FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Jilong Lu,Meizhen Xie,Lipin Chen,Yong-Huan Yun
Colorimetric indicators of real-time food spoilage detection are typically fabricated using chromogenic agents or nanoparticles; however, their potential migration into food raises considerable safety concerns. Although structurally colored functional biomaterials, which do not pose such risks, represent promising alternatives, their application in food spoilage detection remains largely unexplored. In this study, a structurally colored chitosan membrane (CSM) was obtained from shrimp shells, and its responsiveness to acids and aldehydes was investigated. Fresh coconut water was used to evaluate the performance of CSM as a colorimetric spoilage indicator. Results indicate that CSM is a mesoporous material with a Bouligand structure and a deacetylation degree of 83.93 %. The specific surface area, average pore volume, and average pore size of CSM were 0.44 m 2 /g, 0.001 cm 3 /g, and 9.67 nm, respectively. CSM exhibited chromatic responsiveness to acids at a low pH of 3 due to the protonation of –NH 2 groups, as well as to aldehydes via the formation of imine bond (C=N). Acid and aldehyde exposure altered the crystalline polymorphism of chitosan in CSM. After 18 h of storage at room temperature, the membrane successfully detected spoilage in fresh coconut water through visible color changes, which can be attributed to the synergistic effects of organic acids and aldehydes accumulated during spoilage. These findings demonstrate the effectiveness of CSMs in detecting food spoilage. To our knowledge, this study is the first to demonstrate the potential application of structurally colored CSMs for detecting food spoilage, offering a novel approach for developing food spoilage indicators.
✓ 已验证
产品关联
DOI
高影响因子
Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-23
·
IF 5.7
Xu Jin-Di,Gao Xiao-Qin,Zhong Rong-Ling,Zhou Jing,Wang Ting,Chen Can,Yao Wei-Feng,Geng Ting,Zhang Yi,Li Song-Lin,Zhang Li
Background Shi–Zao–Tang (SZT), a classical formula of Traditional Chinese Medicine orally used for treating malignant ascites effusion (MAE), is made by mixing the powder of Kansui Radix (KR), Euphorbiae Pekinensis Radix (EPR) and fried Genkwa Flos with the decoction of Jujubae Fructus. According to Chinese Pharmacopoeia, vinegar-processed KR and EPR should be used in oral administration. However, toxicity and efficacy of SZT containing vinegar-processed KR and EPR (VSZT) versus SZT in MAE rats, and the potential mechanisms of VSZT against MAE, remain unknown. Here, we comparatively studied the quality, toxicity and efficacy of SZT and VSZT, and explored the potential mechanisms of VSZT against MAE. Methods Main components in SZT and VSZT were quantified by liquid chromatographic coupled with triple quadrupole tandem mass spectrometry. The intestinal toxicity and efficacy of SZT and VSZT were comparatively investigated in MAE rats. Specially, intestinal toxicity was evaluated by intestinal barrier function, histopathology and oxidative damage. The efficacy was investigated by amount of ascites, indices in excretion, intestinal motility and inflammation. The potential mechanisms of VSZT treats MAE were explored through integration of metabolomics, 16S rRNA and Western blotting analysis. Results VSZT contains less 3- O - EZ and more ingenol than SZT. VSZT showed reduced intestinal toxicity than SZT in MAE rats. Both SZT and VSZT indiscriminately decreased the amount of ascites and ascitic inflammatory cytokines, promoted urination and defecation, increased fecal water content and intestinal motility. VSZT reversed endogenous metabolism and gut microbiota disorders, down-regulated colonic cAMP, PKA, p-CREB/CREB and AQP3, as well as mesenteric p-VEGFR2/VEGFR2, p-SRC/SRC, and p-VE-cadherin/ VE-cadherin in MAE rats. Conclusion VSZT preserved the efficacy of SZT on MAE with lower intestinal toxicity. VSZT increased water excretion and decreased MAE formation to alleviate MAE through regulating gut microbiota, restoring tryptophan and tyrosine metabolism disorders, and affecting cAMP-PKA-CREB-AQP3 and VEGFA-VEGFR2-SRC-VE-cadherine pathway.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2025
·
IF 3.2
Chen Yang,Yang Jian-xia,Yv Tian,Zhang Xin-rui,Gong Gui-zhen,Zhu Yuan-ting,Yao Jin,Wu Yi,Zhang Yv-han
AimsThis study aimed to investigate the capacity of lactic acid bacteria (LAB) isolated from yak calves to metabolize xylo-oligosaccharides (XOS) and to identify potential probiotic strains that could enhance the gut health of yak calves.Methods and ResultsLAB isolates were recovered from fifteen fecal samples collected from yak calves. Their capacity to utilize XOS as the sole carbon source was evaluated, and their metabolic characteristics, molecular mechanisms, and functional properties associated with XOS metabolism were examined through integrated phenotypic and genomic analyses. Consequently, forty-one LAB isolates were identified from ten of the fifteen yak calves and tested for XOS utilization. Among these, 51.22% (21/41) exhibited species- and strain-specific XOS utilization, including Limosilactobacillus mucosae (10 isolates), Lactobacillus brevis (10 isolates), and Lactiplantibacillus plantarum (1 isolates). L. mucosae YT200 showed the highest acid production (pH 4.50) and short-chain fatty acid accumulation, particularly acetic acid (745.00 ± 16.24 mM). Genomic analysis revealed three gene clusters encoding GH43 α-L-arabinofuranosidase, GH51 α-L-arabinofuranosidase, and GH43 β-xylosidase, enabling the hydrolysis of arabinosyl-substituted and linear XOS. Strain YT200 also demonstrated antagonism against common gut pathogens including Escherichia coli and Staphylococcus aureus, sensitivity to seven antibiotics, and no transferable resistance genes.ConclusionThis study reveals the mechanistic basis of XOS metabolism in L. mucosae YT200 and identifies L. mucosae YT200 as a promising candidate for synbiotic development.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Chao Yu,Zongshuo Li,Ying Wang,Yanxia Shu,Zhenghui Liu,Weidong Li
Cyclophosphamide (CTX) is a common anticancer drug that can cause immunosuppression and disrupt the intestinal microbiota when taken long-term or at high doses. Lonicerae Japonicae Flos polysaccharides (LJFP), identified as the principal bioactive constituents of L. Japonicae Flos, have emerged as promising immunomodulatory agents. Herein, we explored the molecular mechanisms through which LJFP alleviate CTX-induced immunosuppression in mice. As determined via gas chromatography (GC), LJFP contain arabinose, rhamnose, ribose, xylose, mannose, fructose, galactose, and glucose. LJFP mitigated the decline in body weight and immune organ indexes in CTX-induced mice, while promoting cytokine production. Moreover, LJFP stimulated immunity by reshaping the gut microbiota of mice, where Muribaculaceae and Alistipes played key roles. LJFP also increased short-chain fatty acid (SCFA) levels in the mouse colon and feces. Network pharmacology analyses identified 31 core targets and relevant signaling pathways implicated in the treatment of immunosuppression. Molecular docking indicated strong binding of the eight monosaccharide components to key targets EGFR, HSP90AA1, and IL2. Notably, fifteen of these targets specific to the gut further suggested that the mechanism through which LJFP ameliorates immunosuppression is intricately linked to the gut microbiota. The current findings highlight the potential of LJFP as a functional food or alternative therapeutic against immunosuppression.
✓ 已验证
产品关联
DOI
高影响因子
POULTRY SCIENCE
·
2024
·
IF 3.8
Xiaoying Liu,Kailong Qin,Chaohui Wang,Xi Sun,Yun Li,Yanli Liu,Xiaojun Yang
The hyperplasia and hypertrophy of preadipocytes were closely related to lipid deposition in animals. Butyric acid was reported to be involved in lipid metabolism. The aim of the current study was to investigate the effect of butyric acid on the proliferation and differentiation of the immortalized chicken preadipocyte 2 (ICP2). ICP2 were treated respectively with 12mM butyric acid for 48h in proliferation trial and 4mM butyric acid plus 200 μM oleic acid for 3 days in differentiation trial. For the proliferation trial, RNA-seq analysis revealed that 2039 genes were significantly up-regulated and 780 genes were significantly down-regulated with 12 mM butyric acid after 48 h treatment. Concurrently, Cell cycle, DNA replication and p53 signaling pathways were down-regulated in Butyric acid group. More importantly, 12 mM butyric acid restrained the expression of cell proliferation genes such as PCNA, CDK1 and CDK2 in Butyric acid group ( P <0.05), and the protein expression levels of PCNA and CDK1 were also significantly decreased ( P <0.05). The Oil red staining revealed a fewer presence of red fat droplets in ICP2 following treatment with 4 mM butyric acid, accompanied by decreased levels of total cholesterol (TC) and triglycerides (TG). RNA-seq analysis shown that the number of up and down-regulated genes were 2095 and 1042 respectively in OAB group (oleic acid+butyric acid) when compared with OA group (oleic acid). Meanwhile the AMPK signaling pathway, FOXO signaling pathway and focal adhesion were significantly enriched in OAB group. Additionally, 4 mM butyric acid inhibited the expression of lipid differentiation genes including FABP4, C/EBPα, PPARγ and LPL in OAB group ( P <0.05), as well as lipogenesis proteins such as FABP4, C/EBP-α and PPARγ ( P <0.05). In conclusion, 12 mM butyric acid effectively inhibited the proliferation of ICP2 by slowing down cell cycle progression, while 4 mM butyric acid alleviated lipid deposition by reducing the production of lipid droplets through inhibiting the expression of lipid differentiation marker genes and proteins.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yawen Zhang,Hongli Zhang,Jiangong Ma,Jing Zhao,Huajuan Yin,Xu Jing,Xiaowen Wang
With the advent of new-generation green extraction solvents, deep eutectic solvents (DESs) present significant prospects for the extraction of bioactive substances. In this study, saponins were extracted from oats using an ultrasonic-assisted method with green DESs, replacing conventional toxic organic solvents. Adding sodium chloride markedly reduced solubility, resulting in the separation of two phases from the extract: DES and water. The saponins were separated into the water phase, while the DES was reused in subsequent extractions, supporting extractant recycling and promoting sustainable development. Adding salt offers a more gentle, convenient, and cost-effective alternative to current phase separation methods, such as pH adjustment, temperature variation, and carbon dioxide or nitrogen gas application. Optimizing the extraction and recovery parameters yielded the highest oat saponin extraction efficiency at 18.73 mg g –1 , and the recovery was 86.4 %. When compared with ethanol extracts, DES extracts exhibited superior antioxidant activity. Lastly, the greenness of this extraction method was confirmed through three greenness evaluation programs of greenness. The extraction method based on emerging responsive solvents can be extended to other hydrophilic bioactive substances.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Huanhuan Jiang,Qibin Li,Yang Yang,Jingcong Dai,Li Wang,Richard D Cannon,Xiaojing Dong,Ting-li Han,Lina Hu
Short-chain fatty acids (SCFAs) are prominent regulators of host physiology and are closely related to the pathophysiology of many metabolic diseases. SCFAs are predominately generated by microbial fermentation of dietary fiber in the gut. Therefore, efficient, reproducible, and affordable analytical methods are needed to identify and quantify SCFAs in a complex fecal sample. In this study, five different SCFAs (acetic acid, butyric acid, propionic acid, valeric acid, and isobutyric acid) were compared using six different extraction methods (HCl-DE, NaOH-Hexane, H 3 PO 4 -Butanol, H 3 PO 4 -EA, NaHCO 3 -MTBE, and SPME) based on gas chromatography-mass spectrometry (GC-MS) analysis. The specificity, linearity, recovery, precision, and sensitivity of different analytical approaches were systematically assessed using SCFA chemical standards and further validated with human fecal samples, ensuring cross-species applicability. Our results revealed that no single method performed optimally for all SCFAs, but rather, distinct methods exhibited compound-specific advantages. Moreover, this study provides a practical guideline for balancing recovery, reproducibility, and extraction efficiency for method selection based on research purposes. The H 3 PO 4 -Butanol method displayed superior recovery accuracy for valeric acid (100.16 ± 0.81% and 101.82 ± 4.83% recovery rate for low and median spiked concentrations, respectively). It also exhibited minimum intra-day repeatability (0.92% to 5.67% RSD values) and good linearity (R 2 =0.94-0.99) across chemical mixtures. However, it showed the best extracted efficiency for butyric acid and best sensitivity for isobutyruc acid in fecal samples. Moreover, the H 3 PO 4 -EA method showed the highest recovery accuracy for isobutyric acid (103.21 ± 11.07% to 104.79 ± 3.59% recovery rate for all three different spiked concentrations) and the smallest inter-day repeatability (3.37% to 7.25% RSD values). The SPME method exhibited superior recovery accuracy for acetic acid (81.16 ± 21.17% to 94.08 ± 10.92% recovery rate for all three different spiked concentrations) and extracted the highest levels of SCFAs for acetic acid, propionic acid, and isobutyric acid. Finally, the application of these methods in human clinical samples revealed significant alterations of SCFA levels in patients with missed abortion, supporting their potential for translational studies. Overall, our results indicated that H 3 PO 4 -Butanol and SPME are the recommended methods for studying SCFAs in fecal samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2025
·
IF 5.4
Wenxiu Wang,Zhuo Liu,Ying He,Xue Wang,Jiabin Ma,Jingyi Ma,Pengfei Chang,Huidong Huang,Tian Yuan,Rui Guo,Yibing Ning,Zhigang Liu
Infant diarrhea remains a leading cause of morbidity and mortality worldwide. Although human milk oligosaccharides (HMOs) have been clinically linked to reduced pathogen colonization in mice, their preventive effects on infant diarrhea and the underlying mechanisms remain unclear. In this study, one-week-old diarrhea C57BL/6J mice were induced with enteropathogenic Escherichia coli O127 (EPEC O127) to establish an infant diarrhea model. Meanwhile, the mice received HMOs containing 2’-fucosyllactose (2’-FL, 1 g/kg) and/or lacto-N-neotetraose (LNnT, 0.3 g/kg) for three weeks. The results showed that HMOs significantly alleviated diarrhea symptoms, mitigated anxiety-like behaviors, reduced colon inflammation, and restored gut barrier integrity. Notably, HMOs reshaped the gut microbiota by increasing the relative abundance of short-chain fatty acids (SCFAs)-producing bacteria (e.g., Lacrimispora), thereby elevating fecal SCFAs levels while decreasing Escherichia and other harmful taxa. These findings suggest that HMOs can effectively relieve EPEC-induced infant diarrhea by modulating the composition and metabolic function of the gut microbiota. Overall, this study highlights the potential of HMOs as a dietary intervention for alleviating infant diarrhea and provides new insights into their application in infant health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2026
·
IF 5.8
Huan Tian,Feng-Lei Jiang
Perovskite nanocrystals (NCs) exhibit intrinsic chemical instability due to their ionic nature. This property renders them susceptible to structural degradation and optical performance deterioration upon exposure to various cations (both inorganic and organic ions). For instance, Cs+ can induce phase transformation and photoluminescence quenching in the CsPbBr3 NCs. In this work, we found that externally introduced metal cations exert diverse effects (classified as three types) on the structure and optical properties of CsPbBr3 NCs. Notably, certain cations, such as Zn2+ and Cd2+, can bind to the surface and shield nanocrystals against the detrimental effects of exogenous cations. The protection effects were observed by both optical spectroscopy and transmission electron microscopy. Pretreatment with both ZnBr2 and CdBr2 significantly enhances the resistance of CsPbBr3 NCs to structurally or optically detrimental exogenous cations (Cs+). Moreover, with didodecyldimethylammonium bromide (DDAB) simultaneously incorporated in this pretreatment, the pretreated CsPbBr3 NCs exhibit enhanced resistance to polar solvents (e.g., ethanol), manifesting as keeping stable and bright even when exposed to 20% (volume percentage) of ethanol. These three components (ZnBr2, CdBr2, and DDAB) exhibit complementary functions for chemical stability. This stability-preserving strategy is expected to enhance the robustness of perovskite NCs against complex chemical conditions encountered during the device fabrication process (solution processing and interlayer interface engineering) and operating environments.
✓ 已验证
产品关联
DOI
高影响因子
PHYTOTHERAPY RESEARCH
·
2026
·
IF 8.1
Long Cheng,Minghong Gan,Huiyang Wang,Yanru Song,Yang Bai,Dongfang Zhang
Type 2 diabetes (T2D) is a chronic disease that seriously threatens human health. Puerarin, the primary bioactive component of Pueraria lobata , alleviates T2D through multiple mechanisms, including protection of pancreatic β-cells, enhancement of insulin sensitivity, and antioxidant effects. However, its specific mechanisms of action against T2D remain incompletely understood. This study aimed to investigate the curative effects of puerarin in ameliorating glucose and lipid metabolism disorders in T2D mice by preserving gut microbiota homeostasis and promoting adipose tissue thermogenesis. To achieve this, 16S rRNA gene sequencing and metabolomics were employed to assess its effects on gut microbiota and fecal metabolites, while qRT-PCR and western blot were conducted to evaluate its impact on the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/peroxisome proliferator-activated receptor γ (PPARγ) signaling pathway. The results demonstrated that puerarin promoted gut microbiota homeostasis and elevated butyrate levels. Specifically, it increased the abundance of beneficial bacteria (Lactobacillaceae, Muribaculaceae, and Akkermansiaceae) and reduced the abundance of harmful bacteria (Desulfovibrionaceae, Marinifilaceae, and Helicobacteraceae). Moreover, puerarin enhanced brown adipose tissue (BAT) activity via the PI3K/AKT pathway and induced a browning phenotype in white adipose tissue (WAT) through the PPARγ pathway. These effects promoted mitochondrial biosynthesis, lipolysis, glucose utilization, and energy expenditure. Puerarin also increased glucagon-like peptide-1 (GLP-1) secretion, thereby further improving insulin sensitivity. In conclusion, this study confirms that puerarin improves glucose and lipid metabolism disorders in T2D mice by regulating gut microbiota homeostasis and promoting adipose tissue thermogenesis, which holds considerable potential for the treatment of T2D.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Liling Fu,Yanan Ren,Conghe Zhang,Wei Yang,Qisheng Tian,Guixiang Zhou,Tian Xu,Changhong Liu,Lei Zheng
In this study, n-butyric acid-modified high-amylose corn starch (NBA-HACS) with varying degrees of substitution (DS ≈ 0.1, 0.2, 0.3) was synthesized via carbodiimide-mediated esterification and employed to fabricate Pickering emulsions for resveratrol encapsulation and delivery. Structural characterization confirmed successful grafting of n-butyric acid onto the C-6 hydroxyl groups of native high-amylose corn starch (NA-HACS), enhancing particle hydrophobicity. The contact angle increased from 24.6° (NA-HACS) to 86.3° (NBA-HACS), indicating a pronounced enhancement in particle dual wettability. NBA-HACS3 formed stable Pickering emulsions at 10% (w/v) concentration and oil-to-water ratios of 4:6 and 5:5, maintaining structural integrity across pH 1–11 and remaining stable after 90 days of storage. NBA-HACS3 achieved high resveratrol encapsulation efficiency (90.3%) and exhibited excellent retention under thermal (37 °C, 35 days) and UV conditions (11 h ). In vitro digestion studies revealed that NBA-HACS3 emulsions enhanced resveratrol bioaccessibility to 38%, five times higher than the control. This resulted from efficient interfacial encapsulation enabled by increased hydrophobicity, enhanced acid resistance preserving droplet integrity under gastric conditions, and improved micellar solubilization during intestinal digestion. This establishes an effective modification strategy for enhancing starch-based particle emulsifying performance, offering a promising food-grade delivery system for hydrophobic bioactives in functional food applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2026
·
IF 5
Shuzhen Fang,Kangyi Zhang,Xiang Fang,Danqing Liu,Yulong Yang,Guijie Chen,Wenming Yang
Wilson's disease (WD) is a rare autosomal recessive disorder characterized by impaired copper metabolism and progressive multi‐organ damage. Emerging evidence suggests that gut microbiota dysbiosis and reduced microbial fermentation capacity may contribute to the progression of liver diseases. However, whether the gut microbiota can serve as a therapeutic target in WD remains unclear. This study aimed to investigate the modulatory effects of Lycium barbarum polysaccharides (LBPs), a bioactive component of the traditional Chinese medicine Gan‐Dou‐Fu‐Mu decoction, on gut microbiota composition and short‐chain fatty acids (SCFAs) production in WD, and to explore their potential as microbiota‐targeted prebiotic interventions. WD‐associated microbiota exhibited decreased microbial diversity and significantly lower SCFAs production, particularly acetic acid and total SCFAs, compared to healthy controls. LBPs supplementation significantly increased the relative abundances of beneficial genera, including Lactobacillus , Lacticaseibacillus , and Paraeggerthella , as well as SCFAs‐producing species such as Bacteroides fragilis and Lactobacillus delbrueckii . Notably, LBPs markedly enhanced acetic acid and total SCFA concentrations in WD microbiota, outperforming the standard treatment agent penicillamine. These findings highlight the promising prebiotic potential of LBPs and support their application as a novel microbiota‐targeted adjunctive therapy for WD. Schematic overview of the experimental workflow investigating the effects of LBPs on gut microbiota.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）