ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Yongxing Hu,Pengcheng Zhang,Ashraf Ali,Haokun Tang,Lijun He,Renyong Zhao,Wenfen Zhang,Shusheng Zhang,Wenjie Zhao
Background Hydrophilic interaction liquid chromatography (HILIC) offers an alternative approach for efficient separation of polar molecules on polar stationary phases. The development of hydrophilic stationary phases with novel zwitterionic structures represents a promising approach to improving the efficiency of separations and accommodating the analysis of polar compounds in complex samples. Results This work details the preparation and thorough evaluation of the novel zwitterionic stationary phase, Sil-DBO-PS, composed of a 1,4-diazabicyclo[2.2.2]octane cation and a sulfonate anion. The chromatographic behavior of Sil-DBO-PS was investigated under varying eluent conditions, including water phase ratio, salt concentration, and pH, while interaction mechanisms of Sil-DBO-PS were explored through Van't Hoff plots, Tanaka HILIC tests, and modified linear solvation energy relationships. These studies revealed that the zwitterionic group introduced a range of interactions, including hydrophilic partitioning, hydrogen bonding, and electrostatic interactions. The Sil-DBO-PS column outperformed two commercial columns in terms of separation efficiency for polar analytes, such as nucleosides, vitamins, and benzoic acids, highlighting its enhanced capability for polar compound separation. Furthermore, Sil-DBO-PS stationary phase demonstrated satisfactory reproducibility in the separation of hydrophilic compounds. Significance and novelty The development of the Sil-DBO-PS zwitterionic stationary phase significantly enhances the separation efficiency and selectivity for polar compounds in HILIC, offering superior performance over commercial columns for challenging analytes. This novel stationary phase, with its diverse interactions, provides a promising tool for the analysis of hydrophilic and complex samples.
研究领域:
分析化学
·
色谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Autophagy
·
2026
·
IF 18.6
Haojie Zhang,Yu Kang,Tianlun Zhao,Daoqiang Huang,Xuantao Hu,Jiawei Di,Yilong Zhang,Yubao Lu,Mudan Huang,Hong Li,Senyu Yao,Bin Liu,Limin Rong
Dysfunction of the neuronal macroautophagy/autophagy-lysosome system is a critical contributor to neuronal death following spinal cord injury (SCI), but the underlying mechanisms remain elusive. Our study demonstrated that SCI induced impaired autophagic flux and lysosomal membrane permeabilization (LMP) in neurons. By combining in vivo bulk RNA sequencing with validation experiments, we observed the transient upregulation of the membrane repair factor PI4K2A, which was specifically enriched in lysosomes, after SCI. Crucially, ER-MS and IP-MS analyses revealed an interaction between PI4K2A and the endoplasmic reticulum lipid transfer protein OSBPL6/ORP6. This interaction led to the transport of phosphatidylserine (PS) to damaged lysosomal membranes, promoting LMP repair and subsequently reducing lipid droplet accumulation, which suppressed neuronal death. Furthermore, overexpression of neuronal PI4K2A in vivo, through an OSBPL6- and PS-dependent mechanism, reduced LMP-mediated lipid droplet accumulation and increased neuronal survival, thereby improving functional recovery after SCI. Collectively, our findings establish the PI4K2A-OSBPL6/ORP6-PS axis as a novel and essential mechanism for lysosomal membrane repair in neurons. This pathway is crucial for maintaining neuronal lipid homeostasis and represents a promising therapeutic target for reducing neuronal loss and improving functional recovery after central nervous system trauma.Abbreviations: AIF1/IBA1: allograft inflammatory factor 1; Baf A1: bafilomycin A1; BMS: Basso Mouse Scale; CNS: central nervous system; co-IP: co-immunoprecipitation; DEGs: differentially expressed genes; DS5: DS55980254; ESCRT: endosomal sorting complex required for transport; GFP: green fluorescent protein; HSPA5/GRP78: heat shock protein family A (HSP70) member 5; HT22: hippocampal neuronal cell line; KEGG: Kyoto Encyclopedia of Genes and Genomes; LD: lipid droplet; LC-MS: liquid chromatography-mass spectrometry; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; LGALS3/GAL3: lectin, galactoside binding, soluble 3; LLOMe: L-leucyl-L-leucine methyl ester; LMP: lysosomal membrane permeabilization; LPC: lysophosphatidylcholine; LPE: lysophosphatidylethanolamine; MFGE8/lactadherin: milk fat globule EGF and factor V/VIII domain containing; MS: mass spectrometry; NAGLU: alpha-N-acetylglucosaminidase (Sanfilippo disease IIIB); NEFH/NF200: neurofilament, heavy polypeptide; OSBPL6/ORP6: oxysterol binding protein-like 6; OSBPL8/ORP8: oxysterol binding protein-like 8; PC: phosphatidylcholine; PLA2G4A/cPLA2: phospholipase A2, group IVA (cytosolic, calcium dependent); PITT: phosphoinositide-initiated membrane tethering and lipid transport; PI4K2A: phosphatidylinositol 4-kinase type 2 alpha; PLS-DA: partial least squares discriminant analysis; PS: phosphatidylserine; PtdIns: phosphatidylinositol; PTDSS1: phosphatidylserine synthase 1; PUFAs: polyunsaturated fatty acids; RBFOX3/NeuN: RNA binding protein, fox-1 homolog (C. elegans) 3; ROS: reactive oxygen species; SCI: spinal cord injury; SQSTM1/p62: sequestosome 1; TEM: transmission electron microscopy; TGs: triglycerides
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2020
·
IF 5.647
Yang Wang,Pengcheng Gao,Yingying Wei,Yajin Jin,Si Sun,Zhifei Wang,Yong Jiang
A facile and cost-effective preparation of silver nanoparticles decorated magnetic composite for the effective catalytic degradation of 4-nitrophenol (4-NP) and Methylene blue (MB) and Rhodamine B (RhB) was investigated. Fe 3 O 4 @Polystyrene@Ag (Fe 3 O 4 @PS@Ag) catalyst was prepared via a two-step procedure. Firstly, carboxyl groups modified magnetic microspheres (Fe 3 O 4 @PS-COOH) has been successfully synthesized by microemulsion polymerization. Then Ag ions were adsorbed and in-situ reduced on the surface of Fe 3 O 4 @PS microspheres . To estimate the catalytic activity of Fe 3 O 4 @PS@Ag catalyst, the reduction experiments of MB, RhB and 4-NP were performed in the presence of NaBH 4 . The results indicated that Fe 3 O 4 @PS@Ag catalyst has a good catalytic performance and these dyes can be reduced in a very short time, which the apparent rate coefficients are 0.0089 s −1 , 0.0187 s −1 and 0.0086 s −1 for MB, RhB and 4-NP respectively. In addition, it could be easily collected from aqueous solution by a magnet so that the catalyst could be recovered and reused after the catalytic process. The catalytic activity was still high after seven cycles. This catalytic reaction is in agreement with the pseudo-first-order kinetic equation . Furthermore, the as-prepared Fe 3 O 4 @PS@Ag catalyst outperforms other catalysts in the degradation of these organic dyes.
研究领域:
化学
·
环境科学
·
纳米技术
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2020
·
IF 5.774
Zhongjuan Wang,Yunhe Li,Guohua Shen,Yuanqing Li,Xinyi Zhang,Jianfeng Gou,Xiuwen Cheng
In recent decades, persulfate activation technology has been used to degrade persistent organic pollutant. Moreover, both transition metal-based catalyst and metal-free catalyst have become more efficient activator. In this study, Cu-Fe LDH was synthesized and calcined at different temperature to form into metal oxides (CLDH). Then, the CMK/LDH and CMK/CLDH composites were fabricated by co-precipitation and sonic treatment, respectively. Furthermore, the prepared samples were used to activate PS for sulfamethoxazole degradation. Based on the several characterizations and degradation experiments, a comparative study of different catalysts was conducted and the results reached the following factors: i CMK/LDH owned the best capacity of PS activation on sulfamethoxazole decomposition, 84.9% SMX of 25 mg/L was degraded with less dosage of persulfate (0.5 g/L) and catalyst (0.15 g/L) being added. ii CMK/CLDH owned the better adaptability of initial pH value compared with CMK/LDH. Meanwhile, based on scavenger quenching experiment and chronoamperometric, it was speculated that non-radical pathway played more role in CMK/LDH composite/PS/SMX system compare with radical pathway. It was interesting to find that SO 4 • − were mainly generated by LDH while HO• were mainly produced by CMK part. However, the non-radical pathway for SMX decomposition was only ascribed to the electron bridge effect that the CMK owned. This study might provide a theoretical support for further studies on comparison about LDH and the metal oxides originating from calcination of LDH for PS activation.
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Xinqiang Chang,Tao Lin,Wei Chen,Hang Xu,Hui Tao,Yuehong Wu,Qingwen Zhang,Shunzhong Yao
The main purpose of this study was to control membrane fouling by degrading natural organic matter, mainly based on free radicals, with a dead-end ultrafiltration system integrated with pretreatment . Four advanced oxidation processes , namely, ultraviolet (UV)/Fe(II), UV/persulfate (PS), Fe(II)/PS and UV/Fe(II)/PS, were used to pretreat raw water prior to ultrafiltration. The priority of the pretreatment effect followed the order of UV/Fe(II)/PS > Fe(II)/PS > UV/PS > UV/Fe(II). In the UV/Fe(II)/PS pretreatment (Fe(II) = 100 μM and PS = 400 μM), the removal rates of UV 254 with a UV irradiation time of 60 min reached 93.07%. Degradation experiments of free-radical probes (carbamazepine) and free-radical scavenger addition (sodium hyposulfite or tert-butanol) showed that the sulfate radical (SO 4 − ) was dominant in degrading organic compounds. The specific flow rate ( J / J 0 ) increased by 139.13% and the irreversible fouling resistance was reduced by 69.94%. The total interfacial energy of the colloid-membrane interaction decreased by 84.42% and the separation distance was shortened to ~2 nm. The release of Fe(III) from water under UV radiation and its possible conversion to Fe(II) were observed on the surface of the fouled membranes. After UV/Fe(II)/PS pretreatment, bulky and rough pollutant particles were transformed into a slew of sheet-contaminated layer with the appearance of numerous permeable holes, and the average surface roughness was reduced to 38.1 nm according to atomic-force-microscopy characterization.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Min-Yong Lee,Wen-Long Wang,Zi-Bin Xu,Bei Ye,Qian-Yuan Wu,Hong-Ying Hu
Dodecyltrimethylammonium chloride (DTAC) is a quaternary ammonium compound (QAC) that is a widespread contaminant in environmental media and therefore of increasing concern. The synergistic effect with UV irradiation and persulfate (UV/PS) was used to degrade DTAC . The removal of DTAC was 91% with the PS dosage of 75.6 μM (UV/PS) and UV fluence of 870 mJ·cm −2 . The second-order rate constants of DTAC with HO and SO 4 − were determined to be k HO , DTAC (4.2 ± 0.18) × 10 9 M −1 s −1 and k SO 4 ∙− , DTAC (2.5 ± 0.27) × 10 9 M −1 s −1 , respectively. The contributions of HO and SO 4 − to DTAC degradation in the UV/PS were found to be 30% and 62% at pH 7, respectively. The contributions of SO 4 − and HO were not significantly influenced by acidic medium (pH 3–pH 7), whereas they were significantly affected by basic medium (pH 7–pH 11). The wastewater matrixes of HCO 3 − , Cl − and humic acid inhibited the DTAC elimination, whereas NO 3 − and SO 4 2− had no significant impact on its elimination. Moreover, the k obs,DTAC in the reverse osmosis influent (ROI) and reverse osmosis concentrate (ROC) were examined to be 0.04 to 0.1 min −1 and 0.02 to 0.05 min −1 , respectively, as the PS dosage increased from 18.9 to 113.4 μM. The inhibitive effects of matrix in ROI and ROC was 70% and 81%, respectively. The contribution of radical scavenging effect by matrix ROI and ROC was more significant to DTAC degradation than UV scattering effect in ROI and ROC matrices. A UV fluence of 1305 mJ·cm −2 was necessitated for complete detoxification and DTAC solution by UV/PS.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Tingting Liu,Ailing Liu,Baijun Liu,Mingyao Zhang
In this study, polydopamine (PDA) was used to modify polystyrene/nickel (PS/Ni) metal composite microspheres prepared by chemical plating technology to obtain PDA@PS/Ni microspheres with a size of 2.45 μm. These microspheres were added to a polyurethane pressure-sensitive adhesive (PSA) matrix, and each anisotropic conductive adhesive (PDA@PS/Ni-ACA) was ultimately obtained by rapid UV curing under room temperature conditions. The effects of different loading amounts on the prepared PS/Ni metal composite microspheres were investigated, and the differences in dispersibility, electrical properties, and mechanical properties between PDA@PS/Ni-ACAs and PS/Ni-ACAs were discussed. Scanning electron microscopy (SEM), thermogravimetric analysis (TGA), high resistivity measurements, and X-ray photoelectron spectroscopy (XPS) were used to characterize the metal composite microspheres and ACAs. When the PDA@PS/Ni content was 3 wt.%, the resistivity was found to be 1.3 × 10 −4 Ω cm, which is 15 orders of magnitude lower than that of pure PSAs PDA@PS, and the tensile strength was as high as 16.21 MPa. The tensile strength of pure PSA is 18.13 MPa, which is higher than that of heat-cured PSA. In addition, the conductive adhesive maintains its anisotropy when the PDA@PS/Ni content is increased to 30 wt.%. These findings will help to expand the application of ACAs in the electronic packaging industry.
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2024
·
IF 15.7
Zhounan Yu,Leilei Zhang,Yuanlong Tan,Rizheng Jing,Hongchen Cao,Caiyi Lou,Rile Ge,Junhu Wang,Aiqin Wang,Tao Zhang
Rh-P complexes have been widely used as catalysts for hydroformylation reactions. The extremely high price of Rh and its scarce reserves have prompted the exploration of the alternatives. In this study, we reported that Pt/PS-PPh 2 single-atom catalysts promoted by SnCl 2 were highly efficient and selective for the hydroformylation of higher α-alkenes. A broad scope of substrates (i.e., C 6 –C 12 ) were smoothly converted to the corresponding linear aldehydes with high yields under reaction conditions of 90–120 °C and 4–6 MPa syngas . The turnover frequency (TOF) was comparable to homogeneous Pt-Sn catalysts, and the linear/branched ratio reached as high as > 20. In addition, the catalyst could be reused with the extra addition of SnCl 2 . The promotional role of SnCl 2 was elucidated by quasi- in situ X-ray adsorption fine structure, Fourier transform infrared, and Mössbauer spectroscopy . It was discovered that SnCl 2 was transformed into Sn(dioxane)Cl 3 − species coordinated to Pt as a moderately electron-donating ligand, which, together with the phosphine group, stabilized mononuclear Pt (I) species against reduction and aggregation.
✓ 已验证
产品关联
DOI
高影响因子
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
·
2025
·
IF 3.3
Zhuang Haoran,Chu Yiwen,Wang Menglin,Liu Bo,Chen Bihua,Lin Ziyu,Li Zhenxia
The mechanisms underlying phytohormonal disruptions caused by microplastic/plasticizer contamination in agricultural systems remain poorly understood. This study systematically investigates how polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC), and di- n -octyl phthalate (DOP) alter endogenous hormone networks in agricultural systems. Integrated transcriptomic-metabolomic analyses were conducted on cucumber plants grown in hydroponic and soil systems exposed to microplastics (PE, PS, PVC) and DOP. Hormonal pathways were deciphered via gene expression profiling of key biosynthesis regulators. Microplastics and DOP primarily affected the tryptophan-dependent auxin biosynthesis pathway. Differentially expressed genes (DEGs) related to cytokinin biosynthesis were involved in the de novo synthesis pathway. All treatments primarily regulated the expression of DEGs encoding the enzymes gibberellin-20-oxidase (GA20ox), gibberellin-2-oxidase (GA2ox), and gibberellin-3-oxidase (GA3ox) to maintain the balance of active gibberellins. Changes in abscisic acid metabolite levels were linked to the expression of DEGs for abscisic aldehyde oxidase (ABA2). All treatments reduced the ethylene biosynthesis rate. Cucumber plants regulate the expression of DEGs related to phytochrome B (PHYB)-activated inhibitor 1 (CYP734A1) to maintain a balance of endogenous brassinosteroids. Jasmonic acid levels significantly increased after PE, PS, and PS + DOP treatments. Salicylic acid pathways remained unaffected despite phenylalanine ammonia lyase gene variations. PE and PS exhibit significantly stronger impacts on endogenous hormone biosynthesis in cucumber plants compared with PVC. PS with the co-presence of DOP synergistically enhancing jasmonic acid biosynthesis. Notably, smaller PS particle sizes facilitate their absorption by cucumber roots while simultaneously suppressing auxin synthesis efficiency.
研究领域:
植物科学
·
环境毒理学
·
生物信息学
·
农业化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Jipeng Guan,Yaru Yang,Bolin Tang,Xiaojun Shen,Yongjin Li
The non-spherical Janus nanosheets (JNSs) have been large-scale synthesized by grafting polystyrene (PS) chains and epoxy group on the opposite sides of JNS, denoted as epoxy-silica-PS JNS. The functional JNSs are employed as compatibilizer in the immiscible polyamide 6 (PA6)/polystyrene (PS) blend. In the melt-blending process, the JNS with epoxy group can react with carboxyl (amino)-capped PA6 and in-situ form the PA6-silica-PS JNS. Owing to the amphiphilicity and Pickering effect, the JNSs can be exclusively located at the polymeric interface and assemble into the nanosilica monolayer at 0.5 wt% loading. With increasing the JNSs loading, the size of dispersed PS phase is decreased gradually and the JNSs still maintain the single layer structure at the interface. The dynamic moduli of the blends have been effectively improved for the JNSs at the interface. This report provides an extended application of Janus nanosheets to stabilize and functionalize the polymeric interface.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Shuai Wang,Changhao Yao,Yifeng Cai,Yang Yang,Huiyuan Ma,Baojiang Jiang,Jun Ma
In this study, α-Fe 2 O 3 /rGO was smoothly synthesized by a one-step method and employed to activate persulfate (PS) for the degradation of Bisphenol A (BPA) under sunlight. During the degradation experiment, the degradation rates of BPA for PS, α-Fe 2 O 3 /8% rGO, and α-Fe 2 O 3 /8% rGO/PS were 6.6%, 47.6%, and 99.1%, respectively, indicating that the introduction of α-Fe 2 O 3 /rGO and PS significantly enhanced the photocatalytic efficiency because that photocatalytic process and PS activation coupling system not only inhibited the recombination of photo-induced carrier pairs, but also reduced the energy consumption of PS activation. Moreover, electron spin resonance spectroscopy (ESR) and trapping experiments were utilized to explore the active species generated during the reaction. The results showed that •OH and •SO 4 2- were the principal oxidative species in this reaction system. These results showed that Fe-loaded rGO has great potential for activating PS to decompose pollutants in water.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Yang Hu,Xiaoyan Lin,Datong Liu,Lili Liang,Chunxia Pang,Xunhai Pan
Oil-water emulsion separation could improve the efficiency of oil resources and reduce environmental pollution. The efficiency of oil-water separation was directly affected by the surface hydrophobicity of the membrane and its reliability and reusability. Therefore, the construction of a reliable and reusable super-hydrophobic surface was very important. In this research, the EFM with sandwich structure of PS-SiO 2 -PVAc-SiO 2 was easily fabricated by a three times liquid phase induction process and heat treatment. The PS-SiO 2 -PVAc-SiO 2 EFM was first formed by a triple liquid phase induction process, then the EFM was heat-treated, the PVAc, which is a heat-sensitive material, resulting in SiO 2 nanoparticles being welded onto PS fibers. The rolling contact angle experiment at an inclination of 8° proved that the PS-SiO 2 -PVAc-SiO 2 EFM has super-hydrophobic properties. Meanwhile, the PS-SiO 2 -PVAc-SiO 2 EFM showed excellent stability and reliability of super-hydrophobic properties after the EFM was washed or taped. In addition, the PS-SiO 2 -PVAc-SiO 2 EFM had self-cleaning function and fine oil-water separation property. The oil-water separation efficiency was over 99.50 % and the flow rate was 68 L/m 2 h. After 5 times of oil-water separation, the flux and separation efficiency did not decrease significantly, which proved that the PS-SiO 2 -PVAc-SiO 2 EFM could be reusable.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）