JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 22
·9425-9435
·
IF 3.553
Guo Yufei,Wang Li,Xu Lijuan,Peng Canwei,Song Yonghai
A flexible ascorbic acid (AA)-imprinted poly(o-phenylenediamine) (ploy(o-PD))/zeolite imidazole frameworks-67 (ZIF-67)/carbon cloth (CC) electrode was prepared for electrochemical sensing AA. The flexible AA-imprinted poly(o-PD)/ZIF-67/CC electrode was prepared by growing ZIF-67 on flexible CC, electropolymerization of o-PD with AA on ZIF-67/CC and eluting template molecules AA. The results showed that the spherical ZIF-67 crystals were arranged evenly and firmly on the fibers of CC surface. The diameter of spherical ZIF-67 crystals was approximately 500–600 nm, and the spherical ZIF-67 crystals catalyzed the oxidation of AA well. A layer of poly(o-PD) with AA-imprinted pores was covered on the ZIF-67/CC electrode, so the AA-imprinted poly(o-PD)/ZIF-67/CC electrode exhibited better selectivity toward AA detection and good stability. The molecularly imprinted sensor exhibits high sensitivity, and the sensitivity could reach 959.9 μA/mM −1 cm −2 , the detection limit was 0.019 μM, and the concentration range of AA was 0.057 μM–11.4 0 mM. The AA-imprinted poly(o-PD)/ZIF-67/CC electrode also showed higher stability and repeatability. The results proved that the spherical ZIF-67 crystals were good electrode materials for electrochemical AA biosensors.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Shuqi Zhou,Ying Gan,Liubing Kong,Jiadi Sun,Tao Liang,Xinyi Wang,Hao Wan,Ping Wang
Adenosine has received great attentions acting as a potential biomarker for monitoring lung cancer. Most of the reported studies for adenosine detection require large instruments and complicated procedures. Herein, a sensitive, rapid and in-situ colorimetric aptasensor was developed for adenosine detection. Moreover, a homemade biomimetic electronic-eye (E-eye) was established and utilized as a portable in-time detection equipment. The entire measurement can be completed within 20 min, including the combination of aptamer with adenosine or AuNPs and the detection of adenosine. Four different kinds of aptamer were compared and the results showed that the AuNPs-aptamer-biotin system was the most stable and with the widest detection range of 5.0 μM–60.0 μM and the lowest LOD of 0.17 μM. Moreover, the artificial urine samples were also tested with a linear range from 5.0 to 50.0 μM and a LOD of 0.48 μM. The results validated that the aptasensor together with the E-eye can be a promising platform for adenosine detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 8
·3009-3016
·
IF 3.978
Dechan Lu,Min Fan,Rongyuan Cai,Zufang Huang,Ruiyun You,Luqiang Huang,Shangyuan Feng,Yudong Lu
Dopamine (DA) is one of the catecholamine neurotransmitters used for the treatment of neural disorders. In this study, a novel sensor based on surface-enhanced Raman scattering (SERS) with dual molecule-recognition for ultrasensitive detection of DA was presented, with a limit of detection (LOD) of 40 fM, without any pretreatment of clinical samples. To realize the sensitive and selective detection of DA in complex samples, the nanoporous silver film (AgNF) surfaces were functionalized with mercaptopropionic acid (MPA) to accurately capture DA, while silver nanocubes (AgNCs) were modified with 4-mercaptobenzene boronic acid (4-MPBA) as a Raman reporter for the quantitative detection of DA. The nanogaps between AgNCs and the AgNF led to the generation of an abundance of hot spots for the SERS signal and thus effectively improved the sensitivity of DA detection. Measurements of DA concentrations in clinical body fluids such as human serum and urine samples are also demonstrated, showing excellent performance for DA detection in a complex environment. Our results demonstrate the promising potential for the ultrasensitive detection of DA for the potential diagnosis of DA-related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Jin-Feng Guan,Jiao Zou,Yi-Ping Liu,Xin-Yu Jiang,Jin-Gang Yu
Based on a hybrid carbon nanotube composite, a novel electrochemical sensor with high sensitivity and selectivity was designed for the simultaneous determination of dopamine (DA) and uric acid (UA). The hybrid carbon nanotube composite was prepared by ultrasonic assembly of carboxylated multi-walled carbon nanotube (MWCNT-COOH) and hydroxylated single-walled carbon nanotube (SWCNT-OH). And the hybrid (MWCNT-COOH/SWCNT-OH) composite was characterized by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared (FT-IR) spectroscopy. The electrochemical performances of MWCNT-COOH/SWCNT-OH composite modified glassy carbon electrode (MWCNT-COOH/SWCNT-OH/GCE) were analyzed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Under the optimum experimental conditions, the as-prepared sensor showed high sensitivity and selectivity for DA and UA. The calibration curves obtained were linear for the currents versus DA and UA concentrations in the range 2–150 μM, and limits of detection (LODs) were calculated to be 0.37 μM and 0.61 μM (signal-to-noise ratio of 3, S/N = 3), respectively. The recoveries of DA and UA in bovine serum samples at MWCNT-COOH/SWCNT-OH/GCE were in the range 96.18–105.02%, and relative standard deviations ( RSDs ) were 3.34–7.27%. The proposed electrochemical sensor showed good anti-interference ability, excellent reproducibility and stability, as well as high selectivity, which might provide a promising platform for determination of DA and UA.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Qi Kang,Qiao Zhang,Lixin Zang,Mengru Zhao,Xiaolan Chen,Dazhong Shen
Molecularly imprinting polymers (MIPs), as artificial antibodies with high recognition selectivity to template molecules, are widely used in various biosensors . To improve further the selectivity of MIPs-based photoelectrochemical (PEC) biosensors, we report a differential strategy using non-imprinted polymers (NIPs) as the reference. In a proof-to-concept example for the determination of dopamine (DA), MIPs and NIPs membranes were fabricated by electrochemical polymerization of polypyrrole membranes on the surface of graphene quantum dots (GQDs)/TiO 2 nanotubes (NTs). The photocurrent difference between the two PEC cells , MIPs@GQDs/TiO 2 NTs−Pt and NIPs@GQDs/TiO 2 NTs−Pt, was measured as the signal. As the non-specific adsorption of non-template molecules on the outside surface of MIPs and NIPs membranes is similar, the anti-interference ability for the determination of DA is much improved by using differential strategy. In the normal and differential PEC measurement models, 10.0 μM ascorbic acid is equivalent to 3.12 and 0.40 μM DA, respectively. Further, the smaller specific surface area in NIPs membrane was compensated by using a weight factor to correct the residual interference in a modified differential model. By using 10.0 μM ascorbic acid as the balance point, the presence of 10.0 μM H 2 O 2 , glutathione , uric acid or glucose is equivalent only to 0.090, 0.061,0.11 or 0.041 μM of DA, respectively, which are about 3–7% of their interference levels in the normal photocurrent model. The differential PEC method was applied in the determination of DA in serum samples in the linear range of 0.05–12.5 μM, with the detection limit of 0.018 μM.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 10
·3605-3611
·
IF 3.978
Li-Ping Jia,Zhe Feng,Ruo-Nan Zhao,Rong-Na Ma,Wei Zhang,Lei Shang,Wen-Li Jia,Huai-Sheng Wang
A sensitive and enzyme-free electrochemical aptasensor was constructed for the sensing of 8-hydroxy-2′-deoxyguanosine (8-OH-dG). In the process of constructing the aptasensor, triple signal amplification strategies were introduced to enhance the sensitivity. First, every aptamer/pDNA complex immobilized on magnetic beads could release three kinds of pDNAs when 8-OH-dG was introduced, which caused three-fold magnification of the target. Second, the released three kinds of pDNAs initiated catalyzed hairpin assembly between two hairpin DNAs (HP1 and HP2) on a gold electrode. Meanwhile, the three kinds of pDNAs were released again by a strand displacement reaction to obtain the next catalyzed hairpin assembly. Third, the emerging toehold of HP2 further induced a hybridization chain reaction (HCR) between two hairpin DNAs (HP3 and HP4), forming a long double-stranded DNA concatemer on the surface of the electrode. Finally, [Ru(NH3)6]3+, an electroactive cation, was adsorbed onto the long dsDNA concatemer by electrostatic interactions and consequently, an electrochemical signal was generated. Under this triple signal amplification, a low detection limit down to 24.34 fM has been obtained for 8-OH-dG determination, which is superior to those of most previously reported methods.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Zhang Qiao,Zhang Fengxia,Yu Lei,Kang Qi,Chen Yuqin,Shen Dazhong
A differential photoelectrochemical (PEC) method for glucose determination is reported using a nanocomposite with double mimic enzymes of glucose oxidase (GOx) and peroxidase. The nanocomposite was prepared by soaking zeolite imidazole framework-67 (ZIF-67) in 0.1 M NaOH solution at room temperature for 30 min, abbreviated as Co x O y H z @ZIF-67. The Michaelis–Menten constant of Co x O y H z @ZIF-67 to H 2 O 2 and glucose is 121 μM and 3.95 mM, respectively. Using the photoelectrode of Co x O y H z @ZIF-67/TiO 2 nanotubes (NTs), glucose was oxidized firstly by dissolved oxygen to generate H 2 O 2 under the catalysis of Co x O y H z film as the mimics of GOx. The product of H 2 O 2 enhanced the photocurrent of TiO 2 NTs under the catalysis of ZIF-67 as the mimics of peroxidase. The molecular sieve effect of ZIF-67 frameworks reduces the interferences from molecules with size larger than the apertures in ZIF-67. Under the excitation of a 150 W xenon lamp with full spectrum, the photocurrent was measured in a two-electrode system without external additional potential. By using the photocurrent difference between two photocells, i.e Co x O y H z @ZIF-67/TiO 2 NTs and Pt electrode, ZIF-67/TiO 2 NTs and Pt electrode, as the signal, the selectivity for glucose determination is improved further. The differential PEC method was applied to the determination of glucose with a linear range 0.1 μM~1 mM and a detection limit of 0.03 μM.
Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Haiyan Zhang,Sen Liu
Developing efficient approachs to synthesize nitrogen-doped graphene materials is significantly important. Herein, it is reported that nitrogen-doped reduced graphene oxide (N-rGO) had been successfully prepared by pyrolysis of poly( p -phenylenediamine)-rGO (P p PD-rGO) hybrids. The combined characterizations of Infrared (IR) spectra, Raman spectra, scanning electron microscopy (SEM) were used to characterize the structure of N-rGO. Most importantly, such N-rGO shows good sensing performances for simultaneous detection of ascorbic acid (AA), dopamine (DA), and uric acid (UA). The linear ranges of the sensors for AA, DA, and UA are 0.1 mM–4 mM, 1 μM–60 μM and 1 μM–30 μM. The detection limit for detection of AA, DA and UA are 9.6 μM, 0.1 μM and 0.2 μM, respectively. This work provides a highly effective approach for preparation of graphene-based materials for electrochemical sensing applications.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Di Zhu,Zhenyuan Bai,Huiyuan Ma,Lichao Tan,Haijun Pang,Xinming Wang
Developing a high-efficient and low-cost nanocomposite, featured by abundant active sites with excellent transfer ability for electrons and high conductivity, for the simultaneous detection of biomolecules belonging to different systems is critically important and challenged. Herein, a nanocomposite composed of ferroferric oxide nanoparticles (Fe 3 O 4 ) functionalized polyoxometalates (H 6 PMo 9 V 3 O 40 ·nH 2 O) was synthesized, using one-step hydrothermal doped method. It exhibited high electrocatalytic activity for oxidation of β-nicotinamide adenine dinucleotide (NADH) and l -tryptophan ( l -trp) with well separated signals (Δ =180 mV) between them coming from the combination of PMoV and Fe 3 O 4 . The PMoV@Fe 3 O 4 /GCE-based sensor exhibited outstanding sensing capability for NADH and l -trp simultaneously with low detection limit of 53 nM and 17 nM, respectively, and excellent selectivity over various possible interfering species, which originated from the synergistic effect of PMoV and Fe 3 O 4 . Moreover, the practical application potential of the PMoV@Fe 3 O 4 sensor was successfully demonstrated by determining NADH and l -trp in real samples.
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Yu Cai,Bo Liang,Shidie Chen,Qin Zhu,Tingting Tu,Ke Wu,Qingpeng Cao,Lu Fang,Xiao Liang,Xuesong Ye
The single-step modification of the nanostructured polyaniline (PANI)/glucose oxidase (GOD) enzyme on double-sided, screen-printed, flexible electrodes doped with Prussian blue (PB), has been achieved and successfully applied in continuous glucose monitoring in vivo, and its biocompatibility has been evaluated systematically. The proposed fabrication procedure is simple, low cost, and suitable for large-scale production. PB doped with carbon ink catalyzes the reduction of hydrogen peroxide (H 2 O 2 ) in low-voltage conditions, which could help eliminate interferences. And the PANI/GOD nanostructure makes the GOD enzyme more stable for long-term, in vivo monitoring. More importantly, a polyurethane (PU) layer is deposited on the electrode's surface as a diffusion limiting membrane that enhanced the linear range and biocompatibility. In tests in vitro, the proposed biosensor achieved a linear range of 0–12 mM and a good sensitivity of 16.66 μA·mM −1 ·cm −2 (correlation coefficient R 2 = 0.9962) with an excellent specificity to glucose. The biosensor exhibits long-term stability, with a maximum lifespan of 14 days when stored in phosphate buffer solution at 4 °C, and achieves a sensitivity of 120%. The biocompatibilities of the electrode materials have also been systematically evaluated in cytotoxicity and cell adhesion tests to ensure the safety of implantation. In experiments in vivo, the biosensor can successfully monitor the glucose level fluctuation of rats after 24 h following implantation. Overall, the biosensor fabricated with the double-side, screen-printing process, satisfies the glucose monitoring range in vivo and eliminates various types of interference, thus establishing a new, large-scale production procedure for flexible in vivo biosensors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 31
·期 32
·325502
·
IF 3.551
Nianfeng Shi,Shupei Sun,Bo Zhang,Qian Du,Yanxin Liao,Xiaoming Liao,Guangfu Yin,Zhongbing Huang,Ximing Pu,Xianchun Chen
Co(OH)2 nanosheets/Cu(OH)2 nanorods composite electrodes for non-enzymatic glucose detection were fabricated by electrodepositing Co(OH)2 nanosheets on Cu(OH)2 nanorods substrate grown directly on the copper sheet via a simple one-step reaction. The Co(OH)2 nanosheets/Cu(OH)2 nanorods composite electrode was characterized by scanning electron microscopy, energy dispersive x-ray spectroscopy, x-ray diffraction and x-ray photoelectron spectroscopy. The glucose sensing performance of the composite electrode was investigated by cyclic voltammetry and chronoamperometry. The composite electrode shows high sensitivity of 2366 µA mM−1 cm−2 up to 2 mM with a lower detection limit of 0.17 mM (S/N = 3). The composite electrode is highly selective to glucose in the presence of various substances that always co-exist with glucose in real blood samples. The response of the composite towards human blood serum was in good agreement with that of commercially available glucose sensors, suggesting that a promising electrode material for highly sensitive and selective non enzymatic detection of glucose can be envisioned.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Shasha Pang,Xianwen Kan
In this work, a facile, one-pot, and green synthetic approach was proposed to prepare a novel nanocomposite of nitrogen doped graphene-thionine-gold nanoparticles (NG-Thi-AuNPs), which was then modified on electrode to fabricate an electrochemical sensor for diethylstilbestrol (DES) accurate and reliable detection based on ratiometric strategy. The synthesized NG-Thi-AuNPswas characterized by transmission electron microscope, scanning electron microscope, Fourier transform infrared spectrum, and X-ray diffraction. Due to the merits of good conductivity and high electroactive surface of NG and AuNPs, as well as the synergistic interaction toward DES, the nanocomposite showed high sensitivity for DES detection. Moreover, the modified Thi exhibited a constant current response regardless how the DES concentration changed, which made Thi to be a good inner reference to improve the accuracy and reliability of the sensor. Thus, a ratiometric signal (Δ i DES /Δ i Thi ) was measured and calculated for DES detection with a wide linear range and a low limit of detection of 5.0 × 10 −8 - 8.0 × 10 −5 mol/L and 9.6 × 10 −9 mol/L, respectively. Due to the excellent conductivity and high electrocatalytical activity of the nanocomposite, the sensor was employed for the sensitive detection of H 2 O 2 with the linear range of 5.0 × 10 −7 - 1.0 × 10 −2 mol/L. With the good anti-interference, reproducibility, and stability, the prepared sensor was successfully used for real samples determination.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）