INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Lin Gao,Luping Gu,Wentian Li,Gang Wang,Jianxin Zhao,Wei Chen,Peijun Tian
5-Hydroxytryptophan (5-HTP) is a functional food ingredient widely recognized for its sleep-enhancing properties, but it has a short elimination half-life in vivo , resulting in suboptimal pharmacokinetic performance. In this study, we developed a composite nanoparticles system based on β-cyclodextrin and corn starch using the anti-solvent precipitation method for the encapsulation and sustained release of 5-HTP. The nanoparticles were characterized using laser particle size analysis, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and evaluation of their digestive properties in simulated gastrointestinal fluids. We further evaluated their effects on the pharmacokinetic profile and distribution of 5-HTP in rats, including serum, gut, and brain, demonstrating a significant extension of the drug elimination half-life and improved bioavailability. In an insomnia mouse model, administration of the composite nanoparticles system significantly alleviated behavioral abnormalities and circadian rhythm disorder induced by sleep deprivation. This study provides a solid theoretical basis and technical support for the efficient application of 5-HTP in functional foods.
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高影响因子
Nanoscale
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2017
·Vol. 9
·期 18
·5859-5871
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IF 7.367
Hao Zhao,Jiabao Xu,Jiangshan Wan,Shinan Geng,Han Li,Xiaole Peng,Qianwen Fu,Ming He,Yanbing Zhao,Xiangliang Yang
To realize the sustained release and long-term intratumoural retention of water-soluble cisplatin, thermo/pH-sensitive cisplatin-directed coordination-crosslinking nanogels (Pt-PNA) were developed via the coordination bonds of Pt–carboxyl groups. As the coordination ratio (CR) of the Pt–carboxyl bonds increased from 5% to 35%, the sizes of the Pt-PNA nanogels decreased from 999 nm to 167 nm, and their zeta potentials increased from −35 mV to −13 mV. Only through a simple mixing of cisplatin and PNAs, the entrapment efficiencies (EEs) of the Pt-PNA nanogels reached near 100% (>90%), and the drug-loading amounts (DLs) of cisplatin could achieve up to 25.5 ± 0.1%. For water-soluble cisplatin, Pt-PNA nanogels exhibited a sustained release for as long as 5 days. The thermo/pH-sensitive sol–gel phase-transition behaviour of the Pt-PNA nanogels were investigated via inverting-vial and rheological methods. Platinum elemental analysis indicated that the Pt-PNA nanogels showed a much stronger ability of cisplatin retention in tumours than free cisplatin. The platinum content in a tumour treated by the Pt-PNA nanogels was far higher than that by free cisplatin: 200.7 ± 63.6 μg vs. 82.7 ± 26.8 μg at the 1st day, or 118.9 ± 35.2 μg vs. 18.5 ± 9.4 μg at the 14th day. The evaluation of the in vivo antitumour efficacy indicated that only after a single dose of Pt-PNA nanogels, the tumour volume continuously decreased to 0.73 ± 0.07 times that of the original tumour volume (OTV) for 14 days; however, it rapidly increased by 3.37 ± 0.82, 8.01 ± 0.53 and 9.25 ± 1.85 times that of the OTV with the same dose of free cisplatin, PNA, and NS, respectively. Some preliminary evaluations of the biocompatibility indicated that the toxic side effects of cisplatin could be greatly improved via cisplatin-directed coordination-crosslinking with PNA. As a result, Pt-PNA nanogels could likely become a promising versatile strategy for improving antitumour efficacy and reducing the toxicity and size effects of platinum-based drugs, and they could also be developed as promising nanomedicines for regional chemotherapy.
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BIOMATERIALS
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2016
·
IF 8.387
Xiaofeng Shen,Yanxia Zhang,Yong Gu,Yun Xu,Yong Liu,Bin Li,Liang Chen
In this study, a cell-free bone tissue engineering system based on a silk fibroin (SF)/nano-hydroxyapatite (nHAp) scaffold was developed, in which two bioactive molecules , stromal cell derived factor-1 (SDF-1) and bone morphogenetic protein-2 (BMP-2), were embedded and released in a sequential and controlled manner to facilitate cell recruitment and bone formation, respectively. BMP-2 was initially loaded into SF microspheres , and these BMP-2 containing microspheres were subsequently encapsulated into the SF/nHAp scaffolds, which were successively functionalized with SDF-1 via physical adsorption. The results indicated rapid initial release of SDF-1 during the first few days, followed by slow and sustained release of BMP-2 for as long as three weeks. The composite scaffold significantly promoted the recruitment of bone marrow mesenchymal stem cells (BMSCs) and osteogenic differentiation of them in vitro . Further, the in vivo studies using D-Luciferin-labeled BMSCs indicated that implantation of this composite scaffold markedly promoted the recruitment of BMSCs to the implanted sites. Enhanced bone regeneration was identified at 12 weeks' post-implantation. Taken together, our findings suggested that the sequential and sustained release of SDF-1 and BMP-2 from the SF/nHAp scaffolds resulted in a synergistic effect on bone regeneration. Such a composite system , therefore, shows promising potential for cell-free bone tissue engineering applications .
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International Journal of Nanomedicine
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2014
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IF 4.195
Yun Wang,Fu-xing Lin,Yu Zhao,Mo-zhen Wang,Xue-wu Ge,Zheng-xing Gong,Dan-dan Bao,Yu-fang Gu
Novel submicron core-shell-structured chitosan-based composite particles encapsulated with enhanced green fluorescent protein plasmids ( pEGFP ) were prepared by complex coacervation method. The core was pEGFP -loaded thiolated N-alkylated chitosan (TACS) and the shell was pH- and temperature-responsive hydroxybutyl chitosan (HBC). pEGFP -loaded TACS-HBC composite particles were spherical, and had a mean diameter of approximately 120 nm, as measured by transmission electron microscopy and particle size analyzer. pEGFP showed sustained release in vitro for >15 days. Furthermore, in vitro transfection in human embryonic kidney 293T and human cervix epithelial cells, and in vivo transfection in mice skeletal muscle of loaded pEGFP , were investigated. Results showed that the expression of loaded pEGFP , both in vitro and in vivo, was slow but could be sustained over a long period. pEGFP expression in mice skeletal muscle was sustained for >60 days. This work indicates that these submicron core-shell-structured chitosan-based composite particles could potentially be used as a gene vector for in vivo controlled gene transfection.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Daibing Hou,Xuedan Cui,Yiming Tang,Meng Liu,Hantong Qie,Pengjie Zhao,Wenpeng Leng,Nan Luo,Huilong Luo,Wenxia Wei,Aijun Lin,Tianwen Zheng
Rebounding and lagging during the process of remediating contaminated sites are challenging problems. A potential solution is developing slow-release materials that could assist in the long-term remediation of contaminated sites. This study synthesised a slow-release Fe-C composite (CSBC-nZVI@β-CD), which exhibits a synergistic effect of encapsulated layers and nanocracks, using a liquid-phase reduction method. Furthermore, the reduction and dechlorination mechanism of trichloroethene (TCE) was systematically investigated. The introduction of β-cyclodextrin (β-CD) intensified the Kirkendall effect, forming nano zero-valent iron (nZVI) with abundant radial nanocracks and a litchi-like appearance on the biochar surface. Additionally, β-CD created an encapsulation layer measuring 5–7 nm on the surface of nZVI, improving its electron transport capacity and hydrophobic properties. The CSBC-nZVI@β-CD nanocomposite successfully removed 99 % of TCE in solution within 2 h and accomplished a degradation efficiency of 98.9 % within 15 d. The synergistic effect of the β-CD encapsulating layer and nanocracks facilitated the reactivity and electron-retarding capacity, resulting in a broad pH tolerance and anti-interference ability. Density-functional theory (DFT) calculations and experimental methods were used to study the mechanism of TCE reduction dechlorination; the unique structure of β-CD tended to bind with Fe as well as adsorb TCE, thereby facilitating electron transfer and the enrichment of TCE at the reaction interface. In addition, the sustained-release Fe-C composites reduced the bonding energy required during the TCE degradation reaction and promoted the complete reaction of the product. Overall, CSBC-nZVI@β-CD exhibited significant potential for the long-term remediation of groundwater.
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CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Hongfa Duan,Xiaochi Liu,Lulu Bian,Zhongyi Li,Ning Li,Muhammad Umair Sial,Zhe Sun,Haiqiang Gao,Beixing Li,Daxia Zhang,Feng Liu
The preparation of pesticide-loaded microcapsules using biodegradable polymers and the balance between their sustained-release performance and small-size effects represent an effective strategy to enhance pesticide utilization efficiency. This study devised a one-step sol-gel self-assembly approach to fabricate abamectin-loaded microcapsules using biodegradable polyvinyl alcohol (PVA) and butylated hydroxytoluene (BHT) as raw materials. The results demonstrated that the reaction through hydrogen bonding by PVA and BHT at the o/w interface could generate shells and encapsulate pesticides to make microcapsules. Increasing PVA usage could reduce the particle size and enhance shell stability. The addition of BHT also improved the shell structure stability and encapsulation efficiency, whereas 3 %–7 % concentrations impeded the formation of the shell structure. Abamectin, which has a self-polymerizing ability, also increases microcapsule stability. A 54 °C temperature increase was maintained for 24 h to induce emulsion separation. Subsequent cooling and agitation prompts the reconstitution of pesticide-loaded microcapsules. The synergistic effect of the water-responsive mechanism, high encapsulation efficiency, and thicker shells endows nano-formulations with superior sustained-release performance compared to micro-scale formulations. The photostability of abamectin nanocapsules is significantly enhanced by 68.26 % compared to conventional suspensions, and the content of active ingredients remains at 50.54 % after 60 h of release under light conditions. Owing to their nanoscale sustained-release characteristics, their biological safety is improved by 36.43 %. The encapsulated Aba@BHT-PVA nano-system, leveraging excellent rainwater erosion resistance and leaf surface affinity, significantly prolongs its leaf retention time. Synergizing with the small-size effect, it demonstrates substantial enhancement in both the control efficacy and persistence against Plutella xylostella . Owing to its unique properties and easy regulation, this fabrication technique has great application value in the future.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Shuqi Guo,Xueze Zhang,Shangxuan Li,Huifang Wang,Xianghua Gao,Baolong Niu,Wenfeng Li
Quercetin (Que) is a naturally occurring bioactive compound with various potential health benefits, but its low stability and low bioaccessibility limit its application in functional foods. Therefore, there is an urgent need to prepare a novel composite hydrogel bead with efficient and controlled release. Specifically, porous starch (PS) was first prepared by enzymatic hydrolysis to increase the specific surface area and thus enhance the adsorption rate of Que. Scanning electron microscopy confirmed the formation of the PS and the adsorption of Que. The maximum swelling properties of carboxymethylcellulose-porous starch composite hydrogel beads (CQPZ) were reduced by adding different amounts of zinc oxide to achieve slow release. The results showed that the higher the amount of zinc oxide, the stronger the slow-release performance, while the lower the drug loading capacity. To obtain composite hydrogel beads with both low swelling and high drug-carrying properties, bilayer gel beads (CQPZ/CS) were prepared by electrostatic self-assembly of carboxymethyl cellulose and chitosan. The experimental results showed that the drug loading was significantly increased by nearly 2-fold while enhancing the slow-release performance. In addition, the CQPZ/CS composite hydrogel beads enhanced the stability of Que under light and heat conditions, and the DPPH radical scavenging rate after UV irradiation was still greater than 75%, which was much higher than that of free quercetin (31.08%). In conclusion, the prepared hydrogel beads are a promising delivery system for bioactive compounds.
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Journal of Advanced Research
·
2025
·
IF 13
Jin-Long Tian,Nuo Wang,Qin-Fu Zhao,Zhi-Huan Zang,Zhi-Ying Li,Zhen-Yu Wang,Ying Zhou,Bao-Ru Yang,Sergey S. Makarov,Anton I. Chudetsky,Liang Wang,Ying He,Bin Li
Introduction Anthocyanins have various health benefits but are limited by fast elimination and low bioavailability. Many studies have shown that nano delivery systems are effective in improving the intestinal absorption and bioavailability of anthocyanins. However, in the process of selecting anthocyanin nanomaterials, a dilemma is faced: numerous functional nanomaterials lack edible properties, it is difficult to meet the edible and functional requirements of nanomaterials simultaneously. Therefore, it is necessary and inevitable to select nano-anthocyanin carriers that are edible and have certain functional and morphological characteristics. Objectives We aim to provide an efficient, sustained-release and easy to industrialize nanosystem method to solve the problem of low bioavailability of anthocyanins. Methods A succinylated casein-phospholipid-anthocyanin nanosystem (CLS) was designed via computational chemistry-guided one-step self-assembly. Morphological, in vitro/in vivo digestion, pharmacokinetic were conducted. Furthermore, the effects of CLS on intestinal microecology were verified by using enteritis model animals, and the lung-targeting effect of CLS on lung health was verified by using lung injury model animals. Results CLS exhibited 24-hour intestinal sustained release and lung-targeted enrichment. Pharmacokinetic studies showed 25.7-fold higher lung AUC (0-t) and 13.1-fold higher blood AUC (0-t) compared to free anthocyanins. CLS significantly alleviated lung injury and colitis in model rats via the gut-lung axis. Conclusion This study significantly improved the bioavailability of anthocyanins and demonstrated the oral lung-targeting function of anthocyanins for the first time, paving the way for utilizing anthocyanins as dietary supplements and precision nutrition.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Ke Liu,Jinghe Sun,Xinting Zhang,Chaohui Wang,Jingfeng Yang
To extend shrimp shelf life during storage, a composite film incorporating cinnamon essential oil (CEO) was developed, composed of oxidized hyaluronic acid and thiol-modified carboxymethyl chitosan. The results showed that the CEO was stably encapsulated within the composite film (OSC) and formed OSC-CEO, and its elongation at break and puncture strength increased to 59.83 % and 5.89 kg/mm 2 , respectively. Meanwhile, the film loaded with 7 % CEO achieved a water vapor permeability of 7.14 × 10 −12 g/m·s·Pa and an oxygen permeability of 16 × 10 −9 g/m 2 s. Under reducing conditions, the cleavage of disulfide bonds in the film reduces the network density and accelerates the release of CEO. Moreover, the film containing 7 % CEO achieved an effective loading rate of 80.53 % and maintained sustained release over 42 h. This controlled-release mechanism results in potent antibacterial activity and oxidation resistance. Compared with the unpackaged group, the 7 % CEO treatment group exhibited better preservation during the shrimp storage. The OSC-7 % CEO film-coated shrimp showed a weight loss (10.54 %), TVB-N content (27.55 mg/100 g), MDA content (0.51 mg/100 g), TVC (4.88 log CFU/g), and ORP (−49.51 mV). This novel composite material offers a new approach to active food packaging, and it has broad applications in shrimp storage.
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POLYMERS FOR ADVANCED TECHNOLOGIES
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2020
·Vol. 32
·期 3
·1262-1271
·
IF 2.578
Qingzhen Zhu,Baogang Zhang,Yuandou Wang,Xinghua Liu,Weiwei Li,Feng Su,Suming Li
Poly( D , L -lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) diblock copolymers with different compositions were synthesized by ring-opening polymerization of D , L -lactide and glycolide, using monomethoxy PEG as macro-initiator. The resulting copolymers were characterized by nuclear magnetic resonance, gel permeation chromatography, and critical micelle concentration analyses. Self-assembly of the copolymers yielded aggregates of different architectures, including spherical micelles and a mixture of spherical and worm-like micelles with Y-junctions. The self-assembled architecture depends on both the hydrophilic/hydrophobic balance and the molar mass of copolymers. Valsartan, a widely used drug in the treatment of hypertension, was loaded in micelles using a co-solvent evaporation method. High drug-loading content was obtained for worm-like micelles. in vitro drug release was performed at 37°C in pH 7.4 phosphate-buffered saline. An initial burst release is detected in all cases, followed by slower release up to 9 days. The overall release rate is strongly dependent on the degradation of micelles. Copolymers with short PLGA blocks exhibit faster drug release due to faster degradation of micelles, and worm-like micelles present slower drug release as compared to spherical ones. Therefore, PLGA-PEG copolymer micelles with high drug-loading capacity, different architectures, and variable drug release rates could be most attractive for sustained delivery of valsartan.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Yucheng Zou,Manjun Kang,Kaiyuan Cao,Xiaorui Su,Boyu Huaxing,Zeyu Qin,Abdullah,Jiyong Shi,Xiaowei Huang,Xiaobo Zou
Advanced delivery systems are crucial for overcoming the limitations of ferulic acid (FA), including its poor water solubility and stability. This study utilized γ-cyclodextrin metal-organic framework (γ-CD-MOF) to encapsulate FA, yielding FA-loaded γ-CD-MOF (FA@CD-MOF) nanocarriers, which were subsequently incorporated into zein/polyvinyl alcohol electrospun nanofibers to construct a dual-encapsulation system. Results showed that FA@CD-MOF exhibited an average particle size of 228.36 nm, and demonstrated efficient FA encapsulation with a loading capacity of 27.39% and encapsulation efficiency of 51.76%. The composite nanofibers exhibited enhanced thermal stability and moderate anti-swelling properties. In vitro release studies demonstrated that the γ-CD-MOF effectively suppressed initial burst release of FA, and led to a sustained release profile that conformed to the Fickian diffusion mechanism. Furthermore, the composite nanofibers exhibited long-lasting antioxidant activity owing to the protective effect (nano-in-nano) of the delivery system. Notably, in vitro digestion experiments showed that the bioavailability of FA in the nanofibers reached 74.66%, a 2.9-fold increase compared to free FA (26.11%). Cytotoxicity tests on Caco-2 cells supported the remarkable biocompatibility and biosafety of the developed delivery system. Collectively, this study presents a highly promising strategy utilizing γ-CD-MOF for the controlled delivery of FA, which significantly enhances its stability and bioavailability. Download: Download high-res image (405KB) Download: Download full-size image
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ACS Applied Bio Materials
·
2025
·
IF 4.7
Zhao Wang,Khawar Ali Shahzad,Ding Li,Boyu Cai,Zilin Huang,Maoxiang Xu,Jiaojiao Li,Dong Chen,Fei Tan
Monascin, a natural and safe drug, exhibits potent anti-inflammatory, hypolipidemic, and antioxidative effects. However, their therapeutic potential and underlying mechanisms in allergic rhinitis (AR) remain unexplored. The intranasal delivery of monascin for treating AR faces the challenge of rapid clearance. Herein, to achieve sustained and prolonged release, we developed monascin-encapsulated poly(lactic co glycolic acid) nanoparticles (PLGA-MS) by the mechanical double emulsion technique, enabling effective local delivery of monascin for the treatment of AR. Histopathological staining, flow cytometry, ELISA, and network pharmacology were used to assess therapeutic effects and potential mechanisms of monascin in treating AR. In vitro and in vivo experiments revealed that PLGA-MS exhibited enhanced stability, excellent drug encapsulation capacity, and sustained drug release. PLGA-MS treatment showed a significant therapeutic effect in AR mice by decreasing inflammatory cells in nasal tissue and regulating IgE, histamine, and a variety of cytokines. PLGA-MS treatment decreased T helper 2 cells (Th2) and T helper 17 cells (Th17) while promoting and increasing Tregs (regulatory T cells). Further analysis showed elevated levels of IL-10 and TGF-β expression in Tregs post-treatment. Results of the CCK-8 assay, pathological analysis of vital organs, and serum analysis of liver and kidney functions demonstrated the biosafety of PLGA-MS. Additionally, network pharmacology identified immune response pathways that may support PLGA-MS’s immunomodulatory effects in AR. Our study presented a biomaterial-facilitated intranasal delivery system for monascin, offering an effective therapeutic strategy for AR.
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