CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Xin Rao,Yujing Niu,Qiantao Zhang,Xingcan Chen,Fangmei Zhou,Mingyuan Zhou,Yuchi Chen,Yingzhi Shen,Jie Yu,Bingqi Zhu,Zhishan Ding
Diabetic foot ulcers pose a significant clinical challenge due to dysregulated immune responses, neuropathic complications, and persistent infections that synergistically disrupt physiological tissue regeneration mechanisms. While immune activation is essential for orchestrating wound repair, excessive inflammation often exacerbates tissue damage in chronic diabetic wounds. To address these multifactorial issues, we designed a dual-crosslinked hydrogel platform integrating alginate, Bletilla striata polysaccharides (BSP), and gelatin to encapsulate tannic acid/Fe 3+ (TA/Fe 3+ ) composite nanoparticles with synergistic photothermal antibacterial treatment function. In vitro studies demonstrated that the dual-crosslinked hydrogel modulated immune microenvironment through effective scavenging of pathological reactive oxygen species (ROS) and significant downregulation of pro-inflammatory cytokine expression and promoting fibroblast migration. In addition, TA/Fe 3+ endowed the hydrogel with antibacterial activity under near-infrared (NIR) irradiation. The macrophage phenotype study revealed that the hydrogel promoted anti-inflammatory phenotype reprogramming of bone marrow-derived macrophages (BMDMs) under lipopolysaccharide (LPS) stimulation, which may be achieved by down-regulating the nuclear expression of pyruvate kinase M2 (PKM2). In the in vivo evaluation of the diabetic mouse model, the ability of the hydrogel to accelerate wound closure was verified, and the neovascularization at the lesion site was strongly stimulated. Through the temporal and spatial control of the healing process, the hydrogel has become an integrated treatment platform for chronic infection wound management, showing potential clinical application value.
研究领域:
生物材料
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免疫治疗
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伤口愈合
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纳米医学
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组织工程
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生物医学工程
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Te Yu,Yonggang Dai,Zifei Wang,Yang Gao,Qiong Wu
High molecular mass and poor solubility of Auricularia auricula polysaccharides (AAP) limit their physiological activity. This study employed ultrasonic degradation to address these challenges. Ultrasonic treatment reduced the molecular mass of AAP from 1 × 10 5 Da to 3.8 × 10 3 –8 × 10 4 Da, enhanced hydroxyl group exposure, and significantly improved solubility, with dissolution time reduced to one-ninth of the pre-degradation level at 20 °C. The antioxidant activities of AAP were enhanced after ultrasonic degradation, with DPPH scavenging IC 50 reduced from 3.58 mg/mL to 2.07 mg/mL and hydroxyl radical IC 50 from 2.28 mg/mL to 1.77 mg/mL. Hypoglycemic effects were improved, with α-amylase and α-glucosidase inhibition rates increasing by 5.06 % and 5.81 %, respectively. Bile acid binding for MBS improved by 28.6 %, indicating ultrasonic degradation enhanced hypolipidemic activity. This study demonstrates ultrasonic degradation is a green, efficient method to improve AAP's functional properties, which broadens its applications in functional foods and health supplements.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
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2024
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IF 3.2
Hongkun Xue,Lamu Nima,Shuhe Wang,Jiaqi Tan
Ultrasound assisted hot water extraction (UAHWE) was applied to extraction of polysaccharides from Taraxacum mongolicum with hot water as extract solvent. Experimental factors in UAHWE process were optimized by response surface methodology. The optimal extraction parameters to achieve the highest Taraxacum mongolicum polysaccharides (TMPs) yield (12.08 ± 0.14)% by UAHWE were obtained under the ultrasound power of 200 W, extraction temperature of 62°C, solid-to-liquid ratio of 1:20 g/mL, and extraction time of 40 min, and then the crude TMPs were further purified by DEAE-52 and Sephadex G-100 chromatography to obtain a homogenous polysaccharide fraction (TMPs-1-SG). Subsequently, the structure of TMPs-1-SG was characterized by UV-vis, Fourier transform infrared spectroscopy (FT–IR), high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), scanning electron microscope (SEM), transmission electron microscopy (TEM), and Congo red test. The results display that TMPs-1-SG with an average molecular weight of 5.49 × 10 4 Da was comprised of mannose (Man), galactose (Gal), xylose (Xyl), and arabinose (Ara) with a molar ratio of 39.85:52.61:27.14:6.30. Moreover, TMPs-1-SG did not contain a triple helix structure. Furthermore, TMPs-1-SG and TEM presented a sheet-like, rod-shaped, and irregular structure. Finally, the antioxidant activity of TMPs-1-SG was evaluated by in vitro experiment. The IC 50 values of scavenging DPPH and OH radicals for TMPs-1-SG achieved 0.71 mg/mL and 0.75 mg/mL, respectively. The findings can provide an effective method for extracting polysaccharides from natural resources.
研究领域:
植物化学
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食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
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2024
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IF 2.6
Yanhua Tian,Aiguo Luo,Zhaoyan Yang,Ling Zhang,Xuanping Zhang
Ultrasound assisted aqueous two-phase extraction (UATPE) is used to extract polysaccharides from Ophiopogon japonicus (OJPs). Experimental factors in UATPE process are optimized by response surface methodology. The optimal extraction conditions are obtained as follows: the ethanol concentration of 34%, ammonium sulfate concentration of 23%, solid-to-liquid ratio of 1:17 g/mL, and ultrasound power of 480 W, and the yield of OJPs is 9.41% ± 0.12%, and then the crude OJPs are further purified by different column chromatography to obtain a homogenous fraction (OJPs-2-SG), and identified its structure via different methods. OJPs-2-SG with a molecular weight of 1.25 × 105 Da is comprised of mannose (Man), galactose (Gal), glucose (Glc), and rhamnose (Rha) with a molar ratio of 5.15: 26.39: 68.72:8.41. Moreover, OJPs-2-SG contained α and β glycosidic bonds, and didn't showed a triple helix structure. Furthermore, OJPs-2-SG described irregular structures, and cylindrical and conical block like features. The IC50 values of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH), and superoxide (O2-) radicals for OJPs-2-SG achieved 3.72 mg/mL, 3.89 mg/mL, and 8.71 mg/mL, respectively. These results provide important references for the extraction and application of polysaccharides.
研究领域:
植物化学
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食品科学
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生物化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Shuqin Wang,Kai Wang,Wangbei Cao,Liang Song,Shifen Li,Zihe Zhai,Liyin Shen,Yang Zhu,Wenxing Liu,Changyou Gao
The ingrowth of endogenous cells into tissue scaffolds and modulation of Treg population take a pivotal function in restoring the structure and function of heart post-acute myocardial infarction (MI). However, the traditional hydrogels are failed to achieve such functions due to their critically small mesh size and inherent bioinertness. In this study, a Treg-regulated microgel scaffold (GTK-TK-drug) was prepared by inter-microgels assembly of microgels having cyclodextrin (CD) or adamantane (Ad) with a particle size of about 220 μm. The raw materials to obtain the microgels included gelatin methacryloyl (GelMA), reactive oxygen species (ROS)-responsive crosslinking agent poly(ethylene glycol)-thioketal-poly(ethylene glycol) (PEG-TK-PEG), ROS-responsive release prodrug aminooxyacetic acid (PEG-TK-AOA), PEG-CD or PEG-Ad, and photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP). Quantitative real-time PCR (qPCR), cell counting kit-8, and immunofluorescence staining revealed the effectiveness of AOA for the differentiation of T cells into Treg and the ingrowth of cells in vitro and in vivo . During the short treatment of MI in vivo , the levels of plasma inflammatory factors, cardiomyocyte apoptosis and inflammatory cells were reduced significantly. In the long-term animal experiments, the recovery of cardiac function was significantly enhanced as characterized by echocardiography and the degree of myocardial fibrosis compared to the unassembled microgels control.
研究领域:
生物材料
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心脏病学
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免疫学
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组织工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Subramani Kandhasamy,Baofang Wu,Jiayin Wang,Xiaojing Zhang,Gao Hongzhi,Da-Peng Yang,Yiming Zeng
Hydrogels incorporating natural biopolymer and adhesive substances have extensively been used to develop bioactive drugs and to design cells encapsulating sturdy structure for biomedical applications. However, the conjugation of the adhesive in most hydrogels is insufficient to maintain long-lasting biocompatibility inadequate to accelerate internal organ tissue repair in the essential native cellular microenvironment. The current work elaborates the synthesis of charged choline-catechol ionic liquid (BIL) adhesive and a hydrogel with an electronegative atom rich polyphenol (PU)-laden gelatinmethacryloyl (GelMA) to improve the structural bioactivities for in vivo tracheal repair by inducing swift crosslinking along with durable mechanical and tissue adhesive properties. It was observed that Bioactive BIL and PU exhibited potent antioxidant (IC 50 % of 7.91 μg/mL and 24.55 μg/mL) and antibacterial activity against E. coli , P. aeruginosa and S. aureus . The novel integration of photocurable GelMA-BIL-PU revealed outstanding mechanical strength, biodegradability and sustained drug release. The in vitro study showed exceptional cell migration and proliferation in HBECs, while in vivo investigation of the GelMA-BIL-PU hydrogel on a rat's tracheal model revealed remarkable tracheal reconstruction, concurrently reducing tissue inflammation. Furthermore, the optimized GelMA-BIL-PU injectable adhesive bioink blend demonstrated superior MSCs migration and proliferation, which could be a strong candidate for developing stem cell-rich biomaterials to address multiple organ defects.
研究领域:
生物医学工程
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组织工程
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干细胞生物学
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材料科学
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药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Wenjiao Ge,Shan Cao,Han Yu,Xiaohui Wang
Hydrogels are highly sought-after absorbent materials for absorbent pads; however, it is still challenging to achieve a satisfactory balance between mechanical performance, water absorption capacity, and active functionalities. In this work, we presented double-network hydrogels synthesized through acrylic acid (AA) polymerization in the presence of quaternized cellulose nanofibrils (QCNF) and Fe 3+ . Spectroscopic and microscopic analyses revealed that the combined QCNF and Fe 3+ facilitated the formation of double-network hydrogels with combined chemical and physical crosslinking. The synergistic effect of QCNF and Fe 3+ resulted in impressive mechanical properties, including tensile strength of 1.98 MPa, fracture elongation of 838.8 %, toughness of 7.47 MJ m −3 , and elastic modulus of 0.35 MPa. In comparison to the single-network PAA hydrogel, the PAA/QCNF/Fe 3+ (PQFe) hydrogels showed higher and relatively stable swelling ratios under varying pH levels and saline conditions. The PQFe hydrogels exhibited notable antioxidant activity, as evidenced by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and demonstrated effective antibacterial activity against both Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). These hydrogels show promising potential as an absorbent interlayer in absorbent pads for active food packaging.
研究领域:
材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Zhipeng Ni,Haiying Zhou,Haojie Yu,Li Wang,Chenguang Ouyang,Jian Yang,Yanzhao Dong,Ahmad Alhaskawi,Tian Tu,Hui Lu
The bidirectional regulations of antiadhesion and regeneration are crucial for Achilles tendon injuries. However, the precise prevention and treatments of peritendinous adhesions both in time and space are still great challenges. Here, a series of time–space regulating prodrug (tannic acid (TA), epigallocatechin (EGC) and epigallocatechin gallate (EGCG)) hydrogels are designed for the prevention of peritendinous adhesion in Achilles tendon contusion and rupture injuries. In the term of space, the self-healing and deformable hydrogels prepared by crosslinking phenylboric acid (PBA) functionalized polyphosphazene (PZBA) with guar gum (GG) through ROS-responsive phenylborate ester (PBAE) can specifically aggregate to the high ROS sites via the ROS-responsive degradation and recombination of PBAE. Thus, providing support and acting as barriers to inhibit fibroblast proliferation. In the term of time, the prodrug strategy using ROS-responsive PBAE as bridging linker can achieve long-term anti-inflammatory effect over 2 weeks. Furthermore, the degradation time of hydrogels matched the course of peritendinous adhesion, thus achieved bidirectional regulations (decrease the gene expression of TGF-β1, COL-1 and COL-3 to antiadhesion in the early stage but increase them to promote regeneration in the late stage). This study presents a promising approach employing smart prodrug hydrogels with time–space regulating properties for prevention of peritendinous adhesion.
研究领域:
生物医学工程
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药剂学
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材料科学
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组织工程
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Rui Wang,Xin Li,Chenglin Wang,Yu Shi,Ding Xiong,Dingming Huang,Zhenming Wang,Ling Ye
Cutaneous wound healing remains a common health problem. Metal-organic frameworks (MOFs) have emerged as an advanced therapeutic platform for promoted wound healing. However, there is a lack of MOF particles possessing excellent stability, biocompatibility, and reactive oxygen species (ROS) scavenging ability for tight orchestration of wound healing. Herein, we synthetize therapeutic MOF particles named PgC 3 Zn and employ them as skin sprays for wound repair. At the inflammatory stage, the pH- and ROS-responsive Zn 2+ release of PgC 3 Zn alleviates oxidative stress and exerts antibacterial and anti-inflammatory efficacy. During the proliferation stage, PgC 3 Zn promote the migration and proliferation of fibroblasts, the re-epithelialization of keratinocytes, and the angiogenesis of endothelial cells. During the remodeling stage, PgC 3 Zn effectively facilitate the wound closure and collagen deposition. Moreover, multiple endogenous growth factors have been identified to contribute to the wound healing process. Importantly, PgC 3 Zn exhibit excellent biocompatibility and remarkably accelerate the healing process in both acute and infected rat full-thickness skin wound models in vivo . Consistently, transcriptomic data illustrate the multi-stage and multi-functional regulation effects of PgC 3 Zn in promoting wound healing. This study proposes versatile and biocompatible PgC 3 Zn MOF particles with potentials for enhancing the management of acute and infected skin wounds.
研究领域:
医学
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材料科学
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生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
·
IF 8.5
Jianrui Qi,Jie Zhang,Kai Wang,Yifan Cheng,Qinglin Sheng,Ivan Kurtovic,Yahong Yuan,Tianli Yue
There is a lack of research on how Tibetan kefir grains fermentation alters the physicochemical properties and biological activity of Lycium barbarum pulp polysaccharides, despite some reports that fermentation can affect the structure and activity of plant polysaccharides. This study demonstrated that, through fermentation, the molecular weight of polysaccharides decreased from 25.33 to 15.11 kg/mol while the contents of total sugar and uronic acid increased by 19.11% and 40.38%, respectively. Furthermore, after fermentation, the polysaccharides exhibited an uneven and rough surface along with a reduced number of branched chains and triple helix structures. Tibetan kefir grains fermentation enhanced the antioxidant activity of polysaccharides, which may be attributed to an increase in arabinose, galactose, and uronic acid content and a decrease in polysaccharide molecular weight. This research offers an alternative viewpoint on the potential application of Tibetan kefir grains-fermented Lycium barbarum pulp polysaccharides in functional foods.
研究领域:
食品科学
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营养学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Chuwei Zhang,Xiaoyi Zhang,Fei Li,Bo Li,Mengnan Zhang,Wanqian Li,Pan Zhuge,Jingye Yao,Yi Zhang,Shixuan Chen,Yongjin Fang,Chao Cai
Effective regulation and reconstruction of the microenvironment are critical for the regeneration of chronic wounds. Diabetic wounds, in particular, pose a significant clinical challenge due to increased oxidative stress and dysfunctional healing processes. In this study, a novel therapeutic strategy is developed using 3D copper-magnesium bimetallic antioxidant nano-enzymes (Cu/Mg-MOF) to mitigate reactive oxygen species (ROS) and restore redox balance through electron transfer. To optimize delivery, a thermo-sensitive hydrogel composed of chitosan (CS) and ε -polylysine (PL) is designed, serving as an efficient carrier for the nano-enzymes. This Cu/Mg-MOF@CS/PL hydrogel exhibits excellent physical properties, including injectability, softness, and biocompatibility, making it ideal for application in diabetic wounds. In a diabetic wound model, treatment with Cu/Mg-MOF@CS/PL hydrogel significantly accelerated wound healing, with a closure rate of 90.6% by day 14, compared to just 55.4% in the untreated group. The hydrogel effectively promoted key aspects of wound healing, such as collagen deposition, re-epithelialization, angiogenesis, and immunomodulation. These findings underscore the potential of the Cu/Mg-MOF@CS/PL hydrogel as a promising therapeutic system for enhancing the healing of diabetic wounds.
研究领域:
生物材料学
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再生医学
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药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Linpin Luo,Meilin Wang,Wenqiao Su,Junchen Zhuo,Liang Zhang,Wenxin Zhu,Wentao Zhang,Rong Wang,Jianlong Wang
Antimicrobial packing showed great potential in extending the shelf life of food. However, developing a new biocomposite film with an intelligent and efficient antimicrobial performance is still desirable. Herein, a Fe-MoOx encapsulated with curcumin (Cur) filled chitosan-based composite film (CCF films) was prepared by solvent casting method. The total color differences of the CCF films were less than 30%, and satisfactory surface color, transparency, hydrophobicity, and thermal stability were also obtained. Besides, the UV-light/water/oxygen barrier capability and mechanical properties were enhanced with the incorporation of Cur@Fe-MoOx. Moreover, CCF films showed photothermal performance and thermal-controlled curcumin release ability, which endowed the CCF0.15 film with excellent antibacterial capability toward E. coli (≥99.95%) and S. aureus (≥99.96%) due to the synergistic antibacterial effect. Fe-MoOx exhibited high cell viability and less than 5% hemolysis even under the concentration of 500 μg mL–1. Based on those unique characteristics, the CCF0.15 film was chosen for tangerine preservation. The CCF0.15 film could prolong the shelf life of tangerine by at least 9 days compared with the unpacking group, and the tangerines could maintain the freshness characteristics over a 24 day storage period. Such thermal-mediated antibacterial film proposed by our work showed promising potential in food packaging.
研究领域:
食品科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）