BIOMEDICINE & PHARMACOTHERAPY
·
2019
·
IF 3.743
Zhongchao Wang,Ying Yan,Yini Wang,Fei Tong
Introduction The purpose of the experiment was to survey the therapeutic function of resveratrol (RES)-loaded poly(ethylene glycol)-poly(phenylalanine) (PEG-PPhe) on intestinal ischemia/reperfusion injury (II/RI) via the interaction between CSE/H 2 S and iNOS/NO compared to free RES in diabetic rats. Methods Diabetic rats were pretreated with 20 mg/kg of RES or the RES/PEG-PPhe complex and then subjected to 1 h of ischemia and 3 h of reperfusion. Blood and intestines were collected, intestinal pathological injury was estimated, and the contents of body weight, weights of different tissues, blood glucose, serum insulin, HOMA index, serum nitric oxide (NO) and serum sulfureted hydrogen (H 2 S) were observed. The dry/wet intestine ratios, the activity of superoxide dismutase (SOD); the contents of methane dicarboxylic aldehyde (MDA), glutathione (GSH), H 2 S, and NO; and the concentrations of inducible nitric oxide synthase (iNOS) and cystathionine-γ-lyase (CSE) were observed in the intestinal tissues. Results A significant reduction of weights of different tissues, blood glucose, pathological damage, dry/wet ratios, MDA, NO, iNOS expression and a significant increasement of body weight, serum insulin, HOMA index, SOD, GSH, H 2 S, CSE expression were observed in both treatment groups. However, a greater reduction of weights of different tissues, blood glucose (7.49–13.49 mmol/L for 72 h vs. the control) and pathological damage, iNOS expression, dry/wet ratios (6.14 ± 0.29 vs. 8.51 ± 0.42), MDA (5.01 ± 0.71 nmol vs. 9.98 ± 0.67 nmol), NO (0.52 ± 0.09 μmol vs. 0.99 ± 0.08 μmol in intestinal tissue; 19.29 ± 0.89 μmol vs. 45.23 ± 1.17 μmol in serum) was observed in the RES/PEG-PPhe group relative to the I/R ( P < 0.01 for all); a greater increasement of body weight, serum insulin, HOMA index, SOD (39.79±1.78 U vs. 11.84 ± 1.02 U), GSH (31.25 ± 1.19 mg vs. 10.13 ± 0.64 mg), H 2 S (39.52 ± 1.32 nmol vs. 13.02 ± 1.03 nmol in intestinal tissue; 9.78 ± 0.79 μmol vs. 3.11 ± 0.85 μmol in serum), CSE expression was observed in the RES/PEG-PPhe group relative to the I/R ( P < 0.01 for all). In addition, aminoguanidine (AMI, iNOS inhibitor) reduced I/R injury, and dl -propargylglycine (PAG, CSE inhibitor) increased I/R injury. Conclusions The interaction between CSE/H2S and the iNOS/NO-mediated resveratrol/poly(ethylene glycol)-poly(phenylalanine) complex alleviates intestinal ischemia/reperfusion injuries in diabetic rats.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Run-Ze Cao,Zi-Yi Zhang,Jiao Jiao,Qing-Yan Gai,Ying Liu,Yuan Wang,Dan Qu,Yu-Jie Fu
Sporopollenin is a natural biological macromolecule consisting of highly cross-linked carbon, hydrogen, and oxygen atoms, with a highly porous structure and multifunctional groups. In this work, a novel surface molecularly imprinted polymer based on magnetically aminated cattail sporopollenin (MACSp-SMIP) was prepared for the specific and efficient adsorption of resveratrol, with the aim of purifying resveratrol from Polygonum cuspidatum extracts. MACSp-SMIP was found to have a porous structure covered with the multi-layered sponge-like imprinted polymers. MACSp-SMIP had a high adsorption capacity for resveratrol (65.77 mg·g −1 ) and excellent selectivity (imprinting factor 5.64). The adsorption of resveratrol by MACSp-SMIP was a homogeneous diffusion dominated by chemical adsorption with three stages of external diffusion, internal diffusion, and micropore diffusion. MACSp-SMIP was used as an adsorbent in molecularly imprinted solid-phase extraction for the purification of resveratrol from P. cuspidatum extracts, achieving a resveratrol recovery of 94.33 % and a purity of 76.67 % in the final products. MACSp-SMIP maintained a satisfactory recovery of resveratrol (88.18 %) after six cycles. Overall, this work developed a promising biological macromolecule-based adsorbent MACSp-SMIP for the specific and efficient adsorption of resveratrol, and also provided an efficient and simple approach for the selective purification of resveratrol from P. cuspidatum extracts for food/nutraceutical applications.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2024
·
IF 3.1
Jin Shuang,Ren Yubin,Peng Cailiang,Cheng Yupeng,Liu Weili,Fu Yujie,Lv Chen,Cai Hongyao
An efficient and green method was developed using deep eutectic solvent assistance to enhance the biotransformation method of producing resveratrol from Polygonum cuspidatum Siebold & Zucc, using cellulose-based immobilised Aspergillus niger in the process. Various deep eutectic solvents (DES) were screened to obtain a superior biocatalytic effect. The increase in DES concentration aggravated the degree of cell membrane damage. Natural deep eutectic solvents (NADES) exhibited a more favourable catalytic effect than DES due to their excellent biocompatibility. This enhancement is associated with the hydrogen bonding donor components present in NADES, with catalytic ability ranking as alcohol-based > sugar-based > organic acid. CHCL/EG exhibited the maximum catalytic effect at 1.0 wt%. Under optimal conditions (pH 6.5; temperature, 29.5 °C; ratio of liquid to solid 20:1 (mL/g), and time 47 h), the resveratrol yield reached 32.79 mg/g, which was 13.06-fold to that of the untreated sample (2.51 mg/g). The residual activity of the cellulose-based microreactor was 81.46% after ten trials. The proposed method was successfully employed, demonstrating higher biocatalysis efficiencies and superior environmental protection compared to conventional solvents for resveratrol biocatalysis.
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TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
·
2022
·Vol. 21
·期 10
·2139-2146
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IF 0.523
Limei Du,Xinglan Huo,Ying Liu
Purpose: To study the influence of resveratrol on chronic obstruction in Aspergillus fumigatus infection-induced chronic lung disease (COPD) in rats, and the process involved.
Methods: Seventy-five Sprague Dawley SD) rats were assigned to blank control, COPD model, Aspergillus fumigatus-infected COPD model, low (100 mg/kg) and high-dose (200 mg/kg) resveratrol groups, with 15 rats in each group. Enzyme-linked immunosorbent assay (ELISA) was used to assay IL-6, IL-8 and TNF-α in bronchoalveolar lavage fluid (BALF), while lung protein concentrations of AMPK and PGC-1α were assayed by immunoblotting.
Results: In low- and high-dose resveratrol groups, inflammatory factor levels were significantly lower than the corresponding levels in COPD model and Aspergillus fumigatus-infected COPD model (p < 0.05), while lung tissue proteins of AMPK, PGC-1α, and AQP5 of the rats given high-dose resveratrol were significantly raised, relative to corresponding levels in rats given low-dose resveratrol (p < 0.05).
Conclusion: Resveratrol modulates expression levels of secretory factors and surface markers in alveolar macrophages in COPD model rats infected with Aspergillus fumigatus. Resveratrol exerts this effect through the regulation of AMPK/PGC-1α signaling pathway.
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POLYMER DEGRADATION AND STABILITY
·
2021
·
IF 5.03
Zilong Zhang,Xiaohan Li,Ying Bao,Wei Wei,Xiaojie Li,Xiaoya Liu
Development of new allyl compounds to modify bismaleimide (BMI) resins for improving the toughness while maintaining a high thermal performance and flame retardancy is still urgent. In this study, we synthesized the allyl ether of resveratrol (AER), a bio-based allyl compound, to modify BMI resin. A series of BMI/AER resins (BA resins) were prepared by changing the molar ratio of BMI to AER. Compared with 2,2′-diallyl bisphenol A (DBA) modified BMI resins (BD resins), which has been widely used in industry, BA resins can be cured at a lower temperature. Although the toughness of cured BA resins was slightly inferior than that of cured BD resins, the cured BA resins exhibited much superior thermal performance and flame retardancy to the cured BD resins. For the BA-0.8 resin with a molar ratio of imide to allyl groups of 1:0.8, the glass transition temperature, thermal decomposition temperature of 5% weight loss, and char yield of the cured product were as high as 388 °C, 415 °C, and 48.6%, respectively. Meanwhile, the UL-94 and limit oxygen index (LOI) tests revealed an excellent flame retardancy of the cured BA-0.8 resin with V-0 level and LOI of 37.7%. The remarkable properties were attributed to AER with the tri-functionality and stiffness conjugated stilbene backbone giving the cured resins higher crosslinking density, chain rigidity and thermostability compared with DBA. This work provides a novel insight into the development of high-performance bismaleimide-based thermosetting resins with excellent halogen-free and phosphorus-free intrinsic flame retardancy.
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Animal Nutrition
·
2024
·
IF 6.1
Changming Hong,Yujian Huang,Guan Yang,Xiaolu Wen,Li Wang,Xuefen Yang,Kaiguo Gao,Zongyong Jiang,Hao Xiao
Previous studies have shown that maternal resveratrol improved growth performance and altered the microbial composition of suckling piglets under hot summer conditions. However, it remains unclear how maternal resveratrol improves growth performance of suckling piglets under high summer temperatures. A total of 20 sows (landrace × large white; three parity) were randomly assigned to 2 groups (with or without 300 mg/kg resveratrol) from d 75 of gestation to d 21 of lactation in a high temperature environment (from 27 to 30 °C). The results showed that maternal resveratrol supplementation increased total daily weight gain of piglets under hot summer conditions, which is consistent with previous studies. Furthermore, we found that maternal resveratrol improved the intestinal morphology and intestinal epithelial proliferation in suckling piglets. Dietary resveratrol supplementation affected the characteristics of exosome-derived microRNAs (miRNAs) in sow colostrum, as well as the genes targeted by differentially produced miRNAs. MiRNAs are concentrated in the tight junction pathway. As a result, the expression of intestinal tight junction proteins was increased in suckling piglets ( P < 0.05). Notably, maternal resveratrol increased the intestinal secretory immunoglobulin A (sIgA) levels of suckling piglets via colostrum immunoglobins ( P < 0.05), which could increase the abundance of beneficial microbiota to further increase the concentration of short chain fatty acids (SCFA) in suckling piglets' intestine ( P < 0.05). Finally, our correlation analysis further demonstrated the positive associations between significantly differential intestinal microbiota, intestinal sIgA production and SCFA concentrations, as well as the positive relation between total daily weight gain and intestinal health of suckling piglets. Taken together, our findings suggested that maternal resveratrol could promote intestinal health to improve piglet growth under high summer temperatures, which might be associated with the immunoglobin and exosome-derived miRNAs in sows’ colostrum.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2025
·Vol. 2025
·期 1
·9934626
·
IF 4.2
Yingna Zhai,Chunnan Hu,Huitao Li,Yiyan Wang,Shaowei Wang,Xiaoheng Li,Ren-shan Ge,Qiqi Zhu
Introduction: Resveratrol and its analogs have potential therapeutic usage. Resveratrol is metabolized to various metabolites by gut microbiota, including dihydroresveratrol, lunularin, pinostilbene, and oxyresveratrol. However, they might have side effects by inhibiting human gonadal 3β-hydroxysteroid dehydrogenase 2 (h3β-HSD2) and rat homolog r3β-HSD1, thereby interfering with steroid biosynthesis. Methods: Herein, we analyzed the inhibitory strength via in vitro assay, mode of action, structure–activity relationship, and docking simulation of resveratrol analogs on 3β-HSDs. Human KGN cell microsome was used as h3β-HSD2 source, and 90-day-old male Sprague–Dawley rat testicular microsome was used as r3β-HSD1 source. The conversion of pregnenolone to progesterone by 3β-HSDs was analyzed. Results: IC 50 values for h3β-HSD2 were 4,4′-dihydroxystilbene (8.87 μM) > pinostilbene (10.51 μM) > resveratrol (50.04 μM) > lunularin (96.10 μM), while those for r3β-HSD1 were pinostilbene (5.11 μM) > 4,4′-dihydroxystilbene (15.16 μM) > resveratrol (26.58 μM) > lunularin (34.32 μM). Most resveratrol analogs were mixed/competitive inhibitors of both 3β-HSDs. Lipophilicity (LogP) and lowest binding energy determined the inhibitory strength. Docking analysis showed that resveratrol and its analogs bind to the NAD + -/steroid-binding sites of 3β-HSDs. Conclusion: Resveratrol can inhibit both human and rat gonadal 3β-HSDs, thereby interfering with the metabolism and concentrations of steroid hormones such as progesterone, testosterone, and estradiol. Practical Application: Consequently, this interference could hold significance for conditions related to hormone imbalances, such as polycystic ovary syndrome and certain cancers. Disorders marked by elevated levels of androgens, like hyperandrogenism, might find therapeutic benefit from interventions aimed at modulating 3β-HSD activity. Hence, resveratrol and its metabolites could present themselves as natural or supplementary treatment options for managing such conditions.
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JOURNAL OF NUTRITIONAL BIOCHEMISTRY
·
2025
·
IF 4.9
Heng Zhou,Yueming Long,Fangmei Yu,Chenhui Ji,Li Gui,Yunxia Lu
Resveratrol (RSV) ameliorates endothelial dysfunction (ED) primarily through sirtuin 1 (SIRT1). Increasing evidence shows pyroptosis as a novel mechanism in palmitic acid (PA)-induced ED. p66Shc is an adaptor protein involved in oxidative stress. However, whether RSV attenuates the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome via p66Shc remains elusive. This study aims to evaluate whether the anti-pyroptotic effect of RSV and the SIRT1 inhibitor EX527 are related to p66Shc. High-fat diet (HFD) induced obesity in mice, and RSV was administered intragastrically with 400mg/kg/d for 22 successive weeks. The serum levels of interleukin-1β (IL-1β) and IL-18 were analyzed, and the expression of related proteins were assayed with immunohistochemistry in the thoracic aorta. human umbilical vein endothelial cells (HUVECs) were induced by PA, then treated with RSV and EX527 respectively, lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP) and expression of p66Shc, NLRP3, GSDMD and pyroptosis-related genes were assayed. RSV administration ameliorated endothelial cell pyroptosis by decreasing serum IL-1β and IL-18, the expression of NLRP3, p66Shc, and gasdermin D (GSDMD), and increasing the expression of SIRT1 in the HFD-treated thoracic aorta. PA promoted GSDMD-mediated endothelial cell pyroptosis by ROS production, LDH release, decreased MMP and SIRT1 expression, increased expression of p66Shc and activation of the NLRP3 inflammasome in a dose-dependent manner. RSV attenuated PA-induced pyroptosis, whereas EX527 reversed the antipyroptotic effect of RSV in PA-treated HUVECs. Our results suggested a new mechanism that RSV improves PA-induced pyroptosis in endothelial cells via the SIRT1-p66Shc-NLRP3 pathway.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Jing Feng,Zifei Wang,Xiyu Li,Chongyun Bao,Yu Xiao
The management of chronic infected wounds remains a significant clinical challenge, largely due to the deficiency of optimal wound dressings with adequate mechanical strength, appropriate adhesiveness, and efficient sustainable antibacterial, reactive oxygen species (ROS) scavenging, pro-angiogenesis, and immunomodulation properties. To address such a dilemma, we employed a simple and facile strategy to utilize resveratrol (RSV) as a functional component to mediate hydrogel gelation in this study. The structure of this obtained hydrogel was supported by a multibond network, which not only endowed the resultant product with superior mechanical strength and moderate adhesiveness but also effectively prolonged the bioavailability of RSV. This strategy successfully integrated the entire system with sustainable antibacterial, ROS scavenging, pro-angiogenesis, and immunomodulation properties. Subsequent in vivo evidence has verified that this material was capable to accelerate the healing of chronic infected wounds. The underlying mechanism can be explained that this hydrogel is capable of propelling macrophage polarization from the M1 to M2 phenotype through modulating the PI3K/AKT signaling pathway to activate the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling as well as maintaining the mitochondrial membrane potential level in the normal state under excessive inflammatory and oxidative stimulus. In summary, this multifunctional hydrogel wound dressing provides a feasible way to promote the bioavailability of RSV, which is conducive for preparing a promising candidate for chronic infected wound healing. What is more important, it is also beneficial to reveal the correlative mechanisms to establish advanced therapeutic platform for targeting other complex infection microenvironment.
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Food Chemistry-X
·
2026
·
IF 9.2
Yuexin An,Ying Zhu,Xiaoyu Xia,Yuyang Huang,Mingshou Lv,Xiuqing Zhu
Plant protein emulsion gels often show suboptimal mechanical properties. This study aimed to improve the protein secondary structure, rheological behavior, and resveratrol (RES) delivery performance of soybean protein isolate (SPI)–hemp protein isolate (HPI) emulsion gels via carboxymethyl cellulose (CMC) incorporation combined with ultrasound treatment. Initially, the degree of protein–polysaccharide grafting increased with the rise in CMC concentrations before decreasing thereafter. The partial conversion of α-helices to β-sheets exposed hydrophobic groups, facilitating the formation of an interpenetrating protein–polysaccharide network. Rheological analysis revealed that CMC decreased the ellipticity of elastic Lissajous curves and inhibited the linearization of viscous Lissajous curves, thereby improving resistance to the external environment. Moderate ultrasound treatment (400 W) further enhanced the encapsulation efficiency (71.84%), loading capacity (23.46%), and bioaccessibility (40.31%) of RES within the gels. In contrast, excessive ultrasound treatment (800 W) weakened protein–CMC interactions. Overall, combined CMC–ultrasound treatment effectively improved the mechano-structural properties and bioactive compound delivery performance of plant protein emulsion gels.
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JOURNAL OF SEPARATION SCIENCE
·
2024
·Vol. 47
·期 4
·2300811
·
IF 2.8
Run-Ze Cao,Qing-Yan Gai,Jiao Jiao,Yuan Wang,Yu-Jie Fu,Si-Ming Nie,Yao Lu
In this work, surface molecularly imprinted polymers based on magnetic multi-walled carbon nanotubes were prepared for the specific recognition and adsorption of resveratrol. The functionalization of magnetic multi-walled carbon nanotubes and the synthesis process of surface molecularly imprinted polymers were optimized. Characterizations were performed to demonstrate the successful synthesis of the imprinted materials. The imprinted materials showed satisfactory adsorption capacity of resveratrol (45.73 ± 1.72 mg/g) and excellent selectivity (imprinting factor 2.89 ± 0.15). In addition, the imprinted materials were used as adsorbents in molecularly imprinted solid-phase extraction for the purification of resveratrol from crude extracts of some food and medicinal resources, achieving recoveries of 93.69%−95.53% with high purities of 88.37%−92.33%. Moreover, the purified products exhibited extremely strong free radical scavenging activity compared with crude extracts. Overall, this work provided a promising approach for the highly selective purification of resveratrol from natural resources, which would contribute to the application of this valuable compound in the food/nutraceutical fields.
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DRUG METABOLISM AND DISPOSITION
·
2016
·Vol. 44
·期 4
·485-488
·
IF 3.21
Shuai Wang,Feng Li,Enxi Quan,Dong Dong,Baojian Wu
Resveratrol undergoes extensive metabolism to form biologically active glucuronides in humans. However, the transport mechanisms for resveratrol glucuronides are not fully established. Here, we aimed to characterize the efflux transport of resveratrol glucuronides using UGT1A1-overexpressing HeLa cells (HeLa1A1 cells), and to determine the contribution of multidrug resistance–associated protein (MRP) 4 to cellular excretion of the glucuronides. Two glucuronide isomers [i.e., resveratrol 3-O-glucuronide (R3G) and resveratrol 4′-O-glucuronide (R4′G)] were excreted into the extracellular compartment after incubation of resveratrol (1–100 μM) with HeLa1A1 cells. The excretion rate was linearly related to the level of intracellular glucuronide, indicating that glucuronide efflux was a nonsaturable process. MK-571 (a dual inhibitor of UGT1A1 and MRPs) significantly decreased the excretion rates of R3G and R4′G while increasing their intracellular levels. Likewise, short-hairpin RNA (shRNA)–mediated silencing of MRP4 caused a significant reduction in glucuronide excretion but an elevation in glucuronide accumulation. Furthermore, β-glucuronidase expressed in the cells catalyzed the hydrolysis of the glucuronides back to the parent compound. A cellular pharmacokinetic model integrating resveratrol transport/metabolism with glucuronide hydrolysis/excretion was well fitted to the experimental data, allowing derivation of the efflux rate constant values in the absence or presence of shRNA targeting MRP4. It was found that a large percentage of glucuronide excretion (43%–46%) was attributed to MRP4. In conclusion, MRP4 participated in cellular excretion of R3G and R4′G. Integration of mechanistic pharmacokinetic modeling with transporter knockdown was a useful method to derive the contribution percentage of an exporter to overall glucuronide excretion.
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