ACS Chemical Neuroscience
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2021
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IF 4.418
Yang Zhang,Kexin Li,Xiangyi Wang,Yanyu Ding,Zhiruo Ren,Jinglong Fang,Tao Sun,Yan Guo,Zhiwu Chen,Jiyue Wen
The effect of cystathionine-γ-lyase (CSE)-derived hydrogen sulfide (H2S) on the reactive proliferation of astrocytes and neural functional recovery over 30 d after acute cerebral ischemia and reperfusion (I/R) was determined by applying wild-type (WT) and CSE knockout (KO) mice. The changes of glial fibrillary acidic protein (GFAP) expression in hippocampal tissues was tested. Besides, we assessed the changes of mice spatial learning memory ability, neuronal damage, RhoA, Rho kinase 2 (ROCK2), and myelin basic protein (MBP) expressions in hippocampal tissues. The results revealed that cerebral I/R resulted in obvious increase of GFAP expression in hippocampal tissues. Besides, we found the neuronal damage, learning, and memory deficits of mice induced by cerebral I/R as well as revealed the upregulation of RhoA and ROCK2 expressions and reduced MBP expression in hipppcampal tissues of mice following cerebral I/R. Not surprisingly, the GFAP expression and cerebral injury as well as the upregulation of the RhoA/ROCK2 pathway were more remarkable in CSE KO mice, compared with those in WT mice over 30 d following acute cerebral I/R, which could be blocked by NaHS treatment, a donor of exogenous H2S. In addition, the ROCK inhibitor Fasudil also inhibited the reactive proliferation of astrocytes and ameliorated the recovery of neuronal function over 30 d after cerebral I/R. For the purpose of further confirmation of the role of H2S on the astrocytes proliferation following cerebral I/R, the immunofluorescence double staining: bromodeoxyuridine (BrdU) and GFAP was evaluated. There was a marked upregulation of BrdU-labeled cells coexpressed with GFAP in hippocampal tissues at 30 d after acute cerebral I/R; however, the increment of astrocytes proliferation could be ameliorated by both NaHS and Fasudil. These findings indicated that CSE-derived H2S could inhibit the reactive proliferation of astrocytes and promote the recovery of mice neural functional deficits induced by a cerebral I/R injury via inhibition of the RhoA/ROCK2 signal pathway.
研究领域:
神经科学
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药理学
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分子生物学
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细胞生物学
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高影响因子
FOOD CHEMISTRY
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2026
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IF 10.4
Sitong Zhao,Xu Yao,Yahua Zhu,Kai Wang,Haiquan Zhang
The detection of trace hydrogen sulfide (H 2 S) enables early warning of food spoilage, serving as a key technology for food safety and quality control. Here, with the research purpose of developing a simple, rapid and sensitive method for real-time monitoring of the freshness of sulfur-containing protein foods, a poly(allylamine)-stabilized perylene diimide radical anion (QFPDI-PAA, a stable π-radical) is reported which can be used colorimetric detection of H 2 S and monitoring spoilage process of sulfur-containing protein. The colorimetric mechanism is that H 2 S induced the intramolecular and intermolecular aggregation in QFPDI-PAA promotes the aggregation of perylene diimide radical fragments. Its detection limit for H 2 S in the naked-eye is 3 μM, and the spectral detection limit is as low as 0.184 μM. In real food detection, this radical achieved real-time monitoring of the production of H 2 S during the deterioration process of Short-Necked Clams, eggs, chicken and shrimp products. The visual detected times at 25 °C are as follows: raw yolk 60 h, raw mixed egg liquid 72 h, cooked yolk 60 h, cooked egg-white 36 h, cooked mixed egg pieces 24 h, raw clams 25 h, cooked Short-Necked Clams 35 h, raw chicken 12 h, raw shrimp 9 h. The visual inspection times at 0 °C are as follows: raw chicken 72 h, raw shrimp 24 h. This study establishes, for the first time, an effective strategy for the food spoilage monitoring by stable π-radicals.
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高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Meiyi Du,Dandan Shi,Kai Zhao,Zhaoliang Hu,Qian Zhou,Zhaoxia Wu,Bing Bai
Tyrosinase (TY) drives melanin accumulation and enzymatic browning in produce. This study explored the inhibition mechanism of garlic-derived H 2 S on TY and its feasibility in preventing Agaricus bisporus browning. Garlic sulfides, including diallyl trisulfide (DATS), diallyl disulfide (DADS), and diallylthiosulfinate (Allicin), all inhibited TY activity via reduced glutathione (GSH)-induced H 2 S release, with DATS exhibiting the strongest effect due to its high Sn 0 content. H 2 S bound TY at a single site, disrupted hydrogen bonds and hydrophobic interactions, reducing α-helix content, weakening van der Waals forces, inducing Cys83 persulfidation, destabilized the flexible loop, and increased the CuA-CuB distance. The “Nucleophilic attack leading to flexible loop collapse and persulfidation” mechanism enriches the biochemical theory of H 2 S as enzyme inhibitors. Additionally, garlic-derived H 2 S effectively reduced browning, malondialdehyde levels, PPO/POD activity, and volatile flavor deterioration in Agaricus bisporus homogenate, providing a novel strategy for natural TY inhibitors and fresh-produce preservation.
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高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2018
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IF 6.653
Jianliang Sun,Lianghai Li,Guangying Zhou,Xue Wang,Liang Zhang,Yueping Liu,Jierui Yang,Xianghong Lü,Feng Jiang
The conventional flue gas treatment technologies require high capital investments and chemical costs, which limit their application in industrial sectors. This study developed a sulfur-cycling technology to integrate sulfide production by biological sulfur reduction and simultaneous catalytic desulfurization and denitrification with H2S (H2S-SCDD) for flue gas treatment and sulfur recovery. In a packed bed reactor, high-rate sulfide production (1.63 ± 0.16 kg S/m3-d) from biological sulfur reduction was achieved using organics in wastewater as electron donors at pH around 5.8. 93% of sulfide in wastewater was stripped out as H2Sg, which can be a low-cost reducing agent in the H2S-SCDD process. Over 90% of both SO2 and NO were removed by the H2S-SCDD process under the test conditions, resulting in the formation of sulfur. 88% of the input S (H2Sg and SO2) were recovered as octasulfur with high purity. Besides partial recycling to produce biogenic sulfide, excessive sulfur can be obtained as a sellable product. The integrated sulfur-cycling technology is a chemical-saving and even profitable solution to the flue gas treatment in industrial sectors with wastewater available.
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Food Packaging and Shelf Life
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2022
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IF 8.749
Shuo Shi,Xiaowei Xu,Jia Feng,Yanming Ren,Xue Bai,Xiufang Xia
The pH, ammonia, and H 2 S sensitivity of sodium alginate/black soybean seed coat anthocyanin (SA/BSSCA) films and their response mechanism were analyzed based on stability, intermolecular interactions, and microstructure. The results showed that the with the BSSCA content increased from 0.02% to 0.30%, SA/0.02–0.12BSSCA films had the highest color stability. The highest sensitivity to pH, ammonia, and H 2 S was observed for the SA/0.12–0.30BSSCA films, meanwhile, the SA/0.12BSSCA film had the highest sensitivity to meat unfreshness and spoilage. The incorporation of BSSCAs reduced the water solubility, water vapor absorption, and permeability of the films. With the BSSCA addition, the intermolecular interaction between BSSCAs and SA intensified, reducing the film roughness and number of aggregated particles, resulting in compact microstructures, which improved the film sensitivity. Thus, the SA/0.12BSSCA film was sensitive to pH, ammonia, and H 2 S and, therefore, meat spoilage, while having higher stability, moderate water barrier properties, and denser microstructure.
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高影响因子
TALANTA
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2021
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IF 6.057
Wenpeng Gong,Li Zhang,Ying Yu,Bixia Lin,Yumin Wang,Manli Guo,Yujuan Cao
Hydrogen sulfide is typical metabolic marker and environmental pollutant which is worthwhile to determine. Herein, a low background and high sensitivity fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH 2 is proposed for the determination of hydrogen sulfide. Firstly, a functional monomer 3,5-diaminobenzoic acid is employed to modify on the CAU-10-NH 2 , the product CAU-10-NH-dAba has strong fluorescent performance at 412 nm under an excitation wavelength of 320 nm. Subsequently, it is further modified by the azide group to form CAU-10-NH-dAba-N 3 . This azidation inhibits the fluorescent signal. However, in the presence of hydrogen sulfide, the azide group is specifically reduced to amidogen, and results in the recovery of the fluorescence. The CAU-10-NH-DABA-N 3 was characterized by solid state NMR , XPS, fluorescence, IR, XRD, SEM and specific surface area. After the optimization of pH value, temperature and interaction time, the detection results of hydrogen sulfide demonstrate the linear range of this strategy is from 20 to 140 nM with a detection limit of 1.51 nM, which is significantly better than that of the CAU-10-NH 2 merely modified by 3,5-dinitrobenzoic acid. Meanwhile, the satisfactory assay results of hydrogen sulfide in serum sample and Pearl river water suggest a potential application prospect of this strategy in clinical diagnosis and environment monitoring.
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BIOMEDICINE & PHARMACOTHERAPY
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2019
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IF 3.743
Zhongchao Wang,Ying Yan,Yini Wang,Fei Tong
Introduction The purpose of the experiment was to survey the therapeutic function of resveratrol (RES)-loaded poly(ethylene glycol)-poly(phenylalanine) (PEG-PPhe) on intestinal ischemia/reperfusion injury (II/RI) via the interaction between CSE/H 2 S and iNOS/NO compared to free RES in diabetic rats. Methods Diabetic rats were pretreated with 20 mg/kg of RES or the RES/PEG-PPhe complex and then subjected to 1 h of ischemia and 3 h of reperfusion. Blood and intestines were collected, intestinal pathological injury was estimated, and the contents of body weight, weights of different tissues, blood glucose, serum insulin, HOMA index, serum nitric oxide (NO) and serum sulfureted hydrogen (H 2 S) were observed. The dry/wet intestine ratios, the activity of superoxide dismutase (SOD); the contents of methane dicarboxylic aldehyde (MDA), glutathione (GSH), H 2 S, and NO; and the concentrations of inducible nitric oxide synthase (iNOS) and cystathionine-γ-lyase (CSE) were observed in the intestinal tissues. Results A significant reduction of weights of different tissues, blood glucose, pathological damage, dry/wet ratios, MDA, NO, iNOS expression and a significant increasement of body weight, serum insulin, HOMA index, SOD, GSH, H 2 S, CSE expression were observed in both treatment groups. However, a greater reduction of weights of different tissues, blood glucose (7.49–13.49 mmol/L for 72 h vs. the control) and pathological damage, iNOS expression, dry/wet ratios (6.14 ± 0.29 vs. 8.51 ± 0.42), MDA (5.01 ± 0.71 nmol vs. 9.98 ± 0.67 nmol), NO (0.52 ± 0.09 μmol vs. 0.99 ± 0.08 μmol in intestinal tissue; 19.29 ± 0.89 μmol vs. 45.23 ± 1.17 μmol in serum) was observed in the RES/PEG-PPhe group relative to the I/R ( P < 0.01 for all); a greater increasement of body weight, serum insulin, HOMA index, SOD (39.79±1.78 U vs. 11.84 ± 1.02 U), GSH (31.25 ± 1.19 mg vs. 10.13 ± 0.64 mg), H 2 S (39.52 ± 1.32 nmol vs. 13.02 ± 1.03 nmol in intestinal tissue; 9.78 ± 0.79 μmol vs. 3.11 ± 0.85 μmol in serum), CSE expression was observed in the RES/PEG-PPhe group relative to the I/R ( P < 0.01 for all). In addition, aminoguanidine (AMI, iNOS inhibitor) reduced I/R injury, and dl -propargylglycine (PAG, CSE inhibitor) increased I/R injury. Conclusions The interaction between CSE/H2S and the iNOS/NO-mediated resveratrol/poly(ethylene glycol)-poly(phenylalanine) complex alleviates intestinal ischemia/reperfusion injuries in diabetic rats.
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高影响因子
BIOMATERIALS
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2025
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IF 12.9
Liyang Gong,Le Chang,Siyu Chen,Xuan Wei,Huiping Du,Jiamin Cheng,Xiaoxuan Chen,Zhang Yuan,Pan Zhao,Meijuan Geng,Hui Yang,Kaiyong Cai,Liangliang Dai
Hydrogen sulfide (H 2 S) gas therapy is beneficial for accelerating wound healing and alleviating the inflammatory process, but is seriously hindered by insufficient delivery and unsustainable release in vivo . This study presents a multifunctional injectable hydrogel, OC@ε-PL-SATO, composed of oxidized hyaluronic acid and N-acetylcysteine (NAC) as an initiator, carboxymethyl chitosan and S-aroylthiooxime modified ε-Poly-( l -lysine) (ε-PL-SATO). ε-PL-SATO is a NAC-responsive H 2 S donor. OC@ε-PL-SATO hydrogel is designed for the desired wound healing process, with rapid gelation (<30 s) and a sustained H 2 S release. After mixing and gelling, H 2 S could be long-term released from the hydrogel and effectively drives macrophages toward M2 polarization, thereby ameliorating the inflammatory response. Revealed by transcriptome analysis, the underlying mechanism is that OC@ε-PL-SATO hydrogel releasing H 2 S inhibits LPS-mediated inflammatory responses in RAW264.7 cells by interfering with phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling and NF-κB activation. Furthermore, the OC@ε-PL-SATO hydrogel effectively eliminates the bacterial burden and alleviates the accompanying inflammation in a rat model of cutaneous wound infection. Importantly, the sustained generation of H 2 S gas significantly promotes angiogenesis and collagen deposition, ultimately accelerating the wound repair. In conclusion, this study provides a multifunctional injectable hydrogel with rapid gelatinization and continuous H 2 S release for accelerating the infected wound healing.
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高影响因子
Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Yanmei Cui,Yerong Huang,Jie Mi,Jiancheng Wang,Mengmeng Wu
It is unclear about H 2 O-induced effects on H 2 S-absoprion/COS-release behavior during high-temperature coal gas cleaning with Zn-based sorbents. This work aims at clarifying the aforementioned effect. The results reveal that the presence of 5–10 vol% H 2 O reduces the sulfur capacity of sorbents by 41–55%, while also decreasing the amount of released COS before H 2 S-breakthrough ( A-COS BT ) by 97%–99%. For wet coal gas desulfurization, the CO-H 2 S reaction accounts for nearly all of the COS released, while the contribution from the CO 2 -H 2 S reaction is negligible. Both Ni- and Ce-doping decrease A-COS BT of sorbents by 85%–89% and 92%–94%, respectively. Moreover, Ni-doping enhances the H 2 S-uptake capacity of sorbents by 32%–103%, which is mainly ascribed to the weakening of Zn-O bond. The performance of the Ni-Ce co-doped desulfurizer lies between those of the sorbents doped solely with Ni and Ce, respectively. Furthermore, co-doped sorbents could maintain 82%–92% of initial sulfur capacity and strong ability of inhibiting COS-formation during multiple sulfidation-regeneration cycles. It provides new insights for H 2 O-induced effects on H 2 S-absorption/COS-release behavior during hot coal gas cleaning.
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MATERIALS RESEARCH BULLETIN
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2023
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IF 5.4
Zhen Zhao,Lei Zhang,Hongming Zhang,Wuhong Xin,Xiaowen Wang,Chunmiao Liu,Yang Guo,Yinghua Zhuang,Wanli Jiao
Investigating high-performance and low-energy consumption H 2 S sensors has become a hot research topic. This study adopted a one-step co-precipitation method to synthesize La-doped ZIF-8 deprotonating zinc acetate, lanthanum nitrate, and 2-Methylimidazole in an aqueous. La 2 O 3 /ZnO were synthesized with different La 3+ doping concentrations (0, 0.5, 1, and 1.5 at%). We found that La 3+ doping at a concentration of 1 at% showed the highest response value of 140 for 5 ppm H 2 S, at a low operating temperature of 100 °C, which is 2.4 times higher than that of pure ZnO. Additionally, La 3+ doping led to a lower detection limit of 1.9 ppb. The enhanced performance can be attributed to the widening of the difference in electron concentration, the increase in vacant oxygen content, and the spillover effect of H 2 S sensing. These factors were analyzed in detail by means of porosity, P-N heterojunctions, and the increased in adsorbed oxygen content caused by La 3+ doping.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Xiang Li,Xueqian Wang,Langlang Wang,Li Yuan,Yixing Ma,Yibing Xie,Yiran Xiong,Ping Ning
The low S selectivity of catalysts for H 2 S removal significantly hinders its application in blast furnace gas desulfurization. In this study, Cu-based coconut shell biochar was modified with different types and contents of alkali species. The results showed that the S selectivity close to 100% was prolonged from 0 to 750 min. At the same time, the sulfur capacity was increased from 242.1 mg/g (before the modification) to 373.4 mg/g by using K 2 CO 3 -induced Cu-based biochar at 120 °C. In CO, H 2 O, and O 2 atmosphere, H 2 S was dissociated and oxidized to elemental S. Additionally, K 2 CO 3 exhibited an elemental S anchoring effect, making the S more stable at medium–high temperatures and weakening the reactivity of S with CO and ·O 2 – . Thus, less COS and SO 2 were generated. Moreover, the basic sites enhanced by K 2 CO 3 promoted the hydrolysis of COS to generate H 2 S, which entered the desulfurization cycle. K 2 CO 3 also showed a synergistic effect with the active components, generating more ·O 2 – to promote the oxygenation of H 2 S. The regeneration efficiency of the catalyst could be stabilized above 90%. These findings could provide guidance and reference for designing and applying desulfurization catalysts with high S selectivity and activity.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Qiang Chen,Chunjing Guo,Xiudi Zhou,Yanguo Su,Huimin Guo,Min Cao,Jing Li,Yue Zhang,Weiyi Zhao,Xin Gao,Shuqi Mi,Daquan Chen
Currently, very limited therapeutic approaches are available for the drug treatment of atherosclerosis(AS). H 2 S-donor is becoming a common trend in much life-threatening research. Several studies have documented that H 2 S-lyase is predominantly present in endothelial cells. N-Acetylneuraminic acid (SA), natural carbohydrate, binds specifically to the E -selectin receptor of endothelial cells. Meanwhile, recent studies related to Chondroitin sulfate have excellent target binding ability with CD44 receptor. We conjecture that the N-Acetylneuraminic acid and Chondroitin sulfate modified nanomicelles not only enhances the accumulation of the drug but also cleaves the H 2 S donor in the lesion, thus one stone two birds. Given these findings, we synthesized two kinds of nanoparticles, Carrier I (SCCF) and Carrier II (SCTM), for atherosclerosis to validate our guesses. Initially, S-allyl-L-cysteine and 4-methoxyphenylthiourea were used as H 2 S donors for SCCF and SCTM, respectively. After the introduction of ROS-sensitive groups. Then, micelles with N-Acetylneuraminic acid and Chondroitin sulfate were prepared to load rapamycin(RAP). Further, in atherosclerosis Oil Red O staining (ORO) results confirmed remarkable treatment effect with [email protected] and [email protected] Thus, we conclude that the effect of dual-targeting nanomicelles with ROS-sensitive H 2 S donor based on N-Acetylneuraminic acid and Chondroitin sulfate will have a better role in atherosclerosis.
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高影响因子
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