ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jing Tang,Guangrong Zou,Chen Chen,Jing Ren,Fang Wang,Zilin Chen
The identification of formylpyrimidines in DNA is crucial for a better understanding of epigenetics. Although many techniques have been explored to detect their content, more accurate methods of formylpyrimidine determination are still required due to the relatively lower sensitivity or lack of selectivity in current methods. Herein, an electrochemical method based on the covalent bonding of the azido derivative of (2-benzimidazolyl) acetonitrile (azi-BIAN) and the aldehyde group of 5-formyluracil (5fU) was proposed for the selective detection of 5fU in the presence of 5-formylcytosine (5fC) and apyrimidinic (AP) sites. Target DNA containing 5fU was first treated with azi-BIAN and then incubated with DBCO-PEG4-Biotin to introduce a biotin group by copper-free click chemistry. Next, the sulfhydryl group was attached to the 5′ end of above DNA through T4 polynucleotide kinase-catalyzed reaction. Subsequently, the labeled DNA was assembled onto the AuNPs-modified glassy carbon electrode (AuNPs/GCE) through Au–S bonds, and the streptavidin–horseradish peroxidase conjugate (SA-HRP) was further immobilized onto the surface of the above electrode by specific recognition between biotin and streptavidin. Finally, HRP catalyzed hydroquinone oxidation to benzoquinone to enhance the current signal, which was related to the amount of 5fU in nucleic acids. This method demonstrated a good linear relationship with 5fU concentrations ranging from 0.1 to 10 nM. Moreover, the level of 5fU in γ-irradiated nucleic acids was also successfully detected, indicating that the combination of molecule-depended chemical recognition and electrochemical sensing is a promising method for the selective and sensitive detection of 5fU.
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VETERINARY PARASITOLOGY
·
2022
·
IF 2.821
Ying Zhang,Long Cheng,Hongyu Qiu,Tiancong Sun,Ruipeng Deng,Haiyan Gong,Rongsheng Mi,Yan Huang,Chunren Wang,Zhaoguo Chen
Bromodomain (BRD) is a highly conserved structural module domain, found in various proteins, including chromatin-related proteins, nucleus acetyltransferases, and transcription-associated proteins. Toxoplasma gondii , a zoonotic protozoan, encodes at least 12 predicted BRD-containing proteins (BDPs). Here, we investigated the subcellular location and regulatory role of a hypothetical protein BDP that we named TgBDP5. The BRD of TgBDP5 did not contain the conserved Asn and Tyr residues required for acetyl-lysine recognition. TgBDP5 localized in the nucleus of the parasite and remained unchanged during parasite replication. Conditional ablation of TgBDP5 through an auxin-inducible degron-based knockdown strategy caused a growth defect in parasite replication. Depletion of TgBDP5 led to changes in the expression level of 179 genes, suggesting it as an important target for drugs acting against T. gondii .
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CATALYSIS TODAY
·
2022
·
IF 6.562
Luxin Ren,Wangchen Huo,Guiying Li,Wonyong Choi,Taicheng An
Photocatalytic oxidation and micropollutants have both attracted extensive attention in the field of wastewater treatment . However, invalidation mechanism of photocatalytic system and target transformation are still a challenge. Here, we used TiO 2 supported on nickel foam (TiO 2 /NF) and 5-fluorouracil (5-FU) as model photocatalyst and pharmaceutical micropollutant to investigate photocatalytic transformation pathways of 5-FU and inhibition mechanisms of its degradation efficiency reduction. Incredibly, removal efficiency of 5-FU dramatically decreased from 80% to 20% after 120 h continuous operation, owning to the competition for hydroxyl radical ( • OH) between trans-intermediates and 5-FU, accumulated in the solution. Using HR-MS, the produced intermediates during photocatalytic degradation of 5-FU were revealed to be that mono/polyhydroxylated compounds, short-chain oxides and carboxylation ones were main trans-intermediates. Combining ATR-FTIR, SEM-EDS and validation experiments for factors, it was found that the inhibition of 5-FU degradation should be contributed to the competition for • OH in the bulk or preferential consumption of • OH in TiO 2 diffusion layer, by the aforementioned intermediates. The present study provides insights into photocatalytic oxidation of 5-FU and basic information about mechanisms of inhibition on target contaminant removal.
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FUEL
·
2025
·
IF 7.5
Qicheng Li,Xihu Zhao,Xiaobo Song,Juan Dong,Zhitong Zhao,Pan Hao,Qi Shi,Xuekuan Li
Benzene alkylation with syngas is attractive for alkylbenzene production, which use metals/metal oxides combining H-ZSM-5 zeolites as the catalyst. Herein, the H-ZSM-5 zeolites with three different morphologies—spindle-, flake-, and nano-sized—were synthesized. Subsequently, crystal structural, textural and acidic properties of three H-ZSM-5 zeolites and their corresponding bifunctional catalysts were characterized and the catalytic reaction activity and stability of the benzene alkylation with syngas were investigated. The bifunctional catalyst derived from nano-sized zeolites demonstrates an initial benzene conversion rate of 46.7% and reduces by 71.78% after 51 h of continuous operation, owing to abundant acid sites, large surface area and mesopores diameters, and balanced acidity to promote reactant and product diffusion. The spindle-shaped zeolite catalyst delivers the highest initial benzene conversion rate (53.77%) due to abundant acid sites. However, the benzene conversion rate drops to 12.12% of the initial value after 39 h due to limited micropore volume and relatively small mesopore diameters of the catalyst. The flake-shaped zeolite catalyst exhibits the lowest initial conversion rate and the shortest lifetime (30 h) owing to the fewest accessible acid sites and the peculiar pore structure. Three cases indicate that increasing mesopore volume is conducive to improve ratio of Brønsted to Lewis acid sites (B/L), resulting in enhancement of the catalytic activity. Meanwhile, the huge micropore volume and increased mesopore diameter are positive to alleviate pore blockage and coverage of active sites due to high mass-transfer efficiency within the zeolitic channels. These textural advantages collectively contribute to a delayed catalyst deactivation process and sustained catalytic activity. This work highlights the pivotal role of the H-ZSM-5 morphology in the benzene alkylation process with syngas and facilitates the efficient development of production process for non-oil alkylbenzene.
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Current Research in Microbial Sciences
·
2025
·
IF 5.8
Zhi Wu,Yina Shao,Shanshan Zhang,Chenghua Li
Vibrio splendidus AJ01 is the primary pathogen of Apostichopus japonicus , resulting in huge economic losses. In the present study, we found that 5-iodoindole exhibits a remarkable protective effect on A. japonicus , the survival rate of which can be improved to 93.75%. To explore the impact of 5-iodoindole on V. splendidus AJ01, bacterial growth and virulence factors were determined. Results showed that 5-iodoindole significantly decreased swimming motility, hemolytic activity and protease activity but had minimal effect on the overall growth of the bacterium. Furthermore, a differentially expressed protein was downregulated and identified as elongation factor thermo unstable protein (EF-Tu) via SDS-PAGE combined with LC-MS/MS. Moreover, 5-iodoindole had impact on a large number of genes at RNA level, such as genes related to two-component system, ABC transporters, flagella, and chemotaxis. In addition, 20 µM 5-iodoindole could upregulated the mRNA level of immune related genes in A. japonicus , including IL-17, VEGF and MMP1. Our results indicated 5-iodoindole protect A. japonicus through a two-pronged approach: not only inhibiting bacterial virulence but also enhancing the host's immunity. This not only addresses the limitations of traditional indole but also provides a green alternative to antibiotics for protecting A. japonicus from infections caused by V. splendidus AJ01.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Baoxia Liu,Haoshuang Shen,Daosheng Liu,Yuanqiang Hao,Xu Zhu,Qi Shen,Peng Qu,Maotian Xu
Metal-organic coordination polymers have emerged as attractive functional materials due to their tunable structures and unique properties. In this work, by the self-assembly of citrate (Cit) and lanthanide ions, we developed lanthanide coordination polymer nanoparticles (LCPNPs), Cit/Tb, for fluorescence sensing of guanosine-5-monophosphate (GMP). The LCPNP-based Cit/Tb sensor alone displayed weak luminescence, however, the addition of GMP to Cit/Tb led to the formation of a ternary LCPNP, Cit/Tb-GMP, with a significantly enhanced luminescence (15-fold) owing to the intramolecular energy transfer from GMP to Cit/Tb and the hydrophobic environment in the polymer interior. This luminescence turn-on assay exhibited excellent selectivity and high sensitivity for GMP with detection limit of 100 nmol L −1 . Furthermore, the Cit/Tb LCPNP possesses a long luminescence lifetime of up to a millisecond, which is important for detecting GMP in complicated samples of infant formula and ham by time-resolved fluorimetry. We believe that the presented Cit-based nanosensor would inspire the development of various new LCPNP-based fluorescent assays by utilizing Cit or other water-soluble, non-toxic organic ligands for a variety of applications in fluorescence sensing, bio-imaging, drug delivery and so on.
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Scientific Reports
·
2016
·Vol. 6
·期 1
·1-7
·
IF 5.228
Qu Yongshui,Li Li,Wei Quanyuan,Huang Chongpin,Oleskowicz-Popiel Piotr,Xu Jian
Conversion of carbohydrate into 5-hydroxymethylfurfural (5- HMF), a versatile, key renewable platform compound is regarded as an important transformation in biomass-derived carbohydrate chemistry. A variety of ILs, not only acidic but also alkaline ILs, were synthesized and used as catalyst in the production of 5-HMF from disaccharide. Several factors including reaction temperature, IL dosage, solvent and reaction time,were found to influence the yield of 5-HMF from cellobiose. Of the ILs tested, hydroxy-functionalized ionic liquid (IL), 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate ([AEMIM]BF 4 ) showed the highest catalytic activity and selectivity. 5-HMF yield of 68.71% from sucrose was obtained after 6 hrs at 160 °C. At the same condition with cellobiose as substrate, 5-HMF yield was 24.73%. In addition, 5-HMF also exhibited good stablity in this reaction system. Moreover, a kinetic analysis was carried out in both acidic and alkaline IL-catalyzed system, suggesting main side reaction in the conversion of fructose catalyzed by acidic and alkaline IL was polymerization of fructose and 5-HMF degradation, respectively.
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Catalysts
·
2017
·Vol. 7
·期 12
·383
·
IF 3.082
Nana Li,Yadong Bi,Xiaoqiang Xia,Hui Chen,Jianli Hu
The hierarchical HZSM-5 zeolite was prepared successfully by a simple NaOH treatment method. The concentration of NaOH solution was carefully tuned to optimal the zeolite acidity and pore structure. Under NaOH treatment conditions, a large number of mesopores, which interconnected with the retained micropores, were created to facilitate mass transfer performance. There are very good correlations between the decline of the relative zeolite crystallinity and the loss of micropores volume. The Ni nanoclusters were uniformly confined in the mesopores of hierarchical HZSM-5 by the excessive impregnation method. The direct deoxygenation in N2and hydrodeoxygenation in H2of the methyl laurate were compared respectively over the Ni/HZSM-5 catalysts. In the N2atmosphere, the deoxygenation rate of the methyl laurate on the Ni/HZSM-5 catalyst is relatively slow. In the presence of H2, the synergistic effect between the hydrogenation function of the metal and the acid function of the zeolite supports can make the deoxygenation level more obvious. The yield of hydrocarbon products gradually reached the maximum with the appropriate treatment concentration of 1M NaOH, which could be attributed to the improved mass transfer in the hierarchical HZSM-5 supports.
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Nanoscale Advances
·
2020
·Vol. 2
·期 10
·4822-4829
·
IF 0
Yamin Shi,Jiarui Hu,Jian Chen,Yaxin Xu,Wantian Yang,Junnian Chen,Yunbin He
Low-dimensional Pb–Br and Pb–Cl perovskite single crystals have aroused considerable attention due to their broadband white-light emission. But their synthesis involving halogenation of organic amines, dissolution of lead oxide and a slow cooling process is quite complicated. Herein, we report white light emission from mixed-dimensional AVAx(MAPbCl3) perovskite submicron platelets formed by one-step solution processing. It is found that the presence of 5-ammoniumvaleric acid (5-AVA) with a zwitterionic functional group is crucial for modulating the morphology and structural dimensionality of perovskites. Importantly, AVAx(MAPbCl3) perovskites exhibit distinctive structural dimensionality dependent broadband emission, indicating the formation of self-trapped excited states. The AVA2(MAPbCl3) perovskite exhibits white-light emission with a color rendering index (CRI) of 85 and a correlated color temperature (CCT) of 8624 K, yielding “cold” white light. Moreover, the mixed-dimensional perovskite exhibits good stability for more than 30 days. With this report, we aim to provide a facile approach for synthesizing stable low-dimensional perovskite nanostructures for making advanced optoelectronic devices.
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CHINESE JOURNAL OF CHEMICAL PHYSICS
·
2013
·Vol. 26
·期 3
·355-360
·
IF 0.632
Lai Bin,Zhao Yi,Yan Li‐feng
The conversion of cellulose to 5‐hydroxymethylfurfural (HMF) has been investigated by a one‐pot consecutive reaction. At first, cellulose was depolymerised into glucose via a fast degradation of cellulose in molten ZnCl 2 in the presence of hydrochloric acid, and the yield of glucose is 75% in 120 s at reaction temperature of 95 °C. Then, DMSO was used as solvent and different kinds of metal chloride were added as catalysts, and the conversion was carried out continuously at 110–130 °C for 0.5–4 h. The yield of HMF was 53% when CrCl 3 were used as catalyst. The one‐pot two steps conversion was carried out at atmosphere pressure, and it is a simple route to prepare HMF from lignocellulosic feedstock on a large scale.
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Waste and Biomass Valorization
·
2017
·Vol. 9
·期 11
·2181-2190
·
IF 1.337
Han Bin,Zhao Peng,He Rong,Wu Tinghua,Wu Ying
A series of supported B 2 O 3 /ZrO 2 –Al 2 O 3 (BZA) catalysts with different loading B 2 O 3 were prepared and characterized by XRD, FT-IR, XPS, NH 3 -TPD, N 2 adsorption–desorption and FT-IR (pyridine adsorption). These catalysts were applied for direct conversion of glucose to 5-hydroxymethylfurfural (HMF). An optimized HMF yield of 41.2% at a glucose conversion of 90.8% was obtained within 4 h at 150 °C over B 2 O 3 (20 wt%)/ZrO 2 –Al 2 O 3 (BZA-0.20). The best catalytic performance of BZA-0.20 was associated with the highest amount of total acid. The simultaneously bearing Lewis acid and Brønsted acid sites were more efficient for production of HMF from glucose.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2025
·
IF 2.1
Mingya Wang,Hongxia Pan,Yongzheng Liu,Jun Chen,Duanlin Cao,Linxiu Zhao
3(5)-Nitropyrazole is obtained by rearrangement of 1-nitropyrazole in high boiling organic solvents. In this paper, the solubility of 3(5)-nitropyrazole in 12 high boiling solvents (anisole, benzonitrile, mesitylene, o-dichlorobenzene, 1-octanol, benzyl alcohol, 2-ethylhexyl acetate, dimethyl glutarate, cyclohexanone, N,N-dimethylformamide, N-methylformamide, and isophorone) was determined using the static gravimetric method over the temperature range of 283.15–323.15 K. The experimental results revealed that the solubility of 3(5)-nitropyrazole exhibited a positive correlation with temperature. Under ambient conditions at 298.15 K, the solubility decreased in the following order: N,N-dimethylformamide (37.705 × 10–2), N-methylformamide (20.131 × 10–2), cyclohexanone (13.469 × 10–2), isophorone (11.957 × 10–2), dimethyl glutarate (6.667 × 10–2), benzyl alcohol (3.909 × 10–2), benzonitrile (2.915 × 10–2), 1-octanol (1.916 × 10–2), 2-ethylhexyl acetate (1.220 × 10–2), o-dichlorobenzene (0.666 × 10–2), anisole (0.688 × 10–2), and mesitylene (0.246 × 10–2). The solubility of 3(5)-nitropyrazole was fitted using the Apelblat equation, λh equation, NRTL model, and van’t Hoff model. The fitting results were evaluated using average relative deviation and RMSD. The NRTL model exhibited the best overall performance. The use of Hansen solubility parameters for solubility prediction and analysis has certain limitations.
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