TOXICOLOGY AND APPLIED PHARMACOLOGY
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2022
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IF 4.46
Shiyu Zhang,Ying Liu,Ting Liu,Jie Pan,Rong Tan,Zixia Hu,Bowen Gong,Yufen Liao,Peng Luo,Qibing Zeng,Weiwei Li,Jiang Zheng
8-Epidiosbulbin E acetate (EEA), a furan-containing diterpenoid lactone , is one of main component of Dioscorea bulbifera L. (DBL). It has been reported that EEA induces severe hepatotoxicity in mice and that its hepatotoxicity is associated with metabolic activation. The present study demonstrated that exposure to EEA (50, 100 or 200 μM) induced DNA damage, including significant DNA fragmentation, increases of tail DNA and olive tail moment, H2AX phosphorylation and PARP-1 activation, in cultured mouse primary hepatocytes. Similar observation was obtained in mice administered EEA at 50, 100 or 200 mg/kg. Pre-treatment with 10 μM ketoconazole (KTC), 200 μM vitamin C (VC), or 200 μM glutathione ethyl ester (GSH-OEt) reversed the over-production of reactive oxygen species (ROS) induced by EEA and attenuated susceptibility of hepatocytes to EEA-induced cytotoxicity and DNA damage in mouse primary hepatocytes. In contrast, pre-treatment with 1.0 mM L-buthionine sulfoximine (BSO) potentiated over-production of ROS, cytotoxicity and DNA damage induced by EEA. In summary, EEA induced DNA damage in cultured primary hepatocytes and the liver of mice. ROS, possibly along with DNA alkylation , participated in the observed DNA damage.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2023
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IF 4.1
Guangzheng Ma,Zihan Zhang,Mei Chen,Yifei Zhang,Binbin Nian,Yi Hu
BACKGROUND The relative low stability, reusability and activity of enzymes made the industrial production of vitamin E succinate (VES) can only be performed with complex processes and high cost using chemical methods. To address these issues, in the present study, an ionic liquids (ILs) modification strategy was developed to improve the activity and stability of lipases in VES synthesis. RESULTS The results showed that the [1-butyl-3-methyl imidazole] [ N -acetyl- l -proline] ILs modified Candida rugosa lipase (CRL) has the highest modification degree (48.28%), activity (774 U g −1 ), thermostability and solvent tolerance in three selected modifiers. Additionally, after reaction condition optimization, the highest yield of VES can be improved to 95.18% at 45 °C for 15 h, which was significantly improved compared to some previous studies. CONCLUSION In the present study, a high-efficiency VES synthesis strategy was successfully developed via modification of lipase. Moreover, the mechanism by which ILs modification can enhance the activity and stability of lipase was investigated via both experimental and computational-aided methods. Molecular dynamics simulation suggested that ILs modification changed the geometry of Phe344 from flat to upright, which significantly reshaped and enhanced the size of substrate binding pocket of CRL. It is also agreement with our circular dichroism and fluorescence spectroscopy results, which suggested that the modification changed the secondary structure of CRL to a certain extent. The larger pocket also endowed the suitable binding pose of succinate, which made the hydrogen bonds between succinate and active site Ser209 become stronger, and thus improving the yield of VES. © 2023 Society of Chemical Industry.
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CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Dengjia Shen,Hongyang Ma,Madani Khan,Benjamin S. Hsiao
Both plastic (e.g., polyvinyl chloride (PVC)) solid waste and bacteria/heavy metal ions-containing wastewater pose a severe threat to the environment, which must be addressed simultaneously by developing highly efficient and sustainable strategies. Novel cationic PVC nanofibrous membranes with high filtration and adsorption capability were fabricated by electrospinning and surface grafting, and then employed for the removal of bacteria (e.g., E. coli ) and hexavalent chromium (Cr (VI)) ions for wastewater treatment. A cationic pendant, methylimidazolium, was covalently anchored on the surface of PVC nanofibers and verified using FTIR-ATR, NMR, and SEM measurements. As a result, the optimized cationic PVC nanofibrous membrane (PVC-MIM) exhibited remarkable filtration efficiency for E. coli (LRV ≥ 6.1) from contaminated water, and a maximum adsorption capacity of 285.7 mg/g against Cr (VI). Kinetics, isotherms, and thermodynamics of the nanofibrous PVC-MIM membrane in the adsorption process were extensively investigated. The separation mechanism was proposed based on size exclusion, charged interactions, and hydrogen bonding/coordination between the membrane and the targets. Used sewer pipes and electric cables were employed as raw materials to fabricate modified nanofibrous membranes (0.9 g) to then manufacture of a spiral-wound PVC-MIM nanofibrous membrane cartridge. The resulting system exhibited a retention rate as high as 100 % when 24 L of simulated tanning wastewater containing concentrated suspended solids, oil, ammonia–nitrogen, chloride, and other pollutants were tested for a dynamic adsorption process. Furthermore, the nanofibrous membrane cartridge could be recycled ten times while the regeneration ratio remained as high as 91.0 %.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
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IF 4.539
Ying-Ying Guan,Shao-Qi Zeng,Yin Qin,Yan Mu,Hong Liu
To overcome cancer drug resistance and to reverse tumor immunosuppression, a vitamin E-tocopheryl polyethylene glycol succinate (TPGS) decorated polymer-based drug delivery system was developed. The effects of the drug delivery system on diverse proteins involved in cell apoptosis, cancer invasion, metastasis and immunosuppression were investigated to provides a more comprehensive understanding of TPGS modified drug delivery systems. Curcumin (CUR), a hydrophobic drug, was loaded in star poly(DL-lactide) with a cholic acid core, and then decorated with TPGS shell to obtain [email protected] /star poly(DL-lactide) nanoparticles ( [email protected] ). Cholic acid cored star poly(DL-lactide) is featured by a rapid degradation rate with a surface erosion characteristic. TPGS not only reverses tumor drug resistance through downregulating P-gp expression of tumor cells but also enhances the anti-tumor efficiency through combined TPGS/CUR therapeutic actions on reversal of tumor immunosuppression mediated by CD47 and PD-L1. As compared with the CUR loaded nanoparticles without TPGS decoration, [email protected] with TPGS decoration results in significantly enhanced intracellular drug accumulation to efficiently induce cell apoptosis in drug resistant tumor cells. More importantly, [email protected] possesses considerably improved efficacy in upregulating p53 and p21 as well as downregulating β-catenin, MMP-9, Snail, CD47 and PD-L1, indicating the TPGS decorated drug delivery system inhibits tumor development and metastasis much more efficiently.
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Parasites & Vectors
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2021
·Vol. 14
·期 1
·1-11
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IF 3.876
Nwanade Chuks F.,Wang Min,Wang Tianhong,Zhang Xiaoyu,Wang Can,Yu Zhijun,Liu Jingze
Background The tick Haemaphysalis longicornis (Neumann) is a well-known vector of numerous pathogens of veterinary and medical importance. Various control strategies, including the use of synthetic pesticides, have been developed to control this tick species. However, demand for effective and safe alternative pesticides is increasing due to the adverse effects associated with the intensive and injudicious use of synthetic pesticides, which include undesirable effects on non-target species and environmental pollution. Hence, the acaricidal activity of the extract and the essential oil of Cinnamomum cassia (Chinese cinnamon) and their major components, and the underlying mechanisms of this activity, were evaluated against unfed larvae and nymphs of H . longicornis . Methods The components of the extract and essential oil of C. cassia were determined by gas chromatography-mass spectrometry, and their larvicidal and nymphicidal activity were evaluated using the larval and nymphal packet test. The underlying detoxification mechanism was elucidated by targeting in vivo esterase and monooxygenase activity, and the toxicological effect was assessed on non-target Tenebrio molitor and Harmonia axyridis by topical application in open Petri dishes. Results ( E )-cinnamaldehyde was the predominant component of the extract (50.79%) and essential oil (89.95%). The 50% lethal concentration (LC 50 ) for larvae and nymphs treated with the extract was 11.56 and 49.18 mg/mL, respectively. The essential oil, ( E )-cinnamaldehyde and fenvalerate exhibited acaricidal activity, with LC 50 values of 3.81, 3.15, and 0.14 mg/mL, respectively, against the larvae, and 21.31, 16.93, and 1.89 mg/mL, respectively, against the nymphs. ( E )-cinnamaldehyde significantly increased esterase and monooxygenase activity in both larvae and nymphs. Unlike fenvalerate, C . cassia essential oil and ( E )-cinnamaldehyde did not cause mortality of T . molitor or H . axyridis adults. Conclusions This study demonstrates that C . cassia essential oil and ( E )-cinnamaldehyde have the potential to be developed into botanical-based larvicidal and nymphicidal agents for tick control. Graphical abstract
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JOURNAL OF ELECTROCARDIOLOGY
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2017
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IF 1.514
Yaoxu Chen,Chunxia Yin,Yingying Yang,Zhuo Fan,Jinling Shang,Wen Tan
Ischemia/reperfusion (I/R) induces prolongation of QT interval and action potential duration (APD), which is a major cardiac electrical disorder in patients with arrhythmias. However, the mechanism of QT interval prolongation induced by I/R remains unclear. In the present study, we hypothesized that the rapid component of delayed rectifier potassium ( I Kr ) channel plays an important role in I/R-induced QT interval prolongation. We observed a marked attenuation of I Kr and a significant prolongation of action potential duration (APD) in a simulated I/R system with sodium dithionite (Na 2 S 2 O 4 ) in ventricular myocytes of guinea pigs. The I Kr current density was inhibited by 64% and APD increased by 87% respectively. Moreover, the inhibition of I Kr is primarily ascribed to overproduction of reactive oxygen species (ROS) by I/R, which can be partly reversed by antioxidant vitamin E (100 μmol/L). The value of I Kr tail current density increased from 0.516 ± 0.040 pA/pF in I/R to 0.939 ± 0.091 pA/pF when treated with vitamin E. Moreover, we also demonstrated that QTc interval was increased by I/R and reversed by Vitamin E in isolated guinea pig hearts. In conclusion, the inhibition of I Kr is one of the underlying mechanisms of prolongation of QT interval and APD in I/R. Vitamin E might have a benefit in coronary reperfusion therapy .
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PROCESS BIOCHEMISTRY
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2017
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IF 2.497
Shu-Ping Zou,Ji-Wei Huang,Ya-Ping Xue,Yu-Guo Zheng
1-Cyanocyclohexaneacetic acid (1-CHAA) is an important intermediate in the preparation of gabapentin. Regioselective biotransformation of 1-cyanocyclohexaneacetonitile (1-CHAN) into 1-CHAA, mediated by the immobilization of recombinant microbial nitrilase, is a novel and promising strategy for its production. In this study, a recombinant E. coli expressing Acidovorax facilis nitrilase was flocculated with polyethylenemine, followed by cross-linking with glutaraldehyde to obtain cross-linked cell aggregates (CLCAs). Under the optimum preparation conditions, the activity recovery of CLCAs reached 68%. Characterization of the CLCAs showed that the optimum pH was not affected by cross-linking, but cross-linking resulted in slightly different optimum temperature. The half-life of CLCAs at 45 °C was 266.5 h, which is 11.6-times higher than that of the free cells. Preparative-scale biotransformation of 1-CHAN to 1-CHAA at high substrate loading (up to 1.0 M) was performed using CLCAs as the biocatalyst in a 2L stirred reactor, resulting in an conversion of 91%, with a productivity of 244.5 g L −1 d −1 . The CLCAs also exhibited a high operational stability and even after 30 cycles of reaction, it retained >80% of the initial conversion.
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POLYMER
·
2024
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IF 4.1
Rui Hong,Ri-Tong Lan,Yue Ren,Lu Xu,Yun-Xiang Xu,Nouman Ali Shah,Rizwan M. Gul,Shishu Huang,Lingli Li,Jia-Zhuang Xu,Zhong-Ming Li,Ka Li
Oxidation resistance is strongly necessary for the use of crosslinked ultrahigh molecular weight polyethylene (UHMWPE) in artificial joints. The hybrid antioxidant strategy emerged as a promising alternative to the single rival that relies on vitamin E (VE). The goal of this study was to investigate the universality of the antioxidant synergy between VE and sugar alcohol (SA). Three types of SA, including D-sorbitol, maltitol and erythritol, were employed to cooperate with VE. Compared to the use of VE alone, these hybrid antioxidants generally enabled the corresponding UHMWPE composites with prolonged oxidation induction time (OIT) and increased crosslink density. The correlation between OIT and crosslink density of VE/SA-stabilized UHMWPE composites showed low attenuation. The applicability of the hybrid antioxidants to protect UHMWPE against oxidation was further demonstrated using the in vitro clinically relevant accelerated aging. Among the used SA, D-sorbitol exhibited the strongest ability to enhance the synergetic efficiency. The possible mechanism was revealed from the following aspects: the number of secondary hydroxyls per molecular mass and the molecular polarity determined via polarity parameter calculation. These findings provide significant insights for fabricating UHMWPE bearing materials with superior comprehensive performance and extended service longevity.
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Surfaces and Interfaces
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2024
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IF 5.7
Ping Lu,Zixing Gu,Zihan Zhang,Huaneng Su,Qiang Ma,Chunsheng Li,Lei Wei,Qian Xu
The all-in-one E-fueled solar flow battery system that can be recharged with liquid fuel is a new type of power system. It can continuously convert solar energy into liquid fuel without relying on external power supply equipments. However, the interface between the photoanode and the electrolyte often exhibits unsteady behavior, resulting in poor performance of the system. In this work, the {201} facets are selected and the impact of two doping treatment strategies for TiO 2 {201} photoanode on the performance of the E-fueled solar flow battery system is systematically studied through the I-t curve, linear sweep voltammetry, Mott-Schottky plot. The results show that the un-biased photocharging current of bulk-doped TiO 2 can reach 2.7 mA·cm −2 in the battery system test, only relying on the self-built electric field at the interface, and the energy conversion efficiency approaches 2.67 %. In addition, the performance is analyzed by the combination of transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and other characterizations, with the aim of distinguishing the effects of bulk- and surface-doped Mn and accurately revealing the mechanism of their influences from the outside to the inside. Finally, the optimization rules of the un-biased light charging mode of the system are explored.
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Analytical Methods
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2024
·Vol. 16
·期 28
·4865-4872
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IF 2.7
Yujie Wen,Cong Tang,Qing Shen,Shuqing Dong,Yaya Wang,Yunchun Li,Shijun Shao
Developing an effective and convenient nitrite detection method is indispensable in food safety, environmental monitoring, clinical diagnosis of diseases, and many other areas. Herein, a dicyanoisophorone derivative, TMN-NH2 with large Stokes shift and near-infrared (NIR) emission, was proposed as a ratiometric fluorescence and colorimetric dual-mode probe for the rapid determination of NO2− in acidic media, showing excellent selectivity and high sensitivity. The sensing mechanism is based on the diazotization of TMN-NH2 with NO2− and subsequent diazonium salt hydrolysis to form a hydroxyl-substituted product (TMN-OH). Under the optimized conditions of reaction and detection, a new quantitative analysis method based on TMN-NH2 was established for NO2− detection, exhibiting good linear relationships to NO2− in the range of 0.5 to 15 μM with practical detection limits of 26.6 nM and 17.6 nM for the colorimetric and fluorescent readout, respectively. The quantitative detection of NO2− in real samples demonstrated satisfactory recoveries and repeatability. Moreover, TMN-NH2 was successfully applied for monitoring NO2− in Escherichia coli by confocal fluorescence imaging.
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International Journal of Nanomedicine
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2024
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IF 6.6
Yaxu Feng,Qing Sun,Pei Liu,Wei Fan,Bing Fan
Background Enterococcus faecalis (E. faecalis) is one of the main pathogens responsible for refractory root canal infections in the teeth and shows resistance against various antibacterial managements. Effective control of E. faecalis infection is a prerequisite for successful treatment of refractory apical periodontitis. This study aimed to analyze the antibacterial activity and mechanisms of Au@Ag nanoparticles (NPs) combined with photothermal therapy (PTT) against the original and Ag+-resistant E. faecalis.Methods Au@AgNPs with optimal shell thicknesses were synthesized and characterized. The antibacterial activity of Au@AgNPs with PTT against the original or Ag+-resistant E. faecalis was evaluated, and the antibiofilm activity was tested on E. faecalis biofilm on the dentin of teeth. The potential antibacterial mechanisms of Au@AgNPs combined with PTT against E. faecalis have also been studied. Moreover, its influence on dentin microhardness and cytotoxicity was assessed.Results This study revealed that Au@AgNPs combined with PTT showed enhanced antibacterial and antibiofilm effects, no negative effects on dentin microhardness, and low cytotoxicity toward human periodontal ligament cells (hPDLCs). Moreover, Au@AgNPs combined with PTT effectively inhibited the growth of Ag+-resistant E. faecalis. Its antibacterial effects may be exerted through the release of silver ions (Ag+), destruction of the cell membrane, production of reactive oxygen species (ROS) and inhibition of adenosine triphosphate (ATP) production. Hyperthermia generated by Au@AgNPs with PTT reduced membrane fluidity and enhanced Ag+ sensitivity by downregulating fabF expression. The upregulated expression of heat shock genes demonstrated that the Ag+ released from Au@AgNPs compromised the heat adaptation of E. faecalis.Conclusion PTT significantly enhanced Ag+ sensitivity of the original and Ag+-resistant E. faecalis. Au@AgNPs combined with PTT may have the potential to be developed as a new antibacterial agent to control E. faecalis infections in teeth.
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FOOD RESEARCH INTERNATIONAL
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2024
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IF 7
Xiaoli Tan,Xiaobing Jiang,Okwong Oketch Reymick,Chen Zhu,Nengguo Tao
Stem-end rot caused by Neofusicoccum parvum is among the most detrimental diseases affecting postharvest mangoes. The present investigation utilized ( E )-2-octenal to manage N. parvum infections, elucidating its mechanism of action. The findings revealed that ( E )-2-octenal exhibited outstanding antimicrobial potency against N. parvum in vitro within the concentration range of 0.4–1.6 µL/mL. Additionally, ( E )-2-octenal significantly compromised the cell membrane integrity and mitochondrial energy metabolism of N. parvum , evidenced by dramatically increased intracellular material leakage and ROS levels, along with reduced mitochondrial membrane potential, ATP, and energy charge. Further experiments showed noteworthy effects on the tricarboxylic acid cycle (TCA) cycle and the key enzyme activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH). Molecular docking revealed hydrogen bonding between ( E )-2-octenal’s aldehyde group and SDH (Trp-307) and MDH (Gly-101), indicating direct targeting of these enzymes for inhibition. To enhance the practical application of ( E )-2-octenal, we developed an aerogel-loaded ( E )-2-octenal material (ALO) that exhibited superior antimicrobial efficacy in vitro . In vivo , ALO effectively controlled mango stem-end rot, with optimal efficacy at 20 µL/L. This concentration also delayed the natural disease of mango without degrading fruit quality. According to these findings, ( E )-2-octenal is a promising preservative against postharvest mango infections, potentially by impeding cellular energy metabolism through direct interaction with SDH and MDH within the TCA pathway, culminating in mitochondrial dysfunction and cell membrane damage.
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