INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhongyu Shi,Xiasen Wei,Yunfan Wei,Zheyi Zhang,Sibao Wan,Haiyan Gao,Zhen Qin
A novel glycoside hydrolase (GH) family 16 multi-domain β-1,3-1,4-glucanase (FsGlc16A) from Fibrobacter sp. UWP2 was identified, heterogeneously expressed, and its enzymatic properties, protein structure and application potential were characterized. Enzymological characterization showed that FsGlc16A performed the optimal catalytic activity at pH 4.5 and 50 °C with a specific activity of 3263 U/mg. FsGlc16A exhibited the substrate specificity towards oat β-glucan, barley β-glucan and lichenan, and in addition, it hydrolyzed oat β-glucan and lichenan into different β-glucooligosaccharides with polymerization degrees of 3–4, which further illustrated that it belonged to the endo-type β-1,3-1,4-glucanase. FsGlc16A was classified in subfamily25 of GH16. A ‘PXSSSS’ repeats domain was identified at the C-terminus of FsGlc16A, which was distinct from the typical GH family 16 β-1,3-1,4-glucanases. Removing the ‘PXSSSS’ repeats domain affected the binding of the substrate to FsGlc16A and reduced the enzyme activity. FsGlc16A displayed good potential for the applications, which hydrolyzed oat bran into β-glucooligosaccharides, and reduced filtration time (18.89 %) and viscosity (3.64 %) in the saccharification process. This study investigated the enzymatic properties and domain function of FsGlc16A, providing new ideas and insights into the study of β-1,3-1,4-glucanase.
研究领域:
生物化学
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酶学
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微生物学
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食品科学
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PubMed
高影响因子
TEXTILE RESEARCH JOURNAL
·
2017
·
IF 1.443
Ruijun Wang,Zhengchu Liu,Lifeng Cheng,Shengwen Duan,Xiangyuan Feng,Ke Zheng,Yi Cheng,Jie Zeng
Xylanase plays an important role in the hydrolysis of hemicellulose and has gained much attention in the field of biological degumming. The research for xylanases with cellulase-free and high activity for biological degumming has intensified in recent years. In the present research, heterologous expression of a novel endo-β-1,4-xylanase (GH30) from Dickeya dadantii DCE-01 in Escherichia coli BL21 (DE3) was reported. Biochemical characterization of the enzyme and a potential application in ramie biological degumming was discussed. The results showed that the xylanase gene consists of 1251 nucleotides, belonging to glycoside hydrolase family 30 (GH30). The optimal activity of the xylanase was observed at 50℃ and a pH value of 6.4. The Km and Vmax values for beechwood xylan were 14.25 mg/mL and 296.6 μmol/mg, respectively. The catalytic activity was enhanced by addition of 1 mM Cu2+, Ca2+, Mg2+, and K+. The recombinant enzyme was specific for xylan substrates. The enzyme exhibited hydrolytic activity toward ramie hemicellulose. The recombinant xylanase could be effectively applied to ramie degumming.
研究领域:
生物化学
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生物技术
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酶学
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纺织科学
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高影响因子
JOURNAL OF PLANT PATHOLOGY
·
2021
·Vol. 103
·期 2
·549-561
·
IF 1.729
Win Theint Theint,Bo Bo,Malec Przemyslaw,Khan Sikandar,Fu Pengcheng
Trichoderma sp. was isolated from the rhizosphere soil of a healthy banana plant grown in a Panama disease-contaminated field (suppressive soil). The selected strain, identified as Trichoderma asperellum , exhibited antifungal activity against four species of phytopathogenic fungi from the Fusarium genus ( F. oxysporum, F. fujikuroi, F. tricinctum, F. cantenulatum ). Antagonism related mechanisms associated with the T. asperellum isolate against four fungal phytopathogens were studied in vitro both indirectly by the observation of growth competition and directly by antibiosis and mycoparasitism . It was shown that T. asperellum could suppress growth of phytopathogens in the range of 65–74%, as well as inhibits spore germination by 30- 75%. Moreover, T. asperellum was demonstrated to secrete mycolytic enzymes (chitinase and β-1,3, glucanase), which are potentially able to degrade cell walls of the phytopathogens. The observed accumulation of respective transcripts and enzymatic activities of both chitinase and β-1,3, glucanase were remarkably higher in pathogen-induced cultures. The reduction of disease severity by 70% was observed in pot cultures of banana plantlets infected previously with F. oxysporum after 9 weeks of treatment with T. asperellum. Our findings demonstrate that T. asperellum as originated from banana wilt-contaminated plantations express anti-pathogenic effects based on various modes of action, including competitiveness, antibiosis, and mycoparasitism and might be potentially used as a biological control agent to cope with diseases caused by a broad spectrum of pathogenic fungi of Fusarium genus.
研究领域:
植物病理学
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微生物学
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生物防治
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DOI
高影响因子
Chemical and Biological Technologies in Agriculture
·
2025
·Vol. 12
·期 1
·1-22
·
IF 5.2
Wu Jiahui,Yuan Yonglei,Qu Liping
As an edible algae with rich nutrients and high medicinal value, Durvillaea antarctica has certain potential in anti-aging, but there is currently limited research on the separation, purification and activity of its anti-aging active ingredients. We successfully extracted and purified a new β-(1,3)-D-glucan with a special branched structure from Durvillaea antarctica using subcritical water extraction combined with ultrafiltration membrane (SWE-UFM). The polysaccharide, named DAG-1, consists of glucose (92.70%) and has a weight-average molecular weight of 10149 Da. The GC–MS and 1D/2D NMR confirmed that DAG-1 was composed of 3)-β-D-Glcp-(1 → , → 3,6)-β-D-Glcp-(1 → , β-D-Glcp-(1 → and → 6)-α-D-Glcp-(1 → , and β-glycosidic linkages between these sugar units. Animal experiments showed that DAG-1 has significant anti-skin aging effects by inhibiting the reduction of superoxide dismutase (SOD) activity, accumulation of malonic dialdehyde (MDA), and decline in hydroxyproline (HYP) content in collagen fibers of D-gal-induced mouse skin. To further elucidate the mechanism of DAG-1, we found that DAG-1 can alleviate senescence in human dermal fibroblasts (HDFa), inhibit degranulation of human cutaneous mast cells (HCMC), and modulate inflammatory factors (TNF-α, IL-6) production. Additionally, DAG-1 may bind to c-KIT receptors and downregulate the expression of KITLG/KIT, regulating the growth and activation of mast cells, and thus alleviate skin aging. These findings suggest that DAG-1 is a potential anti-aging ingredient for functional food, cosmetic, and medicine development.
研究领域:
海洋生物学
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药理学
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功能性化妆品
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生物化学
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 5
·1751-1764
·
IF 5
Hamouda Hamed I.,Fan Yi-Xuan,Abdalla Mohnad,Su Hang,Lu Ming,Li Fu-Li
Abstract Lam16A is a novel GH16 β-1,3–1,4-lichenase isolated from the genus Caldicellulosiruptor which can utilize untreated carbohydrate components of plant cell walls. Its catalytic module has been characterized that the six carbohydrate-binding modules (CBMs) were queued in the C-terminus, but their roles were still unclear. Here, full-length and CBM-truncated mutants of Lam16A were purified and characterized through heterologous expression in Escherichia coli . The profiles of these proteins, including the enzyme activity, degrading efficiency, substrate-binding affinity, and thermostability, were explored. Full-length Lam16A with six CBMs showed excellent thermostability and the highest activity against barley β-glucan and laminarin with optimum pH of 6.5. The CBMs stimulated degrading ability of the catalytic module, especially against β-1,3(4)-glucan-based polysaccharides. The released products from β-1,3–1,4-glucan by Lam16A or its truncated mutants revealed an endo-type glycoside hydrolase. Lam16As exhibited strong binding affinities to the insoluble polysaccharides, especially Lam16A-1CBM. The degradation of yeast cell walls by Lam16A enzyme solution relative to the control reduced the absorbance values at OD 800 by ~ 85% ± 1.2, enabling the release of up to ~ 0.057 ± 0.0039 µg/mL of the cytoplasmic protein into the supernatant, lowering the viability of the cells by ~ 70.3% ± 6.9, thus causing significant damage in the cell wall structure. Taken together, CBMs could influence the substrate specificity, thermal stability, and binding affinity of β-1,3–1,4-glucanase. These results demonstrate the great potential of these enzymes to promote the bioavailability of β-1,3-glucan oligosaccharides for health benefits.
Key points
Carbohydrate-binding modules strongly influenced the enzyme activity and binding affinity, and further impacted glycoside hydrolase activity.
Lam16A enzymes have sufficient ability to hydrolyze β-1,3–1,4-glucan-based polysaccharides.
Lam16As provide a powerful tool to promote the bioavailability of β-1,3-glucan oligosaccharides.
研究领域:
生物化学
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微生物学
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酶学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2021
·Vol. 109
·期 12
·2506-2515
·
IF 4.396
Jing Wei Jin,Min Zhi Rong,Ming Qiu Zhang,Wing-Leung Wong
β-1,3-D-glucan has been reported to have a series of bioactivities including antitumor, antimicrobial, anti-inflammatory and antioxidative effects; however, its insolubility in neutral aqueous solution significantly restricts the potential application in biological and medicine fields. Herein, a water-soluble aminated β-1,3-D-glucan (AG) was synthesized and the anti-inflammatory effect, cytotoxicity and plasmid DNA (pDNA) binding capacity of AG, serum stability, the particle sizes and zeta potentials of AG/pDNA nanocomposites, and the transfection efficiency and mechanism of action were studied. AG shows no obvious cytotoxicity within the range of working concentration (1–64 μg/ml) and it exerts potent anti-inflammatory effect independent on Dectin-1 and TLR2. AG/pDNA nanocomposites prepared by electrostatic interaction possess an appropriate particle size ranged from 192.8 to 118.4 nm and zeta potentials ranged from 20.880 to 27.16 mV with the N/P ratios from 5 to 100. AG/pDNA nanocomposites at the N/P ratios of 10 and 20 were able to show superior transfection efficiencies in RAW 264.7 cells as a result of their suitable particle size, zeta potential, anti-inflammatory effect, and the specific interaction with pattern recognition receptors (Dectin-1 and TLR2). These results indicate that AG is a potential candidate for DNA delivery system due to its potent anti-inflammatory effect and high transfection efficiency.
研究领域:
药学
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生物技术
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免疫学
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分子生物学
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DOI
PubMed
高影响因子
CELLULOSE
·
2015
·Vol. 22
·期 1
·329-338
·
IF 3.573
Yu Yeli,Zhang Yong,Yang Xiaogang,Liu Hongyi,Shao Lan,Zhang Xiumei,Yao Juming
A cellulose-based superabsorbent exhibits excellent water absorbency and retention performance as well as biodegradability. However, the detailed biodegradation process in soil has not been monitored yet. Yellowing is another important factor affecting its appearance and storage. In this work, cellulose from flax yarn wastes (FYW) was used as the skeleton material to synthesize a cellulose-g-P(AA-co-AM) (FYW/PAA) superabsorbent. The properties of the FYW/PAA superabsorbent were characterized, especially its biodegradation process and yellowing mechanism. The resultant FYW/PAA presented excellent water absorbency and retention capabilities of 886 g/g in deionized water and 73.2 wt% after being dried at 60 °C for 12 h, respectively. The fracturing of β-1,4-glycosidic bonds on the flax-cellulose macromolecules is the main reason for the biodegradation of FYW/PAA in soil. This causes a 70.8 wt% degradation after FYW/PAA has been buried in soil for only 60 days. The drying method and UV irradiation have important implications for the yellowing of FYW/PAA. They facilitate the generation of chromophore and auxochrome groups on the flax-cellulose macromolecules. 2,6-Di-tert-butyl-4-methylphenol (BHT) exhibits excellent antioxidant properties to inhibit the yellowing of FYW/PAA products.
研究领域:
环境科学
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材料科学
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化学工程
·
高分子科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yanli Wang,Siwei Zhong,Ke Yang,Ruifeng Luo,Linxin Dai,Wenzhen Zhong,Yan Ye,Chaomei Fu,Dasheng Lin,Nan Li,Jianping Chen,Chuan Zheng,Shu Fu,Fei Gao
Herein, a β-1,3- D -glucan based yeast cell wall loaded with co-loaded nanoparticles of Rhein (RH) and Emodin (EMO), was developed for the combined treatment of ulcerative colitis (UC) by modulating gut microbiota and the Th17/Treg cell balance. This was achieved through an oral “nano-in-micro” advanced drug delivery system. Specifically, RH was grafted onto the HA chain via disulfide bonds to synthesize a reduction-sensitive carrier material and then used to encapsulate EMO to form nanoparticles with a specific drug ratio (denoted as HA-RH/EMO NPs). As anticipated, HA-RH/EMO NPs were encased within the “nests”-yeast cell wall microparticles (YPs), efficiently reach the colon and then released gradually, this occurs mainly due to the degradation of β-1,3- D -glucan by β-glucanase. Additionally, HA-RH/EMO NPs demonstrated a significant reduction-sensitive effect in GSH stimulation evaluations and a remarkable ability to target macrophages in in vitro cell uptake studies. Notably, HA-RH/EMO NYPs reduced inflammatory responses by inhibiting the PI3K/Akt signaling pathway. Even more crucially, the oral delivery and drug combination methods significantly enhanced the regulatory effects of HA-RH/EMO NYPs on gut microbiota and the Th17/Treg balance. Overall, this research marks the first use of YPs to encapsulate two components, RH and EMO, presenting a promising therapeutic strategy for UC.
研究领域:
药学
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免疫学
·
胃肠病学
·
纳米技术
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微生物学
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DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 12
·6021-6036
·
IF 3.094
Zhang Xin,Wang Yibing,Liu Jiaqi,Wang Weiyang,Yan Xuecui,Zhou Yifa,Cui Jing,Yuan Ye
Because β-1,6-galactans are significant components in arabinogalactans from plant cell walls, identifying selective endo-β-1,6-galactanases is crucial to degrading these polysaccharides and to analyzing and modifying their structures. Here, we cloned and expressed in E. coli a novel endo-β-1,6-galactanase in the glycosidic hydrolase family 30 (GH30) from Penicillium oxalicum . Our recombinant PoGal30 hydrolase (1464 bp gene) that contains an N-terminal His-tag for purification by nickel affinity chromatography has a specific activity of 3.8 U/mg on the substrate de-arabinosylated gum Arabic (dGA) polysaccharide. The enzyme has 487 residues with a molecular mass of 60 kDa, an isoelectric point of 6, and functional pH and temperature optima of pH 2.5 to pH 5.0 and 40 °C, respectively. While the activity of PoGal30 is activated by Mg 2+ (5 or 50 mmol/L), it is completely inhibited by Cu 2+ and Fe 3+ (50 mmol/L) and partially inhibited by Hg 2+ , EDTA, and SDS (50 mmol/L). The enzyme demonstrates high specificity towards β-1,6-galactosidic linkages in dGA, but is inactive against aryl-glycosides and galactobioses with different linkages. Using PoGal30 is, therefore, an effective approach to analyzing the fine structure of polysaccharides and preparing bioactive oligosaccharides.
研究领域:
生物化学
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分子生物学
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酶学
·
植物细胞壁分析
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chuying Huo,Xueting Zhang,Quanwei Zhang,Jing Li,Wenting Zhao,Simin Qi,Hauyee Fung,Wei Yang,Xi Shi,Huiyuan Cheng,Hubiao Chen,Joshua Kashun Ko,Karl Wahkeung Tsim,Hiuyee Kwan,Lifeng Li,Quanbin Han
While Dendrobii β-1,4-mannan (DOP) shows promise in treating colitis, previous research only correlated changes in gut microbiota composition with DOP's effects, without elucidating the specific mechanism by which gut microbiota mediates DOP's bioactivities. This study addresses this crucial gap by demonstrating the essential role of vancomycin-sensitive gut bacteria in mediating the anti-colitis effects of DOP. Using targeted antibiotic perturbation, we found that vancomycin, but not neomycin, abolished both DOP degradation and its therapeutic effects in colitis mice. This loss of efficacy was attributed to the depletion of mannan endo-1,4-β-mannosidase (EC 3.2.1.78), a verified enzyme for DOP catabolism. The expression of this enzyme was increased in DOP-treated microbiota, together with a stronger catalytic ability in DOP degradation. We further identified Bifidobacterium pseudolongum , a vancomycin-sensitive species, as an important contributor to DOP fermentation and its anti-colitis effects. This bacterium initiates DOP's degradation by cleaving it into bioactive mannan oligosaccharides. In summary, this research reveals that the breakdown of DOP by mannan endo-1,4-β-mannosidase in vancomycin-sensitive B. pseudolongum plays a significant role in its anti-colitis effects. This work advances the field by demonstrating that vancomycin-sensitive bacteria, such as B. pseudolongum , mediate DOP's efficacy and establishing polysaccharide catabolism as critical for its therapeutic activity.
研究领域:
微生物学
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胃肠病学
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免疫学
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分子生物学
·
营养科学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yuejie Qiu,Longbin Li,Leping Zhang,Jianxin Jiang,Liwei Zhu
This study develops a catalytic system using pyruvic acid (PYA) and Fe2+ to efficiently coproduce xylo-oligosaccharides (XOS) and (manno-oligosaccharides) MOS from food material (Gleditsia sinensis Lam. fruit.) and its waste peel, respectively. The system achieved maximum yields of 67.12% XOS and 57.32% MOS without toxic byproducts such as furfural and 5-hydroxymethylfurfural (5-HMF) below 130 °C. The process employs a two-stage mechanism, where PYA cleaves galactose side chains and xylan backbones, while Fe2+ stabilizes β-1,4-glycosidic bonds, resulting in polymerization degree ratios of X2–3/X4–5 at 0.975 and M2–3/M4–5 at 0.96. The composite oligosaccharides, PFMX, showed significant prebiotic effects, enhancing Bifidobacterium adolescentis growth to 3.4 × 108 CFU/mL─144% higher than commercial fructooligosaccharides (FOS). The concentration of short-chain fatty acids (SCFAs) was 15.09 g/L, with specific products propionic acid (1.44 g/L) and butyric acid (0.15 g/L). PFMX effectively regulates substrate utilization and SCFA metabolism, offering a promising strategy for developing functional foods that target gut inflammation.
研究领域:
催化化学
·
碳水化合物化学
·
食品科学与技术
·
益生元与肠道微生物
·
绿色化学
·
生物炼制
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PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2018
·Vol. 29
·期 9
·1-11
·
IF 2.448
Hong Lei,Shen Meiting,Fang Jiaxi,Wang Yezhao,Bao Zhiyuan,Bu Shizhong,Zhu Yabin
In this work, two kinds of hyaluronic acid (HA)-based hydrogels were fabricated: one is made from physical freezing-thawing of HA solution (HA1), and the other is from chemical cross-linking of HA and polysaccharide (HA2). They were applied to repair full-thickness skin defects with New Zealand rabbits as the test animals, using powder HA and cotton dress as the references. The wound starts to heal after wounds were disinfected with iodine followed by coating with HA2, HA1, HA and cotton dress (the control), respectively. They were recorded as 4 treatments (groups), HA2, HA1, HA and the control. The healing progress was followed and tested in the duration of 56 days, and the biological repairing mechanism was explored. From the wound area alteration, white blood cell (WBC) measurements and H&E staining, HA2 was the most promising treatment in promoting the wound healing with least serious scar formation. Immunochemistry analyses and real-time PCR tests of the bio-factors involved in the wound healing, vascular endothelial growth factor (VEGF), alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1 (TGF-β1), exhibited that HA2 enhanced VEGF and α-SMA secretion but reduced TGF-β1 expression at early stage, which alleviated the wound inflammation, improved the skin regeneration and relieved the scar formation.
研究领域:
生物材料
·
伤口愈合
·
组织工程
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）