Analytical Methods
·
2017
·Vol. 9
·期 47
·6669-6674
·
IF 1.9
Hui Huang,Jiao Li,Mengxian Liu,Zizhun Wang,Bingdi Wang,Meini Li,Yongxin Li
A novel multifunctional semiconducting polymer dot (Pdots)/pyrogallic acid (PA) system was successfully established for fluorescence sensing of urea, urease, and pesticides. The Pdots/PA system was pH controllable, emitting strong fluorescence at pH 6.0. However, in an alkaline environment, PA transformed into its quinone structure, which is an efficient fluorescence quencher, resulting in fluorescence quenching of the Pdots/PA system. Furthermore, urea was hydrolyzed in the presence of urease, releasing hydroxyl groups that transformed PA into its quinone structure, resulting in fluorescence quenching of the Pdots/PA system. Therefore, a sensitive detection method for urea and urease was developed. The limits of detection for urea and urease were 0.02 mM and 0.08 U L−1, respectively. Pesticides, such as dimethoate, were able to inhibit the urease activity, resulting in recovery of the fluorescence intensity of the Pdots/PA system. Therefore, the fluorescence turn-on detection of pesticides could be also achieved. A satisfactory result was obtained when using the system to detect pesticides in fruit and vegetable samples.
研究领域:
分析化学
·
生物传感器
·
环境监测
·
食品安全
·
分子探针
·
酶学
✓ 已验证
DOI
高影响因子
Food and Bioprocess Technology
·
2023
·Vol. 17
·期 1
·245-256
·
IF 5.6
Dong Mengxue,Sun Yusha,Xiong Dandan,Song Qi,jia Jie,Liu Xuebo,Sheng Long,Duan Xiang
Wheat gluten protein (WGP) is a fine-quality plant-based protein resource. However, as its unparalleled reticulation structure, the processing properties of WGP are extremely poor, limiting its application. To overcome these drawbacks, the purpose of this work was to embellish wheat gluten protein by three relatively novel and mainstream chemical modifications. The results showed the pH-shifting treatment altered the apparent morphology of protein, showing a uniform flocculent structure, leading to significant improvements in foaming capacity and emulsification property. After deamidation by acetic acid, the solubility of WGP was greatly improved (60.1%), which was nearly four times that of the control group (15.8%), and its foam stability was also significantly improved. The WGP had the highest thermal stability (deformation temperature up to 148 ℃) after TGase deamidation. These results indicate that the three modification methods enhance the functional characters of WGP in different aspects and expand its application potential.
研究领域:
食品科学
·
蛋白质化学
·
化学修饰
·
生物技术
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Ying Fang,KangRui Wang,Qin Li,ChengHong Huang
Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR. EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release. Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL. It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%. Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8. It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
研究领域:
纳米技术
·
药学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Zhijuan Li,Minghao Hou,Baoye Yao,Chuhan Ma,Shitong Zhang,Honglei An,Yichen Liu,Haibao Duan,Tongfei Li,Yawen Tang
The development of high-entropy alloy (HEA) nanocatalysts with ultrasmall size and hollow structures is vital yet challenging for efficient hydrogen evolution reaction (HER) across all pH conditions. Herein, we present a mild and facile hydrothermal strategy for synthesizing hollow PtRuRhCoCu HEA nanospheres (NSs) via self-assembly of sub-3 nm alloy nanoparticles directed by N , N ′-methylenebisacrylamide (MBAA). The resulting PtRuRhCoCu HEA features a multielement composition, interconnected hollow architecture, and well-dispersed ultrafine particles, providing abundant active sites and strong synergistic electronic interactions. As a result, the PtRuRhCoCu HEA catalyst achieves remarkable HER activity with low overpotentials of 10 mV (1 M KOH), 15 mV (0.5 M H 2 SO 4 ) and 30 mV (1 M phosphate-buffered saline, PBS) at 10 mA cm −2 , outperforming commercial Pt black. Moreover, this catalyst also exhibits outstanding long-term stability across a broad pH range, operating continuously for 80 h under alkaline conditions at various current densities without noticeable degradation. The density functional theory (DFT) calculations confirm that this HEA exhibits optimized hydrogen adsorption energy and electronic structure. When integrated into a full electrolyzer, it delivers a cell voltage of 1.48 V at 10 mA cm −2 , demonstrating great potential for water splitting. This work presents an effective design strategy for next-generation HEA electrocatalysts toward green hydrogen production.
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产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2020
·Vol. 78
·期 8
·4551-4568
·
IF 2.014
Li Yinhui,Li Pengwei,Lu Jingqiong,Zhao Yansheng
Stimuli-responsive hydrogels are attracting extensive attention in drug delivery system. Here, a novel pH, thermo and salt sensitivity of composite hydrogels was synthesized in situ from polysuccinimide modified by γ-aminopropyltriethoxysilane with mesoporous molecular sieves (MCM-41) as only crosslinking agent for controlled salicylic acid release. Morphologies and thermal behaviors of KPAsp/MCM-41 hydrogels were characterized by scanning electron microscopy and thermogravimetric analysis. Swelling dynamic behaviors, pH, thermo and salt sensitivities and drug releasing capacities of composite hydrogels were also systematically investigated. The obtained results show that multi-responsive hydrogels are very sensitive to environmental pH, external temperature and ionic valence, which indicated that the MCM-41 addition not only can ameliorate the network structure, but also can enhance the drug loading and releasing capacity. The controlled salicylic acid release rates of MCM-41/KPAsp hydrogels can reach to 33.6% and 61.2% in simulated gastric fluid (pH = 1.2) and in simulated intestinal fluid at (pH = 7.4) at 37 °C, respectively. The as-synthesized multi-responsive hydrogels reported here are promising candidates for application in targeted drug delivery system.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 104
·期 15
·6635-6646
·
IF 3.53
Huang Kai,Zhang Shurong,Guan Xiao,Liu Jing,Li Sen,Song Hongdong
This study aims to use neutral pH optimum arginase as the catalyst for high-efficiency l -ornithine production. Sulfobacillus acidophilus arginase was firstly cloned and overexpressed in Escherichia coli . The purified enzyme was obtained, and the molecular mass determination showed that this arginase was a hexamer. S. acidophilus arginase possessed similarities with the other arginases such as the conserved sequences, purification behavior, and the necessity for Mn 2+ as a cofactor. The maximum enzyme activity was obtained at pH 7.5 and 70 °C. Thermostability and pH stability analysis showed that the arginase was stable at 30–60 °C and pH 7.0–8.5, respectively. The kinetic parameters suggested that S. acidophilus arginase could efficiently hydrolyze l -arginine. Bioconversion with this neutral pH optimum arginase had the advantages of avoiding producing by-product, high molar yield, and high-level production of l -ornithine. When the bioconversion was performed with a fed-batch strategy and a coupled-enzyme system involving S. acidophilus arginase and Jack bean urease, the final production of 2.87 mol/L was obtained with only 1.72 mmol/L l -arginine residue, and the molar yield was 99.9%. The highest production record suggests that S. acidophilus arginase has a great prospect in industrial l -ornithine production.
研究领域:
生物技术
·
酶学
·
微生物学
·
生物化工
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Ting Bai,Dongyan Shao,Jianxin Chen,Yifan Li,Ben Bin Xu,Jie Kong
A drug delivery system that is integrated with fluorescent imaging is an emerging platform for tumor diagnostic and therapy. A pH-responsive fluorescent polymer that can respond to the surrounding medium is a desired component with which to construct an advanced drug delivery system with bioimaging characteristics and controllable drug releasing. In this work, we synthesized novel amphiphilic block copolymers of poly(ethylene glycol)- b -poly(2-(diisopropylamino) ethyl methacrylate- co -dithiomaleimide) (PEG- b -poly(DPA- co -DTM)) and poly(ethylene glycol)- b -poly(2-(dibutylamino) ethyl methacrylate- co -dithiomaleimide) (PEG- b -poly(DBA- co -DTM)) with pH-responsiveness and fluorescence. The block copolymers exhibited relatively stable fluorescence properties in different solvent and excitation-independent fluorescence behaviours. By copolymerizing the responsive segments in the molecule chain, the doxorubicin (DOX)-loaded micelles could be triggered to disassemble, thus releasing DOX at the corresponding pH values and yielding a pH-responsive drug release. Targeted deliveries of the drug within the cell were demonstrated by using the carrier responding to different pH values. The best antitumor effect was obtained by PEG- b -poly(DPA- co -DTM), which immediately released DOX as soon as it entered the tumor cells, as a result of responding to the regional pH level (pH = 6.3). The pH-responsive copolymers showed excellent biocompatibilities, as nearly 85% of cells with these fluorescent micelles survive when the testing concentration goes up to 200 μg mL −1 . In all, these pH-responsive and dithiomaleimide-based fluorescent block copolymers hold great potential in future cancer diagnostic and therapeutic techniques.
研究领域:
高分子化学
·
药剂学
·
生物材料
·
癌症治疗
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS RESEARCH
·
2013
·Vol. 28
·期 10
·1394-1404
·
IF 1.713
Yi Chen,Weijian Xu,Yuanqin Xiong,Chang Peng,Wenyong Liu,Guangsheng Zeng,Yue Peng
The monomer dimethylaminoethyl methacrylate (DMAEMA) was cross-linked by clay Laponite XLG and Laponite XLS, respectively, in situ free-radical polymerization to prepare nanocomposite gels (named as G-NC gels and S-NC gels). From the analysis of yield, transparency, Fourier transform infrared spectroscopy, x-ray, and scanning electron microscopy, it was proved that the clay Laponite XLS is an appropriate cross-linker for preparing PDMAEMA NC gels with better homogeneous and higher yield due to the uniform dispersion of clay in gels. The resulting S-NC gels show obvious temperature and pH double sensitiveness, and the lowest critical solution temperatures of gels with a low clay content are close to the temperature of the human body, which makes this gel a potential candidate for application in drug release. Comparing with the gels cross-linked by a chemical cross-link agent, the S-NC gels exhibit a considerable improvement in the mechanical properties, swelling ratio, and deswelling rate. The properties of S-NC gels could be adjusted by controlling the content of clay.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Mei Shao,Cong Chang,Zuhao Liu,Kai Chen,Yimin Zhou,Guohua Zheng,Zhijun Huang,Haixing Xu,Peihu Xu,Bo Lu
A nanocarrier system of methoxypolyethylene glycol amine (mPEG-NH 2 ) functionalized polydopamine (PDA) coated hollow mesoporous silica nanoparticles (HMSNs-PDA-PEG) was developed with pH-responsive, which combined doxorubicin hydrochloride (DOX) and quercetin (QUR) to reverse multidrug resistance (MDR) and improved anticancer effects on taxol (TAX) and DOX double resistant human colorectal cancer cell line HCT-8 (HCT-8/TAX cells). Well-dispersed nanoparticles (HMSNs-PDA-PEG) were prepared with a dimension of around 170 nm. The surface morphology and chemical properties of HMSNs-PDA-PEG were also successfully characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) method, Fourier transform infrared spectroscopy (FT-IR) and dynamic light scattering (DLS). Drug release experiments results indicated that DOX and QUR (QD) loaded nanoparticles (HMSNs-PDA-PEG@QD) had similar release kinetic profiles of each drug, which all exhibited highly sensitive to pH value due to the surface PDA coating. Additionally, the HCT-8 cells or HCT-8/TAX cells were employed to assess the cellular uptake and cytotoxicity of various drug-free or drug-loaded HMSNs samples. Meanwhile, a series of biological evaluations demonstrated that the HMSNs-PDA-PEG@QD exhibited remarkable ability to overcome MDR compared with free DOX and HMSNs-PDA-PEG@DOX. Taken together, these results revealed that HMSNs-PDA-PEG@QD was suitable as a prospective and efficient drug delivery nanosystem for overcoming multidrug resistance.
研究领域:
纳米技术
·
药学
·
癌症研究
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2020
·
IF 4.845
Yuman Dong,Pengcheng Du,Peng Liu
An intramolecular fluorescence resonance energy transfer (FRET)-based macromolecular theranostic prodrug was designed by directly conjugating Doxorubicin (DOX) as the FRET acceptor onto the naphthalimide side groups in the fluorescent copolymer PPEGMA 20 -PNAP 8 as the FRET energy donor via an acid-labile imine bond, without a fluorogenic linker. The proposed PPEGMA 20 -PNAP 8 -DOX theranostic prodrug showed a high DOX content of 24.3% owing to a conjugation efficiency of > 93% under mild conjugation conditions. It could easily self-assemble into unique theranostic nanoparticles with a D h of 71 nm. The theranostic nanoparticles showed excellent pH-triggered DOX release performance with very low premature drug leakage of 6.3% in normal physiological medium over 129 h, while>91% of the conjugated DOX was released in the acidic tumor intracellular microenvironment. MTT assays indicated the enhanced antitumor efficacy of the proposed theranostic nanoparticles compared with free DOX. Furthermore, because drug release was triggered by pH, orange fluorescence was restored to the blue fluorescence of the backbone copolymer. Such self-tracking pH-responsive colorful fluorescence variations during intracellular drug delivery and release are expected to allow real-time tumor intracellular drug release monitoring and imaging diagnosis.
研究领域:
肿瘤学
·
生物材料
·
药理学
✓ 已验证
DOI
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Liang Dong,Hui Chen,Ting Liu,Junming Zhu,Min Yu,Qingmei Yuan
Three disulfide bond-containing peptide amphiphiles (PA1–3) with different lengths of alkyl tails (PA1 for C6, PA2 for C12, and PA3 for C18) were synthesized by ring-opening polymerization of α-amino acid N-carboxyanhydride followed by post-polymerization modification. The peptide segments were composed of 3-mercaptopropionic acid-modified poly(l-cysteine) [P(Cys-SS-CH2CH2COOH)]. We characterized the chemical structure and molecular parameters by 1H NMR, 13C NMR, gel permeation chromatography, Fourier transform infrared spectroscopy, and circular dichroism spectroscopy. It is found that alkyl-P(Cys-SS-CH2CH2COOH) mainly presents a β-sheet conformation at the solid state. However, the PAs present predominant random coils at pH 7.4 in aqueous solutions. The β-sheet conformation increased dramatically when the concentration of the PA exceeded its critical micelle concentration (ca. 0.3 mg/mL for PA3), indicating the formation of self-assembly-induced β-sheet nanostructures. Elongation of the alkyl chain length or a decrease of the pH of the PA solution can promote the formation of the β-sheet conformation. The three PAs can self-assemble into spherical micelles or nanofibrous hydrogels, which can be utilized as nanocarriers for systemic drug delivery or implants for localized drug delivery, respectively. Cisplatin (CDDP) was loaded as a model medicine to examine the drug delivery potential of PA3. We found that the CDDP-loaded PA3 micelles are stable at pH 7.4, have a spherical morphology with a hydrodynamic diameter of ca. 52 nm, and accomplish pH/reduction dual-responsive release of CDDP. In addition, alkyl-P(Cys-SS-CH2CH2COOH) can self-assemble into nanofibrous hydrogels at pH 5.0–6.0 or upon the addition of certain metal ions and show excellent dynamic reversibility. Moreover, the CDDP-loaded PA3 hydrogel exhibits a sustained release profile and a nearly linear release over 48 h. In vitro cytotoxicity of PA3 also indicates its nontoxicity. Thus, our findings suggest that alkyl-P(Cys-SS-CH2CH2COOH) has great potential for both systemic and localized delivery of therapeutics.
研究领域:
生物材料学
·
药剂学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Energy Materials
·
2022
·
IF 6.959
Haibin Zhong,Guanglei Liu,Yudong Cao,Bin Chen,Mingxin Ye,Jianfeng Shen
Splitting water to produce hydrogen through an efficient and low-cost way requires the development of catalysts based on earth-abundant elements. Among transition metal phosphides, bimetal phosphides demonstrate superior hydrogen evolution reactivity to monometal phosphides. Therefore, we synthesized Ni2P–Co2P nanowire arrays on nickel foam with excellent catalytic performance by hydrothermal and low-temperature phosphorization. In 1 M KOH, the catalyst demonstrates low overpotentials of 99 and 161 mV to reach current densities of 10 and 100 mA cm–2, respectively. In 0.5 M H2SO4, the overpotentials of Ni2P–Co2P are only 94 and 159 mV at current densities of 10 and 100 mA cm–2, respectively. In a neutral solution, the overpotentials of the catalyst are also only 146 and 309 mV at current densities of 10 and 100 mA cm–2, respectively. The Tafel slopes of Ni2P–Co2P are only 53.1, 48.26, and 138.3 mV dec–1 in acid, alkaline, and neutral solutions, respectively. Moreover, the outperformance of its durability was tested for 20 h in acid and alkaline solutions, which maintained a stable current density of 10 mA cm–2. This work demonstrates a feasible route to design transition metal phosphides used in pH-universal HER electrocatalysts.
研究领域:
材料科学
·
电化学
·
可再生能源
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）