JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Yang Yuan,Jiaxin Wang,Xinjiong Ni,Yuhua Cao
A biosensor for determination of 3-chloro-1, 2-propandiol (3-MCPD) was developed based on the electrochemically catalytic reduction by hemoglobin (Hb). Hb was concentrated and immobilized with magnetic molecularly imprinted nanoparticles (MMIPs NPs). The MMIP NPs had a core-shell structure, with magnetic Fe 3 O 4 NPs as the core and SiO 2 as the shell. MMIPs NPs were modified on the surface of a magnetic electrode to constitute the Hb enzyme-like sensor. The biosensor could catalyze the electrochemical reduction of 3-MCPD, and a direct electrochemical determination of 3-MCPD could be achieved for the first time. The biosensor, where Hb immobilized with MMIPs NPs had high electrochemical response for 3-MCPD, increased by 37.5% compared with Hb immobilized with magnetic non-imprinted nanoparticle (MNIPs NPs). The biosensor increased the response by 19.56% again under an external magnetic field . The reduction peak current had a linear relationship with 3-MCPD concentration ranging from 1.0 mg·L −1 to 160 mg·L −1 with a detection limit (S/N = 3) of 0.25 mg·L −1 . The biosensor was applied to the determination of 3-MCPD in soy sauce samples successfully with recoveries between 97.4% and 99.6%.
研究领域:
生物传感器
·
分析化学
·
食品科学
·
电化学
·
分子生物学
·
纳米医学
✓ 已验证
DOI
高影响因子
Current Protocols
·
2023
·Vol. 3
·期 11
·e923
·
IF 0
Xuan Chen,Long Xie,Chenglong Zhang,Shen Tian,Zeyu Tang,Weiwei Tian,Patrick Lu,Xianbin Yang
Although small interfering RNA (siRNA) is a key player among gene inhibition therapeutics, there are many obstacles to the development of siRNA drugs due to inherent properties of oligonucleotides, including the unsatisfactory stability of unmodified siRNA, poor pharmacokinetic distribution, and the toxicity induced by off-target effects. To maximize treatment potency, chemical modification of siRNA has undoubtedly been the most successful strategy by far. Widely applied modifications include phosphorothioate linkages, 2′- O -methyl modifications, and 2′-fluoro modifications, among others. To extend the family of chemical modifications for oligonucleotides, 2′- O -cyanoethylated RNA analogs were developed through the replacement of the 2′-hydroxyl group with a 2′- O -cyanoethyl group (-OCH 2 CH 2 CN). This modification can provide several advantages over unmodified RNA, such as increased stability, improved binding affinity to complementary DNA or RNA strands, and resistance to degradation by cellular nucleases. The 2′- O -cyanoethyl-modified RNAs not only are applied in RNA silencing machinery but also act as research tools for studying RNA structure and function or for developing RNA-based diagnostics. Therefore, the efficient synthesis, deprotection, purification, and characterization of 2′- O -cyanoethylated RNAs deserves more attention. This protocol describes the chemical synthesis of 2′- O -cyanoethylated nucleotides and the solid-phase synthesis, deprotection, and purification of 2′- O -cyanoethylated RNAs. © 2023 Wiley Periodicals LLC. Basic Protocol 1 : Preparation of 6- N -dimethylformamidyl-5′- O -dimethoxytrityl-2′- O -cyanoethyl adenosine 3′-(2-cyanoethyl N , N -diisopropyl)phosphoramidite Basic Protocol 2 : Preparation of 4- N -acetyl-5′- O -dimethoxytrityl-2′- O -cyanoethyl cytidine 3′-(2-cyanoethyl N , N -diisopropyl)phosphoramidite Basic Protocol 3 : Preparation of 2- N -dimethylformamidyl-5′- O -dimethoxytrityl-2′- O -cyanoethyl guanine 3′-(2-cyanoethyl N , N -diisopropyl)phosphoramidite Basic Protocol 4 : Preparation of 5′- O -dimethoxytrityl-2′- O -2-cyanoethyl uridine 3′-(2-cyanoethyl N , N -diisopropyl)phosphoramidite Basic Protocol 5 : Solid-phase synthesis of 2′- O -cyanoethylated RNA analogs Basic Protocol 6 : Deprotection and purification of synthesized 2′- O -cyanoethyl-RNAs
研究领域:
分子生物学
·
生物化学
·
药物化学
·
RNA治疗学
·
有机合成
✓ 已验证
DOI
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Yuzhu Qian,Xinni Li,Hongrui Wang,Ting Song,Yong Pei,Li Liu,Xianyou Wang,Qi Deng,Xiongwei Wu,Bei Long
Increasing insertion host materials are developed as high-performance anodes of “rocking-chair” zinc ion batteries. However, most of them show unsatisfactory rate capabilities. Herein, layered BiOIO3 is reported as an excellent insertion host and a zinc ion conductor, i.e., Zn3(PO4)2·4H2O (ZPO), is introduced to construct a BiOIO3@ZPO heterojunction with a built-in electric field (BEF). Both ZPO and a BEF obviously enhance Zn2+ transfer and storage, which is proven by theoretical calculations and experimental studies. The conversion-type mechanism of BiOIO3 is revealed through ex situ characterizations. The optimized electrode exhibits a high reversible capacity of 130 mAh g–1 at 0.1 A g–1, a low average discharge voltage of 0.58 V, an ultrahigh rate performance with 68 mAh g–1 at 5 A g–1 (52% of capacity at 0.1 A g–1), and an ultralong cyclic life of 6000 cycles at 5 A g–1. Significantly, the BiOIO3@ZPO//Mn3O4 full cell shows a good cyclic life of 67 mAh g–1 over 1000 cycles at 0.1 A g–1. This work provides a new insight into the design of anodes with excellent rate capability.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
DOI
PubMed
高影响因子
POLYMER
·
2012
·
IF 3.438
Hua Ye,Dongcheng Chen,Ming Liu,Xiao Zhou,Shi-Jian Su,Yong Cao
A series of light-emitting conjugated polymers (LEPs) based on building blocks of electron-donating trifluoren-2-ylamine (TFA), electrically neutral 1,3,5-trifluoren-2-ylbenzene (TFB), and electron-withdrawing 2,4,6-trifluoren-2-yltriazine (TFT) were successfully synthesized via palladium-catalyzed Suzuki cross-coupling polycondensation . Their structure–property relationships were thoroughly studied. For P1 containing electron-withdrawing backbone and electron-donating pendants, their photophysical properties in solution are strongly dependent on the solvent polarity due to the intense intramolecular charge-transfer (ICT) interaction. In addition, their emission colors and energy levels could be effectively tuned by changing the structure of the main chain as well as the substituent at the pendent fluorene of TFT, TFB, and TFA. To evaluate their electroluminescence properties, double-layer devices with a configuration of ITO/PEDOT:PSS (40 nm)/emitting layer (EML) (70–80 nm)/TPBI (30 nm)/CsF (1.5 nm)/Al were fabricated, where the developed polymers were used as an EML and TPBI (2,2′,2″-(1,3,5-benzenetriyl)-tris-(1-phenyl-1H-benzimidazole)) was used as an electron-transport and hole-block layer. Deep-blue light emission was achieved for the device based on P1d , a polymer based on TFT and the second generation (G2) of carbazole dendrimer pendant, that shows a maximum current efficiency (CE) of 1.26 cd A −1 , corresponding to external quantum efficiency (EQE) of 1.27%, with Commission Internationale de L'Eclairage (CIE) coordinates of (0.16, 0.14). In comparison, due to the intense ICT interaction between the main chain and the side chain, light-green emission was achieved for the device based on P1c , a polymer based on TFT and bis(9,9-dioctyl-9H-fluoren-2-yl)amine pendant, giving the highest maximum CE of 4.10 cd A −1 , corresponding to EQE of 2.45%, with CIE coordinates of (0.24,0.51), although either of the main chain and the side chain emits blue light.
研究领域:
材料科学
·
有机电子学
·
聚合物化学
·
光电子学
·
光物理
✓ 已验证
DOI
高影响因子
JOURNAL OF DAIRY SCIENCE
·
2023
·
IF 3.5
Yanmei Xi,Sana Ikram,Tong Zhao,Yiwei Shao,Ruirui Liu,Fuhang Song,Baoguo Sun,Nasi Ai
ABSTRACT Flavor sensation is one of the most prevalent characteristics of food industries and an important consumer preference regulator of dairy products. So far, many volatile compounds have been identified, and their molecular mechanisms conferring overall flavor formation have been reported extensively. However, little is known about the critical flavor compound of a specific sensory experience in terms of oxidized off-flavor perception. Therefore, the present study aimed to compare the variation in sensory qualities and volatile flavors in full-fat UHT milk (FFM) and low-fat UHT milk (LFM) samples under different natural storage conditions (0, 4, 18, 25, 30, or 37°C for 15 and 30 d) and determine the main component causing flavor deterioration in the FFM and LFM samples using sensory evaluation, electronic nose, and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). In addition, the Pearson correlation between the volatile flavor components and oxidative off-flavors was analyzed and validated by sensory reconstitution studies. Compared with the LFM samples, the FFM samples showed a higher degree of quality deterioration with increased storage temperature. Methyl ketones of odd carbon chains (i.e., 2-heptanone, 2-nonanone, 2-undecanone, 2-tridecanone, and 2-pentadecanone) reached a maximum content in the FFM37 samples over 30 d storage. The combined results of the Pearson correlation and sensory recombination study indicated that 2-heptanone, 2-nonanone, and 2-undecanone conferred off-flavor perception. Overall, the present study results provide potential target components for detecting and developing high-quality dairy products and lay a foundation for specific sensory flavor compound exploration in the food industry.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Qingxiang Zhou,Man Lei,Jing Li,Kuifu Zhao,Yongli Liu
Magnetic Fe@MgAl-layered double hydroxides (MgAl-LDHs) composite was firstly synthesized by coating MgAl-layered double hydroxides on the surface of the dispersed nanoscale zero valent irons with co-precipitation method and characterized by transmission electron microscopy and X-ray diffraction techniques. The synthesized Fe@MgAl-LDHs nanoparticles were investigated for magnetic solid phase extraction (MSPE) of 1-naphthol and 2-naphthol from the water samples. The elutent containing 1-naphthol and 2-naphthol was analyzed by high performance liquid chromatography with variable wavelength detection (HPLC–UV). Under optimal conditions, there is good linear relationship between the concentration and the peak area in the range of 0.5–200 μg L −1 with the correlation coefficients ( r 2 ) above 0.998 for 1-naphthol and 2-naphthol. The limits of detection were 0.22 μg L −1 and 0.19 μg L −1 for 1-naphthol and 2-naphthol, respectively, and precisions were both below 2.5% ( n = 6). The real water analysis demonstrated that the spiked recoveries were in the range of 79.2–80.9% ( n = 3). All these results indicated that the developed MSPE–HPLC–UV method was proved to be an efficient tool for the analysis of naphthols.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Yanluo Xie,Huakang Liu,Hao Li,Hao Tang,He Peng,Heng Xu
Di-(2-ethylhexyl) phthalate (DEHP) has been classified as a priority pollutant which increased the healthy risk to human and animals dramatically. Hence, a novel biochemical system combined by DEHP-resistant bacterial flora (B) and a green oxidant of persulfate (PS) activated by Nano-Fe 3 O 4 was applied to degrade DEHP in contaminated soil. In this study, the resistant bacterial flora was screened from activated sludge and immobilized by sodium alginate (SAB). Nano-Fe 3 O 4 was coated on biochar (BC@Fe 3 O 4 ) to prevent agglomerating in soil. X-ray diffraction (XRD) and scanning electron microscope (SEM) were utilized to characterize BC@Fe 3 O 4 . Results demonstrated that the treatment of biochemical system (SAB + BC@Fe 3 O 4 + PS) presented the maximum degradation rate about 92.56% within 24 days of incubation and improved soil microecology. The 16S rDNA sequences analysis of soil microorganisms showed a significantly different abundance and a similar diversity among different treatments. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional genes difference analysis showed that some metabolic pathways, such as metabolism of cofactors and vitamins, energy metabolism, cell growth and death, replication and repair, were associated with the biodegradation of DEHP. Besides, DEHP was converted to MEHP and PA by biodegradation, while DEHP was converted to DBP and PA by persulfate and BC@Fe 3 O 4 , and then ultimately degraded to CO 2 and H 2 O.
研究领域:
环境科学
·
微生物学
·
土壤学
·
基因组学
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DOI
PubMed
高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2021
·Vol. 146
·期 6
·2445-2462
·
IF 4.626
Usman Muhammad,Yu Haojie,Wang Li,Titinchi Salam,Khan Amin,Naveed Kaleem-ur-Rehman,Nazir Ahsan,Elshaarani Tarig,Fahad Shah,Amin Bilal ul
Ferrocene (Fc)-based polymers have promising application in burning rate catalysts (BRCs) for solid composite propellants, and some of them have already been practically used. However, they have some drawbacks such as high migration during a long-time storage. Copolymers of poly(2-(methacryloyloxy) ethyl ferrocene carboxylate- co -glycidyl methacrylic acid)s (P(FHEMA -co- GMA)s) were synthesized to overcome the migration problem of Fc-based BRCs as well as to boost the burning rate (BR) of ammonium perchlorate (AP)-based propellants. The structures of the obtained P(FHEMA -co- GMA)s were confirmed by proton nuclear magnetic resonance ( 1 H NMR) and Fourier transform infrared (FT-IR) spectroscopy. Cyclic voltammetry (CV) method was used to study their electrochemical behavior, and the P(FHEMA -co- GMA)s showed redox behavior for the existence of the Fc units. Because of the formation of integrated network structures, the P(FHEMA -co- GMA)s have good anti-migration performances. The BR catalytic property of P(FHEMA -co- GMA)s were investigated by thermogravimetry (TG) and differential thermogravimetry (DTG). They showed competent catalytic effects on the thermal decomposition of AP.
研究领域:
材料科学
·
电化学
·
推进剂技术
✓ 已验证
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Chenxia Yang,Ying Meng,Xintong Wang,Xin Li,Tong Yu,Weiming Liao,Wenjun Xie,Qianchen Jiang,Han Wang,Cheng Shi,Wenxuan Jiao,Xiling Bian,Fang Hu,Xiaowei Wang,Yani Liu,Liangren Zhang,KeWei Wang,Qi Sun
Enhancing α7 nAChR function serves as a therapeutic strategy for cognitive disorders. Here, we report the synthesis and evaluation of 2-arylamino-thiazole-5-carboxylic acid amide derivatives 6–9 that as positive allosteric modulators (PAMs) activate human α7 nAChR current expressed in Xenopus ooctyes. Among the 4-amino derivatives, a representative atypical type I PAM 6p exhibits potent activation of α7 current with an EC50 of 1.3 μM and the maximum activation effect on the current over 48-fold in the presence of acetylcholine (100 μM). The structure–activity relationship (SAR) analysis reveals that the 4-amino group is crucial for the allosteric activation of α7 currents by compound 6p as the substitution of 4-methyl group results in its conversion to compound 7b (EC50 = 2.1 μM; max effect: 58-fold) characterized as a typical type I PAM. Furthermore, both 6p and 7b are able to rescue auditory gating deficits in mouse schizophrenia-like model of acoustic startle prepulse inhibition.
研究领域:
神经科学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NEUROTRAUMA
·
2023
·
IF 4.2
Hongxing Tang,Kejun He,Kun Zhao,Chen Zheng,Weichi Wu,Weilin Jin,Lixuan Yang,Baoshu Xie
Hinokitiol (β-thujaplicin, HIK) is a natural monoterpene small molecule compound showing various biological effects including antibacterial, anti- inflammatory and anti-tumor. Ferroptosis, a new form of iron-dependent cell death, has been demonstrated as one of the pathophysiological mechanisms of traumatic brain injury (TBI). However, the effect of hinokitiol on neuronal ferroptosis after traumatic brain injury has not been reported. Here, we show that hinokitiol alleviated excessive glutamate-induced intracellular reactive oxygen species (ROS), lipid peroxidation, and Fe2+ accumulation in HT-22. Moreover, hinokitiol upregulated heme oxygenase-1 (HO-1) expression, promoted nuclear factor-erythroid factor 2-related factor 2 (Nrf2) nuclear translocation, and inhibited the activation of microglia and astrocyte after TBI. Furthermore, hinokitiol remarkably reduced brain tissue lesions, mitigated neuron loss, iron deposition and attenuated neurologic impairment in the CCI-constructed TBI model in vivo. These results suggest that hinokitiol has neuroprotective effects on rescuing cells from TBI-induced neuronal ferroptosis. In summary, we reveal a novel role of hinokitiol in TBI, which has neuroprotective effects on rescuing cells from TBI-induced neuronal ferroptosis. Moreover, hinokitiol through activating the Nrf2/Keap1/HO-1 signaling pathway significantly reduces the neuronal damage, suggesting that it is a potential therapeutic candidate for TBI.
研究领域:
神经科学
·
药理学
·
细胞生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Green Synthesis and Catalysis
·
2020
·
IF 0
Bingmei Su,Lian Xu,Xinqi Xu,Lichao Wang,Aipeng Li,Juan Lin,Lidan Ye,Hongwei Yu
As an important building block for the synthesis of angiotensin-converting enzyme inhibitors, ethyl ( R )-2-hydroxyl-4-phenylbutanoate [( R )-HPBE] has attracted increasing attention. The key to industrial biosynthesis of ( R )-HPBE is a biocatalyst that efficiently reduces ethyl 2-oxo-4-phenylbutanoate (OPBE) with high R -enantioselectivity. This paper proposed a strategy for identifying key residues involved in enantioselectivity control based on per-residue free energy decomposition and sequence conservatism analysis. Using this strategy, 4 nonconservative sites with high energy contribution to binding of OPBE were chosen as engineering targets, generating variant Mu27 with 99% conversion and 98% ( R ) ee value at substrate loading of up to 500 mmol/L. MD simulations suggested the higher stability and formation probability of Mu27-OPBE proR prereaction state as key reasons for the excellent R -enantioselectivity of Mu27 towards OPBE. The success in this study provides a viable approach for rational design of alcohol dehydrogenases with high enantioselectivity towards unnatural substrates.
研究领域:
生物化学
·
酶工程
·
计算生物学
✓ 已验证
DOI
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2026
·
IF 2.4
Zhen Chang,Yaxuan Zhen,Chunying Zhu,Taotao Fu,Youguang Ma
The densities and dynamic viscosities of pure components, binary solutions and ternary solutions of 2-amino-2-methyl-1-propanol (AMP), 2-ethylaminoethanol (EAE) and water were systematically measured at (293.15–323.15) K. Based on experimental findings, calculations were made for the excess molar volume, thermal expansion coefficient, dynamic viscosity deviation, and solution viscous activation energy to assess intermolecular interactions. The negative excess molar volumes of binary and ternary aqueous solutions indicate significant hydrogen bonding interaction and a filling effect within the solutions. The negative excess molar volumes of binary and ternary aqueous solutions indicate significant hydrogen bonding interactions and a filling effect within the solutions. For the AMP–EAE system, the hydrogen bonding interaction and filling effect are weakened due to the similar volume and steric hindrance effect.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）