Food Bioscience
·
2025
·
IF 5.9
Mingjie Xia,Yangyang Zhang,Lina Yang,Hong Song,Jun Li,Guangchen Zhang,He Liu
Butyric acid is the main nutrient of intestinal epithelium, but it has an unpleasant odor. In order to increase the content of butyric acid in the intestine, soy hull polysaccharide (SHP) nanomicelle carrier was designed to deliver the precursor of butyric acid, tributyrin (TB). The SHP nanomicelles were characterized by laser particle size analyzer, XRD and AFM. And the effects of nanomicelles on the interfacial properties of the digestive system were investigated by simulating the gastrointestinal tract in vitro . The results show that SHP–TB nanomicelles are spherical. The peak value of C=O tensile vibration at 1650 cm −1 increased. The inclusion rate reached 30.44%, and the loading rate was 80.51%. The cumulative TB release rate was <20% after 2 h of release in the stomach and was <25% after 4 h of release in the small intestine, and the cumulative release rate in the colon reached >95%. With prolongation of fermentation, the OD 600 of the fermentation solution significantly increased, the pH and total sugar content significantly decreased, and total sugar utilisation rate >66.04%. The butyric acid content in the fermentation solution significantly increased and the proportion was above 0.4 on average, the average contents of total SCFAs increased from 2.04 mmol/L to 45.79 mmol/L, the interfacial tension decreased and tended to be stable at the late stage of interfacial adsorption. At a 1:0.5 mass ratio, the SHP–TB nanomicelles exhibited the best cell survival rate and colysis process, with no toxicity. Therefore, SHP nanomicelles can target TB delivery as an ideal carrier in the colon, and the intestinal environment is conducive to the degradation of TB into acid, promoting absorption in the body.
✓ 已验证
产品关联
DOI
高影响因子
Separations
·
2024
·Vol. 11
·期 6
·168
·
IF 2.5
Jianjun Wang,Bao Zhang,Hang Xun,Xi Yao,Feng Tang
Bamboo vinegar is a liquid biomass with a huge yield and complex chemical composition. At present, the relative quantification of bamboo vinegar has been investigated in most studies. To analyze twelve compounds from bamboo vinegar simultaneously, gas chromatography-mass spectrometry and an external standard method were used to develop an analytical method. In this method, chromatographic separations of all compounds were above 1.5. The linear range was between 0.100 and 10.000 mg/L, and the coefficient of determination (R2) was between 0.9981 and 0.9997, indicating a good linear relationship. The limit of detection (LOD) was between 0.004 and 0.780 mg/L; the limit of quantitation (LOQ) was between 0.016 and 3.120 mg/L; the relative standard deviations (RSDs) of instrument precision and method stability were less than 8%; the recovery rate was between 89.25% and 113.77%, and its RSD was between 0.44% and 5.70%. Using this method, fourteen bamboo vinegars and six wood vinegars were analyzed, and it was found that the content of propionic acid, phenol, and 2-methoxyphenol was higher in most samples. In addition, the differences in physicochemical properties between distilled bamboo vinegar and its original solution after atmospheric distillation were investigated.
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2025
·
IF 3.7
Yaqi Fu,Siyu Mao,Tianyue Liao,Wei Feng
The enzyme OleT can utilize H 2 O 2 as the co-substrate, and this biocatalysis is an H 2 O 2 -driven enzymatic catalysis. In this work, OleT was recombinated by being fused to an elastin-like polypeptide (ELP). The recombinant enzyme OleT-ELP exhibits higher stability and resistance to H 2 O 2 interference compared to native OleT. OleT-ELP showed improved catalytic efficiency in producing α-olefins via fatty acid decarboxylation. The recombinant 5-hydroxymethylfurfural oxidase ( HMFO-ELP) catalyzes the oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA), generating H 2 O 2 as a byproduct. Combining OleT-ELP with HMFO-ELP enabled simultaneous conversion of fatty acids and HMF. The in situ H 2 O 2 generated by HMFO-ELP was transferred to OleT-ELP, enhancing catalytic efficiencies for both α-olefins and FDCA production.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xinru He,Zhanpeng Zhang,Xuan Xuan,Tingyuan Tan,Jiaxuan Sun,Biao Wang,Yu Tian,Heng Chen
The application of chitosan in packaging has always been limited due to its brittle and hygroscopic nature. In this study, hydrophobic short-chain fatty acids (SCFAs) were utilized to modify chitosan to overcome this issue. For the first time, hydrophobic SCFAs, typically hexanoic acid and its homologs, were found to be able to dissolve chitosan in water as well as its hydrophilic analog. After water was removed through evaporation, hydrophobic SCFA-modified chitosan films were obtained. The results of mechanical testing and DSC analysis confirmed that these hydrophobic SCFAs could effectively plasticize the chitosan film. Moreover, water contact angle measurements revealed that the hydrophilicity of these plasticized chitosan films was significantly reduced. The findings from the moisture uptake experiments indicated that linear hydrophobic SCFAs reduced the hygroscopicity of the chitosan film. Additionally, the crystal structures, thermal and gas barrier properties, and antibacterial activities of these hydrophobic SCFA-modified films were systematically examined and compared with those of hydrophilic SCFA-modified chitosan. This study presents an innovative and practical method for modifying chitosan films by regulating the structure of the cosolvent, thereby facilitating the real-world application of chitosan-based food packaging materials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Huiling Tang,Qiuping Li,Zhengqi Zha,Yuzhi Jiao,Baowei Yang,Zhaoyan Cheng,Ting Wang,Hongping Yin
Ulcerative colitis (UC) is a chronic inflammatory disease resulting from abnormal immune response to gut microflora translocating through damaged intestinal barrier. Xylan acetate ester (XylA) can increase colon short-chain fatty acids (SCFAs) levels and alleviate kidney disease by inhibiting inflammation through the G protein-coupled receptor pathway. Here, we synthesized and purified XylA, and then the effects and mechanisms of XylA on dextran sodium sulfate-induced UC in mice were investigated. The results showed that in mice, similar to the positive drug 5-aminosalicylic acid, oral administration of XylA significantly alleviated all UC symptoms, including weight loss, diarrhea, and hematochezia. Further mechanism studies revealed that XylA could repair the damaged colon structure and intestinal barrier function by increasing the expression of tight junction protein zonula occludens 1 and occludin, thus reducing LPS penetration. Moreover, XylA could also restrain intestinal inflammation via inhibiting LPS-TLR4 pathway, downregulating M1 macrophage polarization, and reducing proinflammatory cytokines expression, and in vitro cell experiments showed that these effects may be mediated by XylA derived SCFAs, particularly acetates, propionates and butyrates. All these results suggested that XylA may be a potential improving agent for UC treatment, and natural polysaccharides may represent a novel avenue for drug development of UC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Sichen Li,Huihui Peng,Yuning Sun,Jiali Yang,Juan Wang,Fuqing Bai,Chuanyi Peng,Shuzhen Fang,Huimei Cai,Guijie Chen
Yeast β-glucan intervention offers a promising strategy for managing colitis; however, the mechanisms remain unknown. In the present work, the protective effects of yeast β-glucan on DSS-induced colitis in mice was evaluated, focusing on its interaction with gut microbiota. The result showed yeast β-glucan significantly alleviated colitis symptoms, evidenced by reduced weight loss, lower disease activity index (DAI) scores, and minimized intestinal damage. It enhanced intestinal barrier integrity via upregulation of tight junction proteins, suppressed lipopolysaccharide (LPS) release, and decreased pro-inflammatory cytokines production. Additionally, yeast β-glucan boosted short-chain fatty acids (SCFAs) production, and activated their receptors, increased the relative abundances of beneficial microbes like Lactobacillus and Lachnospiraceae_UCG-006 . Transcriptomic analyses suggest that yeast β-glucan mitigates inflammation by downregulating gene expression related to IL-17 pathway. Our findings highlight potential of yeast β-glucan as a therapeutic agent for colitis through modulation of gut microbiota and inflammatory responses.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Yuling Li,Weiran Zhao,Xiang Xiang,YunLei Hu,Xiaoqiang Zhang,Wenjian Sun,Xiaoxuan Liu
Background Perturbed lipid metabolism in cellular environment plays an essential role in the progression of peripheral neuropathy, such as Charcot-Marie-Tooth disease type 1A (CMT1A). Current deep-profiling of lipidome still has certain limitations for high-throughput analysis of clinical samples, such as low sensitivity, extensive requirement of instrument modification, technician-dependent pretreatment and lack of method-adapted data-processing software. Results Herein, a new generation aza-Prilezhaev aziridination (APA) reagent has been designed for coupling with high-resolution liquid chromatography mass spectrometry (LC-MS) to conduct comprehensive untargeted deep lipidomics of CMT1A disease. It facilitates large-scale lipid structural annotations over a wide polarity range in a single run with high-sensitivity. With the developed method, an extensive library containing information on retention time, m / z , and MS/MS down to C=C location levels for 393 unsaturated lipids of CMT1A was created. Significant disease-related differences in C=C isomer profiles were observed from the comparison of plasma between CMT1A patients (N=9) and controls (N=8). Specifically, 40 different lipids were identified, predominantly comprising n-6, n-7, and n-9 C=C positional isomers. Significance This innovative methodology would offer substantial potential to enhance diagnosis and therapeutic strategies for CMT1A and other diseases based on lipid isomerization information.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Kai-Xin Jiang,Jin-Lei Geng,Qin Yuan,Jie Li,Wen Liu,Hong-Yan Liu,Yi-Chen Hu,Liang Zou,Shengpeng Wang,Ding-Tao Wu
Thinned unripe kiwifruit, a significant byproduct of commercial kiwifruit production, contains abundant pectic polysaccharides comparable to mature kiwifruit. Although acidic heteropolysaccharides from mature kiwifruit exhibit potent anti-inflammatory activity, the protective effects of unripe kiwifruit-derived pectic polysaccharides against ulcerative colitis (UC) remain unclear, ultimately limiting their potential utilizations. Therefore, to bridge this knowledge gap and facilitate their utilization, the chemical structures and anti-UC effects of two pectic polysaccharides, TKP (from unripe kiwifruit) and MKP (from mature kiwifruit), were systematically studied and compared. Results demonstrated that TKP and MKP share nearly identical primary chemical structures, predominantly comprising homogalacturonan (HG) and rhamnogalacturonan I (RG-I) domains. Particularly, TKP exhibited a longer side chain length (14.37 vs. 8.21) and higher RG-I content (73.62 mol% vs. 50.02 mol%) than MKP. Furthermore, both TKP and MKP significantly alleviated dextran sulfate sodium (DSS)-induced UC in mice. Notably, TKP exhibited superior effects to MKP in several aspects, such as enhanced butyrate production, more effective restoration of goblet cell populations, enhanced upregulation of tight junction protein ZO-1, and greater reduction in pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α). Both TKP and MKP effectively suppressed pathogenic bacteria (e.g., Escherichia-Shigella and Oscillibacter ) while promoted beneficial bacteria (e.g., Akkermansia and Bacteroides ). Notably, TKP significantly and selectively enhanced Paraprevotella growth, an effect not observed within MKP. These differential effects between TKP and MKP may be linked to their structural differences. Collectively, these findings provide valuable insights to develop TKP as a promising functional food or ingredient for preventing and ameliorating intestinal inflammatory disorders.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wudan Cai,Shuai Chen,Xiao Zhang,Dan Wu,Xinyi Zhu,Honglin Zhu,Yangchao Luo,Qilin Huang
The current study aimed to investigate the effect of ultrasound assisted H 2 O 2 /Vc degradation on the digestive characteristics of Lignosus rhinocerotis polysaccharide (LRP) and gut microbiota regulation. Results showed that during in vitro digestion, no changes were observed in molecular weight ( M w ) of LRP and its antitumor activity remains stable. However, M w of degraded LRP (DLRP) slightly decreased from 45.66 × 10 4 Da to 36.61 × 10 4 Da with no free monosaccharides released, enhancing its antitumor activity. The supplementation with LRP or DLRP significantly elevated mice fecal water content and modulated microbiota composition. The total short chain fatty acids concentration of control, LRP, and DLRP groups were 20.07, 24.13, and 28.08 mmol/L, respectively. Compared with LRP, DLRP significantly increased the abundance of beneficial bacteria such as Alloprevotella and Mucispirillum , exhibiting protective effects on intestinal health. This study provides a theoretical foundation for targeted regulation of intestinal flora by LRP and DLRP.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Mengqing Yan,Guoyu Liu,Shiwei Liu,Jia Liu,Haizhi Li,Haotian Wang,Yan Zou,Cong Pan,Fang Zhou,Xueying Zeng,Youqiang Yu,Yimin Wu,Shuheng Yang,Shenglin Duan,Peng Yuan
Yeast wall polysaccharides (YWPs) are a bioactive compound found in the vegetative cells of Saccharomyces cerevisiae , contributing to its applications in food and medicine. YWPs have been extensively studied because of their diverse biological activities. Although the yeast spore wall of S. cerevisiae also contains polysaccharides, the biological activities of the components of the spores have received limited attention. In this study, yeast spore wall polysaccharides (YSWPs) were extracted from chs 3Δ spores with exposed β-glucan layers via an ultrasonic-assisted enzymatic extraction (UAE) method, and the optimal extraction process was determined through single-factor and orthogonal experiments. The physicochemical properties of YWPs and YSWPs were compared under identical extraction conditions, and the effects of YSWPs on the abundance of the intestinal microflora and short-chain fatty acids (SCFAs) production in vitro were investigated. The results revealed that the optimal UAE process for YSWPs involves an ultrasonic power of 300 W, an ultrasonic duration of 60 min, an enzyme concentration of 1 % (w/w), and an enzymatic hydrolysis time of 4 h. The total sugar content of the extract was found to be 89.20 ± 0.52 %. The primary monosaccharides present in YSWPs are mannose and glucose, which are consistent with those found in the vegetative cell wall polysaccharides. Both YWPs and YSWPs exhibit similar physical properties, however, YSWPs has a smaller particle size, resulting in superior water-holding and oil-holding capacities. In simulations of in vitro colon fermentation, YSWPs can increased the population of beneficial microorganisms in the human intestine while reducing the number of harmful microbial populations. Additionally, it can effectively produced propionate and butyrate. These findings suggest that YSWPs can help maintain the intestinal ecological balance and promote intestinal health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROGASTROENTEROLOGY AND MOTILITY
·
2024
·
IF 3.5
Lei Chen,Xiangbing Chang,Chuntao Wu,Guofu Luo,Peifeng Zhang,Wei Tian
Background The traditional herbal medicine Atractylodes macrocephala Koidz. ( A. macrocephala ) is commonly utilized for alleviating symptoms associated with spleen deficiency, abdominal distension, diarrhea, and constipation. These pharmacological effects are attributed to a variety of active constituents. However, the specific bioactive compounds responsible for promoting defecation and gastrointestinal transit in A. macrocephala remain unidentified. Methods The primary polysaccharide characteristics of PAMK was elucidated by HPLC, FT-IR, and HGPGC. Efficacy of PAMK (0.07, 0.14, and 0.28 mg/g) on mice was evaluated in a spleen deficiency constipation mouse model by analyzing stool parameters, constipation-related physiological indexes, and SCFAs. The expression levels of 5-HT 3 R, 5-HT 4 R, and related receptor genes were examined by RT-qPCR, and neurotransmitters were examined using ELISA. Finally, the diversity of gut microbiota was analyzed with 16S rDNA sequencing. Key Results The results showed that PAMK significantly reduced the gastrointestinal transport time and increased the number of fecal pellets and fecal water content in spleen deficiency constipation model mice. PAMK kept the balance of 5-HT, SCFAs, TPH-1, SERT, CgA, and neurotransmitter levels (VIP, SP, MTL) in mice colon. In addition, PAMK could regulate the abundance of gut microbiota such as Alistopes , Bacteroides , and Odoribacter in spleen deficiency constipation model mice gut. Conclusions and Inferences It can be concluded that PAMK effectively ameliorated the symptoms of spleen deficiency constipation in mice by modulating the expression of 5-HT and its associated receptors. The underlying mechanism was elucidated, providing a solid theoretical foundation for the therapeutic application of A. macrocephala in treating spleen deficiency constipation and offering potential for developing novel approaches to address this condition.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Carbohydrate Polymer Technologies and Applications
·
2025
·
IF 6.5
Kit-Leong Cheong,Amanullah Sabir,Suresh Veeraperumal,Franck Quero,Rui Li,Qiaoli Zhao,Karsoon Tan,Saiyi Zhong,Udayakumar Veerabagu
This study investigates the esterification of laminarin, a β-glucan polysaccharide, using trifluoroacetic anhydride and acetic acid to synthesize laminarin acetyl esters (LA) with potential therapeutic properties. The efficacy of LA was evaluated in an in vivo dextran sulfate sodium induced colitis mouse model. Treatment with LA significantly improved the organ index and modulated inflammatory responses by upregulating anti-inflammatory cytokines, such as IL-10 and TGF-β1. LA also promoted a favorable shift in gut microbiota composition, notably increasing the relative abundance of beneficial bacteria like Lactobacillus and Bifidobacterium , and enhancing short-chain fatty acid production, particularly acetic acid. Immunohistochemical analysis revealed a reduction in CD68 expression and an elevation in CD163 expression, indicating a shift toward an anti-inflammatory macrophage phenotype. These findings demonstrate that LA mitigates pro-inflammatory responses, enhances gut barrier integrity, and supports a healthy gut microbiota, underscoring its potential as a therapeutic agent for managing colitis and improving gut health.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）