TALANTA
·
2018
·
IF 4.244
Zi-Han Chen,Xin-Yue Han,Ling-Xue Deng,Zi-Yang Lin,Fang-Ya Mu,Shengqiang Zhang,Guoyue Shi,Min Zhang
Tb 3+ -doped carbon dots (Tb 3+ @CDs) were prepared in a facile hydrothermal method by using ammonium citrate as carbon source and Tb 3+ as dopant. A 15-bp GT-rich single-strand DNA (ssDNA) was introduced to sensitize Tb 3+ via the antenna effect for generating two fluorescence signals (CDs and Tb 3+ ), forming a conjugate of Tb 3+ @CDs/ssDNA. The ratiometric fluorescence of Tb 3+ @CDs/ssDNA could be reversibly regulated by Ag + and Cys, in which the fluorescence peak at 546 nm of Tb 3+ could be switched to “On” or “Off” as the signal indicator while the fluorescence peak at 444 nm of CDs remained constant as the build-in reference. The proposed Ag + /Cys-mediated reversible fluorescence changes in Tb 3+ @CDs/ssDNA was also proven for the design of a self-calibrating ratiometric fluorescence logic system. By integrated with the specific reaction between H 2 O 2 and Cys, Tb 3+ @CDs/ssDNA was applied for ratiometric fluorescence detection of H 2 O 2 . More importantly, the sensing strategy could be further successfully extended to the monitoring of H 2 O 2 -produced oxidase-related reactions, such as GOx-biocatalyzed oxidation of glucose (the limit of detection: 0.06 μM) and was well applied in rat serum compared to commercial kits. This work unveiled a novel ratiometric fluorescent design, which is cost-effective, simple to prepare and easy-to-use without chemical modification or fluorescence labeling .
研究领域:
生物化学
·
分析化学
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 111
·91302-91307
·
IF 3.84
Nan Wang,Zhenwei Han,Hai Fan,Shiyun Ai
In this study, copper nanoparticles modified graphitic carbon nitride nanosheets (Cu NPs/g-C3N4) as a novel peroxidase mimetic were successfully prepared by the calcination of dicyandiamide–Cu2+ complex with the assistance of humic acid. The morphology and structure of the product were characterized by X-ray powder diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The prepared Cu NPs/g-C3N4 was found to have highly peroxidase-like activity, which can rapidly catalyze the oxidation of peroxidase substance 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue color in the presence of H2O2. Accordingly, a simple, selective and fast colorimetric method was developed for H2O2 and glucose detection. The prepared Cu NPs/g-C3N4 exhibited low detection limits of 3.2 × 10−8 M and 3.7 × 10−7 M for H2O2 and glucose, respectively, due to the excellent peroxidase-like activity of Cu NPs/g-C3N4 originating from the synergistic effect of Cu NPs and g-C3N4. In this work, we utilized the easily forming complex ability of dicyandiamide and Cu2+ ions to form a homogeneous precursor solution, and then obtained the CuNPs/g-C3N4 product; such a method may have wide applications in novel composite nanomaterial preparation. The product may have promising applications in medical diagnostics and biotechnology fields.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Tan Jingying,Geng Weifu,Li Junde,Wang Zhen,Zhu Shaohao,Wang Xiuzhong
The mimic enzyme has become a research hotspot in recent years because of its advantages of high stability, convenient preparation, and low price. In this article, Co3O4 nanosheets synthesized by a simple hydrothermal method possess the characteristics of a peroxidase-like activity. The results demonstrated that 3,3′,5,5′-Tetramethylbenzidine (TMB) could be oxidized by H2O2 to produce a typical blue product (oxTMB) which has a strong absorption at 650 nm wavelength with the help of the Co3O4 nanosheets. Thus, a simple and sensitive colorimetric detection method for H2O2 was established with a good linear relationship (2–200 μM) and a low limit of detection (0.4 μM). Meanwhile, the colorimetric product can effectively quench the fluorescence emitted by Ru(bpy)32+. Therefore, a colorimetric and fluorescence dual detection mode photochemical sensor for H2O2 detection is constructed based on the principle of the inner filter effect (IFE) between the colorimetric product (oxTMB) and Ru(bpy)32+. It can effectively avoid the false positive problem of a single detection mode. In the presence of glucose oxidase, glucose can be catalyzed to produce gluconic acid and H2O2; therefore, the sensor can also be used for the determination of glucose with a good linear relationship (0.02–2 μM) and a low limit of detection (5 nM). Experimental results showed that the sensor has a high sensitivity and strong anti-interference ability which can be used for the detection of actual samples.
研究领域:
生物传感器
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Chunqiu Xia,Xiaomin Zhang,Xiaotao Liu,Dehuai Wu,Liangqia Guo
2, 2′-azinobis-3-ethylbenzthiazoline-6-sulfonate (ABTS), 3, 3′, 5, 5′-tetramethylbenzidine (TMB), o-phenylenediamine (OPD) are typical chromogenic substrates to evaluate the activity of peroxidase-like nanozymes. However, these substrates are toxic, unstable, and the coloration reactions are slow. Herein, the classical starch-sodium iodide reagent was utilized as the chromogenic substrate to evaluate peroxidase-like activity of nanozymes. Starch-sodium iodine is less toxic, more stable and faster color reaction than ABTS, TMB and OPD. Under the catalysis of peroxidase-like nanozymes such as Mo 5 N 6 nanosheets , graphene oxide nanosheets, Fe 3 O 4 nanoparticles , CeO 2 nanoparticles, iodide ion (I – ) is oxidized by H 2 O 2 to produce blue color complex (starch-I 2 ). Taking Mo 5 N 6 nanosheets as the peroxidase-like nanozyme, the color reaction completed within 5 min at room temperature (25 ºC), and the chromogenic substrate remained stable within 30 days. The maximum reaction rate (V max ) using starch-sodium iodine as the chromogenic substrate was more than 100-fold higher than that of TMB. Based on this, a sensitive colorimetric sensor and facile portable agarose gel test kits were proposed and applied for the detection of H 2 O 2 residue in liquid milk samples with fast response.
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jingjing Hu,Wei Cheng,Yishuo Zhao,Yian Song,Huan Xu,Hao Zhou,Huifang Yang,Jie Sun,Ruan Chi
Recently, bi-functional catalysts have emerged as an effective approach for enhancing the performance of electro-Fenton in degrading antibiotics by simultaneously improving H 2 O 2 generation and Fe circulation. Herein, we prepared a SO 4 2- modified Ce x Zr 1-x O 2 bi-functional catalyst to construct an efficient e-Fenton system for degrading norfloxacin. Results display that the fabricated catalyst exhibited superior performance in degrading norfloxacin owing to durative generated ⋅OH, which was proved to be the dominant active species. The H 2 O 2 generation capacity of that catalyst was twice higher than the pure e-Fenton and its faradaic efficiency even came up to 75%. The amount of soluble total Fe and Fe 2+ ions of that catalyst were both increased twofold compared to the blank system, declaring the acceleration of Fe recycling process. Characterizations and density function theory calculations proposed a synergistic mechanism that attributed the simultaneously improvement in H 2 O 2 generation and Fe 2+ circulation to the multiple regulated interface properties, the promoted 2e - ORR activity caused by enriched oxygen vacancies at Ce sites, and the accelerated electrons transfer owing to the strengthened acidic sites for the coordination between Zr sites and S species. Furthermore, that catalyst is also proved to be feasible, well-recycled and practical for application in wastewater purification.
研究领域:
环境科学
·
催化材料
·
废水处理技术
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2023
·
IF 4.6
Bin Wu,Xiangyun Wang,Bo Yang,Yunjian Ma,Frank Hollmann,Yonghua Wang
In the present work we propose a bienzymatic cascade for the oxyfunctionalisation of cycloalkanes to cyclic alcohols/cyclic ketones. By combining a H 2 O 2 -dependent peroxygenase with a O 2 -consuming and H 2 O 2 -producing alcohol oxidase an overall aerobic oxidation system was established. A convincing proof-of-concept is presented and some current limitations are outlined.
研究领域:
生物化学
·
酶工程
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Shujun Sun,Jun Zhang,Changdong Sheng,Hui Zhong
NO in flue gas is one of the atmospheric pollutants, and it is necessary to study effective NO removal technology. It was identified that the alkaline H 2 O 2 system achieved over 98 % NO removal efficiency with a gas–liquid contact time of 3 s in this paper. This paper investigates the mechanism of NO removal in alkaline H 2 O 2 solution through both experimental and theoretical calculations. The results reveal the NO removal process can be divided into three phases by the NaOH/H 2 O 2 molar ratio. (1) The ∙O 2 – dominated phase, where trace metal Fe catalyzes ∙O 2 – radical generation from H 2 O 2 as Fe (OH) 2+ , reacting primarily with NO to form ONOO – . The energy barrier for this process is1.299 kcal/mol, with a reaction rate constant of 9.12 E + 11 s -1 M −1 . (2) The HO 2 – dominated phase, HO 2 – was generated through OH – and H 2 O 2 neutralization, which converts NO to NO 2 and regenerating OH – . The energy barrier for HO 2 – reacting with NO is 3.833 kcal/mol, and the reaction rate constant is 2.07E + 10 s -1 M −1 . (3) The O 2 2– dominated phase, O 2 2– was a further ionization of HO 2 – and converting NO to NO 2 – directly. The energy barrier for this process is 7.079 kcal/mol, and the reaction rate constant is 1.49 E + 08 s -1 M −1 .
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 1
·016502
·
IF 3.9
Chenzhong Yao,Huimin Ren,Bohui Wei,Chunxi Yang,Li Ma
WO3@ZnO core–shell nanoarrays with a Z-scheme heterojunction structure have been fabricated by combining electrodeposition and dropping process and used as photoanodes for the production of hydrogen peroxide (H2O2). The photocurrent density of the WO3@ZnO nanoarray photoanode is 1.57 mAcm−2 at the applied potential of 1.8 V vs reversible hydrogen electrode, which exhibits almost 4 time larger than that of pure ZnO nanoarray photoanode. A high photoconversion efficiency to the production of H2O2 (0.53%) and Faradic efficiency (33%) are also reached by this WO3@ZnO nanoarray photoanode under Air-Mass 1.5 Global (AM 1.5 G, 100 mWcm−2). This work provides an effective approach for the application of Z-scheme heterojunction in the photoelectrochemical synthesis of H2O2.
研究领域:
材料科学
·
能源科学
·
环境科学
✓ 已验证
DOI
高影响因子
LUMINESCENCE
·
2012
·Vol. 28
·期 3
·259-264
·
IF 1.731
Zhonghui Wang,Hongjie Song,Huihui Zhao,Yi Lv
Electrogenerated chemiluminescence (ECL) of thiol-capped CdTe quantum dots (QDs) in aqueous solution was greatly enhanced by PDDA-protected graphene (P-GR) film that were used for the sensitive detection of H 2 O 2 . When the potential was cycled between 0 and −2.3 V, two ECL peaks were observed at −1.1 (ECL-1) and −1.4 V (ECL-2) in pH 11.0, 0.1 M phosphate buffer solution (PBS), respectively. The electron-transfer reaction between individual electrochemically-reduced CdTe nanocrystal species and oxidant coreactants (H 2 O 2 or reduced dissolved oxygen) led to the production of ECL-1. While mass nanocrystals packed densely in the film were reduced electrochemically, assembly of reduced nanocrystal species reacted with coreactants to produce an ECL-2 signal. ECL-1 showed higher sensitivity for the detection of H 2 O 2 concentrations than that of ECL-2. Further, P-GR film not only enhanced ECL intensity of CdTe QDs but also decreased its onset potential. Thus, a novel CdTe QDs ECL sensor was developed for sensing H 2 O 2 . Light intensity was linearly proportional to the concentration of H 2 O 2 between 1.0 × 10 −5 and 2.0 x 10 -7 mol L −1 with a detection limit of 9.8 x 10 −8 mol L −1 . The P-GR thin-film modified glassy carbon electrode (GCE) displayed acceptable reproducibility and long-term stability. Copyright © 2012 John Wiley & Sons, Ltd.
研究领域:
分析化学
·
生物传感
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-8
·
IF 15.7
Hu Yunhao,Yang Weifeng,Ma Yuji,Qiu Yong,Wei Wei,Wu Bo,Li Kerui,Li Yaogang,Zhang Qinghong,Xiao Ru,Hou Chengyi,Wang Hongzhi
Solid-liquid contact electrification is a widespread interface phenomenon in nature. Recent research and theory demonstrate that electron transfer during this process holds the potential to initiate interfacial chemical reactions. Here, we design a dual-functional device for generation of H 2 O 2 and energy. Interfacial chemical reactions and solid-liquid contact charging occur simultaneously during the liquid phase flow process. Specifically, electron transfer at the solid-liquid interface induces the formation of hydroxyl radicals (·OH) in the liquid phase, leading to spontaneous generation of H 2 O 2 . The transfer of charges at the solid-liquid interface is accompanied by energy transfer. By designing an external electrode structure, we can effectively harvest the energy from the flowing liquid phase, yielding an output power of up to 5.8 kW/m 3 for water.
研究领域:
材料科学
·
化学工程
·
能源科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Wang Lv,Shangkai Qiu,Lu Xiong,Zhiyong Yan,Hou Wang,Yan Wu
Mo 4d-S 3p orbital hybridization effectively reshapes the electronic band structure. • Sulfur vacancies shift from recombination centers to efficient charge-transfer channels. • Mo S Ni bridging orbital channels establish a robust built-in electric field. • Mo–Ni orbital synergy enables a highly selective 2e⁻ oxygen reduction pathway. This work addresses the fundamental dilemma between charge separation efficiency and surface reaction selectivity in heterogeneous photocatalysis through synergistic orbital engineering. By introducing Mo dopants and sulfur vacancies (V S ) into Zn 3 In 2 S 6 , the localized electronic states of isolated V S are transformed into delocalized charge channels via strong Mo 4d-S 3p orbital hybridization. This orbital-level modulation effectively suppresses charge recombination while optimizing the band structure for visible-light absorption. Coupled with in-situ grown Ni 4 S 3 , an interfacial Mo-S-Ni orbital bridge is constructed, which not only establishes a robust built-in electric field to direct electron flow toward Ni 4 S 3 but also tailors the local coordination environment for selective two-electron oxygen reduction. The resulting heterojunction photocatalyst achieves an exceptional H 2 O 2 production rate of 163.0 ± 2.1 μM h −1 in pure water without any sacrificial agent. Notably, the H 2 O 2 production rate of 252.1 ± 2.4 μM h −1 is attained at a tetracycline hydrochloride (TC) concentration of 20 mg L −1 , 1.5 times higher than that in pure water. This study demonstrates that precise orbital coupling can integrate carrier dynamics management with reaction pathway control, providing a new design paradigm for noble metal-free, high-performance and multifunctional photocatalytic systems. Download: Download high-res image (216KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Green Chemical Engineering
·
2026
·
IF 11.9
Chuan-Bin Li,Ling-Yu Dong,Yi-Fan Wang,Guang-Ping Hao
Electrosynthesis of hydrogen peroxide (eH 2 O 2 ) offers a facile and environmentally benign synthesis alternative through selective two-electron oxygen reduction reaction (2e – ORR). Carbon-based nonprecious electrocatalysts are promising for 2e – ORR, however, its efficiency is often limited by low intrinsic activity and limited accessibility of catalytic sites. Here, we present a family of carbonyl rich carbon spheres with surface wrinkle structure, which was engineered to boost the activity for 2e – ORR and to enlarge its electrochemical active surface area. As compared to common O-doped carbon spheres, our materials with controlled surface wrinkles delivered a 10-fold enhancement of accessible active surface area. Extensive catalytic tests together with characterizations indicate carbonyl moieties are the predominant active sites for O 2 activation. In 0.1 M KOH electrolyte, the catalyst delivered the H 2 O 2 selectivity of > 97.5% over a large potential range from 0-0.67 V vs . RHE, which resulted in a H 2 O 2 production rate of 385 mmol/(g·h) at 0 V vs. RHE. This study provides a useful surface structure fine modulation method for the development of heteroatom-doped carbon electrocatalysts for selective H 2 O 2 synthesis.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）