VETERINARY MICROBIOLOGY
·
2025
·
IF 2.7
Qing Yang,Shengda Xie,Yundi Zhao,Xinxin Lin,Ning Wei,Miaolei Shi,Ruibing Cao
Japanese encephalitis virus (JEV) is a zoonotic pathogen that can be transmitted to humans and animals via arthropods, causing viral encephalitis. In this study, we investigated the role of transferrin receptor 1 (TfR1) in JEV infection. We found that knocking down TfR1 expression in A549 and HeLa cells significantly inhibited JEV entry, while overexpression of TfR1 in 293 T cells enhanced viral entry. The JEV E protein interacts with TfR1, specifically via the protease-like domain (residues 384–606). Additionally, the hTfR1 soluble ectodomain protein significantly blocked JEV infection in HeLa cells, and ferric ammonium citrate (FAC), an iron supplement, downregulated TfR1 expression and effectively inhibited JEV entry and infection. These findings identify TfR1 as an entry factor for JEV and a potential antiviral target, suggesting that small molecule inhibitors like FAC could offer a promising antiviral strategy.
研究领域:
病毒学
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分子生物学
·
免疫学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
Cell Reports Physical Science
·
2024
·
IF 7.9
Xinyu Shen,Yihua Zhong,Jie Han,Jing Wei,Bowen Yang,Jihan Wang,Yuxuan Zhang,Ruiran Li,Chunlin Li,Lixue Tang,Xu Zhang
Patients with lower urinary tract dysfunction are often unable to sense urine volume. Implantable sensors are used to monitor bladder urine volume. Securing sensors to the bladder involves sutures or synthetic adhesives that are invasive to it. Inspired by the safety harness that can be attached to objects by straps, we propose the bladder electronic harness (E-harness) for urine volume monitoring. The E-harness is a non-stick 2-dimensional structure that can be wrapped around the bladder without harming it. It is also soft enough to put no extra pressure on the bladder when it expands. Integrated with soft strain sensors and a wireless system, the E-harness can conform to the bladder, can measure bladder liquid volume with high accuracy (∼0.16 mL), and does not fall off for 1 month. The size of the E-harness is easily adjustable and can be used on small animals such as mice and large animals such as pigs.
研究领域:
生物医学工程
·
泌尿科
·
医疗器械
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DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 22
·2604-2613
·
IF 2.073
Xianxin Qiu,Longjie Zhong,Ying Gan,Kaiqi Su,Shihui Hu,Ping Wang
Okadaic acid is a kind of fat-soluble diarrhetic shellfish poison existing extensively in shellfish. The rapid on site detection of shellfish contaminated by the toxin is a serious problem. Herein, a new method combining a kit with the Bionic e-Eye was developed for the rapid detection of okadaic acid on site. After optimization the kit reached a very low limit of detection, 0.19 μg L−1, and the linear detection range was 0.2–10 μg L−1. The cross reaction rate with dinophysistoxins 1 was 23.6%. The kit had a small cross reaction with other kinds of toxin like saxitoxin, gynotoxin 2 & 3 and pectenotoxins 2-b. The recovery range was about 75.78–102.56%, and the CVs were lower than 20%. A correlation analysis was conducted between the Bionic e-Eye and a microplate reader in PBS and shellfish backgrounds. The detection results of the real shellfish samples by the Bionic e-Eye exhibited great consistency with high performance liquid chromatography. The total time taken to perform the detection was less than 90 minutes. Above all, the method integrated kit with the Bionic e-Eye was very suitable for the rapid detection of okadaic acid on site.
研究领域:
食品科学
·
分析化学
·
毒理学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Wei Ma,Binghua Yao,Wen Zhang,Yangqing He,Yan Yu,Jinfen Niu
A novel piezocatalytic active membrane based on mixed matrix of exfoliated multi-flaw MoS 2 (E-MoS 2 ) nanosheets and polyvinylidene fluoride (PVDF) was synthesized by electrospinning technique. The composition and structure of the obtained E-MoS 2 /PVDF electrospun fiber membranes (EFMs) were characterized via FESEM, HR-TEM, XRD, FTIR and XPS techniques. The piezocatalytic activity of the EFMs was evaluated by the degradation of antibiotic oxytetracycline (OTC) under ultrasonic irradiation in the dark. The 10.0 wt% E-MoS 2 /PVDF EFMs exhibited superior OTC degradation efficiency of 93.08% within 24 min with pseudo-first-order kinetic constant ( k ) of 0.09124 min −1 , which was 21.1 times higher than that of the pure PVDF EFMs. The superior performance was attributed to the synergetic effect of the embedded E-MoS 2 nanosheets and a high proportion of PVDF β -phase (94.1%) to effectively improve the piezoelectric property of composite. In addition, the ultrathin multi-flaw structure of E-MoS 2 provided abundant catalytic active edge sites and increased the surface charge density, promoting the generation of more ∙OH and ∙O 2 – radicals to degrade OTC pollutants. The degradation intermediates of OTC over 10 wt% E-MoS 2 /PVDF EFMs were detected by HPLC–MS and two main degradation pathways were reasonably proposed. The piezocatalytic activity of 10 wt% E-MoS 2 /PVDF EFMs remained stable after five consecutive cycles. This study is meaningful for exploring the composite membrane piezocatalysts for largescale applications in wastewater treatment.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2016
·
IF 2.667
Ping Wei,Jia-Xin Gao,Gao-Wei Zheng,Hong Wu,Min-Hua Zong,Wen-Yong Lou
The novel anti-Prelog stereospecific carbonyl reductase from Acetobacter sp. CCTCC M209061 was successfully expressed in E. coli combined with glucose dehydrogenase (GDH) to construct an efficient whole-cell biocatalyst with coenzyme NADH regeneration. The enzymatic activity of GAcCR (AcCR with a GST tag) reached 304.9 U/g-dcw, even 9 folds higher than that of wild strain, and the activity of GDH for NADH regeneration recorded 46.0 U/mg-protein in the recombinant E. coli . As a whole-cell biocatalyst, the recombinant E. coli BL21(DE3)pLysS (pETDuet- gaccr - gdh ) possessed a broad substrate spectrum for kinds of carbonyl compounds with encouraging yield and stereoselectivity. Besides, the asymmetric reduction of ethyl 4-chloroacetoacetate (COBE) to optically pure ethyl 4-chloro-3-hydroxybutyrate (CHBE) catalyzed by the whole-cell biocatalyst was systematically investigated. Under the optimal reaction conditions, the optical purity of CHBE was over 99% e.e. for ( S )-enantiomer, and the initial rate and product yield reached 8.04 μmol/min and 99.4%, respectively. Moreover, the space-time yield was almost 20 folds higher than that catalyzed by the wild strain. Therefore, a new, high efficiency biocatalyst for asymmetric reductions was constructed successfully, and the enantioselective reduction of prochiral compounds using the biocatalyst was a promising approach for obtaining enantiopure chiral alcohols.
研究领域:
生物工程
·
合成生物学
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DOI
PubMed
高影响因子
Frontiers of Materials Science
·
2025
·Vol. 19
·期 3
·1-11
·
IF 2.3
Sheng Yang,Qing Danni,Zhang Peng,Sun Yixin,Zhang Rong
Tetra-aminophenyl porphyrin (TAPP)-grafted Zn–Ag–In–S quantum dots (ZAIS QDs)/poly(maleic anhydride- alt -1-octadecene) (PMAO) nanoparticles were synthesized and their photoluminescence properties as well as photodynamic properties were studied. ZAIS QDs showed the brightest photoluminescence and highest quantum yield at an optimized Zn feeding molar ratio of 20%. Those TAPP-grafted nanoparticles (i.e., ZAIS/PMAO- g -TAPP) were able to produce 1 O 2 in aqueous solution under light irradiation as indicated by the 1 O 2 indicator, 9,10-anthracenediyl-bis(methylene) dimalonic acid (ADMA). ZAIS/PMAO- g -TAPP nanoparticles also demonstrate good biocompatibility and low dark toxicity even at a concentration as high as 2.8 mg·mL −1 , whith can be applied as both a fluorescence probe and a photodynamic therapy (PDT) agent. The PDT treatment showed that the viability of melanoma A2058 cells was less than 10% after treatment with the 420 nm light irradiation for 15 min at a photosensitizer concentration of 1.7 mg·mL −1 . During the PDT treatment with Escherichia coli , the survival rate of the bacteria decreased by −95% after light irradiation at the same concentration. Such dual-functional ZAIS/PMAO- g -TAPP nanoparticles researched in this study demonstrate promising potential for fluorescence labeling as well as effective PDT treatment against cancer cells and bacteria.
研究领域:
纳米技术
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光动力治疗
·
材料科学
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生物医学工程
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微生物学
·
肿瘤学
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DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2022
·Vol. 46
·期 3
·e16372
·
IF 2.609
Haixia Ye,Jinmin Wen,Shiyun Wang,Ying Zhang,Bingbing Wu,Siwei Liu,Xinru Chen,Yongqian Xiao,Chengtao Wang,Jianjun Hou,Ming Ni,Xian Xia,Yulin Li
Prolonging shelf life of fresh-cut water chestnuts (FCWC) by vitamin E (vit E) was investigated. Browning degree, decay degree, and edible quality (EQ) of FCWC were evaluated as evaluation indexes of shelf life. Meanwhile, total soluble solids concentration, titratable acid (TA) concentration, ascorbic acid (AA) concentration, hardness, ethylene concentration, polyphenol oxidase (PPO) activity, peroxidase (POD) activity, total phenols (TP) concentration, counts of thermophilic bacteria, yeasts, and molds colonies were analyzed. Shelf life of FCWC could be prolonged for 4 days by soaking them in 0.04% (v v −1 ) vit E anhydrous ethanol (1% [v v −1 ]) solution for 30 min. Vit E probably inhibited the increasing of PPO and POD activities, and the declining of TP concentration and hardness. Vit E had little effect on counts of thermophilic bacteria, yeasts, and molds colonies of FCWC. These results indicated that vit E could prolong shelf life of FCWC. Novelty impact statement Vitamin E (vit E) extents shelf life of fresh-cut water chestnuts for 4 days. Vit E inhibits browning by reacting with oxygen or by reducing polyphenol oxidase and peroxidase activities. Vit E has no effect on total soluble solids, titratable acid, ascorbic acid, and ethylene concentration.
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DOI
高影响因子
ACS Nano
·
2026
·
IF 17.3
Ruidong Xu,Tong Xu,Ming Li,Ganghua Li,Shukun Liu,Xinyang He,Bin Sun,Taotao Ai,Mingwei Tian
Flexible tactile-sensing devices are crucial for enhancing precision in wearable interaction electronics. However, traditional array-based sensing units encounter challenges in high environment robustness and spatial resolution. To address this limitation, we propose an array-free tactile-sensing E-textile that attains superior spatial resolution by exploiting non-faradaic junction effect between the E-textile and the human body. Upon finger contact, ions in the dermis redistribute directionally at the interface, creating a purely capacitive coupled nonfaradaic junction behavior that rapidly generates tactile-sensing signals (<40 ms). These signals demonstrate continuous, linearly graded, and complementary characteristics that enable accurate touch coordinate localization, allowing the E-textile to trace tactile trajectories (e.g., handwriting) at superior resolution without auxiliary integrated circuitry. Notably, the E-textile exhibits excellent robust performance under harsh conditions like extreme temperature (−30–80 °C, with error <0.0017%), mechanical damage, and cyclic washing (>50,000 cycle). Interestingly, this sensing approach can be extended to various mediums such as paper, wood, and water, showcasing its versatility for interactive applications. With its high spatial resolution, durability, and multimodal compatibility, the proposed E-textile offers a promising platform for next-generation human–machine interfaces.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Fangjie Liu,Zaifu Gong,HaiZhao Li,Wuhui Li,Yuliang Liu,Qingjie Wang
Proposes a functional modularization strategy to fabricate BZCY-GDC-LCO electrodes. • Exhibits low polarization resistance (0.141 Ω·cm 2 ) with 216 h stable operation. • Single cell delivers 0.757 W·cm −2 (FC) and 1.182 A·cm −2 at 1.3 V (EC) at 650 °C. Efficient triple-conduction (H + /O 2− /e − ) is vital for high-performance reversible protonic ceramic cells (R-PCCs), yet the underlying transport mechanisms and their specific impacts on electrode kinetics remain elusive. In this work, we employ a “functional modularization” strategy to engineer a BaZr 0.1 Ce 0.7 Y 0.2 O 3−δ -Gd 0.1 Ce 0.9 O 1.95 -LaCoO 3 (BZCY-GDC-LCO) triple-phase composite air electrode using commercially available precursors. The synergistic effects and individual roles of the conducting species in optimizing electrochemical kinetics were systematically investigated. The optimized BZCY-GDC-LCO electrode (1:1:1 mass ratio) exhibits excellent chemical compatibility, high oxygen vacancy concentration, and a well-distributed triple-phase boundary. Consequently, it achieves a low polarization resistance of 0.141 Ω·cm 2 at 650 °C and demonstrates robust long-term stability over 216 h of operation. Single-cell tests deliver a peak power density of 0.757 W·cm −2 in fuel cell mode and a current density of 1.182 A·cm −2 at 1.3 V in electrolysis mode at 650 °C. Kinetic analysis reveals oxygen adsorption/dissociation as the rate-determining step, while excessive steam partial pressure inhibits oxygen transport via surface site passivation. This work provides a scalable design strategy for high-performance R-PCC air electrodes, demonstrating that superior performance and favorable techno-economic feasibility can be achieved using conventional materials. Download: Download high-res image (166KB) Download: Download full-size image
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DOI
高影响因子
Sustainable Materials and Technologies
·
2026
·
IF 9.7
Shafqat Ali,Sajjad Ali,Wenbo Yang,Shixuan Yan,Raheel Akram,Asif Shahzad,Zareen Zuhra
Gold recovery from waste remains challenging due to the lack of materials that can simultaneously coordinate, reduce and stabilize Au species. Herein, we report a hierarchical redox-engineered copper(II) benzenehexathiol metal-organic framework (BHT MOF) featuring six thiolate coordination sites, uniformly grown on a three-dimensional carbon nanotube sponge (BHT MOF@CNT sponge), for the selective recovery of waste gold ions and their direct conversion into catalytically active gold nanoparticles (Au NPs). The hybrid material exhibits high gold adsorption capacity of 647.9 mg g −1 at pH 1.0 and 50 °C, coupled with outstanding selectivity, showing only a 5–10% decrease in Au uptake in the presence of 11 competing metal ions and achieving a total gold recovery efficiency exceeding 90% in both binary and multicomponent systems. The conformal growth of the BHT MOF on the conductive CNT sponge generates a hierarchical porous architecture with sulfur-rich active sites that provide strong Au-S coordination and thermodynamically favorable binding. Simultaneously, the redox-active thiolate ligands facilitate spontaneous in situ electron transfer, driving the reduction of Au(III) to metallic Au(0) and enabling the formation of uniformly dispersed Au NPs confined onto the hybrid matrix, without the need for external reducing agents or post-synthetic treatments. The resulting Au-BHT MOF@CNT catalyst exhibits an enhanced hydrogen generation rate and excellent cycling stability, benefiting from the synergistic interplay between thiolate-based redox engineering, strong metal-support interactions and accelerated electron transport through the CNT network. This work demonstrates a scalable waste-to-energy platform that integrates waste gold recovery and hydrogen production within a single multifunctional material, providing a sustainable strategy for precious metal recycling and clean energy generation.
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DOI
高影响因子
Small
·
2025
·
IF 12.1
Mianyi Xiang,Yamin Liu,Ziyang Yang,Jinlei Jiang,Weicheng Wang,Yao Hu,Daxiang Cui,Qichao Li
Electronic noses (e-noses) have become indispensable analytical platforms for gas detection. However, conventional e-nose systems face significant limitations in portable and wearable implementations due to their bulk and high-power consumption. Herein, a single-sensor-based multifunctional e-nose system is reported by integrating a micro-electromechanical system (MEMS) gas sensor with a flexible printed circuit board (FPCB). Specifically, the ZnO-ZnSnO 3 raspberry-like microspheres (ZZSRM) are utilized as the gas-sensitive materials, and the gas selectivity of the sensor is enhanced through a dual-temperature modulation strategy. Additionally, a gas classification model based on the MiniRocket algorithm has been developed, enabling efficient feature extraction and low-complexity classification of response signals, thereby satisfying the real-time processing demands of embedded devices. Moreover, a silent communication method is proposed, which maps breathing frequency to Morse code for information transmission in specific scenarios. Experimental results demonstrate that the wearable system achieves high-precision classification and concentration prediction for eight volatile organic compounds (VOCs), while simultaneously enabling robust recognition of exhaled signals and instantaneous conversion of Morse code into legible alphabetic characters. By combining the gas sensor with artificial intelligence (AI) technology, this work establishes a multifunctional flexible e-nose that merges portable gas detection and silent communication, offering a novel technological framework for environmental monitoring and human-machine interaction.
研究领域:
生物医学工程
·
微机电系统
·
气体检测
·
人工智能
·
可穿戴设备
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DOI
PubMed
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Juan Duan,Muxi Chen,Mengyan Wang,Rui He,Ke Li,Lei Shi
Radiation-induced oral mucositis (RIOM) substantially compromises the efficacy of cancer treatment and patient survival, while current therapeutic approaches exhibit limited efficacy in addressing its multifactorial pathogenesis. Curcumin possesses multi-targeted pharmacological properties relevant to RIOM, including antioxidative, anti-inflammatory, and mucosal repair effects, rendering it an ideal therapeutic agent. This study developed a curcumin-based intelligent-responsive integrated therapeutic platform (Cur-VE-Zn@gel) via a hierarchical assembly strategy. Specifically, curcumin was covalently bound to vitamin E to form the active center, while its coordination with Zn 2+ facilitates self-assembly into stable polymeric structures. This design not only significantly enhanced the biosecurity of curcumin but also amplified its antioxidant, anti-inflammatory, and tissue repair capabilities, while exhibited stable DNA repair characteristics, thus establishing a comprehensive radioprotective barrier. This bioactive complex was further incorporated into an adhesive thermosensitive hydrogel consisting of chitosan and poloxamer, which enabled targeted delivery to oral mucosa and bypassed the low systemic bioavailability of curcumin. Comprehensive in vitro and in vivo evaluations demonstrated that the Cur-VE-Zn@gel platform effectively modulated DNA damage repair, oxidative stress, inflammatory cascades, and epithelial regeneration, thereby achieving synergistic multi-targeted therapy for the suppression of RIOM. Ultimately, this biomaterial-based strategy successfully addresses the key pharmacological limitations of curcumin through structural modification and targeted delivery, providing a highly promising, safe, and effective approach for the clinically challenging management of RIOM.
研究领域:
生物材料
·
纳米医学
·
放射肿瘤学
·
药学
·
口腔医学
·
药物递送
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）