Frontiers in Pharmacology
·
2024
·
IF 4.4
Huiyuan Zhang,Quanbin Li,Yaxing Li,Jianhua Guan,Kaidi Li,Yunlong Chen
Background: Atopic dermatitis (AD) is among the most frequently encountered skin diseases, bothering a considerable number of patients. Today, corticosteroids and antihistamines are among the numerous drugs applied for the therapy of AD. However, lengthy use of them contributes to side effects, such as physiological changes in skin. As an alternative and supplementary therapy, traditional Chinese medicine has become a trend for AD treatment. Huang-Lian-Jie-Du decoction (HLJDD), a renowned herbal formula has been employed to treat inflammatory diseases such as AD. However, its role in regulating immunity in AD remains unclear. The object of this study was to elucidate the efficacy of HLJDD and reveal the implicit mechanism from an immunological perspective in AD-like mice.Methods: In brief, 1-chloro-2,4-dinitrobenzene (DNCB) for the sensitization phase (1% DNCB) and stimulation phase (1.5% DNCB) were applied for BALB/c mice. HLJDD and dexamethasone (DXMS) were administered orally to the mice. Mice skin and spleens were collected to evaluate the efficacy of HLJDD. 16S rRNA sequencing was applied to evaluate the commensal microbiota changes in skin and fecal. In vitro, spleen CD4+ T cells and bone marrow-derived mast cells (BMMCs) were co-cultured to explore the modulation of HLJDD in T helper (Th) cells phenotyping.Results: HLJDD showcased a substantial amelioration in skin through the upregulation of FLG, LOR, AQP3, and reducing scratching behaviors in AD-like mice, Also, the quantity of infiltrated mast cells (MCs), pruritus-related mRNA were decreased. In addition, the expression of OX40/OX40L was decreased by HLJDD, which was critical in Th-cell phenotyping. With the treatment of HLJDD, Th1/Th2 and Th17/Treg ratios in AD-like mice became balanced. The structure of commensal microbiota in AD-like mice was affected by HLJDD. HLJDD could also improve the imbalance of Th17/Treg in vitro.Conclusion: HLJDD could improve the symptoms of AD-like mice by alleviating the scratching behaviors via decreased Th2 and pruritus-related mRNA expression. HLJDD also enhanced the relative diversity of skin microbiota and changed the structure of intestinal microbiota. An in-depth study found that HLJDD could balance the ratio of Th1/Th2, Th17/Treg in AD-like mice, and Th17/Treg in vitro by regulating the OX40/OX40L signaling pathway.
研究领域:
免疫学
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皮肤病学
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中医
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微生物学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-9
·
IF 5.479
Chen Dan,Xu Hui
The exploration of monohydroxy polycyclic aromatic hydrocarbons and 8-hydroxy-2′-deoxyguanosine (8-OHdG) produced by oxidative stress and DNA damage is a powerful and non-invasive tool to study the health risk of exposure to polycyclic aromatic hydrocarbons (PAHs). A nanocomposite prepared from graphene oxide, poly(3,4-ethylenedioxythiophene) and polypyrrole was electrodeposited on the internal surface of a stainless-steel tube for online in-tube solid phase microextraction (IT-SPME) of 8-OHdG, 3-hydroxyphenanthrene and 1-hydroxypyrene from urine. The coating possesses excellent chemical and mechanical stability, high extraction efficiency, good resistance to matrix interference, and a long lifespan. An online IT-SPME-high performance liquid chromatography-mass spectrometry method was developed for the determination of these three metabolite biomarkers in human urine. Figures of merit include (a) enrichment factors of 30–48; (b) low limits of detection (4–41 pg·mL −1 at S/ N = 3); (c) wide linear ranges (0.05–50 ng·mL −1 ); (d) good recoveries from spiked samples (71.6–109.5%); and (e) acceptable repeatability (2.3–14.6%). The method offers the advantages of low cost, simplicity, sensitivity, rapidity and automation.
Schematic illustration of online in-tube solid phase microextraction using graphene oxide/poly(3,4-ethylenedioxythiophene)/polypyrrole composites as adsorbent in a stainless-steel (SS) tube for the enrichment and simultaneous determination of 8-hydroxy-2′-deoxyguanosine, 3-hydroxyphenanthrene and 1-hydroxypyrene prior to HPLC-MS analysis.
研究领域:
分析化学
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环境健康
·
毒理学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Yong Xu,Yue Wang,Ping Wang,Yishun Wang,Weili Dai,Jianping Zou,Xubiao Luo
Employing electric energy to convert carbon dioxide (CO 2 ) into valuable small molecules is a potentially practical method in energy storage and greenhouse gas alleviation. A huge challenge for electrocatalytic CO 2 reduction is to reduce overpotential to improve energy efficiency. Herein, we demonstrate that doping alloy Pd 49 Ag 30 Te 4 (PAT) with rare-earth element Tb is beneficial for selective exposure of (1 1 1) crystal plane, which is a highly active crystal plane for producing carbon monoxide (CO). The as-prepared Tb 2.9 PAT exhibited high electrocatalytic performance with 95.7 % CO faradic efficiency at − 0.8 V (vs RHE), far exceeding that of PAT, and coupled with good durability. In situ spectral study and theoretical calculations disclose that the introduction of Tb regulates the d-band center of PAT alloy, weakens the Pd − C bonding ability, and promotes the desorption of *CO in the rate-determining step. This study provides a method for doping induced selective exposure of crystal face, which provides new idea for improving catalytic performance.
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PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2022
·Vol. 19
·期 6
·e202200286
·
IF 2.745
Shu-Hua Zhang,Xian-Lin Yue,Xue Zhao,Jing Tang,Yu Yang,Rui Xu,Hao Ma,Shuai-Ming Zhu,Fu-Yao Luo,Qin Zhang,Guo-Gang Zhang,Chang-Wei Li
Longibrachiamide A ( 1 ), a new 20-residue peptaibol, along with three known ones ( 2 – 4 ), were isolated from the fungus Trichoderma longibrachiatum Rifai DMG-3-1-1, isolated from a mushroom Clitocybe nebularis (Batsch) P. Kumm, which was collected from coniferous forest of northeast China in our previous work. The structure of longibrachiamide A ( 1 ) was determined by its NMR and ESI-MS/MS data, the absolute configuration of 1 was further determined by Marfey's analyses. And the complete NMR data of 2 – 4 were also reported for the first time. The similar CD spectra of 1 – 4 showed that they all had mixed 3 10 -/α-helical conformations. Compounds 1 – 4 showed strong cytotoxicities against BV2, A549 and MCF-7 cells, and also showed moderate inhibitory effects against the tested Gram-positive bacteria, including MRSA T144 and VRE-10. Graphical
研究领域:
微生物学
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药物化学
·
生物化学
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DOI
高影响因子
OncoTargets and Therapy
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2023
·
IF 4
Chao Wu,Meng-hua Zeng,Gang Liao,Kun Qian,Hui Li
Introduction Neuropilin-1 (NRP1) binds to many ligands and co-receptors and affects cell survival and migration, which is essential for tumor progression. However, there are still largely unknowns about how NRP1 affects the epithelial–mesenchymal transition (EMT)-related malignant progression in gastric cancer.Methods We used TCGA to analyze the expression of NRP1 in gastric cancer and its impact on patient survival. In in vitro experiments, transwell, wound healing and colony formation assays were used to evaluate the effects of NRP1 and ginsenoside Rg3 on the invasion, migration and proliferation of gastric cancer cells. In in vivo experiments, we evaluated the overexpression and knockdown of NRP1 and the effect of ginsenoside Rg3 on tumor growth.Results We found that NRP1 is highly expressed in advanced gastric cancer and associated with poor prognosis. Knockdown of NRP1 expression can inhibit the proliferation and metastasis of gastric cancer cells. Mechanically. NRP1 interacts with fibronectin-1 (FN1) to promote the malignant progression of gastric cancer cells through ECM remodeling. In addition, we found that ginsenoside Rg3 can block the interaction of NRP1 and FN1 and inhibit the progression of gastric cancer.Conclusion Our study suggested that the interaction of NRP1 and FN1 is crucial for the malignant progression of gastric cancer. This may provide a new perspective and potential treatment methods for the treatment of gastric cancer.
研究领域:
肿瘤学
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细胞生物学
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分子生物学
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癌症生物学
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DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2020
·Vol. 5
·期 3
·1477-1485
·
IF 6.788
Jing Wang,Shuping Xu,Aisen Li,Lei Chen,Weiqing Xu,Hongyu Zhang
A polymorph is an ideal crystal model for studying the structures affect the optical waveguide behaviors of organic cocrystals, resulting from their multiple crystal structures with the same compositions. However, the polymorphism-based optical waveguide properties of cocrystals are rarely reported. Here, two polymorphic cocrystals of 9-acetylanthracene-1,2,4,5-tetracyanobenzene in a novel sheet-like form α and a known bulk form β were prepared by the evaporation of a donor (D)/acceptor (A) solution mixed in a ratio of 1 : 1 in a mixed acetonitrile and tetrahydrofuran solution. The two polymorphs present different molecular stacking structures due to the diverse intermolecular hydrogen bond interactions, causing differences in their intermolecular charge transfer (CT) interactions and photophysical properties. Structural and spectroscopic analyses revealed stronger CT transition in the form β than in the form α. Polymorphism-based luminescence properties and morphology- and color-dependent optical waveguide behaviors were observed in the polymorphic CT cocrystals via photoluminescence (PL) micro-imaging. The propagation losses of the form α were 0.37 and 0.6 times less than those of the form β in parallel and perpendicular direction with respect to the growth direction, respectively. Three-dimensional (3D) stereoscopic mode optical waveguide behaviors can be observed in the form β. The study provides a good method for the preparation and detection of polymorphic materials. The optical waveguide properties of the cocrystals were tuned by the crystal phase, which is helpful for a deep understanding of the structure-optical functional relation in organic cocrystals. The study will open new potential applications of cocrystal materials in 3D photonic and optoelectronic devices.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2014
·
IF 2.045
Guo-Min Yu,Li-Sheng Wang,Chao-Jun Du
A phosphorus flame retardant of 4-(hydroxymethyl)-1-oxido-2,6,7-trioxa-1-phosphabicyclo[2.2.2]octane (PEPA) was synthesized by pentaerythritol and phosphorus oxychloride at a certain condition. The structural features of PEPA were investigated with Fourier transform infrared spectroscopy and 1H NMR methods. The thermal behavior of PEPA was carried out with differential scanning calorimetry and thermogravimetric analysis instruments. A static analytic method was used to measure the solubilities of the PEPA in several organic solvents. The measurements were accurately acted in ethyl acetate, acetonitrile, isopropyl alcohol, ethanol, n-propyl alcohol, acetone, methanol, and chloroform at the temperature range from 292.75 K to 333.45 K. Using the Scatchard–Hildebrand methodology, the activity coefficients of the solute have been calculated, and the partial molar excess enthalpies at infinite dilution were also predicted.
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DOI
高影响因子
EMBO JOURNAL
·
2024
·
IF 9.4
GengShan,YangShan,TangXuejiao,XueShiyao,LiKe,LiuDongfang,ChenChen,ZhuZhiming,ZhengHongting,WangYuanqiang,YangGangyi,LiLing,YangMengliu
Communication of gut hormones with the central nervous system is important to regulate systemic glucose homeostasis, but the precise underlying mechanism involved remain little understood. Nesfatin-1, encoded by nucleobindin-2 (NUCB2), a potent anorexigenic peptide hormone, was found to be released from the gastrointestinal tract, but its specific function in this context remains unclear. Herein, we found that gut nesfatin-1 can sense nutrients such as glucose and lipids and subsequently decreases hepatic glucose production. Nesfatin-1 infusion in the small intestine of NUCB2-knockout rats reduced hepatic glucose production via a gut – brain – liver circuit. Mechanistically, NUCB2/nesfatin-1 interacted directly with melanocortin 4 receptor (MC4R) through its H-F-R domain and increased cyclic adenosine monophosphate (cAMP) levels and glucagon-like peptide 1 (GLP-1) secretion in the intestinal epithelium, thus inhibiting hepatic glucose production. The intestinal nesfatin-1 -MC4R-cAMP-GLP-1 pathway and systemic gut-brain communication are required for nesfatin-1 - mediated regulation of liver energy metabolism. These findings reveal a novel mechanism of hepatic glucose production control by gut hormones through the central nervous system.
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Shuangqi Song,Hong Li,Jingde Li,Yanqin Yang
Mixed-matrix membranes (MMMs), developed through the combination of solid fillers with polymer matrices, represent an effective approach addressing the persistent challenge of balancing permeability and selectivity in polymer membrane. Diethylenetriamine-functionalized porous material of PP-DETA, which is synthesized from porous organic polymer of PP via one-step diethylenetriamine grafting, is an ideal material for MMMs production. However, designing and optimizing PP-DETA for high-performance CO 2 separation membranes remains a challenge. Herein, we develop an improved PP-DETA material, designated as PP-DETA-cPIM-1, by covalently attaching a carboxylated polymer of intrinsic microporosity (cPIM-1) network layer onto the PP-DETA surface. Membranes prepared from this PP-DETA-cPIM-1 filler and PIM-1 matrix demonstrate enhanced interfacial adhesion and stronger mechanical properties in comparison to the counterparts with the same loading of PP-DETA. The incorporation of PP-DETA-cPIM-1 into MMMs establishes an enhanced filler/polymer interface that facilitates the selective transport of CO 2 through the filler, while simultaneously improving membrane stability and suppressing physical aging. Specifically, with 5 wt% loading of PP-DETA-cPIM-1 filler, the MMM is able to enhance the CO 2 /N 2 selectivity and CO 2 permeability by 52.3 % and 25.3 %, respectively, exceeding the upper bound established in 2008. Furthermore, PP-DETA-cPIM-1/PIM-1 MMMs display superior anti-aging ability for 180 days of aging.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Rui Huang,Lizhi Lin,Jinghua Zhang,Shaohua Jin,Qingquan Tian,Ziming Yang,Puwang Li,Yu Chen
Dihydroxylammonium 5, 5′-bistetrazole-1, 1′-diolate (TKX-50) is a new high-energy and insensitive material with broad application prospects. However, the discharge of TKX-50 wastewater from its production and applications not only causes the waste of high-value explosives, but also pollutes the environment. The traditional used adsorbents are poor adsorption efficiencies and recyclability performance in pratical applications. In this study, we successfully prepared a porous polyurethane/chitosan hybrid foam (PUF) with chitosan (CTS) as the active adsorption component by the semi-prepolymerization/acidification method. The compressive strength of the PUF can reach up to 8.83 kPa, and the static adsorption capacity of TKX-50 can reach 39.32 mg/g. The TKX-50 adsorption process and mechanism were further studied by the adsorption kinetics, isotherm and thermodynamics. The adsorbed TKX-50 was effectively recovered by NaOH solution, with a desorption rate exceeding 94 %. The dynamic adsorption investigation indicates that the PUF is suitable for industrial continuous flow wastewater treatment process in practical engineering. In all, our work has explored a new construction strategy of chitosan composited recyclable polyurethane hybrid foam materials with high TKX-50 adsorption efficiencies, and the obtained hybrid foam exhibits great application potentials for the treatment of TKX-50-containing wastewaters.
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Fangqi Cao,Shuchen Yu,Xiujuan Chen,Lu Xiao,Tingting Qiu,Xiru Wang,Daiwen Zhang,Xiaoliang Yuan,Ping Shi
Synthetic cannabinoids (SCs) are an evolving class of new psychoactive substances (NPS) with structurally various compounds that are increasing over the past few years. Therefore, they are initially hard to identify because of the lack of analytical information. Moreover, there is little to no information regarding the pharmacology of these compounds despite human abuse. In the present study, gas chromatography-mass spectrometry (GC-MS), ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS), and nuclear magnetic resonance (NMR) spectroscopy were used to identify the structure of three compounds obtained from seized materials. The pharmacological properties of these compounds were evaluated by subsequent behavioral testing, including von Frey and cold allodynia tests. The results indicated that these compounds were determined to be 2-(1 H -indazole-3-carboxamido)-3,3-dimethylbutanoate (MDMB-INACA), N-(1-amino-3,3-dimethyl-1-oxobutan-2-yl)-1 H -indazole-3-carboxamide (ADB-INACA), and N-(1-amino-3,3-dimethyl-1-oxobutan-2-yl)-1-hexyl-1 H -indazole-3-carboxamide (ADB-HINACA) via GC-MS, UPLC-Q-TOF MS and NMR analysis, and they can attenuate mechanical and cold allodynia induced by paclitaxel in rats with peripheral neuropathy. Compared with MDMB-INACA and ADB-HINACA, ADB-INACA showed better analgesic effects on paclitaxel-induced peripheral neuropathy (PIPN) in rats, and its effect was similar to that of the positive drug N′-(1-hexyl-2-oxoindolin-3-ylidene) benzohydrazide (MDA-19).
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DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
·
2021
·
IF 2.269
Peng Zhou
A new material, polyacrylic acid-b-polyptyrene (PAA-b-PS) covered Ni/Fe nanoparticles (PAA-b-PS-nZVI-Ni), was developed and evaluated for the selective dechlorination of 1,1,1-trichloroethane (1,1,1-TCA) in the presence of potential interferents. The average size of the PAA-b-PS coated Fe/Ni bimetallic nanoparticles was approximately 50 nm, which resulted from agglomeration prevention by PAA-b-PS. The removal efficiency of 1,1,1-TCA by an optimal dose with a 1.0 g/L Fe/Ni (Ni/Fe = 2 wt%) and 0.5 g/L PAA-b-PS-coated concentration was higher (87.5%) than that of bare Fe/Ni (60%). The pseudo-first-order rate constant (Kobs) of 1,1,1-TCA removal by PAA-b-PS-nZVI-Ni was 0.0142 min−1 within 240 min. Comparatively, the Kobs values of 1,1,1-TCA removal by other materials (Fe, pure bimetallic Fe/Ni, PAA-b-PS-Fe) were only 0.003, 0.0052 and 0.0103 min−1, respectively. The 1,1,1-TCA removal efficacy by PAA-b-PS-nZVI-Ni showed no obvious gap regardless of pH or various common inorganic anions (NO3−, HCO3− and SO42−) at different concentrations. However, humic acid (HA) had great influence on the degradation of 1,1,1-TCA. In conclusion, PAA-b-PS-covered Ni/Fe nanoparticles with its selectivity high effectiveness could be used as one remedial agent for the degradation of 1,1,1-TCA in water.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
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