PHYTOMEDICINE
·
2025
·
IF 8.3
Linlin Ding,Qingjie Li,Kaige Qi,Yonghui Chen,Na Hu,Sangyu Hu,Tianqi Fang,Shuang Guan,Jianfeng Wang,Jiazhang Qiu,Xuming Deng,Lei Xu
Background Salmonella pullorum ( S. pullorum ) is an enteric pathogen that impairs growth performance, leading to substantial economic losses. Evidence demonstrates that the natural flavonoid phloretin can modulate intestinal microbiota functionality, and which underpins its therapeutic efficacy in ameliorating gastrointestinal disorders. However, the protective effects of phloretin against S. pullorum infections and underlying mechanisms remain unelucidated. Purpose This study aimed to elucidate the protective effects and mechanisms of phloretin in improving defense against S. pullorum infection by modulating gut microbiota in chicks. Methods H&E staining, RT-qPCR and ELISA assays were used to assess the protective potentials of phloretin in S. pullorum -infected chicks. Then, 16S rRNA gene sequencing and untargeted metabolomics were employed to identify key microbiota and metabolites regulating the intestinal microenvironment. Moreover, fecal microbiota transplantation (FMT) and dietary metabolite supplementation were conducted to reshape the gut microbiota, elucidate the interaction between the microbiota and S. pullorum infection. Results Phloretin treatment alleviated intestinal injury and enhanced growth performance in S. pullorum -infected chicks via improved intestinal barrier integrity, suppression of inflammatory responses, and restructuring of gut microbial composition. Additionally, these beneficial effects were also observed following FMT from phloretin-treated donors. Subsequent microbial and untargeted metabolomic analysis revealed that phloretin significantly enriched abundance of the functional bacterium Faecalibacterium , and the microbiota-derived phenylalanine metabolites 3-phenylpropionic acid (3-PPA). Importantly, 3-PPA supplementation attenuates S. pullorum -induced intestinal barrier damaged and inflammation in chicks through modulation of the AhR/IL-22/STAT-3 signalling axis. Conclusion These findings provide new insights into the therapeutic potentials of phloretin for S. pullorum -infected chicks.
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 5
·1772-1776
·
IF 3.288
Xin Shu,Yongren Chen,Chunling Yuan,Yilin Wang
A novel and sensitive method for ascorbic acid (AA) determination utilizing Ag+–3,3′,5,5′-tetramethylbenzidine (TMB) as a colorimetric probe was proposed. In a weak acidic medium, Ag+ could oxidize TMB to oxidized TMB (ox-TMB) and cause a blue color solution with an absorption peak centered at 652 nm. However, the addition of AA could induce the reduction of ox-TMB, leading to shallower blueness and lower absorbance. Under the selected experimental conditions, there was a good linear relationship between the decrease of absorbance (ΔA) and AA concentration in the range of 0.5–60 μM, and the detection limit was as low as 0.23 μM. The feasibility of this method was verified in commercial beverages with recoveries of 95.8–104.1%. The method has the advantages of simplicity and practicability. More importantly, no nanomaterials need to be prepared.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 20
·15500-15506
·
IF 3.84
Na Wang,Xiaojing Liang,Qian Li,Yuan Liao,Shijun Shao
A novel solid-phase extraction (SPE) sorbent was synthesized by chemical immobilization of nitro-substituted 3,3′-bis(indolyl)methane onto pure silica. The nitro-substituted 3,3′-bis(indolyl)methane-modified silica was evaluated by elemental analysis (EA) and Fourier transform infrared spectroscopy (FT-IR). Coupled to high performance liquid chromatography (HPLC), the extraction performance of the sorbent was evaluated by using five flavonoids as model analytes. The results showed that the new sorbent could offer multiple intermolecular interactions, such as π–π, hydrophobic, and hydrogen bonding interactions. Several factors including the extraction material, sample solution pH, sample loading rate, eluent type, volume of eluent, elution rate, and volume of sample loading were optimized. Under the optimal conditions, the proposed method was applied for the analysis of five flavonoids in grape juice. Satisfactory linear ranges for flavonoids were obtained in the range of 10–200 ng mL−1 for myricetin, 5–200 ng mL−1 for quercetin and apigenin, and 1–200 ng mL−1 for luteolin and kaempferol, with correlation coefficients (R) ranging from 0.9908 to 0.9996. Limits of detection (LODs) were in the range of 0.5–10 ng mL−1. The recovery values of spiked grape juice ranged from 91.5% to 120.7% with relative standard deviations (RSDs) less than 9.12% (n = 5). As a novel solid-phase extraction sorbent, the nitro-substituted 3,3′-modified silica exhibited a higher extraction efficiency towards the tested compounds than diol-modified silica.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2018
·
IF 3.767
Zhonghua Xue,Lulu Xiong,Honghong Rao,Xiuhui Liu,Xiaoquan Lu
In this work, we proposed a naked-eye colorimetric strategy for simultaneous detection of cysteine and lysine based on 3,3′,5,5′-tetramethylbenzidine (TMB) system in a neutral condition (pH 7.0). Based of the fact that Ag + could oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) and induce a blue color solution corresponding to an absorption peak centered at 652 nm, thus the adding of cysteine or lysine to formation of different product results in the blue color fading to colorless quickly or change to scarlet, respectively. With this strategy, the limit of detection in experimental measurement for cysteine and lysine is 2.46 μM and 3.87 μM, respectively. And the selectivity can also be guaranteed among the other amino acid. The cost is quite low since the use of TMB and Ag + . More importantly, the colorimetric detection operation is very simple without any further modification process and the reaction under physiological conditions is more meaningful for detection of amino acid.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2022
·
IF 4.01
Qamar-un-Nisa Tariq,Yu-fan Bi,Saira Manzoor,Maher-un-Nisa Tariq,Wen-Li Cao,Wen-Shuai Dong,Jian-Guo Zhang
Due to the acidity and the limited applications of 3,4-dinitropyrazole (DNP), two types of nitrogen-rich energetic cocrystals (DD1 and DD2) based on DNP and 3,4-diaminofurazan (DAF) were synthesized. The magnificently grown cocrystals were thoroughly examined by Fourier transform infrared spectroscopy, powder X-ray diffraction, single-crystal X-ray diffraction, elemental analysis, and nuclear magnetic resonance. A differential scanning calorimetry analysis revealed that both cocrystals have different endothermic peaks and remarkably similar exothermic peaks. Moreover, thermogravimetric analysis demonstrates that the thermal decomposition of DD1 and DD2 seems to be three mass loss stages. Hirshfeld surface analysis suggests that O···H (hydrogen bonding) close contacts contribute the most to the stabilization of DD1 and DD2. Subsequently, the pH values of DD1 and DD2 indicate that cocrystals reduce the acidity of DNP; additionally, the inclusion of hydrogen peroxide can improve the oxygen balance of the cocrystal to a certain degree. The obtained cocrystals have high nitrogen contents and acceptable sensitivities to external stimuli (friction and impact) that prove them as promising candidates for expanding the applications of DNP in the field of energetic materials after overcoming an acidity problem.
研究领域:
化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2014
·
IF 2.045
Yu Sun,Yu-Feng Hu,Chao Miao,Zhen-Yu Yang,Chen-Chen Jiang,Xiao-Ming Peng,Yu-Fei Chen
The densities of the binary aqueous solutions of 1-carboxymethyl-3-methylimidazolium chloride ([HOOCMMIm]Cl), 1-carboxymethyl-3-propylimidazolium chloride ([HOOCMPIm]Cl), 1-(2-hydroxylethyl)-3-propylimidazolium chloride ([HOEtPIm]Cl), and the ternary solutions [HOOCMMIm]Cl + [HOOCMPIm]Cl + H2O, [HOEtPIm]Cl + [HOOCMPIm]Cl + H2O, and [HOEtPIm]Cl + [HOOCMMIm]Cl + H2O have been measured at (293.15, 298.15, and 303.15) K, respectively. The densities of the examined ternary solutions have been predicted by the equation of Patwardhan and Kumar established for the density of aqueous electrolyte solutions. The predictions were not very satisfactory probably due to the presence of the functional group −COOH. The coupling of the Patwardhan and Kumar’s equation and the rule of Young and Smith considerably improves the predictions, and the predicted results show excellent agreements with the experimental results.
研究领域:
物理化学
·
化学工程
·
热力学
·
离子液体
·
溶液化学
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Hongrui Jia,Jiahao Mi,Zhen Li,Linghang Wang
Sm-doped Pb(Mg 1/3 Nb 2/3 )-PbTiO 3 (PMN-PT) binary ferroelectric ceramics has received extensive attention due to the excellent dielectric and piezoelectric properties . However, its Curie temperature ( T c ) is greatly reduced by Sm doping and T c is only 89 °C at the optimum piezoelectric coefficient. In this work, Sm-doped Pb(Mg 1/3 Nb 2/3 )O 3 –Pb(Zn 1/3 Nb 2/3 )-PbTiO 3 (Sm: PMN-PZN-PT) ternary ferroelectric ceramics near MPB were successfully manufactured and the crystal structure, microstructure and electric properties were investigated with respect to Sm doping content. A high piezoelectric coefficient of 870 pC/N and a high T c of 146 °C were achieved simultaneously in PMN-PZN-PT ternary ceramics with 1% Sm doping content. In addition, the room temperature dielectric constant was greatly improved by Sm doping and the P r , P s and E c values gradually decrease with the increase of Sm content. These results indicate that Sm-doped PMN-PZN-PT ceramics may be an attractive material for high performance piezoelectric device applications.
研究领域:
材料科学
·
物理学
✓ 已验证
DOI
高影响因子
INORGANIC CHEMISTRY
·
2021
·
IF 5.165
Xuping Shi,Wenbin Zhang,Wenbing Cai,Shujuan Han,Zhihua Yang,Shilie Pan
In virtue of the essential role in controlling polarized light, outstanding ultraviolet (UV) and deep-ultraviolet (DUV) birefringent crystals are imperative in many advanced optical instruments. To design the UV and DUV crystals with large birefringence, we paid more attention to combining the excellent gene, π-conjugated [BO3] unit and metal cations beneficial to blue-shift the cutoff edge; finally, two rare-earth borates Li3La2(BO3)3 and Li1.75Na1.25La2(BO3)3 have been synthesized using the high-temperature solution method. Compared with Na3La2(BO3)3, Li3La2(BO3)3 and Li1.75Na1.25La2(BO3)3 are isostructural, and the isolated [BO3] units are arranged nearly parallel to each other in the structure, which is conducive to generating a larger birefringence. The structural comparison between the two crystals and Na3La2(BO3)3 indicates that the various coordination environments of alkali metal cations play an important role in the evolution of the crystal structure from Li3La2(BO3)3 and Li1.75Na1.25La2(BO3)3 to Na3La2(BO3)3. This work can contribute to a better understanding of the enhancement in birefringence from Na3La2(BO3)3 (0.023 @ 1064 nm) to Li3La2(BO3)3 (0.078 @ 1064 nm) with the perspective of structure–property relationships. Meanwhile, the two title crystals possess the DUV cutoff edge (<190 nm), suggesting that they can be applied as the DUV birefringent crystals.
研究领域:
材料科学
·
光学材料
·
无机化学
·
晶体工程
✓ 已验证
DOI
高影响因子
TURKISH JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 4
·597-619
·
IF 1.3
YUAN HANG,CAO GUI-PING,LV HUI
The reaction solvent and catalyst play an essential role in the Prins reaction for the synthesis of 3-methyl-3-buten-1-ol (MBO) from formaldehyde and isobutene. The reactivity of the solid base catalyzed Prins condensation reaction by formaldehyde and isobutene in supercritical CO2 was investigated using CsH2PO4 modified HZSM-5. We finds that the alkaline sites of the alkali-loaded catalyst can extract the α-H on isobutene to generate olefin carbon negative ions, while the supercritical CO2 with weak Lewis acidity can activate formaldehyde to carbon positive ions, which can combine more easily with the carbon negative isobutene to react, thus improving the reactivity of the reaction system.
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2019
·Vol. 45
·期 8
·11068-11072
·
IF 3.45
Yanqing Lai,Zhen Sun,Liangxing Jiang,Xiaojing Hao,Ming Jia,Li Wang,Fangyang Liu
NASICON-type LiZr 2 (PO 4 ) 3 (LZP) has a high bulk ionic conductivity (∼10 −4 S cm −1 ) and a wide electrochemical stability window between 0 and 5.5 V. However, LZP suffers from a problem in which its desirable rhombohedral phase transforms to the triclinic phase (∼10 −8 S cm −1 ) below 50 °C. In this work, we reported a rhombohedral-structured LiZr 2 (PO 4 ) 3 (α-LZP) at room temperature with a high total Li-ion conductivity (2.8 × 10 −6 S cm −1 ) by a microwave sintering process. Moreover, this approach realized a drastic reduction in the sintering temperature from the typical 1200 °C–900 °C with only a 4 h holding time. Using this approach, we also demonstrated the successful synthesis of Ca-incorporated Li 1.2 Ca 0.1 Zr 1.9 (PO 4 ) 3 (LCZP) with an ionic conductivity of 1.7 × 10 −5 S cm −1 .
研究领域:
材料科学
·
电化学
·
固体离子学
·
储能技术
·
陶瓷工艺
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Li Huang,Shangying Qin,Yuanjin Xu,Shiqi Cheng,Jin Yang,Yilin Wang
MnO 2 nanosheets (MnO 2 NSs) were prepared and used as both oxidant of 3, 3′, 5, 5′- tetramethylbenzidine (TMB) and recognizer of UA, a simple colorimetric approach for sensitive detection of uric acid (UA) was established. In the medium of weak acid, MnO 2 NSs exhibited strong oxidation capacity and could oxidize colorless TMB to generate a blue oxidation product (oxTMB) with an absorbance signal. However, the introduction of UA could inhibit MnO 2 NSs - mediated TMB oxidation and reduce the formation of oxTMB, resulting in the blue fading and the absorbance weakening. Under the conditions of incubation time 20 min, pH 3.0, TMB 0.5 mM, and MnO 2 NSs 50 μM, the decrease in absorbance was linearly dependent on UA concentration in the range of 0.5–30.0 μM with a detection limit of 0.21 μM. Furthermore, the approach has been applied for determining UA in human urine samples with recoveries between 93.1 and 102.4 % and relative standard deviation less than 3.0 %.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Chen Lexian,Lv Qianqian,Cai Jianghong,Liang Jiajie,Liang Ziyan,Lin Jiahui,Xiao Ying,Chen Ruiyao,Zhang Zhiling,Hong Yue,Ji Hong
Coumarin derivatives have diverse structures and show various significant biological activities. Aiming to develop more potent coumarin derivatives for cancer treatment, a series of coumarin acrolein hybrids were designed and synthesized by using molecular hybridization approach, and investigated for their antiproliferative activity against A549, KB, Hela and MCF-7 cancer cells as well as HUVEC and LO2 human normal cells. The results indicated that most of the synthesized compounds displayed remarkable inhibitory activity towards cancer cells but low cytotoxicity on normal cells. Among all the compounds, 5d and 6e were the most promising compounds against different cancer cell lines, especially for A549 and KB cells. The preliminary action mechanism studies suggested that compound 6e, the representative compound, was capable of dose-dependently suppressing migration, invasion and inducing significant apoptosis. Furthermore, the combined results of network pharmacology and validation experiments revealed that compound 6e induced mitochondria dependent apoptosis via the PI3K/AKT-mediated Bcl-2 signaling pathway. In summary, our study indicated compound 6e could inhibit cell proliferation, migration, invasion and promote cell apoptosis through inhibition of PI3K/AKT signaling pathway in human oral epidermoid carcinoma cells. These findings demonstrated the potential of 3-(coumarin-3-yl)-acrolein derivatives as novel anticancer chemotherapeutic candidates, providing ideas for further development of drugs for clinical use.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）