ANALYTICA CHIMICA ACTA
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2019
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IF 5.256
Xiuzhen Qiu,Weimao Chen,Yiling Luo,Yulin Wang,Yanlian Wang,Huishi Guo
α-amanitin is the most toxic amanita in mushrooms with lethal dose to humans around 0.1 mg. Kg −1 . Hence, early identification of the poison would improve survival rates and prevent lethal poisoning cases. In this study, molecularly imprinted photonic crystal (MIPC) sensor was prepared by combining molecular imprinting with photonic crystal templates and tested towards the detection of α-amanitin. In this process, synthesized moiety of α–amanitin was utilized as template, dispersed SiO 2 colloidal photonic crystal as carrier, methacrylic acid (MAA) as functional monomer , and ethylene glycol dimethacrylate (EDGMA) as crosslinker. The adsorption behavior of MIPC towards α-amanitin in ethanol solution showed shifts in diffraction peaks of MIPC upon binding with α-amanitin molecules. The reflection peak wavelength varied linearly with α-amanitin concentration according to the correlation formula: λ = 15.417 c +489.17 (R 2 = 0.9985). The recognition process was accompanied by gradual color change in MIPC film. The prepared MIPC sensor possessed wide linear range (10 −9 –10 −3 mg L −1 ), change in visual color, low detection limit (10 −10 mg L −1 ), short response time (2 min), and good reusability. The MIPC film was then tested towards the detection of α-amanitin in real biological samples (mushroom, urine, and serum) and showed reasonable shift in diffraction peaks and color change upon soaking in solutions spiked with α-amanitin at 10 −6 mg L −1 and 10 −3 mg L −1 , suggesting the suitability of the film for the rapid identification of α-amanitin in complex sample matrices. Overall, the proposed sensor looks promising for the rapid identification of α-amanitin in clinical analysis and food poisoning.
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高影响因子
BIOORGANIC & MEDICINAL CHEMISTRY
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2016
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IF 2.923
Mankun Wei,Wei-Ming Chai,Rui Wang,Qin Yang,Zhihong Deng,Yiyuan Peng
In this study, eight quinazolinone derivatives were designed and synthesized. Their inhibitory activities on α-glucosidase were assessed in vitro. Two compounds: 2-(4-chlorophenyl)-quinazolin-4(3 H )-one (CQ) and 2-(4-bromophenyl)-quinazolin-4(3 H )-one (BQ) were found to be potent inhibitors of α-glucosidase with IC 50 values of 12.5 ± 0.1 μM and 15.6 ± 0.2 μM, respectively. Spectroscopy methods were performed to analyze the inhibitory mechanisms of both compounds on α-glucosidase. The results revealed that they reversibly inhibited α-glucosidase in a non-competitive manner. CQ and BQ could statically quench the fluorescence spectra by formation of an inhibitor-α-glucosidase complex. The interaction between CQ and α-glucosidase depended on hydrogen bonds , electrostatic and hydrophobic force, while the driving force of the binding between BQ and the enzyme was hydrophobic. The docking results showed that BQ was less active than CQ against α-glucosidase because of its weaker interaction with the enzyme. In brief, the quinazolinone derivatives identified in this work were potentially promising candidates for developing as novel anti-diabetic agents.
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高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2019
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IF 4.833
Yuxuan Dai,Na Yue,Junni Gong,Chunxia Liu,Qifei Li,Jiaqi Zhou,Wenlong Huang,Hai Qian
Proteolysis-targeting chimera (PROTAC) could selectively degrade target protein and may become a promising strategy for treating estrogen receptor α (ERα) positive breast cancers. Here, we designed penetrated peptide-based PROTACs by constructing an N-terminal lactam cyclic to improve proteolytic stability and cell penetration. We used a lactam cyclic peptide as ERα binding ligand , 6-aminocaproic acid as a linker, and a hydroxylated pentapeptide structure for recruiting E3 ligase to obtain heterobifunctional compounds. The resulting optimized compound I-6 selectively recruited ERα to the E3 ligase complex for promoting the degradation of ERα. Compound I-6 possessed strong effect on MCF-7 cell toxicity (IC 50 ∼9.7 μM) and significantly enhanced activities in inducing ERα degradation. Meanwhile, I-6 performed much stronger potency in inhibition of tumors growth than tamoxifen . This work is a successful template to construct PROTACs based on cell-permeable peptides, which could extend the chemical space of PROTACs.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Yu Zheng,Ruifen Zhang,Fei Huang,Lai-Hoong Cheng,Liangxiong Xu,Xuchao Jia
Various studies have documented the beneficial effects of black soybean extract against diabetes. However, the specific active compounds responsible for these effects remain elusive. Here, we aimed to identify the α-glucosidase inhibitors present in black soybeans using affinity ultrafiltration. Seven isoflavones with α-glucosidase inhibitory activity were identified, among which daidzein and genistein demonstrated remarkably superior inhibitory activity compared to that of acarbose (IC 50 = 632.5 ± 70.0 μmol/L), exhibiting IC 50 values of 15.7 ± 0.3 and 3.2 ± 1.2 μmol/L, respectively. An analysis of the structure-activity relationship revealed a negative correlation between the degree of glycosylation of isoflavone and α-glucosidase inhibitory activity. Enzyme kinetic studies revealed that both daidzein and genistein acted as uncompetitive inhibitors of α-glucosidase. Additionally, these compounds effectively suppressed the intrinsic fluorescence of α-glucosidase through the static quenching mechanism. The interaction between daidzein or genistein and α-glucosidase involves spontaneous formation of a complex, which is driven by hydrophobic interactions and hydrogen bonds. These findings provide valuable insights into the molecular mechanisms underlying the α-glucosidase inhibitory activity of black soybean isoflavones.
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2023
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IF 5
Yang Xiaoling,Zhao Shunan,Deng Yong,Xu Weidong,Wang Zonghan,Wang Wenjun,Lv Ruiling,Liu Donghong
Abstract This study aimed to evaluate the antibacterial activities of α-terpineol against common foodborne pathogenic bacteria by agar well diffusion, broth microdilution, and colony counting assay. Propulsive research was conducted to reveal the antibacterial mechanisms, including morphology, infrared spectroscopy, membrane fluidity, membrane permeability, proton motive force, and oxidative phosphorylation. Results indicated that the antibacterial activity of α-terpineol decreased in the following order: Escherichia coli O157:H7, Salmonella typhimurium , Listeria monocytogenes , and Staphylococcus aureus . With an initial cell count of 8 log CFU/mL, α-terpineol at 0.8% (v/v) reduced E. coli O157:H7 and S. aureus by approximately 5.6 and 3.9 log CFU/mL within 1 h, respectively. Remarkable destruction in cell envelopes and intracellular organizations was observed. The hydroxyl of α-terpineol might form glycosidic bonds with carbohydrates and hydrogen bonds with PO 2 − and COO − via infrared spectroscopy analysis. Generalized polarization of Laurdan revealed that the polar head groups of phospholipids transformed into close packed. The anisotropy variations of trimethyl amino-diphenylhexatriene (TMA-DPH) and DPH suggested membrane fluidity decreased. The N-phenyl-1-naphthylamine intake assay indicated that α-terpineol impaired the cell wall. Propidium iodide staining was indicative of damaged plasma membranes. Electron transport in the cytoplasmic membrane was impaired, inducing reactive oxygen species accumulation. Both membrane electrical potential and membrane pH gradient collapsed. The disruption of proton motive force and the leakage of ATP resulted in a deficit of intracellular ATP. Our research revealed the interaction between the hydroxyl group of α-terpineol and bacteria affects membrane function contributing to the bacteria’s death. Key points • α-Terpineol hydroxy formed glycosidic bonds and hydrogen bonds with bacteria • α-Terpineol increased the membrane gelation and reduced the membrane fluidity • Proton motive force and oxidative phosphorylation were impaired Graphical abstract
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Arabian Journal of Chemistry
·
2023
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IF 6
Haijun Cheng,Min Liang,Suyi Dai,Xianrui Lu,Qilin Huang,Fang Lai,Li Ma,Weiguang Li,Xiongmin Liu
α-Pinene, β-pinene and turpentine are renewable resources that are widely used in fine chemicals, fuels and other applications, and the safety of their production, transport and use should be considered. In this work, the characteristics and hazardous of the oxidation process of α-pinene, β-pinene and turpentine were investigated by using a novel mini closed pressure vessel testing system (MCPVT). The oxidation products were resolved by gas chromatography-mass spectrometry (GC–MS). The results showed that α-pinene, β-pinene and turpentine were stable under nitrogen atmosphere and very active chemically under oxygen atmosphere. The activation energies ( E a ) of α-pinene, β-pinene and turpentine were calculated to be 78.87, 109.69 and 98.82 kJ/mol, respectively, using pressure–time (P-t) curves. So, the reaction activity sequence was α-pinene > turpentine > β-pinene. The peroxide concentrations of α-pinene (363 K), β-pinene (358 K) and turpentine (363 K) could reach 81.94, 84.70 and 75.76 mmol/kg, respectively, at relatively low temperatures. 0.6 g of α-pinene exploded at a system temperature of 369 K and an oxygen pressure of 1.23 MPa. 0.6 g of β-pinene also exploded at a system temperature of 373 K and an oxygen pressure of 1.27 MPa. 0.4 g of turpentine experienced thermal runaway at a system temperature of 384 K and an oxygen pressure of 0.81 MPa. The major oxidation products of α-pinene are α-campholenaldehyde, laevo-pinocarveol, verbenol, verbenone, bicyclo (3.1.1)heptane-2, 3-diol, 2, 6, 6-trimethyl, 2-cyclohexen-1-ol, 2-methyl-5-(1-methylethenyl)-, (1R,5R)-rel-, cyclobutaneacetic acid, 3-acetyl-2, 2-dimethyl, myrtenol and myrtenal. The major thermal runaway products are water, ethanol, acetone, acetic acid, 2-pentanone, propyl acetate, toluene, cymene and methylacetophenone. The major oxidation products of β-pinene are nopinone, laevo-pinocarveol, pinocarvone, myrtenol, myrtenal and perillyl alcohol. The major thermal runaway products are water, ethanol, acetone, n-ethyl propanoate, toluene, butyl acetate, cymene, apocamphor, nopinone, bornyl formate, methylacetophenone and dihydronopol. The oxidation products and thermal runaway products of pinene were significantly different. Possible reaction pathways for α-pinene and β-pinene are proposed.
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高影响因子
ANALYTICAL CHEMISTRY
·
2023
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IF 7.4
Ting Li,Wei Lu,Hui Tian,Yu Cao,Qianqian He,Xia Chen,Hailong Wang
The antitumor effect of Pt-based drugs is determined by their binding activity with deoxyribonucleic acid (DNA), and understanding the reaction process in a systematic manner is crucial. However, existing assays used for DNA–Pt research suffer from several issues, such as complicated sample preparation, preamplification, and expensive instruments, which dramatically limit their practical application. In this study, a novel method was presented to investigate the adducts of DNA and oxaliplatin using an α-hemolysin nanopore sensor. This approach allows for real-time monitoring of the DNA–oxaliplatin condensation process through the detection of nanopore events associated with DNA–oxaliplatin adducts. Specifically, type I and II signals exhibiting specific current characteristics were observed during the process. Typical signals with high frequency were obtained by recording the designed DNA sequence. Furthermore, the production of these signals was confirmed to be independent of homologous adducts. This finding suggests that the DNA–oxaliplatin adduct can serve as a potential sensor for detecting oxaliplatin lesions and multiple types of molecules.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
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2023
·Vol. 58
·期 12
·5319-5333
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IF 4.5
Wang Yun,Tan Hongjie,Sang Lexian,Xie Yunying,Jia Fuchao,Zhou Tong,Yin Guangchao,Sun Fazhe,Wang Xiaomei
An optimized acetone gas sensor based on Ti 3 C 2 / α -Fe 2 O 3 (TF) composite materials was synthesized using microwave and etching methods. The structures, morphology and composition of the TF composites were characterized via XRD, XPS, SEM and TEM. The crystal of α -Fe 2 O 3 porous nanorods is hexagonal, and the α -Fe 2 O 3 nanoclusters formed from α -Fe 2 O 3 nanorods were adhered adequately on the lamellar Ti 3 C 2 MXene in the TF composite. The specific surface area and surface defects of the TF composites increased compared with pure α -Fe 2 O 3 nanocluster material, thus exposing a large number of gas adsorption sites. Furthermore, the excellent electrical conductivity and high free charges transfer ability of Ti 3 C 2 MXene provided more electrons for the gas-sensitive reaction and enhanced the transmission capacity of gas-sensitive reaction electrical signal, respectively. The Schottky barriers were formed at the interface of α -Fe 2 O 3 and Ti 3 C 2 MXene increasing the resistance of TF gas sensor in air, which is beneficial to the response of TF gas sensor. So, the gas-sensitive performance of TF gas sensor to acetone was greatly improved. The response of TF gas sensor to 100 ppm acetone was up to 23.38, which was nearly 2.3 times higher than pure α -Fe 2 O 3 gas sensor. The lower detection limit of TF gas sensor could be as low as 1 ppm. In addition, the TF gas sensor had excellent selectivity, long-term repeatability and stability for acetone.
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ACS Applied Nano Materials
·
2023
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IF 5.9
Qunling Fang,Jing Wang,Qingshan Xiong,Yunqi Xu,Guangjin Yu,Ailing Hui,Ken Cham-Fai Leung,Shouhu Xuan
Multifunctional photothermal nanomaterials offer notable opportunities for antimicrobial therapy due to their excellent antibacterial activities without leading to drug resistance. Herein, we report a facile one-step strategy for fabricating anisotropic corn-like α-Fe2O3/AgAu/polydopamine (PDA) nanospindles with photothermally enhanced antibacterial performance. The AgAu bimetallic nanocrystals sandwiched between the α-Fe2O3 core and the PDA shell are achieved by simultaneous reduction from AgNO3 and HAuCl4 during the oxidative polymerization of dopamine and galvanic replacement reaction between the obtained Ag nanocrystals and Au precursor. The inner nanostructure and atom ratio of AgAu bimetallic nanocrystals can be modulated by varying the experimental parameter, thus further controlling the photothermal activities. In comparison to α-Fe2O3/Au/PDA, Fe2O3/Ag1.7Au0.8/PDA (50 μg/mL) has a better photothermal conversion effect (60.69%). Moreover, the α-Fe2O3/Ag1.7Au0.8/PDA nanospindle exhibits moderate antibacterial activity, which may be derived from Ag+ release. Under application of near-infrared light irradiation, the corn-like α-Fe2O3/Ag1.7Au0.8/PDA nanospindles (50 μg/mL) exhibit a photothermally enhanced sterilization effect against both Escherichia coli and Staphylococcus aureus (99.99%). This “Ag+ release–photothermal” coupling sterilization method provides a simple synergistic antimicrobial therapy strategy, which possesses great promise for future nanomedicine against bacteria in water treatment.
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CHEMISTRY OF MATERIALS
·
2019
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IF 10.159
Jiwu Xin,Junyou Yang,Sihui Li,Abdul Basit,Bingyang Sun,Suwei Li,Qiang Long,Xin Li,Ying Chen,Qinghui Jiang
α-MgAgSb (α-MAS) has recently been discovered to be a promising p-type thermoelectric material owing to its earth-abundant and nontoxic nature. However, there are two main disadvantages hindered the large-scale application of α-MAS, one is the single α-MAS phase prepared by conventional method requires for an extended period of time, and the other is the severely bipolar effect thus poor electric properties. In this scenario, we have presented an effective approach typified by SnTe nanocompositing to significantly enhance the thermoelectric performance of the rapidly microwave-synthesized α-MAS system. Specifically, the pure α-MAS compound was first produced by using a rapidly microwave synthesis. After the initial preparation, high-quality SnTe nanoparticles fabricated by a facile solvothermal method were incorporated into the microwave-synthesized α-MAS matrix. It is deserve to be mentioned that the rapidly microwave synthesis purifies the single α-MAS phase and allows the preparative time to be diminished from over 2 weeks to as little as 5 days. Moreover, the severe bipolar effect of pristine α-MAS has been retarded effectively by the following step typified by compositing SnTe nanoinclusions, leading to a large Seebeck coefficient, thus significantly enhanced a power factor in α-MAS/SnTe-composited system. Concurrently, the lattice thermal conductivity has also been greatly reduced because of the extra phonon scattering because of the multiscale hierarchical architecture (e.g., SnTe nanostructures, high-density stacking faults, and elastic strain fields). Eventually, an enhanced figure of merit ZT of ∼1 at 548 K, which increases by ∼53% compared with pristine α-MAS, has been achieved in the 3 at % SnTe-composited sample. This work impels the potential application of the α-MAS thermoelectric material as a robust candidate for a waste heat recovery below 573 K.
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ChemElectroChem
·
2019
·Vol. 6
·期 17
·4437-4443
·
IF 3.975
Yiqun Du,Shimeng Zhao,Cheng Xu,Wenyang Zhang,Shuming Fan,Pan Li,Huixin Jin,Youjian Zhang,Jianxin Zhang
Graphical Interc-Al-ation for batteries : A high-capacity cathode with porous α-MnSe is designed for rechargeable aluminum batteries. The fundamental reaction mechanism of the α-MnSe cathode is deeply researched in the manuscript. The synergistic effect of nanostructure and porosity could efficiently mitigate volume changes of the α-MnSe and enhance intercalation kinetics of Al 3+ , which sheds new light on the establishment of advanced energy-storage devices. Aluminum batteries (ABs) have been considered as a viable candidate for new-generation energy-storage devices due to its low cost and high theoretical volumetric capacity. Despite these advantages, the large-scale application of ABs is constrained by scarce options of suitable cathode materials. Herein, three-dimensional nanostructured α-MnSe microspheres with porous properties are reported as a cathode for ABs. The nanosized and porous structure of α-MnSe could offer numerous open channels and the short ionic transport path, which would efficiently mitigate volume changes and enhance electrochemical reaction kinetics. Moreover, the pseudocapacitive characteristic of Al 3+ storage in α-MnSe contributes to the fast kinetics of the cathode. It is demonstrated that the reversible Al 3+ insertion/extraction occurs in the α-MnSe cathode during the cycling process. The resulting aluminum battery based on the α-MnSe cathode, AlCl 3 /[EMIm]Cl ionic liquid electrolyte, and aluminum anode exhibits an ultrahigh reversible capacity of 408 mA h g −1 at 0.2 A g −1 . Even for a current density at 1 A g −1 , a discharge capacity of 131 mA h g −1 could be retained with a Coulombic efficiency of 97 % over 150 cycles. This strategy has referential significance in aspects of the selection of compatible cathode for ABs.
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高影响因子
Food Bioscience
·
2017
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IF 1.964
Zicong Zeng,Shunjing Luo,Chengmei Liu,Xiuting Hu,Ersheng Gong,Jinyu Miao
Based on the results of phenolic retention of brown rice treated by extrusion with thermostable α-amylase (ETA), this work investigated the phenolic retention of brown rice after extrusion with mesophilic α-amylase (EMA). Similarly, EMA significantly increased phenolic retention of brown rice with the increasing enzyme level, and the phenolic retention was positively correlated with reducing sugars. However, brown rice treated by EMA had higher retention rate of free phenolic content, free antioxidant activity , and total identified free phenolic acids than that treated by ETA at different enzyme levels, although the corresponding reducing sugars produced by EMA were lower than by ETA. These results indicated that the extrusion temperature had greater effect on the phenolic retention than enzymatic hydrolysis during enzymatic extrusion. In addition, brown rice treated by EMA with 0.5% enzyme had a high retention of free flavonoids at 72.4%. Therefore, EMA might be a better processing technology for brown rice to improve its nutrition .
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危险品化学品经营许可证（带存储）