Food Analytical Methods
·
2016
·Vol. 10
·期 1
·111-117
·
IF 2.167
Liu Qi,Wang Li,Hu Jie,Miao Yanni,Wu Zixue,Li Jianrong
A rapid and sensitive analytical method for the determination of main organic acids in wines was developed by capillary electrophoresis (CE) with indirect UV detection. Separation parameters including the pH of electrolyte, chromophore, and its concentration were optimized. The results showed that the optimal electrolyte for the separation of organic acids consisted of 10 mmol L −1 2, 4-dihydroxybenzoic acid (DHBA) at pH 3.6 containing 0.4 mmol L −1 cetyltrimethlammonium bromide (CTAB). DHBA as chromphore was selected based on the close mobility to the analyte and relatively large molar extinction coefficient. The method showed good linearity with limits of detection (LOD), ranging from 0.5 to 7.5 mg L −1 . The relative standard deviation (RSD) of interday precisions for the peak area and the migration time were less than 7.2 and 0.8 %, respectively. The recoveries of the analyte in Chinese rice wine sample were between 90.3 and 106.9 % with RSD ranging from 1.4 to 8.9 %. The method was successfully applied to the commercial rice wine and beer samples with dilution and filtration.
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BMC GENOMICS
·
2017
·Vol. 18
·期 1
·1-15
·
IF 3.729
Lu Yuzhen,Luo Feifei,Cen Kai,Xiao Guohua,Yin Ying,Li Chunru,Li Zengzhi,Zhan Shuai,Zhang Huizhan,Wang Chengshu
Background Ascomycete Cordyceps species have been using as valued traditional Chinese medicines. Particularly, the fruiting bodies of Cordyceps cicadae (syn. Isaria cicadae ) have long been utilized for the treatment of chronic kidney disease. However, the genetics and bioactive chemicals in this fungus have been largely unexplored. Results In this study, we performed comprehensive omics analyses of C. cicadae , and found that, in contrast to other Cordyceps fungi, C. cicadae produces asexual fruiting bodies with the production of conidial spores instead of the meiotic ascospores. Genome sequencing and comparative genomic analysis indicate that the protein families encoded by C. cicadae are typical of entomopathogenic fungi, including the expansion of proteases and chitinases for targeting insect hosts. Interestingly, we found that the MAT1-2 mating-type locus of the sequenced strain contains an abnormally truncated MAT1-1-1 gene. Gene deletions revealed that asexual fruiting of C. cicadae is independent of the MAT locus control. RNA-seq transcriptome data also indicate that, compared to growth in a liquid culture, the putative genes involved in mating and meiosis processes were not up-regulated during fungal fruiting, further supporting asexual reproduction in this fungus. The genome of C. cicadae encodes an array of conservative and divergent gene clusters for secondary metabolisms. Based on our analysis, the production of known carcinogenic metabolites by this fungus could be potentially precluded. However, the confirmed production of oosporein raises health concerns about the frequent consumption of fungal fruiting bodies. Conclusions The results of this study expand our knowledge of fungal genetics that asexual fruiting can occur independent of the MAT locus control. The obtained genomic and metabolomic data will benefit future investigations of this fungus for medicinal uses.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2016
·
IF 1.207
Hao Sun,Saijian Shi,Zhenggui Gu
A preparation process of 2-methylnaphthalene (2-MN) was proposed by isomerization , side-stream distillation and extractive distillation. The isomerization of alkyl naphthalene was catalyzed by acid-treated HBEA zeolites, and the 2-MN selectivity of isomerization was 92.70%. Side-stream distillation and extractive distillation were investigated by simulation, and effects of operation parameters on 2-MN were studied. Further, the simulated results were verified by experiment. Under the optimal condition, the mass fraction of 2-MN reached to 98.09% in the product, and the yield was 83.84% in refining process.
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BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Xue-Dan Hou,Ao-Lin Li,Kai-Peng Lin,Yuan-Yuan Wang,Zhi-Yin Kuang,Shi-Lin Cao
Rice straw pretreatment mediated by choline chloride (ChCl) or lactic acid (Lac) sequences deep eutectic solvents (DESs) was investigated in this work. Hydrogen bond acceptors (HBAs) and hydrogen bond donors (HBDs) proved to be both important for DESs pretreatment efficiency. DESs containing lots of hydroxyl or amino groups with a high intermolecular hydrogen-bond (H-bond) strength exhibited weak biomass deconstruction abilities. The presence of strong electron-withdrawing groups in DESs was benefit for xylan removal, thus furnishing higher cellulose digestibility. The relationships between the properties of DESs, xylan removal and cellulose digestibility of pretreated biomass were established. It was found that xylan removal was negatively correlated with the pKa values of HBDs, and the enzymatic cellulose digestibility of the residues was linearly and positively related to xylan removal instead of delignification. These results provide a preliminary reference for rational design of novel DESs for biomass pretreatment.
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MOLECULES
·
2017
·Vol. 22
·期 7
·1068
·
IF 2.861
Yalu Dong,Panpan Peng,Baoping Hu,Hui Su,Shenghua Li,Siping Pang
High-energy metal–organic frameworks (MOFs) based on nitrogen-rich ligands are an emerging class of explosives, and density is one of the positive factors that can influence the performance of energetic materials. Thus, it is important to design and synthesize high-density energetic MOFs. In the present work, hydrothermal reactions of Cu(II) with the rigid polynitro heterocyclic ligands 5,5′-dinitro-2H,2′H-3,3′-bi-1,2,4-triazole (DNBT) and 5,5′-dinitro-3,3′-bis-1,2,4-triazole-1-diol (DNBTO) gave two high-density MOFs: [Cu(DNBT)(ATRZ)3]n(1) and [Cu(DNBTO)(ATRZ)2(H2O)2]n(2), where ATRZ represents 4,4′-azo-1,2,4-triazole. The structures were characterized by infrared spectroscopy, elemental analysis, ultraviolet-visible (UV) absorption spectroscopy and single-crystal X-ray diffraction. Their thermal stabilities were also determined by thermogravimetric/differential scanning calorimetry analysis (TG/DSC). The results revealed that complex1has a two-dimensional porous framework that possesses the most stable chair conformations (like cyclohexane), whereas complex2has a one-dimensional polymeric structure. Compared with previously reported MOFs based on copper ions, the complexes have higher density (ρ = 1.93 g cm−3for complex1and ρ = 1.96 g cm−3for complex2) and high thermal stability (decomposition temperatures of 323 °C for complex1and 333.3 °C for complex2), especially because of the introduction of an N–O bond in complex2. We anticipate that these two complexes would be potential high-energy density materials.Keywords:energetic materials;metal organic frameworks;densities;polynitro heterocyclic ligands
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EUROPEAN JOURNAL OF MASS SPECTROMETRY
·
2017
·
IF 1.022
Dengyang Yin,Xunxiu Hu,Dantong Liu,Wencheng Du,Haibo Wang,Mengzhe Guo,Daoquan Tang
Liquid chromatography coupled with mass spectrometry technique has been widely used in the analysis of biological targets such as amino acids, peptides, and proteins. In this work, eight common single carboxylic acids or diacids, which contain different pKa have been investigated as the additives to the analysis of amino acids. As the results, carboxylic acid additive can improve the signal intensity of acidity amino acids such as Asp and Glu and the chromatographic separation of basic amino acids such as Arg, His, and Lys. In particular, the diacids have better performance than single acids. The proposed mechanism is that the diacid has hydrogen bond interaction with amino acids to reduce their polarity/amphiprotic characteristics. Besides, oxalic acid has been found having better enhancement than phthalic acid by overall consideration. Therefore, we successfully quantified the 15 amino acids in Sepia bulk pharmaceutical chemical by using oxalic acid as the additive.
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Xiao Shang,Kai-Li Yan,Zi-Zhang Liu,Shan-Shan Lu,Bin Dong,Jing-Qi Chi,Xiao Li,Yan-Ru Liu,Yong-Ming Chai,Chen-Guang Liu
Oxidized carbon fiber (oCF) as support successfully realizes the vertical growth of uniform WS 2 nanosheets arrays for efficient hydrogen evolution reaction (HER) via a facile hydrothermal process. Thanks to oxygen functional groups on oCF, vertical WS 2 nanosheets structures have grown more easily on oCF, which can provide better dispersion, short charge transfer distance and more exposed active sites for HER in comparison with bulk WS 2 and WS 2 nanosheets on bare carbon fiber (CF) fabricated at the same condition. The electrochemical measurements confirmed that WS 2 /oCF possesses better HER activity than bulk WS 2 and WS 2 /CF. Especially, the 10-h stability with unchanged vertical WS 2 nanosheets morphology further demonstrate the positive effect of oxygen functional groups on the enhanced vertical structure and close combination between WS 2 and oCF. It may offer a facile way to realize more exposed active sites from stable electrocatalyst hybrids of transition metal sulfides by surface oxidization of carbon supports.
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WATER AIR AND SOIL POLLUTION
·
2016
·Vol. 227
·期 9
·1-11
·
IF 1.551
Wu Hailu,Xu Kaiqin,He Xiaojuan,Wang Xinze
Three macrophyte species, Typha augustifolia ( T. augustifolia ), Phragmites australis ( P. australis ), and Acorus calamus L. ( A. calamus L.), have been grown in hydroponic cultivation systems fed with synthetic wastewater. The experiment was designed as 3 × 2 factorial, with three species and two ratios of NH 4
+ /NO 3
− so as to investigate the nitrogen transformation and nitrogen removal capacity of each species. The nitrogen removal mechanism was further disclosed by comparing biomass production, nitrogen mass balance, and root exudates of the three plant species under different NH 4
+ /NO 3
− ratios. The results indicated there exists a linear relationship, with positive significance ( r = 0.946, p < 0.05), between plant biomass and total nitrogen (TN) removal efficiency; in other words, biomass could best reflect plant ability to remove nitrogen. It is also found that NH 4
+ /NO 3
− ratio could influence plant biomass and root exudates significantly. Additionally, the hydrogen donor and source of energy in denitrification happened in this research were mainly organic acids and soluble sugars, accounting for approximately 50 % of the composition in root exudates.
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Nanoscale
·
2016
·Vol. 8
·期 47
·19779-19786
·
IF 7.76
Mingyu Pi,Tianli Wu,Dingke Zhang,Shijian Chen,Shuxia Wang
The design and development of high-efficiency and non-noble metal hydrogen evolution reaction (HER) electrocatalysts with optimized nanostructures for human clean and sustainable energy systems has attracted significant research interest over the past years. Herein, self-supported semimetallic tungsten diphosphide nanowire arrays on carbon cloth (WP2 NWs/CC) were topotactically fabricated by in situ phosphidation of a WO3 NWs/CC precursor. Such a binder-free flexible HER cathode with integrated three-dimensional nanostructures can not only provide a large surface area to expose abundant active sites, but also facilitate electrolyte penetration for electrons and electrolyte ions. The WP2 NWs/CC electrode exhibits superior catalytic performance, and it needs overpotentials of 109 and 160 mV with a small Tafel slope of 56 mV dec−1 to achieve current densities of 10 and 50 mA cm−2, respectively. High stability in acidic media is also observed for the catalyst for a duration of 20 hours at least. In addition, density functional theory (DFT) calculations indicate a low kinetic energy barrier for H atom adsorption on the WP2 surface which guarantees the excellent catalytic activity of the catalyst, and the influences of phosphidation temperature on the HER activity are also studied. The excellent electrocatalytic activity makes the present 3D structured WP2 NWs/CC a promising catalyst for large scale highly pure hydrogen evolution by electrochemical water splitting.
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RARE METALS
·
2025
·
IF 11
Hou Yao-Lin,Zhang Yu-Qing,Li Dan,Xie Hai-Ming,Liu Jia,Liu Yu-Long,Zhu Jie-Fang
Developing effective heterostructure strategies to mitigate the shuttling effect and accelerate lithium polysulfide (LiPS) conversion remains a critical challenge in lithium–sulfur (Li–S) batteries. Here, we report the first carbon–free VO 2 –VS 2 heterostructure material synthesized via in situ sulfurization, applied as a modifier on a commercial polypropylene (PP) separator (denoted as VO 2 –VS 2 @PP). The as–prepared VO 2 –VS 2 nanorods synergistically combine the high absorptivity of VO 2 with the efficient catalytic properties of VS 2 , simultaneously enhancing LiPS anchoring and promoting its conversion. We systematically investigate the influence of material composition on battery performance, leveraging these functional attributes, Li–S cells incorporating VO 2 –VS 2 @PP exhibit exceptional cycle stability (over 500 cycles at 1C), impressive rate performance (807 mAh·g –1 at 5C), desirable reversibility (49.9% capacity retention after 300 cycles at 5C) and exceptional pouch cell performance (3.65 mAh·cm –2 after 50 stable cycles at 0.1C). This study underscores the potential of tailored heterostructures in realizing high–performance Li–S batteries, offering new insights for next–generation energy storage solutions. Graphical abstract
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Food Bioscience
·
2025
·
IF 5.9
Chunyun Qu,Jianjun Li,Fuxin Liu,Yu Zeng,Yiyin Xu,Weidong Bai,Hao Dong,Gongliang Liu
To diversify the aromas and enhance the quality of fruit wines, Zygosaccharomyces mellis LGL-1 (LGL-1), as a non-conventional yeast, was applied for fruit winemaking along with biochar facilitating the fermentation. Results demonstrated that LGL-1 exhibited higher synthesis of ethanol and esters compared to the commercial winemaking yeast CV171 during wampee/grape/litchi winemaking. Intriguingly, the main esters produced by LGL-1 were quite different from those of CV171, resulting in diverse flavors. Four kinds of food-grade biochar were applied to facilitate the fermentation of LGL-1 under the mimic solution of fruit juice (MSM medium) respectively, and results showed that the coconut shell granule biochar (G2) was the best choice with both substrate consumption and product formation improved significantly, especially with esters enhanced by 357.87%, owing to the reduced oxidation-reduction potential, improved intracellular ratio of NADH/NAD + and enhanced activities of esterase. When applied for wampee/grape/litchi winemaking, G2 could also facilitate the fermentation of LGL-1 with total content of esters in each wine improved by 173.7%, 71.8% and 44%. The total concentration of higher alcohols in wampee/grape wine was enhanced by 35.6% and 21.1%, but unchanged significantly in litchi wine. The content of total organic acids in grape wine and litchi wine was changed insignificantly but decreased by 16.7% in wampee wine, which might not affect the flavor based on the taste threshold of organic acids. Data above indicated that LGL-1 is a potential candidate for fruit winemaking, and the fermentation fortified by G2 is a promising method for improved aromas of fruit wine.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-16
·
IF 10.6
Li Qian,Liu Chenguang,Xing Dongming
On-demand dissolution of hydrogels has shown much potential in easy and pain-free removal of wound dressings. This work firstly describes a type of carbon dots (CDs) for dissolving Ca-alginate hydrogel via site-specific mineralization method. The CDs were characterized by two features, which included presence of primary/secondary amine groups and generation of calcium crystals with Ca 2+ . Especially, the amount of primary/secondary amine groups on CDs played key role in determining whether hydrogel could be dissolved. When there were sufficient primary/secondary amine groups, the mineralization occurred on CDs rather than alginates due to the hydrogen bond between primary/secondary amine and carboxyl of alginates. Thereby, this promoted the gel-sol transition through Ca 2+ capture from the hydrogels. Moreover, antibacterial test revealed Ca 2+ capture from cell walls, while in vivo test revealed hypoxia relief due to porous structures of the renewed hydrogels. Overall, CDs with sufficient primary/secondary amine groups could dissolve Ca-alginate hydrogel through site-specific mineralization method, accompanying by additional functions of antibacterial and hypoxia relief. Graphical Abstract
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