JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2020
·
IF 3.004
Xiangrong Wu,Beibei Qiu,Yanan Chen,Yuqiong Shi,Jinhua Zhu,Xiuhua Liu,Dongbao Zhao
In this work, α-glucosidase was immobilized on a type of nanoscaled magnetic materials Fe 3 O 4 @ZIF-67 to construct a biomicroreactor for rapid screening α-glucosidase inhibitors. The parameters that affected the immobilization efficiency were investigated. Under optimal conditions, the amount of α-glucosidase loaded was up to 79.07 μg·mg −1 . Then, the enzyme biomicroreactor was immobilized by an external magnetic field in a tube connecting HPLC and micro-injection pump at both ends to form a magnetic online screening system. The screening flow rate and eluent organic solvent ratio during the screening process were optimized. XinYang MaoJian crude tea extract was tested to verify this online screening method, and three inhibitors (catechin, epigallocatechin gallate and epicatechin gallate) were fished by this magnetic online screening system. Based on the classical p NPG method, the inhibitory activities on α-glucosidase were further verified and studied. Compared with the traditional ligand fishing methods, the screening method established in this work can integrate screening, elution and analysis. It can simply, efficiently and directly screen and identify potential α-glucosidase inhibitors from natural sources. This method was expected to provide an effective basis for accelerating the development of new hypoglycemic drugs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2019
·
IF 6.944
Liping Liu,Zhen Fang,Xiangjiang Zheng,Dongmei Xi
A straightforward yet efficient, nanopore-based strategy that enables the sensitive detection of copper(II) ion (Cu2+) and real-time monitoring of a click reaction is provided. Two single-stranded DNAs (ssDNAs) are designed to act as the preprobes, one being modified with an azide and the other an alkyne. The presence of Cu2+ induces the ligation of two ssDNAs via click reaction, leading to the formation of a forked DNA which can quantitatively generate characteristic current signatures when interacts with α-hemolysin (α-HL) nanopore. The assay facilitates a highly selective and sensitive measurement of Cu2+ without the need for labels or signal amplification. More importantly, this nanopore platform exhibits excellent performance in real-time monitoring of a copper(I) ion (Cu+)-catalyzed click reaction at the single-molecule level, by recording the current signals of the forked DNA generated by click chemistry. The proposed strategy is believed to play an important role in both nanopore sensing and characterization of chemistry reactions, especially coupling reactions.
研究领域:
纳米孔传感
·
化学反应表征
·
生物分析化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2025
·
IF 4.3
Yawei Zhao,Yuqing Liu,Tailai Yan,Malcolm Xing,Wen Zhong
Hydrogels have acquired numerous attentions in biomedicine and smart bioelectronics due to similar properties to our tissues. Initiators are crucial in vinyl polymerization for hydrogel preparation which can influence mechanical properties and physical/chemical structures. We report α-ketoglutaric acid (αKG), an intermediate in the citric acid cycle, as an ecofriendly photoinitiator for vinyl polymerization. We comprehensively investigated the mechanical properties of hydrogels initiated by comparing αKG with Irgacure 2959 (I-2959) and ammonium persulfate (APS). We found that αKG successfully initiated the polymerization in both synthetic and natural hydrogels (polyethylene glycol, polyacrylamide, gelatin, alginate, and chitosan). Meanwhile, the interactions between αKG and functional groups of macromolecules make αKG a potential additive in hydrogels, which could influence the hydrogel mechanics. In contrast to the potential toxicity of unreacted APS and the limited water solubility of I-2959, αKG proves to be a more versatile and sustainable option in gelation than the other two. This work throws new light on hydrogel fabrication with the initiator alteration for potential biomedical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2025
·
IF 3.9
Haichun Zeng,Jiatong Zeng,Beilin Meng,Yuxin Zhou,Kai Zhou,Li Rao
Sesquiterpenes are a diverse class of natural products characterized by complex structures and broad biological activities with their core scaffolds formed by sesquiterpene synthase. In this study, a novel sesquiterpene synthase, NmSTS, from the fungus Neophaeococcomyces mojaviensis was functionally characterized. NmSTS catalyzes the cyclization of (E,E)-farnesyl pyrophosphate ((E,E)-FPP) to produce the bridged ring sesquiterpene α-santalene, a compound widely used in the fragrance and flavor industries. Additionally, NmSTS also exhibits substrate promiscuity, converting (E)-geranyl pyrophosphate ((E)-GPP) and (E,E,E)-geranylgeranyl pyrophosphate ((E,E,E)-GGPP) into linear terpenes. Through protein modeling and site-directed mutagenesis, ten key amino acid residues essential for catalytic activity were identified. Among these, the R187A and Y302A variants enable the transformation of the sesquiterpene product from a bridged ring to hydroxylated tri- and acyclic skeletons, respectively. These findings enhance our understanding of sesquiterpene biosynthetic mechanisms and provide valuable genetic tools for the engineered microbial production of valuable sesquiterpenes.
研究领域:
微生物学
·
生物化学
·
分子生物学
·
合成生物学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2013
·Vol. 40
·期 1
·2395-2399
·
IF 1.789
Changbai Liu,Hao Shan,Li Liu,Shouchun Li,Haiying Li
Pristine and Ce-doped (3, 4, 5 at% Ce) α -Fe 2 O 3 nanotubes were synthesized by electrospinning method . The morphologies and structures of these samples were characterized by scanning electron microscope (SEM) and X-ray powder diffraction (XRD). The gas sensing properties of the four samples were investigated. The results showed that the sensitivity of the 4 at% Ce-doped α -Fe 2 O 3 nanotubes reached 21.5–50 ppm acetone at 240 °C, which was about 8.3 times larger than that of pure α -Fe 2 O 3 nanotubes . Meantime, the response and recovery times were about 3 and 8 s, respectively. The sensitivity of the lowest detecting limit to 1 ppm acetone was about 3. Moreover, the sensor also possessed the good selectivity and long-term stability to acetone.
研究领域:
材料科学
·
纳米技术
·
气体传感
·
化学传感器
✓ 已验证
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 114
·93969-93978
·
IF 3.84
Yingjie Zhou,Aoning Wang,Zhoulu Wang,Miao Chen,Wei Wang,Luyi Sun,Xiang Liu
Titanium species were immobilized on α-ZrP nanosheets (ZrP–Ti) using a modified post-grafting method. The obtained ZrP–Ti composites were characterized via Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), ultraviolet-visible (UV-vis) absorption spectroscopy, photoluminescence spectroscopy (PL), and scanning electron microscopy (SEM). Titanium species in the form of TiO2−x clusters with an average particle size of 2–5 nm were grafted on α-ZrP nanosheets via chemical bonding with P element. These ZrP–Ti composites could be well dispersed in polar solvents to ensure that the TiO2−x clusters bonded on both faces of the nanosheets were accessible. The ZrP–Ti composites presented enhanced visible-light absorption properties compared with that of pure TiO2. These features allowed them to exhibit better photocatalytic activities for the photodegradation of methylene blue (MB) under solar irradiation. The TiO2−x clusters tended to aggregate into anatase phase TiO2 when the molar ratio of P/Ti exceeded 1 : 1, and this reversed the advantage in their photocatalytic activities. The best MB degradation efficiency of 100% with the apparent rate constant of 0.054 min−1 was achieved over the ZrP–Ti composite with the P/Ti molar ratio of 1 : 1.
✓ 已验证
DOI
高影响因子
CATALYSIS LETTERS
·
2019
·Vol. 149
·期 7
·2032-2042
·
IF 2.372
Wang Ying,Shen Shuguang,Li Meina,Sun Yujuan,Li Binbin,Ma Xueping,Zhou Zijian,Guo Chenyuan
Abstract MnO 2 was screened from various metal oxides and different crystal structures of MnO 2 were prepared, which were used to hydrolyze urea process wastewater for energy conservation and environmental protection. The characterizations of XRD, SEM, Raman, FT-IR, XPS and H 2 -TPR were employed and the hydrolysis experiments were carried out, demonstrating that oxygen vacancy on the surface of MnO 2 might be the catalytic activity source on urea hydrolysis. By optimizing reaction influencing factors, the residual urea concentration reduced to 1.61 mg/L over α -MnO 2 , far below the effluent standard. Meanwhile, the temperature of heterogeneous hydrolysis over α-MnO 2 dropped drastically compared with that of thermal hydrolysis. Further, the kinetic parameters of hydrolysis were obtained over α -MnO 2 . And α -MnO 2 also showed excellent recycling ability on the reused test. Graphical Abstract
Effect of different crystal structures MnO2 on urea degradation rate. (Catalyst dosage is 50 g/L, reaction time is 60 min, temperature is 165oC.)
研究领域:
环境化学
·
催化科学
·
废水处理
·
材料化学
·
绿色能源
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Jing Zhao,Yangning Zhang,Tuo Wang,Peng Zhang
The electrochemical CO2 reduction reaction (CO2RR) provides a renewable avenue to convert CO2 to value-added fuels and chemicals. Bismuth (Bi)-based electrocatalysts are known as promising candidates for CO2RR to formate (HCOO–), yet their performance is frequently limited by structural reconstruction during operation, resulting in reduced activity and selectivity. This work describes a thermal treatment approach to tuning the catalytic chemistry of α-Bi2O3 for CO2RR. A thermally optimized Bi-400 electrocatalyst achieves a high Faradaic efficiency of 93.0% for HCOO– at −0.95 V vs RHE, along with good stability over 70 h. Through detailed structural and mechanistic studies, it is revealed that the preserved Bi–O motifs in Bi-400 facilitate CO2 activation and modulate the binding energy of the *OCHO intermediate, thereby enhancing both activity and selectivity toward HCOO– production. This study would provide an in-depth understanding of the selective formation of HCOO– products on Bi-based electrocatalysts in CO2RR.
✓ 已验证
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Sijia Zheng,Huige Ren,Haiyan Xue,Lingqi Huang,Jianfeng Zhang,Zhihai Cao
With the emergence of soft electronics, composite materials with a combination of robust adhesion, reprocessing and recycling capability, electrical and thermal conductivity, and biocompatibility are highly required. α-Lipoic acid–based silicone composites are fabricated by a simultaneous ring-opening reaction of epoxide-functionalized silicone with α-lipoic acid and ROP of α-lipoic acid. The liquid metal (LM) is incorporated via −COOH-LM- and -S-LM- coordination. The resultant LM-LAPs exhibited robust adhesion to various substrates. The LM-LAPs exhibited efficient reprocessability and recycling capability enabled by carboxylic groups, disulfide exchange, and depolymerization of the disulfide backbone. Thermal and electrical conductivity reach 2.35 W m–1 K–1 and 19.48 S m–1 for LM-LAP70. Notably, the optimized LM-LAP50 was successfully used in monitoring body movement. This design offers a new route for creating silicone elastomers with technological implications for flexible electronics.
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Mengqi Teng,Yuqing Zhang,Guorui Yang,Jiayu Sun,Haifang Xiao
This study aimed to characterize, clarify the α-glucosidase inhibitory activity and mechanism of phlorizin-Zn (II) complex (Phl-Zn) and phlorizin-Cu (II) complex (Phl-Cu). Phl-Zn and Phl-Cu were synthesized and preliminarily characterized using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), elemental analysis (EA) and thermogravimetric analysis (TGA). The formation of stable coordination structures between phlorizin and metal ions (Cu 2+ /Zn 2+ ) through metal-oxygen bonds was confirmed. Furthermore, inhibition kinetics and multispectral techniques including ultraviolet spectrophotometry and fluorescence spectrophotometry were employed to investigate the inhibitory effects of phlorizin and its complexes on α-glucosidase. Phlorizin, Phl-Zn and Phl-Cu reversibly inhibited the enzyme activity with IC50 values of 0.766 ± 0.012, 0.470 ± 0.035 and 0.084 ± 0.019 mg/mL, respectively, demonstrating superior efficacy of the metal complexes. Both complexes exhibited mixed-type inhibition and stronger binding affinity to α-glucosidase than the enzyme-substrate interaction (K is > K i ). Furthermore, the binding constants (Ka) of phlorizin and its two metal complexes toward the enzyme decreased with rising temperature, suggesting that their stability was weakened at higher temperatures. Additionally, both complexes bound to the single active site of α-glucosidase via hydrogen bonds and van der Waals forces, which induced fluorescence quenching, increased tryptophan polarity and reduced hydrophobicity, and finally altered the enzyme's conformation and secondary structure. It was concluded from molecular docking and molecular dynamics analysis that Phl-Cu exerted more significant stabilizing effect on the protein structure than phlorizin and exhibited favorable dynamic adaptability in its binding mode. This study provides supporting information to develop novel phlorizin-based α-glucosidase inhibitors.
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2026
·
IF 6.6
Zhenyi Zhu,Dan Li,Jirui Lin,Bowen Lv,Litian Wang,Cuiping Li
Facile strategies for regulating the microstructure and surface chemistry of metal oxide semiconductors are essential for advancing gas sensing technologies. Metal–organic frameworks (MOFs) with well-defined structures serve as ideal sacrificial templates for designing high-performance metal oxide gas sensors. In this work, a series of α-Fe 2 O 3 materials with tunable nanostructures were successfully synthesized via the calcination of NH 2 -MIL-88B(Fe) precursors, where the FeCl 3 /Fe(NO 3 ) 3 molar ratio was systematically varied. The influence of the dual‑iron‑source composition on the morphology, structure, and gas‑sensing properties of the resulting α-Fe 2 O 3 samples (denoted as CN x , x = 1–5) was systematically investigated. The results demonstrate that the CN3 sensor exhibits a significantly higher response (27.9) to 100 ppm acetone at 260 °C compared to the other samples. Moreover, the CN3 sensor shows a low detection limit, excellent selectivity, satisfactory repeatability, and long-term stability. The enhanced acetone-sensing performance is mainly associated with the synergistic effects of a well-interconnected three-dimensional porous network and abundant oxygen vacancies, which together facilitate gas diffusion, promote oxygen adsorption and surface reactions, and improve the effective utilization of active sites. This study provides a feasible precursor-engineering strategy for fabricating advanced α-Fe 2 O 3 ‑based gas sensors and highlights their potential for non-invasive diabetes monitoring through breath acetone detection.
✓ 已验证
DOI
高影响因子
Cell Systems
·
2026
·
IF 7.5
Huixia Ren,Yanjun Li,Beichen Xie,Zhenchao Fu,Xiaohong Peng,Weiran Qian,Yi Yu,Tianyi Chang,Xiaojing Yang,Kim Sneppen,Liangyi Chen,Chao Tang
Glucose-stimulated hormone secretion in pancreatic islets is closely linked to oscillations in cytoplasmic Ca 2+ , which arise from complex intra- and intercellular signaling. δ cells, intermingled with peripheral α cells, are important paracrine regulators, but their role in shaping Ca 2+ oscillations remains unclear. Here, we show that δ-α cell interactions contribute to the variability of glucose-induced Ca 2+ oscillation patterns. Somatostatin released by δ cells prolonged the oscillation period in an α cell-mass-dependent manner. Pharmacological and optogenetic perturbations of δ-α interactions prompted an oscillation transition. Continuous adjustment of δ-α coupling strength caused the fast-oscillating islets to transition to mixed and slow oscillations. Mathematical modeling indicated that this fast-mixed-slow transition is a Hopf bifurcation. In vivo , blood glucose correlated with oscillation mode: hyperglycemia with slow oscillations and euglycemia with fast oscillations. These findings explain how δ and α cells shape islet Ca 2+ dynamics, a phenomenon dictated by the diverse cytoarchitecture of the islet. A record of this paper’s transparent peer review process is included in the supplemental information .
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）