MOLECULAR AND CELLULAR ENDOCRINOLOGY
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2022
·
IF 4.369
Yuanfei Ren,Maowei Yang,Xindong Wang,Buxuan Xu,Zerong Xu,Bo Su
Diabetic osteoporosis (DOP) is a complication of diabetes mellitus (DM) and occurs due to alterations in bone metabolism under hyperglycemic condition. ELAV-like RNA binding protein 1 (ELAVL1) is abnormally up-regulated in diabetes-related diseases. Bioinformatics prediction indicates that divalent metal transporter 1 (DMT1) is a potential target of ELAVL1 . To explore the role of ELAVL1 and the involvement of ELAVL1/DMT1 axis in DOP, we established a mouse model of DM by administration of high-fat diet and intraperitoneal injection with streptozotocin (STZ). The expression of ELAVL1 and DMT1 was increased in the bone tissues of DM mice. Knockdown of ELAVL1 reduced iron level and oxidative stress , promoted osteogensis, and prevented bone mass loss, thus mitigating DOP in DM mice. In vitro , mouse pre-osteoblast MC3T3-E1 cells were treated with high glucose (25 mM) and ferric ammonium citrate (FAC, 200 μM). The inhibitory effects of ELAVL1 knockdown on iron accumulation and oxidative stress were evidenced in MC3T3-E1 cells. Knockdown of ELAVL1 enhanced osteoblast viability, differentiation and mineralization . Notably, the expression of DMT1 was positively correlated with that of ELAVL1 in vivo and in vitro . Overexpression of DMT1 abolished the effect of ELAVL1 knockdown on the behaviors of MC3T3-E1 cells, suggesting that ELAVL1 might function through regulating DMT1. In conclusion, knockdown of ELAVL1 likely alleviated DOP by inhibiting iron overload and oxidative stress and promoting osteogenesis, and DMT1 might be involved in this process. These findings provide insights into the pathogenesis of DOP and suggest a potential therapeutic target for DOP treatment.
研究领域:
内分泌学
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分子生物学
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骨代谢
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糖尿病
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骨质疏松
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Materials Science and Engineering B-Advanced Functional Solid-State Materials
·
2022
·
IF 3.407
Hua Jiao,Xuerui Zhou,Sen Song,Jiechen Jin,Xinyuan Zhang,Yufei Tang,Kang Zhao
In this study, the tetragonal phase barium titanate ( T -BT) with (1 0 1) facet could be prepared by a simple rotary evaporation precursor system at 800 ℃ for 2 h. (1 0 1) T -BT was modified by photodeposition, and then Ag nanoparticles with diameter of 10–20 nm were grown on the surface of (1 0 1) T -BT to obtain T -BT/Ag. The samples were analyzed by XRD, SEM, XPS and TEM. The photocatalytic performance of T -BT and T -BT/Ag was measured by degradation of MO (Methyl Orange) and RhB (Rhodamine B). T -BT could degrade 1.95 % of MO or 1.09 % of RhB under the parameter of 0.01 mol/L-2 min within 120 min. However, under the same conditions, T -BT/Ag composite powder had the best degradation efficiency, which could degrade 86.7 % of MO or 63.8 % of RhB respectively. The photoluminescence spectrum, electrochemical impedance spectrum, ultraviolet visible diffuse reflectance spectroscopy analysis and Density Functional Theory (DFT) calculation results showed that the O on the surface of (1 0 1) crystal facet of T -BT could bond with (1 1 1) Ag and reached the most stable state.++++.
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APPLIED SURFACE SCIENCE
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2022
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IF 7.392
Yingyue Hu,Xinyao Hu,Lu Xue,Baolong Cui,Yi Du
The modulation of crystal surface is one of the important means of photocatalyst modification. BiOBr photocatalyst with exposed (1 1 0) facets was synthesized at room temperature and pressure using CaCO 3 as a directing agent in this experiment. The addition of CaCO 3 not only promoted the exposed facets of BiOBr from (1 0 2) to (1 1 0), but also improved its ability of photocatalytic degradation of pollutants, meanwhile, changed the specific surface area of BiOBr, provided more active sites, and enhanced the adsorption capacity of BiOBr. At the same time, the effects of different alkaline earth metals on the performance of BiOBr photocatalytic materials were compared, it was determined that the degradation rate of RhB of BiOBr photocatalyst reached 97% within 60 min when adding 500 wt% CaCO 3 , and the main active group in the degradation experiment was h + . In conclusion, BiOBr with different main exposed facets was prepared in this paper, and a new idea was provided for alkaline earth metals in the field of photocatalysis.
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ChemCatChem
·
2022
·Vol. 14
·期 7
·e202101728
·
IF 5.497
Shiqing Wang,Xiaocheng Lan,Boyang Liu,Babar Ali,Tiefeng Wang
Graphical Octylamine synthesis : Ni x Fe 1 /Al 2 O 3 was used as catalyst for 1-octanol amination and provided the highest yield among the reported nonprecious metal catalysts. The generation of Ni−FeO x interactions provided sufficient Fe 2+ /Fe 3+ −O 2− sites for the activation of 1-octanol. Meanwhile, the cooperation between Ni site and Lewis acid-base pairs significantly enhanced the amination of 1-octanol to 1-octylamine. The synthesis of 1-octylamine, a primary platform molecule, remained a great challenge in catalysis. In this work, Ni x Fe 1 /Al 2 O 3 was used for direct amination of 1-octanol to 1-octylamine. Catalyst evaluation indicated that the promotion effect of Fe/Ni ratio on the catalytic amination activity followed a volcano curve while the selectivity to 1-octylamine was positively correlated with the Fe loading. Compared with the Ni/Al 2 O 3 catalyst, the amination activity was increased by 2.8 times by optimizing the Fe/Ni ratio to 0.33, and the selectivity to 1-octylamine was increased from 84.0 % to 96.3 % when increasing the Fe/Ni ratio from 0 to 1. Based on catalyst characterizations, the good performance of Ni x Fe 1 /Al 2 O 3 was ascribed to the combined modification effect of the NiFe alloy and Ni−FeO x interface. The FeO x species enriched on the Ni particle surface provided sufficient Fe 2+ /Fe 3+ −O 2− sites for the activation of 1-octanol, and the cooperation between Ni sites and Lewis acid-base pairs enhanced the amination of 1-octanol to 1-octylamine. This work sheds new light on the design of Ni-based catalysts without using a noble metal for primary amine preparation.
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CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 18
·期 5
·e2100128
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IF 2.408
Shu-Hua Zhang,Jiewei Yang,Hao Ma,Yu Yang,Guo-Feng Zhou,Xue Zhao,Rui Xu,Dan Nie,Guo-Gang Zhang,Jun-Jie Shan,Cheng-Bin Cui,Chang-Wei Li
Five new peptaibols, longibramides A–E ( 1 – 5 ) with 11 amino acid residues, were isolated from a fungus Trichoderma longibrachiatum Rifai DMG-3-1-1, which was isolated from a mushroom Clitocybe nebularis (Batsch) P. Kumm collected from coniferous forest in the subboreal area of northeast China. The structures of longibramides A–E were determined by their spectroscopic data (NMR and MS-MS spectra), their absolute configurations were determined by X-ray diffractions and Marfey's analyses. The X-ray diffractions of longibramides A, B, and the similar CD spectra of A–E showed that they all had α -helix conformations. Longibramides B and E showed moderate cytotoxicities against BV2 and MCF-7 cells and also showed some inhibitory effects against methicillin-resistant Staphylococcus aureus MRSA T144. L- trans- Hyp was not commonly found in natural peptaibols, which was the 6 th or 10 th amino acid residue in longibramides C–E. The X-ray diffractions of longibramides A and B afforded the accuracy conformations of their secondary structures, which maybe help to interpret the structure-activity relationships of the family of peptaibols in the future. Graphical
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Oxidative Medicine and Cellular Longevity
·
2021
·
IF 6.543
Zhenling Zhang,Lijing Zhang,Qiuping Zhang,Bojia Liu,Fang Li,Yi Xin,Zhijun Duan
Background. Intestinal barrier injury is an important contributor to many diseases. We previously found that heme oxygenase-1 (HO-1) and carbon monoxide (CO) protect the intestinal barrier. This study is aimed at elucidating the molecular mechanisms of HO-1/CO in barrier loss. Materials and Methods. We induced gut leakiness by injecting carbon tetrachloride (CCl4) to wildtype or intestinal HO-1-deficient mice. In addition, we administrated tumor necrosis factor-α (TNF-α) to cells with gain- or loss-of-HO-1 function. The effects of HO-1/CO maintaining intestinal barrier integrity were investigated in vivo and in vitro. Results. Cobalt protoporphyrin and CO-releasing molecule-2 alleviated colonic mucosal injury and TNF-α levels; upregulated tight junction (TJ) expression; and inhibited epithelial IκB-α degradation and phosphorylation, NF-κB p65 phosphorylation, long MLCK expression, and MLC-2 phosphorylation after administration of CCl4. Zinc protoporphyrin completely reversed these effects. These findings were further confirmed in vitro, using Caco-2 cells with gain- or loss-of-HO-1-function after TNF-α. Pretreated with JSH-23 (NF-κB inhibitor) or ML-7 (long MLCK inhibitor), HO-1 overexpression prevented TNF-α-induced TJ disruption, while HO-1 shRNA promoted TJ damage even in the presence of JSH-23 or ML-7, thus suggesting that HO-1 dependently protected intestinal barrier via the NF-κB p65/MLCK/p-MLC-2 pathway. Intestinal HO-1-deficient mice further demonstrated the effects of HO-1 in maintaining intestinal barrier integrity and its relative mechanisms. Alleviated hepatic fibrogenesis and serum ALT levels finally confirmed the clinical significance of HO-1/CO repairing barrier loss in liver injury. Conclusion. HO-1/CO maintains intestinal barrier integrity through the NF-κB/MLCK pathway. Therefore, the intestinal HO-1/CO-NF-κB/MLCK system is a potential therapeutic target for diseases with a leaky gut.
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Molecular Catalysis
·
2020
·
IF 3.687
Huan Fu,Luna Ruan,Jianhua Liao,An Pei,Jun Liu,Li Zeng,Kai Yang,Lihua Zhu,Bing Hui Chen
In this report, the Ni/C sample was successfully prepared at room temperature (RT) by depositing nickel related species on carbon black by a chemical liquid reduction method with hydrazine hydrate as reducing agent, Pt/C was synthesized via a wetness impregnation method. And Pt was supported on Ni/C by galvanic replacement reaction to form PtNi/C bimetallic nanocatalyst. By comparing the catalytic performance of different catalysts for 1-nitronaphthalene hydrogenation, it could be found that the PtNi/C bimetallic nanocatalyst showed higher catalytic activity than monometallic nanocatalysts (Pt/C and Ni/C), exhibiting 100 % conversion of 1-nitronaphthalene, 100 % selectivity to 1-naphthylamine and turnover frequency of 194.9 h −1 under mild reaction conditions (40 min, 30 °C, 3.0 MPa H 2 ). The as-prepared catalysts were characterized by XRD, XPS, HAADF-STEM, STEM-EDX mapping and line-scanning, TG, HRTEM and H 2 -TPD, which proved that PtNi/C showed most outstanding catalytic behaviors in 1-nitronaphthalene selective hydrogenation due to high dispersion of platinum, the nanostructure of Pt-on-Ni-on-Ni(OH) 2 and thus synergism effect among Pt, Ni and Ni(OH) 2 .
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2018
·Vol. 135
·期 6
·3005-3013
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IF 2.209
Luo Liqiong,Jin Bo,Peng Rufang,Shang Yu,Xiao Lipengcheng,Chu Shijin
A new nitrogen-rich energetic salt of bis-1-methylimidazole 1 H ,1′ H -5,5′-bistetrazole-1,1′-diolate salt, (1- M ) 2 BTO, was synthesized and characterized (FT-IR, 1 H NMR, 13 C NMR, elemental analysis, and X-ray single-crystal diffraction). Results indicated that (1- M ) 2 BTO crystallizes in the triclinic space group P -1. The thermal decomposition behavior of (1- M ) 2 BTO was determined by differential scanning calorimetry (DSC) and thermogravimetric tandem infrared spectroscopy. The decomposition peak temperature of (1- M ) 2 BTO was 530 K, which suggested that the salt is strong heat resistance. The apparent activation energies were 130.56 kJ mol −1 (Kissinger’s method) and 132.50 kJ mol −1 (Ozawa’s method), respectively. The enthalpy of formation for the salt was calculated as 917.3 kJ mol −1 . The detonation velocity and detonation pressure of (1- M ) 2 BTO were 7448 m s −1 and 20.7 GPa, respectively, using the Kamlet-Jacobs equation. Furthermore, the sensitivity test results showed that its impact sensitivity is greater than 50 J and friction sensitivity is 180 N, indicating that it has a lower sensitivity.
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eLife
·
2024
·
IF 6.4
Shu-Ting Zhang,Shi-Kai Deng,Tao Li,Megan E Maloney,De-Feng Li,Jim C Spain,Ning-Yi Zhou
1-Naphthylamine (1NA), which is harmful to human and aquatic animals, has been used widely in the manufacturing of dyes, pesticides, and rubber antioxidants. Nevertheless, little is known about its environmental behavior and no bacteria have been reported to use it as the growth substrate. Herein, we describe a pathway for 1NA degradation in the isolate Pseudomonas sp. strain JS3066, determine the structure and mechanism of the enzyme NpaA1 that catalyzes the initial reaction, and reveal how the pathway evolved. From genetic and enzymatic analysis, a five gene-cluster encoding a dioxygenase system was determined to be responsible for the initial steps in 1NA degradation through glutamylation of 1NA. The γ-glutamylated 1NA was subsequently oxidized to 1,2-dihydroxynaphthalene which was further degraded by the well-established pathway of naphthalene degradation via catechol. A glutamine synthetase-like (GS-like) enzyme (NpaA1) initiates 1NA glutamylation, and this enzyme exhibits a broad substrate selectivity toward a variety of anilines and naphthylamine derivatives. Structural analysis revealed that the aromatic residues in the 1NA entry tunnel and the V201 site in the large substrate-binding pocket significantly influence NpaA1’s substrate preferences. The findings enhance understanding of degrading polycyclic aromatic amines, and will also enable the application of bioremediation at naphthylamine contaminated sites.
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REPRODUCTIVE TOXICOLOGY
·
2025
·
IF 2.8
Huage Liu
Thirdhand smoke (THS), a residual cigarette smoke still remaining in indoor environment long after active smoking has ceased, has aroused more and more people’s attention on health. However, the impact of THS exposure on the male reproductive system, particularly in in-vivo study, remain unclear. Thus, this study aims to investigate the adverse effects of 1-(N-methyl-N-nitrosamino)-1-(3-pyridinyl)-4-butanal (NNA), a special component in THS, on testis. Herein, adult ICR mice were orally administered diluted dimethylsulfoxide (control), 0.075 μg/kg and 0.15 μg/kg NNA for 10 weeks. The findings revealed that NNA exposure, especially in 0.15 μg/kg dosage, significantly reduced the epithelium height and tubular area within the testis. Transcriptomic analysis indicated that NNA exposure interfered with the expression of genes associated with oxygen transport and oxygen binding processes, such as Hbb-bs , the downregulation of which also led to oxidative damage. Additionally, NNA exposure induced oxidative damage in testis by decreased levels of SOD and GSH enzymes, along with an increase in MDA level. In summary, our results demonstrate that NNA exposure causes impaired testicular structure and oxidative damage, indicating its testicular toxicity. This finding not only implies the detrimental effect of NNA on male reproduction, but also provides a fundamental basis for further research on the impact of THS exposure on male reproductive health.
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POLYHEDRON
·
2024
·
IF 2.4
An-Na Chen,Yan-Ling Deng,Chun-Yang Pan
Two imidazole-based ligand-nickel borates, [Ni(1-ipIM) 3 ·B 6 O 7 (OH) 6 ]·[H 3 BO 3 ]·2·.5H 2 O ( 1 , 1-ipIM = 1-isopropylimidazole) and [Ni(1-pIM) 6 ]·[B 5 O 6 (OH) 4 ]·2H 2 O ( 2 , 1-pIM = 1-propylimidazole) have been synthesized and structurally characterized by single-crystal X-ray diffraction, PXRD, FTIR. The oxoboron cluster structures of 1 and 2 were modulated through changing the carbon-branched structure on the N -site of the imidazole-based ligand using metallic nickel as the central atom. It is noteworthy that the structure of 1 is a hybridized skeleton formed by N -Ni-O-B bonds. The electrocatalytic oxygen reduction reaction (ORR) activity of these borate-based catalysts was investigated. The half-wave potential ( E 1/2 ) values of 1 -based and 2 -based were about 0.825 and 0.80 V, respectively.
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FOOD AND CHEMICAL TOXICOLOGY
·
2019
·
IF 3.775
Congyong Sun,Wenjing Li,Yingkun Liu,Wenwen Deng,Michael Adu-Frimpong,Huiyun Zhang,Qilong Wang,Jiangnan Yu,Ximing Xu
1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside (MPG) is a phenolic glycoside that exists in Moringa oleifera seeds with various health benefits, whereas its hepatoprotective effect is lacking clarification. Herein, MPG was isolated from Moringa oleifera seeds, and its hepatoprotection against CCl 4 -induced hepatotoxicity in L02 cells and ICR mice was investigated. Toxicity studies showed that MPG did not induce significant changes in organ coefficients and histological analysis, as well as exhibited no cytotoxicity. In vitro studies indicated that MPG substantially increased cell viability and intracellular SOD activities, and significantly inhibited LDH leakage in CCl 4 -treated cells. In vivo studies demonstrated that MPG significantly alleviated CCl 4 -induced hepatotoxicity in mice, as indicated by diagnostic indicators of hepatic injury, as well as the histopathological analysis. Moreover, MPG reduced the lipid peroxidation levels and regulated the inflammatory cytokines. Notably, MPG substantially suppressed the significant elevation of ROS production in hepatocytes of mice intoxicated with CCl 4 . Moreover, TUNEL assay demonstrated that MPG obviously inhibited hepatic apoptosis induced by CCl 4 . Altogether, these results suggested that MPG has excellent liver-protecting effects against hepatocytotoxicity induced by CCl 4 in mice and L02 cells, which can be further developed as a valuable functional food additive or drug for the treatment of hepatic injury.
研究领域:
药理学
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毒理学
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肝脏病学
·
营养保健品
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PubMed
高影响因子
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