INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 2
·1081
·
IF 4.9
Yi Liu,Yang Ge,Yanfan Wu,Yongtong Feng,Han Liu,Wei Cao,Jinsong Xie,Jingzhong Zhang
Three-dimensional (3D) hepatocyte models have become a research hotspot for evaluating drug metabolism and hepatotoxicity. Compared to two-dimensional (2D) cultures, 3D cultures are better at mimicking the morphology and microenvironment of hepatocytes in vivo. However, commonly used 3D culture techniques are not suitable for high-throughput drug screening (HTS) due to their high cost, complex handling, and inability to simulate cell–extracellular matrix (ECM) interactions. This article describes a method for rapid and reproducible 3D cell cultures with ECM–cell interactions based on 3D culture instrumentation to provide more efficient HTS. We developed a microsphere preparation based on a high-voltage electrostatic (HVE) field and used sodium alginate- and collagen-based hydrogels as scaffolds for 3D cultures of HepG2 cells. The microsphere-generating device enables the rapid and reproducible preparation of bioactive hydrogel microspheres. This 3D culture system exhibited better cell viability, heterogeneity, and drug-metabolizing activity than 2D and other 3D culture models, and the long-term culture characteristics of this system make it suitable for predicting long-term liver toxicity. This system improves the overall applicability of HepG2 spheroids in safety assessment studies, and this simple and controllable high-throughput-compatible method shows potential for use in drug toxicity screening assays and mechanistic studies.Keywords:hepatocyte;3D cultures;hydrogel;microspheres;high-voltage electrostatic field;RNA-seq
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高影响因子
CARBON
·
2024
·
IF 10.5
Sikui Chen,Ce Cui,Jiatong Yan,Chuanxi Lin,Shan Jiang,Hong Tang,Ronghui Guo
The increasing proliferation of modern communications, radar systems, and military equipment operating in the X and Ku bands (8–18 GHz) has exacerbated the issue of electromagnetic wave (EMW) pollution, highlighting the urgent need for lightweight, broadband EMW-absorbing materials. In this paper, the lightweight carbon fiber aerogel@hollow carbon/Co 3 O 4 microsphere (CFA@H–C/Co 3 O 4 ) were composed by ZIF-67 derived hollow carbon/Co 3 O 4 microsphere and bamboo cellulose fiber derived carbon fiber aerogels and constructed by in-situ chemical deposition, dopamine treatment and pyrolysis. Carbon fiber aerogels form a lightweight three-dimensional (3D) interconnected conductive network, which expands the multiple reflection and absorption paths of EMW and increases the dielectric loss. The hollow carbon/Co 3 O 4 microsphere inhibits the collapse of the structure by forming a polydopamine shell through the self-polymerization effect of dopamine on the surface of ZIF-67 during pyrolysis, showing dipole polarization loss, interface polarization loss and magnetic loss, which plays an important role in improving EMW polarization loss and optimizing impedance matching. The minimum reflection loss (RL min ) of CFA@H–C/Co 3 O 4 reaches −43.5 dB at frequency of 12.88 GHz with thickness of 3.0 mm and the effective absorption bandwidth (EAB) of CFA@H–C/Co 3 O 4 reaches 7.84 GHz (10.08–17.92 GHz) with thickness of 3.0 mm, covering most of X and Ku bands at a low filling ratio of 15 wt%. The radar cross-section (RCS) value of CFA@H–C/Co 3 O 4 is 22.68 dB m 2 lower than the perfect electrical conductor (PEC). This study provides valuable insights into materials that can improve the absorption bandwidth of electromagnetic waves in the X and KU bands.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Yafang Zhao,Jun Zheng,Yanmei Zhao,Kai Zhang,Wenwu Fu,Gang Wang,Haodong Wang,Yaowei Hao,Zhiguang Lin,Xiaocao Cao,Jiayi Liu,Ming Zhang,Zhongrong Shen
Hard carbon as a negative electrode material for sodium-ion batteries (SIBs) has great commercial potential and has been widely studied. The sodium-ion intercalation in graphite domains and the filling of closed pores in the low voltage platform region still remain a subject of controversy. We have successfully constructed hard carbon materials with a pseudo-graphitic structure by using polymerizable p -phenylenediamine and dichloromethane as carbon sources. This was achieved by a halogenated amination reaction and oxidative polymerization. It was found that the capacity of hard carbon materials mainly originates from intercalation into graphite domains. The study found that the prepared hard carbon could store 339.33 mAh g −1 of sodium in a reversible way at a current density of 25 mA g −1 , and it had an initial coulomb efficiency of 80.23%. It even maintained a reversible sodium storage capacity of 125.53 mAh g −1 at a high current density of 12.8 A g −1 . Based on the analysis of hard carbon structure and electrochemical performance , it was shown that the materials conform with an “adsorption-intercalation” mechanism for sodium storage.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Ziheng Wei,Xiaoli Yang,Lingrui Xu,Lamu Benma,Danhong Wu,Hulie Zeng
The detection of low-abundance microribonucleic acid (miRNA) frequently adopted nucleic acid sequence-based amplification detection, which was found to have poor selectivity for the nonspecific amplification of template-dependent ligation in enzyme-mediated cascade reactions. Here, a highly selective detection of miRNAs was developed that combined microsphere-enhanced fluorescence (MSEF) and solid-phase base-paired hybridization. The target miRNA could be accurately and quantitatively identified through the solid-phase hybridization assay on the surface of an optical microsphere, while the detected fluorescence signal could be physically amplified by MSEF. Hereinto, the optical microsphere acted as the fluorescence amplifier and whose surface supplied the space to carry out base-paired hybridization to recognize the target miRNA via the immobilized capture DNA sequence. The detected fluorescence signal of the single-base mismatched miRNA-21 sequence was just around 12% of that of the target miRNA-21 sequence in the measurement of model miRNA-21, while the limit of detection of miRNA-21 could be 1.0 fM. The developed detection of miRNA on an optical microsphere was demonstrated to be an excellent physically amplified method to selectively and sensitively detect the target miRNA and magnificently avoid the nonspecific amplification and false-positive results, which is expected to have wide applications in pathematology, pharmacology, clinic diagnosis, and on-site screening fields as well.
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JOURNAL OF LIGHTWAVE TECHNOLOGY
·
2024
·Vol. 42
·期 16
·5768-5774
·
IF 4.1
Xuegang Li,Huiying Sun,Yanan Zhang,Yong Zhao,Xue Zhou
In this paper, a groundbreaking probe-type fiber optic biosensor, harnessing the surface plasmon resonance (SPR) effect enhanced by barium titanate glass microspheres, is unveiled for virus RNA detection. The multi-physics simulation was used to verify the sensitivity improvement achieved by introducing barium titanate microspheres on the surface of the optical fiber microspheres probe. Experiments confirmed this, pushing the sensor's limit to an impressive 6802 nm/RIU. Biomarker capture on the microsphere surface subtly alters the local refractive index, triggering a detectable SPR spectral shift. To showcase its prowess, the sensor tackled the reverse-transcribed complementary DNA (cDNA) of COVID-19 RNA. Chemical modification of the gold film and DNA hybridization technology ensured specific cDNA detection. The limit of detection for this target reached a remarkable 1.58 nmol/L. This sensor not only excels in virus RNA detection but also holds immense potential for diagnosing major diseases by pinpointing the concentrations of key biomarkers.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Xin Jiang,Rui Wang,Yimin Tang,Weijie Di,Wenxue Wang,Bing Zhao,Libin Yang
Combined contribution of electromagnetic and chemical effects and their synchronous tuning are an effective strategy for constructing semiconductor surface-enhanced Raman scattering (SERS) substrates with ultra-high sensitivity. In this work, a sulfur vacancy-rich MoS 2 flower-like microsphere is successfully prepared for the first time through the combination of morphology regulation and defect engineering strategies, achieving a synchronous contribution of electromagnetic and chemical effects to SERS enhancement. SERS enhancement factor is as high as 2.54 × 10 8 , which represents the highest sensitivity among the currently reported semiconductor SERS-active substrates. The theoretical calculations and experiments elucidate the observed enhancement activity and the synchronous enhancement mechanism of electromagnetic and chemical effects. The unique flower-like structures of MoS 2 can induce Mie resonance by multiple reflection and scattering of incident light in the cavity structure, which realizes a strong electromagnetic enhancement effect. Meanwhile, a high-efficient carrier separation in substrate and a multiple-channel charge transfer mode between substrate and analyte can be achieved by means of abundant surface sulfur-vacancy defects, which provide a strong chemical enhancement effect for target analyte. This work opens up a new idea and perspective for constructing supersensitive semiconductor SERS sensors, ultimately advancing practical application of semiconductor SERS technology.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Jiaqing Zhang,Yong Long,Xuetong Duan,Jiyan Li,An Li
The intermittent nature of solar energy supply causes photothermal materials in interfacial evaporation systems to be unable to achieve continuous and stable photothermal conversion and water supply functions. To this end, this paper constructs a Janus composite membrane (MSHS@TDA@PAN) with both photothermal conversion and thermal energy storage capabilities through electrospinning. In MSHS@TDA@PAN, MgO/Pt multi-shell hollow microspheres impregnated with tetradecylamine (TDA) form a hydrophobic photothermal layer, while hydrophilic polyacrylonitrile (PAN) nanofibers act as a water-transporting layer, together driving efficient photothermal interfacial evaporation. It achieves an intrinsic light absorption of 80.7 %, a photothermal conversion efficiency of 79.83 %, and a phase change enthalpy of 90.7 J·g −1 . Experimental results show that the average evaporation rate of the photothermal membrane over one phase change cycle (One hour each for solar irradiation and dark field) is increased from 2.402 kg·m −2 of the membrane without phase change materials to 2.67 kg·m −2 . It can increase the production of fresh water by 3.24 kg·m −2 ·d −1 . Especially under no-light conditions, the evaporation rate of MSHS@TDA@PAN is 2.1 times that of the control group, which significantly alleviates the performance attenuation caused by intermittent illumination. In conclusion, MSHS@TDA@PAN effectively solves the intermittency problem of solar energy utilization through the synergistic mechanism of photothermal conversion, thermal energy storage and continuous heat release. It has application prospects in the fields of continuous seawater desalination and photothermal utilization.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Xuechen Tang,Bobo Zhou,Chao Chen,Bauyrzhan Sarsenbekuly,Hongbin Yang,Wanli Kang
Due to the superior performance caused by surface modification and combined effects, polymer/nano-SiO 2 composites have been studied systematically for a long term and attracted increasing academic and industrial interests, especially in the petroleum engineering field. However, few works have been done on the mechanism that how the functional modification degree ( MD ) of nano-SiO 2 influences on the properties of the composites. In this work, a series of 3-(methacrylyloxy)propyltrimethoxysilane (MPS) modified nano-SiO 2 particles with different MD s were prepared and found to differ largely from each other in macroscopic properties . Then the effects of SiO 2 MD on the physicochemical property and the enhanced oil recovery (EOR) capacity of the corresponding polymer/nano-SiO 2 composite microspheres (PNSCMs) for the conformance control in oilfields were investigated in detail. The results clarified that by regulating the MD of nano-SiO 2 encapsulated in PNSCMs, the swelling property, viscoelasticity , mechanical strength and dispersion stability were significantly improved. Compared with traditional chemical copolymer microspheres CPMs, PNSCMs exhibited better conformance control and EOR ability, which was further improved as SiO 2 MD increased. The maximum oil recoveries of cores treated by PNSCMs were 76.14% and 52.45% in the experiment, and the EOR increased by 5.99% and 4.99% than those treated by CPMs, respectively, under homogeneous and heterogeneous conditions.
研究领域:
石油工程
·
材料科学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
·
IF 2.857
Gaoshuang Hu,Wei Sheng,Yan Zhang,Junping Wang,Xuening Wu,Shuo Wang
A novel fluorescence immunoassay for detecting sulfaquinoxaline (SQX) in animal-derived foods was developed using NaYF4:Yb/Tm upconversion nanoparticles (UCNPs) conjugated with antibodies as fluorescence signal probes, and monodisperse magnetic polystyrene microspheres (MMPMs) modified with coating antigen as immune-sensing capture probes for trapping and separating the signal probes. Based on a competitive immunoassay format, the detection limit of the proposed method for detecting SQX was 0.1 μg L–1 in buffer and 0.5 μg kg–1 in food samples. The recoveries of SQX in spiked samples ranged from 69.80 to 133.00%, with coefficients of variation of 0.24–25.06%. The extraction procedure was fast, simple, and environmentally friendly, requiring no organic solvents. In particular, milk samples can be analyzed directly after simple dilution. This method has appealing properties, such as sensitive fluorescence response, a simple and fast extraction procedure, and environmental friendliness, and could be applied to detecting SQX in animal-derived foods.
研究领域:
分析化学
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食品科学
·
纳米技术
·
生物技术
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高影响因子
TALANTA
·
2017
·
IF 4.162
Shiyu Li,Kui He,Rong Liao,Chunyan Chen,Xiaoming Chen,Changqun Cai
An interference-free and label-free sensing platform was developed for the highly sensitive detection of microRNA-21 (miRNA-21) in vitro by magnetic silicon microsphere (MNP)-reduced graphene oxide (rGO)-based sandwich probe. In this method, DNA capture probes (P 1 ) were connected with MNPs at the 5′ end and hybridized with completely complementary target miRNA. Subsequently, rGO was retained and induced the fluorescence quenching in the supernatant. Through the magnetic separation, the supernatant environment was simplified and the interference to analytical signal was eliminated. When DNA capture probe-modified magnetic silicon microspheres (MNP-P 1 ) were adsorbed through rGO in the absence of a target and formed a sandwich structure, the formed nanostructure was easily removed from the solution by a magnetic field and the fluorescence intensity was maximally recovered. This proposed strategy, which both overcame the expensive and cumbersome fluorescent labeling , and eliminated interference to analytical signal for guaranteeing high signal-to-background ratio, exhibited high sensitivity with a detection limit as low as 0.098 nM and special selectivity toward miRNA-21. The method was potentially applicable for not only detection of miRNA-21 but also various biomarker analyses just by changing capture probes.
研究领域:
生物传感器
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纳米技术
·
分子诊断
·
分析化学
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高影响因子
RSC Advances
·
2013
·Vol. 3
·期 19
·6871-6878
·
IF 2.562
Delin Cheng,Xiaodong Cao,Huichang Gao,Yingjun Wang
Biodegradable polymer microspheres have been increasingly attracting attention in bone repair. In this work, superficially porous poly(lactic-co-glycolic acid)/calcium carbonate (PLGA/CC) composite microsphere is prepared by spontaneous pore-forming emulsion method. In addition to being the component that neutralizes the acidic products from PLGA degradation, calcium carbonate (CC) also constructs macropores on the surface of microsphere and thus exempts the use of extraneous porogens, which simplifies the preparation process and avoids problems related to extraneous porogen. It is found that the size of CC plays the most essential role in forming the superficial macropores because it determines the water absorption capacity of CC. Release of dexamethasone from the superficially porous composite microspheres shows a mild burst and the subsequent tri-phase profile. Meanwhile, the cell culture test demonstrates that MG-63 cells grow well on the superficially porous composite microsphere and proliferate better than those on pure PLGA microsphere. Therefore, this versatile composite microsphere has a promising application for bone repair.
研究领域:
生物材料学
·
医学工程
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Yang Hu,Shanshan Ma,Zhuohong Yang,Wuyi Zhou,Zhengshan Du,Jian Huang,Huan Yi,Chaoyang Wang
In this study, we develop a facile one-pot approach to the fabrication of poly(L-lactic acid) (PLLA) microsphere-incorporated calcium alginate (ALG-Ca)/hydroxyapatite (HAp) porous scaffolds based on HAp nanoparticle-stabilized oil-in-water Pickering emulsion templates, which contain alginate in the aqueous phase and PLLA in the oil phase. The emulsion aqueous phase is solidified by in situ gelation of alginate with Ca 2+ released from HAp by decreasing pH with slow hydrolysis of d -gluconic acid δ-lactone (GDL) to produce emulsion droplet-incorporated gels, followed by freeze-drying to form porous scaffolds containing microspheres. The pore structure of porous scaffolds can be adjusted by varying the HAp or GDL concentration. The compressive tests show that the increase of HAp or GDL concentration is beneficial to improve the compressive property of porous scaffolds, while the excessive HAp can lead to the decrease in compressive property. Moreover, the swelling behavior studies display that the swelling ratios of porous scaffolds reduce with increasing HAp or GDL concentration. Furthermore, hydrophobic drug ibuprofen (IBU) and hydrophilic drug bovine serum albumin (BSA) are loaded into the microspheres and scaffold matrix, respectively. In vitro drug release results indicate that BSA has a rapid release while IBU has a sustained release in the dual drug-loaded scaffolds. In vitro cell culture experiments verify that mouse bone mesenchymal stem cells can proliferate on the porous scaffolds well, indicating the good biocompatibility of porous scaffolds. All these results demonstrate that the PLLA microsphere-incorporated ALG-Ca/HAp porous scaffolds have a promising potential for tissue engineering and drug delivery applications.
研究领域:
生物材料学
·
组织工程学
·
药物递送系统
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