JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Jingya Liu,Sidian Zhang,Xiuying Zhao,Yi Lu,Meng Song,Sizhu Wu
Rutin is a natural flavonoid compound with various biological activities, but its low water solubility limits its applications in the food and pharmaceutical fields. Complexation with cyclodextrins (CDs) is a powerful method to improve the poorly solubility of rutin, whereas the mechanism for the inclusion still needs to be explored. This study investigated the characteristic and mechanism for inclusion of rutin with β -cyclodextrin ( β -CD) and 2,6 dimethyl- β -cyclodextrin (DM- β -CD). The molecular simulation provided the possible inclusion structure and revealed that the combination of rutin and CDs is a thermodynamically good process. It also indicated that the complex formed with DM- β -CD had better stability and water solubility compared with β -CD. Then the complexes of rutin with β -CD and DM- β -CD were prepared and characterized by FT-IR, XRD, and DSC analysis. Phase solubility studies demonstrated that the solubility of rutin was significantly enhanced by forming 1:1 stoichiometric inclusion complexes. The effect of DM- β -CD in improving the water solubility of rutin is 146% higher than that of β -CD, which was in accordance with the simulation predictions.
研究领域:
食品科学
·
药学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2022
·
IF 4.132
Haonan Cong,Zihao Ma,Meixi Hu,Junjie Han,Xing Wang,Ying Han,Yao Li,Guangwei Sun
Herein, we developed an efficient and convenient method to address the problem of thickener decomposition in the low- permeability oilfield production process. It is crucial to design breakers that reduce viscosity by delaying thickener decomposition in appropriate environments. By using lignin in biomass as a substrate for β-mannanase immobilization (MIL), we fabricated a gel breaker, surface gelatin-coated β-mannanase-immobilized lignin (Ge@MIL). Through experiments and performance tests, we confirmed that the prepared Ge@MIL can release enzymes at a specific temperature, meanwhile having temperature-sensitive phase change properties and biodegradability. The results also show the tight tuning over the surface coating of Ge@MIL by a water-in-oil emulsion. Therefore, the prepared Ge@MIL has a promising application in the field of oil extraction as a green and efficient temperature-sensitive sustained-release capsule.
研究领域:
石油工程
·
生物技术
·
环境科学
✓ 已验证
DOI
高影响因子
BIOMACROMOLECULES
·
2025
·
IF 5.4
Qianru Li,Yuhe Zhu,Jiahui Ding,Weizhen Pan,Yuwei Hu,Jiao Lu,Sihai Gao,Hongyang Jiang,Zhongyuan Yin,Songwei Tan
Gene therapy is often limited by gene vectors. To reduce the impact of intracellular reactive oxygen species and improve transfection efficiency, thioether bonds were introduced in poly(β-amino ester) (PBAE), a well-known nonviral gene vector, to synthesize PBAE-poly(β-thioether ester) copolymer (PBAE-TE). By varying the monomer feeding ratio and reaction time, the optimized copolymer (PBAE-TE2–5) showed the highest transfection efficiency in SiHa and HeLa cells (75.00 and 78.70%) with a 75:1 copolymer/GFP ratio. PBAE-TE2–5 also demonstrated superior transfection performance compared to PBAE in multiple cell lines (HeLa, Me180, C666–1, and HEK 293T) and better penetration capability in 3D spheroid cell models (SiHa and HEK 293T). The PBAE-TE2–5-CRISPR/Cas9 plasmid polyplexes could effectively edit target genes in HEK 293T (MUC2) and SiHa (HPV16 E7) cells, and inhibit cancer cell proliferation both in vitro and in vivo. These findings highlight the promising potential of PBAE-TE as an effective gene delivery system.
研究领域:
生物材料
·
基因治疗
·
高分子化学
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生物医学工程
·
分子生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zheyi Zhang,Sainan Yu,Jiawei Gu,Feilong Tan,Sibao Wan,Haiyan Gao,Zhen Qin
β-1,3-Glucanases have prospective applications in areas such as functional oligosaccharide preparation, plant protection, and breweries. In this study, a glycoside hydrolase (GH) family 17 β-1,3-glucanase (BbGlc17A) from Bacteroidetes bacterium from a microbial mat metagenome from the Great Salt Lake was identified. BbGlc17A catalyzed the hydrolytic conversion of laminarin into β-glucooligosaccharides with polymerization degrees of 3–8. The optimal catalytic conditions of BbGlc17A were pH 6.5 and 30 °C. In addition to its hydrolytic activity, BbGlc17A also exhibited transglycosidase activities, involving catalysis of the formation of new β-1,6-glycosidic bonds. BbGlc17A exhibits the classic (β/α)8 TIM-barrel structure and possesses an elongated catalytic groove, distinguishing it from other typical β-1,3-glucanases, which promote the forward direction of the transglycoside reaction. This effectively highlights the potential of the enzyme to convert β-1,3-glucan into mixed functional oligosaccharides. These results reveal the catalytic properties and the application potential of the GH family 17 β-1,3-glucanase and provide valuable information about the group of carbohydrate-active enzymes in biochemistry.
研究领域:
生物化学
·
酶学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Biomaterials and Tissue Engineering
·
2023
·Vol. 13
·期 6
·753-764
·
IF 0.1
Cheng Xu-Mei,Ma Rui,Zhou Yan-Bing,Li Tao,Lei Sheng-Nan,Zheng Xin,Zhang Hui-Xiao,Liu Mei-Tian,Zhou Jian-Ye,Li Zhi-Qiang
Bone defect repair is a hot topic in tissue engineering, and the development of economical and effective bone scaffold materials to achieve the goal of repairing bone defects is an important task for researchers. In this experiment, fresh bovine cancellous bone was used as a xenogeneic
bone material. After two high-temperature calcinations, β-tricalcium phosphate (β-TCP) material was obtained. An in-situ co-precipitation method was employed to prepare hydroxyapatite+graphene oxide (HA+GO) composite material. Then, the β-TCP material
was immersed in the HA+GO composite material solution to prepare β-TCP+HA+GO composite material. The surface modification of β-TCP material with HA and GO improved the elastic modulus of the raw material. In vitro cell showed that the composite material was non-toxic,
biocompatible, and promoted the proliferation of MC3T3-E1 cells on the β-TCP+HA+GO material. Finally, it was found that β-TCP+HA+GO composite material effectively stimulated the actin and mineralization behavior of MC3T3-E1 cells, and promoted the expression of bone-related
factors such as Alkaline phosphatase (ALP), Collagen I (COLI), Osteocalcin (OCN), Bone Morphogenetic Protein-2 (BMP-2), and Bone Morphogenetic Protein-7 (BMP-7) in MC3T3-E1 cells In conclusion, β-TCP+HA+GO composite material can be applied in the biomedical field as a bone substitute
or repair material.
研究领域:
组织工程学
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生物医学材料
·
细胞生物学
✓ 已验证
DOI
高影响因子
POLYMER
·
2015
·
IF 3.562
Wan-Xia Wu,Lin Qu,Bei-Yu Liu,Wei-Wei Zhang,Na Wang,Xiao-Qi Yu
A novel linear polyester with acid-labile β -thiopropionate groups was synthesized via the direct lipase-catalyzed polycondensation of methyl 3-((2-hydroxyethyl)thio)propanoate (MHETP) using immobilized lipase B from Candida antarctica (CALB). The influences of reaction conditions such as polymerization temperature and time on the molecular weight of the poly( β -thioether ester) (PTE) were studied. Ring-opening and condensation copolymerization of MHETP with ε -caprolactone (CL) led to the synthesis of novel poly( β -thioether ester- co - ε -caprolactone) copolymers , poly(TE- co -CL), with well-defined composition and narrow polydispersity . The random copolymers were characterized by 1 H NMR, 13 C NMR, 2D NMR , GPC , TGA and DSC . The degradation of the poly( β -thioether ester) under acidic conditions was investigated by GPC and 1 H NMR spectroscopy.
研究领域:
生物化学
·
材料科学
·
合成生物学
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2020
·Vol. 10
·期 3
·302
·
IF 3.52
Meng Guo,Zhen Wei,Jianming Yang,Zunyuan Xie,Weiqiang Zhang
In the palladium-catalyzed C−C coupling reaction, electron-rich phosphine ligands and a catalytic amount of catalyst loading are required in most cases. Herein, a bench-stable, easily modified and less toxic alkynone was utilized in palladium-catalyzed Sonogashira coupling to replace conventional phosphine ligands. With 1-(4-methoxyphenyl)-3-phenyl-2-yn-1-one (L2) as the ligand, catalyst loading was reduced to 5-10 ppm. In this newly developed catalytic system, a variety of (hetero)arene iodines and alkynes could be tolerated, resulting in good yields of the corresponding cross-coupling products.Keywords:α;β-alkynone;Pd-catalyzed C−C coupling;π-acidicity
研究领域:
有机化学
·
催化化学
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Jing Qi,Jichang Tu,Xian Xie,Hongling Wang,Ruiqi Xie
In view of the combination of dodecylamine and hydroxamate can improve the flotation recovery of spodumene, a novel β -amino-hydroxamate surfactant, i.e., N -(3-(dodecylamino)) hydroxamic acid (DAHA), was designed and introduced into spodumene flotation. The micro-flotation performance indicated that DAHA performed excellent collecting ability toward spodumene, and 2.0 × 10 -4 mol/L of DAHA floated out about 90.2% of spodumene, while that of sodium oleate (NaOL) was only about 57.4%. The zeta potential of DAHA-treated spodumene revealed the effective chemisorption of hydroxamate group on spodumene surface. FTIR and XPS recommended that the hydroxamate group in DAHA chelated with surface Al sites to form five-membered hydroxamate-(O,O)-Al complexes. The affinity of DAHA on spodumene spontaneously enhanced by the protonated amino group (–NH 3 + ), which facilitated the chelation of hydroxamate with the surface Al atoms and strengthened the hydrophobicity of spodumene.
研究领域:
矿物加工工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Jingwen Zhang,Binge Zhang,Feiyu Duan,Zehui Xuan,Tong Sun,Lili Lu
β-Galactosidases are important enzymatic tools for glycosylation, but their properties vary greatly with the source. Here, ten putative β-galactosidase genes, designated as bga 1 to bga 10, encoding proteins Bga1 to Bga10, were mined from an environmental metagenomic dataset comprising 119,152 sequences. Five of the encoded enzyme proteins exhibited less than 80% sequence similarity to known enzymes, but displayed conserved catalytic sites in their predicted three-dimensional models. After heterologous expression and characterization, two recombinant enzymes showed specific hydrolysis activity toward o -nitrophenyl-β- d -galactopyranoside. One of them, Bga4R, exhibited remarkable activity at pH 7.4 and 50℃, with excellent alkaline stability. Notably, Bga4R tolerated a wide range of acceptors for transglycosylation. It catalyzed galactosyl transfer to various monosaccharides and sugar alcohols, and enabling the synthesis of diverse glycosylated derivatives. This study identifies a novel GH 1 β-galactosidase as a powerful tool for glycosylation engineering, with promising potential for synthesizing galactosides valuable to food and pharmaceutical industries.
研究领域:
生物化学
·
微生物学
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2020
·
IF 4.335
Xiao-Yu Zhan,Hua Zhang,Yu Dong,Jian Yang,Shuai He,Zhi-Chuan Shi,Lei Tang,Ji-Yu Wang
The B(C6F5)3-catalyzed chemoselective hydrosilylation of α,β- and α,β,γ,δ-unsaturated ketones into the corresponding non-symmetric ketones in mild reaction conditions is developed. Nearly 55 substrates including those bearing reducible functional groups such as alkynyl, alkenyl, cyano, and aromatic heterocycles are chemoselectively hydrosilylated in good to excellent yields. Isotope-labeling studies revealed that hexafluoro-2-propanol also served as a hydrogen source in the process.
研究领域:
有机化学
·
催化化学
·
合成化学
✓ 已验证
DOI
高影响因子
Journal of Building Engineering
·
2024
·
IF 6.7
Jinglong Huang,Yilian Li,Yunhua Zhang
In order to improve the carbonation properties of steel slag, the carbonation reaction process of steel slag and the formation of carbonation products were regulated by the interaction between multiple hydroxyl groups within β-cyclodextrin (β-CD) molecule and calcium ions leached from steel slag. Through a meticulous experimental design, varying contents of β-CD, from 0.0 % to 5.0 %, were mixed with steel slag to investigate their impact on carbonation kinetics, mechanical strength, and microstructural evolution. The results indicate that β-CD significantly accelerates the carbonation process of steel slag, with a particular boost to the carbonation of β-dicalcium silicate (β-C 2 S), a key mineral contributing to the carbonation activity. At an optimal β-CD concentration of 2.5 %, the compressive strength of the treated slag reached a peak of 138.5 MPa, marking a 13.4 % improvement over the control sample. Similarly, the CO 2 uptake was optimized at 17.9 %, increasing by 21.8 %. Mechanism analysis reveals that β-CD not only facilitates carbonation but also refines the resulting carbonation products, leading to a denser microstructure and enhanced mechanical properties. However, at a β-CD content of 5.0 %, the carbonation process was hindered by excessive viscosity. These pivotal findings propose an innovative strategy to augment the efficiency of carbonation and the inherent material properties of steel slag, thereby fostering a more sustainable approach to the utilization of industrial byproducts.
研究领域:
材料科学
·
环境工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Applied Sciences-Basel
·
2018
·Vol. 8
·期 9
·1481
·
IF 1.689
Yao Ren,Haoyu Xie,Li Liu,Dongying Jia,Kai Yao,Yuanlong Chi
β-glucan extract (GE) was obtained from highland barley bran using alkaline–acid–alcohol extraction method. The stability, solubility, foaming ability, and prebiotics characteristics of GE were assessed consecutively. GE demonstrated excellent heat stability (hardly degraded at 220 °C) and pH stability, especially at neutral or alkaline condition, and its solubility was significantly influenced by temperature instead of pH or NaCl, achieving 0.91 g/100 g at 100 °C. Good foaming ability and foam stability of GE were observed during low temperatures (≤40 °C), neutral or alkaline condition. GE indicated a strong anti-digestibility capacity of resisting the hydrolysis of α-amylase and simulated human gastric acid. Interestingly, GE could effectively promote the growth ofLactobacillus bulgaricusandBifidobacterium adolescentis, which was close to fructooligosaccharide. The results of this study could offer valuable information for the application of β-glucan from highland barley as prebiotics in promoting human intestinal health metabolism.Keywords:β-glucan;highland barley;processing characteristics;prebiotics
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）