BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Ning Wang,Yiting Wang,Tingting Guo,Ting Yang,Mingli Chen,Jianhua Wang
A simple one-step hydrothermal green approach was reported for the preparation of carbon dots (CDs) without any further decoration or modification with papaya powder as natural carbon source. In this economical and eco-friendly system, deionized water or 90% ethanol was used as solvent to produce water-soluble or ethanol-soluble CDs, respectively, termed as W-CDs and E-CDs. The quantum yield (QY) for W-CDs was 18.98%, while that for E-CDs was 18.39%. The potentials of the prepared carbon dots toward diverse applications were thoroughly investigated. W-CDs and E-CDs provide promising probes for fluorescence detection of Fe 3+ , offering limits of detection of 0.48 μmol L −1 and 0.29 μmol L −1 , respectively. W-CDs was further demonstrated to be a promising probe for fluorescence sensing of Escherichia coli O157: H7, along with a limit of detection of 9.5×10 4 cfu mL −1 . Meanwhile, both W-CDs and E-CDs exhibit favorable biocompatibility, and demonstrated to be efficient for Hela cell imaging.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-10
·
IF 5.3
Duan Xiaoge,Shi Xinrui,He Zhaoyuan,Chen Hongcai,Shi Zhuanzhuan,Zhao Zhi,Chen Hailan,Yu Meiling,Guo Chunxian
Escherichia coli ( E. coli ) O157:H7 is an important food-borne pathogen that can cause hemorrhagic diarrhea and enteritis in humans and animals. Realizing the rapid quantitation of E. coli O157:H7 is of great significance for the guarantee of food safety and disease control. In this study, an electrochemical immunosensing technique based on a functionalized composite of Cu-metal organic framework (Cu-MOF) and poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS) is developed, achieving rapid and sensitive quantitation of E. coli O157:H7 in food and clinical feces samples. The organic functionalization of Cu-MOF significantly improves the interface conductivity to facilitate electron transfer and provides the sulfonic groups (–SO 3 H) to conjugate bio-recognizing elements for target determination. The immunosensor delivers a linear detection range of 3 × 10 2 ~ 3 × 10 8 cfu/mL, a low limit of detection (LOD) of 7.4 cfu/mL, and a short analysis time of 40 min. In addition, it does not show any cross-reactivity with other common pathogens and exhibits high repeatability with relative standard deviations (RSDs) all lower than 2.09%, providing a promising approach for warranting food safety and control of E. coli O157:H7 disease. Graphical Abstract
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2017
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IF 2.673
Tian-Fu He,Zhi-Hong Zhang,Xin-An Zeng,Lang-Hong Wang,Charles S. Brennan
This work was aimed to investigate the antibacterial action of cinnamaldehyde (CIN) against Escherichia coli ATCC 8735 ( E. coli ) based on membrane fatty acid composition analysis, alterations of permeability and cell morphology as well as interaction with genomic DNA . Analysis of membrane fatty acids using gas chromatography–mass spectrometry (GC–MS) revealed that the proportion of unsaturated fatty acids (UFA) and saturated fatty acids (SFA) were the major fatty acids in plasmic membrane, and their levels were significantly changed after exposure of E. coli to CIN at low concentrations. For example, the proportion of UFA decreased from 39.97% to 20.98%, while the relative content of SFA increased from 50.14% to 67.80% as E. coli was grown in increasing concentrations of CIN (from 0 to 0.88 mM). Scanning electron microscopy (SEM) showed that the morphology of E. coli cells to be wrinkled, distorted and even lysed after exposure to CIN, which therefore decreased the cell viability . The binding of CIN to genomic DNA was probed using fluorescence, UV–Visible absorption spectra, circular dichroism, molecular modeling and atomic force microscopy (AFM). Results indicated that CIN likely bound to the minor groove of genomic DNA, and changed the secondary structure and morphology of this biomacromolecule. Therefore, CIN can be deem as a kind of natural antimicrobial agents, which influence both cell membrane and genomic DNA.
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Microorganisms
·
2024
·Vol. 12
·期 9
·1871
·
IF 4.1
Lankun Yi,Mingze Cao,Xu Chen,Yubin Bai,Weiwei Wang,Xiaojuan Wei,Yuxiang Shi,Yongying Zhang,Tenghe Ma,Zhen Zhu,Jiyu Zhang
Bacterial drug resistance is becoming an increasingly serious problem, and the development of antibacterial synergists is urgently needed. Combining existing antibiotics with promising nonantibiotic agents is one strategy that has been shown to be effective at overcoming the widespread emergence of antibiotic-resistant pathogens. In this study, we investigated the antibacterial activities and mechanism of naringenin (NG) combined with amikacin (AMK) against multidrug-resistantEscherichia coli(E. coli). We first measured the fractional inhibitory concentration (FIC) of NG combined with antibiotics via the checkerboard method. The results indicated that the combination of NG and AMK had a synergistic effect onE. coliATCC 25922 andE. coliC7F3. In addition, this synergistic effect was verified by time-kill assays. Moreover, scanning electron microscopy (SEM) was used to observe cell morphology. The results showed that the cell wall ofE. coliwas destroyed. Furthermore, we assessed the leakage of alkaline phosphatase (AKP), K+, and protein. The extracellular AKP activity increased after the combinational group of 1/2MIC NG and 1/2MIC AMK, suggesting an impairment in cell wall permeability. An increase in the leakage of intracellular K+and protein indicated an increase in cell inner membrane permeability. These results revealed that NG and AMK inhibitedE. coliby damaging cell walls and membranes. In addition, PI uptake rapidly increased after treatment with NG and AMK. Confocal laser scanning microscopy (CLSM) revealed that NG caused cell wall and cell membrane damage inE. coli. In summary, our results provide a new strategy for responding to the development ofE. colidrug resistance.
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Food Bioscience
·
2024
·
IF 4.8
Cui Cao,Yiping Gao,Feilong Sun,Chunqing Ai,Shuang Song
Arabinogalactan from Lycium barbarum (LBP-3) is the major biological activity component and contributes to maintaining intestinal immune homeostasis by targeting gut microbiota and their metabolites. However, it is still a big challenge to know the specific ecological microbe-metabolite interaction regulated by LBP-3 due to the host complex diet webs and lots of unidentified intestinal bacteria. Therefore, the present study constructed a defined microbial consortium, composing of symbiotic Bacteroides caccae, Phocaeicola vulgatus , B. thetaiotaomicron , B. uniformis , B. ovatus , Lactobacillus plantarum and Escherichia coli in vitro , to investigate the impact of LBP-3 on the interactions of species and their metabolites. The utilization of LBP-3 by the defined microbial consortium of 7 intestinal species was 22.5%. Full-length 16S rRNA analysis demonstrated that LBP-3 significantly modulated the composition of synthetic bacterial community, especially inhibiting the level of opportunistic pathogenic E. coli and improving the relative abundance of probiotic L. plantarum and all Bacteroidetes species except B. ovatus . LBP-3 remarkedly influenced 27 differential metabolites, mainly belong to amino acids, peptides and analogues (37.04%) and carboxylic acid and derivatives (7.41%). Moreover, targeted metabolomics revealed that the levels of acetate, propionate, tryptophan and 5-methylthioadenosine were improved by LBP-3. In addition, LBP-3 modulated the pathways of tryptophan metabolism, cysteine and methionine metabolism, and biosynthesis of siderophore group nonribosomal peptides. Notably, some amino acids (e.g., proline and glutamate) and their related metabolic pathways (e.g., arginine and proline metabolism) altered by LBP-3 were significantly correlated with the levels of species, such as B. uniformis , P. vulgatus, and B. thetaiotaomicron .
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-11
·
IF 5.3
Han Lu,Song Yaqi,Chen Min,Pan Leiqing,Tu Kang,Su Jing
An upconversion-gold nanoparticle detection system that integrates PCR amplification and fluorescence resonance energy transfer was constructed to enable swift and highly sensitive identification of Escherichia coli . The forward primer used in the PCR amplification is modified with sulfhydryl groups, enabling its connection to gold nanoparticles via Au–S bonds. The complementary strand of the forward primer, which is attached to the upconversion nanomaterials, can hybridize with the free forward primer through base complementary pairing. This interaction induces fluorescence resonance energy transfer, resulting in fluorescence quenching. The concentration of the target bacteria influences the amount of free primer in the system after PCR amplification, which subsequently alters the intensity of the upconversion fluorescence. The fluorescent PCR sensor developed based on the aforementioned principles demonstrated a detection limit of 14 CFU/mL for E. coli , with a quantitative detection range of 18–1.8 × 10 7 CFU/mL. In comparison to the qPCR method, the number of PCR cycles required for the constructed biosensor can be reduced to approximately 22 to achieve the same detection limit, effectively decreasing the detection time by about 24 min. The spiked recoveries in chicken were 91.8–106.0% with the relative standard deviations less than 10%, indicating that the constructed method exhibits good applicability. Graphical abstract
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BIOCHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 3.7
Shu-Ping Zou,Wei Ding,Yu-Yue Han,Kun Niu,Ya-Ping Xue,Yu-Guo Zheng
L-Phosphinothricin (L-PPT) is the active compound of a broad-spectrum herbicide. In this study, we biosynthesized L-PPT via the asymmetric reduction of 2-carboxy-4-(hydroxymethylphosphoryl) butyric acid (PPO) using a whole-cell biocatalyst. Glutamate dehydrogenase from Pseudomonas moorei ( Pm GluDH), Glucose dehydrogenase from Exiguobacterium sibiricum ( Es GDH) and NAD kinase from Corynebacterium glutamicum ( Cg NADK) were coexpressed in E. coli , significantly increasing the catalytic efficiency by regenerating more NADPH to promote PPO reduction. Following the optimization of balancing the co-expression of the three enzymes in pETDuet-1 and pCDFDuet-1, the coexpressing strain increases in the intracellular NADP(H) concentration to 54.9 μmol/g DCW, which was 3.8-fold higher than the control. The co-expression system exhibited optimal activity at a temperature of 35°C, pH 7.5, and the glucose to PPO molar ratio of 1.4:1. In a 30 L bioconversion system the final concentration and productivity of L-PPT reached 79.8 g/L (>99% ee ) and 1440 g/(L·d), respectively. These results will contribute to the industrial biological production of L-PPT.
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ULTRASONICS SONOCHEMISTRY
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2024
·
IF 8.7
Changlong Li,Fengmeng Teng,Fengmin Wu,Hui Zhang,Chunbing Zhang,Dong Zhang
Sonodynamic therapy (SDT) is an emerging antibacterial therapy. This work selected hematoporphyrin monomethyl ether (HMME) as the sonosensitizer, and studied the enhanced inhibition effect of Escherichia coli and biofilm by microbubble-mediated cavitation in SDT. Firstly, the influence of microbubble-mediated cavitation effect on different concentrations of HMME (10 µg/ml, 30 µg/ml, 50 µg/ml) was studied. Using 1,3-diphenylisobenzofuran (DPBF) as an indicator, the effect of microbubble-mediated cavitation on the production of reactive oxygen species (ROS) was studied by absorption spectroscopy. Secondly, using agar medium, laser confocal microscopy and scanning electron microscopy, the effect of microbubble-mediated cavitation on the activity and morphology of bacteria was studied. Finally, the inhibitory effect of cavitation combined with SDT on biofilm was evaluated by laser confocal microscopy. The research results indicate that: (1) Microbubble-mediated ultrasound cavitation can significantly increase cavitation intensity and production of ROS. (2) Microbubble-mediated acoustic cavitation can alter the morphological structure of bacteria. (3) It can significantly enhance the inhibition of SDT on the activity of Escherichia coli and its biofilm. Compared with the control group, the addition of microbubbles resulted in an increase in the number of dead bacteria by 61.7 %, 71.6 %, and 76.2 %, respectively. The fluorescence intensity of the biofilm decreased by 27.1 %, 80.3 %, and 98.2 %, respectively. On the basis of adding microbubbles to ensure antibacterial and biofilm inhibition effects, this work studied the influence of cavitation effect in SDT on bacterial structure, providing a foundation for further revealing the intrinsic mechanism of SDT.
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Microbiology Spectrum
·
2024
·
IF 3.7
Deyi Zhao,Miran Tang,Panjie Hu,Xiaowei Hu,Weijun Chen,Zhexiao Ma,Huanchang Chen,Haifeng Liu,Jianming Cao,Tieli Zhou
Carbapenem-resistant Klebsiella pneumoniae (CRKP) and Escherichia coli (CREC) are frequently detected in clinical settings, restricting the use of carbapenems. Therefore, there is an urgent need for new antimicrobial strategies to address infections caused by CRKP and CREC. This study investigated the antibacterial, anti-biofilm, and anti-inflammatory effects of the cationic antimicrobial peptide Hs02, along with its potential antimicrobial mechanisms against CRKP and CREC. The results revealed that Hs02 had a low minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against CRKP and CREC, effectively eliminating the bacteria within 30 min. Moreover, Hs02 significantly prevents biofilm formation and disrupts the established biofilms. Further mechanistic studies demonstrated that Hs02 specifically targeted and bound to bacterial outer membrane lipopolysaccharides (LPS), disrupted membrane permeability and integrity, which led to intracellular reactive oxygen species (ROS) accumulation. Furthermore, Hs02 neutralized LPS, thereby suppressing the production of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in murine macrophage RAW 264.7 cells. In vitro, hemolysis and cytotoxicity assays confirmed Hs02’s safety at the tested concentrations and proved that Hs02 improved the survival rate of Galleria mellonella larvae. In conclusion, the findings suggest that Hs02’s interaction with LPS and the resulting disruption of membrane integrity may be key factors driving its rapid bactericidal and anti-inflammatory effects.
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Foods
·
2024
·Vol. 13
·期 13
·2143
·
IF 4.7
Shuangshuang Cui,Yong Wei,Can Li,Jian Zhang,Yunfeng Zhao,Xiayu Peng,Fengxia Sun
(1) Background: Rapid on-site testing is an effective method for the detection ofEscherichia coli O157: H7(E. coli O157: H7) in food ingredients and the environment. (2) Methods: In this study, we developed colorimetric loop-mediated isothermal amplification (LAMP) and immunochromatographic test strips (ICTs) for the rapid and visual detection ofE. coli O157: H7. This study designed new specific LAMP primers forE. coli O157: H7virulence island genes. After the LAMP amplification, the double-stranded DNA target sequence labeled with digoxin and fluorescein isothiocyanate (FITC) at both ends was bound to the anti-digoxin antibody on the gold nanoparticles. Subsequently, it was further bound to the anti-FITC antibody at the T line of the ICTs, forming a positive test result. Hydroxynaphthyl blue dye was directly added to the LAMP amplification product. A blue color indicated positive results, while a purple color indicated negative results. (3) Results: Two visualization methods showed high specificity for the target strains. The visualization tests had sensitivities of 5.7 CFU mL−1, and the detection limit of theEscherichia coli O157: H7in artificially contaminated milk samples was 5.7 × 102CFU mL−1, which was consistent with the results of the standard method (LAMP-electrophoresis method) used in commercial inspection. (4) Conclusions: Both methods could be useful in remote and under-resourced areas.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yan Zeng,Yijun Lin,Jiawei Chen,Yan Sun,Peng Qi,Peng Wang
Early detection and diagnosis are essential for effectively preventing and treating complex pathogenic bacterial infections. This study presents an innovative approach for on-site, ultrasensitive pathogen detection by integrating a uniquely designed probe binding mode with novel nanomaterial structures within a lateral flow immunoassay (LFIA) platform. Through a galvanic replacement reaction, gold is non-uniformly deposited onto a silver core, resulting in the formation of Ag@pore-Au structures. The abundant nanopores and porous surface of these structures create a high density of "hot spots", significantly enhancing their surface-enhanced Raman scattering (SERS) performance. We systematically characterized the SERS and detection capabilities of the synthesized Ag@pore-Au Raman-dye micron particles (MPs) probe. The results conclusively demonstrated that this structural probe exhibits a robust SERS signal, paving the way for its successful application in LFIA. By integrating the Ag@pore-Au Raman-dye MPs probe into the LFIA platform, we achieved rapid, quantitative detection of Escherichia coli and Staphylococcus aureus. This enhanced platform overcomes the limitations of traditional LFIA, notably its high detection limit of 10 CFU/mL and its inability to provide quantitative results. Furthermore, a dual-channel detection mode enabled the simultaneous and specific identification of both bacterial strains. The platform's efficacy was validated through successful detection in real sample matrices. This research offers novel insights and methodologies for pathogen detection in the medical field, providing an effective solution for on-site pathogen diagnosis and real-time environmental monitoring.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Laishui Yu,Liangwen Yang,Haomiao Wang,Zhuangyuan Wang,Jun Xie,Huasheng Lai,Zhengyi Chen
Ensuring rapid detection of foodborne pathogens and identifying effective natural antimicrobial agents are critical priorities for the food industry. This study presents an integrated dual-function strategy specifically designed for practical food safety applications, enabling simultaneous rapid detection of Escherichia coli ( E. coli ) in foods and screening of natural antimicrobial herbs. The developed Thermally Efficient Array Gas Membrane Separation (TAGMS) device, optimized for food sample testing conditions, allowed reliable detection of E. coli within just 18 min, significantly outperforming traditional plate-culture methods. Capable of processing up to 48 samples simultaneously, this method effectively addresses high-throughput demands typical in food production and quality control environments. The selective proliferation of E. coli was successfully achieved using selective agents (pig bile salts, amphotericin B, bacitracin), minimizing interference commonly encountered in complex food matrices. The approach leveraged ethanol—a key metabolic marker for E. coli —to provide accurate, real-time pathogen quantification. Additionally, practical evaluation demonstrated the antimicrobial efficacy of Trans-Anethole (IC 50 = 25.45 μg/mL), positioning it as a promising natural antimicrobial candidate for food preservation. Overall, this integrated analytical platform provides substantial practical benefits for food safety management and supports the adoption of natural antimicrobials to enhance consumer safety and product quality.
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