LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Maomao Ma,Junxin Zhao,Zheling Zeng,Dongman Wan,Ping Yu,Ding Cheng,Deming Gong,Shuguang Deng
In this study, the antibacterial activity of monocaprin against Gram-negative foodborne pathogens and its underlying mechanism against E. coli were investigated. It was found that monocaprin exerted excellent antibacterial effects on E. coli , Vibrio parahaemolyticus and Shigella dysenteriae with the minimum inhibitory concentration/minimum bactericidal concentration values of 1.25 mM, 0.625 mM and 0.625 mM, respectively. The results of growth curve and time-kill assay showed that monocaprin could not only delay the growth of E. coli , but quickly inactivate the cells within 15 min. The effects of monocaprin on membrane integrity, inner and outer membrane permeability, proton motive force, and its interaction with model membranes and cell morphology were investigated to explore the antibacterial mechanism of monocaprin. The results indicated that monocaprin rapidly inactivated E. coli by penetrating its outer membrane, then destroying the phospholipid bilayer structure of the inner membrane, as well as dissipating the proton motive force, resulting in increases of the permeability of the outer and inner membranes and leakage of intracellular substances. Besides, monocaprin significantly reduced the viability of E. coli in apple and carrot juices at 4 °C and 25 °C without changing their sensory properties .
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Frontiers in Immunology
·
2025
·
IF 5.9
DongXue Ma,YuXin Zhang,Jia Zhang,Jun Shi,ShanHu Gao,Fei Long,Xin Wang,XingYu Pu,Jiayao Sun,Shuang Liang,Richard D. Cannon,Silas Villas-Boas,Ting-Li Han
Introduction: Escherichia coli Nissle 1917 (EcN) is one of the most extensively studied nonpathogenic Gram-negative probiotic strains worldwide. Recent research has highlighted the ability of EcN outer membrane vesicles (OMVs) to enhance the phagocytosis and proliferation of RAW264.7 macrophages. However, the impact of EcN-OMVs on M1/M2 polarization and metabolic modulation remains unknown.Methods: In this study, we evaluated the metabolic effects of EcN-OMVs on RAW264.7 macrophage polarization using metabolomic, transcriptomic, and fluxomic approaches.Reuslts: We found that the RAW264.7 macrophages phagocytosed EcN-OMVs, triggering upregulation of the HIF-1, mTORC1, and NF-κB signaling pathways. This metabolic reprogramming enhanced glycolysis, suppressed the TCA cycle, elevated intracellular reactive oxygen species (ROS), TNF-α, IL-6, IL-1β, ATP, and nitric oxide (NO) production, and promoted macrophage proliferation, migration, invasion, and M1-type polarization.Discussion: In summary, this research establishes a theoretical foundation for utilizing probiotic OMVs in immunomodulatory therapeutic applications.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Qi Li,Xiao Xiao,Yue Wang,Rui Wang,Qinzhi Wang,Wei Wu
In this work, a versatile conductive bimetallic metal–organic framework combined with aptamer enhanced electrochemical biosensor based on glassy carbon electrode (CMOF/Apt/GCE) was successfully developed for highly sensitive and selective detection of E. coli O157:H7 in animal derived food. The CMOF with large surface area provided an abundance of binding sites for aptamers, which greatly enhancing the selectivity of sensor for E. coli O157:H7. Furthermore, the excellent conductivity of CMOF dramatically improves the electron transfer rate on the modified surface of GCE, contributing to sensitively sensing of E. coli O157:H7. Based on CMOF/Apt/GCE, the E. coli O157:H7 was detected with a low limit of 2.5 CFU·mL −1 and a wide detection range from 10 to 10 7 CFU·mL −1 , superior than those of most previous works. Moreover, it can be applied to pork, chicken, and shrimp samples with good recovery, which indicated its great potential in practical food safety monitoring.
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ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.6
Junchen He,Yuanchan Luo,Hui Wu
3-Hydroxypropionic acid (3-HP) is a versatile platform chemical with broad applications in biodegradable plastics and superabsorbent polymers. Acetate derived from waste biomass and industrial off-gases can serve as a sustainable C2 feedstock for 3-HP production. However, the efficient conversion of acetate to 3-HP remains constrained by insufficient intracellular bicarbonate supply and competition with fatty acid biosynthesis for malonyl-CoA. To address these issues, carboxylation efficiency was enhanced in E. coli by co-expressing carbonic anhydrase (CA) with sodium-dependent bicarbonate transporters (BicA) and supplementation with sodium bicarbonate. Further overexpression of fabF was used to inhibit the initiation of fatty acid biosynthesis, reducing competition for malonyl-CoA. The engineered strain achieved a 3-HP yield of 0.73 g/g in shake flask culture, approaching the theoretical maximum without chemical inhibitors. In a fed-batch bioreactor, the 3-HP titer reached 20.55 g/L with a yield of 0.56 g/g (0.37 mol/mol), corresponding to 74.67% of the theoretical maximum yield. This bioprocess enables efficient valorization of sustainable C2 feedstocks, offering a promising pathway for the industrial production of bio-based platform chemicals.
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Food Innovation and Advances
·
2026
·Vol. 5
·期 2
·185-194
·
IF 5.4
Xiaohuan Huang,Ling Zhang,Tingshu Lu,Jie Li,Mian Wang,Wei Dai,Chengyin Zhou,Jia Xu,Yuangen Wu
Escherichia coli (E. coli) poses a grave threat to food safety, underscoring the need for expeditious and precise detection methodologies. Conventional colorimetric approaches are simple, but they frequently lack accuracy and stability. This study constructed an aptamer-based colorimetric biosensor, leveraging the enzyme-mimicking activity of octahedral Ag2O nanoparticles (NPs), mediated by oligonucleotides, aiming for highly sensitive foodborne E. coli detection. The P12-55 aptamer can be adsorbed onto the octahedral Ag2O NPs surface, thereby significantly enhancing their oxidase-mimicking activity. The aptamer enhances activity by promoting ·O2− generation, and accelerating electron transfer to the 3,3',5,5'-tetramethylbenzidine (TMB) substrate. In the presence of E. coli in the sensing system, the aptamer exhibits a preferential binding affinity for the bacteria, thereby restoring the oxidase-mimicking activity and enabling the transduction of the detection signal. The biosensor exhibited a range of linear detection of 3 × 102–3 × 108 CFU·mL−1, and the limit of detection (LOD) was as low as 4 CFU·mL−1. Recovery rates of 99.1% to 104% were achieved in milk and tap water samples, demonstrating outstanding practicality and reliability. This study proposes a novel and efficient method for the rapid detection of harmful bacteria in food, which is of significant importance for food safety assurance.
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Food Quality and Safety
·
2026
·
IF 4.9
Du Juan,Guo Jiangli,Kang Yifan,Li Yijie,Bai Yanhong
Escherichia coli O157:H7 is a major food safety hazard in beef processing and products, and it can even cause death in severe cases. Immunomagnetic separation (IMS) is an effective sample pretreatment strategy for isolating and enriching target bacteria from food samples, increasing the sensitivity and accuracy of the assay. In this study, an IMS method for E. coli O157:H7, a key safety factor in beef processing and products, was developed, and the interference of the beef matrix was studied. The developed method was highly specific and showed a capture efficiency of 99.98%±1.65% for E. coli O157:H7 in a buffer system, but it decreased significantly in beef samples, which indicated that the matrix has a negative effect on the IMS. By investigating the structure–function relationship between the physicochemical properties of beef and magnetic separation efficiency, sarcoplasmic and myofibrillar proteins were found to be the primary factors affecting IMS efficiency in a dose-dependent manner. Proteins can alter the probe surface, lowering antibody loading and target-binding affinity and ultimately reducing IMS magnetic separation efficiency. Our study provides a theoretical basis for optimizing IMS pre-treatment protocols and enables the development of targeted strategies to mitigate protein-mediated interference, thereby enhancing the sensitivity and reliability of E. coli O157:H7 detection in ground beef.
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Food Bioscience
·
2026
·
IF 6.2
Hongrui Guo,Rui Du,Zijing Dai,Zhidi Xu,Yanqiu Zhu,Xianxiang Wang
Escherichia coli ( E. coli )-induced diarrhea remains a major health challenge in yaks, leading to substantial economic losses in plateau livestock production. Meanwhile, the widespread use of antibiotics has accelerated the emergence of antimicrobial resistance, underscoring the urgent need for green and sustainable antibacterial alternatives. In this study, copper-doped Coptidis Rhizoma -derived carbon dots (Cu-CR-CDs) were successfully synthesized and systematically evaluated for their antibacterial, antioxidant, and intestinal barrier protective effects both in vitro and in vivo . Cu-CR-CDs exhibited a uniform spherical morphology with an average diameter of 3.48 nm, and copper incorporation markedly enhanced its free radical scavenging capacity and conferred with multiple enzyme-like activities, including peroxidase-, superoxide dismutase-, and glutathione peroxidase-like activities. Compared with CR-CDs, Cu-CR-CDs demonstrated significantly enhanced antibacterial activity against E. coli , primarily by disruption of bacterial membrane integrity, increasing permeability, inducing leakage of intracellular biomolecules, inhibiting biofilm formation, and promoting intracellular reactive oxygen species (ROS) generation. In vitro experiments showed that Cu-CR-CDs effectively protected RAW264.7 macrophages and Caco-2 epithelial cells from H 2 O 2 -induced oxidative stress and lipopolysaccharide-induced inflammatory injury through scavenging intracellular ROS, suppressing pro-inflammatory cytokine production, and restoring tight junction protein expression. In vivo , oral administration of Cu-CR-CDs markedly alleviated E. coli –induced diarrhea in mice, reduced intestinal mucosal damage, restored villus structure, mitigated epithelial barrier function, and attenuated systemic inflammation. Moreover, Cu-CR-CDs reshaped gut microbiota composition by decreasing the relative abundance of harmful bacteria (e.g., Proteobacteria, Deferribacteres, and Desulfovibrio ) while increasing beneficial taxa such as Muribaculum, Prevotellaceae, and Akkermansia . Collectively, these results demonstrate that Cu-CR-CDs, as a multifunctional nanozyme with superior performance over CR-CDs, exhibiting potent antibacterial and therapeutic effects against enteric infections, thereby highlighting their potential as a promising nanotherapeutic strategy for the treatment of bacterial diarrhea.
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Foods
·
2025
·Vol. 14
·期 12
·2011
·
IF 5.1
Wei Ma,Haiyun Liang,Keke He,Ting Li,Qiaoni Hui,Yao Zhang,Yuxuan Dong,Yan Jia,Liya Song
Microbial contamination is the leading cause of foodborne diseases and spoilage in food and personal care products. Previous studies by our group have demonstrated that vine tea extract (VTE) and dihydromyricetin (DMY) inhibit the growth ofEscherichia coli. In this study, we further explored the inhibitory mechanisms of VTE and DMY againstE. colithrough a label-free proteomics approach. The proteomic analysis detected 130 and 81 differentially expressed proteins (DEPs) inE.colifollowing VTE and DMY treatment, respectively. The analysis indicated that VTE and DMY inhibit bacterial growth through multiple-target mechanisms. Specifically, they inhibitE. coligrowth by disrupting the cationic antimicrobial peptide resistance pathway, amino acid biosynthesis and metabolism, and nucleotide metabolism. Additionally, VTE disrupts various secondary metabolic pathways, while DMY interferes withE. coliribosome assembly and function, and disrupts cell membrane lipid homeostasis by interfering with fatty acid metabolism. RT-qPCR validation confirmed transcriptional alterations in genes encoding key target proteins. Molecular docking results indicated that DMY may affect bacterial protein synthesis, cationic antimicrobial peptide resistance, and transcriptional regulation by binding to target proteins such as RplB, RplV, LpxA, and YafC. In conclusion, this study systematically deciphered the multi-target inhibitory mechanisms of VTE and DMY againstE. coli, providing a theoretical basis for developing plant-derived antimicrobial agents.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 6
·3503-3514
·
IF 3.612
Ya-Xing Li,Chan Zhong,Hui-Zhen Zhang,Yuan-Yang Zhao,Mei Shu,Guo-Ping Wu
Summary A new gelatin-protocatechuic acid (PCA) film with Escherichia coli O157:H7 phage JN01 was developed and characterised. After incorporated with JN01, swelling value, water vapour permeability, water solubility and elongation at break of gelatin-PCA film were not significantly different. The addition of JN01 increased b value and transparency of film, while it decreased L value, a value and tensile strength of film. Moreover, the gelatin-PCA-JN01 film presented antioxidant activity of 60.07%. Furthermore, JN01 could be steadily released from gelatin-JN01 and gelatin-PCA-JN01 films in aqueous solution, and their release rates were 7.56% and 0.12% after 11 h, respectively. The microstructure analysis showed that JN01 particles were clustered and uniformly distributed in film, and the aggregation would be attenuated in the presence of PCA. Meanwhile, E . coli O157:H7 counts were 1.13 log 10 CFU mL −1 and 0.45 log 10 CFU mL −1 lower in gelatin-JN01 and gelatin-PCA-JN01 films compared with pure gelatin film in vitro at 4 °C for 24 h, respectively. After stored at 4 °C for 7 days, Escherichia coli O157:H7 counts were 1.00 log 10 CFU g −1 and 0.80 log 10 CFU g −1 lower in beef packed with these two gelatin films, compared with beef without gelatin film, respectively. In conclusion, the developed gelatin-PCA-JN01 film has potential application in food preservation.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Shuai Zhao,Tuoyu Zhou,Aman Khan,Zhengjun Chen,Pu Liu,Xiangkai Li
Electrochemical enzyme biosensors based on various modified electrodes have been developed for bisphenol A detection. However, enzyme activity loss and high cost limit their application. In this study, an electrochemical biosensor was developed for bisphenol A detection by displaying tyrosinase on the surface of Escherichia coli cells, followed by adsorption onto a glassy-carbon electrode. The biosensor exhibited a linear relationship between the concentration range of 0.01–100 nM BPA (R 2 = 0.9967) and the current peak. The detection limit was 0.01 nM, which is higher than that of other chemically modified tyrosinase biosensors. For the detection of bisphenol A in tea and juice samples, the fabricated biosensor presented respectable accuracy comparable to that of high-performance liquid chromatography. Furthermore, high recovery rates (97.78%−100.32%) were obtained. Therefore, this biosensor could be a promising and reliable analytical tool for the detection of bisphenol A.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yuanyang Xie,Yu Huang,Jiye Li,Jiangling Wu
A pH-triggered colorimetric assay has been established by using aptamer anchored gold nanoparticles to detect Escherichia coli O157:H7 ( E. coli O157:H7). Gold nanoparticles (AuNPs) functionalized by aptamer via thiol groups were used as probes ( [email protected] ).Solvents of HCl (acid), NaOH (alkaline) and MgCl 2 (nearly neutral) were used as triggers to initiate the movement of [email protected] in the presence of E. coli O157:H7 bacteria respectively. Due to the pH effect of trigger on the surface charges of E. coli O157:H7 and the contribution of aptamer to capture E. coli O157:H7, the movement of [email protected] with the presence of E. coli O157:H7 exhibit dispersion and aggregation respectively, which leads the change of colorimetric signal to an opposite direction. Taking the solvent of NaCl frequently used as trigger for gold nanoparticle based colorimetric sensor into consideration, the utilization of pH dependent triggers provide an alternative for this kind of trigger-based sensor. Design of gold nanoparticle-based colorimetric biosensing assay suiting a wide range of trigger is more flexible for specific diagnosis. These results demonstrate a great potential to design bacterial sensor with high sensitivity to defend public health.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Hongyan Shen,Yingying Liu,Yinan Liu,Zemeng Duan,Pengpeng Wu,Zhifen Lin,Haoyu Sun
Silver antibacterial compounds (SACs) and quorum sensing inhibitors (QSIs), as the potential antibiotic substitutes, have been recommended to prevent and treat microbial infections for the purpose of controlling the increasingly serious bacterial resistance induced by the abuse of antibiotics. However, there is little information regarding the resistance risk of these compounds, especially their mixtures. In this study, bacterial mutation and RP4 plasmid conjugative transfer among bacteria were used to characterize the bacterial endogenous and exogenous resistance, respectively. The effects of SACs (including silver nitrate (AgNO 3 ) and silver nanoparticle (AgNP)), QSIs, and their binary mixtures on the bacterial resistance were investigated via setting the frequency of mutation and conjugative transfer in Escherichia coli ( E. coli ) as the test endpoints. The results indicated that these two endpoints exhibited hormetic dose-responses to each treatment. Furthermore, the joint resistance actions between SACs and QSIs were all judged to be antagonism. Correlation analysis suggested that the promotion of the bacterial resistance in each treatment was closely related to its toxicity. It was speculated that AgNO 3 and AgNP might both release Ag + ions to facilitate the E. coli resistance, while QSIs probably acted on LsrR and SdiA proteins to stimulate the bacterial mutation and accelerate the RP4 plasmid conjugative transfer, respectively. These findings imply that the bacteria may generate targeted stress response to the survival pressure from environmental compounds, displaying hormetic phenomenon in resistance-related test endpoints. This study provides a new insight into the resistance risk induced by SACs and QSIs, benefiting the environmental risk assessment of these compounds from the perspective of bacterial resistance.
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