Molecular Catalysis
·
2020
·
IF 3.687
Huan Fu,Luna Ruan,Jianhua Liao,An Pei,Jun Liu,Li Zeng,Kai Yang,Lihua Zhu,Bing Hui Chen
In this report, the Ni/C sample was successfully prepared at room temperature (RT) by depositing nickel related species on carbon black by a chemical liquid reduction method with hydrazine hydrate as reducing agent, Pt/C was synthesized via a wetness impregnation method. And Pt was supported on Ni/C by galvanic replacement reaction to form PtNi/C bimetallic nanocatalyst. By comparing the catalytic performance of different catalysts for 1-nitronaphthalene hydrogenation, it could be found that the PtNi/C bimetallic nanocatalyst showed higher catalytic activity than monometallic nanocatalysts (Pt/C and Ni/C), exhibiting 100 % conversion of 1-nitronaphthalene, 100 % selectivity to 1-naphthylamine and turnover frequency of 194.9 h −1 under mild reaction conditions (40 min, 30 °C, 3.0 MPa H 2 ). The as-prepared catalysts were characterized by XRD, XPS, HAADF-STEM, STEM-EDX mapping and line-scanning, TG, HRTEM and H 2 -TPD, which proved that PtNi/C showed most outstanding catalytic behaviors in 1-nitronaphthalene selective hydrogenation due to high dispersion of platinum, the nanostructure of Pt-on-Ni-on-Ni(OH) 2 and thus synergism effect among Pt, Ni and Ni(OH) 2 .
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2018
·Vol. 135
·期 6
·3005-3013
·
IF 2.209
Luo Liqiong,Jin Bo,Peng Rufang,Shang Yu,Xiao Lipengcheng,Chu Shijin
A new nitrogen-rich energetic salt of bis-1-methylimidazole 1 H ,1′ H -5,5′-bistetrazole-1,1′-diolate salt, (1- M ) 2 BTO, was synthesized and characterized (FT-IR, 1 H NMR, 13 C NMR, elemental analysis, and X-ray single-crystal diffraction). Results indicated that (1- M ) 2 BTO crystallizes in the triclinic space group P -1. The thermal decomposition behavior of (1- M ) 2 BTO was determined by differential scanning calorimetry (DSC) and thermogravimetric tandem infrared spectroscopy. The decomposition peak temperature of (1- M ) 2 BTO was 530 K, which suggested that the salt is strong heat resistance. The apparent activation energies were 130.56 kJ mol −1 (Kissinger’s method) and 132.50 kJ mol −1 (Ozawa’s method), respectively. The enthalpy of formation for the salt was calculated as 917.3 kJ mol −1 . The detonation velocity and detonation pressure of (1- M ) 2 BTO were 7448 m s −1 and 20.7 GPa, respectively, using the Kamlet-Jacobs equation. Furthermore, the sensitivity test results showed that its impact sensitivity is greater than 50 J and friction sensitivity is 180 N, indicating that it has a lower sensitivity.
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eLife
·
2024
·
IF 6.4
Shu-Ting Zhang,Shi-Kai Deng,Tao Li,Megan E Maloney,De-Feng Li,Jim C Spain,Ning-Yi Zhou
1-Naphthylamine (1NA), which is harmful to human and aquatic animals, has been used widely in the manufacturing of dyes, pesticides, and rubber antioxidants. Nevertheless, little is known about its environmental behavior and no bacteria have been reported to use it as the growth substrate. Herein, we describe a pathway for 1NA degradation in the isolate Pseudomonas sp. strain JS3066, determine the structure and mechanism of the enzyme NpaA1 that catalyzes the initial reaction, and reveal how the pathway evolved. From genetic and enzymatic analysis, a five gene-cluster encoding a dioxygenase system was determined to be responsible for the initial steps in 1NA degradation through glutamylation of 1NA. The γ-glutamylated 1NA was subsequently oxidized to 1,2-dihydroxynaphthalene which was further degraded by the well-established pathway of naphthalene degradation via catechol. A glutamine synthetase-like (GS-like) enzyme (NpaA1) initiates 1NA glutamylation, and this enzyme exhibits a broad substrate selectivity toward a variety of anilines and naphthylamine derivatives. Structural analysis revealed that the aromatic residues in the 1NA entry tunnel and the V201 site in the large substrate-binding pocket significantly influence NpaA1’s substrate preferences. The findings enhance understanding of degrading polycyclic aromatic amines, and will also enable the application of bioremediation at naphthylamine contaminated sites.
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PubMed
高影响因子
REPRODUCTIVE TOXICOLOGY
·
2025
·
IF 2.8
Huage Liu
Thirdhand smoke (THS), a residual cigarette smoke still remaining in indoor environment long after active smoking has ceased, has aroused more and more people’s attention on health. However, the impact of THS exposure on the male reproductive system, particularly in in-vivo study, remain unclear. Thus, this study aims to investigate the adverse effects of 1-(N-methyl-N-nitrosamino)-1-(3-pyridinyl)-4-butanal (NNA), a special component in THS, on testis. Herein, adult ICR mice were orally administered diluted dimethylsulfoxide (control), 0.075 μg/kg and 0.15 μg/kg NNA for 10 weeks. The findings revealed that NNA exposure, especially in 0.15 μg/kg dosage, significantly reduced the epithelium height and tubular area within the testis. Transcriptomic analysis indicated that NNA exposure interfered with the expression of genes associated with oxygen transport and oxygen binding processes, such as Hbb-bs , the downregulation of which also led to oxidative damage. Additionally, NNA exposure induced oxidative damage in testis by decreased levels of SOD and GSH enzymes, along with an increase in MDA level. In summary, our results demonstrate that NNA exposure causes impaired testicular structure and oxidative damage, indicating its testicular toxicity. This finding not only implies the detrimental effect of NNA on male reproduction, but also provides a fundamental basis for further research on the impact of THS exposure on male reproductive health.
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PubMed
高影响因子
POLYHEDRON
·
2024
·
IF 2.4
An-Na Chen,Yan-Ling Deng,Chun-Yang Pan
Two imidazole-based ligand-nickel borates, [Ni(1-ipIM) 3 ·B 6 O 7 (OH) 6 ]·[H 3 BO 3 ]·2·.5H 2 O ( 1 , 1-ipIM = 1-isopropylimidazole) and [Ni(1-pIM) 6 ]·[B 5 O 6 (OH) 4 ]·2H 2 O ( 2 , 1-pIM = 1-propylimidazole) have been synthesized and structurally characterized by single-crystal X-ray diffraction, PXRD, FTIR. The oxoboron cluster structures of 1 and 2 were modulated through changing the carbon-branched structure on the N -site of the imidazole-based ligand using metallic nickel as the central atom. It is noteworthy that the structure of 1 is a hybridized skeleton formed by N -Ni-O-B bonds. The electrocatalytic oxygen reduction reaction (ORR) activity of these borate-based catalysts was investigated. The half-wave potential ( E 1/2 ) values of 1 -based and 2 -based were about 0.825 and 0.80 V, respectively.
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FOOD AND CHEMICAL TOXICOLOGY
·
2019
·
IF 3.775
Congyong Sun,Wenjing Li,Yingkun Liu,Wenwen Deng,Michael Adu-Frimpong,Huiyun Zhang,Qilong Wang,Jiangnan Yu,Ximing Xu
1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside (MPG) is a phenolic glycoside that exists in Moringa oleifera seeds with various health benefits, whereas its hepatoprotective effect is lacking clarification. Herein, MPG was isolated from Moringa oleifera seeds, and its hepatoprotection against CCl 4 -induced hepatotoxicity in L02 cells and ICR mice was investigated. Toxicity studies showed that MPG did not induce significant changes in organ coefficients and histological analysis, as well as exhibited no cytotoxicity. In vitro studies indicated that MPG substantially increased cell viability and intracellular SOD activities, and significantly inhibited LDH leakage in CCl 4 -treated cells. In vivo studies demonstrated that MPG significantly alleviated CCl 4 -induced hepatotoxicity in mice, as indicated by diagnostic indicators of hepatic injury, as well as the histopathological analysis. Moreover, MPG reduced the lipid peroxidation levels and regulated the inflammatory cytokines. Notably, MPG substantially suppressed the significant elevation of ROS production in hepatocytes of mice intoxicated with CCl 4 . Moreover, TUNEL assay demonstrated that MPG obviously inhibited hepatic apoptosis induced by CCl 4 . Altogether, these results suggested that MPG has excellent liver-protecting effects against hepatocytotoxicity induced by CCl 4 in mice and L02 cells, which can be further developed as a valuable functional food additive or drug for the treatment of hepatic injury.
研究领域:
药理学
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毒理学
·
肝脏病学
·
营养保健品
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PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 3.8
Xue-Chun Li,Hong-Jie Xie,Zheng-Xiong Zhou,Bin Sun,Jun-Jie Lin,Xiao-Dan Li,Ren Wang
1-aminocyclopropane-1-carboxylic acid (ACC), a crucial intermediate in the ethylene biosynthetic pathway, plays a decisive role in regulating plant developmental processes and stress responses. In heterologous biosynthesis systems, ACC biosynthesis is inhibited due to its antibacterial activity. Enhancing ACC export confers advantages to ACC-producing biotechnological platforms and microbial cell factories. While bacterial transporters for canonical amino acids in Corynebacterium glutamicum and Escherichia coli have been extensively studied, their roles in ACC transport remain unexplored. To identify the exporters mediating ACC transport in E. coli , relevant experiments were performed in this study. The results showed that the Leucine-exporter LeuE acts as a high-efficiency ACC exporter in E. coli . Subsequently, structural modeling coupled with site-directed mutagenesis revealed that L160 is a critical residue in determining the ACC transport specificity of LeuE. The L160Y mutation enhanced E. coli tolerance to ACC, elevating the threshold from 50 mM to 100 mM. Structural modeling predicted that the L160Y mutation-induced compaction reduced the transporter channel diameter by 28% (from 9.551 Å to 6.876 Å), a change that may enhance the binding affinity of ACC. Notably, a significant reduction in intracellular ACC levels was observed in both the LeuE-overexpressing and LeuE (L160Y)-overexpressing strains. These findings enable large-scale biosynthesis of ACC and its derivatives via engineered microbial systems, thereby facilitating cost-effective development of ACC-based pharmaceuticals and agrochemicals.
研究领域:
微生物学
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分子生物学
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生物技术
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代谢工程
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转运生理学
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高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Hong Li,Yuanyuan Feng,Jingde Li,Yanqin Yang
Efficient CO2 capture, demonstrating significant potential in reducing excessive carbon emissions and advancing carbon neutrality, is crucial for advancing carbon capture, utilization, and storage (CCUS) technology. Membrane technology is environmentally friendly and energy-saving for CO2 capture. Mixed matrix membranes (MMMs) consisting of polymers of intrinsic microporosity (PIMs)-grafted composite fillers and PIM matrices are promising for gas separation. However, conventional two-phase MMMs often face a fundamental challenge: the trade-off between permeability and selectivity, coupled with nonselective interfacial defects arising from poor compatibility between the polymer matrix and fillers. Moreover, the synthesis of composite fillers is usually complex and highly dependent on the reasonable matching of fillers and grafted polymers, restraining the wide application of this material system. To fundamentally address this issue of interfacial compatibility, we focused on designing a distinct third phase at the interface. Herein, we report a concept of polymer–filler–polymer ternary-phase MMMs (TMMMs) for efficient CO2 separation. The key point is that the membrane system consists of a PIM matrix, filler, and a functionalized PIM-type third component. As the functionalized PIMs selected can form strong interactions with both fillers and PIM matrices, they can work as a molecular bridge between two phases, diminishing the generation of nonselective interfacial defects. Meanwhile, the functionalized PIMs’ incorporation can increase the quantity of CO2 adsorption sites as well as facilitate the formation of a porous network structure between filler and matrix phases, making the performance improvement of the resultant membranes foreseeable. Particularly, the representative ternary membrane made from 3D-COF (filler, 5 wt %), PIM-1 (matrix), and carboxylated PIM-1 (cPIM-1, third component, 1 wt %) exhibits significantly improved CO2 permeability of 9560 Barrer, CO2/N2 selectivity of 22.7, and antiaging performance in comparison to the pure PIM-1 membrane and PIM-1/3D-COF binary membrane. We believe that this concept proposed here offers an alternative way to produce defect-free and high-performing PIM-based MMMs for CO2 capture and other separations.
研究领域:
材料科学
·
膜技术
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碳捕集与封存
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高分子化学
·
气体分离
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高影响因子
MicrobiologyOpen
·
2018
·Vol. 8
·期 5
·e00721
·
IF 2.682
Can Chen,Keqi Chen,Tao Su,Bing Zhang,Guizhi Li,Junfeng Pan,Meiru Si
Graphical The ino-1 deletion mutant showed obviously higher ROS levels than the WT(pXMJ19) and the complement Δino-1(pXMJ19-ino-1) strains under oxidative stress conditions. And clearer protein carbonyl groups were detected in the Δino-1(pXMJ19) strains after exposure to ROS-generating agents compared to that in the WT(pXMJ19) strains. Reactive oxygen species (ROS) generated in aerobic metabolism and oxidative stress lead to macromolecules damage, such as to proteins, lipids, and DNA, which can be eliminated by the redox buffer mycothiol (AcCys-GlcN-Ins, MSH). Myo -inositol-phosphate synthase (Ino-1) catalyzes the first committed step in the synthesis of MSH, thus playing a critical role in the growth of the organism. Although Ino-1s have been systematically studied in eukaryotes, their physiological and biochemical functions remain largely unknown in bacteria. In this study, we report that Ino-1 plays an important role in oxidative stress resistance in the gram-positive Actinobacteria Corynebacterium glutamicum . Deletion of the ino-1 gene resulted in a decrease in cell viability, an increase in ROS production, and the aggravation of protein carbonylation levels under various stress conditions. The physiological roles of Ino-1 in the resistance to oxidative stresses were corroborated by the absence of MSH in the Δ ino-1 mutant. In addition, we found that the homologous expression of Ino-1 in C. glutamicum yielded a functionally active protein, while when expressed in Escherichia coli BL21(DE3), it lacked measurable activity. An examination of the molecular mass (Mr) suggested that Ino-1 expressed in E. coli BL21(DE3) was not folded in a catalytically competent conformation. Together, the results unequivocally showed that Ino-1 was important for the mediation of oxidative resistance by C. glutamicum .
研究领域:
微生物学
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分子生物学
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生物化学
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细菌生理学
·
氧化应激
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高影响因子
INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Shuai Chen,Ding-Chong Han,Le Ye,Wei-Xiong Zhang
Hybrid ferroelastic crystals have emerged as a hot research topic in recent years owing to their prospective applications in piezoelectric sensors, mechanical switches, and optoelectronic devices. Nevertheless, most of the documented materials exhibit one-step or two-step ferroelastic phase transition(s), and those with multistep ferroelastic transitions are extremely scarce. We present a new hexagonal molecular perovskite based on a fluoro-substituted flexible cyclic ammonium cation, (1-fluoromethyl-1-methylpyrrolidine)[CdCl3] (1), undergoing unusual three-step ferroelastic phase transitions from hexagonal paraelastic phase to orthorhombic, monoclinic, and triclinic ferroelastic phases at 388, 376, and 311 K, respectively, with Aizu notation of 6/mmmFmmm, mmmF2/m, and 2/mF-1, featuring spontaneous strain of 0.002, 0.023, and 0.110, respectively. Furthermore, variable-temperature single-crystal diffraction reveals that the phase-transition mechanism in 1 principally originates from intriguing dynamic change of organic cations and synchronous displacement of inorganic chains. This scarce instance of multistep hybrid ferroelastic provides important clues for finding advanced ferroelastic materials.
研究领域:
材料科学
·
晶体学
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FREE RADICAL BIOLOGY AND MEDICINE
·
2026
·
IF 8
Zhengyu Wu,Huimou Chen,Yu Zhou,Sidong Zhang,Zhihua Li,Yadong Song,Jing Hu
Ewing sarcoma is an aggressive pediatric bone cancer with poor prognosis, highlighting the urgent need for novel therapeutics. This study demonstrates the potent anti-tumor activity of shogaol, a bioactive compound from ginger, against Ewing sarcoma, using both in vitro and in vivo model systems. In vitro, shogaol significantly inhibited cell proliferation and induced both apoptosis and ferroptosis in Ewing sarcoma cells, as evidenced by increased lipid reactive oxygen species, Malondialdehyde, and Fe 2+ levels, as well as altered expression of BAX, BCL2, GPX4, and SLC3A2. In vivo , shogaol significantly inhibited tumor cell growth in a subcutaneous xenograft mouse model using SKNMC cells. Using transcriptome sequencing and proteomics, we found that shogaol targeted the PLK1/NPM1 axis: it downregulated polo-like kinase 1 (PLK1), leading to reduced phosphorylation of NPM1 at Thr199. This facilitated the increased interaction between the E3 ligase TRIM28 and NPM1, leading to its ubiquitination and subsequent degradation, which in turn inhibited the MAPK signaling pathway. Key findings were validated by CETSA, isothermal dose-response fingerprinting, co-immunoprecipitation, and ubiquitination assays. These results demonstrate that shogaol exerts dual cytotoxic effects through the PLK1/NPM1 axis, presenting a potential natural therapeutic approach for Ewing sarcoma.
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高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Songlin Li,Chen Wang,Zhaohui Lei,Shanfu Sun,Jie Gao,Pengfei Cheng,Hai Wang
A deep understanding of the relationship between the properties and structure of transition metal oxide semiconductor gas sensors is essential for optimizing sensor design. Comparing and analyzing the sensing properties of nanomaterials with different morphologies and crystal facets by combining the experiment analysis and the density functional theory (DFT) calculations could identify and understand the corresponding mechanisms at the atomic or ionic level. Here, grown Co 3 O 4 nanosheet (Co 3 O 4 -NS) with exposed (1 1 1) facets, and Co 3 O 4 nanorods (Co 3 O 4 -NR) with exposed (1 1 0) facets on alumina ceramic tubes were prepared and studied in sensing ethanol. The adsorption characteristics of ethanol on the sensor surface were analyzed by DFT calculation. The results demonstrated that the sensing performance of Co 3 O 4 -NR was superior to Co 3 O 4 -NS. The adsorption of hydroxyl groups in ethanol was better than the methyl groups on the surface. The oxygen and hydrogen atoms from the hydroxyl groups in ethanol had the lowest adsorption energies (E ads = −3.44 eV) when they interact with the Co atom and the indirectly adjacent low-coordinate oxygen atoms on the (1 1 0) surface, respectively. The synergistic adsorption of target gases by exposed surface atoms induced superior sensing properties of the material.
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