Biosensors-Basel
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2025
·Vol. 15
·期 8
·477
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IF 5.6
Jiahui Tian,Xianhe Jiao,Jiaqi Guo,Qian Yu,Shuqin Zhang,Guizhou Gu,Kundan Sivashanmugan,Xianming Kong
The presence of polycyclic aromatic hydrocarbons (PAHs) in edible oil has a serious effect on human health and may potentially induce cancer. This study combined thin-layer chromatography and surface-enhanced Raman spectroscopy (TLC-SERS) to rapidly and quantitatively detect PAHs in culinary oil. Machine learning using the principle component analysis-back propagation neural network (PCA-BP) was integrated with TLC-SERS for the detection of PAHs. Ag nanoparticles on diatomite (diatomite/Ag) TLC-SERS substrate were prepared via an in situ growth process and employed as a stationary phase in the TLC channel. The analyte sample was dropped onto the TLC channel for separation and detection. The diatomite/Ag TLC channel demonstrated excellent separation capability and superior SERS performance and successfully detected PAHs from edible oil at a sensitivity of 0.1 ppm. The PCA-BP quantitative analysis model demonstrated outstanding prediction performance. This work demonstrates that the combination of TLC-SERS technology with PCA-BP is an efficient and accurate method for quantitatively detecting PAHs in edible oil, which can effectively improve the quality of food.
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FOOD CHEMISTRY
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2025
·
IF 9.8
Yaqi Liu,Zehui Zhu,Xin Ai,Fei Pan,Wenli Tian,Lei Zhao,Liang Zhao
This study aimed to develop a self-assembled nanodelivery system derived from sesame protein hydrolysates (SPH) for the efficient encapsulation and delivery of β-carotene (BC). SPH with a 12 % degree of hydrolysis (SPH DH12 ), prepared via alkaline protease-mediated hydrolysis, exhibited optimal self-assembly properties. Optimized parameters yielded 50.30 ± 0.43 nm BC-SPH DH12 nanoparticles with 75.23 ± 0.92 % encapsulation efficiency and 11.14 ± 0.14 % drug loading capacity. BC encapsulation triggered secondary structural shifts (33.8 % α-helix decrease, 41.9 % β-sheet increase) and masked characteristic FTIR peaks. Peptidomics integrated with coarse-grained molecular dynamics identified five core peptides for BC encapsulation via hydrophobic interaction-driven self-assembly. In vitro digestion showed BC-SPH DH12 effectively prolonged BC release (26.76 % cumulative release). Rat pharmacokinetic studies further confirmed superior absorption, with a peak plasma concentration of 64.98 ng/mL and 2.73-fold higher bioavailability versus free BC. This work confirms that SPH self-assembly represents an effective strategy for hydrophobic nutraceutical delivery.
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TALANTA
·
2014
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IF 3.511
Man Wang,Shihai Cui,Xiaodi Yang,Wentao Bi
An easy preparation of g-C 3 N 4 /Fe 3 O 4 nanocomposites by chemical co-precipitation has been demonstrated. The as-prepared materials were characterized by X-ray diffraction, transmission electron microscopy , Fourier transform infrared spectroscopy and thermogravimetric analysis . The high affinity of g-C 3 N 4 toward polycyclic aromatic hydrocarbons and the magnetic behavior of Fe 3 O 4 were combined to provide an efficient and simple magnetic solid phase extraction (MSPE). The adsorption and desorption of polycyclic aromatic hydrocarbons on g-C 3 N 4 /Fe 3 O 4 were examined. Different factors affecting the magnetic solid phase extraction of polycyclic aromatic hydrocarbons were assessed in terms of adsorption, desorption, and recovery. Under the optimized conditions, the proposed method showed good limits of detection (LOD, S/N=3) in the range of 0.05–0.1 ng mL −1 and precision in the range of 1.8–5.3% (RSDs, n =3). This method was also successfully applied to the analysis of real water samples; good spiked recoveries over the range of 80.0–99.8% were obtained.
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ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Na Liu,Yanan Tan,Yingwen Hu,Tingting Meng,Lijuan Wen,Jingwen Liu,Bolin Cheng,Hong Yuan,Xuan Huang,Fuqiang Hu
Redox-responsive nanomaterials applied in drug delivery systems (DDS) have attracted an increasing attention in pharmaceutical research as a carrier for antitumor therapy. However, there would be unwanted drug release from a redox-responsive DDS with no selection at nontarget sites, leading to undesirable toxicities in normal tissues and cells. Here, an A54 peptide modified and PEGylated reduction cleavable glucolipid conjugate (A54-PEG-CSO-ss-SA, abbreviated to APCssA) was designed for intracellular delivery of doxorubicin (DOX). The synthesized APCssA could be assembled via micellization self-assembly in aqueous water above the critical micelle concentration (54.9 μg/mL) and exhibited a high drug encapsulation efficiency (77.92%). The APCssA micelles showed an enhanced redox sensitivity in that the disulfide bond could be degraded quickly and the drug would be released from micelles in 10 mM levels of glutathione (GSH). The cellular uptake studies highlighted the affinity of APCssA micelles toward the hepatoma cells (BEL-7402) compared to that toward HepG2 cells. In contrast with the nonresponsive conjugate, the drug was released from APCssA micelles more quickly in 10 mM level of GSH concentration (tumor cells). Moreover, the DOX-loaded APCssA micelles displayed an increased cytotoxicity which was 1.6- to 2.0-fold that of unmodified and nonresponsive micelles. In vivo, the APCssA micelles had stronger distribution to liver and hepatoma tissue and prolonged the circulation and retention time, while the drug release only occurred in the tumor tissue. The APCssA/DOX showed the tumor inhibition rate equal to that of commercial doxorubicin hydrochloric without negative consequence. This study suggested that the APCssA/DOX showed promising potential to treat the tumor for its special tumor targeting, selective intracellular drug release, enhanced antitumor activity, and reduced toxicity on normal tissues.
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ENVIRONMENTAL RESEARCH
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2025
·
IF 7.7
Jianli Jia,Ben Zhang,Aoran Li,Weiran Wang,Bing Xiao,Xiaolong Gao,Haokun Yuan,Yuxin Han,Xiwang Zhao,Ravi Naidu
Microbial technologies hold great promise for in situ remediation of polycyclic aromatic hydrocarbons (PAHs)-contaminated soils. However, the selection of enhancement measures and corresponding remediation strategies remains insufficiently understood. In this study, a series of enhancement treatments, including bacterial consortium inoculation (comprising Achromobacter denitrificans BP1, Rhodococcus aetherivorans BW2, and Lysinibacillus sp. BS3), nutrient addition, and bio-ventilation, were implemented to develop effective in situ remediation strategies for PAHs-contaminated soil. Over a 60-day incubation, the enhancement treatments achieved phenanthrene (PHE) degradation efficiencies of 68.0–94.7 % and benzo[a]pyrene (BaP) degradation efficiencies of 12.9–82.4 %. Degradation rates across soil layers followed the pattern: upper layer > lower layer > middle layer. Enhancement treatments significantly boosted soil dehydrogenase (DH) and fluorescein diacetate (FDAH) activities. Among these, the sequential consortium inoculation with nutrient addition treatment (T6) demonstrated the highest degradation efficacy. In the treatment T6, the relative abundance of consortium genera was significantly increased, playing critical roles in PAHs degradation. The connectivity and stability of the soil bacterial network were enhanced, providing greater resilience to pollutants. Quantitative PCR analysis showed that the enhancement strategy increased RHDα-GN gene abundance by 1.98-fold at the initial and maintained a positive correlation with PAHs residues throughout the process (p < 0.05), and the phe gene exhibited a continuous upward trend during remediation, ultimately reaching 1.61–1.96 times its initial abundance. Overall, this study provides a strong candidate of integrated enhancement strategies to advance in situ bioremediation of PAH-contaminated sites.
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Polymers
·
2025
·Vol. 17
·期 12
·1716
·
IF 4.9
Lili Kong,Xinna Yu,Qifang Sun,Meizhen Huang,Tianyuan Liu,Jie Chen
Polycyclic aromatic hydrocarbons (PAHs) have attracted significant attention due to their severe threats to both ecological systems and human health. In this paper, a high-performance surface-enhanced Raman spectroscopy (SERS) substrate based on NCF/GO/Au@Ag nanocomposites was developed, which enabled sensitive and stable detection of PAHs. The NCF/GO/Au@Ag substrate synergistic utilizes the localized surface plasmon resonance (LSPR) effect of Au@Ag core–shell nanorods and the additional interfacial charge transfer provided by graphene oxide (GO) to exhibit extremely high sensitivity. And the three-dimensional fibrous network of nanocellulose (NCF) improved nanoparticle dispersion uniformity. Combined finite element simulations and experimental studies verified that the dual plasmonic resonances (512 nm and 772 nm) of Au@Ag nanorods optimally match 785 nm excitation, yielding an enhancement factor of 5.21 × 10 5 . GO integration enhanced Raman signals by 1.68-fold through interfacial charge transfer, while the introduction of NCF reduced the signal relative standard deviation (RSD) from 36.88% to 4.29%. The NCF/GO/Au@Ag substrate achieved a detection limit of 10 μg/L for PAHs, demonstrating exceptional sensitivity and reproducibility.
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IEEE Access
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2025
·
IF 3.6
Mahmoud A. Ali,Xingliang Jiang,Ran Li,Ahmed S. Menesy,Waleed M. Hamanah,Muhammad Khalid
The growing use of composite polymer insulators in high-voltage direct current (HVDC) transmission systems requires a thorough understanding of their dynamic hydrophobicity characteristics to ensure long-term reliability. This study investigates the retention, recovery, and transfer of hydrophobicity in silicone rubber (SiR) composites filled with two-dimensional (2D) nanosheets, specifically molybdenum disulfide (MoS2) and tungsten disulfide (WS2), under DC Dynamic Drop Test (DDT) conditions. The efficacy of these fillers is evaluated against traditional alumina trihydrate (ATH) and fumed silica (SiO2) fillers. The Weibull probability analysis of the hydrophobicity retention test indicates that MoS2-filled composites achieved the highest hydrophobicity retention time (773.4 min), representing a 324.3% increase compared to the unfilled sample (185.4 min), while WS2 composites showed a 223.1% increase (609.7 min). In terms of hydrophobic recovery after 24 hours, MoS2 composites demonstrated a 282.6% improvement (125.6 min), followed by WS2 with a 160.4% improvement ( $\eta =87.1$ min) relative to the unfilled reference (33.9 min). The hydrophobicity transfer test showed that fumed silica promoted faster low molecular weight (LMW) migration, while MoS2 and WS2 slowed LMW diffusion due to stronger filler-matrix interactions. Experimental improvements-such as optimized sample preparation, electrolyte control, and voltage ramping-significantly reduced test variability, improving DDT reproducibility. The findings confirm that MoS2 and WS2 nanosheets substantially enhance the long-term hydrophobic stability of SiR composites under HVDC stress, highlighting their suitability for outdoor insulation applications.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
·
IF 6.6
Yinchuan Pan,Shudong Liu,Hao Zhang,Youming Shen,Jing Liu,Jianping Wang,Yanxia Gao,Ning Ma
Tetracyclines (TCs) residues in dairy products pose a potential threat to food safety and public health, necessitating sensitive and selective detection methods. In this study, a fluorescence-based sensing method was constructed by embedding nitrogen and phosphorus co-doped graphene quantum dots (NP-GQDs) into graphite carbon nitride (g-C 3 N 4 ). The composite was subsequently combined with doxycycline-specific molecularly imprinted microspheres (MIM) to obtain MIM@NP-GQDs/g-C 3 N 4 . The composite-based assay exhibited a linear range of 2–50 ng/mL (R 2 > 0.99), with detection limits of 0.8–1.8 ng/mL for three TCs. The method showed excellent reproducibility, allowing up to six reuse cycles, and recovery rates in TC-free dairy samples ranged from 83.5 % to 96.7 % (RSD <5 %). This study demonstrated the application of MIM@NP-GQDs/g-C 3 N 4 for TC detection in dairy products, offering a highly selective and reusable fluorescence-based detection strategy.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Meiijian Wang,Hao Wang,Shiyun Lou,Dexi Tao,Yaolin Tang,Bingfang Zou,Yongqiang Wang
Liquid-phase microextraction (LPME) relies critically on precise solvent control to achieve efficient extractant recovery. To address this, we developed a magnetic microparticle-based dispersive liquid-phase microextraction (MP-DLPME) strategy, employing hydrophobic porous Fe 3 O 4 microparticles (∼1 μm) as solvent-stabilizing microcarriers. These microparticles were functionalized via covalent grafting of 3-chloropropyltriethoxysilane (CPS) and optimized porosity, enabling rigid confinement of organic solvents (e.g., 1-octanol) through dual mechanisms: (I) hydrophobic solvent anchoring to modified surfaces and (II) capillary trapping within mesopores. When applied to hydrophobic polycyclic aromatic hydrocarbons (PAHs) and organophosphorus pesticides (OPPs), the system generates solvent-encapsulated magnetic aggregates that dynamically fragment into microdroplets under vortex-induced shear forces, facilitating efficient analyte extraction via magnetic collection. By integrating the high efficiency of dispersive extraction with the rapid recovery of magnetic separation, the MP-DLPME method provides a robust sample pretreatment solution for environmental water analysis.
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MARINE POLLUTION BULLETIN
·
2025
·
IF 4.9
Ruicong Gao,Jiaqi Lu,Zhixuan Wei,Hao Xu,Siyuan Jing,Minghuo Wu
PAHs, a group of persistent organic pollutants with significant carcinogenic and mutagenic effects, are widely distributed in the environment and can accumulate in sediments, posing potential threats to mangrove ecosystems and human health. This study is one of the most recent studies that presents an analysis of the vertical contamination characteristics of PAHs in mangrove sediments down to a depth of 80 cm, utilizing a combination of solid-phase extraction and gas chromatography–mass spectrometry. The results showed that the mean value of PAHs was 258.82 ng/g (range from 226.41 to 320.07 ng/g) in the studied mangrove areas (Beihai City, Guangxi). The results indicated a decline in PAH concentrations with increasing sediment depth, with values of 259.3 ng/g in surface sediments (0–10 cm), 240.9 ng/g in intermediate sediments (30–40 cm), and 241.1 ng/g in deeper sediments (60–70 cm). This suggests that PAHs not only remain in the surface layer of the sediment, as in previous studies, but also migrate vertically within the sediment. Based on the results, the potential ecological risks of PAHs were also evaluated by the ERL/ERM model. After environmental risk assessment, it was found that among the six PAHs studied, the contents of all except acenaphthene were lower than the ERL, indicating a low ecological risk. This study elucidates the vertical distribution of PAHs in mangrove sediments, offering crucial data for ecological risk assessment and establishing a foundation for future research on PAH transport mechanisms and their ecological impacts, thereby informing environmental management strategies.
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Drug Delivery and Translational Research
·
2019
·Vol. 10
·期 1
·122-135
·
IF 3.111
Lu Xiaoyu,Fang Min,Yang Yue,Dai Yu,Xu Jiaqiu,Zhao Di,Lu Yang,Chen Xijing,Lu Shan,Li Ning
PEGylated triacontanol (mPEG 2k -b-TRIA) was developed as a dual-functional polymer with remarkable biocompatibility. The polymer could self-assemble to micelles with critical micelle concentration (CMC) 17.62 μg mL −1 . Docetaxel-loaded mPEG 2k -b-TRIA micelles (DTX PMs) were fabricated to evaluate the feasibility of mPEG 2k -b-TRIA as drug delivery system. DTX PMs achieved desirable particle size of 93.7 nm, drug loading of 6.66%, and drug encapsulation efficiency of 89.87%. The drug release was based on first-order kinetics model, thus enabling prolonged release. Meanwhile, pharmacokinetic study also revealed that DTX PMs could improve the exposure level of DTX and prolong its systemic circulation time. Furthermore, DTX PMs demonstrated significantly enhanced cytotoxicity and cellular uptake in vitro compared with DTX solution. The in vivo tumor inhibition study carried out on MCF-7 bearing BALB/c mice model also validated that DTX PMs exhibited stronger anti-tumor activity but low toxicity. Notably, mPEG 2k -b-TRIA made some contribution to inhibit the growth of breast cancer cells in vitro and in vivo, indicating the potential as anti-tumor complementary agents. All the results suggested that mPEG 2k -b-TRIA polymer as a vehicle in the formulation of anti-cancer drugs may provide an effective way to improve their therapeutic efficacy. Consequently, the mPEG 2k -b-TRIA polymers would be another promising carrier for hydrophobic anti-cancer drugs delivery.
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JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Jing Li,Li Xu,Zhi-guo Shi
In this study, the ethyl-bridged hybrid silica microsphere with hierarchically meso-macroporous structure was initially synthesized through a method combining dispersion polymerization with sol-gel transition and phase separation. The flow-through macropores rendered the microsphere a rough surface like a waxberry, and thus the material was named as waxberry-like ethyl-bridged hybrid silica sphere (WEHS). WEHS was characteristic of appropriate alkali-stability, which was highly difficult for the pure silica. Additionally, WEHS possessed hierarchical meso- and macropores, which added additional value for faster mass transfer than the conventional fully porous silica materials. Taking the advantages of WEHS, it was successfully applied as the substrate to immobilize lipase; the prepared immobilized lipase exhibited high catalytic activity and favorable reusability under alkaline conditions, which was significant in pitch control of neutral-alkaline papermaking industry. Moreover, as the high-performance liquid chromatographic stationary phase matrix, WEHS made the separation under alkaline mobile phase into a reality for the silica-based materials. Besides, an ultra-fast and efficient separation in minutes was achieved with lower consumption of solvents and saving analytical time, which is highly desired in modern analysis. In general, WEHS was a novel and promising candidate in the myriads of silica-based materials.
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