ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2025
·Vol. 69
·期 5
·
IF 4.5
Pusheng Xu,Yi Li,Bihan Xu,Dan Cao,Xu Dong,Yanghui Xiang,Xiuzhi Jiang,Xin Yuan,Yuwei Qiu,Ying Zhang
Mycobacterium abscessus is a fast-growing nontuberculous mycobacterium that can cause severe disease and poses a significant challenge for clinical management. New drugs are urgently needed for more effective treatment. Nitroxoline, an antibiotic used for treating urinary tract infections in Europe, has recently been shown to have promising activity against M. abscessus. However, the mechanism of its modified activity is not known. In anticipation for its potential clinical use, in this study, we investigated the mechanism of nitroxoline activity modification through mutant selection followed by whole-genome sequencing. We identified mutations in a transcriptional repressor MarR (MAB_2648c) controlling the expression of efflux pumps MmpS5-MmpL5 family that are associated with nitroxoline activity modification. Complementation of the mutants with the wild-type marR gene restored the MIC values to levels comparable to those of the wild-type strain. This study elucidates the key mechanism of how M. abscessus mutations modify the activity of nitroxoline, which has significant implications for rapid molecular detection of drug-resistant M. abscessus and for further studies on resistance mechanisms.
研究领域:
微生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
Redox Biology
·
2025
·
IF 11.9
Xue Wang,Xin Liu,Yumei Fan,Ke Tan,Jiaqi Gao,Yuejiao Wang,Ziyi Zhang,Shuyue Liu,Xiaofan Wang,Ke Tan,Baohua Wang,Pengxiu Cao
Pulmonary fibrosis (PF) involves persistent activation of fibroblasts and excessive deposition of extracellular matrix, with limited therapeutic options. Pulmonary iron overload has been identified in PF and is associated with the progression of PF. However, the underlying signaling pathway remains unclear. This study demonstrated that iron accumulates in the mouse lung from day 7 post-bleomycin (BLM) instillation until harvest, coinciding with the activation of pulmonary fibroblasts and the onset of fibrogenesis. Iron supplementation promoted the G 1 /S cell cycle transition and proliferation of fibroblasts, and worsened PF, whereas iron deficiency demonstrated the opposite effects. Mechanistically, both iron and reactive oxygen species (ROS) suppress Forkhead box F1 (FOXF1) expression. FOXF1 overexpression upregulates the expression of antioxidant proteins, including ferredoxin 1 (FDX1) and heme oxygenase-1 (HO-1). Both FOXF1 overexpression and FDX1 overexpression reduced cellular labile iron pool (LIP), ROS levels, and collagen synthesis in human pulmonary fibroblasts. Sonic hedgehog (SHH) signaling elevated intracellular iron, fibroblast proliferation, and its own secretion, establishing a sustained SHH/iron amplifying loop. These findings identify an iron/ROS–FOXF1 positive feedback loop and an SHH–iron self-promoting pathway that drive sustained elevated iron levels and persistent fibroblast activation and fibrogenesis, thereby deepening our understanding of the iron signaling in PF.
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DOI
PubMed
高影响因子
Cell Reports
·
2023
·
IF 8.8
Yingqi Liu,Xuemei Yao,Youbo Zhao,De Fang,Lei Shi,Li Yang,Guanbin Song,Kaiyong Cai,Liqi Li,Qin Deng,Menghuan Li,Zhong Luo
The tumor microenvironment (TME) plays decisive roles in disabling T cell-mediated antitumor immunity, but the immunoregulatory functions of its biophysical properties remain elusive. Extracellular matrix (ECM) stiffening is a hallmark of solid tumors. Here, we report that the stiffened ECM contributes to the immunosuppression in TME via activating the Rho-associated coiled-coil-containing protein kinase (ROCK)-myosin IIA-filamentous actin (F-actin) mechanosignaling pathway in tumor cells to promote the generation of TRIM14-scavenging nonmuscle myosin heavy chain IIA (NMHC-IIA)-F-actin stress fibers, thus accelerating the autophagic degradation of cyclic guanosine monophosphate (GMP)-AMP synthase (cGAS) to deprive tumor cyclic GMP-AMP (cGAMP) and further attenuating tumor immunogenicity. Pharmacological inhibition of myosin IIA effector molecules with blebbistatin (BLEB) or the RhoA upstream regulator of this pathway with simvastatin (SIM) restored tumor-intrinsic cGAS-mediated cGAMP production and enhanced antitumor immunity. Our work identifies that ECM stiffness is an important biophysical cue to regulate tumor immunogenicity via the ROCK-myosin IIA-F-actin axis and that inhibiting this mechanosignaling pathway could boost immunotherapeutic efficacy for effective solid tumor treatment.
研究领域:
癌症生物学
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免疫学
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细胞生物学
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生物物理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2025
·
IF 8.3
Mo Yan,Liang Ma,Shihao Wu,Zengyan Wei,Dechang Jia,Yu Zhou,Xiaoming Duan
Developing efficient electrocatalysts for the oxygen evolution reaction (OER) is crucial for sustainable hydrogen production via water electrolysis. Herein, we report the facile synthesis of single- or few-layer Ti 3 C 2 T x MXene and LaCoO 3 /Ti 3 C 2 T x composite catalysts by a ball milling-assisted exfoliation strategy with urea intercalation. Tafel slope plot was used to obtain the intrinsic Tafel slope of OER for LaCoO 3 /Ti 3 C 2 T x composite with varying mass ratios. By optimizing the composite ratio, the intrinsic Tafel slope of OER is decreased from 88.6 to 47.1 mV/dec, indicating a shift of rate-determining step (RDS). This enhancement stems from interfacial electronic modulation rather than conductivity and hydrophilicity provided by Ti 3 C 2 T x alone. Charge transfer between LaCoO 3 and Ti 3 C 2 T x elevated the Co valence state in LaCoO 3 and induced lattice strain, which modulated its electronic structure and shifted the RDS of OER to O–O bond formation. This study highlights the interface engineering in composite catalysts for efficient energy conversion.
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高影响因子
OPTICS EXPRESS
·
2025
·Vol. 33
·期 15
·32737-32748
·
IF 3.3
Shi Wang,Wenbo Wu,Yue Li,Chenxi Gu,Xiaolong Song,Yafang Pan,Zhen Sun,Zhen Sun,Yunfan Yang,Yunfan Yang,Tifeng Jiao,Tifeng Jiao,Mingli Wang
Surface enhanced fluorescence (SEF) plays a key role in sensing and cell imaging. However, in order to prevent cytotoxicity, the concentration of noble metal nanoparticles (Ag) needs to be lowered, which leads to poor SEF performance. Herein, different Ag@C (core-Ag, shell-carbon) nanoparticles were synthesized, and Ag@C nanoparticles prepared under 4 h heating (AC4.0) had the largest SEF enhancement factor (EFsef). Dilution of AC4.0 markedly reduces EFsef. Subsequently, Ag@C-GSH nanoparticles (ACG) were prepared by modifying glutathione (GSH) on the surface of diluted AC4.0. EFsef of ACG was improved at pH 4, and similar result for diluted AC4.0 at pH 8. This recovery of EFsef at low nanoparticle concentrations was attributed to a mechanism of electric field strength enhancement: acid-induced protonation of glutathione mediates aggregation of ACG via hydrogen bonding, which amplifies electric field strength at “hot spots”.
研究领域:
纳米技术
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光学传感
·
生物成像
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材料科学
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分析化学
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DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-15
·
IF 15.7
Cheng Yuan,Lv Lijun,Cao Zhiqiu,Huang Xiaoqing,Wang Yuqiong,Tang Yue,Gao Wen,Tang Bo
Myocardial ischemia (MI), caused by insufficient blood supply, is a pathological condition where cardiomyocytes lack oxygen and energy supply. Herein, we developed a natural photosynthetic system (HCU) consisting of chlorella pyrenoidosa ( C. pyre ), hyaluronic acid methacryloyl (HAMA) and degradable upconversion nanoparticles (UCNPs, NaCeF 4 :Yb,Tm,Zr). Upon near-infrared irradiation, HCU was photo-crosslinked in situ, thereby facilitating C. pyre photosynthetic oxygen generation within the myocardium. Concurrently, cytochrome c oxidase (CCO) in mitochondria was activated to enhance electron transport along the respiratory chain, synergistically boosting cardiac energy metabolism. Consequently, the ATP levels were elevated, and the hypoxic microenvironment was mitigated. In MI mouse models, echocardiography readings returned to normal levels, and the infarct size was significantly reduced following a 7-day treatment with HCU. Based on the photosynthetic system, this study proposes an in situ oxygen and energy metabolic regulation strategy for MI, holding certain inspiration for other ischemia diseases.
研究领域:
生物医学工程
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心血管病学
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分子生物学
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再生医学
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纳米医学
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光动力治疗
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细胞代谢
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Dongxu Shen,Yarong Liu,Chongchong Chen,Zhirong Yang,Hao Li,Shanghao Xiao,Wenjia Wu,Jingtao Wang
Precise construction of proton transfer pathways and modulation of proton carrier microenvironments are crucial for achieving high proton conduction in proton exchange membranes (PEMs). Despite extensive efforts to tailor these microenvironments, the underlying regulation of electron cloud density around proton carriers, intrinsically linked to proton conduction, remains largely overlooked. Herein, MIL-53-SO 3 H-X (X = –F, –Br, –H, –CH 3 ) nanosheets are synthesized via solvothermal coordination of Cr 3+ ions with functionalized terephthalic acid (BDC-X) ligands, followed by post-synthetic sulfonation at unsaturated Cr 3+ sites to anchor –SO 3 H groups. Density functional theory (DFT) calculations and experimental results reveal that electron-withdrawing –F groups enhance the electron cloud density around –SO 3 H sites, shortening transfer distance, aligning water molecules, and lowering the proton transfer barrier. Conversely, electron-donating –CH 3 groups deplete the electron cloud density in the –SO 3 H region, disrupting proton transport pathways. The MIL-53-SO 3 H-F nanosheet achieves an intrinsic proton conductivity of 298.3 mS cm −1 at 80 °C and 98% RH, a 4.5-fold increase over MIL-53-SO 3 H-CH 3 nanosheets (65.5 mS cm −1 ), as measured by the comb electrode. The corresponding lamellar membrane delivers a high peak power density of 734.8 mW cm −2 and remains stable for over 100 h in H 2 /O 2 PEM fuel cells (PEMFCs).
研究领域:
材料科学
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电化学
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能源材料
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纳米材料
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膜科学
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燃料电池
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Zehui Shang,Yinan Liu,Chunying Zhu,Taotao Fu,Xiqun Gao,Youguang Ma
The breakup of water-in-water droplets in a Y-junction microchannel was experimentally investigated, focusing on the unobstructed breakup mode due to its broad applicability. This process was divided into four stages: entering, squeezing, pinch-off, and filament rupture, based on the evolution of the neck width and its thinning rate. In the entering stage, neck thinning accelerates with increasing dispersed-phase flow rate Q d , droplet length l 0 , and two-phase viscosity ratio λ , and slows down with increasing continuous phase flow rate Q c . In the squeezing stage, the minimum neck width follows a power-law relation with time. The thinning rate increases with higher Q d , l 0 , and λ , whereas increasing Q c inhibits thinning. In the pinch-off stage, neck width decreases linearly with time, and the thinning is enhanced by higher Q c , Q d , and l 0 , but remains nearly independent of λ . The filament rupture stage follows a power-law thinning trend, and exhibits similar parameter dependencies, though satellite droplets may form at high Q d and Q c , and show increased instability at low viscosity. These findings offer insights into neck dynamics and interfacial rupture in low interfacial tension systems.
研究领域:
流体力学
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微流控技术
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软物质物理
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界面科学
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生物医学工程
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产品关联
DOI
高影响因子
Nano Materials Science
·
2025
·
IF 17.9
Yiming Luo,Yuan Liu,Huiling Feng,Hui Liang,Junda Shen,Yingxue Diao,Yuanchao Liu,Xiaoliang Zeng,Zhenwei Yu,Rong Sun,Binbin Zhou
The increasing power density of chips poses a significant challenge in the form of material aging for aluminum-filled polydimethylsiloxane (Al/PDMS) composites, which are widely used in thermal interface materials. Despite the growing importance of this issue, the specific mechanisms behind the interfacial aging process remain elusive, hindering a comprehensive grasp of the aging dynamics in these composites. In our research, we have developed an in-situ Raman aging monitoring system that leverages the non-contact and high-resolution capabilities of Raman spectroscopy to study the interface aging process. Our findings indicate a notable decrease in the intensity of the Raman peak as further cross-linking of the molecules during aging, with the most pronounced decline occurring at the interface between aluminum and PDMS. This insight could potentially elucidate why the interface in composite materials is frequently the site of failure during aging. Our study offers a versatile methodology for investigating the interfacial aging of polymer composites, contributing to a broader understanding of the interface behavior in composite materials at the molecular level.
研究领域:
材料科学
·
物理化学
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DOI
高影响因子
Device
·
2025
·
IF 8
Weikang Wang,Yan Bai,Ning Ding,Zhen You,Weiwei Zhao,Pin Yang,Mingli Liang,Shujuan Liu,Qiang Zhao
Photo-assisted flexible supercapacitors have emerged as transformative power solutions by integrating with solar photoirradiation-enhanced capacity (PIEC) to improve energy storage performance. However, the photogenerated carrier recombination and structural instability of existing metal oxides and carbon-based photoelectrodes hinder enhancement of PIEC performance in flexible supercapacitors for wearable electronics. Herein, we propose a dynamic bonding strategy to construct a core-shell covalent organic framework/MXene heterostructure (COF-316-Ti 3 C 2 T x ) with engineered heterointerfaces for flexible photo-enhanced micro-supercapacitors (FPMSCs). This method facilitates the oriented growth of covalent organic framework-316 (COF-316) on a Ti 3 C 2 T x nanosheet to form a core-shell heterostructure by anchoring monomers. Theoretical calculations and experiments reveal that the built-in electric field modulation accelerates photogenerated electron transfer and suppresses charge recombination (carrier lifetime is doubled). The core-shell heterostructure endows the realization of FPMSCs with enhanced mechano-electrochemical stability, including 180° bending stability and 97.5% of capacitance retention after 10,000 cycles.
研究领域:
材料科学
·
纳米科技
·
电化学
·
能源存储
·
柔性电子学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2022
·Vol. 32
·期 16
·2109173
·
IF 19.924
Jie Deng,Deqing Lin,Xiangyu Ding,Yuan Wang,YuHan Hu,Hui Shi,Lin Chen,Bingyang Chu,Lei Lei,Chunmei Wen,Jiaqing Wang,Zhiyong Qian,Xingyi Li
Peptide-drug conjugates (PDCs) are an important class of therapeutic agents that combine one or more active molecules with a short peptide via a biodegradable spacer to generate prodrugs. Here an antioxidant (i.e., 3,5-dihydroxybenzoic acid), a non-steroidal anti-inflammatory drug (i.e., ibuprofen, IPF), and a self-assembled peptide motif (i.e., Gly-Phe-Phe-Tyr-Asp, GFFYD) are integrated to produce a peptide-drug amphiphile, which self-assembles to form hydrogel and further exerts the synergistic effects, and consequently emerges high therapeutic efficacy. In the in vitro study toward lipopolysaccharide-activated RAW264.7 macrophages, the resulting hydrogel significantly reduces the production of cytokines (e.g., IL-1β, IL-6 and tumor necrosis factor-α (i.e., TNF-α)) compared with that of native IPF via the inhibition of nuclear factor-κB (i.e., NF-κB), janus kinase-signal transducer and activator of transcription (i.e., JAK-STAT) signals and NLR family pyrin domain containing 3 (i.e., NLRP3) inflammasome activation. Moreover, a rabbit model of endotoxin induced uveitis evidences the superior therapeutic efficacy of the resulting hydrogel over that of clinically used 0.1 wt% diclofenac sodium eyedrop. Overall, this work offers new insights into the anti-inflammatory mechanism of PDCs hydrogel and presents the therapeutic values in the treatment of ocular disorders, and ultimately fulfils the potential clinical benefits of PDCs.
研究领域:
药学
·
材料科学
·
免疫学
·
眼科学
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DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2026
·
IF 5.1
Miao Qin,Tao Liu,Yimin Zhang,Hong Liu,Ling Ge
H 2 SO 4 -HCl mixed-acid vanadium electrolytes broaden the operating window and allow higher vanadium concentrations, yet the negative V 3+ /V 2+ reaction can remain kinetically sluggish and impose substantial polarization. Here, a small amount of BiCl 3 is introduced directly into the mixed-acid negative electrolyte so that metallic bismuth forms and deposits in situ on graphite felt during cell operation, without electrode pretreatment. UV–vis spectra show that adding BiCl 3 does not measurably change the characteristic V 3+ absorption features, indicating that the bulk vanadium speciation remains unchanged. After charging, plate-like Bi 0 deposits are observed on the carbon fibers, and the V 3+ /V 2+ redox reaction is clearly accelerated. Electrochemical measurements show that 0.02 mol·L −1 Bi 3+ is most effective. The redox peak separation decreases from 535.19 mV to 212.35 mV, and the charge-transfer resistance is substantially reduced. The exchange current density increases from 0.195 mA·cm −2 to 0.324 mA·cm −2 , and the standard rate constant increases from 2.021 × 10 −9 cm·s −1 to 3.358 × 10 −9 cm·s −1 . By comparison, the double-layer capacitance increases only from 0.572 mF·cm −2 to 0.641 mF·cm −2 , suggesting that the gain mainly comes from faster interfacial charge transfer rather than a simple increase in electrochemically accessible area. In single-cell tests from 40 to 160 mA·cm −2 , the Bi-containing electrolyte shows lower polarization and improved voltage and energy efficiencies.
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高影响因子
危险品化学品经营许可证（带存储）