JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Zhurong Mo,Shaoshan Su,Yanping Huo,Hongli Wen,Andrzej Suchocki,Deshmukh Abdul Hakeem
Fundamental understanding of the Stokes and anti-Stokes emissions in luminescence materials and their mechanism is very important for different technological applications. In this article, we report the dual mode, down/upconversion (DC/UC), luminescence in a LiAlSi 2 O 6 (β-spodumene) single host along with its mechanistic investigations. A series of emission tunable Li 1- x AlSi 2 O 6 : x Er 3+ (LAS: x Er 3+ ; 0 ≤ x ≤ 0.07) and Li 0.99- y AlSi 2 O 6 :0.01Er 3+ , y Yb 3+ (LAS:0.01Er 3+ ,yYb 3+ ; 0.001 ≤ y ≤ 0.007) phosphor powders were prepared by solid state reaction method at 1300 °C. The formation of single-phase phosphors is verified by XRD technique and other structural properties are investigated by FT-IR, UV-Vis-NIR spectra. The phosphors produced NIR DC emission at 1535 nm (under 380 and 980 nm excitation) and an intense red UC emission at 655 nm (under both 980 and 808 nm excitations). The dual mode emission intensity of the LAS:0.01Er 3+ is further improved by co-doping Yb 3+ ions via energy transfer from Yb 3+ to Er 3+ . The red UC emission intensity of LAS:0.01Er 3+ ,0.005Yb 3+ phosphor under 980 nm excitation is ~10 times stronger than the singly doped LAS:0.01Er 3+ phosphor. In addition, the phosphors can also be efficiently excited by 808 nm laser and hence can be used for biological application since 808 nm NIR irradiation exhibits higher light penetration depth without inducing overheating. The mechanism of energy transfer from Yb 3+ to Er 3+ by considering different emission pathways viz: ground state absorption (GSA), excited state absorption (ESA), energy transfer upconversion (ETU), back energy transfer (BET) and cross relaxation (CR) are discussed in detail. Based on the dual mode emissions, the LAS: x Er 3+ and LAS: x Er 3+ , y Yb 3+ ( x = 0.1 and 0.001 ≤ y ≤ 0.007) phosphors could be a potential candidate not only for the biological application as bio-probe but also as energy convertors for solid-state lighting, displays, and solar cell applications.
研究领域:
材料科学
·
光学
·
光子学
·
生物技术
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yi-Min Shan,Li-Na Zhang,Si-Xian Wu,Xiao-Liang Li,Hong Zhang,Fei-Fan Xiang,Fan-Yuan Yu,Yan-Hong Liu,Xiao-Qi Yu,Kun Li,Wen-Ming Xu,Kang-Kang Yu
β -galactosidase has become the most widely used bio-marker in cellular senescence, however, it is unknown whether there are same changes in β -galactosidase activity during sperm senescence. Understanding the changes in such indicator during sperm senescence is important for sperm sorting and in vitro fertilization. Herein, we developed a NIR probe Si-βGal-P by using silicon xanthene as fluorophore . Si-βGal-P showed a good affinity to β -galactosidase together with high specificity and strong anti-interference ability. And Si-βGal-P was proved to be able to assess β -galactosidase in three different replicate senescence models. Besides, Si-βGal-P could easily pass blood-brain barrier and successfully imaged the up-regulated β -galactosidase activity in 34-week-old mouse brain. In addition, Si-βGal-P was successfully applied to hydrogen peroxide-induced premature sperm and visualized the up-regulated β -galactosidase activity for the first time, providing sperm senescence with a valid labeling tool.
研究领域:
细胞衰老
·
生殖生物学
·
分子成像
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jingming Wang,Bin Wu,Na Zhang,Mingyue Zhao,Tingting Jing,Yi Wu,YunQing Hu,Feng Yu,Xiaochun Wan,Wilfried Schwab,Chuankui Song
β-Ionone is a carotenoid-derived flavor and fragrance compound with a complex fruity and woody scent, known for its violet aroma. Due to the low odor threshold, β-ionone dramatically affects the aroma and quality of tea. Previous studies have shown that β-ionone increases during tea withering; however, its formation and regulation during the withering process are far from being understood. As dehydration is the most important stress during the withering of the tea leaves, we isolated a dehydration-induced gene belonging to the subfamily of carotenoid cleavage dioxygenases called carotenoid cleavage dioxygenase 1a (CsCCD1a) from Camellia sinensis and expressed it in Escherichia coli. The recombinant protein could generate volatile β-ionone and pseudoionone from carotenoids. CsCCD1a was induced by dehydration stress, and its expression was related to the β-ionone accumulation during tea withering. Overall, this study elucidated that CsCCD1a catalyzes the formation of β-ionone in C. sinensis and enhanced the understanding of the β-ionone formation under multiple stresses during the processing of tea.
研究领域:
植物生物化学
·
农学
·
食品科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Applied Food Research
·
2025
·
IF 6.2
Wang-Wei Zhang,Rui-Xi Zhang,Xiu-Xiu Zhang,Zhi-Jing Ni,Kiran Thakur,Jian-Guo Zhang,Zhao-Jun Wei
This study successfully developed a ternary ZCP-PCPP nanocomposite using zein (ZN), chitosan (CS), and the polyphenols extracted from Polygonatum cyrtonema Hua (PCPP) to stabilize Pickering emulsions. The optimal ZN-CS was 2:1, which resulted in the ZCP-PCPP nanocomposite with the strongest encapsulation ability for PCPP, uniform particle size, and strong structural stability. Infrared spectroscopy, fluorescence spectroscopy, and X-ray diffraction confirmed that ZN, CS, and PCPP interacted mainly through hydrogen bonding and hydrophobic forces. Stability analyses demonstrated that ZCP-PCPP markedly enhanced the physical stability and oxidative resistance of the emulsions. Furthermore, ZCP-PCPP nanoparticles at 3–5% (w/v) effectively protected β-carotene against thermal and ultraviolet degradation. These findings highlight that combining natural polymers with bioactive polyphenols provides an efficient strategy for producing stable Pickering emulsions with strong antioxidant protection potential.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2011
·Vol. 22
·期 2
·729-736
·
IF 1.966
Li Yan-Yan,Yan Bing,Guo Lei
In this paper, the organic ligands TTA and TAA are grafted onto the coupling agent to achieve the organic precursors (TTA-Si, TAA-Si) as first ligand and the organic 4-(4-nitrostyryl)pyridine (Nspy) is synthesized as the second ligand. Both of them are coordinated to the rare ions with the carbonyl group and nitrogen atom respectively. After the hydrolysis and copolycondensation between the teraethoxysilane (TEOS) and the ternary rare earth organic complex via the sol–gel process, the chemical bonded hybrid materials are constructed and characterized in detail. The obtained hybrid materials present superior thermal stabilities and regular and homogenous square blocks microstructure. Among the hybrids Eu(TTA-Si) 3 Nspy shows a more strong characteristic emission and longer lifetime than the hybrids Eu(TAA-Si) 3 Nspy, while hybrids Tb(TAA-Si) 3 Nspy exhibits the stronger characteristic emission and longer lifetime than the hybrids Tb(TTA-Si) 3 Nspy. For Sm 3+ hybrid materials, the photoluminescence of Sm(TAA-Si) 3 Nspy is too weak to find in the characteristic emission spectra, meanwhile, Sm(TTA-Si) 3 Nspy has the excellent luminescent intensity.
研究领域:
材料科学
·
光物理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Lingyu Wei,Libing Chu,Jianlong Wang,Qi Yang
In this study, to explore the influence of protein on antibiotics degradation during ionizing irradiation, the binding interaction between bovine serum albumin (BSA) and the broad spectrum β-lactam and tetractycline antibiotics and its effect on antibiotic degradation were investigated. Static quenching happened between BSA and antibiotics involving penicillin G (PEG), cephalosporin C (CPC), oxytetracycline (OTC) and tetracycline hydrochloride (TTC), indicating the formation of non-fluorescence complexes. The binding capacity followed the order: TTC > CPC > OTC > PEG. As exposed to γ-irradiation, the β-lactam antibiotics showed a higher degradation rate than the tetracyclines. The degradation rate constant ( k ) of CPC and PEG was 1.4–2.0 times higher than that of OTC and TTC. In presence of BSA, the k values ( k BSA ) of all the four antibiotics decreased greatly and the degradation rate of CPC and PEG was still higher by 1.2–1.3 times than that of OTC and TTC. No correlation between k / k BSA and the binding affinity was observed. The presence of BSA has little effect on the trend of abatement of antimicrobial activity to S. aureus during γ-irradiation. This suggests that the inhibition of protein to antibiotic degradation was mainly attributed to the competition of protein for the active species such as ·OH radical formed during γ-irradiation, while the binding ability of protein to antibiotics has no obvious effect. The results of this study contributed to develop technical strategies to improve the removal of antibiotics completely in real water matrices.
研究领域:
环境科学
·
化学
·
药学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2017
·
IF 2.24
Fei Chang,Saisai Xue,Xiaqing Xie,Wei Fang,Zemin Fang,Yazhong Xiao
Complicated purification steps, together with the fact that β-glucosidase has to be tolerant to ethanol restricts the application of β-glucosidase in isoflavone aglycone hydrolyzing process. β-Glucosidase Bgl1A(A24S/F297Y) is a promising enzyme in hydrolyzing isoflavones. In this work, six different carbohydrate-binding modules (CBMs), which were from 3 families, were fused to the C-terminal of Bgl1A(A24S/F297Y), respectively, to simplify the enzyme preparation process. The fusion proteins were expressed in Escherichia coli and adsorbed onto cellulose . The Bgl-CBM 24 was found to have the highest immobilization efficiency at room temperature within 1 h adsorption. Notably, 1-g cellulose absorbs up to 254.9 ± 5.7 U of Bgl-CBM 24 . Interestingly, the immobilized Bgl-CBM 24 showed improved ethanol tolerance ability, with the IC 50 of 35% (v/v) ethanol. Bgl-CBM 24 effectively hydrolyze soybean isoflavone glycosides . The hydrolysis rate of daidzin and gemistin was 85.22 ± 3.24% and 82.14 ± 3.82% within 10 min, with the concentrations of daidzein and genistein increased by 6.36 ± 0.18 mM and 3.98 ± 0.22 mM, respectively. In the repetitive hydrolytic cycles, the concentrations of daidzein and genistein still increased by 3.07 ± 0.24 mM and 1.94 ± 0.34 mM in the fourth cycle with 20% (v/v) ethanol. These results suggest that the immobilized Bgl-CBM 24 has excellent potential in the preparation of isoflavone aglycones.
研究领域:
生物化学
·
酶工程
·
食品科学
✓ 已验证
DOI
高影响因子
Materials Research Express
·
2022
·Vol. 9
·期 2
·025305
·
IF 2.025
Long-juan Ma,Yi-heng Lu,Yan Chen,Yu-wei Lu,Gang Yuan
Understanding the influence of additives on the dehydrochlorination of plasticized poly(vinyl chloride) (PVC) from the source and long-term storage is an attractive topic. In this work, the effects of folic acid modified nano titanium dioxide (FANT), cerium stearate, organotin and β -diketone compound additives on the dehydrochlorination of plasticized PVC and long-term storage were investigated using conductivity measurements. The results showed that the induction time of hydrogen chloride removal of plasticized PVC (C-1) containing composite stabilizer at 200 °C were 243% higher than those of the control (S1). After storage, the heat stability of the control decreased significantly. Instead, the induction time and stability time of plasticized PVC containing FANT, cerium stearate, organotin and β- diketone composites were significantly enhanced at 200 °C. The thermal stability sequence from difficult to easy for removal of hydrogen chloride was : 3.0 phr (1.90% wt) (C2) >1.0 phr (0.64% wt) (C1) > 5.0 phr (3.17% wt) (C3) with FANT. It is inferred that this is due to the FANT's hydrogen bonding van der Waals force and the complexation reaction of variable valence metal Ce+3/Ce+4 with C-Cl bond in PVC polymer molecule. The induction time (min) of hydrogen chloride removal at 200 °C before and after storage is as follows: C-20 (71.9) > C-10 (70.6) > C-1 (60.7) > C-3 (57.2) > C-30 (52.7) > C-2 (49.9) > S1 (25.3) > S10 (19.7). The critical temperature for hydrogen chloride removal by the composite film is around 210 °C. If the content of FANT increased to 3.17%, the intermolecular force and repulsive force in PVC system offset each other, indicating that the increase of FANT content will lead to the increase of repulsive force with PVC. A certain amount of FANT, cerium stearate, organotin and β- diketone composites has a significant synergistic effect on the thermal stability of PVC.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
Journal of the Mechanical Behavior of Biomedical Materials
·
2019
·
IF 3.485
Tao Feng,Junjie Niu,Bin Pi,Yingjie Lu,Jinning Wang,Wen Zhang,Bin Li,Huilin Yang,Xuesong Zhu
High brittleness and lack osteogenesis are two major limitations of calcium phosphate cement (CPC) in application in bone defect reconstruction. Here we prepared a composite calcium phosphate cement by mixing N-acetyl cysteine loaded silk fibroin solution with α-tricalcium phosphate. In vitro cytology experiment revealed that SF-NAC/α-TCP could significantly increase the activity of exocrine ALP and up-regulated expression of bone-related genes. However, NAC up-regulated gene expression could be significantly suppressed by DKK1. We propose that NAC functioning as osteogenic factor by activating the Wnt/β-catenin signaling pathway may be the possible mechanism of up-regulation of osteogenic genes. Bone regeneration in vivo shown in a rat femur defect was enhanced by the addition of NAC in SF/α-TCP. In addition, the combination intensity of cement-bone interface was improved. The combination SF-NAC/α-TCP might be developed into a promising tool for bone tissue repair in the clinic.
研究领域:
生物材料学
·
骨组织工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2016
·Vol. 45
·期 9
·3853-3862
·
IF 4.177
Ao Wang,Weiwei Jin,Enyi Chen,Jiahong Zhou,Lin Zhou,Shaohua Wei
Near-infrared (NIR) light triggered photodynamic therapy (PDT), based on upconversion nanoparticles (UCNPs), has attracted great attention because of its high tissue penetration and low photodamage to living organisms. However, most UCNPs cannot be stably dispersed in aqueous solution and cannot carry photosensitive drugs directly. Besides, UCNP mediated PDT is a fluorescence resonance energy transfer (FRET) process from the UCNPs to the attached photosensitive drugs. So the drug and UCNPs must be stably connected and close enough. In this manuscript, carboxymethyl-β-cyclodextrin (COOH-β-CD) was used to connect UCNPs and adamantine modified phthalocyanine (Ad-ZnPc) through a self-assembly process followed by a host–guest interaction. COOH-β-CD can provide good water solubility of the system and short-distance linking between the UCNPs and Ad-ZnPc. Most importantly, the system has a strong NIR light triggered PDT activity toward cancer cells.
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
·
药学科学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Rui Hao,Tian Li,Beibei Zhou,Jiaxin Shao,Weibo Zhao,Beibei Jiao,Xia Sun,Jian Zhang
Background : Bushen Huoxue Formula (BSHXF), a traditional Chinese medicine, effectively treats premature ovarian insufficiency (POI) by modulating of lipid metabolism. Purpose : This study aimed to investigate the underlying mechanism through which BSHXF improves POI by regulating lipid metabolism. Methods Network pharmacology identified potential pathways associated with BSHXF in POI. A POI rat model was induced using subcutaneous corticosterone injections and treated with BSHXF to assess its efficacy. The impact of BSHXF on lipid metabolism, autophagy, and the LRP6/β-catenin signaling pathway was evaluated in these rats. Additionally, palmitic acid (PA)-induced granulosa cells (KGN cells) were used in vitro . A LRP6 inhibitor (Salinomycin sodium salt, Sal) was applied to these cells, followed by a series of molecular biology experiments to elucidate the specific regulatory role of BSHXF on lipid metabolism. Results : Network pharmacology analysis indicated that the Wnt/β-catenin signaling pathway is a potential target of BSHXF in POI. In vivo , BSHXF alleviated POI-like symptoms in rats, reduced lipid accumulation, suppressed excessive autophagy, and activated the LRP6/β-catenin signaling pathway. In vitro , Sal inhibited the ability of BSHXF-containing serum to activate LRP6/β-catenin signaling, reduce lipid accumulation, and counteract excessive autophagy in PA-induced KGN cells. This was accompanied by increased expression of LRP6 and β-catenin, enhanced lipogenesis, elevated lipid droplet formation, decreased lipid catabolism, and oxidation, and enhanced autophagic flux. Cycloheximide chase and Western blot analyses further confirmed that BSHXF stabilizes LRP6 by inhibiting its ubiquitin-proteasomal degradation. Conclusions : BSHXF activates LRP6/β-catenin signaling pathway by inhibiting the ubiquitination degradation of LRP6, thereby improving POI. This study reveals that BSHXF may improve POI by modulating LRP6 stability, thereby affecting lipid metabolism.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Jun Zhang,Ze-hua Liu,Shu-shu Zhong,Hao Wang,Bamu Caidan,Hua Yin,Zhi Dang
Arylsulfatase and β-glucuronidase are two important enzymes that are responsible for deconjugation of estrogen conjugates. It is important to keep estrogen conjugates intact during sample collection and storage, while the effective inhibition conditions for arylsulfatase and β-glucuronidase remain unknown. To elucidate these conditions, inhibition experiments were performed by adding several inhibitors or by introducing extreme pH conditions. This work confirms that arylsulfatase and β-glucuronidase can tolerate some extremes, including high concentrations of mercury dichloride , ethanol, and EDTA, while low pH (<3) or high pH (>11) can effectively inhibit their activities. The high tolerance of arylsulfatase and β-glucuronidase for mercury dichloride explains why estrogen conjugates in wastewater samples were deconjugated, even in the extremely unfavorable condition with a high concentration of mercury dichloride. Although low pH (<3) can effectively inhibit arylsulfatase/β-glucuronidase, deconjugation of sulfate conjugates by acid hydrolysis readily occurs; thus, a high pH of 11 is an appropriate storage condition for the effective inhibition of arylsulfatase/β-glucuronidase. This appropriate storage condition was confirmed and validated with diluted and sterilized activated sludge samples in which arylsulfatase/β-glucuronidase inhibition was effective for 48 h at room temperature and with a high pH of 11. The developed appropriate storage condition for effective inhibition of arylsulfatase/β-glucuronidase has wide application potential not only for estrogen conjugates but also for all conjugates of other organic micropollutants .
研究领域:
环境科学
·
生物化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）