Scientific Reports
·
2016
·Vol. 6
·期 1
·1-15
·
IF 5.228
Zhang Tingjing,Liang Jianqiang,Wang Panxue,Xu Ying,Wang Yutang,Wei Xinyuan,Fan Mingtao
Phloretin-2′- O -glycosyltransferase (P2′GT) catalyzes the last glycosylation step in the biosynthesis of phloridzin that contributes to the flavor, color and health benefits of apples and processed apple products. In this work, a novel P2′GT of Malus x domestica (Md P2′GT) with a specific activity of 46.82 μkat/Kg protein toward phloretin and uridine diphosphate glucose (UDPG) at an optimal temperature of 30 °C and pH 8.0 was purified from the engineered Pichia pastoris broth to homogeneity by anion exchange chromatography, His-Trap affinity chromatography and gel filtration. The purified Md P2′GT was low N -glycosylated and secreted as a stable dimer with a molecular mass of 70.7 kDa in its native form. Importantly, Md P2′GT also exhibited activity towards quercetin and adenosine diphosphate glucose (ADPG), kaempferol and UDPG, quercetin and UDP-galactose, isoliquiritigenin and UDPG, and luteolin and UDPG, producing only one isoquercitrin, astragalin, hyperoside, isoliquiritin, or cynaroside, respectively. This broad spectrum of activities make Md P2′GT a promising biocatalyst for the industrial preparation of the corresponding polyphenol glycosides, preferably for their subsequent isolation and purification. Besides, Md P2′GT displayed the lowest K m and the highest k cat /K m for phloretin and UDPG compared to all previously reported P2′GTs, making Md P2′GT favor phloridzin synthesis the most.
研究领域:
植物学
·
生物化学
·
食品科学
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2026
·Vol. 37
·期 3
·192
·
IF 2.8
Ge Shaofan,Yang Yujie,Ding Hongyu,Geng Zhihao,Hu Tan
Magnetic loss is a key factor limiting the application of nanocrystalline soft magnetic composite materials (n-SMCs) in high-frequency environments, and insulative coating and annealing treatment are important methods to reduce magnetic loss. In this study, a nano-La₂O₃ insulative coating was applied to the surface of FeSiBNbCu nanocrystalline magnetic powder via physical grinding combined with ultrasonic vibration. The effects of nano-La₂O₃ coating content (0–1 wt%) and annealing temperature (455–530 ℃) on the soft magnetic properties of the magnetic powder core were systematically investigated. The results showed that the nano-La₂O₃ coating could significantly improve the insulative performance between magnetic powders and effectively reduce the P e of n-SMCs. With the increase of nano-La₂O₃ content, the P e showed a trend of first decreasing and then increasing, reaching the lowest at 0.75 wt%. The P e decreases from 193.43 mW/cm 3 (with 0.0 wt% La₂O₃) to 142.537 mW/cm 3 (with 0.75 wt% La₂O₃ at f = 1 MHz), representing a reduction of 26.3%. Meanwhile, by optimizing the annealing process, the residual internal stress in the magnetic powder core induced during compaction is effectively relieved, leading to a significant decrease in hysteresis loss. As the annealing temperature increases, the hysteresis loss of the magnetic powder core reaches its minimum at 505 °C. When the nano-La₂O₃ content was 0.75 wt% and the annealing temperature was 505 ℃, the prepared nanocrystalline magnetic powder core exhibited optimal soft magnetic properties: a stable effective permeability (22.47) and the lowest total loss (230.4 mW/cm 3 at f = 1 MHz, B m = 20 mT), which was 22.89% lower than that of the untreated sample. This study provides a new process scheme for the development of high-performance low-loss n-SMCs.
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Science China-Physics Mechanics & Astronomy
·
2018
·Vol. 61
·期 9
·1-4
·
IF 2.754
Zhang Yue,Liu Wenhao,Si Jin,Zhu Xiyu,He Chengping,Zhao Haonan,Wen Hai-Hu
Superconductivity is observed in a new nontoxic cuprate system Ga-Ba-Ca-Cu-O, with T c = 82 K for GaBa 2 Ca 5 Cu 6 O 14 + δ ( 5 (Ga-1256) and T c = 116 K probably for GaBa 2 Ca 3 Cu 4 O 10+δ (Ga-1234) or GaBa 2 Ca 2 Cu 3 O 8+δ (Ga-1223), respectively. All compounds are fabricated by solid state reaction method under high pressure and high temperature. Samples are characterized by resistivity, magnetization and X-ray diffraction (XRD) measurements. The temperature dependence of magnetization measured in both zero-field-cooled and field-cooled processes on one sample (S1) shows two superconducting transitions at about 82 K and 113 K. The estimated shielding fraction for the phase with T c of 82 K is about 67%, while the fraction for another phase with T c of 113 K is quite small. The XRD Rietveld refinement for S1 indicates two main phases existing in the sample, Ga-1256 with fraction of about 58% and non-superconducting Ca 0.85 CuO 2 with fraction of about 42% respectively. Thus, we can conclude the superconducting phase with transition temperature of 82 K is due to Ga-1256. The resistivity measurement also confirms the superconductivity for S1, and the resistivity reaches zero at about 82 K. The temperature dependence of magnetization for another sample (S2) shows much higher superconducting shielding fraction for the phase with T c of 116 K, which may be a promising prospective for the synthesis of Ga-1234 or Ga-1223 phase.
研究领域:
凝聚态物理
·
超导物理
·
材料科学
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MATERIALS CHARACTERIZATION
·
2013
·
IF 1.88
Weimin Du,Guoyan Zhao,Hongxun Chang,Fei Shi,Zhaoqiang Zhu,Xuefeng Qian
With the help of sol–gel method assisted by melting salt, a series of Ho–Zr–O nano-composite with controllable composition and defects have been successfully prepared. Characterization results show that the positions, intensity, and width of the X-ray diffraction peaks of the products have a regular variation with the increase of zirconium element which implies the gradual changes of crystal spacing and product size. At the same time, the molar ratios between holmium and zirconium ions are consistent with the chemical formula and both of them are uniformly distributed in products further showing the perfect formation of targeted materials. Optical properties reveal that diversified defect forms of Ho–Zr–O nano-composite lead to the different absorptions of visible light . Photocatalytic experiments demonstrate Zr 0.8 Ho 0.2 O 2 − δ nano-crystals have excellent visible-light-responsive photocatalytic activities on some familiar dyes (e.g.: methylene blue and Rhodamine B) which results from the special defect structure , better absorption of visible light and larger specific surface area . It follows that Zr 0.8 Ho 0.2 O 2 − δ nano-crystals are a new kind of visible-light-responsive photocatalysts with better prospects in conversion and utilization of solar energy. Also, the present melting salt assisted route might be generalized to synthesize other AxByOz composite oxide nano-crystals with more complicated structures.
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JOURNAL OF ORGANOMETALLIC CHEMISTRY
·
2021
·
IF 2.369
Ji Wu,Ke Cao,Cai-Yan Zhang,Xin-Yu Wen,Bo Li,Junxiao Yang
A FeCl 3 catalyzed tandem condensation/cyclization/aerobic oxidation process for synthesis of 1-benzoxazolyl- o -carboranes has been developed. The degradation of o -carborane in the presence of aza-/oxa-nucleophiles was completely suppressed, and a series of 1-benzoxazolyl- o -carboranes, 1-benzothiazolyl- o -carborane and 1-benzimidazolyl- o -carborane have been synthesized with good to excellent yields. This work offers a general protocol for synthesis of 1-benzoxazolyl- o -carboranes, which has important reference for synthesis of aromatic heterocycle-carborane derivatives and promote its applications in miscellaneous areas.
研究领域:
有机化学
·
硼化学
·
催化化学
·
杂环化学
·
材料化学
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ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Tong-Mou Geng,Deng-Kun Li,Zong-Ming Zhu,Wei-Yong Zhang,Sai-Nan Ye,Hai Zhu,Zhu-Qing Wang
A novel conjugated microporous polymer based on perylene tetraanhydride bisimide (DP 2 A 2 ) has been synthesized through Sonogashira–Hagihara cross-coupling polymerization of tetrabromo-substituted perylene tetraanhydride bisimide derivative (DPBr 2 ABr 2 ) with 1,4-diethynylbenzene, whose Brunauer–Emmett–Teller (BET) specific surface area is about 378 m 2 g −1 . The fluorescence quenching behaviors of the DP 2 A 2 were investigated. It is found that the DP 2 A 2 shows high sensitivity and selectivity to tracing o-nitrophenol (o-NP) in THF with Ks V constant of 2.00 × 10 4 L mol −1 . The detection limit (LOD) is 1.50 × 10 −9 mol L −1 . The possible sensing mechanism for the luminescent quenching of DP 2 A 2 towards o-NP exciting at 365 nm was considered the donor–acceptor electron transfer mechanism, which is a combined result from both dynamic (collisional) and static quenching. Moreover, the static quenching process is dominant for DP 2 A 2 .
研究领域:
材料科学
·
分析化学
·
聚合物化学
·
传感器与传感技术
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Micro & Nano Letters
·
2020
·Vol. 15
·期 3
·125-129
·
IF 0.975
Chulei Zhao,Kaihang Cao,Chaoyun Ma,Wenjun Li,Yiju Song,Xiuwen Qiao,Chenglin Hong
In this experiment, a Cu 2 O spherical nanoparticle was reduced by hydrothermal reaction to prepare a label-free electrochemical immunosensor based on a novel signal amplification strategy. Multi-walled carbon nanotubes are a platform that combines cuprous oxide with carbon nanotubes. Nanogold was modified on the surface of cuprous oxide nanospheres by electrostatic adsorption to further amplify the electrochemical signal and link-supported anti-alpha-fetoprotein (AFP). Under optimal conditions, the proposed immunosensor has a wide linear range from 0.001 to 40 ng/ml, with a detection limit as low as 0.33 pg/ml ( S / N = 3). Furthermore, the proposed label-free immunosensor has been used to determine AFP in human serum with satisfactory results. At the same time, it has good reproducibility, acceptable selectivity and long-term stability, and has broad application prospects in biometric analysis.
研究领域:
分析化学
·
纳米科技
·
生物化学
·
生物医学工程
·
电化学
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Journal of Energy Storage
·
2026
·
IF 9.8
Fanen Zeng,Bing Xu,Zihao Zhang,Qinyi Yang,Yang Sun,Chunyang Li,Guojun Li
High–entropy oxides (HEOs) are promising electrode materials for supercapacitors because of their tunable compositions and structural stability. However, conventional synthesis routes often involve the differences in metal ion diffusion rates that result in particle coarsening and agglomeration, which limit the full realization of electrochemical performance. Here, a surfactant–assisted hydrothermal method using cetyl trimethyl ammonium bromide (CTAB) as a structural template was employed to synthesize a porous (CrMnFeCoNi)₃O₄ high–entropy spinel oxide. Uniform and well–dispersed nanoparticles with abundant mesopores were obtained by adjusting the molar ratio of CTAB/metal cations (C/M). The optimized sample (C/M = 1.0) exhibited a specific capacitance of 570.6 F g −1 at 0.5 A g −1 and retained 75.36 % capacitance at 10 A g −1 . Kinetic analysis indicated that capacitive behavior dominated the charge storage process. An asymmetric supercapacitor, assembled with the produced HEO as the positive electrode and activated carbon (AC) as the negative electrode, exhibited an energy density of 54.4 Wh kg −1 at a power density of 562.4 W kg −1 , with 77.77 % capacitance after 10,000 cycles. This study demonstrates a simple and effective strategy to prepare porous HEOs used for high–performance supercapacitors.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Yi Kuang,Cailing Tan,Xianrui Tan,Jian Zhang
Isoflavones from legumes, particularly genistein, are highly valued as functional food ingredients. However, the poor water solubility and low bioavailability hinder its application, which can be improved through glycosylation. The glycosyltransferase GT2419 from Bacillus subtilis ATCC 6633 catalyzes the 7-OH and 4′–OH glycosylation of genistein, but its poor regioselectivity hampers the efficient synthesis of genistein 7-O-glucoside. Here, a semirational design strategy was employed to obtain the F231V mutant, which significantly increased the conversion rate of genistein 7-O-glucoside from 21.1% to 72.2% and improved the 7-O regioselectivity to 73.8%. Kinetic analysis revealed that the F231V mutant exhibited enhanced affinity and catalytic efficiency toward genistein. Computational analysis revealed that the F231V mutant enlarged the substrate-binding pocket and disrupted intermolecular hydrophobic interactions, thereby altering substrate orientation, and enhancing the regioselective formation of genistein 7-O-glucoside. This study provides an efficient biosynthetic method for genistein 7-O-glucoside, with promising applications in the food industry.
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Journal of Physical Chemistry Letters
·
2026
·Vol. 17
·期 27
·7770-7779
·
IF 4.5
Sun Tianjiao,Xu Shuting,Ma Jing,Yu Zhiwu
There
exist two pathways in the cleavage of ester bonds, R–(O═)C–O–C–R’,
which are central to polyester recycling and organic synthesis. Traditional
views emphasize electronic effects, where the more positively charged
carbon is considered the preferred site for nucleophilic attack, resulting
in the breaking of C acyl –O bond. However, halide
based ionic liquids were found to selectively cleave the C alkoxy –O bond of polyesters, a finding that cannot be rationalized
by charge considerations alone. In this work, we propose that it is
the presence of clusters in solutions that leverages the accessibility
of reactants and thus determines the reaction pathways. The idea has
been demonstrated in the study of a model binary system of methyl
benzoate (MB) and 1-butyl-3-methylimidazolium bromide ([BMIm]Br) using
excess infrared spectroscopy, molecular dynamics simulations, and
density functional theory calculations. Excess infrared spectroscopy
reveals seven distinct aggregate species in solution, including ionic
liquid-ester clusters and MB self-aggregates, providing direct experimental
evidence that the solution is microheterogeneous and that cluster
formation dictates the local reaction environment. In the catalytically
relevant cluster, [BMIm]Br(MB) 3 , DFT optimized geometries
show that the Br – ···C alkoxy distance (3.56 Å) is significantly shorter than the Br – ···C acyl distance (5.53 Å).
Molecular dynamics simulations confirm the preferential solvation
of C alkoxy around Br – . A potential energy
surface scan with respect to the Br – ···C
distance identifies a critical distance of approximately 3.5 Å
where the alkoxy C–O bond begins to deviate from equilibrium,
marking the incipient stage of partial bonding. At 2.34 Å, the
C alkoxy –O bond undergoes abrupt elongation, corresponding
to an energy maximum, showing a pattern of the transition state in
classic S N 2 reactions. These findings establish the proximity
effect, through cluster mediated spatial preorganization, as the governing
principle of C–O bond cleavage selectivity in ionic liquid-ester
systems.
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Journal of the American Chemical Society
·
2026
·Vol. 148
·期 29
·31331-31342
·
IF 16.6
Sun Yuwen,Gong Kun,Li Tianyu,An Yunlei,Sun Qiwen,Lin Tiejun,Zhong Liangshu
Nonoxidative
ethane dehydrogenation (NOEDH) is a carbon
efficient
but thermodynamically severe route for ethene production. Solar-driven
NOEDH offers a promising alternative, yet achieving both high activity
and high ethene selectivity over noble metal-free catalysts without
external heating remains unresolved. Here, we reported that Ti 3+ -O acid–base pairs on reduced rutile TiO 2 enabled highly efficient full-spectrum solar-driven photothermal
NOEDH without any external heating. The optimally reduced TiO 2 at 750 ° C delivered an ethene production rate of 392.0
mmol g –1 h –1 , which was 1107 times
higher than that of pristine TiO 2 without reduction pretreatment.
The high activity with a C 2 H 4 selectivity of
94.9% surpassed the thermodynamic equilibrium limit and outperformed
previously reported systems. Comprehensive characterizations identified
surface Ti 3+ sites adjacent to lattice oxygen as Lewis
acid–base pairs that enhanced light absorption, promoted charge
carrier separation, and directly participated in the C–H bond
cleavage. Photogenerated electrons also preferentially accumulated
on the coordinatively unsaturated Ti 3+ -O sites, which weakened
ethene adsorption, thereby accelerating product desorption and suppressing
deep dehydrogenation and coke formation. The synergy between photogenerated
hot carriers and localized photothermal heating lowered the apparent
activation energy from 157.6 kJ mol –1 in the dark
to 75.0 kJ mol –1 under illumination. This work establishes
surface acid–base pairs on noble metal-free catalyst as a powerful
platform for sustainable light-driven alkane upgrading.
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AICHE JOURNAL
·
2026
·
IF 4.4
Chi Wang,Juncheng Wu,Yi Cheng,Jinshu Tian,Chong Hao,Guan Sheng,Lingkun Wu,Yonghe Li,Zailai Xie
Supported boron oxide catalysts show great potential in propane oxidative dehydrogenation yet suffer severe hydrolytic deactivation at high temperatures. Here, an interfacial bond gradient strategy is proposed to stabilize boron-based catalysts. CeO 2 anchored on γ-Al 2 O 3 modulates local bonding and firmly immobilizes active boron species. The catalyst achieves over 40% propane conversion, 80% olefin selectivity and superior stability within 200 h. Formed B-O-Ce interfaces trigger oriented electron transfer and enhance surface hydrophobicity, raising hydrolysis activation energy and suppressing active site loss. This interfacial engineering offers a universal route to design robust catalysts for alkane transformation.
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