Theranostics
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2020
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IF 8.579
Weijie Ma,Daoming Zhu,Jinghua Li,Xi Chen,Wei Xie,Xiang Jiang,Long Wu,Ganggang Wang,Yusha Xiao,Zhisu Liu,Fubing Wang,Andrew Li,Dan Shao,Wenfei Dong,Wei Liu,Yufeng Yuan
Rationale : Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies in the world. Apart from traditional surgical resection, radiotherapy, and chemotherapy, more recent techniques such as nano-photothermal therapy and biotherapy are gradually being adopted for the treatment of HCC. This project intends to combine the advantages of nanoscale drug delivery systems with the targeting ability of CAR-T cells. Method : Based on cell membrane-coated nanoparticles and cell membrane-targeting modifications, a novel nanomaterial was prepared by coating CAR-T cell membranes specifically recognizing GPC3 + HCC cells onto mesoporous silica containing IR780 nanoparticles. Subsequently, the physical properties were characterized, and the in vitro and in vivo targeting abilities of this nanoparticle were verified. Results : CAR-T cells were constructed which could recognize GPC3 expressed on the cell surface of HCC cells. Then the isolated CAR-T cell membrane was successfully coated on the IR780 loaded mesoporous silica materials, as verified by transmission electron microscopy. The superior targeting ability of CAR-T cell membrane coated nanoparticles compared to IR780 loaded mesoporous silica nanoparticles was verified, both in vitro and in vivo . Conclusion : This new nanomaterial exhibits photothermal antitumor abilities along with enhanced targeting abilities, suggesting a promising strategy for the treatment of HCC.
研究领域:
肿瘤学
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纳米技术
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2022
·
IF 6.633
Shan Lu,Xuelin Dong,Binbin Zhang,Qi Zhou,Xinwei Zhang,Yonggui Liao,Yajiang Yang,Hong Wang
In this work, a dual-channel probe based on a complex of Eriochrome Black T (EBT) and Eu 3+ ions (denoted as EBT/Eu 3+ ) was used to sense four kinds of tetracyclines (TCs). UV–Vis and fluorescence spectra indicated that EBT/Eu 3+ molecule has the ability to emit dual channel signals of color and fluorescence. When TCs with different structures were added to the EBT/Eu 3+ solution, the color and the fluorescence intensities of EBT/Eu 3+ solution varied with TCs structure. FT-IR spectra revealed that a coordinating reaction occurred between acylamino groups, hydroxyl groups of A-ring in TCs and Eu 3+ ions. The coordinating ratio was 1: 2. Utilizing dual channel signals as well as the aid of principal component analysis (PCA), EBT/Eu 3+ was capable of discriminating not only single TCs with concentrations in nano-molar level, but also binary mixtures of TCs. The results of controlled experiments on various interfering substances and other antibiotics proved that this method has high selectivity for TCs detection. Furthermore, this two-channel sensor array based on EBT/Eu 3+ can also be used for the discrimination of unknown TCs in commercially available pork and honey with the 100% recognition accuracy. These results indicated that TCs with different structures can be rapidly discriminated by using a single and easily prepared sensor array.
研究领域:
分析化学
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传感器
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环境科学
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食品安全
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DOI
高影响因子
CANCER LETTERS
·
2021
·
IF 8.679
Ya-Nan Tan,Yong-Peng Li,Jian-Dong Huang,Min Luo,Shan-Shan Li,Anne Wing-Mui Lee,Fu-Qiang Hu,Xin-Yuan Guan
The response rate of anti-PD therapy in most cancer patients remains low. Therapeutic drug and tumor-infiltrating lymphocytes (TILs) are usually obstructed by the stromal region within tumor microenvironment (TME) rather than distributed around tumor cells, thus unable to induce the immune response of cytotoxic T cells. Here, we constructed the cationic thermosensitive lipid nanoparticles IR780/DPPC/BMS by introducing cationic NIR photosensitizer IR-780 iodide (IR780) modified lipid components, thermosensitive lipid DPPC and PD-1/PD-L1 inhibitor BMS202 (BMS). Upon laser irradiation, IR780/DPPC/BMS penetrated into deep tumor, and reduced cancer-associated fibroblasts (CAFs) around tumor cells to remodel the spatial distribution of TILs in TME. Interestingly, the cationic IR780/DPPC/BMS could capture released tumor-associated antigens (TAAs), thereby enhancing the antigen-presenting ability of DCs to activate cytotoxic T lymphocytes. Moreover, IR780/DPPC/BMS initiated gel-liquid crystal phase transition under laser irradiation, accelerating the disintegration of lipid bilayer structure and leading to the responsive release of BMS, which would reverse the tumor immunosuppression state by blocking PD-1/PD-L1 pathway for a long term. This combination treatment can synergistically exert the antitumor immune response and inhibit the tumor growth and metastasis.
研究领域:
肿瘤学
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免疫疗法
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纳米医学
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癌症研究
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DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2023
·
IF 5.6
Xin-Yu Shen,Jing-Mei Lu,Yu-Nan Lu,Guang-Nan Jin,Jing-Wen Ma,Jing-He Wang,Yu Wang,Xiang Xu,Lian-Xun Piao
Toxoplasma gondii ( T. gondii ) is an obligate intracellular protozoan parasite that causes pulmonary toxoplasmosis, although its pathogenesis is incompletely understood. There is no cure for toxoplasmosis. Coixol, a plant polyphenol extracted from coix seeds, has a variety of biological activities. However, the effects of coixol on T. gondii infection have not been clarified. In this study, we infected a mouse macrophage cell line (RAW 264.7) and BALB/c mice with the T. gondii RH strain to establish infection models in vitro and in vivo , respectively, to explore protective effects and potential mechanisms of coixol on lung injury caused by T. gondii infection. Anti- T. gondii effects and underlying anti-inflammatory mechanisms of coixol were investigated by real-time quantitative PCR, molecular docking, localized surface plasmon resonance, co-immunoprecipitation, enzyme-linked immunosorbent assay, western blotting, and immunofluorescence microscopy. The results show that coixol inhibits T. gondii loads and T. gondii -derived heat shock protein 70 ( T.g. HSP70) expression. Moreover, coixol reduced inflammatory cell recruitment and infiltration, and ameliorated pathological lung injury induced by T. gondii infection. Coixol can directly bind T.g. HSP70 or Toll-like receptor 4 (TLR4) to disrupt their interaction. Coixol prevented overexpression of inducible nitric oxide synthase, tumor necrosis factor-α, and high mobility group box 1 by inhibiting activation of the TLR4/nuclear factor (NF)-κB signaling pathway, consistent with effects of the TLR4 inhibitor CLI-095. These results indicate that coixol improves T. gondii infection-induced lung injury by interfering with T.g. HSP70-mediated TLR4/NF-κB signaling. Altogether, these findings suggest that coixol is a promising effective lead compound for the treatment of toxoplasmosis.
研究领域:
寄生虫学
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免疫学
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药理学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Rui Zhang,Mingrui Zhang,Qiao Ding,Ruo Yuan,Yali Yuan
In this work, a novel label-free and signal-off electrochemical biosensor based on efficient target-trigger T-structure recycling with dual strand displacement amplification (T-DSDA) was constructed for sensitive detection of miRNA-155 that related to cardiovascular diseases. Compared with traditional SDA, the proposed strategy not only accomplished simple polymerization and shearing of dsDNA without continuous consumption of temple DNA , but also ingeniously designed two analogous sequences that produced double cleavage sites, thereby obtaining extensive output DNA for further cleaving Y-structure assembled on electrode with significantly improved target conversion efficiency. Since the redox probe methylene blue (MB) attach to Y-structure via electrostatic interaction and tessellation, an obviously decreased current with a typical signal-off mode could be acquired for sensing miRNA-155 in the assistant of DNAzyme cleavage recycling. As a result, the electrochemical biosensor realized the rapid and sensitive detection of miRNA-155 with a low detection limit down to 166.7 fM. In addition, the biosensor displayed a strong anti-interference ability in bovine whole blood, and thus was expected to be applied to detect blood samples of various miRNA-155 related diseases in clinical diagnosis.
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2022
·Vol. 11
·期 2
·441-451
·
IF 7.571
Imran Mahmood Khan,Sobia Niazi,Imran Pasha,Muhammad Kashif Iqbal Khan,Lin Yue,Hua Ye,Ali Mohsin,Muhammad Shoaib,Yin Zhang,Zhouping Wang
Fluorescent gold (Au) nanostructures have emerged as burgeoning materials to fabricate nanomaterial assemblies which play a vital role in improving the detection sensitivity and specificity for various biomolecules. In this work, a fluorescence labelled (Rhodamine-B-Isothiocyanate) silica shell with Au metal core (AuNPs@PVP@RITC@SiO2) and a graphene–Au nanostar nanocomposite (rGO–AuNS) are presented as a metal enhanced fluorescence (MEF) material and Raman signal enhancer, respectively. Their composite (AuNPs@PVP@RITC@SiO2NPs/rGO–AuNS) was employed as a dual-mode fluorescence (FL) and surface-enhanced Raman scattering (SERS) nanoprobe for selective and sensitive detection of T-2 toxin. To comprehend the dual-modality, a core–shell nanostructure, AuNPs@PVP@RITC@SiO2, was functionalized with an aptamer (donor) and adsorbed on the surface of rGO–AuNS through electrostatic forces and π–π stacking which act as a FL quencher and SERS signal enhancer. When exposed to T-2 toxin, the apt-AuNPs@PVP@RITC@SiO2NPs move away from the surface of rGO–AuNS, resulting in the restoration of FL and reduction of the SERS signal. There was distinct linearity between the T-2 toxin concentration and the dual FL and SERS signals with lower limits of detection (LOD) of 85 pM and 12 pM, as compared to the previous methods, respectively. The developed FL and SERS aptasensor presented excellent recovery ratio and RSD in wheat and maize, respectively, as compared with the standard ELISA method. The complementary performances of the developed stratagem revealed a high correlation between the FL and SERS sensing modes with exquisite detection properties.
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2023
·
IF 2.6
Mingcheng Li,Xiaojie Liu,Chunying Zhu,Taotao Fu,Xiqun Gao,Youguang Ma
Densities and viscosities were measured for binary solutions MEA (monoethanolamine) + 3DMA1P (3-dimethylamino-1-propanol), 3DMA1P + H 2 O and ternary solutions MEA + 3DMA1P + H 2 O at P = 0.101 MPa, T = 293.15 K ∼ 333.15 K. Based on the experimental densities of solutions, the volume properties of the excess molar volume, partial molar volume, apparent molar volume and limiting partial molar volume were calculated. Moreover, the viscosity deviation and viscous flow activation energy were calculated based on the experimental viscosity. Additionally, the intermolecular interactions of the mixed solutions were analyzed and discussed, and the Redlich-Kister equation was used to correlate the excess molar volume and viscosity deviation of the binary solutions.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Jinhang Yang,Jiangli Sun,Shuo Chen,Danquan Lan,Zhihong Li,Zuji Li,Jingwen Wei,Zebin Yu,Hongxiang Zhu,Shuangfei Wang,Yanping Hou
Development of efficient and stable photocatalysts is of essential for photocatalytic degradation of refractory organics in wastewaters. Herein, the novel S-scheme MoSe 2 /AgBr (MS/AB) heterojunctions were synthesized for antibiotics degradation. Various characterization results showed that the S-scheme MS/AB photocatalysts exhibited efficient carriers’ separation and strong redox potential. Tetracycline (TC) (20 mg/L) degradation efficiency of 81.4% was achieved using the 5MS/AB within 90 min at pH of 9, superior to those of the counterparts. Photogenerated carriers' transfer and photocatalytic reaction mechanism of the S-scheme MoSe 2 /AgBr heterojunction were deduced by DFT calculation, in-situ XPS and work function calculation. Moreover, possible degradation pathways of TC were proposed based on identified intermediates, and most of the intermediates showed lower toxicity than that of TC. The 5MS/AB photocatalyst also showed great stability and excellent antibacterial effect. This study advances the design of efficient photocatalysts and enriches related mechanisms.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xiya Zhang,Mingyue Ding,Yexuan Mao,Xianqing Huang,Xinhua Xie,Lianjun Song,Mingwu Qiao,Jianwei Zhang,Tianlin Wang,Haihua Zhu,Zizhe Li,Youyi Wang,Meng Dang
The “turn-off” mode lateral flow immunoassay (LFA) based on the conventional competitive format and “turn-on” mode LFA mainly determined by inner filter effect (IFE-LFA) were objectively compared using T-2 toxin as a model molecule and T-2-BSA and anti-T-2 monoclonal antibody as immunocomplex pairs to improve the sensitivity of the LFA. First, Au nanoparticles (Au NPs), amorphous carbon nanoparticles (ACNPs), quantum dots nanospheres (QDs), and time-resolved fluorescent microspheres (TRFMs) were selected as labels for developing the “turn-off” mode LFAs (Au NPs-LFA, ACNPs-LFA, QDs-LFA, and TRFMs-LFA, respectively). Thereafter, Au NPs and ACNPs were used as absorbers, and QDs and TRFMs were selected as fluorescers, for preparing the “turn-on” mode IFE-LFAs (IFE-LFA based on Au NPs as absorbers and QDs as fluorescers [Au NPs-mAb-QDs-BSA], IFE-LFA based on Au NPs as absorbers and TRFMs as fluorescers [Au NPs-mAb-TRFMs-BSA], IFE-LFA based on ACNPs as absorbers and QDs as fluorescers [ACNPs-mAb-QDs-BSA], and IFE-LFA based on ACNPs as absorbers and TRFMs as fluorescers [ACNPs-mAb-TRFMs-BSA]). Under optimized conditions, the naked-eye observation cut-off values for the aforementioned eight LFAs were 4, 2, 2, 2, 3, 2, 1.5, and 1 ng/mL, with quantitative limit of detection values of 0.50, 0.23, 0.24, 0.23, 0.47, 0.45, 0.27, and 0.22 ng/mL, respectively. Among all LFAs, ACNPs-mAb-TRFMs-BSA showed the highest sensitivity. The results of this study provide a perspective for improving the sensitivity of the LFAs to meet the requirements of on-site screening for trace substances.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Qimin Peng,Xue Shao,Chuan Hu,Zuyang Luo,Tayirjan Taylor Isimjan,Zhifeng Dou,Ruobing Hou,Xiulin Yang
Developing the earth-abundant transition metal-based bifunctional electrocatalysts for water splitting and renewable energy devices has attracted much attention. Herein, we report a 1 T-WS 2 in ultrathin nanosheet arrays grafted with Co 4 S 3 on conductive carbon cloth (CC) (1 T-Co 4 S 3 -WS 2 /CC) through a feasible in-situ growth and vulcanization. The optimized 1 T-Co 4 S 3 -WS 2 /CC catalyst exhibits an impressive electrocatalytic activity and remarkable stability with the oxygen/hydrogen evolution reaction (OER/HER: 278/75 mV for 10 mA cm −2 ). It also showed the small Tafel slope values of 61.7 and 58.4 mV dec −1 , respectively. Additionally, the 1 T-Co 4 S 3 -WS 2 /CC (−/+) achieved 1.59 V@10 mA cm −2 in alkaline media superior to the most previously reported non-precious metal electrocatalysts. The outstanding performance could be attributed to the synergy between heterostructures of Co 4 S 3 and 1 T-WS 2 modifying the electronic structure to accelerate water splitting kinetics. Thus, this work presents a rational design of scalable, high-efficiency, stable water splitting electrocatalysts based on WS 2 .
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PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 7
·9032-9043
·
IF 2.478
Liu Yunyi,Pan Yongqi,Bai Xudong,Wang Jinchuan,He Caiwang,Xu Zunping,Chen Yi
A conventional ceramic preparation method was used to fabricate the newly designed ternary (K,Na)NbO 3 -based lead-free ceramics, which have the general formula of (0.97- x )K 0.40 Na 0.60 Nb 0.95 Sb 0.05 O 3 –0.03Bi 0.5 K 0.5 HfO 3 – x SrZrO 3 . An X-ray diffraction analysis, together with dielectric-temperature measurements, was performed for determining their phase structures. The crystal structure was found to transition from a rhombohedral-orthogonal phase coexistence to a three-phase coexistence of rhombohedral-orthogonal-tetragonal phases with the increase in SrZrO 3 concentration, and then to a rhombohedral-tetragonal phase coexistence, and finally to a single rhombohedral phase. Near the three-phase coexistence zone, the ceramics exhibit an obviously enhanced electrical activity, with an electromechanical coupling coefficient k p = 0.364 and a piezoelectric constant d 33 = 280 pC/N. Through a microstructure analysis, a slightly excessive amount of SrZrO 3 was found to result in an inhibition for the grain growth of the studied ceramics. These results show that incorporating SrZrO 3 into ternary (K,Na)NbO 3 -based ceramics is still an effective means of constructing phase boundaries and improving the electromechanical properties.
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高影响因子
AICHE JOURNAL
·
2021
·Vol. 67
·期 8
·e17252
·
IF 3.993
Hu Li,Zisheng Zhang,Guanlun Sun,Suli Liu,Liangcheng An,Xingang Li,Hong Li,Xin Gao
As an attractive alternative technology for the separation of long chain olefin and paraffin, a novel silver-based deep eutectic solvent (Ag-DES) was prepared and utilized for 1-octene/n-octane separations. Comprehensive reactive extraction separation experiments were performed to highlight the Ag-DES concentration and operating temperature discriminations using compounds with different ratio of 1-octene/n-octane. The novel Ag-DES showed optimal separation performance regarding 1-octene/n-octane and possessed the highest levels separation selectivity in the range 3.57–16.11 with excellent circulation stability in our best knowledge. Furthermore, FT-Raman measurements and quantum chemistry calculation were performed to elucidate the interaction mechanism of Ag-DES in the separation of 1-octene and n-octane, which revealed that both chemical complexation and strong physical attraction existed in the complex of Ag-DES and 1-octene rather than n-octane. A practical process was proposed for the separation of olefin and paraffin, which indicates that an advanced separation technology could largely reduce the energy consumption. This study lends important insight for the development of Ag-DES reactive extraction separation process for the energy-efficient long chain α-olefins purification from F-T synthesis products.
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高影响因子
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