ANALYTICA CHIMICA ACTA
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2025
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IF 6
Zhe Zhao,Bolong Liang,Ming Su,Shumin Lin,Shu-Xuan Liang,Zhaohan Liu,Jianwei Zhao,Mao Peng
Background Antibiotics have been extensively applied to treat human and animal diseases for a long time, leading to residues in the environment and food. As a major global concern, there is an urgent need for selective, sensitive, and easy-to-use detection techniques for antibiotics. The efficient extraction and enrichment of quinolone antibiotics in complex samples still subject to some limitations. Microporous organic networks (MONs) have received widespread attention as adsorbents for sample pretreatment. However, the design and synthesis of functionalized MONs to obtain superior performance still need to be developed. Results In this study, a magnetic functionalized triazine based-microporous organic network (Fe 3 O 4 @TMON) was synthesized for the extraction, purification, and detection of quinolone antibiotics in water and meat samples. Batch experiments, chemical characterization, and simulation calculations illustrated the good adsorption performance of Fe 3 O 4 @TMON for ofloxacin (OFL), ciprofloxacin (CIP), and enrofloxacin (ENR). π-π stacking, π-π EDA, hydrogen bonding, and hydrophobic interactions dominate the adsorption process. A novel method for detecting quinolones in water and meat samples using high-performance liquid chromatography with fluorescence detector was established under optimized magnetic micro-solid phase extraction parameters based on Fe 3 O 4 @TMON. For water samples, the enrichment factors, limits of detection, recoveries, and precisions were within the ranges of 215.8–265.0, 0.20–0.40 ng/L, 86.3-106.0%, and 2.9-7.9%, respectively. And for meat samples, they were 8.7-9.9, 0.08–0.16 μg/kg, 87.2-98.8%, and 2.9-8.6%, respectively. Significance This work developed a new member of MONs, exhibiting good application potential in the efficient extraction of organic pollutants from complex matrices. It provides a new perspective and reference for the formation of detection strategies, establishment of detection methods, and exploration of adsorption mechanisms. In addition, it ensures environmental safety and human health, laying a solid foundation for future scientific research and technological development.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yi Xing,Hanlin Xu,Min Zhang,Faliang Cheng
The excessive use of chlortetracycline (CTC) has the potential to adversely affect ecosystems and threat human health by inducing antibiotic-resistant infections, necessitating a rapid, sensitive and accurate detection method for CTC. Here, an electrochemical sensor based on molecularly imprinted polymer (MIP) has been developed that utilized poly o-phenylenediamine (poly-o-PD) film to specificity detect CTC, which preparation process was low-cost, time-saving and simple. It combines the recognition capabilities of MIP with the outstanding electrochemical performance of electrode modification materials, namely tungsten disulfide (WS 2 ) and carboxylated multi-walled carbon nanotubes (MWCNTs-COOH). And, in order to verified the optimal ratio of CTC (template) and o-PD (monomer), the density functional theory is performed by Vienna Ab Initio Simulation Package (VASP) within the generalized gradient approximation to calculate the interaction energy of CTC-o-PD. The sensor detects CTC by analyzing the current changes of the redox-active probes in response to the binding of CTC to the imprinted sites on the modified electrode surface. The sensor reveals a high affinity toward CTC binding, enabling the detection of CTC concentrations within the range of 0.5 – 425.0 μM, with a detection limit of 18.5 nM (S/N = 3). Meanwhile, the sensor harnesses the advantages of screen-printed electrode (SPE) such as miniaturization, suitability for large-scale production, and cost-effectiveness, to further enhance the practicality. The sensor described herein has been successfully used for specific quantitative detection of CTC in actual water, demonstrating its practical applicability.
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ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Kai Shi,Wenjie Luo,Ying Cheng,Honglei Li,Liai Peng,Xiangrui Luo,Yu Hu,Jiaheng Zhang,Jiaxuan Chen
CRISPR/Cas12a-powered biosensors with guanine (G)-rich sequence reporters (e.g., G-quadruplex and G-triplex) are widely used in detection applications due to their simplicity and sensitivity. However, when these biosensors are employed for molecular detection in complex samples, they may encounter difficulties such as high background signal and susceptibility to interference because of the “turn-off” signal output. Herein, we explore, for the first time, a set of phosphorothioate (ps)-modified G-quadruplex (G4) and G-triplex (G3) sequences that can bind with thioflavin T (ThT) in an active split CRISPR/Cas12a system (SCas12a) to generate a “turn-on” fluorescent signal. To apply this new phenomenon, we develop a universal SCas12a-powered biosensor for “turn-on” fluorescent detection of nucleic acid (miRNA-21) and non-nucleic acid (kanamycin) targets by using ps-modified hairpin G3 as a reporter (SCas12a/psHG3). Target recognition activates SCas12a’s trans-cleavage activity, leading to cleavage at the loop region of the psHG3 reporter. The released prelocked psG3 DNA binds ThT to produce a strong fluorescence signal. Without preamplification, this strategy can detect miRNA-21 with a detection limit of 100 fM. Moreover, the SCas12a/psHG3 system was further utilized for detecting kanamycin by incorporating its aptamers, enabling the detection of kanamycin at concentrations as low as 100 pM. This work is the first to develop a “turn-on” SCas12a/psHG3 system, showcasing its improved performance and wide range of applications in synthetic biology-based sensing technology.
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APPLIED SURFACE SCIENCE
·
2025
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IF 6.9
Kaiyue Yang,Pengfei Zhou,Lin Hao,Jinsheng Liang
Solar-driven photodegradation technology is a highly promising route for resolving environmental pollutants. However, the performance of photocatalysts is usually limited by the poor light harvesting, the high recombination of photogenerated electron-hole pairs, and the insufficient surface active sites. In this work, thin Bi 2 O 2 (OH)(NO 3 ) nanosheets co-modified with oxygen vacancies and bromine-grafting (BNSBr-OV) presented a superb rhodamine B photodegradation efficiency of 98.5 % within 4 h under visible light (λ＞420 nm), which was 8.2 times higher than that of pristine Bi 2 O 2 (OH)(NO 3 ). Besides, BNSBr-OV exhibited universal photocatalytic abilities of degrading various antibiotics, including tetracycline hydrochloride, ofloxacin and sodium sulfacetamide. The introduction of oxygen-vacancy and grafted bromine dual active sites can not only improve the light harvesting ability of the photocatalyst, but also promote the migration efficiency of photogenerated carriers, which boosts its photocatalysis performance. This study provides a platform for understanding the dual active sites modified single-crystal material, which potentially offers new design of environmental catalysts for multiple applications.
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JOURNAL OF CHROMATOGRAPHY A
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2018
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IF 3.716
Lili Lian,Xinyang Zhang,Jie Hao,Jinyi Lv,Xiyue Wang,Bo Zhu,Dawei Lou
In this study, a unique core-shell titanium-based metal-organic framework (MOF) functionalized magnetic microsphere was constructed and utilized as magnetic adsorbent for enrichment of five fluoroquinolones from water samples. The received magnetic MOF nanocomposite, denoted as Fe 3 O 4 @Cys@MIL125-NH 2 , showed strong magnetic responsiveness, and good hydrophilicity, as well as excellent affinity to the target analytes. Under optimal conditions, the developed magnetic solid-phase extraction (MSPE) method, combined with ultra-high performance liquid chromatography (UPLC), showed good linearity within the studied concentration range, and low limits of detection (0.05-0.2 μg L −1 ). The accuracy of the developed method was evaluated by measuring the extraction recovery of the spiked tap water and river water samples. The satisfactory recoveries of five fluoroquinolones were in the range of 83.8–109.4% with the RSDs lower than 8.9%. These results indicated that the as-prepared Fe 3 O 4 -Cys@MIL125-NH 2 is a promising magnetic adsorbent for efficient enrichment of fluoroquinolones from tap water and environmental water samples.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2020
·Vol. 22
·期 27
·15340-15353
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IF 3.43
Genxing Zhu,Jialu Zhu,Xinlong Fu,Qi Liu,Fengyi Cao,Yu-nan Li,Qi Qin,Mingli Jiao
In this study, Co nanoparticle-embedded N,O-codoped porous carbon nanospheres (C@Co) with abundant N and O doping, high graphitization, large specific surface area (319 m2 g−1) and a well-developed mesoporous structure were synthesized and characterized thoroughly, and were applied to activate peroxymonosulfate (PMS) for the degradation of methylene blue (MB). Various influential factors affecting the catalytic performance including C@Co dosage, PMS dosage, MB concentration, initial pH, temperature, and co-existing common anions and humic acid (HA) on the MB degradation were systematically investigated. The increase of the C@Co dosage (15–60 mg), PMS dosage (25–100 mg) and reaction temperature (278–308 K) promoted the MB degradation in the C@Co/PMS system. The best performance of the C@Co/PMS system was observed under weakly acidic or nearly neutral conditions. Both the MB concentration (25–100 mg L−1) and Cl− (5–100 mM), NO3− (10–500 mM), CO32− (10–300 mM), HCO3− (1–30 mM) and HA (2–40 mg L−1) had an inhibitory effect on MB degradation, and the degree of decrease in MB degradation increased as their concentrations were enhanced. Interestingly, HPO42− (1–100 mM) had an overall inhibitory effect on the degradation process of MB; however, in comparison with lower concentrations (1–10 mM), an attenuation of the inhibitory effect at higher concentrations (50–100 mM) could be observed. Moreover, the C@Co/PMS system also exhibited general applicability in eliminating various organic pollutants from water such as methyl orange, malachite green, safranine T, Congo red, Rhodamine B, ofloxacin and tetracycline. Classical radical-quenching tests and EPR measurements showed that both the non-radical pathway (major route, involving 1O2) and radical pathway (minor route, involving ˙OH, ˙SO4− and ˙O2−) contribute to the MB degradation. DFT calculations disclosed that the combination of Co–C interactions with graphitic N doping brought in catalytically active sites in C@Co where the charge states of some C atoms were significantly increased. The degradation intermediates of MB during the catalytic reaction were also identified by HPLC-MS and the possible degradation pathway was proposed. Overall, the resultant C@Co can be developed as a novel and efficient heterogeneous catalyst for activating PMS to degrade organic pollutants, and has potential application in environmental remediation.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2025
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IF 4.7
Huihui Su,Zeqian Ren,Peng Chen,Miao Yue,Na Sun,Jianhong Wang,Jizhou Wu,Yongming Fu,Jie Ma
The pervasive presence of antibiotics in natural water bodies has become a critical environmental and health concern. In this work, we have synthesized oxygen-doped graphitic carbon nitride (O-g-C 3 N 4 ) bulk via thermal condensation without any post processing, exhibiting a relatively high specific surface area of 42.053 m 2 g −1 and a reduced band gap to 2.56 eV. The O-g-C 3 N 4 bulk demonstrates the maximum piezo–photocatalytic degradation of ofloxacin (OFL) under blue-light LED irradiation, which perfectly matches with the maximum transmittance wavelength of water, minimizing light absorption loss during propagation in water. Taking advantages of oxygen-doping induced spin-polarization, the O-g-C 3 N 4 bulk achieves nearly complete (99.9 %) degradation of OFL within 12 min under blue-light LED illumination with 50 W power, and the complete degradation time further decreases to 6 min with the assistance of 80 W ultrasonication. The synergistic effect of combining photocatalysis with piezoelectricity is attributed that the ultrasound-induced piezoelectric field facilitates the generation of hydroxyl radicals and improves the separation of photogenerated electron–hole pairs. The findings contribute to sustainable solutions for antibiotic pollution challenges and advance the application of visible light piezo–photocatalysis.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Yiheng Yuan,Xishun Peng,Cunwei Wang,Haili Zhang,Caizhen Zu,Zhangyu Zhou,Zhongchen Bai
The threats of antibiotic residues to human health and the environment have become more and more severe due to the overuse of antibiotics in agriculture. Here, multi-walled carbon nanotubes (MWCNTs) were used to prepare MWCNTs@ZnO/Ag substrate by a simple two-step electrochemical deposition process to detect the enrofloxacin (ENR) residues in pork. The coral-like MWCNTs with a large surface area could promote ENR adsorption and provide more sites for growing the ZnO/Ag nanostructures. Moreover, ZnO/Ag nanostructures could not only facilitate the electron transfer to increase Raman intensity but also improve the stability of MWCNTs@ZnO/Ag substrate. Thus, the MWCNTs@ZnO/Ag substrate could easily recognize ENR molecules at a low concentration of 5 × 10 −10 M due to the stronger charge transfer effect between the substrates and analytes. In addition, the limit of detection of ENR residues in pork samples by using MWCNTs@ZnO/Ag substrate was up to 5.87 nM. The SERS sensor of the MWCNTs@ZnO/Ag substrate has a high sensitivity and long-term stability for the detection of antibiotic residues in food, which could be applied in fields of food safety.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Na Li,Shaobai Wen,Chunwei Yu
Sodium alginate (SA) hydrogels have been extensively utilized for pollutant removal from wastewater due to the distinct properties of hydrophilicity, biocompatibility and reusability. However, the limited functional groups and poor adsorption capacity have hindered their applications. In this study, a novel hybrid hydrogel bead, composed of sodium alginate, attapulgite, and zeolitic imidazole framework-8 (SA/ATP/ZIF-8), was synthesized using a one-pot blending and ion cross-linking method for adsorptive removal norfloxacin (NOR) from aqueous solutions. The incorporation of ATP and ZIF-8 significantly enhanced the thermal stability and anti-swelling properties of the SA hydrogel beads. The effects of solution pH, contact time, initial NOR concentration, temperature, and the presence of coexisting substances on NOR uptakes were systematically investigated. The results demonstrated that the NOR uptake on SA/ATP/ZIF-8 hybrid hydrogel beads was significantly higher than that of the pure SA and SA/ATP hydrogel beads, and the maximum theoretical adsorption capacity of SA/ATP/ZIF-8 for NOR was 1360.67 mg/g at pH 5.0. Furthermore, the adsorption kinetic was well-described by a pseudo-second-order model, while the adsorption isotherm was most accurately represented by the Langmuir-Freundlich model. In tests conducted with Nandu River water, the SA/ATP/ZIF-8 exhibited removal efficiencies of 100 % for NOR at an initial concentration of 0.0015 mmol/L and 71 % at 0.015 mmol/L. Spectroscopic characterization and adsorption performance experiments indicated that the adsorption of NOR onto SA/ATP/ZIF-8 involved multiple mechanisms, including hydrogen bonding, electrostatic adsorption, cation exchange, π-π electron donor-acceptor interaction (EDA), and coordination. Consequently, the remarkable features of SA/ATP/ZIF-8 hydrogel beads highlight their great potential and promising practicability for separation and removal of lower-concentration antibiotics from wastewater.
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ENVIRONMENTAL ENGINEERING SCIENCE
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2025
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IF 1.8
Jiahao Liu,Jun Zhang,Na Li
An environmentally friendly aerogel (CE/LN), composed of cellulose (CE) and lignin (LN) was synthesized via one-pot cross-linking method at room temperature, followed by freeze-drying. The CE/LN aerogel exhibited good mechanical properties and abundant functional groups, making it an effective adsorbent for the adsorption and removal of the antibiotic trimethoprim (TMP) from water. This study systematically investigated the effects of solution pH, aerogel dose, contact time, initial TMP concentration, temperature, ionic strength, and various water sources on TMP adsorption. The CE/LN aerogel demonstrated a higher TMP adsorption capacity of TMP than the CE aerogel across the entire pH range of 2.0–10.0. The adsorption process was well described by the Langmuir isotherm model and pseudo-second-order kinetic model. The CE/LN 6% aerogel achieved the highest adsorption capacity, with a theoretically maximal TMP uptake of 0.402 mmol/g at pH 6.0. However, high concentration of NaCl significantly reduced TMP uptake on the CE/LN 6% aerogel due to the electrostatic shielding effect. After five cycles of adsorption, the CE/LN 6% aerogel maintained a high adsorption capacity, retaining 80% of its initial uptake. Combined with the adsorption performance, the Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy analyses spectroscopy analysis before and after TMP adsorption, it can be concluded that the adsorption mechanism involved hydrogen bonding, electrostatic interactions, and π–π electron donor–acceptor interactions. Thus, the CE/LN aerogel, with its simple preparation method, good adsorption capacity, and reusability, shows great potential for widespread application in the removal of antibiotics from water.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Dingfeng Wang,Liwei Gao,Xinru Liu,Linfan Wang,Ting Zhang,Guoqiang Guo,Hongxing He,Da Chen
Trace detection of nitrite in food has gained increasing attention due to its connection with biological and environmental processes. Herein, a dual-mode fluorometric/colorimetric sensor is established for nitrite detection by combining the oxidase-like activity of iron alkoxide (IA) with the bimodal excitation properties of nitrogen-doped carbon dots (N-CDs). IA oxidizes the colorless 3,3′,5,5′-tetramethylbenzidine into blue-colored 3,3′,5,5′-tetramethylbenzidine oxide. Subsequently, nitrite further triggers a diazotization reaction, resulting in a color change from blue to green. Simultaneously, the fluorescence intensity of N-CDs at 470 nm decreases significantly, while the signal at 371 nm remains nearly unchanged, owing to the localized inner filter effect. Satisfactory results are attained with low limits of detection of 49 nM in the fluorometric mode and 73 nM in the colorimetric mode. Furthermore, an intelligent lateral flow assay platform is established for the visual detection of nitrite. Therefore, this work provides innovative guidance for nitrite detection in food safety.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiahong Pan,Zherui Wang,Jiajia Luo,Liwen Fan,Xinyun Li,Chenyi Wang,Yanqing Cong,Tong Wei,Shi-Wen Lv
Herein, a simple method for effective treatment of organic wastewater containing ofloxacin is proposed. Specifically, sulfur-doped Co 3 O 4 (abbreviated as S-Co 3 O 4 ) is first prepared and used then as a mediator to activate peroxymonosulfate (PMS). The sulfur doping can facilitate the conversion of low spin state Co to high spin state Co, thereby reducing the work function of Co 3 O 4 to improve internal electron escape probability. Meanwhile, the sulfur doping also induces to form more oxygen vacancies, decreasing interface resistance and accelerating charge transfer. As a result, the activation efficiency of PMS over S-Co 3 O 4 is enhanced. More importantly, the adsorption energy of PMS over S-Co 3 O 4 is reduced after the sulfur doping, leading to generate more S-Co 3 O 4 -PMS* complexes. Due to small activation energy, the S-Co 3 O 4 -PMS* complexes can be easily decomposed into SO 4 − and 1 O 2 . As predicted, the S-Co 3 O 4 -mediated PMS system shows excellent performance in ofloxacin degradation, and the removal efficiency is as high as 96.4 % within 20 min. In the S-Co 3 O 4 -2-mediated PMS system, the contribution rate of active species for ofloxacin degradation follows an order of SO 4 − > 1 O 2 > Co 4+ O > OH . Furthermore, the S-Co 3 O 4 -2-mediated PMS system with good stability exhibits strong removal ability towards other organic pollutants.
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