graphene sheets , the resulting mixture was freezing-dried to get the masterbatch of PEG and graphene. By simply melt blending the masterbatch with pristine PLA, PLA composites with excellent mechanical properties and thermal stability are prepared. For example, with 0.25 wt % of graphene, the composite prepared using the freezing-dried masterbatch has a tensile stress of 61.6 MPa, a tensile modulus of 1256 MPa, and a toughness value of 4.86 MJ/m 3 . The values are respectively 50.9, 125.5, 200.0% higher than that of the control sample of PLA-PEG and 11.5, 25.5, 124.0% higher than that of PLA composite prepared by directly blending with 5 wt % PEG and 0.25 wt % graphene. Morphological observations and crystallization behavior investigations reveal that graphene shows well dispersion in PLA composites, and the crystallinity and crystallization rates of PLA are greatly improved, which may be the reason for the improved mechanical properties .
研究领域:
材料科学
·
高分子化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zhengzheng Wang,Xiaoyan Feng,Fangbin Xiao,Xuekun Bai,Qian Xu,Hengyi Xu
Staphylococcus aureus ( S. aureus ) is one of the pathogens that seriously endanger human health and are widespread in nature. Food poisoning incidents caused by S. aureus frequently break out. Therefore, it is significant that a rapid and accurate method is established for S. aureus detection. In this study, a strategy using magnetic separation combine with fluorescence analysis was proposed for S. aureus detection. Boric acid can selectively enrich compounds with cis -diol groups through a reversible esterification reaction, and has been widely used in the identification of sugars and glycosylated molecules. As a boric acid derivative, 3-aminophenylboronic acid (APBA) has the advantages of stability, low cost, easy synthesis, etc. Therefore, using APBA as a recognition molecule to prepare magnetic nanomaterials can achieve the enrichment of S. aureus . In order to reduce steric hindrance and ensure the dominant orientation of APBA, thus improving the enrichment performance of target bacteria. The polyethylene glycol (PEG) was used as a mediator to prepare PEG-mediated APBA functionalized magnetic nanomaterials (APBA-PEG-MNs). In addition, this study used FITC-pig IgG to label S. aureus because the Fc fragment of IgG to bind protein A on the surface of S. aureus and realized the detection of S. aureus by detecting fluorescence. Under the optimal experimental conditions, the capture efficiency of APBA-PEG-MNs for S. aureus with the concentration of 2.7 × 10 6 CFU/mL in spinach, juice and pool water spiked samples was 92.6%, 91.6%, 91.6%. The limit of detection of the strategy in spiked samples was 2.7 × 10 2 CFU/mL within 80 min.
研究领域:
生物化学
·
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
POLYMER
·
2023
·
IF 4.6
Xiaoqiong Wang,Leisha Cui,Jing Hong,Zhaojun Wang,Jiawei Li,Zhongqing Liu,Zhanchi Zhu,Ying Hao,Guosheng Cheng,Junhong Jiang
Because of the shortage of donors, alveolar stem cells transplantation is under development as an important and promising treatment for end-stage lung disease. Currently, the acquisition of functional lung cells is mostly performed by two dimensional (2D) culture and suffers from low directed differentiation efficiency. It is particularly critical to replicate the three dimensional (3D) growth of lung cells, as cells grow in 3D microenvironment in vivo . In this work, we develop a 3D biomimetic composite hydrogel system based on the in situ crosslinking of the synthetic polymer 4 arm acrylated-poly(ethylene glycol) (4PEG-AC), the thiolated natural polymer hyaluronic acid (HA-SH), and the thiolated polypeptide (GSE4-SH) via a Michael-type “click” reaction under physiological conditions at pH 7.8. The PEG/HA/GSE4 hydrogel with a sheer modulus of 104.1 ± 9.9 Pa was used for 3D growth of bone marrow mesenchymal stem cells (BMSCs), displaying excellent biocompatibility. Covalent conjugation of GSE4 peptide in hydrogel promoted the differentiation of BMSCs into alveolar epithelial type 2 cells (AEC2s) with a AEC2s-differentiation rate of 58.6 ± 3.3%. Overall, this study provides a suitable 3D biomimetic platform to enable the efficient differentiation of stem cells into lung cells for lung tissue engineering.
研究领域:
组织工程
·
干细胞生物学
·
生物材料
·
肺再生
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2018
·Vol. 29
·期 2
·1806058
·
IF 13.325
Rui Chen,Jingjing Wang,Chen Qian,Yujie Ji,Chenqi Zhu,Li Wu,Weidong Li,Xiaolin Bi,Yutong Wang,Gang Cao,Zhipeng Chen
Peptide-drug conjugates (PDCs) are a type of self-assembled prodrug with good potential for drug delivery due to their excellent biocompatibility, high drug loading, and permanent controllable release. However, most PDCs tend to self-assemble into filamentous nanostructures in water and under physiological conditions, making them unsuitable as intravenous formulations due to the entanglement of long fibers and the risk of thrombus. Injected PDCs also face challenges in overcoming the complex physiological environment to reach the target site. To expand their clinical use, it is necessary to control the properties of PDC, including the self-assembled structure and physiological performance, to avoid the above problems. Based on assembly mechanism studies of PDCs, a new method for regulating PDC morphology is developed by controlling intermolecular interactions in the assembly process. This method can alter the final morphology of PDCs from nanofibers to nanorods, and the introduced macromolecules endow the PDC with new characteristics that facilitate stable and high-efficiency access to the target site.
研究领域:
药学
·
纳米技术
·
生物医学工程
·
癌症研究
✓ 已验证
DOI
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2011
·
IF 1.396
Jianian Chen,Shaoshun Li,Qi Shen,Huijuan He,Yi Zhang
The aim of this paper is to evaluate the cellular uptake of vincristine sulfate-loaded poly(lactic-co-glycolic acid)–polyethylene glycol (PLGA–PEG) nanoparticles with the folic acid modification (PLGA–PEG–folate NPs). PLGA–PEG–folate NPs were prepared using a water–oil–water emulsion solvent evaporation method. The particle size, surface morphology, drug encapsulation efficiency, and the drug release behavior were investigated. The NPs exhibited a biphasic drug release with a moderate initial burst followed by a sustained release profile. Internalization of the NPs labeled with coumarin- 6 by MCF-7 (Michigan Cancer Foundation-7) human breast cancer cells was quantitatively measured by microplate reader, and qualitatively analyzed by fluorescent microscopy and confocal laser scanning microscopy. The results showed PLGA–PEG–folate NPs achieved significantly higher cellular uptake in the folic acid receptor overexpressed MCF-7 cells, compared to PLGA–mPEG NPs without the folic acid modification. Due to the enhanced cellular uptake, PLGA–PEG–folate NPs displayed the highest cytotoxicity. Judged by IC50 after 24 h culture, the therapeutic effects of the drug formulated in the NPs with surface modification could be 1.52 times, 3.91 times higher than that of PLGA–mPEG NPs and free vincristine sulfate, respectively.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）