Journal of Food Measurement and Characterization
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2022
·Vol. 16
·期 6
·4486-4496
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IF 3.006
Feng Xian,Wu Zufang,Weng Peifang
Elderberry fruits are rich in nutrients and health-promoting bioactive compounds, meaning they are ideal substrate for lactic acid bacteria (LAB) fermentation. However, there is a lack of study on the compounds change of elderberry juice (EJ) fermented by LAB. This research used untargeted metabolomics to evaluate the changes of metabolites of EJ after fermented by Lactobacillus bulgaricus BNCC336436 and Streptococcus thermophilus ABT-T. Multivariate statistical analysis was applied to investigate significantly different between the unfermented and fermented EJ ( p < 0.05). Subsequently, 86 marker metabolites were identified by LC–MS/MS. The results showed that 16 phenolic compounds, 15 amino acids and derivatives, 11 sugars and derivatives, 10 fatty acids and derivatives, 6 organic acids and 28 other compounds were identified as marker metabolites between unfermented and fermented EJ. The content of total phenolic, total amino acids and derivatives tented to increase after fermentation, whereas the content of sucrose, l -fucose, l -malic acid, tartaric acid and citric acid decreased. The bioactive compounds of EJ were changed after lactic acid bacteria (LAB) fermentation, which provided a new choice for the development and utilization of EJ in the future.
研究领域:
食品科学
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代谢组学
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微生物学
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高影响因子
SOLAR ENERGY
·
2024
·
IF 6
Junhan Xu,Binghong Chen,Kunpeng Yuan,Jun Shu,Qiguo Yang
Ag@SiO 2 nanofluid is widely used in spectral splitting PV/T system. Its core–shell structure has great influence on the optical properties. In this work, we focus on the comprehensive analysis and structure optimization of Ag@SiO 2 nanofluid to achieve its optimal spectral performance. DDA method is used to predict the optical properties of Ag@SiO 2 nanofluid and an optimization model based on filter efficiency is proposed. The effect of the SiO 2 shell thickness and Ag core mass concentration is analyzed. It indicates that the spectral performance of Ag@SiO 2 nanofluid can be improved with SiO 2 shell thickness of 15–40 nm and Ag core mass concentration of 81–135 mg/L. To achieve the same theoretical merit function of 1.46, the usage of Ag mass can be reduced by 25/33/44/62 % with SiO 2 coating of 10/20/40/70 nm. The optimal structure to achieve the highest filter efficiency η of 37.8 % is with a shell thickness of 20 nm and a mass concentration of 113.9 mg/L. An indoor PV/T operation testing is conducted to verify the optimization results. The merit function of Ag-based nanofluids increases from 1.58 to 1.598 and a reduction in Ag usage of 17 % is achieved with a SiO 2 coating shell of 17.8 nm. Operation stability is also enhanced with no aggregation observed during the working cycle and 7-day static experiment.
研究领域:
材料科学
·
能源工程
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OPTICAL MATERIALS
·
2020
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IF 2.779
Jianqing Lin,Xing Ju,Chao Xu,Yu Yang,Xiaoze Du
Spectral beam splitting technology based on optical nanofluids (NFs) is a promising field of solar energy research. However, the challenges to optimize the optical properties and enhance the high temperature stability of NFs are always exist. In this study, Indium Tin Oxide (ITO) nanoparticles were selected as the research object because of the excellent optical properties. The Oleylamine-capped ITO nanoparticles were prepared by chemical reduction. A novel NF based on Therminol 66 was successfully prepared. The high temperature stability and optical properties of obtained Oil-based NFs were analyzed by the gravitational sedimentation method and spectrophotometry . Moreover, we experimentally studied the effect of single reaction condition on the morphology and Sn-doping of particles. The results indicated that, at the reaction condition of 320 °C, 5.5 h, and the In/Sn mole ratio of 90:10, the obtained nanoparticles had high monodispersity and crystallinity , and the resulting NF had good stability and dispersion. Furthermore, when tuning single reaction condition, the morphology and Sn doping of nanoparticles tended to be a regular change. However, the change of single reaction condition was not enough to obtain the ITO NFs with good stability and dispersion. The ITO NFs obtained in present study had high thermal stability, and showed different high-temperature characteristics in the Ultraviolet and visible (UV–Vis) and near infrared (NIR) regions.
研究领域:
材料科学
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太阳能技术
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纳米科技
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高影响因子
PLoS Pathogens
·
2026
·Vol. 22
·期 3
·e1014042
·
IF 4.9
Yushu He,Yang Wang,Xiaoying He,Qingbo Lv,Isabelle Vallee,Pascal Boireau,Jing Ding,Xiaolei Liu
Trichinella spiralis ( T. spiralis ), a zoonotic nematode that causes severe myositis and systemic morbidity, sustains chronic muscle parasitism through evolutionary adaptations; however, this globally prevalent disease lacks targeted therapies to disrupt chronic infection. Although the heme transport protein HRG-1 has been characterized as an intervention target in free-living species (e.g., Caenorhabditis elegans ) and hematophagous parasites (e.g., Haemonchus contortus ), the molecular machinery governing heme acquisition in the nonhematophagous parasite T. spiralis remains uncharacterized, and no drugs targeting HRG-1 have been reported until now. Herein, we demonstrate that T. spiralis , a parasite that lacks the ability to synthesize heme autonomously, has evolved a sophisticated mechanism to scavenge and utilize heme from its host. By employing an aspartic protease to degrade host hemoglobin and myoglobin in the parasitic niche, T. spiralis is able to liberate heme for its own growth and survival. The structurally and functionally conserved Ts -HRG-1 protein plays a key role in transporting heme to the entire worm, particularly to functional organs, such as the cuticle and stichosome. More importantly, we discovered that the interaction between Ts -HRG-1 and Ts -ATP6V0C results in the formation of a functional complex that is essential for the parasite’s heme acquisition. The intervention effect achieved by Ts -ATP6V0C RNAi or inhibiting the activity of Ts -ATP6V0C with bafilomycin A1 (BafA1) was consistent with Ts -HRG-1 RNAi, resulting in impaired heme uptake, developmental arrest and a reduced larval burden in mouse hosts. These findings enhance our understanding of the parasite’s heme acquisition mechanism and identify the development of drugs that target proteins that interact with HRG-1 as a new direction in anthelminthic drug research.
Chronic Trichinella spiralis ( T. spiralis ) infections currently lack effective anthelmintic drugs, particularly for the persistent muscle-dwelling phase. Exploring the physiological activities of T. spiralis during muscle parasitism as a breakthrough for drug development, we discovered that the T. spiralis aspartic protease degrades myoglobin to release heme, just as it degrades hemoglobin. As an organism incapable of synthesizing heme de novo, protein molecules that regulate heme uptake are undoubtedly potential intervention targets. Based on previous studies, we functionally confirmed that structurally conserved HRG-1 is a key protein involved in heme uptake in T. spiralis . Although reducing HRG-1 expression via RNAi affects larval growth and development, notably, no drugs blocking HRG-1 have been developed to date. In this work, Ts -ATP6V0C was identified as an HRG-1-interacting protein through coimmunoprecipitation, and most importantly, the intervention effect achieved by pharmacological inhibition of Ts -ATP6V0c was identical to that of RNAi. These findings suggest that the development of inhibitors targeting HRG-1-interacting proteins represents a new therapeutic direction for T. spiralis infections and even helminth infections.
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JOURNAL OF CHEMICAL RESEARCH
·
2023
·
IF 1.4
Zhe Chen,Yunxia Wang
A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes is developed to sensitively and specifically detect telomerase activity. Thioflavin T has a dual role as an efficient inducer and fluorescent probe, and the incorporation of thioflavin T into the thioflavin T–induced G-quadruplexes results in an intense fluorescence enhancement. In the presence of thioflavin T and K+, G-quadruplexes are formed by elongation of the telomerase substrate primer that is catalyzed by telomerase extracted from cancer cells. Thus, the telomerase activity in cancer cell extracts can be evaluated by measuring the thioflavin T fluorescence. More importantly, thioflavin T can specifically recognize and bind to G-quadruplexes, whereas it cannot recognize single- and double-stranded DNAs, which leads to the thioflavin T–based fluorescence assay exhibiting a reduced background and improved signal-to-noise ratio. As a result, the proposed assay has the linear range from 5 to 200 HeLa cells and the detection limit is 34 HeLa cells, which holds great potential for use in the detection of telomerase activity and the diagnosis of cancer.
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Frontiers in Immunology
·
2023
·
IF 7.3
Xi Zhang,Tiaoxia Wang,Xiaona Zhu,Yong Lu,Mingpeng Li,Zhihong Huang,Deping Han,Longzhen Zhang,Yang Wu,Liantao Li,Frank Klawonn,Renata Stripecke
Introduction Epstein-Barr virus (EBV) is a widely spread pathogen associated with lymphoproliferative diseases, B/ T/ NK cell lymphomas, nasopharyngeal carcinoma (NPC) and gastric carcinoma (GC). EBV lytic reactivations contribute to the genomic instability, inflammation and tumorigenesis of NPC, promoting cancer progression. Patients with NPC refractory to standard therapies show dismal survival. EBV gp350 is an envelope protein detectable in NPC specimens intracellularly and on the cell membrane of malignant cells, and is a potential viral antigen for T cell-directed immunotherapies. The potency of T cells engineered with a chimeric antigen receptor (CAR) targeting gp350 against EBV + lymphoproliferative disease was previously shown. Methods Here, we advanced towards preclinical and non-clinical developments of this virus-specific CAR-T cell immunotherapy against NPC. Different gp350CAR designs were inserted into a lentiviral vector (LV) backbone. Results A construct expressing the scFv 7A1-anti−gp350 incorporating the CD8 transmembrane and CD28.CD3ζ signaling domain (ZT002) was selected. High titer ZT002 (~1x10 8 TU/ml) was manufactured in HEK 293T/17 suspension cells in serum free media as large-scale production under good manufacturing practices (GMP). A LV multiplicity of infection (MOI) of 1 resulted in high frequencies of functional gp350CAR + T cells (>70%) at a low (<2) vector copy numbers in the genome. ZT002 was therefore used to establish gp350CAR-T batch run production methods. GMP upscaling and validation of T cell transduction and expansion in several runs resulted in average 3x10 9 gp350CAR-T cells per batch. >80% CD3 + gp350CAR-T cells bound to purified gp350 protein. In vitro cytotoxicity and cytokine secretion assays (IFN-γ and TNF-α) confirmed the specificity of gp350CAR-T cells against gp350 + NPC, GC and lymphoma cell targets. Immunocompromised B-NDG mice (NOD.CB17- PrkdcscidIl2rgtm1 /Bcgen) were challenged s.c. with a EBV + NPC C666.1 cell line expressing gp350 and then treated with escalating doses of gp350CAR-T cells or with non-transduced T cells. gp350CAR-T cells promoted antitumor responses, bio-distributed in several tissues, infiltrated in tumors and rejected gp350 + tumor cells. Discussion These results support the use of gp350CAR-T cells generated with ZT002 as an Innovative New Drug to treat patients with solid and liquid EBV-associated malignancies.
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高影响因子
FlatChem
·
2022
·
IF 5.829
Shuting Hu,Yuheng Liang,Fuming Chen,Guannan He,Yinzhen Wang,Wei Li,Qinyu He
It is important for organic pollutants to be completely degraded without intermediates. In this work, a novel wide-spectrum-response oxygen-rich double direct Z-scheme heterojunction-structured photocatalyst, g-C 3 N 4 (precursor: urea, CN-U)/g-C 3 N 4 (precursor: thiourea, CN-T)/Bi 2 WO 6 (UTB), was prepared. The ternary system was confirmed by structural analysis and surface and morphological studies. The photocatalytic performances of the synthesized catalysts were estimated by photodegrading rhodamine B (RhB) solution. A more than 92 % mineralization and improved photocatalytic activity were achieved. UTB showed an outstanding photocatalytic performance for RhB degradation compared to CN-T, CN-U, Bi 2 WO 6 , and their binary nanocomposites.The improved photocatalytic properties and n more than 92 % mineralization were attributed to the double direct Z-scheme and oxygen vacancies, which maintained the outstanding redox capability of the electrons, expanded the range of light absorption, and facilitated the separation of photogenerated carriers. This study has demonstrated the prospect in obtaining a double Z-scheme structured photocatalysts with simultaneously high redox potential and narrow E g . This work has also offered a new strategy for fabricating low-consumption and efficient photocatalysts that mineralize more than 92 % of organic pollutants without existing intermediates.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
·
IF 2.694
Kui Wu,Youliang Guan,Zujin Yang,Hongbing Ji
The solid–liquid equilibrium of isophthalonitrile (IPN) in 16 solvents (methanol, ethanol, n-propanol, isopropanol, acetone, ethyl acetate, acetonitrile, chloroform, cyclohexanone, cyclopentanone, methyl acetate, ethyl formate, 2-pentanone, tetrahydrofuran, toluene, and diethyl ether) was measured by using a static equilibrium method at temperatures T = 273.15–324.75 K under atmospheric pressure. The results demonstrated that the solubility of IPN in these 16 monosolvents increased with increasing temperature. The largest solubility values of IPN were found in cyclopentanone, and the lowest were in isopropanol. The values of solubility in ketones were much larger than those in esters and alcohols. In alcohols, the solubility ranked as methanol > ethanol > n-propanol > isopropanol, and the sequence was identical to that of the solvent polarities. The polarity of the solvent is an important factor influencing the solubility profiles of IPN in alcohols, despite that the conclusion is not supported by other kinds of solvents studied. Moreover, the Apelblat equation, λh equation, Wilson model, and nonrandom two-liquid model were used to correlate the experimental values. The calculated values of four models all provided good fitting results with the experimental data, and the values of root-mean-square deviation and relative average deviation (RAD) were no more than 6.84 × 10–4 and 6.84 × 10–3, respectively. Furthermore, the thermodynamic properties of the mixing process for IPN in selected solvents were calculated, that is, mixing Gibbs energy (ΔmixG), molar enthalpy (ΔmixH), and molar entropy (ΔmixS). The results indicated that the mixing process of IPN was a spontaneous and entropy-driven process. The solid–liquid equilibrium data and solution thermodynamics would be helpful for the synthesis and purification of IPN in the industry.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
·
IF 4.539
Qixia Guan,Hao Guo,Ning Wu,Yujuan Cao,Mingyue Wang,Longwen Zhang,Wu Yang
As a new kind of porous organic material, covalent organic framework materials (COFs) have many advantages and have been widely used in catalysis, sensing, drug delivery, gas separation and other fields. In this paper, A COF was successfully prepared based on Schiff base reaction of 1, 3, 6, 8-tetrakis(4-formyl phenyl) pyrene (TFPPy) and 1, 3, 6, 8-tetrakis(4-amino phenyl) pyrene (PyTTA) named as TF-Py COF, and its structure was characterized in detail. By physically combining a pre-prepared three-dimensional carbon material containing nitrogen (3D NCNF-T) and TF-Py COF, followed by deposition of AuNPs, relatively poor conductivity of TF-Py COF was efficiently overcome and a novel electrochemical sensing platform with high sensitivity and high selectivity for simultaneous detection of acetaminophen (ACOP) and 4-aminophenol (4-AP) was successfully constructed. The synergistic effect between TF-Py COF, 3D NCNF-T and AuNPs effectively improves the analytical sensitivity of target analytes. Under optimized experimental conditions, the electrochemical sensing platform has a sensitive volt-ampere response and a wide linear range to both ACOP and 4-AP, and the detection limits are 0.13 μM and 0.05 μM (S/N = 3), respectively. The sensor has been successfully applied to the determination of ACOP and 4-AP in acetaminophen tablets and Yellow River water samples, with high recovery rate, good selectivity and reproducibility, and strong anti-interference ability. It is expected to be widely used in the actual sample detection.
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World Journal of Clinical Cases
·
2021
·
IF 1.337
Shi-Hao Mao,Dan-Dan Feng,Xi Wang,Yi-Hui Zhi,Shu Lei,Xi Xing,Rong-Lin Jiang,Jian-Nong Wu
BACKGROUND Sepsis is a major medical challenge. Magnolol is an active constituent of Houpu that improves tissue function and exerts strong anti-endotoxin and anti-inflammatory effects, but the mechanism by which it reduces intestinal inflammation in sepsis is yet unclear. AIM To assess the protective effect of magnolol on intestinal mucosal epithelial cells in sepsis and elucidate the underlying mechanisms. METHODS Enzyme-linked immunosorbent assay was used to measure tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and regulated on activation, normal T-cell expressed and secreted (RANTES) levels in serum and ileal tissue in animal studies. The histopathological changes of the ileal mucosa in different groups were observed under a microscope. Cell Counting Kit-8 and cell permeability assays were used to determine the concentration of drug-containing serum that did not affect the activity of Caco2 cells but inhibited lipopolysaccharide (LPS)-induced decrease in permeability. Immunofluorescence and Western blot assays were used to detect the levels of RANTES, inhibitor of nuclear factor kappa-B kinase β (IKKβ), phosphorylated IKKβ (p-IKKβ), inhibitor of nuclear factor kappa-B kinase α (IκBα), p65, and p-p65 proteins in different groups in vitro . RESULTS In rats treated with LPS by intravenous tail injection in the presence or absence of magnolol, magnolol inhibited the expression of proinflammatory cytokines, IL-1β, IL-6, and TNF-α in a dose-dependent manner. In addition, magnolol suppressed the production of RANTES in LPS-stimulated sepsis rats. Moreover, in vitro studies suggested that magnolol inhibited the increase of p65 nucleation, thereby markedly downregulating the production of the phosphorylated form of IKKβ in LPS-treated Caco2 cells. Specifically, magnolol inhibited the translocation of the transcription factor nuclear factor-kappa B (NF-κB) from the cytosol into the nucleus and down-regulated the expression level of the chemokine RANTES in LPS-stimulated Caco2 cells. CONCLUSION Magnolol down-regulates RANTES levels by inhibiting the LPS/NF-κB signaling pathways, thereby suppressing IL-1β, IL-6, and TNF-α expression to alleviate the mucosal barrier dysfunction in sepsis.
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INTERNATIONAL JOURNAL OF THERMOPHYSICS
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2021
·Vol. 42
·期 6
·1-24
·
IF 1.608
Liu Ping,Liu Zimin,Zhao Tianxiang,Liu Fei,Liao Quanlan
Densities and viscosities for the binary system of tetraethylene glycol (1) + water (2) at T = (298.15 to 323.15) K were measured as a function of composition under atmospheric pressure. The excess properties, including excess molar volumes ( \(V_{{\text{M}}}^{{\text{E}}}\) ), viscosity deviations (Δ η ), and the excess Gibbs free energy of activation for viscous flow (Δ G * E ) were calculated by using density and viscosity data, respectively. Excess properties indicated that there are hydrogen bonding interactions between tetraethylene glycol and water molecules, which are investigated in detail by FTIR and UV–Vis spectra.
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Genes
·
2020
·Vol. 11
·期 5
·478
·
IF 3.759
Ying Sun,Mei Gao,Seogchan Kang,Chengmin Yang,Hui Meng,Yun Yang,Xiangsheng Zhao,Zhihui Gao,Yanhong Xu,Yue Jin,Xiaohong Zhao,Zheng Zhang,Jianping Han
Dalbergia odorifera, a critically endangered tree species, produces heartwood containing a vast variety of flavonoids. This heartwood, also known as Chinese rosewood, has high economic and medicinal value, but its formation takes several decades. In this study, we showed that discolored wood induced by pruning displays similar color, structure, and flavonoids content to those of natural heartwood, suggesting that wounding is an efficient method for inducing flavonoid production inD. odorifera. Transcriptome analysis was performed to investigate the mechanism underlying wounding-induced flavonoids production inD. odoriferaheartwood. Wounding upregulated the expression of 90 unigenes, which covered 19 gene families of the phenylpropanoid and flavonoid pathways, including PAL, C4H, 4CL, CHS, CHI, 6DCS, F3’5’H, F3H, FMO, GT, PMAT, CHOMT, IFS, HI4’OMT, HID, IOMT, I2’H, IFR, and I3’H. Furthermore, 47 upregulated unigenes were mapped to the biosynthesis pathways for five signal molecules (ET, JA, ABA, ROS, and SA). Exogenous application of these signal molecules resulted in the accumulation of flavonoids in cell suspensions ofD. odorifera, supporting their role in wounding-induced flavonoid production. Insights from this study will help develop new methods for rapidly inducing the formation of heartwood with enhanced medicinal value.Keywords:Dalbergia odorifera;flavonoid biosynthesis;heartwood;mechanical wounding;signals;transcriptome
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高影响因子
危险品化学品经营许可证（带存储）