JOURNAL OF BIOMATERIALS APPLICATIONS
·
2025
·
IF 2.5
Yang Yang,Hua Xie,Bi Zhou,Yuling Liu,Jiqing Hao
A single drug therapeutic approach fails to sufficiently eliminate tumors in clinics, owing to the tumoral complexity and heterogeneity. The integration of chemotherapy with novel treatment modalities offers a promising synergistic strategy for enhancing therapeutic outcomes. Herein, we successfully develop pH-triggered dynamic organic nanocomposite hydrogels for enhanced local tumor combination therapy, which are prepared through incorporation of Cu2-xSe nanoparticles (NPs) and lipophilic mixture (OPC) including orthoester compound, polycaprolactone and carboplatin into imine-crosslinked polysaccharide-based hydrogels. The dynamic imine linkages endow the hydrogels with favorable injectability and self-adaptability as well as tumoral extracellular pH-triggered degradation. OPC can enhance drug escape from hydrogels, tumor penetration and pH-responsive prolonged and sustained release of the drug. Cu2-xSe NPs endow the hydrogels with photothermal therapy (PTT), photodynamic therapy (PDT) and chemo-dynamic therapy (CDT). These superior physicochemical properties result in significant tumor growth inhibition while decreasing side effect on normal tissues. Therefore, the pH-responsive dynamic organic nanocomposite hydrogels have great potential for local tumor therapy in clinics.
研究领域:
肿瘤学
·
生物材料
·
纳米医学
·
药物递送
·
转化医学
·
光热治疗
·
化学动力学治疗
✓ 已验证
DOI
PubMed
高影响因子
STAR Protocols
·
2020
·
IF 0
Yi Li,Keke Liu,Ying Zhou,Jing Yang,Peng Zou
Summary Alk-Ph is a clickable APEX2 substrate developed for spatially restricted protein/RNA labeling in intact yeast cells. Alk-Ph is more water soluble and cell wall permeable than biotin-phenol substrate, allowing more efficient profiling of the subcellular proteome in microorganisms. We describe the protocol for Alk-Ph probe synthesis, APEX2 expression, and protein/RNA labeling in yeast and the workflow for quantitative proteomic experiments and data analysis. Using the yeast mitochondria as an example, we provide guidelines to achieve high-resolution mapping of subcellular yeast proteome and transcriptome. For complete details on the use and execution of this protocol, please refer to Li et al. (2020) .
研究领域:
分子生物学
·
蛋白质组学
·
细胞生物学
·
生物化学
✓ 已验证
DOI
PubMed
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 26
·4594-4604
·
IF 4.872
Liangliang Dai,Qingfeng Zhang,Xinkun Shen,Qiang Sun,Caiyun Mu,Hao Gu,Kaiyong Cai
A pH-responsive drug delivery system based on hollow mesoporous silica nanoparticles (HMSNs) was fabricated for targeted tumor therapy by using hydrazone bonds as pH-sensitive linkers and hyaluronic acid (HA) molecules as both blocking and targeting agents. HMSNs were synthesized with good dispersion and dimensions of around 88 nm. Detailed material characterisation suggested that the drug delivery system was successfully constructed. It displayed a fast pH stimulus response for controlled drug release in vitro. Besides, systematic biological investigations revealed that the drug delivery system had good biocompatibility, which could effectively target tumor cells and deliver therapeutic cargo to induce tumor cell apoptosis in vitro and suppression of tumor growth in vivo. This study reports a promising drug delivery system for potential clinical application against tumor therapy.
研究领域:
纳米技术
·
药剂学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jian Cheng,Hang Zhou,Ziran Yu,Hai Ren,Xiaoyu Sun,Huayu Wu,Shikuo Li
The smart therapy is a big challenge to the wound healing caused by bacterial infections for avoiding the antibiotic abuse. Herein, a visible light driven polyacrylamide/trehalose/Cu-Tetrakis (4-carboxyphenyl) porphyrin/bromothymol blue (CuTCPP) hydrogel with dual-sensing capabilities and on demand photodynamic therapy was synthesized by an in-situ polymerization method. The CuTCPP in the hydrogel can harvest the light for the generation of reactive oxygen species (ROS). The wound infection can be real-time monitor not only by the on-site pH variations, but also the electrophysiological signals. The sterilization efficacy of the CuTCPP hydrogel was achieved as high as 10 7 CFU mL −1 under visible light irradiation (λ ≥ 420 nm, 30 min). Remarkably, in vivo infection on mouse displayed that the photodynamic effect of CuTCPP hydrogel was much better than that of the control experimental. Importantly, the release of ROS during the wound healing can be adjusted on demand. And the hydrogel displayed good tissue adhesion, self-repairing and good biocompatibility as well. The photodynamic mechanism was investigated via the fluorescence, ESR spectra and the charge density difference analysis. Such intelligent hydrogel provided important insights for the development of precise theragnostic platforms to advanced interventions in critical wounds.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 89
·85885-85890
·
IF 3.289
Dongjian Shi,Xiaodong Zhang,Zhaokun Yang,Shirong Liu,Mingqing Chen
This paper presents fabrication of polyacrylamide (PAM) and PAM/poly(methacrylic acid) (PMAA) inverse opal photonic crystal hydrogels (IOHs) based on poly(styrene-acrylic acid) (PSA) template by a “Sandwich” method. SEM images showed the obtained IOHs had a three-dimensional ordered structure and extremely uniform pore size without over-layers and defects. The IOHs exhibited brilliant structural color by controlling the PSA particle size, MAA and cross-linker compositions. The color and Bragg diffraction peak of the IOHs responded to external stimuli such as pH, methanol and ethanol. The reflectance was red-shifted in low pH and blue-shifted in high pH and in the presence of methanol and ethanol. The effects of the MAA and cross-linker composition on the response properties of the IOHs were investigated. The PMAA in the IOHs provided –COOH groups that are apt to form hydrogen bonds with water, enhancing the response sensitivity.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Wenfeng Yan,Junmao Li,Wei Long,Yulin Feng,Mingzhen He,Weifeng Zhu,Meiying Liu,Hui Ouyang,Xiaoyong Zhang,Yen Wei
In this paper, a simple method that relied on direct chain transfer free radical polymerization was reported to synthesize MSNs based polymeric composites (named as MCM-41-SH-poly (PEGMA- co -VPA)) using poly (ethylene glycol) monomethyl ether (PEGMA) and 4-vinyl phenylboric acid (VPA) as monomers. The utilization of MCM-41-SH-poly (PEGMA- co -VPA) to load and delivery curcumin (CUR) was also evaluated in detail. The structure, properties, drug loading and release behaviors were evidenced by various characterization techniques. The results from biological studies have shown that CUR can be effectively loaded on MCM-41-SH-poly (PEGMA- co -VPA) with high drug loading rate through formation of borate bond between CUR and VPA. More importantly, resultant drug-loading complexes displayed excellent water dispersibility and CUR could be released from complexes with pH responsiveness. Taken together, we have developed a simple method for surface modification MCM-41 and resultant composites could display improved properties for intracellular controlled drug delivery applications.
研究领域:
材料科学
·
纳米医学
·
药物递送系统
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Qi Zhang,Lei Wang,Baiyang Chen,Yi Chen,Jun Ma
Understanding ultraviolet photolysis induced by low pressure mercury lamp that emits both 254 nm ultraviolet (UV 254 ) and 185 nm vacuum UV (VUV 185 ) is currently challenging due to the copresence of multiple direct and indirect photochemical processes involving a series of highly-reactive radicals. Herein we examined the formation and transformation of H 2 O 2 in water, which is both a precursor and a product of radicals, under various pH and dissolved oxygen (DO) conditions. The trends show that H 2 O 2 increased rapidly at early stage and then remained steady in DO-rich water or declined somewhat in DO-poor water, ultimately leading to higher steady-state H 2 O 2 in DO-rich water. The maximum H 2 O 2 contents nonetheless were similar among waters with different DO, suggesting that H 2 O 2 in this system was mostly generated by hydroxyl radical ( OH) recombination, which is an oxygen-independent H 2 O 2 formation pathway, rather than by reduced oxygen via hydrogen atom (H ) or hydrated electron (e aq − ), which is an oxygen-dependent pathway. Increasing pH (from 6.3 to 10.0) or bicarbonate dosage dramatically decreased H 2 O 2 formation too. Mathematically, the fates of H 2 O 2 as a function of pH, DO, and time were well modeled (R 2 ≥ 0.92), in which the rates of H 2 O 2 formation and destruction were greater in DO-poor water than those in DO-rich water. In addition, we found that the steady-state concentrations of OH used for degradation of p-chlorobenzoic acid, an OH probe, correlated well with the OH levels used for H 2 O 2 formation (R 2 = 0.98). These results hence may help better understand the UV/VUV process via H 2 O 2 evolutions.
研究领域:
环境科学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 22
·
IF 4.6
Xiaoxu Zhang,Chengguo Liu,Xiaoqin Yang,Qian Jiang,Can Liu,Ping Zhao
This study presents the development of a starch-based smart film enhanced with carbon dots (CDs), which exhibits high pH sensitivity for the rapid assessment of liquid food quality. CDs were synthesized from ellagic acid and urea as precursors and incorporated into amylopectin (CA) and whole-component starch (CS) matrices, yielding a range of starch-based/CDs composite films. Structural analyses indicated that hydrogen bonding interactions between the CDs and starch molecules not only provided a nano-plasticizing effect-significantly improving film flexibility, as evidenced by a 29.6-fold increase in the elongation at break of the CS/CDs-2 film—but also slightly enhanced the crystallinity by acting as a nucleating agent. The incorporation of CDs markedly improved the electrochemical properties of the films, reducing the electrical impedance by several orders of magnitude and imparting intense blue fluorescence. The fluorescence intensity and current response of the composite film were found to be highly sensitive to pH variations. A strong linear positive correlation (R2> 0.97) was observed between the current output and pH over the range of 2 to 7. In a simulated orange juice spoilage test, the film effectively distinguished between fresh (pH = 3.38) and spoiled (pH = 2.68) samples based on current signal differences, demonstrating its practical utility. This work offers a novel approach for designing low-cost, biodegradable sensing materials for smart packaging, with promising potential in real-time food freshness monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2025
·Vol. 83
·期 1
·31
·
IF 4
Huang Ruoxi,Yi Yaxin,Zhang Shiyu,Wang Jiaxin,Yuan Haikuan,Lu Jie
A stimulus-responsive drug delivery system holds promise for reducing adverse effects and enhancing the bioavailability of chemotherapeutic agents. In this study, amino-terminated dextran was utilized as a macromolecular initiator to prepare a range of amphiphilic dextran-b-poly(ε-benzyloxycarbonyl- l -lysine) (Dex-b-PZLL) copolymers with varying hydrophobic segment lengths via ring-opening polymerization (ROP). Folic acid (FA) was further grafted onto the dextran side chains via esterification (FA-Dex-b-PZLL), offering the potential tumor-specific recognition. The resulting copolymers were able to self-assemble into stable spherical micelles via dialysis and efficiently encapsulated the hydrophobic drug letrozole (LTZ). A systematic assessment was conducted on the drug encapsulation efficiency/loading capacity, drug release performance in vitro, and biocompatibility of the copolymers under aqueous conditions, indicating that these properties of the FA-Dex-b-PZLL micelles mainly depended on the copolymer composition. The optimized micelles, with a balanced hydrophilic/hydrophobic ratio, achieved a drug loading capacity with approximately 8.49% and an encapsulation efficiency with about 46.39%. Moreover, LTZ/FA-Dex-b-PZLL micelles exhibited pH-responsive release, with cumulative release rates within 72 h of 20.74%, 27.14% and 62.96% in phosphate buffer at pH 7.4, 6.5 and 4.5, respectively, indicating the accelerated release under acidic conditions. In addition, the micelles showed good cytocompatibility according to the CCK-8 assay. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Hong Huang,Fangyuan Dong,Yang Tian
Intracellular pH undertakes critical functions in the formation of a proton gradient and electrochemical potential that drives the adenosine triphosphate synthesis. It is also involved in various metabolic processes occurring in mitochondria, such as the generation of reactive oxygen species, calcium regulation, as well as the triggering of cell proliferation and apoptosis. Meanwhile, the aberrant accumulation of O2•– within mitochondria is frequently intertwined with mitochondrial dysfunction and disease development. To disentangle the complicated inter-relationship between pH and O2•– in the signal transduction and homeostasis in mitochondria, herein we developed a mitochondria-targeted single fluorescent probe for simultaneous sensing and imaging of pH and O2•– in mitochondria. CdSe/ZnS quantum dots encapsulated in silica shell was designed as an inner reference element for providing a built-in correction, as well as employed as a carrier to assemble the responsive elements for O2•– and pH, together with mitochondria-targeted molecule. The developed nanosensor demonstrated high accuracy and selectivity for pH and O2•– sensing, against other ROS, metal ions, and amino acids. The remarkable analytical performance of the present nanosensor, as well as good biocompatibility, established an accurate and selective approach for real-time imaging and biosensing of O2•– and pH in mitochondria of live cells.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2016
·Vol. 18
·期 47
·32266-32271
·
IF 4.449
Bo Yu,Yunyun Huang,Jun Zhou,Tuan Guo,Bai-Ou Guan
A method based on a fiber-optic interferometer to study the pH-dependent interaction between graphene oxide and single-stranded DNA chains is carried out with the assistance of a scanning electron microscope and a confocal laser scanning microscope. The various wavelength shifts of the interferometric fringes of the transmission spectrum reveal the different strengths of interaction between graphene oxide and single-stranded DNA in various pH environments. The present work demonstrates the feasibility of optical fibers for studying the interaction between graphene oxide and biomolecules. It provides a potential means to understand the intermolecular interactions. This technique might effectively supplement the existing tools.
研究领域:
光学
·
生物分子相互作用
·
材料科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Qizhan Zhang,Minghua Zhou,Xuedong Du,Pei Su,Wenyang Fu,Ge Song
To simultaneously improve the H 2 O 2 electrosynthesis, Fe(II) regeneration and pH application range in EF process, a highly efficient dual-cathode electro-Fenton (EF) process using easily available Fe(III) as catalysts for the treatment of organic wastewater at a wide pH range was first proposed, in which natural air diffusion electrode (NADE) was designed as one cathode for H 2 O 2 production without aeration and the modified carbon felt (MCF) was acted as the other cathode mainly for Fe(II) regeneration. Nitric acid modification significantly enhanced the electrochemical properties of CF and introduced more oxygen-containing functional groups. The Fe(II) regeneration rate of MCF with higher electroactive surface area was 4.7 times that of CF cathode at pH 7. It was found that as the homogeneous Fe(III) was hydrolyzed into solid hydroxides, the accompanying H + decreased the solution pH and MCF not only accelerated the dissolved Fe(III)/Fe(II) cycle but also reduced the adsorbed solid iron to ≡Fe(II) on the cathode surface. This simultaneously promoted the homogeneous and heterogeneous Fenton reactions and enhanced the efficiency of EF degradation process. For bronopol, 2,4-dichlorophenoxyacetic acid, sulfamethazine and phenol (100 mg L −1 ), the process had TOC removal of 48%−72% within 60 min with the corresponding mineralization current efficiency of 16.5%−56.2%, and the electrical energy consumption <0.225 kWh (gTOC) −1 , which all greatly advantaged over that of the conventional EF. Therefore, this process is efficient and low-cost, being very promising for the treatment of organic wastewater.
研究领域:
环境科学
·
污水处理
·
电化学技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）