FOOD CHEMISTRY
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2017
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IF 4.529
Ting Hu,Qilin Huang,Kahing Wong,Hong Yang,Jingsi Gan,Yanru Li
An alkali-soluble polysaccharide was extracted from Lignosus rhinocerotis sclerotia (LRP). Its structural characteristics were determined by GC–MS, FT-IR, GC, 1D and 2D NMR combined with Smith degradation and methylation analysis. The LRP had a (1 → 3)-β- d -Glcp backbone with every three residues bearing a (1 → 6)-linked and hyperbranched side chain that contained three (1 → 6)-β- d -Glcp residues as secondary main chain and two terminal β- d -Glcp residues linked at O3. The degree of branching was 0.76 from GC–MS analysis, implying a highly branched structure for LRP. The M w , < S 2 > z 1/2 , R h and [ η ] values of LRP in 0.25 M LiCl/DMSO were measured by SEC-MALLS-Vis-RI combination technology to be 2.88 × 10 5 g/mol, 30.36 nm, 22.34 nm and 131.50 ml/g, respectively. Furthermore, the exponent α of [ η ] –M w , β of < S 2 > z 1/2 –M w , the fractal dimension d f and molecular parameter ρ were determined to be 0.20, 0.33, 2.50 and 1.36, demonstrating that the LRP was a hyperbranched polysaccharide and adopted a compact coil conformation in LiCl/DMSO.
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JOURNAL OF PHYSICAL CHEMISTRY B
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2013
·
IF 3.607
Jingjing Li,Kaiqi Fan,Libo Niu,Yuanchao Li,Jian Song
The effect of salt on the gelatinization of 2,4-(3,4-dichlorobenzylidene)-d-sorbitol (DCBS), a novel low-molecular-weight gelator, was studied. DCBS showed pronounced hydrogelation and the electron micrographs indicated that the hydrogels consists of globular aggregates. Addition of NaCl to the aqueous medium accelerated the gelation process and also caused the gel’s morphology to change from globular to long fibers. In addition, the thermal properties of the hydrogels were improved with the addition of NaCl. UV–vis and fluorescence emission spectra showed that extensive aggregation of the phenyl rings was responsible for the gelation. The presence of NaCl induced a red shift in the emission peaks of DCBS and a decrease of the pyrene polarity index I1/I3 in the gels, which indicated that there was more π–π stacking in the hydrogels with NaCl than in the gels without NaCl. Variable-temperature 1H NMR spectra further demonstrated that the π–π interactions were enhanced by NaCl. FTIR studies showed that hydrogen bonding was also a contributing factor in the gelation process. Wide-angle X-ray diffraction (WXRD) showed that the hydrogels had a layered structure which did not change with the addition of NaCl. Density functional theory (DFT) calculations indicated the possible molecular packing of the gelator in the nanofibers.
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Bioengineered
·
2017
·
IF 1.691
Wen-Bin Zhou,Jin-Song Gong,Hai-Juan Hou,Heng Li,Zhen-Ming Lu,Hong-Yu Xu,Zheng-Hong Xu,Jin-Song Shi
Phosphatidylserine (PS) is useful as the additive in industries for memory improvement, mood enhancement and drug delivery. Conventionally, PS was extracted from soybeans, vegetable oils, egg yolk, and biomass; however, their low availability and high extraction cost were limiting factors. Phospholipase D (PLD) is a promising tool for enzymatic synthesis of PS due to its transphosphatidylation activity. In this contribution, a new and uncharacterized PLD was first obtained from GenBank database via genome mining strategy. The open reading frame consisted of 1614 bp and potentially encoded a protein of 538-amino-acid with a theoretical molecular mass of 60 kDa. The gene was successfully cloned and expressed in Escherichia coli. Its enzymatic properties were experimentally characterized. The temperature and pH optima of PLD were determined to be 60°C and 7.5, respectively. Its hydrolytic activity was improved by addition of Ca2+ at 5 mM as compared with the control. The enzyme displayed suitable transphosphatidylation activity and PS could be synthesized with L-serine and soybean lecithin as substrates under the catalysis of PLD. This PLD enzyme might be a potential candidate for industrial applications in PS production. To the best of our knowledge, this is the first report on genome mining of PLDs from GenBank database.
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Discover Oncology
·
2024
·Vol. 15
·期 1
·1-11
·
IF 2.8
Jiang Xinru,Li Hongdan,Liu Yang
Objective Hepatocellular carcinoma (HCC) is one cancer with high death rates. Nowadays, there are no effective drugs to treat it. Cyclovirobuxine D (CVB-D) is the primary ingredient of the traditional Chinese medicine (TCM) Buxus microphylla . Here, we try to explore the impacts of CVB-D on human HCC cells and explain the potential mechanisms. Methods HepG2 and Huh-7 cells were used for our experiments. The cell viability and half inhibitory concentration (IC50) were detected by MTT assays. The apoptosis ratio was examined by Annexin V-FITC/7AAD staining and flow cytometry (FCM). The Fe 2+ content was examined by ferrous ion content assays. The malondialdehyde (MDA) content was evaluated by lipid peroxidation MDA assays. The reactive oxygen species (ROS) level was examined by the DCFH-DA probe. The expression of apoptotic markers (Bax and Bcl-2) and ferroptosis-related proteins (GPX4 and FSP1) was detected by western blotting. The in vivo curative effect of CVB was explored using xenograft models established in C-NKG mice. Results The cell viability could be inhibited by CVB-D in HepG2 and Huh-7 cells. The IC50 value of CVB-D on HepG2 and Huh-7 cells are 91.19 and 96.29 µM at 48 h, and 65.60 and 72.80 µM at 72 h. FCM showed that the apoptosis rate was increased by CVB-D in HepG2 and Huh-7 cells. Next, ferrous ion content assays showed that the level of Fe 2+ was increased by CVB-D in HepG2 and Huh-7 cells. Then, we found the level of MDA and ROS was increased by CVB-D. And the Fe 2+ promotion by CVB-D could be reversed by Fer-1. Additionally, western blotting assays showed that the expression of GPX4 and FSP1 was inhibited by CVB-D in HepG2 and Huh-7 cells. Moreover, in vivo, CVB-D displayed excellent anticancer effects in HCC tumor-bearing C-NKG mice. Conclusion CVB-D suppresses the growth in HCC cells through ferroptosis.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
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2024
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IF 3.3
Yang Li,Mijia Li,Shiyi Duan,Sijie Zhang,Huiqiang Lu,Xinchun Guo,Keyuan Zhong
d -Tetramethrin is one of the main components of mosquito control products, and is widely used for the control of dengue fever and insecticide production. Due to its widespread use, d -tetramethrin is a ubiquitous environmental pollutant and poses potential risks to human health. However, the effects of d -tetramethrin on liver morphology and function are not clearly established. In this study, we used zebrafish as an animal model to analyze the acute and chronic effects of d -tetramethrin exposure on the liver. We exposed zebrafish larvae and adults to different concentrations of d -tetramethrin and examined the impact of d -tetramethrin on lipid and glycogen metabolism, cellular properties, oxidative stress , cell proliferation , and apoptosis in the liver. We also analyzed transcriptional changes in genes related to apoptosis, inflammation, and cell proliferation using qPCR . Zebrafish exposed to d -tetramethrin exhibited severe liver damage, as evidenced by the presence of vacuoles and nuclear distortion in liver cells. The liver area in zebrafish larvae of the treatment group was significantly smaller than that of the control group. Significant lipid accumulation and decreased glycogen levels were observed in the livers of both zebrafish larvae and adults exposed to d -tetramethrin. Furthermore, d -tetramethrin exposure induced apoptosis and inflammation in zebrafish embryos. Additionally, d -tetramethrin caused liver damage, metabolic dysfunction, and impaired liver function. These results suggest that d -tetramethrin induces liver toxicity in zebrafish, by inducing oxidative stress and inhibiting cell proliferation.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jing Sun,Zaixu Wang,Yongjin Dai,Moran Zhang,Xinyi Pang,Xiangfei Li,Yingjian Lu
Molds and mycotoxins pose severe threats to health. Bacillomycin D (BD) can effectively inhibit mold growth. Attapulgite (ATP) can provide a good carrier for antimicrobial agents. Natural ATP was acid-modified to obtain H-ATP. It was used to load BD to obtain a novel composite material (H-ATP-BD). The results showed H-ATP had better adsorption performance than ATP. BD was adsorbed up to 93.13 % by adding 30 mg H-ATP and stirring at 40 ℃ for 120 min. Fourier transform infrared spectra (FTIR), size and zeta potential, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) results confirmed successful loading of BD onto H-ATP. The composite showed good inhibition of Aspergillus and adding 0.6 % H-ATP-BD composite was effective in removing 89.06 % of aflatoxin B 1 (AFB 1 ) at 50 °C. Model fitting indicated that AFB 1 removal was a spontaneous exothermic reaction. This research will lay the foundation for the development of efficient and green antimicrobial and toxin-reducing materials.
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JOURNAL OF CHEMICAL PHYSICS
·
2024
·Vol. 160
·期 15
·
IF 3.1
Zhao Miao Miao,Du Na,Zhu Yu Ying,Yen Fei
Many solid crystals exhibit a structural phase transition where a subset of its ions or entire molecules become orientationally ordered. As to why such ordering occurs remains mostly unresolved. We consider the extremely weak magnetic elements arising from the reorientations of the molecules experiencing mutual resonance to play the chief role. Two new features are identified in d -camphor: (1) the magnetic susceptibility abruptly changes when crossing the order–disorder phase transition at T II–III = 239.8 K during cooling and at T III–II = 245.2 K during warming and (2) the complex dielectric constant exhibits two successive discontinuities only 0.2 K apart near the critical temperatures when the sweeping rate is only 0.1 K/min. We discuss how the change in entropy associated with order–disorder transitions in plastic crystals represents temporal changes rather than spatial changes in the system. Our findings may be extended to study why many other crystalline solids exhibit orientational ordering and irreversibility.
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Polymer-Plastics Technology and Materials
·
2024
·
IF 2.6
Jingwen Lan,Rukuan Liu,Sisi Liu,Airong Xu
Eco-friendly silk fibroin/poly(D,L-lactide-co-glycolide) (SF/PLGA) materials were successfully fabricated using a facile strategy. The materials were characterized by scanning electron micrograph (SEM), X-ray diffraction (XRD) and infrared spectra (IR). Systematic investigations were completed to examine the degradation rates in natural soil, cytocompatibility and thermostability of the materials. It is interesting to find that after SF was hybridized by PLGA, the thermostability and degradation rate increased. Meanwhile, the materials show good cytocompatibility. SEM, IR and XRD results reveal that there is hardly any interaction between SF and PLGA in the SF/PLGA material, and SF is physically mixed with PLGA. This study opens up a new horizon in the design and preparation of SF-based materials for promising applications in medical and biodegradable material fields.
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ACS Nano
·
2025
·
IF 16
Juexin Huang,Jingyi Xia,Sravan Baddi,Yu Zhao,Chuan-liang Feng
Piezoelectric biomaterials hold immense potential for next-generation microdevices, yet their piezoelectric performance often trails behind that of conventional synthetic counterparts. This study demonstrates a dual-scale polarization strategy to significantly enhance the piezoelectric response of d-phenylalanine derivatives (d-PheAD). Molecular-level polarization is amplified through ionic interactions with l-glutamic acid-modified Fe3O4 magnetic nanoparticles, while macroscopic alignment of d-PheAD nanoarrays under magnetic fields optimizes collective dipole orientation. This synergistic approach elevates the effective piezoelectric coefficient (deff) from 10.4 to 121.9 pm V–1, outperforming most biomaterial-based piezoelectric systems and many inorganic piezoelectric systems. Integrating these engineered assemblies into a triboelectric-enhanced piezoelectric nanogenerator yields unprecedented electrical outputs (2853 nA, 51.9 V), a 25-fold improvement over baseline configurations. The device achieves real-time detection of trace HCl gas at low concentrations, demonstrating superior sensitivity compared to commercial electrochemical sensors. By bridging molecular design and macroscopic engineering, this work establishes a scalable paradigm for deploying eco-friendly piezoelectric biomaterials in high-performance microelectronics and environmental monitoring technologies.
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Applied Sciences-Basel
·
2019
·Vol. 9
·期 18
·3847
·
IF 2.217
Mingtang Tan,Peiyun Li,Wenhui Yu,Jinfeng Wang,Jing Xie
This study aimed to investigate the effects of glazing with sodium polyacrylate (SP) and D-sodium erythorbate (DSE) on the quality changes of squid during frozen storage. Frozen squid samples were randomly divided into seven groups: (1) CK (unglazed); (2) WG (distilled water-glazed); (3) SG (0.1% SP -glazed); (4) SG-1DSE (0.1% SP with 0.1% DSE -glazed); (5) SG-3DSE (0.1% SP with 0.3% DSE-glazed); (6) SG-5DSE (0.1% SP with 0.5% DSE-glazed); (7) WG-1DSE (0.1% DSE-glazed). The efficacy of the different coatings was evaluated using various indicators, such as water holding capacity (WHC), pH value, low field nuclear magnetic resonance (LF-NMR), color, malondialdehyde (MDA) content value, free amino acids (FAAs) content, intrinsic fluorescence intensity (IFI) and the total sulfhydryl content (SH) content. Intrinsic fluorescence intensity (IFI) and low field nuclear magnetic resonance (LF-NMR) were used as fast monitoring techniques to monitor changes in quality of squid samples. The results showed that compared with the CK and WG groups, coating with either SG or DSE alone resulted in reduced rate of moisture loss (p< 0.05), lipid oxidation (p< 0.05) protein degradation (p< 0.05) and prolonged its shelf-life. The combination of glazing treatment with SG and DSE (groups SG-1DSE, SG-3DSE and SG-5DSE) further improved the protective effects of coating, particularly in the SG-3DSE group. Therefore, the glazing of SG-3DSE is recommended to be used to control the quality of frozen squid and to prolong its shelf-life during frozen storage.Keywords:squid;glazing;frozen storage;sodium polyacrylate;D-sodium erythorbate;quality
研究领域:
食品科学
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海洋生物学
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保鲜技术
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JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
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2020
·Vol. 31
·期 6
·1216-1224
·
IF 1.399
Han Qing,Wei Lanlan,Deng Guozhe,Sun Ying,Deng Jianjun,Tang Zigang,Fan Jinhui,Wang Xia,Kang Xuejun
The detection of vitamin D in complex samples is challenging because of the instability of vitamin D in sample preparation. To resolve this problem, packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D2 (D2) and vitamin D3 (D3) in dairy products. Based on PFSPE, vitamin D could avoid oxidation because of the shorter extraction time. The adsorbent materials, polystyrene/polypyrrole (PS/PPy) nanofibers, were prepared by electrospinning of PS and in situ polymerization of PPy on the surface of PS nanofibers. The linearity was within the range of 1-2000 ng mL−1 in dairy solution, and the recoveries were 91.9-109.1% with a relative standard deviation (RSD) < 13.6%. Therefore, the prepared method could be used for the detection of D2 and D3 in dairy products.
研究领域:
分析化学
·
食品科学
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INTERNATIONAL JOURNAL OF THERMOPHYSICS
·
2020
·Vol. 41
·期 5
·1-12
·
IF 0.794
Mo Songping,He Lijuan,Jia Lisi,Chen Ying,Cheng Zhengdong
Thermophysical properties are important parameters that influence the performance of phase change materials (PCMs) and heat transfer fluids. Micro/nanoencapsulation is an effective technique for preventing leakage and enhancing thermophysical properties of PCMs. In the present study, novel d -mannitol nanocapsules with inorganic shells were synthesized as a medium-temperature PCM. The nanocapsulation was confirmed by scanning electronic microscopy. The specific heat and thermal diffusivity of the nanocapsules and their suspension with heat transfer oil as base fluid were measured by a differential scanning calorimeter and light flash apparatus. The thermal conductivity of the nanocapsule suspension was measured using a hot-disk thermal constants analyzer. The viscosity of the nanocapsule suspension was measured by a rotational rheometer. The results show that the thermal conductivity and thermal diffusivity of the nanocapsules were enhanced compared to the bulk of the PCM due to the inorganic shell. The specific heat, thermal conductivity, and viscosity of the nanocapsule suspension were increased compared to the base fluid. The potential of the nanocapsule suspension as a heat storage and transfer fluid was evaluated.
研究领域:
材料科学
·
热力学
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