MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-12
·
IF 5.479
Tang Xiao,Zeng Xueyi,Liu Huimei,Yang Yili,Zhou Haibo,Cai Huaihong
A nanohybrid probe was fabricated from manganese dioxide nanosheets (MnO 2 NSs), molybdenum disulfide quantum dots (MoS 2 QDs) and o-phenylenediamine (OPD) for ratiometric detection of glutathione (GSH) in aqueous solutions and living cells. The MoS 2 QDs act as the fluorescent “turn off-on” units. The MnO 2 NSs have 3 functions, viz. (a) as fluorescence quencher, (b) as fluorescence initiator for oxidized OPD (ox OPD) and (c) as selective recognizer of GSH. The quenched blue fluorescence of the MoS 2 QDs can be restored by introducing GSH that reduces the MnO 2 NSs. However, the green fluorescence of ox OPD is decreased through the loss of peroxidase activity of MnO 2 NSs in the presence of GSH. Therefore, the ratio of the fluorescence intensities at 560 and 400 nm (from ox OPD and MoS 2 QDs, respectively) linearly decreases with increasing concentrations of GSH. Under the optimal conditions, the detection limit for GSH is as low as 90 nM. The method was successfully applied to the determination of GSH in human serum samples. This nanohybrid also is shown to be membrane-permeable and to have low cytotoxicity. This paved the way to intracellular sensing of GSH in living normal HFF and cancerous HeLa cells. Additionally, by combining with logic gate, this assay was successfully applied to visually discriminate changes in the intracellular GSH. The combination of ratiometric fluorometry and peroxidase mimicking can provide a wide range of application in bioanalysis and intracellular imaging.
Schematic representation of the ratiometric fluorometric detection and cellular imaging of glutathione using a nanohybrid composed of MoS 2 quantum dots and MnO 2 nanosheets with dual (blue and green emission and peroxidase mimicking properties.
研究领域:
生物分析
·
细胞生物学
·
医学诊断
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DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Xiaobin Zhang,Rongmei Kong,Qingqing Tan,Fei Qu,Fengli Qu
Glutathione (GSH) serves several vital biological functions and its abnormal levels are associated with many diseases. Simple and sensitive methods capable of detecting GSH are desired for better understanding the mechanism of diseases. In this work, a label-free MnO 2 nanosheets assisted aggregation-induced emission (AIE)-silica nanospheres (SiO 2 NPs) nanoprobe for fluorescent “turn-on” detection of GSH with high sensitivity was reported for the first time. The anionic tetraphenylethylene derivative 3 (TPE3) was synthesized and employed as the AIE-active probe, which can be aggregated on the uncoated amino-functionalized SiO 2 NPs to form AIE-SiO 2 NPs and emitted strong fluorescence. Negatively charged MnO 2 nanosheets were employed as both positive charge protectors for amino-functionalized SiO 2 NPs and recognition units for GSH. The presence of GSH could selectively reduce MnO 2 nanosheets to Mn 2+ , thus releasing the amino-functionalized SiO 2 NPs and exposure its positive charges. Accordingly, the TPE3 could be aggregated on the exposured amino-functionalized SiO 2 NPs to form AIE-SiO 2 NPs and emitted strong fluorescence. The proposed assay is simple, fast, low cost, and highly sensitive with a detection limit of 200 nM for GSH. The assay was further applied in human serum samples for the detection of GSH with satisfactory results, demonstrating its promising application in practical biological samples.
研究领域:
生物化学
·
分析化学
·
纳米医学
✓ 已验证
DOI
高影响因子
Energy Storage Materials
·
2018
·
IF 0
Jieqiong Qin,Sen Wang,Feng Zhou,Pratteek Das,Shuanghao Zheng,Chenglin Sun,Xinhe Bao,Zhong-Shuai Wu
Two-dimensional (2D) mesoporous nanosheets as electrodes present a critical materials platform for dramatically boosting the performance of planar micro-supercapacitors (MSCs) due to their unique features of interconnected porous network, enriched nanopore arrays, and high specific surface area. However, efficient strategies for constructing such complicated mesoporous architectures are very limited. Here, we developed a supramolecular bottom-up self-assembly strategy for direct synthesis of ultrathin mesoporous manganese dioxide (m-MnO 2 ) nanosheets for high-energy all-solid-state planar asymmetric MSCs (AMSCs). The m-MnO 2 nanosheets exhibited ultrathin thickness of 10 nm, uniformly interconnected network of mesopores with size of 5–15 nm, high surface area of 128 m 2 g −1 , and remarkably enhanced capacitance of 243 F g −1 at 1 mV s −1 in comparison with non-mesoporous MnO 2 nanosheets (123 F g −1 ). Further, all-solid-state AMSCs were assembled based on m-MnO 2 nanosheets as positive electrode , porous VN nanosheets as negative electrode , and electrochemically exfoliated graphene as conductive agent and metal-free current collector in “water-in-salt” gel electrolyte. Importantly, our all-solid-state AMSCs operated stably at 2.0 V and offered impressive energy density of 21.6 mWh cm −3 , outperforming most reported MnO 2 based MSCs, and two times higher than lithium thin-film batteries (≤10 mWh cm −3 ). Also, they presented long-term cycling stability with 90% capacity retention after 5000 cycles, outstanding flexibility without observable capacitance decay under different bending angles, and facile serial and parallel interconnection for creating high-voltage and high-capacitance integrated bipolar cells. Therefore, our proposed strategy will open many opportunites for patterning novel 2D mesoporous metal oxide nanosheets for high-performance microscale electrochemical energy storage devices.
研究领域:
材料科学
·
能源存储技术
✓ 已验证
DOI
MICROCHIMICA ACTA
·
2017
·Vol. 185
·期 1
·1-7
·
IF 4.58
Tan Qingqing,Zhang Ruirui,Kong Rongmei,Kong Weisu,Zhao Wenzhi,Qu Fengli
The authors describe a novel method for the determination of glutathione (GSH). Detection is based on target induced release of glucose from MnO 2 nanosheet-gated aminated mesoporous silica nanoparticles (MSNs). In detail, glucose is loaded into the pores of MSNs. Negatively charged MnO 2 nanosheets are assembled on the MSNs through electrostatic interactions. The nanosheets are reduced by GSH, and this results in the release of glucose which is quantified by using a commercial electrochemical glucose meter. GSH can be quantified by this method in the 100 nM to 10 μM concentration range, with a 34 nM limit of detection.
Glucose is loaded into the pores of mesoporous silica nanoparticles (MSNs). MnO 2 nanosheets are assembled on MSNs through electrostatic interactions. Glutathione (GSH) can reduce the nanosheets, and this results in the release of glucose which is quantified by using a commercial glucose meter.
研究领域:
分析化学
·
纳米医学
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Sustainable Food Systems
·
2025
·
IF 3.1
Chang Ma,Hooi Tin Ong,Hoon Koon Teoh,Yue Ling Wang,Zhong Han Zhu,Yan Liu,Juan Du
Background: T-2 toxin is a highly toxic mycotoxin that poses serious risks to food safety and human health. There is an urgent need for sensitive, selective, and reliable analytical methods for its detection in complex food matrices.Methods: We developed a novel ratiometric fluorescence immunoassay based on gold nanoparticles encapsulated in manganese dioxide nanosheets (AuNP@MnO₂), which exhibit intrinsic oxidase-like activity. The assay utilizes dual-emission fluorescence (at 430 nm and 570 nm) and quantifies T-2 toxin by measuring the fluorescence intensity ratio (F₄₃₀/F₅₇₀), thereby minimizing environmental and instrumental variability.Results: The method demonstrated excellent linearity over a wide concentration range (0.082–60.00 ng/mL) with a low detection limit of 0.068 ng/mL. High specificity was achieved, showing negligible cross-reactivity against common mycotoxins such as aflatoxin B₁, zearalenone, fumonisin B₁, ochratoxin A, and deoxynivalenol. In spiked corn samples, recoveries ranged from 76.94% to 99.48%, confirming good accuracy, stability, and reproducibility.Conclusion: This ratiometric immunoassay significantly enhances detection precision by self-calibrating through an internal fluorescence reference. Its simplicity, sensitivity, and robustness in real food samples make it a promising tool for rapid and reliable monitoring of T-2 toxin in food safety applications.
✓ 已验证
DOI
高影响因子
ACS Applied Energy Materials
·
2022
·
IF 6.959
Hui Peng,Faqiang Wang,Danyang Wang,Shuzhen Cui,Wenbo Hou,Guofu Ma
Aqueous Zn–MnO2 batteries have received much attention due to their low cost, safety, reliability, and large storage capacity. However, the sluggish ion/electron transport kinetics of bulk MnO2 inevitably decreases the rate capability and cycle stability of Zn–MnO2 batteries. Herein, hierarchical MnO2 nanosheet arrays self-anchored and grown in polyaniline-derived carbon nanotubes (PCNT@MnO2, denoted as PCMO) with enhanced stability are prepared by employing PCNT as both a substrate and a sacrificial reductant. The hollow PCNT conductive skeleton feature and robust integrated MnO2 nanosheet arrays in PCMO are not only favorable for enhancing electronic conductivity and accelerating electronic migration but also provide effective nucleation sites for the growth of MnO2 nanosheets and unimpeded ion channels, resulting in high specific capacity, rapid ion-diffusion kinetics, and long-term cycle stability. Specifically, the optimized PCMO material (PCMO-2) can deliver 264 mAh g–1 at 0.2 A g–1 after 120 cycles without obvious capacity decay, superior to the bare MnO2 material. Moreover, the as-fabricated PCMO//Zn battery can exhibit a high energy density of 320.2 Wh kg–1 at a power density of 120 W kg–1. The rational design of low-cost and integrated core–shell architecture with robust contact between MnO2 nanosheet arrays and a hollow conductive skeleton is expected to achieve cost-effective and high-performance Zn–MnO2 batteries.
研究领域:
材料科学
·
电化学
·
能源存储与转换
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 9
·1-9
·
IF 5.833
Ma Zerui,Li Pan,Jiao Meng,Shi Yu-e,Zhai Yongqing,Wang Zhenguang
Butyrylcholinesterase (BChE) can modulate the expression level of cholinesterase, which emerges as an important clinical diagnose index. However, the currently reported assays for BChE are suffering from the problem of interferences. A ratiometric fluorescence assay was developed based on the MnO 2 nanosheet (NS)–modulated fluorescence of sulfur quantum dots (S-dots) and o -phenylenediamine (OPD). MnO 2 NS can not only quench the fluorescence of blue emissive S-dots, but also enhance the yellow emissive OPD by catalyzing its oxidation reactions. Upon introducing BChE and substrate into the system, their hydrolysate can reduce MnO 2 into Mn 2+ , leading to the fluorescence recovery of S-dots and failure of OPD oxidation. BChE activity can be quantitatively detected by recording the change of fluorescence signals in the blue and yellow regions. A linear relationship is observed between the ratio of F 435 / F 560 and the concentration of BChE in the range 30 to 500 U/L, and a limit of detection of 17.8 U/L has been calculated. The ratiometric fluorescence assay shows an excellent selectivity to acetylcholinesterase and tolerance to various other species. The method developed provides good detection performances in human serum medium and for screening of inhibitors. Graphical abstract
研究领域:
分析化学
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生物化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jie Chen,Haishan Wu,Weiwei Zhang,Yun Huang,Jingcheng Zheng,Jiwei Shao,Yuwu Chi
The sensitive detection of glutathione (GSH) level is of great significance to disease diagnosis. However, commonly used fluorometry method usually suffers from limited sensitivity, potential interferences and inefficient detection. In this work, a smartphone-assisted and perovskite-based sensing platform for dual-readout detection of GSH was developed: the silica coated perovskite quantum dots (PQD@SiO 2 ) with a single particle level and high fluorescence efficiency were employed as fluorophore, which greatly avoids aggregation-caused quenching effect caused by multi-particles embedded in one SiO 2 shell, and the manganese dioxide (MnO 2 ) nanosheets were used as quencher. With the addition of GSH, the fluorescence intensity of PQD@SiO 2 -MnO 2 composites gradually enhances, while the color of solution turns from dark brown to light yellow under sunlight, which enables the dual-signal (fluorescence and colorimetric) and reliable detection of GSH level. In addition, the introduction of smartphones integrated image recording and data processing, further promotes the simple, visualized, cost-effective and real-time determination of GSH. The synthesis of PQD@SiO 2 at a single particle level, as well as the strategies of dual-mode and smartphone-assisted detection, holds the promising prospect in biosensing and chemical sensing.
研究领域:
生物传感
·
化学传感
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2023
·Vol. 13
·期 14
·2138
·
IF 5.3
Yanyun Cui,Jun Zhao,Huidan Li
Alkaline phosphatase (ALP) is among the most studied enzymes by far, playing an important role in the metabolism of organisms and the regulation of protein activity. Herein, a label-free composite nanoprobe is constructed by combining inorganic nanomaterials and aggregation-induced emission (AIE) molecule to achieve highly sensitive and selective detection of ALP. Negatively charged 9,10-bis [2-(6-sulfonatopropoxyl) naphthylethenyl] anthracene (BSNVA) molecule is synthesized, which has the AIE performance and can be assembled on the surface of amino–SiO2nanoparticles through electrostatic interaction for fluorescence enhancement. MnO2nanosheets are rich in negative charges, enabling them to be wrapped on the surface of the amino–SiO2nanosphere to shield the positive charge on its surface, making it impossible for BSNVA to accumulate on the surface and then weakening the bio-fluorescence of the system. Furthermore, with catalyzed substrates induced by ALP, generating ascorbic acid and the redox reaction between ascorbic acid and MnO2, the nanoprobe helps in realizing the high-sensitivity detection of ALP with a detection limit of 0.38 mU/mL. The proposed strategy requires no complex cleaning and modification processes and can overcome the quenching effect caused by the aggregation of traditional organic dyes, proving to be a simple, low-cost and “turn-on” fluorescent detection method for ALP.Keywords:aggregation-induced emission;fluorescence sensor;alkaline phosphatase;SiO2nanospheres;MnO2nanosheet
研究领域:
生物化学
·
医学检测
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW CARBON MATERIALS
·
2025
·
IF 6.8
Yan-bing YUAN,Zong-bin ZHAO,Hong-hui BI,Run-meng ZHANG,Xu-zhen WANG,Jie-shan QIU
For rechargeable aqueous zinc-ion batteries (ZIBs), the design of nanocomposites comprised of electrochemically active materials and carbon materials with novel structures has great promise in addressing the issue of electrical conductivity and structural stability in the electrode materials during electrochemical cycling. We report the production of a novel flexible electrode material, by anchoring MnO 2 nanosheets on a B,N co-doped carbon nanotube array (BNCNTs) grown on carbon cloth (BNCNTs@MnO 2 ), which was fabricated by in-situ pyrolysis and hydrothermal growth. The generated BNCNTs were strongly bonded to the surface of the carbon fibers in the carbon cloth which provides both excellent electron transport and ion diffusion, and improves the stability and durability of the cathode. Importantly, the BNCNTs offer more active sites for the hydrothermal growth of MnO 2 , ensuring a uniform distribution. Electrochemical tests show that BNCNTs@MnO 2 delivers a high specific capacity of 310.7 mAh g −1 at 0.1 A g −1 , along with excellent rate capability and outstanding cycling stability, with a 79.7% capacity retention after 8000 cycles at 3 A g −1 . Download: Download high-res image (181KB) Download: Download full-size image
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yang Zhou,Yuxin Wei,Runqiu Wang,Yanli Wei,Li Wang
A fluorescent sensing method is presented for the determination of L -Cysteine ( L -Cys) based on sulfur quantum dots (SQDs)/MnO 2 nanosheets (MnO 2 NS) nano system. SQDs was prepared from hydrothermal reaction from sublimed sulfur with β-Cyclodextrin as the surface ligand. SQDs have intense fluorescence which will be quenched in the presence of MnO 2 NS. After the addition of L -Cys, the redox reaction of MnO 2 NS and L -Cys results in the recovery of SQDs fluorescence. Under optimal conditions, the sensing system could achieve a sensitive detection of L -Cys with a wide linearity in the range of 8.0 μM-1.0 mM and a detection of limit as 2.44 μM (3σ/k). This sensing method also shows an excellent specificity and anti-inference, which has a great potential application for the real sample detection. The SQDs-MnO 2 NS served as a promising sensing system for the identification of L -Cys.
研究领域:
分析化学
·
纳米技术
·
生物传感
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Peng Miao,Yuguo Tang
Messenger RNA (mRNA) is a type of important gene regulation element and reliable biomarker for the early diagnosis of diseases. However, mRNA analysis in live cells cannot be easily realized since conventional techniques always require procedures like mRNA purification or cell fixation. Herein, we propose a powerful two-dimensional hybridization chain reaction (2D HCR) strategy for amplified sensing and imaging of intracellular mRNA. The basis is the design of ternary hairpin or dumbbell-structured DNA fuel strands. In the presence of mRNA, organized 2D DNA assembly reaction can be initiated, which leads to structural changes of a large number of fuel strands and produces an in situ 2D DNA network. An enhanced fluorescence signal is thus generated, allowing direct and quantitative analysis of mRNA with high signal-to-noise ratio. Moreover, MnO2 nanosheet/glutathione-aided transportation and release association are successfully applied to adapt the 2D HCR nanosystem in live cells. Therefore, this work provides a promising quantitative endogenous mRNA analysis tool for clinical applications.
研究领域:
分子生物学
·
纳米技术
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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