CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Wenbin Li,Gaocan Qi,Haihui Ding,Weibin Yan,Xijun Liu,Zhihao Yuan
The safe use of fuel gases in industrial production and daily life requires prompt and accurate detection of their concentrations in the environment. However, due to the strong reducing properties of the flammable gases used as fuel, traditional chemoresistive gas sensors face challenges in simultaneously detecting multiple flammable gases. To address this issue, we have developed a metal oxide-based electronic nose capable of detecting common fuel gases, including CH 4 , H 2 , and CO. By controlling the composition and structure of metal oxides, as well as introducing noble metal modifications, we optimized the response differences of these gas sensitive materials to CH 4 , H 2 , and CO. Four sensitive materials were selected from 8 candidates using principal component analysis to construct gas sensor array on a compact four-channel circuit board. The response sensitivities from the sensor array to various concentrations of CH 4 , H 2 , CO, and their mixtures were utilized to train a backpropagation artificial neural network. A new component-identification outlet layer was integrated with the traditional concentration-recognition to improve the training speed and enable simultaneous identification of gas components and concentrations. Through combined material optimization and dual-output training, the backpropagation artificial neural network achieved 90% training accuracy within just 50 iterations, with both sparse cross-entropy loss and mean squared error reduced below 0.005 after 300 training epochs. The resulting E -nose can accurately identify gas components and determine their concentrations in single-, binary-, and ternary-component mixtures of CH 4 , H 2 , and CO, with detection accuracies exceeding 91%. This study provides a practical approach for E -nose construction, enabling reliable monitoring of flammable gases.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Yibin Yang,Rui Chen,Zhiqiang Zhang,Yuke Qin,Attiqa Parveen,He Bai,Xiaoqiang Jia
Phenanthrene, a pervasive polycyclic aromatic hydrocarbon (PAH), poses significant environmental and health risks due to its persistence and bioaccumulation. This study presents the development of a synthetic bacterial consortium comprising three engineered Escherichia coli BL21(DE3) strains, each harboring distinct catabolic modules encoded by pET-28(a) plasmids for the complete mineralization of phenanthrene. The consortium was engineered by integrating 17 genes critical for phenanthrene degradation, organized into three distinct modules that harness the collective metabolic potential of separate strains to enhance the degradation process. Each module was tailored to a specific phase of phenanthrene catabolism, ensuring the coordinated and efficient breakdown of the pollutant. Following the identification of rate-limiting enzymes within the up- and downstream degradation modules, the eight corresponding genes were subjected to laboratory evolution using the SCRaMbLE method to bolster the degradation capabilities of the strains. The consortium was then tested in a minimal salt medium (MSM) containing 100 mg/L phenanthrene. Following their evolution, the two SCRaMbLE strains E. coli SCRPHE033 and E. coli SCRKA065 exhibited enhanced degradation rates of 11.5 and 25.18 % for their respective substrates. Finally, the comprehensive phenanthrene degradation system, which includes the two SCRaMbLE strains and E. coli CRSA, was subjected to comprehensive optimization, after which it achieved a significant degradation efficiency of 84.33 % for 100 mg/L phenanthrene within a 120-hour timeframe. This research demonstrates the potential of synthetic biology in constructing microbial consortia for environmental bioremediation based on the SCRaMbLE method, offering a promising strategy for mitigating PAH pollution.
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Materials Today Bio
·
2025
·
IF 10.2
Juan Duan,Muxi Chen,Mengyan Wang,Rui He,Ke Li,Lei Shi
Radiation-induced oral mucositis (RIOM) substantially compromises the efficacy of cancer treatment and patient survival, while current therapeutic approaches exhibit limited efficacy in addressing its multifactorial pathogenesis. Curcumin possesses multi-targeted pharmacological properties relevant to RIOM, including antioxidative, anti-inflammatory, and mucosal repair effects, rendering it an ideal therapeutic agent. This study developed a curcumin-based intelligent-responsive integrated therapeutic platform (Cur-VE-Zn@gel) via a hierarchical assembly strategy. Specifically, curcumin was covalently bound to vitamin E to form the active center, while its coordination with Zn 2+ facilitates self-assembly into stable polymeric structures. This design not only significantly enhanced the biosecurity of curcumin but also amplified its antioxidant, anti-inflammatory, and tissue repair capabilities, while exhibited stable DNA repair characteristics, thus establishing a comprehensive radioprotective barrier. This bioactive complex was further incorporated into an adhesive thermosensitive hydrogel consisting of chitosan and poloxamer, which enabled targeted delivery to oral mucosa and bypassed the low systemic bioavailability of curcumin. Comprehensive in vitro and in vivo evaluations demonstrated that the Cur-VE-Zn@gel platform effectively modulated DNA damage repair, oxidative stress, inflammatory cascades, and epithelial regeneration, thereby achieving synergistic multi-targeted therapy for the suppression of RIOM. Ultimately, this biomaterial-based strategy successfully addresses the key pharmacological limitations of curcumin through structural modification and targeted delivery, providing a highly promising, safe, and effective approach for the clinically challenging management of RIOM.
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Small
·
2025
·
IF 12.1
Mianyi Xiang,Yamin Liu,Ziyang Yang,Jinlei Jiang,Weicheng Wang,Yao Hu,Daxiang Cui,Qichao Li
Electronic noses (e-noses) have become indispensable analytical platforms for gas detection. However, conventional e-nose systems face significant limitations in portable and wearable implementations due to their bulk and high-power consumption. Herein, a single-sensor-based multifunctional e-nose system is reported by integrating a micro-electromechanical system (MEMS) gas sensor with a flexible printed circuit board (FPCB). Specifically, the ZnO-ZnSnO 3 raspberry-like microspheres (ZZSRM) are utilized as the gas-sensitive materials, and the gas selectivity of the sensor is enhanced through a dual-temperature modulation strategy. Additionally, a gas classification model based on the MiniRocket algorithm has been developed, enabling efficient feature extraction and low-complexity classification of response signals, thereby satisfying the real-time processing demands of embedded devices. Moreover, a silent communication method is proposed, which maps breathing frequency to Morse code for information transmission in specific scenarios. Experimental results demonstrate that the wearable system achieves high-precision classification and concentration prediction for eight volatile organic compounds (VOCs), while simultaneously enabling robust recognition of exhaled signals and instantaneous conversion of Morse code into legible alphabetic characters. By combining the gas sensor with artificial intelligence (AI) technology, this work establishes a multifunctional flexible e-nose that merges portable gas detection and silent communication, offering a novel technological framework for environmental monitoring and human-machine interaction.
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JOURNAL OF BIOTECHNOLOGY
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2016
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IF 2.667
Ping Wei,Jia-Xin Gao,Gao-Wei Zheng,Hong Wu,Min-Hua Zong,Wen-Yong Lou
The novel anti-Prelog stereospecific carbonyl reductase from Acetobacter sp. CCTCC M209061 was successfully expressed in E. coli combined with glucose dehydrogenase (GDH) to construct an efficient whole-cell biocatalyst with coenzyme NADH regeneration. The enzymatic activity of GAcCR (AcCR with a GST tag) reached 304.9 U/g-dcw, even 9 folds higher than that of wild strain, and the activity of GDH for NADH regeneration recorded 46.0 U/mg-protein in the recombinant E. coli . As a whole-cell biocatalyst, the recombinant E. coli BL21(DE3)pLysS (pETDuet- gaccr - gdh ) possessed a broad substrate spectrum for kinds of carbonyl compounds with encouraging yield and stereoselectivity. Besides, the asymmetric reduction of ethyl 4-chloroacetoacetate (COBE) to optically pure ethyl 4-chloro-3-hydroxybutyrate (CHBE) catalyzed by the whole-cell biocatalyst was systematically investigated. Under the optimal reaction conditions, the optical purity of CHBE was over 99% e.e. for ( S )-enantiomer, and the initial rate and product yield reached 8.04 μmol/min and 99.4%, respectively. Moreover, the space-time yield was almost 20 folds higher than that catalyzed by the wild strain. Therefore, a new, high efficiency biocatalyst for asymmetric reductions was constructed successfully, and the enantioselective reduction of prochiral compounds using the biocatalyst was a promising approach for obtaining enantiopure chiral alcohols.
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ACS Applied Materials & Interfaces
·
2017
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IF 7.504
Ruslan G. Tuguntaev,Shizhu Chen,Ahmed Shaker Eltahan,Anbu Mozhi,Shubin Jin,Jinchao Zhang,Chan Li,Paul C. Wang,Xing-Jie Liang
Vitamin E derivatives possess many essential features for drug-delivery applications, such as biocompatibility, stability, improvement of water solubility of hydrophobic compounds, anticancer activity, and the ability to overcome multidrug resistance (MDR). Herein, vitamin E derivatives are used to overcome MDR through a combined P-glycoprotein (P-gp) inhibition and mitochondrial impairment strategy. A novel nanomicellar drug-delivery system as a carrier for doxorubicin (DOX) was developed, in which d-α-tocopheryl polyethylene glycol 1000 succinate was used as a P-gp inhibitor, α-tocopheryl succinate was introduced as a mitochondrial disrupting agent, and d-α-tocopheryl polyethylene glycol 2000 succinate was used as the main building block of micelles. The optimal ratio between the components of the nanocarrier was determined. The resultant DOX-loaded mixed micelles exhibited a suitable size of 52.08 nm, high drug-loading encapsulation efficiency (>98%), high stability, and pH-dependent drug release. In vitro experiments demonstrated a significantly increased cytotoxic activity of DOX-loaded mixed micelles against resistant MCF-7/Adr cells (45-fold higher than DOX after 48 h of treatment). In vivo studies revealed superior antitumor efficiency with less cardio- and hepatotoxicities of DOX-loaded micelles compared with that of free DOX. These results highlight that the developed DOX-loaded mixed micelles have a promising potential to overcome MDR in chemotherapy for clinical usage.
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DRUG DELIVERY
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2020
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IF 4.902
Pengcheng Zhang,He Tao,Liyang Yu,Lixiao Zhou,Chenggang Zhu
Injectable implants with the ability to form in situ are one of the most promising carriers for the delivery of chemotherapeutic drugs to tumor sites. We have reported a novel injectable in situ-forming implant system composed of n-butyl-2-cyanoacrylate (NBCA), ethyl oleate, along with the sol-gel phase transition. The chemotherapeutic drug-loaded injectable NBCA ethyl oleate implant (INEI) exhibited excellent therapeutic efficacy for local chemotherapy. Herein, we utilize this INEI to carry N, N′-(Sulfonyldi-4,1-phenylene)bis(2-chloroacetamide) (TE-C-5003), which is a selective protein arginine methyltransferase 1 (PRMT1) inhibitor, to treat the lung cancer mice model. The in vitro experiment shows that TE-C-5003 has a good anti-tumor effect on lung cancer (IC50 = 0.7022 µM for A549; IC50 = 0.6844 µM for NCL-H1299) and breast cancer (IC50 = 0.4128 µM for MCF-7; IC50 = 0.5965 µM for MDA-MB-231). Anti-tumor experiments in animal models showed that the average growth inhibition rate of xenografted human lung cancer cells by the TE-C-5003-loaded INEI (40% NBCA) was 68.23%, which is far more than TC-E-5003 alone (31.76%). Our study further confirms that INEI is an effective technique to improve the anti-tumor effect. The druggability of small molecule compounds can be improved with the help of the mentioned technology. Also, TC-E-5003 may be developed as a broad spectrum anti-tumor drug.
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Analytical Methods
·
2018
·Vol. 10
·期 22
·2604-2613
·
IF 2.073
Xianxin Qiu,Longjie Zhong,Ying Gan,Kaiqi Su,Shihui Hu,Ping Wang
Okadaic acid is a kind of fat-soluble diarrhetic shellfish poison existing extensively in shellfish. The rapid on site detection of shellfish contaminated by the toxin is a serious problem. Herein, a new method combining a kit with the Bionic e-Eye was developed for the rapid detection of okadaic acid on site. After optimization the kit reached a very low limit of detection, 0.19 μg L−1, and the linear detection range was 0.2–10 μg L−1. The cross reaction rate with dinophysistoxins 1 was 23.6%. The kit had a small cross reaction with other kinds of toxin like saxitoxin, gynotoxin 2 & 3 and pectenotoxins 2-b. The recovery range was about 75.78–102.56%, and the CVs were lower than 20%. A correlation analysis was conducted between the Bionic e-Eye and a microplate reader in PBS and shellfish backgrounds. The detection results of the real shellfish samples by the Bionic e-Eye exhibited great consistency with high performance liquid chromatography. The total time taken to perform the detection was less than 90 minutes. Above all, the method integrated kit with the Bionic e-Eye was very suitable for the rapid detection of okadaic acid on site.
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CHEMICAL ENGINEERING JOURNAL
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2021
·
IF 13.273
Wei Ma,Binghua Yao,Wen Zhang,Yangqing He,Yan Yu,Jinfen Niu
A novel piezocatalytic active membrane based on mixed matrix of exfoliated multi-flaw MoS 2 (E-MoS 2 ) nanosheets and polyvinylidene fluoride (PVDF) was synthesized by electrospinning technique. The composition and structure of the obtained E-MoS 2 /PVDF electrospun fiber membranes (EFMs) were characterized via FESEM, HR-TEM, XRD, FTIR and XPS techniques. The piezocatalytic activity of the EFMs was evaluated by the degradation of antibiotic oxytetracycline (OTC) under ultrasonic irradiation in the dark. The 10.0 wt% E-MoS 2 /PVDF EFMs exhibited superior OTC degradation efficiency of 93.08% within 24 min with pseudo-first-order kinetic constant ( k ) of 0.09124 min −1 , which was 21.1 times higher than that of the pure PVDF EFMs. The superior performance was attributed to the synergetic effect of the embedded E-MoS 2 nanosheets and a high proportion of PVDF β -phase (94.1%) to effectively improve the piezoelectric property of composite. In addition, the ultrathin multi-flaw structure of E-MoS 2 provided abundant catalytic active edge sites and increased the surface charge density, promoting the generation of more ∙OH and ∙O 2 – radicals to degrade OTC pollutants. The degradation intermediates of OTC over 10 wt% E-MoS 2 /PVDF EFMs were detected by HPLC–MS and two main degradation pathways were reasonably proposed. The piezocatalytic activity of 10 wt% E-MoS 2 /PVDF EFMs remained stable after five consecutive cycles. This study is meaningful for exploring the composite membrane piezocatalysts for largescale applications in wastewater treatment.
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Current Drug Delivery
·
2022
·Vol. 19
·期 1
·49-63
·
IF 3.758
Haijun Shen,Qianqian Gao,Tingting Liu,Haoran Wang,Ran Zhang,Jie Zhou,Sihui Ding,Yang Ye,Zhenhua Sun
Background: The combination of photothermal therapy (PTT) and chemotherapy has proven to be a promising strategy for cancer treatment. Various nanomaterials have shown great potential in combination therapy, including gold, graphene oxide, iron oxide, and other nanoparticles. However, their undefinable toxicity in vivo greatly slowed down their development for clinical applications. Objective: The present work aimed to develop a multifunctional nanoparticle for chemo-photothermal therapy composed of acknowledged biocompatible materials. Methods: A novel biocompatible nanoparticle (HIT-NPs) was self-assembled through the intrinsic interaction between D-α-tocopherol Succinate (TOS), human serum albumin (HSA) and indocyanine green (ICG). Doxorubicin (DOX) was then loaded due to the ion pairing between DOX and TOS. The feasibility of combined chemo-photothermal therapy induced by DOX-loaded HIT-NPs was carefully evaluated. Results: In vitro, HIT-NPs showed no cytotoxicity on human normal liver cells (HL-7702 cells) but obvious killing effects on murine breast cancer cells (4T1 cells). The combined chemo-photothermal therapeutic effect on 4T1 cells was successfully obtained. DOX-loaded HIT-NPs could effectively accumulate in 4T1 subcutaneous tumors after intravenous injection, and the tumor temperature rapidly increased under laser exposure, indicating the feasibility of PTT in vivo. Conclusion: The self-assembled HIT-NPs could provide a promising platform for combined chemo- photothermal cancer therapy with full biocompatibility.
研究领域:
肿瘤学
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纳米医学
·
生物材料
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JACS Au
·
2026
·
IF 8.5
Yu Qin,Huiling Liu,Yan Zhang,Fei Li,Zhiwei Deng,Zhengshan Luo,Yuhao Zhuang,Kai Fu,Zhenbo Yuan,Yijian Rao
Sustainable and scalable production of plant natural products remains a major challenge at the interface of synthetic biology, metabolic engineering, and drug discovery, often constrained by plant-membrane-bound cytochrome P450s. To overcome the bottleneck in P450-catalyzed methylenedioxy-bridge (MDB) formation, we designed a concise, modular Escherichia coli pathway to the alkaloid berberine that bypasses multiple native steps and enables high-level accumulation of the P450-dependent intermediate. We then implemented integrated engineering of CjCYP719A1, including expression optimization, structure-guided design, consensus mutagenesis, and machine learning, to obtain a variant with 8.9-fold higher activity and improved thermostability. This enabled a berberine titer of 353 mg/L in scaled-up preparations, the highest reported to date, demonstrating industrial potential. Furthermore, mechanistic studies reveal their improved catalytic activity and thermostability. Together, this work provides a practical framework for functional expression and systematic optimization of plant-derived P450s to enable efficient production of plant natural products in E. coli.
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Foods
·
2026
·Vol. 15
·期 9
·1475
·
IF 6
Ying Liang,Yaqian Yuan,Jia Wang,Wenxue Chen,Weijun Chen,Qiuping Zhong,Jianfei Pei,Chun Chen,Xiong Fu,Rongrong He,Haiming Chen
The primary processing shapes the taste characteristics of coffee beans, while the regulation pathways remain unclear. Coffee beans processed by five methods—dry processing (DP), wet processing (WP), red honey (RH), black honey (BH) and anaerobic fermentation (AF)—were evaluated using electronic tongue analysis, sensory evaluation, and untargeted metabolomics. Sensory evaluation scores for mouthfeel, balance, and overall were higher in BH and AF. Conversely, the WP and DP exhibited heightened bitterness and astringency responses on the electronic tongue sensors, particularly for the former. The multigroup metabolomic comparison identified 808 DMs, and WGCNA revealed eight sensory-related modules containing 467 hub metabolites, mainly amino acids and derivatives, organic acids, alkaloids, and phenolic acids. KEGG analysis demonstrated that pathways such as caffeine metabolism and glycerophospholipid metabolism were the main pathways responsible for the metabolic differences. Further correlation analysis revealed potential flavor components closely associated with key taste characteristics. 1,3,4,5-tetrahydroxycyclohexanecarboxylic acid and Tyr demonstrated positive associations with bitterness, while TPC, TFC, Gly, and Met exhibited negative correlations with bitterness and astringency. Glu demonstrated a positive correlation with umami. These findings elucidate the material basis by which the primary processing modulates non-volatile compounds and taste perception, offering new insights into enhancing coffee quality.
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