INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 55
·期 6
·2509-2516
·
IF 2.773
Shufang Shan,Tianli Zheng,Chaoxiong Zhang,Xuejiao Song,Jiayi Chen,Ying Shi,Jia You,Guo Cheng,Jingyuan Xiong
Summary Antioxidant-rich diets affect telomere length, an integrative marker associated with age-related diseases. Yogurt exerts antioxidative capacity and is speculated to support healthy ageing. However, direct experimental evidence is missing. Here, the effects of yogurt and dairy-fermenting bacteria on D-galactose-induced ageing mice and chemically challenged HepG2 cells were evaluated. Relative telomere length (RTL) in leucocyte and liver were significantly longer in yogurt (1.21 ± 0.07; 1.23 ± 0.11) and Streptococcus thermophilus group (1.18 ± 0.15; 1.13 ± 0.12). When t -BHP-challenged HepG2 cells were treated with digested yogurt and S. thermophilus , the senescence index (13.67 ± 3.30; 19.67 ± 2.87) were lower and RTL (1.25 ± 0.11; 1.18 ± 0.10) were longer than the model. Antioxidative effects were observed for yogurt and S. thermophilus metabolites, whereas milk and Lactobacillus rhamnosus metabolites showed minimal influence on RTL and oxidative stress. In conclusion, this study showed that yogurt and S. thermophilus metabolites ameliorated telomere attrition in ageing mice and t -BHP-challenged HepG2 cells, possibly by reducing oxidative stress.
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Journal of Zhejiang University-SCIENCE B
·
2019
·
IF 1.879
Su-fang Gu,Li-ying Wang,Ying-jie Tian,Zhu-xian Zhou,Jian-bin Tang,Xiang-rui Liu,Hai-ping Jiang,You-qing Shen
Hesperetin, an abundant bioactive component of citrus fruits, is poorly water-soluble, resulting in low oral bioavailability. We developed new formulations to improve the water solubility, antioxidant activity, and oral absorption of hesperetin. Two nano-based formulations were developed, namely hesperetin-TPGS ( D -α-tocopheryl polyethylene glycol 1000 succinate) micelles and hesperetin-phosphatidylcholine (PC) complexes. These two formulations were prepared by a simple technique called solvent dispersion, using US Food and Drug Administration (FDA)-approved excipients for drugs. Differential scanning calorimetry (DSC) and dynamic light scattering (DLS) were used to characterize the formulations’ physical properties. Cytotoxicity analysis, cellular antioxidant activity assay, and a pharmacokinetic study were performed to evaluate the biological properties of these two formulations. The final weight ratios of both hesperetin to TPGS and hesperetin to PC were 1:12 based on their water solubility, which increased to 21.5-and 20.7-fold, respectively. The hesperetin-TPGS micelles had a small particle size of 26.19 nm, whereas the hesperetin-PC complexes exhibited a larger particle size of 219.15 nm. In addition, the cellular antioxidant activity assay indicated that both hesperetin-TPGS micelles and hesperetin-PC complexes increased the antioxidant activity of hesperetin to 4.2-and 3.9-fold, respectively. Importantly, the in vivo oral absorption study on rats indicated that the micelles and complexes significantly increased the peak plasma concentration ( C max ) from 2.64 μg/mL to 20.67 and 33.09 μg/mL and also increased the area under the concentration–time curve of hesperetin after oral administration to 16.2-and 18.0-fold, respectively. The micelles and complexes increased the solubility and remarkably improved the in vitro antioxidant activity and in vivo oral absorption of hesperetin, indicating these formulations’ potential applications in drugs and healthcare products.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Xinyang Xu,Jiawen Liu,Yixin Sun,Zhonghua Li
Among the various advanced photocatalytic materials, the organic conjugated microporous polymer (CMP) photocatalysts have garnered significant interest due to their exceptional efficiency in photogenerated electron transfer and their structural versatility. In this study, we developed two novel donor-acceptor 1 -acceptor 2 CMP systems through varying the electron donor unit. Pyrene (P) and triphenylamine (T) were acted as donor units (D), respectively, meanwhile dibenzothiophene-S,S-dioxide (BTDO, A 1 ) and 3,4-ethylenedioxythiophene (EDOT, A 2 ) as their acceptors to construct two D−A 1 −A 2 type CMP photocatalysts, P-BTDO-EDOT and T-BTDO-EDOT. Their photocatalytic performances were compared and studied through photocatalytic hydrogen production. The findings show that P-BTDO-EDOT possesses higher photocatalytic hydrogen production activity (87.21 mmol g −1 h −1 ) than T-BTDO-EDOT (66.25 mmol g −1 h −1 ) under visible light, attributing to its stable conjugated architecture, larger specific surface area, exceptional optoelectronic capability, wider light absorption, donor unit pyrene with strong electron-donating and efficient charge separation ability. This work not only highlights the potential for designing innovative CMP molecular structures with D−A 1 −A 2 system, but also opens up a new pathway for developing high-efficiency photocatalysts.
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Analytical Methods
·
2017
·Vol. 10
·期 1
·84-90
·
IF 1.9
Xia Lin,Shu Zhu,Qiao Xia,Jiao Ma,Yingzi Fu
Herein, an ultrasensitive and stereo-selective electrochemiluminescent (ECL) biosensor based on ECL signal amplification of luminol by the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires (Au-PtNWs) has been designed for the detection of D-alanine (D-Ala). In the sensor design, D-amino acid oxidase (DAAO) was used to generate the ECL co-reactant (H2O2) for luminol in situ. The reduced graphene oxide-hemin-cysteine (rGO-H-Cys) composite and Au-PtNWs acted not only as amplification labels to amplify signals via synergetic catalysis, but also as an ideal nanocarrier to accelerate electron transfer. A great difference in ECL intensities toward D-Ala and L-Ala was obtained, and an obviously higher intensity was seen for D-Ala. Under optimal conditions, the ECL intensity had a linear relationship with the logarithm of the D-Ala concentration in the range from 1.0 × 10−9 to 5.0 × 10−3 M with a detection limit of 3.3 × 10−10 M (S/N = 3). This study provides a new method for the determination of D-Ala in food with high sensitivity, excellent selectivity, and good stability and reproducibility.
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Journal of Materials Chemistry A
·
2017
·Vol. 5
·期 24
·12144-12148
·
IF 8.867
Qianqian Yang,Jie Zhou,Genqiang Zhang,Cong Guo,Meng Li,Yongchun Zhu,Yitai Qian
Sb nanoparticles encapsulated in 1-D N-doped porous carbon (denoted as Sb/NPC) have been fabricated by an in situ nanoconfined replacement reaction between SbCl3 and the intermediate Ni/NPC, in which Ni/NPC was obtained by annealing the hydrothermally synthesized nickel–nitrilotriacetic acid (Ni–NTA) precursor in an argon atmosphere. The Sb nanoparticles with a size of 10–20 nm were uniformly encapsulated in the 1-D N-doped porous carbon scaffolds. When the Sb/NPC composite was applied as an anode material in the batteries, it exhibited a high reversible capacity of 556 mA h g−1 at 200 mA g−1 after 100 cycles for Li-ion batteries (LIBs) and a reversible capacity of 400.9 mA h g−1 at 100 mA g−1 after 100 cycles for Na-ion batteries (NIBs). Such enhanced electrochemical performance of the designed Sb/NPC can be attributed to the synergistic effect between uniformly dispersed Sb nanoparticles and the 1-D N-doped porous carbon matrices.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Xinyang Xu,Yixin Sun,Jiawen Liu,Zhonghua Li
Conjugated microporous polymers (CMPs) featuring donor–acceptor (D–A) structures have garnered significant attention for their potential in hydrogen production, primarily due to their excellent charge transfer efficiency and large specific surface area. However, the uncontrollable composition in D–A structure severely limits the improvement of photocatalytic activity. In this study, employing pyrene as the donor, dibenzothiophene-S-S-dioxide (BTDO, A 1 ) and 1-methyl-1,2,4-triazole (TAME, A 2 ) as acceptors, the innovative tri-component CMP photocatalysts featuring a donor–acceptor 1 –acceptor 2 (D–A 1 –A 2 ) configuration were developed and created. By regulating the ratio of D and A, as well as BTDO and TAME in the polymer backbone, the optimized copolymer Py-BTDO-TAME-2 exhibits superior hydrogen production activity (27.53 mmol g −1 h −1 ) in the absence of Pt co-catalyst under visible light, owing to the broad light absorption, high specific surface area and enhanced charge separation capability. The findings of this study indicate that utilizing a D–A 1 –A 2 structural design method can greatly improve the photocatalytic performance of CMP photocatalysts.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Xiaoying Gao,Jiarui Zhu,Jiahui Zhao,Lei Zhao,Ying Sun,Jiazhen Lin,Mengli Hu,Yunjia Liu,Shenghong Yang,Jian Liu
A cascade colorimetric detection of salivary D-amino acids (DAAs) holds promise for the preliminary screening diagnosis of gastric cancer. Pursuing metal-organic complexes with high peroxidase-mimic (POD-mimic) activity is crucial to enhance diagnosis efficiency. In this work, we developed a straightforward strategy in a “one-pot” process to modulate the POD-mimic activity of CuX-trithiocyanuric acid (CuX-TTCA) complexes. By adjusting the molar ratios of CuX (Cl, Br, and I) and TTCA during synthesis to modify the coordination configuration of Cu(I) in CuX-TTCA, we easily tuned their POD-mimic activities. Among them, CuCl-TTCA-2 with a feeding molar ratio of 2:1 for CuCl:TTCA exhibited remarkable POD-mimic activity attributed to its exposed catalytic active sites and efficient mass transfer ability during catalysis. Long-lived 1 O 2 was identified as the primary reactive oxygen intermediate. A highly sensitive and selective cascade colorimetric detection platform was developed for two typical DAAs associated with gastric cancer, namely D-proline and D-alanine, achieving limit of detection values of 1.1 and 0.8 μM, respectively. As a proof-of-concept application, our cascade detection platform demonstrated excellent specificity in distinguishing saliva samples between gastric cancer patients and healthy individuals. The outstanding selectivity and reliable outcomes from DAAs assay make our detection platform highly promising for preliminary screening diagnosis of gastric cancer and patient self-detection applications. Our straightforward strategy for tuning POD-mimic activity provides an in-depth understanding on structure-activity relationship in nanozymes, offering valuable opportunities to advance enzyme-mimic optimization for other potential applications.
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Carbon Neutrality
·
2025
·Vol. 4
·期 1
·1-9
·
IF 12.5
Yang Delong,Dai Jinhang,Du Ziting,Cao Qingya,Chen Gang,Qi Ting,Wang Weilu,Li Fukun
Since 3-acetylamino-5-acetylfuran (3A5AF) has been regarded as a promising nitrogen-containing synthon, the conversion of renewable chitin and its monomer N-acetyl-D-glucosamine (NAG) to 3A5AF is of significance. In this paper, we compared the catalytic activity of several Cl-containing compounds and found that LiCl exhibited superior performance. The effects of solvent, catalyst dosage, reaction temperature, and reaction time on the synthesis of 3A5AF were investigated. Under the optimized conditions, about 60% yields of 3A5AF were obtained in N-methyl-2-pyrrolidone (NMP) or dimethylacetamide (DMA) medium. The spent LiCl and DMA could be recycled and reused, without obvious decrease of 3A5AF yield. Moreover, the formed 3A5AF could be purified by a facile procedure. Experiment results and density functional theory (DFT) calculations revealed that homogeneous Cl − was vital for 3A5AF formation. This study provides an example for the effective conversion of chitin biomass to value-added compounds.
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ACS Catalysis
·
2025
·
IF 13.1
Fang Huang,Aihao Xu,Xiangyu Chen,Huanhuan Sun,Siyu He,Dong Wei,Boran Wang,Anxiang Guan,Xucai Yin,Jing Xu,Huibing He
The direct electrocatalytic conversion of carbon dioxide to methane using water as a medium is a reliable way to store intermittent renewable energy and solve environmental problems. However, in such multielectron/proton transfer reactions, the role of water is often overlooked. Specifically, the sluggish kinetics of water dissociation limit the effective proton supply during methane formation. Here, we propose a copper-doped CeO2 catalyst with frustrated Lewis pairs (FLPs), which can effectively reduce carbon dioxide to methane. Combined experimental analysis and theoretical calculations reveal that the synergistic interaction between Cu and FLPs forms an f-p-d gradient orbital coupling system, which significantly promotes water dissociation to generate active protons and optimizes the adsorption behavior of the *H and *COOH intermediates. Even at the large current density of −273 mA cm–2, the Faraday efficiency of Cu/CeO2-FLPs for methane was as high as 78.0%, with a conversion frequency of 15784.1 h–1 in the flow cell. This work provides a strategy for the rational design of efficient multipoint catalytic systems.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 29
·22965-22974
·
IF 6.7
Li Mengyu,Fu Chenchen,Zhuang Wei,Zhang Keke,Xu Jingliang,Wang Zhenfu
D-Amino acid oxidase (DAAO) catalyzes the oxidation of
D-phosphinothricin
(D-PPT) to produce optically pure L-PPT, but low catalytic efficiency
limits its industrial application. Here, a synergistic strategy combining
geometric remodeling and charge engineering was applied to enhance
the oxidation activity of our previously developed stable variant
TIF-DAAO (S18T/V7I/Y132F) toward D-PPT. Systematic analysis of the
active pocket-guided alanine and neutral hydrophilic (Ser/Thr/Tyr)
scanning mutagenesis revealed position-specific steric constraints.
Subsequent Arg/His scanning tailored the positively charged microenvironment
around D-PPT, leading to a triple mutant ATR (F58A/Q335T/P221R) with
improved electrostatic complementarity and spatial fit. ATR showed
a 10.7-fold increase in catalytic activity, a 35-fold improvement
in substrate affinity, and a 380-fold higher catalytic efficiency
( k cat / K M )
toward D-PPT. Molecular dynamics simulations provided mechanistic
insight. Our results demonstrate that the synergistic optimization
of substrate tunnel geometry and the electrostatic microenvironment
effectively boosts DAAO activity, offering a rational strategy for
engineering industrial biocatalysts.
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ANALYTICAL BIOCHEMISTRY
·
2015
·
IF 2.219
Fang Wang,Wencheng Gong,Lili Wang,Zilin Chen
Selective recognition of d -tryptophan ( d -Trp) in the presence of Cu(II) was investigated at poly- l -lysine (p-l-Lys) film using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). p-l-Lys film was immobilized on a glassy carbon electrode (GCE) by cyclic voltammetry between 0.0 and 1.9 V in 20 mM phosphate buffer solution (pH 8.6). After the p-l-Lys/GCE electrode was incubated with d -Trp solution containing Cu(II) ions, obvious enhancement of electron transfer resistance and decrease of voltammetric current could be observed. If d -Trp was replaced by l -tryptophan (l-Trp), there was no apparent resistance and current changes. Moreover, no resistance and current changes could be found in the absence of Cu(II). It may be due to the formation of Cu complex with l -lysine and d -tryptophan. Finally, this method was successfully applied to monitoring enantiomeric composition of the d -Trp and l-Trp mixtures.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Pengfei Shan,Yingwen Lu,Weilin Lu,Xiangping Yin,Haiwei Liu,Daai Li,Xiaoyue Lian,Weiping Wang,Zhongyu Li,Zhihui Li
The low utilization efficiency of pesticides exerts an adverse impact on the environment and human health. Polymer-related controlled-release nanosized pesticide systems provide a promising and efficient way to overcome the problem. In this work, a biodegradable and light-responsive amphiphilic polymer was synthesized via 1,1,3,3-tetramethylguanidine-promoted polyesterification under mild conditions (low temperature, no vacuum, and no inert gas protection). We used this polymer to fabricate a light-triggered controlled-release nanosized pesticide system. The herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D), was selected as a model drug to show its potential as a controlled-release pesticide system. It was found that the 2,4-D-loaded polymeric nanoparticles were stable without the treatment of UV, while the release rate of 2,4-D from the nanoparticles gradually increased after treatment with UV light. Pot trial showed that the 2,4-D-loaded polymer nanoparticles showed a good herbicidal effect. Finally, toxicity studies suggested that the polymer can reduce toxicity to nontarget organisms.
研究领域:
环境科学
·
材料科学
·
农业科学
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