ACS Applied Nano Materials
·
2024
·
IF 5.3
Cijun Shuai,Xingming Long,Yingxin Yang,Bingxin Sun,Zihao Zhang,Guoyong Wang,Shuping Peng
Bacterial infection is a severe challenge after artificial transplantation due to the lack of an antibacterial functional implant, which results in delayed healing or even transplant failure. Herein, a sonodynamic antibacterial strategy was proposed by integrating a BiFeO3/Ti3C2 heterojunction into a three-dimensional-printed poly-L-lactic acid scaffold. BiFeO3 can be stimulated using ultrasonic energy to generate carriers. Ti3C2, with good conductivity, can form an interfacial transfer channel with BiFeO3, accelerating interfacial charge transfer. Additionally, forming a Schottky barrier between BiFeO3 and Ti3C2 effectively suppressed electron backflow. The separation of electron–hole pairs was significantly enhanced, thus improving the yield of reactive oxygen species. The results demonstrated that the antibacterial scaffold exhibited antibacterial rates of 93 and 91% against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus exposed to ultrasound, respectively. Additionally, the poly(L-lactic acid)-BiFeO3/Ti3C2 scaffold possessed good biocompatibility and showed great potential for bone regeneration.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2024
·Vol. 32
·期 1
·1-13
·
IF 2.6
Song Jie,Wu Haodi,Niu Yuhua,Hui Baoli,Wang Youqian,Li Long
Soilless culture is gradually becoming a new direction in the development of agricultural cultivation. However, the synthetic substrates currently used suffer from the problems of being nutrient-less and non-degradable, which will ultimately lead to the misuse of chemical fertilizers and serious environmental pollution. In this study, L-Tyrosine(L-Tyr) was used as a nitrogen nutrient modifier, and L-Tyr-PCL-PCDL-PUF/KHA (L-Tyrosine-Polycaprolactone-Polycarbonate-Polyurethane Foam/Kalium Humate, LTPPPK) soilless substrate was prepared by using biodegradable polycaprolactone and potassium humate, a natural plant-stimulating hormone, as raw materials. The experimental results demonstrated that the incorporation of L-Tyr significantly enhanced the LTPPPK material's vesicular pore structure and morphology, characterized by an apparent density of 65.69 kg/m 3 , a porosity of 85.58%, a pore diameter of 0.96 ± 0.17 mm, a compressive strength of 0.015 MPa, and optimal thermal stability when the L-Tyr content was 3%. Additionally, L-Tyr enhanced the water absorption and retention capabilities, as well as the degradation performance of the material, achieving a maximum water absorption factor of 12.524 g/g. The degradation rate was found to be 10.95% after 6 months of degradation by N435 lipase, indicating that the material is environmentally friendly upon disposal. As a lettuce growing substrate, the LTPPPK material showed a 7.90% and 9.34% growth in root whisker and leaf width compared with the unmodified one after 42 days of lettuce growth, and the content of chlorophyll and soluble protein increased by 4.46% and 16.42%. It was shown that LTPPPK material can provide a certain amount of nitrogen and potassium for plant growth when used as lettuce cultivation substrate.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yaxian Chen,Huiting Wang,Jie Zhou,Dongxue Lin,Ling Zhang,Zhiqiang Xing,Qian Zhang,Lixin Xia
L-cysteine, an indispensable amino acid present in natural proteins, plays pivotal roles in various biological processes. Consequently, precise and selective monitoring of its concentrations is imperative. Herein, we propose a Surface-enhanced Raman Scattering (SERS) sensor for detecting L-cysteine based on the anti-aggregation of 4-mercaptobenzoic acid (4-MBA) and histidine (His) functionalized silver nanoparticles (Ag NPs). The presence of Hg 2+ ions can induce the aggregation of Ag NPs@His@4-MBA due to the unique nanostructures of Ag NPs@His@4-MBA, resulting in a robust SERS intensity of 4-MBA. However, in the presence of L-cysteine, the stronger affinity between L-cysteine and Hg 2+ reduces the concentration of free Hg 2+ , causing the dispersion of the aggregated functionalized Ag NPs and the reduction of the SERS signal intensity of 4-MBA. The developed SERS platform demonstrates excellent performance with a low detection limit of 5 nM (S/N = 3) and linear detection capabilities within the range of 0.01–100 μM for L-cysteine. Additionally, the method was successfully employed for the determination of L-cysteine in spiked serum samples, yielding recoveries ranging from 95.0 % to 108.1 % with relative standard deviations of less than 3.3 %. This study not only presents a novel approach for fabricating highly sensitive and specific SERS biosensors for biomolecule detection but also offers a significant strategy for the development and construction of SERS substrates using anti-aggregation design.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Marine Life Science & Technology
·
2025
·
IF 5.3
Wang Yan,Wang Zhen,Zhao Wen-Xiao,Yuan Xiao-Jie,Yu Yang,Wang Peng,Wang Min,McMinn Andrew,Zhang Yu-Zhong,Peng Ming,Fu Hui-Hui,Chen Xiu-Lan
Free trans -4-hydroxy-L-proline (T4LHyp), a non-proteinogenic amino acid, is mainly released from the degradation of collagen, hydroxyproline-rich glycoproteins, and some peptide antibiotics in nature. Although it has been known that some terrestrial bacteria utilize T4LHyp as carbon and nitrogen source via a T4LHyp gene cluster, which and how marine microorganisms catabolize T4LHyp still remains unclear. Here, five T4LHyp-utilizing marine bacterial strains, Halomonas sp. 5021, Salinicola sp. 4072, Alteromonas sp. 6022, Alteromonas sp. 5112 and Alteromonas sp. 30521, were isolated from hydrothermal vent sediment samples collected from the southwest Indian Ocean. While Halomonas sp. 5021 can utilize T4LHyp as both a nitrogen and carbon source, the other four strains can utilize T4LHyp as only a nitrogen source. Then, the T4LHyp catabolic mechanisms of Halomonas sp. 5021 and Salinicola sp. 4072, as a representative of the four strains, were further investigated by genomic, transcriptional, and biochemical analyses. Halomonas sp. 5021 adopts an intact T4LHyp gene cluster containing four enzymes to catabolize T4LHyp into NH 3 and α-ketoglutarate to provide nitrogen and carbon sources for its growth. Compared to Halomonas sp. 5021, Salinicola sp. 4072 lacks an α-KGSA dehydrogenase gene in the T4LHyp gene cluster and can only catabolize T4LHyp into NH 3 and α-ketoglutarate semialdehyde to provide a nitrogen source for its growth. Bioinformatic investigation showed that the 5021-like and 4072-like T4LHyp gene clusters are predominantly found in bacteria from Pseudomonadota , which are widely distributed in multiple marine habitats. Thus, Pseudomonadota bacteria are likely the dominant group to drive the recycling and mineralization of T4LHyp in the ocean.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Fumin Xue,Xinyue Yuan,Fengying Wu,Jingling Liu,Yan Cheng
L -Abrine (N-Methyl- L -tryptophan, L-ABR) shows antioxidant and radical scavenging abilities in vitro but has low solubility. This research introduces a new L -abrine-oxalate (L-ABR-OXA) salt using grinding and solvent evaporation techniques. The structure was confirmed by single-crystal X-ray diffraction (SXRD), and its purity and stability were validated through PXRD, TGA, DSC, and FTIR analyses. A key innovation is the significant increase in solubility of L-ABR (4 times in water and 3 times in PBS) achieved through oxalate salt formation. This enhancement is backed by molecular electrostatic potential (MEP) and Hirshfeld surface analysis, which proved that hydrogen bonds are the primary factor driving the formation of salts. Antioxidant tests showed that L-ABR-OXA has superior radical scavenging and antioxidant activity at low concentrations compared to pure L-ABR, indicating its potential as a more effective bioactive formulation. This study offers a crystallography-based approach to improve the solubility and bioactivity of poorly soluble active pharmaceutical ingredients (API).
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2024
·
IF 4.1
Wang Miaomiao,Shen Qiuyu,Pang Jianyu,Mao Yu,Li Xiaofei,Tao Yanxia,Tang Wenru,Sun Ruifen,Zhou Xuhong
Objective: Carnation is a plant that holds high value in terms of its edibility, medicinal properties, and ornamental appeal. Creating no sense he aim of this study was to evaluate the antioxidant and antitumor properties of extracts derived from various parts of the carnation plant. Metabolomics technology was employed to identify the primary chemical constituents.Methods: Initially, we measured the total phenolic and total flavonoid contents in carnation roots, stems, leaves, and flowers, followed by assessing the antioxidant and anti-tumor capabilities of each component using diverse experimental methods. Subsequently, UPLC-MS/MS was employed to identify metabolites in different parts of carnation and investigate their roles in antioxidant and anti-tumor activities.Results: Mention numerical value- for better underatnding- Results of the study indicated that the methanol extract obtained from carnation flowers and roots exhibited superior antioxidant capacity compared to that from the stems and leaves. This disparity may be attributed to the abundance of polyphenols, flavonoids, and antioxidants present in the flowers, including methyl ferulate and luteolin-4’-O-glucoside. Furthermore, the significant presence of the anthraquinone compound rhein-8-O-glucoside in carnation roots may contribute to their enhanced antioxidant properties. Ten distinct compounds were isolated and recognized in carnation flowers, with Isoorientin 2”-O-rhamnoside and Kurarinone demonstrating notable antioxidant activity and binding affinity to SOD1 and SOD3, as validated through antioxidant screening and molecular docking.Conclusion: Overall, the findings from this study have expanded our knowledge of the phytochemical composition across different anatomical regions of the carnation plant, providing valuable insights for its holistic utilization.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yong Zhang,Hongyu Jing,Bo Meng,Xiaohong Qian,Wantao Ying
The facile enrichment of glycopeptides or glycoproteins poses great challenges for glycoproteomic research. In this study, a novel hydrophilic material, named zwitterionic hydrophilic L-cysteine derivatized straticulate-C 3 N 4 composites (LCAC), were synthesized and evaluated for the enrichment of N-glycopeptides. LCAC exhibited good biocompatibility, excellent hydrophilicity and selectivity, by virtue of the large surface of C 3 N 4 and the zwitterionic property offered by cysteine. LCAC demonstrated excellent performance for N-glycopeptide enrichment with the sensitivity of 0.033 fmol/µL, selectivity of 1:100, and high recovery rate (∼85%). The performance of LCAC was demonstrated by the identification of 35 N-glycopeptides from IgG, as well as capturing 1809 human urine N-glycopeptides corresponding to 876 N-glycoproteins. Comparing the LCAC with our developed phenylboronic acid functionalized material showed a certain complementary due to the different binding mechanism. The simple production and enhanced hydrophilic properties make the material a promising choice for glycoproteomics researches.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL RESEARCH
·
2015
·
IF 0.633
Fang-Ming Wang,Dan Bao,Bing-Xiang Hu,Ze-Yu Zhou,Deng-Deng Huang,Li-Zhuang Chen,Yuan-Yuan Dan
A series of novel 10-substituted 3,6-diphenyl-9-aryl-3,4,6,7,9,10-hexahydroacridine-1,8(2H,5H)-dione derivatives synthesised by condensation of different amines with xanthenes, was prepared from 5-phenylcyclohexane-1,3-dione and aromatic aldehydes by a Knoevenagel and Michael reaction and cyclisation catalysed by a trace of l-proline at room temperature. In addition, the molecular structure of a xanthene was determined by single-crystal X-ray diffraction analysis.
研究领域:
有机化学
·
药物化学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 23
·2676-2680
·
IF 2.073
Zili Huang,Yufang Yang,Yijuan Long,Huzhi Zheng
The concentration of L-cysteine (L-Cys) provides important information about specific physiological functions and disease diagnosis. In this work, we provided a facile colorimetric approach for L-Cys detection with high sensitivity and specificity. Ficin acted as a peroxidase mimic and catalyzed the chromogenic reaction of TMB and H2O2. However, after the introduction of L-Cys, L-Cys competed with TMB for H2O2. The competition is generally won by the more-reactive L-Cys resulting in fading of the chromogenic reaction. The detection limit of L-Cys can be as low as 20 nM (3σ). In addition, the assay can also be successfully applied to detect L-Cys in biological fluids, indicating its potential in biological sample analysis. Our work could promote the application of the enzyme mimicking activity of ficin in medical diagnostics and biochemistry.
研究领域:
生物化学
·
医学诊断
·
酶学
✓ 已验证
DOI
高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 13
·135503
·
IF 3.953
Shuyang Hu,Qiuyan Shuai,Yulong Lin,Yan Fu,Meng Li
L-3,4-dihydroxy-phenylalanine (L-dopa) is the most widely used drug in Parkinson's disease treatment. However, development of cost-effective and high-throughput sensors to accurate enantioselective discrimination of L-dopa and D-dopa remains challenging to date. Herein, on the basis of the peroxidase-mimic activity of chiral FexCuySe nanoparticles, we demonstrated a novel colorimetric sensor for determination of chiral dopa. The surface chiral ligand, L/D-histidine (L/D-His), endowed the nanozymes with enantioselectivity in catalyzing the oxidation of dopa enantiomers. According to the values of kcat/Km, the efficiency of L-His modified nanoparticles (L-FexCuySe NPs) towards L-dopa was 1.56 times higher than that of D-dopa. While, D-His can facilely reverse the preference of the nanozyme to D-dopa. On the basis of high catalytic activity and enantioselectivity of L-FexCuySe NPs in oxidation of L-dopa, the L-FexCuySe NPs-based system can be utilized for detection of L-dopa. The linear ranges for L-dopa determination were 5 μM–0.125 mM and 0.125 mM–1 mM with a detection limit of 1.02 μM. Critically, the developed sensor has been successfully applied in the quality control of clinical used L-dopa tablets. Our work sheds light on developing simple and sensitive chiral nanomaterials-based sensors for drug analysis.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2022
·
IF 7.139
Chao Chen,Xiaohua Deng,Yingjie Deng,Lu An,Yijie Deng,Yuying Zheng,Dai Dang,Xu Yang
Developing high-efficiency bifunctional materials for electro-catalysis and supercapacitors are urgently needed but challenging. Herein, we develop a self-supporting Co–Ni LDH electrode prepared by in-situ growing ZIF-L(Co)@ZIF-L(Co, Ni) on carbon paper followed by a pseudomorphic transformation. The optimized Co–Ni LDH/carbon paper electrodes (CN-2/CP) exhibit excellent electrochemical activity and stability in oxygen evolution reactions (OER) and supercapacitors. The CN-2/CP electrode displays a low overpotential of 230 mV at 10 mA cm −2 and superior stability at 40-h chronopotentiometry for OER. For supercapacitor, the CN-2/CP electrode delivers a high specific capacitance of 1346 F g −1 at 1 A g −1 and maintains a capacitance of 88.5% after 7000 charge/discharge cycles at 20 A g −1 . Based on the physical and chemical characterization results, the high performance originates from the in-situ electrochemical conversion of metal hydroxide , improved conductivity, fast charge transfer at the interface and unique layered cross morphology providing more active sites.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 4
·2556-2567
·
IF 3.612
Xiuyun Guo,Junjun Wu,Xiangren Meng,Yawei Zhang,Zengqi Peng
Summary This study investigated the effects of l -lysine (Lys) and l -histidine (His) on the oxidative characteristics and gel properties of porcine myofibrillar proteins (MP). Results showed that Lys and His had a strong ferrous ion-chelating ability and hydroxyl radical-scavenging activity. Moreover, Lys and His inhibited the protein carbonyl formation and MP aggregation at 0.2 M and 0.6 M NaCl, respectively, in a dose-dependent manner. Furthermore, 2 and 4 mg mL −1 Lys and His decreased the oxidation-induced loss of the tertiary structure of MP accompanied by the lower surface hydrophobicity. The water-holding capacity and gel strength of MP gels increased with increasing Lys and His concentrations due to more regular and lamellar structures with smaller and homogeneous pores at 0.6 M NaCl and more orderly crosslinking via fibrous filament at 0.2 M NaCl. In summary, Lys and His chelated the ferrous ions and scavenged hydroxyl radicals, decreased the oxidation-induced physicochemical changes, thus preventing oxidative damage during the formation of a three-dimensional gel network, which resulted in better gel quality.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）