BIOSENSORS & BIOELECTRONICS
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2021
·
IF 10.618
He Cheng,Langlang Yi,Jianduo Wu,Guoqian Li,Gang Zhao,Zhixiang Xiao,Bo Hu,Lei Zhao,Jie Tian
Drug detection in biofluids has always been great importance for clinical diagnosis. Many detection technologies such as chromatography-mass spectrometry, have been applied to the detection of drugs. However, these technologies required multi-step operations, including complicated and time-consuming pretreatment processes and operations of bulky detection instruments, significantly limiting development of drug detection in clinical diagnosis. Herein, a portable 3D-printed paper cartridge was fabricated for fast sample preconcentration and direct drugs quantitative detection in biofluids by a portable Raman spectrometer. This cartridge contained both paper tip with silver nanowires to preconcentrate samples and achieve surface-enhanced Raman Scattering (SERS) measurement, and 3D-printed cartridge to build enclosed environment for the improvement of detection, which cost only one dollar. The preconcentration ability of the cartridge was up to 18.13-fold fluorescence enhancement and compared to the non-preconcentration method, it achieved 9.93-fold improvement of SERS performance. The anticancer drug of epirubicin hydrochloride, cyclophosphamide and their mixtures were quantitatively detected in the bovine serum or artificial urine. The integrated detection procedure required only 1 h, including sample pretreatment and preconcentration, drying, SERS measurements, and quantification analysis. This 3D-printed paper cartridge constituted a portable detection platform that would be potentially a practical and point-of-care detection tool for drug preconcentration and quantification on the clinical diagnosis.
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Fashion and Textiles
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2021
·Vol. 8
·期 1
·1-12
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IF 2.2
Chen Yuanli,Fan Hui,Zha Xinlin,Wang Wenwen,Wu Yi,Xiong Yi,Yan Kun,Wang Yuedan,Wang Dong
High efficiency and anti-pollution oil/water separation membrane has been widely explored and researched. There are a large number of hydroxyl groups on the surface of silica, which has good wettability and can be used for oil-water separation membranes. Hydrophilic silica nanostructures with different morphologies were synthesized by changing templates and contents of trimethylbenzene (TMB). Here, silica nanospheres with radical pores, hollow silica nanospheres and worm-like silica nanotubes were separately sprayed on the PVA -co- PE nanofiber membrane (PM). The abundance of hydroxyl groups and porous structures on PM surfaces enabled the absorption of silica nanospheres through hydrogen bonds. Compared with different silica nanostructures, it was found that the silica/PM exhibited excellent super-hydrophilicity in air and underwater “oil-hating” properties. The PM was mass-produced in our lab through melt-extrusion-phase-separation technique. Therefore, the obtained membranes not only have excellent underwater superoleophobicity but also have a low-cost production. The prepared silica/PM composites were used to separate n-hexane/water, silicone oil/water and peanut oil water mixtures via filtration. As a result, they all exhibited efficient separation of oil/water mixture through gravity-driven filtration.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2021
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IF 7.963
Qiang Chu,Shuang Zhang,Xin Yu,Yaxuan Wang,Mingkui Zhang,Xiaodong Zheng
Current studies simply focus on the toxicity of nano-plastics, while the correlation between their toxicity and bio-distribution, as well as intestinal microorganisms is still blank. Therefore, we systematically evaluated the toxicity based on the accumulation characteristics of nano-plastics in C. elegans . Meanwhile, for the first time, human fecal microbiota was transplanted into the gut of C. elegans and found that nano-plastics can through the intestinal barrier to the whole body after oral intake and can't be drastically excreted until die, thus causing toxic effects; while human fecal microbiota transplantation can significantly improve the living state via activating PMK-1/SKN-1 pathway to promote the production of intracellular glutathione, and exogenous glutathione addition can also markedly protect nematodes against nano-plastics induced toxicity. Our results not only provide a fully understand between the accumulation characteristic and health risk of nano-plastics, but also take C. elegans and intestinal flora into the field of toxicity evolution of nanomaterials.
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JOURNAL OF POLYMER SCIENCE
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2021
·Vol. 59
·期 11
·1065-1071
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IF 0
Yi-Ning Hu,Qiao-Mei Li,Yan-Feng Guo,Li-Jing Zhu,Zhi-Xiang Zeng,Zhu Xiong
Here, polyvinylidene fluoride (PVDF) membranes were fabricated via non-solvent induced phase separation (NIPS) using dopamine (DA) and polyethyleneimine (PEI) as the hydrophilic additives, which has a loose surface and somewhat improved hydrophilicity. Then nanofiltration (NF)-like thin-film composite forward osmosis (TFC FO) membrane with a loose polyamide (PA) active layer on the blend membrane was synthesized via the interfacial polymerization. The as-prepared NF-like TFC FO membrane exhibited a high water flux ( J w ) of 29.98 L m −2 h −1 and a much low specific salt flux ( J s /J w ) of 0.018 g/L, when 0.6 M NaCl was used as draw solution (DS). It had a superior rejection of malachite green (99.6% ± 0.1%) and a low rejection of NaCl (27.4% ± 4.2%), when filtrated malachite green/NaCl mixture solution in active layer-facing draw solution (AL-FS) mode. The results provide new insights on the design and preparation of FO membranes of selective separation for dyes from salty water. Graphical
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Insects
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2020
·Vol. 11
·期 11
·777
·
IF 2.22
Chengfeng Lei,Jian Yang,Jia Wang,Jia Hu,Xiulian Sun
Simple SummaryThe fall armyworm is a notorious lepidopteran pest that consumes many economically important crops. Its recent invasion into China threatens crops in over 19 provinces. This species is susceptible to its homologous nucleopolyhedrovirus (Spodoptera frugiperdamultiple nucleopolyhedrovirus, or SfMNPV). Selection of indigenous isolates that are adaptable in each geographical region is important for developing a virus-based pesticide. In this study, an SfMNPV field isolate was obtained from a natural population of the fall armyworm in Hubei, China. Two genotypes were cloned from the field isolate, and one genotype, SfHub-A, which had similar activity to the field isolate and produced significantly more progeny viruses, was considered to be a suitable strain for the commercial production of SfMNPV. This information will be valuable for developing a virus-based pesticide against fall armyworm in China.AbstractThe fall armyworm,Spodoptera frugiperda, is a new invading pest in China. The baculovirusSpodoptera frugiperdamultiple nucleopolyhedrovirus (SfMNPV) is a pathogenic agent of the fall armyworm and a potential agent for its control in integrated pest management strategies. In this work, we analyze the molecular and biological characteristics of an SfMNPV isolate collected from maize in China (SfMNPV-Hub). Two genotypes were further isolated from SfMNPV-Hub by an in vivo cloning method. The PstI profile of one genotype (SfHub-A) was similar to genotype A of the SfMNPV Colombian isolate, and the other (SfHub-E) was similar to genotype E of the Colombian isolate. The bioactivity of SfHub-A against second-instarS. frugiperdalarvae was not significantly different from that of SfMNPV-Hub, whereas SfHub-E was 2.7–5.5 fold less potent than SfMNPV-Hub. The speed of kill of SfHub-E was quicker than SfMNPV-Hub, while SfHub-A acted slower than SfMNPV-Hub. Occlusion body (OB) production of SfHub-A in anS. frugiperdacadaver was significantly higher than that of SfMNPV-Hub, while SfHub-E yielded far fewer occlusion bodies (OBs) in the host larvae. These results provide basic information for developing a virus-based pesticide against the invading pestS. frugiperda.Keywords:SfMNPV;field isolate;genotype;molecular characterization;biological activity;OB yield
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FOOD CHEMISTRY
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2021
·
IF 7.514
Qing Han,Ying Sun,Kangwei Shen,Yan Yan,Xuejun Kang
Based on packed-fiber solid-phase extraction and HPLC-DAD, a simple analytical method for the determination of seven synthetic dyes has been successfully developed. Polystyrene/polypyrrole (PS/PPy) fibers were obtained via electro-spinning of polystyrene skeletal nanofibers , followed by the oxidation with FeCl 3 to trigger the polymerization of pyrrole and the deposition of polypyrrole coatings on PS fibrous skeleton fibers. The relationship between the extraction performance of the fibers and the electrospinning process at different humidities was investigated based on morphologic study and BET surface area. In the extraction process, purification , concentration, and desorption could be accomplished in one step. The established method exhibited good sensitivity, selectivity, reproducibility, and good efficiency for synthetic dyes in casual snacks (preserved fruit, flavored yogurt, and fruity hard candy) samples. With optimal conditions, the LODs (S/N = 3) were 2.4 to 21.09 ng mL −1 , and linearities were acceptable in liquid matrix and solid matrices. The recoveries were 93.9–103.9%.
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CHEMICAL ENGINEERING SCIENCE
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2020
·
IF 3.871
Qi Wang,Xiaoli Wu,Jingjing Chen,Wenpeng Li,Haoqin Zhang,Jingtao Wang
Two-dimensional (2D) lamellar membranes hold a broad prospect for separation applications. However, their design and development need controllable fabrication of ultrathin and stable lamellar membranes. Here, an organic-inorganic lamellar composite membrane with layer-by-layer graphene oxide (GO) and polyethyleneimine (PEI) structure was fabricated through a dual-needle electrostatic atomization strategy. The GO nanosheets work as skeleton to ensure ultrathin and defect-free membrane structure, while PEI cross-links adjacent GO nanosheets for structure stability. Importantly, this membrane achieves fast polar solvent permeance: 46.2 and 146.8 L m −2 h −1 bar −1 for water and acetone, outperforming most reported GO-based membranes. Meanwhile, the ordered and cross-linked interlayer channels provide accurate size sieving for industrial dyes (larger than 1.6 nm) with the rejection of above 96%. Significantly, this lamellar composite membrane displays excellent structure, operation, pressure, and cycling stabilities, which are difficult for traditional lamellar membranes.
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MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Zeming Huang,Jiehao Lei,Hong Ruan,Yongyang Gong,Guan Wang,Li Zhou
Sulfur quantum dots (SQDs), the rising star in fluorescent nanomaterials, have captured increasing attention due to their unique attributes. However, the development of SQDs with targeted fluorescence response to analyte is still a challenge. Herein, we report a robust fluorescent SQDs material that possess both molecular recognition and fluorescence detection capability by employing hydroxypropyl-β-cyclodextrin (HP-β-CD) as ligand. The prepared SQDs simultaneously display fine aqueous dispersibility, stable and tunable fluorescence, and favorable biocompatibility, which enable them promising for applications in cellular imaging and sensing. Owing to the synergistic effect of inner filter effect (IFE) and host-guest interaction, the SQDs can be utilized as an effective fluorescent probe for tartrazine (TTZ) detection with excellent sensitivity and selectivity. The limit of detection can reach 82 nM with a wide linear range of 0–30 μM. Furthermore, the fluorescent SQDs were successfully utilized to detect TTZ in river water and food samples with satisfactory performance.
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Journal of Materials Chemistry A
·
2020
·Vol. 8
·期 18
·9160-9167
·
IF 11.301
Jingjing Chen,Jie Zhang,Xiaoli Wu,Xulin Cui,Wenpeng Li,Haoqin Zhang,Jingtao Wang,Xing-Zhong Cao,Peng Zhang
Interfacial polymerization is a classical method for the preparation of thin-film composite (TFC) membranes. However, facile and controllable fabrication remains a grand challenge. Here, a dual-needle electrostatic atomization strategy is presented to atomize aqueous and organic phases into droplets respectively, and then a polymerization reaction occurs at interfaces. This method shows excellent controllability over the membrane nanostructure: the monomer ratio determines compactness and the monomer amount governs thickness. Particularly, a hierarchically structured IP layer is developed by regulating the monomer ratio, where the loose layer, a low-resistance region, supports the formation of an ultrathin dense layer for rejection. And the hierarchical nanostructure is proved by positron annihilation spectroscopy, CO2 adsorption, etc. We demonstrate that an IP layer with a 13.8 nm-thick dense layer readily realizes ultrafast solvent permeance (56.9 and 23.7 L m−2 h−1 bar−1 for acetone and water, respectively) and complete rejection for acid yellow 14 (1.9 nm). Such a performance is superior to that of most reported TFC membranes and overcomes the general permeation–rejection trade-off effect. Moreover, this hierarchically structured membrane displays excellent operating stability, pressure stability and cycle stability, especially solvent resistance over 30 days. Briefly, such a strategy endows the membrane with accurate regulation from chemical components to the physical structure, paving the way for the design of next-generation TFC membranes.
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TALANTA
·
2020
·
IF 5.339
Biao Ma,Junjie Chi,Chengtao Xu,Ying Ni,Chao Zhao,Hong Liu
Perspiration contains valuable information indicating physiological health. For most wearable perspiration sensors, the sensing element contacts with skin directly. Yet lack of precise fluidic manipulation unit limits accurate and continuous analysis considering perspiration aggregation, evaporation loss and electrolyte reabsorption by sweat glands. The potential skin irritation caused by the chemicals in the sensor is also a safety concern. In this work, we report a wearable microfluidic device with fluidic manipulation unit based on capillary force to address these issues. Inspired by wicking materials for wiping perspiration in our daily life, herein, we use biocompatible threads to collect perspiration by capillary absorption. Then the collected perspiration was spontaneously delivered to a hydrophilic microfluidic channel, forming a continuous flow. Electrodes were embedded in the microfluidic channel for continuous electrochemical analysis and to avoid the direct skin contact. On-body tests demonstrated that continuous perspiration collection, transportation and analysis of Na + as a proof-of-concept analyte can be achieved using the pump-free epidermal microfluidic device.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Baoyi Wu,Yukun Jian,Xiaoxia Le,Han Lin,Shuxin Wei,Wei Lu,Jiawei Zhang,Afang Zhang,Chih-Feng Huang,Tao Chen
Inspired by the high importance of hollow structures in nature such as blood vessels and bamboos in matter transportation, properties enhancement, or even survival of living creatures, the creation of hollow materials remains of considerable interest. However, constructing hollow unique living-like soft and wet polymeric hydrogels with desirable structures and functionalities is still a big challenge. Here, we reported a robust and effective strategy to fabricate complex three-dimensional (3D) hollow polymeric hydrogel with designed shape and function diversity on the basis of supramolecular interactions. By placing a Ca2+ included gelatin core into the solution of alginate, hydrogel shell could be formed along with the shape of the gelatin core via coordination between alginate chains and Ca2+ diffused from gelatin. The hollow hydrogel could finally be obtained by dissolving the gelatin core. Various complex 3D hollow structures could be achieved by designing/constructing assembled gelatin core as a building block with adjustable supramolecular metal coordination position and strength. Moreover, hollow hydrogels with function diversity could be developed by introducing functional polymers or nanoparticles into the hydrogel wall. This work has made important progress in developing hollow polymeric hydrogel with desirable structures, shapes, and various functional applications including soft actuators and chemical reaction containers.
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PEST MANAGEMENT SCIENCE
·
2019
·Vol. 76
·期 4
·1363-1370
·
IF 3.255
Chengfeng Lei,Shili Yang,Wenxiao Lei,Innocent Nyamwasa,Jia Hu,Xiulian Sun
BACKGROUND Baculoviruses provide long-lasting control of crop pests and are harmless to humans and non-target animals, making them attractive bioinsecticides. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) has a wide-host range and is one such commercial bioinsecticide, but its low infectivity to older larvae and less-sensitive species precludes its large-scale application. We sought to improve the infectivity of AcMNPV. RESULTS Two enhancing factors, the truncated enhancin from Agrotis segetum granulovirus and GP37 from Cydia pomonella granulovirus, were expressed in fusion with the N-terminal and middle domain of the polyhedrin envelope protein of AcMNPV. Western blotting and immunoelectron microscopy analysis indicated that the enhancing factors were expressed on the occlusion bodies of the resulting AcMNPV variants. Bioassays showed that the median lethal doses of the recombinant viruses were 3.9-fold to 7.4-fold lower than those of the wild-type virus against the second and fourth instar of Spodoptera exigua larvae. The yields of occlusion bodies from the two recombinants in S. exigua larvae were comparable with those of the wild-type virus both in vitro and in vivo . Further bioassays showed that the AcMNPV variants fusing the enhancing factors were incapable of infecting the second instar larvae of S. litura , Helicoverpa armigera , and Pyrausta nubilalis , which were not sensitive to the wild-type AcMNPV. CONCLUSION These genetically modified AcMNPV variants exhibited an enhanced infectivity and may offer better baculovirus control of crop pests. © 2019 Society of Chemical Industry
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