查找使用阿拉丁产品的科研文献

Collective influence of substrate chemistry with physiological fluid shear stress on human umbilical vein endothelial cells

Yan Li,Zhongjie Qin,Lin Zhou,Khawar Ali Shahzad,Delin Xia

In the treatment of cardiovascular diseases, vascular scaffold materials play an extremely important role. The appropriate substrate chemistries and 15 dynes/cm 2 physiological fluid shear stress (FSS) are both required to ensure normal physiological activity of human umbilical vein endothelial cells (HUVECs). The present study reported the collective influence of substrate chemistries and FSS on HUVECs in the sense of its biological functions. The CH 3 , NH 2 , and OH functional groups were adopted to offer a variety of substrate chemistries on glass slides by the technology of self-assembled monolayers, whereas FSS was generated by a parallel-plate fluid flow system. Substrate chemistries on its own by no means had noticeable effects on eNOS, ATP, NO, and PGI 2 expressions, while FSS stimuli enhanced their production. While substrate chemistries, as well as FSS, were both exerted, the releases of ATP, NO, and PGI 2 were dependent on substrate chemistries. Study of F-actin organization and focal adhesions (FAs) formation of HUVECs before FSS exposure proves that F-action organization and FAs formation followed similar chemistry-dependence. Hereby proposed a feasible mechanism, that is, the F-actin organization and FAs formation of HUVECs are controlled by substrate chemistries, further advancing the modulation of FSS-triggered responses of HUVECs.

Tannic Acid-Fe-Functionalized Mesoporous Polydopamine Nanoplatform for Synergistic NO/Calcium Overload-Enhanced Ferroptosis and Photothermal Therapy in Breast Cancer

Xueqi Liang,Yingying Cai,Zhen Liu,Nan Wang,Ahmed Mohamed Omer,Junhong Ling,Xiao−kun Ouyang

Ferroptosis, an iron-dependent form of regulated cell death characterized by lipid peroxidation and redox imbalance, has emerged as a promising strategy for treating drug-resistant cancers. However, its therapeutic efficacy is often limited by the antioxidant-rich tumor microenvironment (TME), which inhibits reactive oxygen species (ROS) accumulation. In this study, we introduced a tumor-responsive nanoplatform (MLCT) designed to synergistically amplify ferroptosis through a combination of iron catalysis, calcium overload, nitric oxide (NO) release, and photothermal stimulation. The MLCT platform consisted of mesoporous polydopamine (MPDA), calcium peroxide (CaO2), l-arginine (LA), and a tannic acid-Fe3+ (TA-Fe) shell, facilitating TME-responsive release of therapeutic agents. In vitro, MLCT effectively depleted glutathione (GSH) and sustained NO generation, resulting in elevated ROS levels and mitochondrial dysfunction. Additionally, upon near-infrared (NIR) irradiation, localized hyperthermia further potentiated ferroptotic activity. In vivo, MLCT combined with NIR treatment resulted in an 86.34% reduction in tumor growth, with minimal systemic toxicity. These results highlighted the potential of MLCT as a precision-engineered ferroptosis platform for enhanced cancer therapy.

Simultaneous Removal of Elemental Mercury and NO from Simulated Flue Gas at Low Temperatures over Mn–V–W/TiO2 Catalysts

Jialin Meng,Yufeng Duan,Peng Hu,Yifan Xu,Xinze Geng,Ting Yao,Shaojun Ren,Hongqi Wei

A series of Mn–V–W/TiO2 (Mn–VWT) catalysts were synthesized by the impregnation method and calcinated at different temperatures (300–600 °C). To explore the effects of the reaction temperature, SO2, and H2O on the simultaneous removal performance of elemental mercury (Hg0) and NO, Mn–VWT were investigated by a fixed-bed reaction system. Various techniques (scanning electron microscopy, Brunauer–Emmett–Teller, X-ray diffraction, H2-temperature-programmed reduction, NH3-temperature-programmed desorption, and X-ray photoelectron spectroscopy) were utilized to characterize the samples. The results showed that the catalytic activity of Mn–VWT first increased and then decreased with the increasing calcination temperature. The removal efficiency of Hg0 and NO still had good performance even in low reaction temperature (200 °C), achieving 100 and 82%, respectively. Mn–VWT-400 had the best simultaneous removal performance because of the highest content of the Mn4+, V4+, and chemisorbed oxygen Oα and the best reductive activity at low temperature. Furthermore, SO2 irreversibly inhibited Hg0 and NO removal ability due to the sulfate and sulfite generation on the surface of Mn–VWT-400. Especially, the inhibition effect was more serious if SO2 and H2O coexisted because the rate of sulfate/sulfite was higher than that of the former. In addition, with the increase in reaction temperature, the effect of SO2 and H2O on the removal of Hg0 and NO over Mn–VWT was gradually reduced.

Coexistence of enhanced Hg0 oxidation and induced Hg2+ reduction on CuO/TiO2 catalyst in the presence of NO and NH3

Hailong Li,Weilin Zhang,Jun Wang,Zequn Yang,Liqing Li,Kaimin Shih

Simultaneous nitrogen monoxide (NO) reduction and elemental mercury (Hg 0 ) oxidation in the presence of ammonia (NH 3 ) was for the first time studied on a copper oxide/titanium dioxide (CuO/TiO 2 ) catalyst at low flue gas temperatures. More than 80% NO reduction and 90% Hg 0 oxidation were concurrently achieved on the CuO/TiO 2 catalyst at 250   °C under a simulated NH 3 selective catalytic reduction (SCR) atmosphere. NO was likely oxidized on the CuO/TiO 2 catalyst to form active nitrogenous species, particularly with the aid of oxygen. The active nitrogenous species then significantly promoted Hg 0 oxidation. NH 3 itself did not affect Hg 0 conversion on the CuO/TiO 2 catalyst. The co-occurrences of NO and NH 3 led to a reduction in oxidized mercury (HgO in this work), which partially offset the Hg 0 oxidation and therefore resulted in a relatively lower Hg 0 conversion efficiency. However, compared to flue gas without NO and NH 3 , the combined presence of NO and NH 3 still enhanced Hg 0 oxidation over the CuO/TiO 2 catalyst, primarily because of the overwhelming benefit from NO. This is superior to many other SCR catalysts, including commercial vanadia-based SCR catalysts, on which an SCR atmosphere generally inhibits Hg 0 conversion.

Constructing hollow silkworm structure in MnOx–TiO2 catalysts for improving the performance in selective catalytic reduction of NO by NH3

Xie Chong,Yang Shenghui,Shi Jian-Wen,Niu Chunming

A novel MnO x –TiO 2 catalyst with hollow silkworm structure was synthesized successfully via an ethanol-thermal strategy by using activated carbon fiber felt as template for the first time. The silkworm-like MnO x –TiO 2 catalyst (MnTi) exhibited higher NO x conversion in the whole experimental temperature region (90–330 °C) under a high space velocity of 36,000 h −1 in comparison with the common MnO x –TiO 2 catalyst without hollow silkworm structure (MnTi-B). The excellent catalytic performance of MnTi can be attributed to its high specific surface area, large content of active surface oxygen species (O S ), and more surface acid sites. In addition, the MnTi catalyst also exhibited excellent resistance to water or SO 2 poisoning.

Promotional effect of phosphorus addition on improving the SO2 resistance of V2O5-MoO3/TiO2 catalyst for NH3-SCR of NO

Li Huang,Yiqing Zeng,Yibo Gao,Hu Wang,Yuhao Zong,Zhengfeng Chang,Shule Zhang,Pei Han,Yang Yu

The oxidation of SO 2 into SO 3 promote the formation of NH 4 HSO 4 (ABS), which can cover surface active sites catalyst, and then leading to the decrease of NH 3 -SCR De-NO x activity. In this work, phosphorus species were loaded on the V 2 O 5 -MoO 3 /TiO 2 De-NO x catalyst to enhance its SO 2 resistance via inhibiting the SO 2 /SO 3 conversion. XRD and N 2 -adsorption results showed that phosphorus has little impact on the structure and pore property of the catalyst. The results of XPS , H 2 -TPR, and NH 3 -TPD analyses illustrated that suitable amount of phosphorus (1%) increases the content of polymeric vanadate and chemisorbed oxygen (O α ) of the catalyst, which is benefit to the SCR process. Phosphorus species decreases the SO 2 absorbability and SO 2 oxidation activity of V-Mo-P/Ti catalysts, thus it exhibits higher NH 3 -SCR activity than that of the P-free catalyst at presence of SO 2 due to the lower SO 2 /SO 3 conversion and fewer amount of NH 4 HSO 4 deposition.

Dual-dynamic carboxymethyl chitosan hydrogel with photothermal properties and programmed radical/NO release properties for enhanced healing of infected wounds

Hongchao Deng,Zikang Wang,Shoumiao Peng,Shunyu Yao,Yiwang Zhao,Huiling Guo

Bacterial-infected wound healing still faces substantial challenges, including multidrug-resistance, uncontrollable bleeding, long-term inflammation, and impaired angiogenesis. However, current treatment strategies cannot meet the complex demands of skin wound healing. We here developed a dual dynamic CFP hydrogel based on Schiff base and borate ester bonds between carboxymethyl chitosan (CMCS), polyvinyl alcohol (PVA), and 2-formylphenylboronic acid (2-FPBA). Meanwhile, customized L-arginine/epigallocatechin-3-gallate/ferric ion (EAF) nanoparticles and the thermal-labile 2,2′-azobis[2-(2-imidazolin-2-yl) propane] dihydrochloride (AIPH) initiator were embedded into the CFP hydrogel for healing infected wound. The EAF/A/CFP hydrogel could create a protective barrier on bleeding wounds and reduced blood loss. Under 808 nm laser irradiation, the hydrogel exhibited excellent photothermal, alkyl free radical production ability, and continuous release of nitric oxide (NO) to effectively eradiate the bacteria, including mature biofilms. In addition, NO release regulated inflammation and promoted angiogenesis, thus significantly promoting the wound healing. The EAF/A/CFP hydrogel successfully achieved rapid wound repair in a full-thickness skin wound model. Consequently, this study provides a potential dressing for comprehensive management of skin wound healing.

NO-Responsive Oleanolic Acid Self-Assembled Micelles Co-Loaded with BAY 11-7082 for Synergistic Chondroprotection and Anti-Osteoarthritis Therapy

Dandan Zhang,Zhigang Zhang,Dingxing Huang,Zhuoran Sun,Jiamin Huang,Chi Zhang,Qingyang Zeng,Qiling Liu,Wenzhuo Chen

Osteoarthritis is an irreversible degenerative joint disease driven by sustained NF-κB-mediated inflammatory responses, and conventional intra-articular hyaluronic acid or small-molecule NF-κB inhibitors cannot achieve targeted on-demand treatment due to poor solubility, rapid clearance and lack of lesion microenvironment responsiveness. OA with inherent anti-chondrolytic activity can self-assemble into nanocarriers in water, yet it lacks stimuli-responsive capacity. Herein, we rationally designed and synthesized an OA-Der by covalently conjugating o-phenylenediamine fragments to the OA backbone.1H NMR and HRESI-MS spectra fully verified the accurate chemical structures of intermediate and final OA-Der. Blank OA-Der micelles exhibited uniform spherical core–shell nanostructures (50–150 nm) under TEM and AFM, while pathological high NO triggered complete disassembly of micellar assemblies. We further co-assembled OA-Der with NF-κB inhibitor BAY 11-7082 to construct NO-responsive BAY@OA-Der supramolecular micelles. In vitro experiments using human C28/I2 chondrocytes with LPS-induced inflammatory injury demonstrated that BAY@OA-Der significantly improved cell viability and reduced apoptotic chondrocyte proportion. At mRNA and protein levels, the supramolecular micelle formulation remarkably suppressed NF-κB p65 phosphorylation, downregulated cartilage-degrading ADAMTS5, and upregulated ACAN compared with free BAY or blank OA-Der. Collectively, this natural bioactive self-assembled NO-responsive delivery platform achieves synergistic anti-inflammatory and matrix-protective effects by precisely releasing drugs at NO-overexpressed osteoarthritis inflammatory sites and offers an in vitro design strategy for osteoarthritis responsive delivery systems.

Engineering a Haversian-gyroid biomimetic scaffold: NIR-triggered photothermal-NO cascade antibacterial, hydroxyapatite enhanced pH balance and cell osteogenic differentiation

Huixing Li,Jiaxing Xiong,Fangwei Qi,Jun Zan,Xiuwen Gao,Wenjie Ma,Kunlong Li,Gao Pan,Pan He,Dongan Wang,Yang Shuai,Shuping Peng

Biomimetic hierarchical bone scaffold: outer Haversian canals and inner Gyroid pores for high memchanical strength. • Cascade antibacterial: thermal cleaves biofilms and Fe-NO bonds, releasing NO into bacteria to kill S. aureus and E. coli • Synergistic osteogenesis: thermal activates HSP70, synergizing with Ca 2+ and PO 4 3- to boost osteogenic differentiation. • pH regulation: amine groups from oxidative self-polymerization increase the pH value, enhancing scaffold stability. Poly- L -lactic acid (PLLA) is widely used in bone scaffold engineering due to its favorable biocompatibility, biodegradability, and processability. However, its mechanical properties are limited by insufficient crystallinity during fabrication. Moreover, bone repair is often accompanied by early bacterial infection and local pH reduction, which challenge the antibacterial effect, osteogenic capacity and structural stability of PLLA scaffolds. Herein, PLLA and hydroxyapatite (HA) are melt-blended into PLLA/HA (PHA). followed by self-polymerization of polydopamine (PDA) and electrostatic adsorption sodium nitroprusside of (SNP). The composite is then prepared into a PHA@PDA-SNP scaffold, with an outer Haversian canal layer and inner Gyroid-perforated. Ingeniously, the scaffold utilizes photothermal produced by near-infrared light to cleave biofilm and the Fe-NO bond in SNP, promoting NO release and entry into bacteria. Meanwhile, thermal can activate HSP70, synergizing with Ca 2+ and PO 4 3- released by PHA to boost osteogenic differentiation. Moreover, the amine groups retained during the oxidation process of PDA can weaken the acidic environment and enhance scaffold stability. Results show that the bone scaffold mimics the natural bone structure with high compressive strength (162.9 MPa) by biomimetic manufacturing. Scaffold exhibits cell osteogenic differentiation as evidenced by a 70% upregulation of HSP70 and Ca 2+ influx, and a 4.5-fold increase of ALP and ARS. Scaffold utilizes photothermal and NO to achieve antibacterial rates of 97.4% and 98.2% against S. aureus and E. coli , respectively. Download: Download high-res image (194KB) Download: Download full-size image

The heterojunction regulated by Au nanoparticles efficient removal of NO through the synergistic effects of SPR and the electron bridge

Youzhou He,Zhiqiang Guo,Yue Li,Xingyan Liu,Kaili Wu,Yuyu Fang,Rong Peng,Min Fu,Bowen Lei

The elimination of low-concentration (ppb) nitrogen oxides (NO x ) in city and indoor environments, particularly the selective removal of NO while preventing toxic NO 2 formation is a significant challenge. Photocatalytic technology offers a promising solution, but its effectiveness is limited by high carrier recombination rates and insufficient active sites. This study introduces a photocatalyst (Au@NM/ZIF) that forms a Z-type heterojunction structure by precisely positioning gold nanoparticles within the NM/ZIF framework. The catalyst consists of amino-functionalized NH 2 -MIL-125 and ZIF-8, with gold nanoparticles serving dual roles: UV-Vis analysis shows that their surface plasmon resonance (SPR) enhances visible light absorption, promoting the generation of charge carriers that drive the oxidation of NO to NO 3 − . Additionally, X-ray photoelectron spectroscopy (XPS) and work function analysis indicate that gold nanoparticles function as an electronic bridge, optimizing charge transfer between NM and ZIF-8, improving electron-hole separation and increasing active site utilization, thereby significantly enhancing NO conversion efficiency. In situ Fourier-transform infrared spectroscopy (FT-IR), electron spin resonance (ESR), and radical capture experiments further clarify the formation pathways of preliminary products and completed outputs, emphasizing the important roles of active radicals (such as •OH and •O 2 − ) in the deep oxidation of NO. This research offers a valuable solution for effectively removing low-concentration NO x .

Synergistic effect of cobalt and niobium in Co3-Nb-Ox on performance of selective catalytic reduction of NO with NH3

Zhang Bo-Lin,Deng Li-Feng,Liu Bo,Luo Chun-Yun,Liebau Michael,Zhang Shen-Gen,Gläser Roger

Abstract Combining the redox properties of Co and the acid properties of Nb in a Co 3 -Nb-O x catalyst is shown to provide superior performance in the selective catalytic reduction of NO with NH 3 (NH 3 -SCR). Co 3 O 4 shows average activity, however, it exhibits a poor N 2 selectivity. Nb 2 O 5 is not active for NH 3 -SCR. However, the mixed Co 3 -Nb-O x catalyst shows higher NO conversion and N 2 selectivity than the single Co 3 O 4 and Nb 2 O 5 catalysts at 100–300 °C. The results of temperature programmed reduction by H 2 and X-ray photoelectron (XP) spectra indicate that the addition of Nb changes the chemical states of Co and decreases the concentration of Co 3+ and O α , adjusting the activity for catalytic oxidation to a moderate level. This suppresses the formation of undesired N 2 O from the over-oxidation of NH 3 . Incorporation of Co and Nb into one solid synergistically couples their redox behavior and surface acidity, ensuring the high catalytic activity and N 2 selectivity in NH 3 -SCR. Graphical Abstract

Synergistic charge redistribution at SrTiO3@NH2-MIL-125 S-scheme heterostructure: steering photogenerated reactive species for efficient NO-to-nitrate conversion

Wenrui Dai,Chenxiang Wang,Jiajia Gao,Haotong Ma,Yingchun Miao,Shuo Wang,Zhanshuo Tao,Shuning Xiao