FOOD CHEMISTRY
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2020
·
IF 6.306
Yu Lu,Cheng Li,Jie Liu,Wenqi Huang,Yuerong Yang,Yue Jia,Tingting Liu,Linjuan Huang,Zhongfu Wang
Goat milk oligosaccharides represent an unexplored multi-functional ingredient for the dairy industry. Here, we qualitatively and quantitatively compared the N/O -glycome at different lactation stages via online hydrophilic interaction chromatography-tandem mass spectrometry. Complex N -glycans and high mannose N -glycans constituted 82.1% and 17.9% of the glycan pool, respectively. N -glycans with isomers containing non-bisected antenna complex structures accounted for 30.8%. N -glycans modified with Neu5Ac, Neu5Gc and fucosylated were 3.7%, 5.3% and 35.3%. The triantennary trifucosylated complex N -glycan (H5N5F3) was reported for the first time. A comparison between colostrum and mature milk revealed a 1.20-fold decrease in total N -glycans and 1.66-fold decrease in fucosylation with ongoing lactation, echoing the trend in human milk. Similarly, Neu5Ac- and Neu5Gc-modified sialylation decreased by 1.69 and 3.62 times, respectively. In the O -glycome, 46.2% of structures were O -linked core 1, 23.1% were O -linked core 2, 7.7% were O -linked core 3 and core 4. As lactation progressed, overall O -glycans content decreased by 1.26-fold. Unlike human milk, Neu5Ac- and Neu5Gc-modified sialylation increased by 4.4 and 2 times, respectively. These findings will facilitate research on the structure-function relationship of goat milk oligosaccharides and the development of formula food targeting different age groups.
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Journal of Flow Chemistry
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2021
·Vol. 11
·期 2
·171-179
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IF 2.786
Lan Zhou,Lu Yangcheng
The matching of the micro-dispersion method and the reaction kinetics is of the utmost importance for the fast and strongly exothermic nitration reaction. Taken as an example to discuss this issue, a synthesis of 3,4-dichloronitrobenzene via the nitration of o -dichlorobenzene with mixed acid of nitric acid and sulfuric acid was conducted in continuous-flow reactors in this work. Through combining the adiabatic reaction environment, the use of a micropacked-bed reactor, and introducing partial product circulation, it successfully achieved the matching of the micro-dispersion state and the reaction kinetic characteristics. The good two-phase dispersion state can be maintained throughout the micropacked-bed reactor and the reaction can be completed within 5 s with above 89% selectivity under adiabatic condition. Furthermore, the reaction activation energy was conveniently obtained by processing the temperature distribution data, providing a fundamental to realize a reliable design of the nitration reaction process with high efficiency and inherent safety. Graphical abstract
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MOLECULES
·
2020
·Vol. 25
·期 9
·2109
·
IF 3.267
Changzhou Chen,Peng Liu,Haihong Xia,Minghao Zhou,Jiaping Zhao,Brajendra K. Sharma,Jianchun Jiang
It is of great importance to explore the selective hydrogenolysis of β-O-4 linkages, which account for 45–60% of all linkages in native lignin, to produce valued-added chemicals and fuels from biomass employing UV light as catalyst. TiO2exhibited satisfactory catalytic performances in various photochemical reactions, due to its versatile advantages involving high catalytic activity, low cost and non-toxicity. In this work, 20 wt.% Ni/TiO2and oxidant PCC (Pyridinium chlorochromate) were employed to promote the cleavage of β-O-4 alcohol to obtain high value chemicals under UV irradiation at room temperature. The Ni/TiO2photocatalyst can be magnetically recovered and efficiently reused in the following four consecutive recycling tests in the cleavage of β-O-4 ether bond in lignin. Mechanism studies suggested that the oxidation of β-O-4 alcohol to β-O-4 ketone by oxidant PCC first occurred during the reaction, and was followed by the photocatalysis of the obtained β-O-4 ketone to corresponding acetophenone and phenol derivates. Furthermore, the system was tested on a variety of lignin model substrates containing β-O-4 linkage for the generation of fragmentation products in good to excellent results.Keywords:lignin;β-O-4;photocatalyst;Ni/TiO2;oxidant PCC
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Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 27
·9337-9344
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IF 7.059
Aili Wang,Lili Zhao,Hui Liu,Ziqian Zhou,Chengbo Li,Yong Xiang,Weijia Zhou,Feng Hao
The development of low-dimensional non-precious metal catalysts for the hydrogen evolution reaction (HER) is highly desired for clean energy utilization. Herein, we have reported a facile synthesis protocol of Cu–Mo–O (CMO) nanowires and nanosheets on Cu foam (CMOW/CF and CMOS/CF) through a one-step reaction of Cu nanowires with (NH4)6Mo7O24 solution directly under mild conditions. The final product morphology has been tuned by mechanical stirring. The as-synthesized CMOS/CF exhibits a low operating overpotential of 112 mV at a current density of 10 mA cm−2 with a high electrochemical active area of 25.1 mF cm−2 and excellent long-term stability in the 1 M KOH electrolyte. Moreover, by using the CMOS/CF as cathodic and anodic electocatalysts, a two-electrode electrolyzer capable of driving 10 mA cm−2 at a cell voltage of 1.69 V was achieved, along with promising stability. The high performance of the CMOS/CF can be attributed to higher electrochemical area and more Mo catalytic sites induced by mechanical stirring. This work provides a facile method for the synthesis and catalytic site regulation through a simple method of low-dimensional nanomaterials for the HER with relatively high electrochemical active area and catalytic activity.
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Yuting Li,Qiaoling Huang,Xiangying Yu,Yujia Liu,Lin Li,Bing Li,Xia Zhang,Sui Chen,Zhan Liu,Xianglong Zhao,Jingjing Ma
The reaction of N ε -(carboxymethyl) lysine (CML) with eight kinds of non-flavonoid o -benzoquinones and five kinds of flavonoid o -benzoquinones were investigated by cyclic voltammetry at pH 5.0, 7.0 and 8.0 and scan rate of 10, 50 and 100 mV/s. The reactivity of o -benzoquinones towards CML is weakened by the electron-donating substituent and strengthened by the electron-withdrawing substituent on the o -benzoquinone rings. The steric hindrance of the substituents on o -benzoquinone rings also weakens the quinone reactivity. Reaction of 4-methylbenzoquinone with CML (38.0 ± 1.3%) was found to be faster than that with l -lysine (31.3 ± 1.5%) and Nα-acetyl- l -lysine (14.5 ± 0.1%) but slower than that with l -cysteine (≥100.0%) and Nα-acetyl- l -cysteine (≥100.0%) at pH 7.0 and scan rate of 10 mV/s. Products obtained by the reaction of CML with o -benzoquinones were found to include a CML-quinone adduct according to the cyclic voltammetry and UPLC-QTOF-MS/MS analysis.
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LASERS IN MEDICAL SCIENCE
·
2019
·Vol. 34
·期 8
·1535-1545
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IF 2.076
Zheng Yixin,Yu Endian,Weng Qi,Zhou Leilei,Li Qingyong
Antibiotics and photodynamic therapy (PDT) are widely employed in curing acne. However, antibiotics as an effective treatment would lead to bacterial resistance and severe side effects. In this study, we aimed to develop a novel TBO hydrogel, which could prolong the retention time of photosensitizer (TBO) at the lesion site and improve therapeutic effect. In vitro antibacterial experiments (against Staphylococcus aureus and Escherichia coli ), the response surface methodology was used to optimize the formulation of TBO hydrogel. The results indicated that the optimal formulation was 0.5% ( v / v ) carbomer, 0.01 mg/mL TBO, 0.5% ( v / v ) ethanol concentration, 0.5% ( v / v ) Tween 80, the mass ratio of NaOH to carbomer of 0.4 ( w / w ). The TBO hydrogel formulation showed the strong antibacterial activity for Propionibacterium acnes . The stability, pH, and antibacterial activity of TBO hydrogel did not significantly change under 4 °C, 25 °C, and 40 °C during 6-week storage. Furthermore, TBO combined with carbomer hydrogel showed the 51.28% (4 h) and 69.80% (24 h) release. In summary, the hydrogel TBO might be a vital therapeutic strategy to promote the PDT applied in the topical therapy of acne.
A TBO hydrogel for photodynamic therapy in the treatment of acne
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IUBMB LIFE
·
2018
·Vol. 71
·期 3
·330-339
·
IF 3.236
Qing Wang,Maikun Teng,Xu Li
Catechol- O -methyltransferase (COMT 1 ) catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to various catechol substrates. COMTs play vital roles in physiological processes in animals, plants, and fungi, as well as bacteria, and have essential application values in industry. sp COMT is a probable COMT from Schizosaccharomyces pombe . It has an extraordinary intracellular distribution different from other homologs and would thus be predicted to perform a distinct physiological function. In this report, recombinant sp COMT was purified and kinetically characterized for the first time. The enzymology assays indicate that sp COMT is a metal-dependent enzyme and belongs to class I OMTs. In addition, the crystal structures of apo- sp COMT and SAM-complexed sp COMT were also presented, revealing that sp COMT possesses a conserved SAM-binding site and Mg 2+ pocket, but a distinct substrate pocket was not present in homologs. The mutagenesis ITC analysis revealed the SAM recognition characteristics of sp COMT. Based on all of the above findings, we speculated about the putative substrates’ characteristics and the substrate recognition mechanisms of sp COMT. This work will help in elucidating the physiological functions of sp COMT in S. pombe . © 2018 IUBMB Life, 71(3):330–339, 2019
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Chinese Herbal Medicines
·
2018
·
IF 0
Ding Liu,Yan-peng Li,Hong-xiu Shen,Yang Li,Jun He,Qi-zhi Zhang,Yun-mei Liu
Objective To design and synthesize a series of chrysin derivatives and evaluate the antitumor activities with MTT assay , so as to investigate molecular structure-activity relationship with molecular docking . Methods Target products were synthesized with high yield by substitution reaction, hydrolysis reaction, esterification reaction, and saponification reaction in sequence, and activities of all compounds were evaluated with human gastric carcinoma cell lines MGC-803 and human breast carcinoma cell lines MCF-7 through standard MTT assay. Molecular docking results were calculated with Surflex Geom X programme of Sybyl X-2.0 version workstation. Results 7- O -amino acids chrysin derivatives 6a – 6l were synthesized and their inhibitory effects were evaluated by comparing the material chrysin with positive control drug 5-fluorouracil (5-FU). Among these derivatives, compound 5b (IC 50 = 24.50 ± 2.26 µmol/L), 5k (IC 50 = 24.30 ± 2.19 µmol/L), and 6f (IC 50 = 24.61 ± 2.01 µmol/L) showed better inhibitory activities against MGC-803 cell lines, and compound 5g (IC 50 = 13.15 ± 1.73 µmol/L) and 5j (IC 50 = 12.34 ± 1.25 µmol/L) showed better inhibitory activities against MCF-7 cell lines than chrysin and 5-FU. Molecular docking scores showed a credible consistency compared with MTT results. Conclusion Compounds 5b, 5d, 5g, 5j, 5k , and 6f showed good antiproliferative effects on specific tumor cells, and compound 5g should be researched further when according to molecular docking.
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RSC Advances
·
2019
·Vol. 9
·期 26
·14692-14700
·
IF 3.049
Fangfang Peng,Yangchuan Ke,Shichao Lu,Yi Zhao,Xu Hu,Qingchun Deng
A series of anionic amphiphilic random copolymers with sodium p-styrene sulfonate and dodecyl methacrylate side chains were synthesized via free radical polymerization and their properties in the formation and stabilization of nano-emulsions were investigated. Using poly(sodium p-styrene sulfonate)-ran-poly(dodecyl methacrylate) and Brij 30 as a stabilizer to prepare nanoemulsions, we obtained small droplet size and unimodal distribution nanoemulsions by a low-energy phase inversion composition (PIC) method. The p(SSS)-ran-p(LMA)-Brij 30 co-stabilized nanoemulsions show extraordinary long-term stability and heat resistance, there were almost no variations of droplet size after storing for 35 days and no phase inversion occurred when heating the temperature up to 90 °C. The influence of salinity on the properties of the nanoemulsions was also discussed.
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KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2014
·Vol. 31
·期 12
·2192-2197
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IF 1.241
Anjum Muhammad Naveed,Zia Khalid Mahmood,Zhu Lihua,Haroon-ur-Rashid,Ahmad Mirza Nadeem,Zuber Mohammad,Tang Heqing
Self-assembled hollow microspheres poly( o -chloroaniline) (POC) have been synthesized by simple oxidative polymerization of o -chloroaniline using camphor-sulfonic acid (CSA) as dopant acid and ammonium persulfate (APS) as oxidant. The POC microspheres were characterized by means of FTIR, XRD and SEM analysis. Adsorption characteristics of POC spheres were studied by using methyl orange (MO) as adsorbate. It was found that adsorption of MO by POC was better described by pseudo-second-order kinetics than any other kinetic model, such as the pseudo-first-order and intra-particle diffusion models. Langmuir adsorption isotherm model was the best to fit experimental data. The results showed that hollow microspheres of POC can be used as novel and low-cost adsorbent for removal of organic dye from waste water.
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CARBOHYDRATE POLYMERS
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2013
·
IF 3.479
Bo Lu,Dan Huang,Hua Zheng,Zhijun Huang,Peihu Xu,Haixing Xu,Yihua Yin,Xia Liu,Dan Li,Xueqiong Zhang
A series of melphalan-O-carboxymethyl chitosan (Mel-OCM-chitosan) conjugates with different spacers were prepared and structurally characterized. All conjugates showed satisfactory water-solubility (160-217 times of Mel solubility). In vitro drug release behaviors by both chemical and enzymatic hydrolysis were investigated. The prodrugs released Mel rapidly within papain and lysosomal enzymes of about 40–75%, while released only about 4–5% in buffer and plasma, which suggested that the conjugates have good plasma stability and the hydrolysis in both papain and lysosomes occurs mostly via enzymolysis. It was found that the spacers have important effect on the drug content, water solubility, drug release properties and cytotoxicity of Mel-OCM-chitosan conjugates. Cytotoxicity studies by MTT assay demonstrated that these conjugates had 52–70% of cytotoxicity against RPMI8226 cells in vitro as compared with free Mel, indicating the conjugates did not lose anti-cancer activity of Mel. Overall these studies indicated Mel-OCM-chitosan conjugates as potential prodrugs for cancer treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2014
·
IF 3.096
Weiqiang Huo,Weixin Zhang,Wei Wang,Xiaohua Zhou
Two types of microcapsules (MCs) were prepared by the emulsion cross-linking method, where biguanidino chitosan (BGCS)and O -carboxymethyl chitosan ( O -CMCS) served as the wall materials, and the antibacterial agent 2,4-diamino-6-(2-pyridyl)-1,3,5-triazine (PyTNH) served as a model water-soluble drug. The physicochemical performance of the MCs and their drug release behavior were investigated by Fourier transform infrared spectroscopy, thermogravimetric analysis/derivative thermogravimetric analysis, scanning electron microscopy, and swelling and in vitro drug release studies of the two MCs with unmodified chitosan-MCs (CS-MCs) used as the control. The results indicated that the degree of cross-linking, encapsulation efficiency, and thermal stability of the shell wall of the BGCS-microcapsules (BGCS-MCs) were much higher than those of the control and the O -CMCS-microcapsules (CMCS-MCs), owing to the reduction of steric hindrance and development of the conjugation effect in the cross-linking process. Studies on the swelling and in vitro drug-release behavior revealed a sustained release effect of the BGCS-MCs. Moreover, the CMCS-MCs were found to exhibit a pH-dependent drug release behavior, which can be attributed to the successive formation of H-bonds and repulsive forces with the change in the pH of the medium. Based on these results, the swelling-release models and the drug release kinetics of BGCS-MCs and CMCS-MCs are proposed.
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